Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002318

Physical Location & Seq

Genomic Coordinates Forward (+)

8510018 .. 8513043

Gene Structure 3026 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1099500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 855 bp

ATGATGAAGGGGGAATCGTCACAGTCGGAGCCGATTCCGCTGCTAACAATAAGGCCAGGGGAAGAAGGAGAAGAACCCAGAAGCAGTGGCAGAAAAAGGCAACTGCAATTGCAATTGGTCACCCCCTCACGGTCATTCAAACTTCCCGAGGGTTGGCTGGTCGAGGAAAAGCCCCGTCCCCCTAGCTCTTCCCGCCCGGGCCATACCCATATCGACAAGTATTACTATGAGCCTGGCACGGGACTGAAGTTTCGTTCACTGGCAGCTGTTCAGAGATACCTAACAGAAGGGCAAATCGAACAACATACCAGAAGATCAAAACCAGGCAATGAGTGTACTGTGCTGATTACACCACGCACCAGGAGGACCAGTTCATCCTTTGTACTACCTGATGGTTGGGAAGTTGAGGAGAAGCCACGGAGCAATATTAATTATTCTGGTTATGTAGACAGGTATTATATTGAGCCAGGGACTAGAAAGCGGTTCCGTTCTTTATTATCTGTTGAGAGGTACCTAACAGAAGCAAATGAAGATGCTCCACTCAAGGCATTGATGCCTGCCAACAAGTCTAGTCTTTCGCCTGGCTCTGGCTTTCAAAAGATGAACAATTTGGGCGAGATCCTGTCCCAGAAAGTTATTTCTGGTTCTGTGAGGAGAAATCTTTCGGGCGATTATGATGCACCCTCCAATCCCAGCCCTCCAGCAAAAGTTAATTGGGTCCTCGGCGGTCCTGGCGGGAATATGTGGAACGCCTTCATGGACGACTCTAAGGTCCCGGATTCTGTAAAGCAAAAATGGTCCGAGACTTTTGTATCATCCGTCTCTGGTGAAAACGTGAGCGTGCCAAGGTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0008150 GO:0008327 GO:0009628 GO:0009893 GO:0010369 GO:0010604 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031325 GO:0036094 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043565 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0060255 GO:0065007 GO:0080090 GO:0080167 GO:0097159 GO:0098687 GO:1901265 GO:1901363 GO:1901535 GO:1901537

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MBD

Protein Analysis

285

Amino Acids

31.82

Weight (kDa)

9.47

Isoelectric Point (pI)

76.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MBD	PF01429	46 - 100	1.9e-06	Methyl-CpG binding domain
MBD	PF01429	122 - 177	1.4e-08	Methyl-CpG binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002318)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G59800
fragaria_vesca	FvH4_3g35002 FvH4_3g35002 FvH4_3g35010
malus_domestica	MD03G1099400.v1.1 MD03G1099500.v1.1 MD11G1113700.v1.1
prunus_persica	Prupe.6G084200_v2.0.a1 Prupe.6G084200_v2.0.a1 Prupe.6G084200_v2.0.a1 Prupe.6G084300_v2.0.a1 Prupe.6G084500_v2.0.a1
pyrus_communis	pycom03g08180 pycom03g08190 pycom11g09570
rosa_chinensis	RchiOBHm_Chr5g0062921 RchiOBHm_Chr5g0062931
rosa_laevigata	RLG00000009507 RLG00000035590 RLG00000035591
rosa_multiflora	Rmu_co7996734.1_g000001 Rmu_sc0000192.1_g000027 Rmu_sc0001738.1_g000035 Rmu_sc0003303.1_g000011 Rmu_sc0006171.1_g000008 Rmu_sc0010322.1_g000002 Rmu_sc0010322.1_g000003
rosa_roxburghii	Rroxscaffold_1G00017700 Rroxscaffold_1G00017710
rosa_rugosa	Rorug05G0352700 Rorug05G0352700 Rorug05G0352800 Rorug05G0352900
rosa_samantha	Rh5AG413800 Rh5AG413900 Rh5BG428000 Rh5BG428100 Rh5CG451600 Rh5CG451700 Rh5DG441700 Rh5DG441800
rosa_wichuraiana	Rw5G038880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	770
Acc65I	GGTACC	1 cut(s)	510
AccB1I	GGYRCC	1 cut(s)	510
AccI	GTMKAC	1 cut(s)	447
AciI	CCGC	5 cut(s)	38, 193, 481, 726, 735
AclWI	GGATC	1 cut(s)	613
AcuI	CTGAAG	1 cut(s)	266
AfaI	GTAC	3 cut(s)	337, 384, 512
AfiI	CCNNNNNNNGG	4 cut(s)	129, 153, 239, 587
AgsI	TTSAA	2 cut(s)	139, 596
AjnI	CCWGG	7 cut(s)	55, 232, 322, 359, 466, 580, 730
AloI	GAACNNNNNNTCC	2 cut(s)	355, 387
AluBI	AGCT	2 cut(s)	186, 266
AluI	AGCT	2 cut(s)	186, 266
Alw26I	GTCTC	2 cut(s)	797, 826
AlwI	GGATC	1 cut(s)	613
Ama87I	CYCGRG	2 cut(s)	146, 196
AoxI	GGCC	2 cut(s)	53, 199
ApeKI	GCWGC	2 cut(s)	40, 263
ArsI	GACNNNNNNTTYG	2 cut(s)	234, 266
AseI	ATTAAT	1 cut(s)	429
Asp700I	GAANNNNTTC	2 cut(s)	661, 752
Asp718I	GGTACC	1 cut(s)	510
AspS9I	GGNCC	6 cut(s)	199, 366, 718, 728, 772, 798
AsuC2I	CCSGG	3 cut(s)	197, 198, 776
AsuHPI	GGTGA	2 cut(s)	112, 839
AvaI	CYCGRG	2 cut(s)	146, 196
AvaII	GGWCC	5 cut(s)	366, 718, 728, 772, 798
BaeI	ACNNNNGTAYC	2 cut(s)	795, 828
BanI	GGYRCC	1 cut(s)	510
BbvI	GCAGC	2 cut(s)	27, 275
BccI	CCATC	1 cut(s)	386
BcgI	CGANNNNNNTGC	4 cut(s)	22, 56, 659, 693
BciT130I	CCWGG	7 cut(s)	57, 234, 324, 361, 468, 582, 732
BcnI	CCSGG	3 cut(s)	197, 198, 776
BcoDI	GTCTC	2 cut(s)	797, 826
BfaI	CTAG	3 cut(s)	183, 474, 570
BisI	GCNGC	2 cut(s)	41, 264
BlsI	GCNGC	2 cut(s)	42, 265
Bme18I	GGWCC	5 cut(s)	366, 718, 728, 772, 798
BmeT110I	CYCGRG	2 cut(s)	146, 196
BmgT120I	GGNCC	6 cut(s)	199, 366, 718, 728, 772, 798
BmiI	GGNNCC	5 cut(s)	30, 485, 512, 719, 774
BmsI	GCATC	3 cut(s)	523, 543, 667
BpmI	CTGGAG	1 cut(s)	684
BpuEI	CTTGAG	1 cut(s)	527
BpuMI	CCSGG	3 cut(s)	197, 198, 776
BsaJI	CCNNGG	7 cut(s)	56, 147, 196, 416, 467, 721, 845
BsaXI	ACNNNNNCTCC	2 cut(s)	355, 385
Bsc4I	CCNNNNNNNGG	4 cut(s)	129, 153, 239, 587
Bse1I	ACTGG	2 cut(s)	264, 369
Bse3DI	GCAATG	1 cut(s)	334
BseBI	CCWGG	7 cut(s)	57, 234, 324, 361, 468, 582, 732
BseDI	CCNNGG	7 cut(s)	56, 147, 196, 416, 467, 721, 845
BseGI	GGATG	2 cut(s)	374, 815
BseLI	CCNNNNNNNGG	4 cut(s)	129, 153, 239, 587
BseMI	GCAATG	1 cut(s)	334
BseNI	ACTGG	2 cut(s)	264, 369
BseRI	GAGGAG	2 cut(s)	422, 667
BseXI	GCAGC	2 cut(s)	27, 275
BseYI	CCCAGC	1 cut(s)	692
BshFI	GGCC	2 cut(s)	55, 201
BshNI	GGYRCC	1 cut(s)	510
BsiHKCI	CYCGRG	2 cut(s)	146, 196
BsiSI	CCGG	2 cut(s)	197, 776
BslFI	GGGAC	5 cut(s)	162, 255, 484, 610, 758
BslI	CCNNNNNNNGG	4 cut(s)	129, 153, 239, 587
BsmAI	GTCTC	2 cut(s)	797, 826
BsmBI	CGTCTC	1 cut(s)	826
BsmFI	GGGAC	5 cut(s)	162, 255, 484, 610, 758
BsnI	GGCC	2 cut(s)	55, 201
BsoBI	CYCGRG	2 cut(s)	146, 196
Bsp143I	GATC	2 cut(s)	314, 618
BspACI	CCGC	5 cut(s)	38, 193, 481, 726, 735
BspANI	GGCC	2 cut(s)	55, 201
BspLI	GGNNCC	5 cut(s)	30, 485, 512, 719, 774
BspPI	GGATC	1 cut(s)	613
BspQI	GCTCTTC	1 cut(s)	193
BspT107I	GGYRCC	1 cut(s)	510
BsrDI	GCAATG	1 cut(s)	334
BsrI	ACTGG	2 cut(s)	264, 369
BssECI	CCNNGG	7 cut(s)	56, 147, 196, 416, 467, 721, 845
BssMI	GATC	2 cut(s)	314, 618
BssT1I	CCWWGG	1 cut(s)	845
Bst2UI	CCWGG	7 cut(s)	57, 234, 324, 361, 468, 582, 732
Bst4CI	ACNGT	3 cut(s)	24, 132, 340
Bst6I	CTCTTC	1 cut(s)	193
BstC8I	GCNNGC	2 cut(s)	558, 842
BstDEI	CTNAG	1 cut(s)	768
BstDSI	CCRYGG	1 cut(s)	416
BstEII	GGTNACC	1 cut(s)	118
BstF5I	GGATG	2 cut(s)	374, 815
BstKTI	GATC	2 cut(s)	317, 621
BstMAI	GTCTC	2 cut(s)	797, 826
BstMBI	GATC	2 cut(s)	314, 618
BstMWI	GCNNNNNNNGC	3 cut(s)	37, 192, 732
BstNI	CCWGG	7 cut(s)	57, 234, 324, 361, 468, 582, 732
BstPI	GGTNACC	1 cut(s)	118
BstV1I	GCAGC	2 cut(s)	27, 275
BstX2I	RGATCY	1 cut(s)	618
BstYI	RGATCY	1 cut(s)	618
BsuRI	GGCC	2 cut(s)	55, 201
BtgI	CCRYGG	1 cut(s)	416
BtsCI	GGATG	2 cut(s)	374, 815
BtsI	GCAGTG	1 cut(s)	91
BtsIMutI	CAGTG	2 cut(s)	91, 257
Cac8I	GCNNGC	2 cut(s)	558, 842
Cfr13I	GGNCC	6 cut(s)	199, 366, 718, 728, 772, 798
Cfr9I	CCCGGG	1 cut(s)	196
Csp6I	GTAC	3 cut(s)	336, 383, 511
CviAII	CATG	1 cut(s)	757
CviQI	GTAC	3 cut(s)	336, 383, 511
DdeI	CTNAG	1 cut(s)	768
DpnI	GATC	2 cut(s)	316, 620
DpnII	GATC	2 cut(s)	314, 618
DrdI	GACNNNNNNGTC	1 cut(s)	770
DseDI	GACNNNNNNGTC	1 cut(s)	770
Eam1104I	CTCTTC	1 cut(s)	193
EarI	CTCTTC	1 cut(s)	193
Eco130I	CCWWGG	1 cut(s)	845
Eco47I	GGWCC	5 cut(s)	366, 718, 728, 772, 798
Eco57I	CTGAAG	1 cut(s)	266
Eco88I	CYCGRG	2 cut(s)	146, 196
Eco91I	GGTNACC	1 cut(s)	118
EcoO109I	RGGNCCY	2 cut(s)	718, 772
EcoO65I	GGTNACC	1 cut(s)	118
EcoRII	CCWGG	7 cut(s)	55, 232, 322, 359, 466, 580, 730
EcoT14I	CCWWGG	1 cut(s)	845
ErhI	CCWWGG	1 cut(s)	845
Esp3I	CGTCTC	1 cut(s)	826
FaeI	CATG	1 cut(s)	760
FaiI	YATR	9 cut(s)	204, 210, 228, 306, 444, 459, 675, 743, 758
FaqI	GGGAC	5 cut(s)	162, 255, 484, 610, 758
FatI	CATG	1 cut(s)	756
FauI	CCCGC	2 cut(s)	200, 728
FblI	GTMKAC	1 cut(s)	447
Fnu4HI	GCNGC	2 cut(s)	41, 264
FokI	GGATG	2 cut(s)	361, 802
Fsp4HI	GCNGC	2 cut(s)	41, 264
FspBI	CTAG	3 cut(s)	183, 474, 570
GluI	GCNGC	2 cut(s)	41, 264
GsaI	CCCAGC	1 cut(s)	696
GsuI	CTGGAG	1 cut(s)	684
HaeIII	GGCC	2 cut(s)	55, 201
HapII	CCGG	2 cut(s)	197, 776
Hin1II	CATG	1 cut(s)	760
HinfI	GANTC	4 cut(s)	14, 34, 764, 779
HpaII	CCGG	2 cut(s)	197, 776
HphI	GGTGA	2 cut(s)	112, 839
Hpy166II	GTNNAC	3 cut(s)	257, 336, 448
Hpy188I	TCNGA	3 cut(s)	28, 273, 802
Hpy188III	TCNNGA	1 cut(s)	146
Hpy8I	GTNNAC	3 cut(s)	257, 336, 448
HpyAV	CCTTC	3 cut(s)	59, 281, 763
HpyCH4III	ACNGT	3 cut(s)	24, 132, 340
HpyCH4IV	ACGT	1 cut(s)	834
HpyCH4V	TGCA	3 cut(s)	106, 112, 680
HpyF10VI	GCNNNNNNNGC	3 cut(s)	37, 192, 732
HpyF3I	CTNAG	1 cut(s)	768
HpySE526I	ACGT	1 cut(s)	834
Hsp92II	CATG	1 cut(s)	760
KpnI	GGTACC	1 cut(s)	514
Kzo9I	GATC	2 cut(s)	314, 618
LguI	GCTCTTC	1 cut(s)	193
LmnI	GCTCC	3 cut(s)	28, 420, 541
Lsp1109I	GCAGC	2 cut(s)	27, 275
LweI	GCATC	3 cut(s)	523, 543, 667
MaeI	CTAG	3 cut(s)	183, 474, 570
MaeII	ACGT	1 cut(s)	834
MaeIII	GTNAC	2 cut(s)	18, 118
MalI	GATC	2 cut(s)	316, 620
MboI	GATC	2 cut(s)	314, 618
MboII	GAAGA	5 cut(s)	74, 83, 180, 324, 542
MfeI	CAATTG	2 cut(s)	107, 113
MflI	RGATCY	1 cut(s)	618
MluCI	AATT	5 cut(s)	107, 113, 430, 607, 712
MlyI	GAGTC	1 cut(s)	758
MmeI	TCCRAC	1 cut(s)	6
MroXI	GAANNNNTTC	2 cut(s)	661, 752
MseI	TTAA	3 cut(s)	429, 711, 853
MspA1I	CMGCKG	2 cut(s)	40, 266
MspI	CCGG	2 cut(s)	197, 776
MunI	CAATTG	2 cut(s)	107, 113
MvaI	CCWGG	7 cut(s)	57, 234, 324, 361, 468, 582, 732
MwoI	GCNNNNNNNGC	3 cut(s)	37, 192, 732
NciI	CCSGG	3 cut(s)	197, 198, 776
NdeII	GATC	2 cut(s)	314, 618
NlaIII	CATG	1 cut(s)	760
NlaIV	GGNNCC	5 cut(s)	30, 485, 512, 719, 774
NmeAIII	GCCGAG	1 cut(s)	702
NmuCI	GTSAC	2 cut(s)	18, 118
PciSI	GCTCTTC	1 cut(s)	193
PdmI	GAANNNNTTC	2 cut(s)	661, 752
PfeI	GAWTC	3 cut(s)	14, 34, 779
PfoI	TCCNGGA	1 cut(s)	774
PkrI	GCNGC	2 cut(s)	42, 265
PleI	GAGTC	1 cut(s)	758
PpsI	GAGTC	1 cut(s)	758
PpuMI	RGGWCCY	2 cut(s)	718, 772
PshBI	ATTAAT	1 cut(s)	429
Psp5II	RGGWCCY	2 cut(s)	718, 772
Psp6I	CCWGG	7 cut(s)	55, 232, 322, 359, 466, 580, 730
PspEI	GGTNACC	1 cut(s)	118
PspFI	CCCAGC	1 cut(s)	692
PspGI	CCWGG	7 cut(s)	55, 232, 322, 359, 466, 580, 730
PspN4I	GGNNCC	5 cut(s)	30, 485, 512, 719, 774
PspPI	GGNCC	6 cut(s)	199, 366, 718, 728, 772, 798
PspPPI	RGGWCCY	2 cut(s)	718, 772
PsuI	RGATCY	1 cut(s)	618
PvuII	CAGCTG	1 cut(s)	266
RsaI	GTAC	3 cut(s)	337, 384, 512
RsaNI	GTAC	3 cut(s)	336, 383, 511
SapI	GCTCTTC	1 cut(s)	193
SaqAI	TTAA	3 cut(s)	429, 711, 853
SatI	GCNGC	2 cut(s)	41, 264
Sau3AI	GATC	2 cut(s)	314, 618
Sau96I	GGNCC	6 cut(s)	199, 366, 718, 728, 772, 798
SchI	GAGTC	1 cut(s)	758
SfaNI	GCATC	3 cut(s)	523, 543, 667
SinI	GGWCC	5 cut(s)	366, 718, 728, 772, 798
SmaI	CCCGGG	1 cut(s)	198
SmlI	CTYRAG	1 cut(s)	542
SmoI	CTYRAG	1 cut(s)	542
SrfI	GCCCGGGC	1 cut(s)	198
Sse9I	AATT	5 cut(s)	107, 113, 430, 607, 712
SsiI	CCGC	5 cut(s)	38, 193, 481, 726, 735
SspI	AATATT	1 cut(s)	427
SspMI	CTAG	3 cut(s)	183, 474, 570
StyI	CCWWGG	1 cut(s)	845
TaaI	ACNGT	3 cut(s)	24, 132, 340
TaiI	ACGT	1 cut(s)	837
TaqI	TCGA	3 cut(s)	162, 213, 297
TasI	AATT	5 cut(s)	107, 113, 430, 607, 712
TatI	WGTACW	2 cut(s)	335, 382
TfiI	GAWTC	3 cut(s)	14, 34, 779
Tru1I	TTAA	3 cut(s)	429, 711, 853
Tru9I	TTAA	3 cut(s)	429, 711, 853
TscAI	CASTG	2 cut(s)	91, 264
TseFI	GTSAC	2 cut(s)	18, 118
TseI	GCWGC	2 cut(s)	40, 263
Tsp45I	GTSAC	2 cut(s)	18, 118
TspDTI	ATGAA	5 cut(s)	20, 363, 543, 617, 745
TspGWI	ACGGA	3 cut(s)	433, 476, 808
TspMI	CCCGGG	1 cut(s)	196
TspRI	CASTG	2 cut(s)	91, 264
VpaK11BI	GGWCC	5 cut(s)	366, 718, 728, 772, 798
VspI	ATTAAT	1 cut(s)	429
XmaI	CCCGGG	1 cut(s)	196
XmiI	GTMKAC	1 cut(s)	447
XmnI	GAANNNNTTC	2 cut(s)	661, 752
XspI	CTAG	3 cut(s)	183, 474, 570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.