Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0004162

Physical Location & Seq

Genomic Coordinates Forward (+)

56148251 .. 56152720

Gene Structure 4470 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG371900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2457 bp

ATGTTCGCAGCGTCCACCAGCATCTATACCCCCACCACCATCGCCCTCCTCCCTCCCTCCTCCGCTGATCGCAAATTAGCTTCTTTATGCACCGCCACCGCCACCGCCGCCACCACCACCACCACCACCAACAACAGGCGCACGCGCCGCCGTCCAATCACCAAATGCCGCGTGTCCACCTCCTCGCCCCTTTCTCTAAACCCTAAACCCTCCTCTTCCAATAACCACCTCCTCAAAAGCCTCGCCAAAACATTCGCCGGCGCCACCGCCGTCACCCTCATCCTAAACCTAACCAACTTCCCCGCCTTCATCGGCGGAGGCGGAGGAGGAGGTCATGGTTCCAATGGAGGCGGTGGAGGCGGCGGCGGAGAAGGAGGGTTTTGGAGGAGGCTGTTTCGCCTGCATGCGGCGAAAGCCGACGAGCCGAACGAGTCGTGGGACTCTCACGGGTTGCCGGGCGACATTGTGGTCCAGCTCAGCAGGCTCAGCGGCTTCAAGAAGTACAAGGTCTCGGAGGTCTTGTTCTTCGACAAGAAGCGGTGGAGCAGCGTCGGCGCCGACGACACCATCTCCGACATGGTGTCCCTCAGGCCCGGCGGCGTCTACACCAAGGCCGAGCTCCAGAACGAGCTCCAATCCCTGGCCAATTGCGGAATGTTCGAGAAGGTCGACTTCGAAGGCAAGACCAAGCCGGACGGCACCCTCGCCGTCACCGTCTCGTTCACGGAGAGCACGTGGCAGGCGGCGGAGAGGTTCCGGTGCATTAACGTGGGCCTGATGGCGCAGCCCAAGCCCATTGAGATGGACCCGGATATGACCGAGAGGGAGAAGCTCCAGTATTTCAGGAACCAGGAGAGGGATTACAAGAGGAGGATGGAGAGGGCCAGGCCGTGTTTGCTGCCGGCTCCGGTGCAGAGGGAGGTGTTGCAGATGGTGAGAGAGCAGGGGAAGGTGAGTGCTAGGGTGTTGCAGAAGATTCAGGCCCGGATTCTCAAGTGGTACCAAGATGAGGGGTATCTGTGTGCCAATGTTGTCAACTTTGGGAATTTGAATACAAAGGAGGTTGTGTGTGAGGTGGTGGAGGGCGATATTACTCGGCTGCTTATTCAGTTCCGGGATAAGCTTGGCAATGTTGTAGAGGGGAAGACTCAGAGTCTTGTGGTCAAGAGAGAATTGCCCAAACAGCTTCGACCAGGTAATGCGTTTAACATAGAAGCAGGGAAACAAGCTCTGAGGAACTTAACCTCCCTGGGATTGTTTTCAAACATTGAAGTGAATCCAGTACCAGATGAGAAGAATGAAGGAGGGATAGTTGTTGAAATAAATCTTAAAGAACAAGAGCACAAGACTGCTGAAGTAAGCACGGAATGGAACATTGTTCGTGGACATGGAGGCTATCCCACTTTGGCTTCACTGCTACCCGGTGGAACTGTTACATTTCAACATCGGAATATCAACGGATTGAATAGGTCTCTTATTGGTTCAGTAAGCAGTAGCAACCTCTACAACCTGCAGGTTCAGGATGATCTTGAGTTTAAGCTTGAGTACATGCATCCATATCTGGATGGTGTGGACAATCTCCGTGATCGCACTCTCCGGGTAAGCTGCTTTAACGGTCGAAAATTGAGTCCAGTCTTCACTGGTGGGCCAGGGGATGATGAAGTTCCCCCTGTATGGGTTGATCGCGCTGGCATGAAGGCTAATATAACAGAGAATTTGAGCCGTCAGAGCAAATTCACTTATGGTCTTGTGATGGAAGAGATAACCACACGTGATGAACAAAATCATATATGTTCAAATGGCCAAAGAGTGCTGCCCAGTGGAGGTGTCAGTGAAGATGGACCTCCAACAACTCTCAGCGGTACTGGCATTGACCATGTGGCATTTTTACAGTCAAATATCACACATGACAACACCAAGTTTGTGAATGGAACCTTAGTTGGTGAGAGGAATGTGTTTCAGGTTGACCAAGGCCTTGGCATTGGCAGCAAGTTTCCATTCTTCAACCGCCACCAGTTATCATTAACAAGATTTTTCCAGCTGAAGGAAGTGGAGGAAGGTGCTGGCAAACCACCACCTCCTGTTCTTGTACTTCATGGTCACTATGGTGGCTGTGTTGGAGACCTTCCAAGTTATGATGCATTTACCCTTGGGGGTCCATATTCTGTGAGAGGTTACAACATGGGTGAGATAGCCGCTGCTAGACAAATCCTCGAGCTTGCAGCTGAGCTACGGATACCGGTTAAAGGTAAAAATGTATATGCATTTGTAGAACATGGCAATGATCTCGGAAGTTCGAAGGATGTCAAGGGTAACCCAACAGAGGTATACAGGCGAGTGGGTCATGGGTCATCATATGGTGTTGGTGTCAAGTTAGGTCCAGTAAGAGTGGAATATGTTGTGGATCACAACTCCGGCACAGGTTCAACATTCTTCCGATTTGGTGACCGATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006605 GO:0006810 GO:0006886 GO:0006996 GO:0007275 GO:0008104 GO:0008150 GO:0009507 GO:0009526 GO:0009527 GO:0009536 GO:0009653 GO:0009657 GO:0009658 GO:0009662 GO:0009707 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009941 GO:0009987 GO:0010006 GO:0010033 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016043 GO:0017038 GO:0019867 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031350 GO:0031351 GO:0031354 GO:0031355 GO:0031358 GO:0031359 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042170 GO:0042221 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0048598 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0061927 GO:0065002 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072596 GO:0072598 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Bac_surface_Ag

Protein Analysis

818

Amino Acids

89.65

Weight (kDa)

8.9

Isoelectric Point (pI)

42.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
POTRA1_3_Toc75	PF25282	153 - 243	6.6e-30	Toc75-like, POTRA domain
POTRA2_Toc75	PF25280	252 - 363	1.2e-55	Toc75-like, second POTRA domain
POTRA1_3_Toc75	PF25282	364 - 445	1.9e-24	Toc75-like, POTRA domain
Omp85	PF01103	484 - 801	1.7e-17	Omp85 superfamily domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004162)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46740 AT4G09080 AT4G09080 AT4G09080
fragaria_vesca	FvH4_3g28760 FvH4_3g28760 FvH4_3g28760 FvH4_5g29410
malus_domestica	MD03G1086500.v1.1 MD11G1095600.v1.1
prunus_persica	Prupe.6G071100_v2.0.a1
pyrus_communis	pycom03g06860 pycom11g08100 pycom11g08110
rosa_chinensis	RchiOBHm_Chr5g0052291 RchiOBHm_Chr7g0222311
rosa_laevigata	RLG00000002071 RLG00000034828
rosa_multiflora	Rmu_sc0007069.1_g000009 Rmu_sc0028377.1_g000001 Rmu_sc0032875.1_g000001 Rmu_sc0032876.1_g000001
rosa_roxburghii	Rroxscaffold_1G00028120 Rroxscaffold_3G00237120
rosa_rugosa	Rorug05G0273100 Rorug07G0207600
rosa_samantha	Rh5CG382000 Rh5DG371900 Rh7AG349900 Rh7DG346100
rosa_wichuraiana	Rw5G032630 Rw5G032670 Rw7G029530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	467
Acc36I	ACCTGC	2 cut(s)	1504, 1518
Acc65I	GGTACC	1 cut(s)	999
AccB1I	GGYRCC	4 cut(s)	260, 554, 698, 999
AccB7I	CCANNNNNTGG	1 cut(s)	640
AccI	GTMKAC	3 cut(s)	603, 669, 2328
AccII	CGCG	3 cut(s)	145, 171, 1686
AclWI	GGATC	1 cut(s)	2412
AcoI	YGGCCR	2 cut(s)	642, 1801
AcsI	RAATTY	3 cut(s)	1045, 1714, 1733
AcuI	CTGAAG	2 cut(s)	1374, 2063
AcvI	CACGTG	2 cut(s)	735, 1772
AcyI	GRCGYC	3 cut(s)	261, 555, 600
AfaI	GTAC	6 cut(s)	503, 1001, 1284, 1547, 1864, 2091
AflIII	ACRYGT	1 cut(s)	1769
AgeI	ACCGGT	1 cut(s)	2239
AhdI	GACNNNNNGTC	2 cut(s)	580, 1152
AjnI	CCWGG	6 cut(s)	639, 849, 884, 1192, 1248, 1648
AleI	CACNNNNGTG	1 cut(s)	2106
AloI	GAACNNNNNNTCC	4 cut(s)	506, 538, 1229, 1261
Alw21I	GWGCWC	4 cut(s)	621, 633, 734, 1344
Alw26I	GTCTC	4 cut(s)	514, 721, 1476, 2115
AlwI	GGATC	1 cut(s)	2412
Ama87I	CYCGRG	1 cut(s)	2213
ApoI	RAATTY	3 cut(s)	1045, 1714, 1733
ArsI	GACNNNNNNTTYG	2 cut(s)	1612, 1644
AsiGI	ACCGGT	1 cut(s)	2239
Asp718I	GGTACC	1 cut(s)	999
AspLEI	GCGC	6 cut(s)	141, 147, 263, 557, 784, 1688
AsuC2I	CCSGG	7 cut(s)	456, 594, 809, 985, 1115, 1422, 1598
AsuHPI	GGTGA	8 cut(s)	151, 265, 703, 946, 964, 1955, 2198, 2456
AsuII	TTCGAA	2 cut(s)	675, 2297
AvaI	CYCGRG	1 cut(s)	2213
AvaII	GGWCC	5 cut(s)	469, 805, 1841, 2156, 2378
AxyI	CCTNAGG	1 cut(s)	587
BalI	TGGCCA	2 cut(s)	644, 1803
BanI	GGYRCC	4 cut(s)	260, 554, 698, 999
BanII	GRGCYC	2 cut(s)	621, 633
BbrPI	CACGTG	2 cut(s)	735, 1772
BbsI	GAAGAC	2 cut(s)	1151, 1627
Bbv12I	GWGCWC	4 cut(s)	621, 633, 734, 1344
BccI	CCATC	9 cut(s)	47, 575, 772, 796, 868, 925, 1559, 1747, 1832
BceAI	ACGGC	6 cut(s)	135, 254, 692, 712, 874, 1707
BcgI	CGANNNNNNTGC	2 cut(s)	2269, 2303
BciT130I	CCWGG	6 cut(s)	641, 851, 886, 1194, 1250, 1650
BciVI	GTATCC	1 cut(s)	2229
BcnI	CCSGG	7 cut(s)	456, 594, 809, 985, 1115, 1422, 1598
BcoDI	GTCTC	4 cut(s)	514, 721, 1476, 2115
BfaI	CTAG	2 cut(s)	960, 2202
BfmI	CTRYAG	1 cut(s)	1511
BfoI	RGCGCY	2 cut(s)	264, 558
BfuAI	ACCTGC	2 cut(s)	1504, 1518
BfuI	GTATCC	1 cut(s)	2229
BlpI	GCTNAGC	3 cut(s)	476, 485, 2226
Bme18I	GGWCC	5 cut(s)	469, 805, 1841, 2156, 2378
BmeRI	GACNNNNNGTC	2 cut(s)	580, 1152
BmeT110I	CYCGRG	1 cut(s)	2213
BmrI	ACTGGG	1 cut(s)	1812
BmsI	GCATC	3 cut(s)	30, 1561, 2128
BmuI	ACTGGG	1 cut(s)	1812
BpiI	GAAGAC	2 cut(s)	1151, 1627
BpmI	CTGGAG	2 cut(s)	605, 818
Bpu1102I	GCTNAGC	3 cut(s)	476, 485, 2226
Bpu14I	TTCGAA	2 cut(s)	675, 2297
BpuEI	CTTGAG	3 cut(s)	977, 1550, 1562
BpuMI	CCSGG	7 cut(s)	456, 594, 809, 985, 1115, 1422, 1598
BsaAI	YACGTR	2 cut(s)	735, 1772
BsaHI	GRCGYC	3 cut(s)	261, 555, 600
BsaI	GGTCTC	3 cut(s)	514, 1476, 2115
BsaJI	CCNNGG	8 cut(s)	609, 639, 1248, 1249, 1649, 1969, 1975, 2149
BsaWI	WCCGGW	3 cut(s)	756, 907, 2239
Bse118I	RCCGGY	3 cut(s)	257, 901, 2239
Bse1I	ACTGG	8 cut(s)	835, 1280, 1631, 1645, 1818, 1870, 2014, 2381
Bse21I	CCTNAGG	1 cut(s)	587
Bse3DI	GCAATG	2 cut(s)	1135, 2287
BseBI	CCWGG	6 cut(s)	641, 851, 886, 1194, 1250, 1650
BseDI	CCNNGG	8 cut(s)	609, 639, 1248, 1249, 1649, 1969, 1975, 2149
BseGI	GGATG	7 cut(s)	279, 879, 1528, 1552, 1570, 1660, 2308
BseMI	GCAATG	2 cut(s)	1135, 2287
BseMII	CTCAG	7 cut(s)	490, 499, 601, 1163, 1223, 1870, 2217
BseNI	ACTGG	8 cut(s)	835, 1280, 1631, 1645, 1818, 1870, 2014, 2381
BseRI	GAGGAG	9 cut(s)	38, 49, 172, 202, 221, 339, 342, 400, 883
BsgI	GTGCAG	1 cut(s)	932
Bsh1236I	CGCG	3 cut(s)	145, 171, 1686
Bsh1285I	CGRYCG	1 cut(s)	1618
BshNI	GGYRCC	4 cut(s)	260, 554, 698, 999
BshTI	ACCGGT	1 cut(s)	2239
BsiEI	CGRYCG	1 cut(s)	1618
BsiHKAI	GWGCWC	4 cut(s)	621, 633, 734, 1344
BsiHKCI	CYCGRG	1 cut(s)	2213
BslFI	GGGAC	2 cut(s)	452, 568
BsmAI	GTCTC	4 cut(s)	514, 721, 1476, 2115
BsmBI	CGTCTC	1 cut(s)	721
BsmFI	GGGAC	2 cut(s)	452, 568
Bso31I	GGTCTC	3 cut(s)	514, 1476, 2115
BsoBI	CYCGRG	1 cut(s)	2213
Bsp119I	TTCGAA	2 cut(s)	675, 2297
Bsp1286I	GDGCHC	4 cut(s)	621, 633, 734, 1344
Bsp143I	GATC	6 cut(s)	67, 1525, 1585, 1681, 2284, 2404
Bsp1720I	GCTNAGC	3 cut(s)	476, 485, 2226
BspCNI	CTCAG	7 cut(s)	489, 498, 600, 1162, 1224, 1869, 2218
BspFNI	CGCG	3 cut(s)	145, 171, 1686
BspMAI	CTGCAG	1 cut(s)	1515
BspMI	ACCTGC	2 cut(s)	1504, 1518
BspPI	GGATC	1 cut(s)	2412
BspT104I	TTCGAA	2 cut(s)	675, 2297
BspT107I	GGYRCC	4 cut(s)	260, 554, 698, 999
BspTNI	GGTCTC	3 cut(s)	514, 1476, 2115
BsrDI	GCAATG	2 cut(s)	1135, 2287
BsrFI	RCCGGY	3 cut(s)	257, 901, 2239
BsrI	ACTGG	8 cut(s)	835, 1280, 1631, 1645, 1818, 1870, 2014, 2381
BssAI	RCCGGY	3 cut(s)	257, 901, 2239
BssECI	CCNNGG	8 cut(s)	609, 639, 1248, 1249, 1649, 1969, 1975, 2149
BssMI	GATC	6 cut(s)	67, 1525, 1585, 1681, 2284, 2404
BssNAI	GTATAC	1 cut(s)	2329
BssNI	GRCGYC	3 cut(s)	261, 555, 600
BssT1I	CCWWGG	4 cut(s)	609, 1969, 1975, 2149
Bst1107I	GTATAC	1 cut(s)	2329
Bst2UI	CCWGG	6 cut(s)	641, 851, 886, 1194, 1250, 1650
Bst4CI	ACNGT	4 cut(s)	715, 1432, 1616, 1893
Bst6I	CTCTTC	2 cut(s)	220, 1752
BstACI	GRCGYC	3 cut(s)	261, 555, 600
BstBAI	YACGTR	2 cut(s)	735, 1772
BstBI	TTCGAA	2 cut(s)	675, 2297
BstDEI	CTNAG	8 cut(s)	476, 485, 587, 1149, 1232, 1856, 1936, 2226
BstEII	GGTNACC	2 cut(s)	2312, 2444
BstF5I	GGATG	7 cut(s)	279, 879, 1528, 1552, 1570, 1660, 2308
BstFNI	CGCG	3 cut(s)	145, 171, 1686
BstH2I	RGCGCY	2 cut(s)	264, 558
BstHHI	GCGC	6 cut(s)	141, 147, 263, 557, 784, 1688
BstKTI	GATC	6 cut(s)	70, 1528, 1588, 1684, 2287, 2407
BstMAI	GTCTC	4 cut(s)	514, 721, 1476, 2115
BstMBI	GATC	6 cut(s)	67, 1525, 1585, 1681, 2284, 2404
BstMCI	CGRYCG	1 cut(s)	1618
BstNI	CCWGG	6 cut(s)	641, 851, 886, 1194, 1250, 1650
BstNSI	RCATGY	2 cut(s)	407, 1552
BstPI	GGTNACC	2 cut(s)	2312, 2444
BstSFI	CTRYAG	1 cut(s)	1511
BstUI	CGCG	3 cut(s)	145, 171, 1686
BstV2I	GAAGAC	2 cut(s)	1151, 1627
BstXI	CCANNNNNNTGG	2 cut(s)	802, 1976
BstZ17I	GTATAC	1 cut(s)	2329
Bsu36I	CCTNAGG	1 cut(s)	587
BsuI	GTATCC	1 cut(s)	2229
BtgZI	GCGATG	1 cut(s)	25
BtsCI	GGATG	7 cut(s)	279, 879, 1528, 1552, 1570, 1660, 2308
BtsI	GCAGTG	1 cut(s)	1412
BtsIMutI	CAGTG	4 cut(s)	1412, 1638, 1825, 1837
BveI	ACCTGC	2 cut(s)	1504, 1518
CfoI	GCGC	6 cut(s)	141, 147, 263, 557, 784, 1688
Cfr10I	RCCGGY	3 cut(s)	257, 901, 2239
CseI	GACGC	2 cut(s)	538, 589
CsiI	ACCWGGT	1 cut(s)	1192
Csp6I	GTAC	6 cut(s)	502, 1000, 1283, 1546, 1863, 2090
CspAI	ACCGGT	1 cut(s)	2239
CviQI	GTAC	6 cut(s)	502, 1000, 1283, 1546, 1863, 2090
DdeI	CTNAG	8 cut(s)	476, 485, 587, 1149, 1232, 1856, 1936, 2226
DinI	GGCGCC	2 cut(s)	262, 556
DpnI	GATC	6 cut(s)	69, 1527, 1587, 1683, 2286, 2406
DpnII	GATC	6 cut(s)	67, 1525, 1585, 1681, 2284, 2404
DrdI	GACNNNNNNGTC	1 cut(s)	467
DriI	GACNNNNNGTC	2 cut(s)	580, 1152
DseDI	GACNNNNNNGTC	1 cut(s)	467
EaeI	YGGCCR	2 cut(s)	642, 1801
Eam1104I	CTCTTC	2 cut(s)	220, 1752
Eam1105I	GACNNNNNGTC	2 cut(s)	580, 1152
EarI	CTCTTC	2 cut(s)	220, 1752
EciI	GGCGGA	4 cut(s)	330, 336, 381, 761
Ecl136II	GAGCTC	2 cut(s)	619, 631
Eco130I	CCWWGG	4 cut(s)	609, 1969, 1975, 2149
Eco147I	AGGCCT	1 cut(s)	1974
Eco24I	GRGCYC	2 cut(s)	621, 633
Eco31I	GGTCTC	3 cut(s)	514, 1476, 2115
Eco47I	GGWCC	5 cut(s)	469, 805, 1841, 2156, 2378
Eco53kI	GAGCTC	2 cut(s)	619, 631
Eco57I	CTGAAG	2 cut(s)	1374, 2063
Eco72I	CACGTG	2 cut(s)	735, 1772
Eco81I	CCTNAGG	1 cut(s)	587
Eco88I	CYCGRG	1 cut(s)	2213
Eco91I	GGTNACC	2 cut(s)	2312, 2444
EcoICRI	GAGCTC	2 cut(s)	619, 631
EcoO65I	GGTNACC	2 cut(s)	2312, 2444
EcoRII	CCWGG	6 cut(s)	639, 849, 884, 1192, 1248, 1648
EcoT14I	CCWWGG	4 cut(s)	609, 1969, 1975, 2149
EcoT22I	ATGCAT	3 cut(s)	1554, 2143, 2266
EcoT38I	GRGCYC	2 cut(s)	621, 633
EgeI	GGCGCC	2 cut(s)	262, 556
EheI	GGCGCC	2 cut(s)	262, 556
ErhI	CCWWGG	4 cut(s)	609, 1969, 1975, 2149
Esp3I	CGTCTC	1 cut(s)	721
FalI	AAGNNNNNCTT	2 cut(s)	656, 688
FaqI	GGGAC	2 cut(s)	452, 568
FauI	CCCGC	1 cut(s)	310
FauNDI	CATATG	1 cut(s)	2356
FblI	GTMKAC	3 cut(s)	603, 669, 2328
FokI	GGATG	7 cut(s)	266, 886, 1535, 1539, 1577, 1667, 2315
FriOI	GRGCYC	2 cut(s)	621, 633
FspBI	CTAG	2 cut(s)	960, 2202
GlaI	GCGC	6 cut(s)	140, 146, 262, 556, 783, 1687
GsuI	CTGGAG	2 cut(s)	605, 818
HaeII	RGCGCY	2 cut(s)	264, 558
HgaI	GACGC	2 cut(s)	538, 589
HhaI	GCGC	6 cut(s)	141, 147, 263, 557, 784, 1688
Hin1I	GRCGYC	3 cut(s)	261, 555, 600
Hin6I	GCGC	6 cut(s)	139, 145, 261, 555, 782, 1686
HinP1I	GCGC	6 cut(s)	139, 145, 261, 555, 782, 1686
HincII	GTYRAC	3 cut(s)	670, 1036, 1966
HindII	GTYRAC	3 cut(s)	670, 1036, 1966
HindIII	AAGCTT	2 cut(s)	1121, 1538
HinfI	GANTC	8 cut(s)	431, 440, 976, 988, 1147, 1153, 1276, 1627
HphI	GGTGA	8 cut(s)	151, 265, 703, 946, 964, 1955, 2198, 2456
Hpy188I	TCNGA	8 cut(s)	514, 574, 1152, 1233, 1449, 1728, 2291, 2438
Hpy188III	TCNNGA	8 cut(s)	496, 622, 661, 844, 1165, 1520, 1529, 1562
Hpy99I	CGWCG	3 cut(s)	422, 554, 563
HpyCH4III	ACNGT	4 cut(s)	715, 1432, 1616, 1893
HpyCH4IV	ACGT	3 cut(s)	734, 768, 1771
HpyF3I	CTNAG	8 cut(s)	476, 485, 587, 1149, 1232, 1856, 1936, 2226
HpySE526I	ACGT	3 cut(s)	734, 768, 1771
Hsp92I	GRCGYC	3 cut(s)	261, 555, 600
HspAI	GCGC	6 cut(s)	139, 145, 261, 555, 782, 1686
KasI	GGCGCC	2 cut(s)	260, 554
KpnI	GGTACC	1 cut(s)	1003
KroI	GCCGGC	2 cut(s)	257, 901
KroNI	GCCGGC	2 cut(s)	259, 903
Kzo9I	GATC	6 cut(s)	67, 1525, 1585, 1681, 2284, 2404
LmnI	GCTCC	5 cut(s)	543, 624, 636, 837, 910
LweI	GCATC	3 cut(s)	30, 1561, 2128
MabI	ACCWGGT	1 cut(s)	1192
MaeI	CTAG	2 cut(s)	960, 2202
MaeII	ACGT	3 cut(s)	734, 768, 1771
MaeIII	GTNAC	7 cut(s)	271, 709, 1432, 2099, 2174, 2312, 2444
MalI	GATC	6 cut(s)	69, 1527, 1587, 1683, 2286, 2406
MboI	GATC	6 cut(s)	67, 1525, 1585, 1681, 2284, 2404
MfeI	CAATTG	1 cut(s)	646
MhlI	GDGCHC	4 cut(s)	621, 633, 734, 1344
MlsI	TGGCCA	2 cut(s)	644, 1803
MluCI	AATT	7 cut(s)	74, 646, 1045, 1172, 1622, 1714, 1733
MluNI	TGGCCA	2 cut(s)	644, 1803
Mly113I	GGCGCC	2 cut(s)	261, 555
MlyI	GAGTC	5 cut(s)	434, 440, 1141, 1162, 1636
MmeI	TCCRAC	3 cut(s)	597, 1871, 2098
Mox20I	TGGCCA	2 cut(s)	644, 1803
Mph1103I	ATGCAT	3 cut(s)	1554, 2143, 2266
MreI	CGCCGGCG	1 cut(s)	257
MroNI	GCCGGC	2 cut(s)	257, 901
MscI	TGGCCA	2 cut(s)	644, 1803
MseI	TTAA	8 cut(s)	765, 1206, 1241, 1329, 1536, 1611, 2024, 2244
MslI	CAYNNNNRTG	5 cut(s)	767, 800, 1271, 2106, 2280
Msp20I	TGGCCA	2 cut(s)	644, 1803
MspA1I	CMGCKG	6 cut(s)	65, 489, 1860, 2041, 2198, 2225
MunI	CAATTG	1 cut(s)	646
MvaI	CCWGG	6 cut(s)	641, 851, 886, 1194, 1250, 1650
MvnI	CGCG	3 cut(s)	145, 171, 1686
NaeI	GCCGGC	2 cut(s)	259, 903
NarI	GGCGCC	2 cut(s)	261, 555
NciI	CCSGG	7 cut(s)	456, 594, 809, 985, 1115, 1422, 1598
NdeI	CATATG	1 cut(s)	2356
NdeII	GATC	6 cut(s)	67, 1525, 1585, 1681, 2284, 2404
NgoMIV	GCCGGC	2 cut(s)	257, 901
NmeAIII	GCCGAG	2 cut(s)	640, 1075
NmuCI	GTSAC	4 cut(s)	271, 709, 2099, 2444
NsiI	ATGCAT	3 cut(s)	1554, 2143, 2266
NspI	RCATGY	2 cut(s)	407, 1552
NspV	TTCGAA	2 cut(s)	675, 2297
OliI	CACNNNNGTG	1 cut(s)	2106
PaeI	GCATGC	1 cut(s)	407
PaeR7I	CTCGAG	1 cut(s)	2213
PasI	CCCWGGG	1 cut(s)	1249
PceI	AGGCCT	1 cut(s)	1974
PcsI	WCGNNNNNNNCGW	5 cut(s)	426, 558, 666, 711, 731
PdiI	GCCGGC	2 cut(s)	259, 903
PfeI	GAWTC	3 cut(s)	976, 988, 1276
PflMI	CCANNNNNTGG	1 cut(s)	640
PfoI	TCCNGGA	1 cut(s)	1113
PinAI	ACCGGT	1 cut(s)	2239
PleI	GAGTC	5 cut(s)	434, 439, 1141, 1161, 1635
PluTI	GGCGCC	2 cut(s)	264, 558
PmaCI	CACGTG	2 cut(s)	735, 1772
PmlI	CACGTG	2 cut(s)	735, 1772
PpsI	GAGTC	5 cut(s)	434, 439, 1141, 1161, 1635
Ppu21I	YACGTR	2 cut(s)	735, 1772
Psp124BI	GAGCTC	2 cut(s)	621, 633
Psp6I	CCWGG	6 cut(s)	639, 849, 884, 1192, 1248, 1648
PspCI	CACGTG	2 cut(s)	735, 1772
PspEI	GGTNACC	2 cut(s)	2312, 2444
PspGI	CCWGG	6 cut(s)	639, 849, 884, 1192, 1248, 1648
PspXI	VCTCGAGB	1 cut(s)	2213
PstI	CTGCAG	1 cut(s)	1515
PvuII	CAGCTG	2 cut(s)	2041, 2225
RsaI	GTAC	6 cut(s)	503, 1001, 1284, 1547, 1864, 2091
RsaNI	GTAC	6 cut(s)	502, 1000, 1283, 1546, 1863, 2090
RseI	CAYNNNNRTG	5 cut(s)	767, 800, 1271, 2106, 2280
SacI	GAGCTC	2 cut(s)	621, 633
SalI	GTCGAC	1 cut(s)	668
SaqAI	TTAA	8 cut(s)	765, 1206, 1241, 1329, 1536, 1611, 2024, 2244
Sau3AI	GATC	6 cut(s)	67, 1525, 1585, 1681, 2284, 2404
SbfI	CCTGCAGG	1 cut(s)	1515
SchI	GAGTC	5 cut(s)	434, 440, 1141, 1162, 1636
SdaI	CCTGCAGG	1 cut(s)	1515
SduI	GDGCHC	4 cut(s)	621, 633, 734, 1344
SexAI	ACCWGGT	1 cut(s)	1192
SfaNI	GCATC	3 cut(s)	30, 1561, 2128
SfcI	CTRYAG	1 cut(s)	1511
SfoI	GGCGCC	2 cut(s)	262, 556
Sfr274I	CTCGAG	1 cut(s)	2213
SfuI	TTCGAA	2 cut(s)	675, 2297
SgrAI	CRCCGGYG	1 cut(s)	257
SinI	GGWCC	5 cut(s)	469, 805, 1841, 2156, 2378
SlaI	CTCGAG	1 cut(s)	2213
SmiMI	CAYNNNNRTG	5 cut(s)	767, 800, 1271, 2106, 2280
SmlI	CTYRAG	4 cut(s)	992, 1529, 1541, 2213
SmoI	CTYRAG	4 cut(s)	992, 1529, 1541, 2213
SphI	GCATGC	1 cut(s)	407
Sse8387I	CCTGCAGG	1 cut(s)	1515
Sse9I	AATT	7 cut(s)	74, 646, 1045, 1172, 1622, 1714, 1733
SseBI	AGGCCT	1 cut(s)	1974
SspDI	GGCGCC	2 cut(s)	260, 554
SspMI	CTAG	2 cut(s)	960, 2202
SstI	GAGCTC	2 cut(s)	621, 633
StuI	AGGCCT	1 cut(s)	1974
StyI	CCWWGG	4 cut(s)	609, 1969, 1975, 2149
TaaI	ACNGT	4 cut(s)	715, 1432, 1616, 1893
TaiI	ACGT	3 cut(s)	737, 771, 1774
TaqI	TCGA	8 cut(s)	528, 660, 669, 675, 1189, 1618, 2214, 2297
TaqII	GACCGA	1 cut(s)	833
TasI	AATT	7 cut(s)	74, 646, 1045, 1172, 1622, 1714, 1733
TatI	WGTACW	3 cut(s)	501, 1545, 2089
TfiI	GAWTC	3 cut(s)	976, 988, 1276
Tru1I	TTAA	8 cut(s)	765, 1206, 1241, 1329, 1536, 1611, 2024, 2244
Tru9I	TTAA	8 cut(s)	765, 1206, 1241, 1329, 1536, 1611, 2024, 2244
TscAI	CASTG	4 cut(s)	1419, 1645, 1825, 1837
TseFI	GTSAC	4 cut(s)	271, 709, 2099, 2444
Tsp45I	GTSAC	4 cut(s)	271, 709, 2099, 2444
TspDTI	ATGAA	6 cut(s)	298, 1314, 1674, 1709, 1791, 2084
TspGWI	ACGGA	5 cut(s)	740, 1379, 1473, 1571, 2248
TspRI	CASTG	4 cut(s)	1419, 1645, 1825, 1837
Van91I	CCANNNNNTGG	1 cut(s)	640
VpaK11BI	GGWCC	5 cut(s)	469, 805, 1841, 2156, 2378
XapI	RAATTY	3 cut(s)	1045, 1714, 1733
XceI	RCATGY	2 cut(s)	407, 1552
XcmI	CCANNNNNNNNNTGG	1 cut(s)	574
XhoI	CTCGAG	1 cut(s)	2213
XmiI	GTMKAC	3 cut(s)	603, 669, 2328
XspI	CTAG	2 cut(s)	960, 2202
Zsp2I	ATGCAT	3 cut(s)	1554, 2143, 2266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.