Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001925

Physical Location & Seq

Genomic Coordinates Forward (+)

19544710 .. 19562706

Gene Structure 17997 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1161700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3816 bp

ATGTACATAAAGCAGGTTATTATTGAAGGATTTAAGAGCTACAGGGAACAAGTTGCTACTGAGGCCTTCAGCCCAAAAGTTAATTGTGTTGTTGGTGCCAATGGATCTGGAAAGACGAACTTCTTCCATGCGATCCGGTTTGTACTAAGTGACCTTTTCCAAAACCTTCGAAGCGAGGATAGGCATGCTCTACTCCATGAAGGTGCAGGGCACCAAGTAATATCTGCGTTTGTGGAGATTGTCTTTGATAATGCTGACAATCGAATCCCGGTTGATAAGGAGGAAGTGCACTTGCGAAGAACCATTGGTTTGAAGAAGGATGAGTATTTCTTGGATGGAAAGCATATCACGAAAACGGAAGTTATGAATTTACTAGAAAGTGCTGGGTTCTCTCGCTCCAATCCTTATTATGTTGTGCAGCAAGGAAAGATAGCATCCTTGACACTGATGAAAGACTCTGAACGTCTGGATTTACTGAAAGAAATTGGTGGTACTCGAGTGTATGAGGAGAGGCGTCGAGAAAGTTTGAAAATTATGCAGGAAACTGGCAAGTCATTATTGAATTATTTGTTTATGATAGTTCAAGTTGTTCAGTACTTGGATGAGAGATTGAAGGAGTTGGATGAAGAGAAAGAGGAACTAAGACAGTATCAGCAATTTGACAAGCAGCGGAAATCTTTGGAATACACTATTTATGACAAGGAACTTCAGGATGCTCGCCAAAAGTTGGCAGAGGTAGAAGATGCTAGAAATAAGGTTTCTGAAACTTCAACAAAGATGTATAATACAGTGCTGGATGCCCATGAAAAGTCTAAGGACCTGGACAAGATTTTGAAGGATCTGACGAAAGAGCTTCAAGCTTTAAATAAGGATAAAGAAGCAATAGAAATGCGAAGAACAGAAGCAATAAAGAAGCGCACAGAGCTTCAGCTTGATGTCAACGATTTGGAGGAGAAGAAAACTGGAAACACCCGGGTCAAAGAAGATGCTGTGAAACAGCTGCAGAGTCTGCAGAAAGAAATTAAGGATTCAGAGGAAGAACTTAAGAAAATAAATCCTCTGTATGATAATCAAGTCAAGATGGAAATGGAGATAACAAAAGGAATAATGGAACGCGAGAAGCAGCTTAGCATACTGTACCAAAAACAAGGTCGTGCCACCCAGTTTTCAAGTAAAGCTGCTCGTGACAAATGGCTCCAAAAGGAAATTGATGACCTTGAACGAGTTCTTTCTTCAAATTTGGCACAGCTCTGGAGTTATGTTGAGCAAAAACTTAAAGATGAAATTGCACGGCTTGATGCTGAGTTGAGTGACAGGGATGTCTACGTTGAGTCTCGAAGAACTGAGATTGCCACTATTGAATCTCTCATCTCTCAGTCACATGATGGGTTTGGTCATCACAAGACACAGAGGGACAAGATGCAGGATGAGCGCAAGTCCCTGTGGAGGAAAGAAACTGAACTTTCTACTGAAATTGAGAAGCTGAGAACAGAAGTTGAAAAGGCTGAAAAGAGCCTTGATCATGCAACTCCGGGTGATGTGAGGAGAGGGCTAAATTCTGTTCGTAAGATCTGCAGAGAGTATAATATACGTGGGGTATTTGGTCCAATCATTGAGTTGCTTGATTGTGACGAAAAGTTTTTTACAGCCGTTGAAGTAACTGCTGGGAACAGCTTGTTTCACGTTGTGGTTGAAAATGACGAAATATCAACCCAGATAATTAGGCACCTTAATTCATTGAAAGGTGGACGAGTTACATTTATTCCCCTCAATAGGGTGAAGGCTCCACGGGTAACTTATCCACAGAATTCAGATGTGGTTCCTCTGTTGAAGAAATTAAAATTTGCTCCGAACTATACCCCGGCATTTTCCCAGATTCATTATATCATTTTGGTAGTTGAGCTATTGTGGTCAAACCTTGGTTGTGATGGATCTGGCAATTATTACATCAAAAGTGGACCTTTTTTTGTATTTGCAAGAACGGTTGTTTGTCGAGATATGGATGTGGCTACAAGGGTTGCTCGTAATGATGGTCTAGACTGCATTACTTTGGAAGGTGACCAAGTCAGCAAGAAGGGTGGCATGACGGGAGGATTTTATGACCATAGACGGTCAAAATTGAAGTTTATGCATATAATCATGCAGAATACAAAATCTATCAACATGAAGGAAGAAGAGCTGGAGAAAGTAAAGAAAATGCTTGAAGATATCCTTTTGGAAAATGATTTCATATACACGCAGAGCAATTGGGGTGTTGTTTCTTTCTCTTCCTTCTTTATAGACAAAAAAATTACAGAACTCGTAACTGAACAGCAGAAAATTGATGCCAAGCGTGCCCATGACAAATCAGAAATGGAACAACTTAAGCAGGACATTGCTAATGCTAATAAGCAGAAGAGTCTGATTTCCAAAGCTTGTGGGAATAAGAAAAAATCACTGGCGGATGTGGAGACTCAAATTACTCAGCTTAGAGCCAGTGTGGCCATGAAGAGAGCTGAAATGGGCACAGATCTCCTTGATCATTTATCTCCAGAGGAGAAGGATCTTCTCTCAAGATTAAACCCTGAAATAGCGGATCTTAAAGAGAAGCTTGTTTCATGCAAGACGGATCGTTTTGAGACTGAAACAATAAAGGCAGAGCTGGAGACTAATTTAACCACAAACCTTGAGAGGCGGAGGCAAGAACTAGAGGCCATAATATCTTCTGTGGAGACTGACAATTATAATGGTGAAGCTGAAATTAAGACTCAGGAACTTAATGATGCTGAATTATTGGTTAAAGATGCAACAGAGCAGCTCAAAAGAGTTTCTGAAACTATAGATAGCAAGTCAAAGCATCTCAAGAGAATTAAAGATGAAAAGACTGCGTTAAAGACTTTGGAAGACAAGTATGAGAGGACACTTCAGGATGAAGCCAAAGAGCTAGAGCAACTGTTGAGTAAAAGAAATATGTTTCTTGCAAAACAAGAAGAATACTCAAAGAAAATCAGGGAATTAGGTCCATTGTCATCGGATGCTTTTGAAGCGTATAAGCGAAGGGGCATCAAAGAGTTGCATAAAGCGCTGCATAGATGCAATGAACAACTGCAACAGTTTAGTCATGTCAACAAGAAAGCACTTGATCAATATGTAAATTTTACAGAGCAACGAGAAGAGCTCCAGAAGAGGCAAGCTGAGCTGAAAGCGGGTGATCAGAAAATTGATGAACTTATAAAGGTTTTGGATCAAAGGAAAGATGAATCAATAGAACGTACTTTTAAAGGTGTTGCTAGGCACTTCCGAGAAGTTTTCTCAGAACTTGTGCAAGGTGGCCATGGGCACCTTGTTATGATGAAGAAAAAGGATGGTCAGCATGGTGATGATGATCAGGATGAGGATGGACCACGTGAAGCGGATTTGGAGGGAAGAGTTGAAAAATATATTGGTGTAAAAGTGAAGGTTTCTTTCACTGGACAAGGGGAGACTCAGTCCATGAAGCAGTTGTCTGGGGGTCAGAAAACTGTTGTTGCTCTGACGCTTATTTTTGCCATCCAGCGATGTGATCCTGCCCCATTTTATCTTTTTGATGAAATAGATGCAGCACTGGATCCTCAGTACAGAACCGCTGTTGGAAACATGATCCGACGCCTGGCAGACATAGCAAACACTCAATTTATAACCACAACATTCCGGCCAGAGCTTGTGAAAGTTTCTGACAAGATATATGCTGTTGAACATAAAAACAGGGTGAGCCGTGTCAACGTTGCATCAAAGGACGATGCATTGGACTTCATAGAGCGTGACCAGTCTCACAATGCGGAGTCTTTTGAGTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0000793 GO:0000819 GO:0003674 GO:0003682 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005856 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0008150 GO:0008152 GO:0008278 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010468 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016363 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0022402 GO:0030054 GO:0031974 GO:0031981 GO:0032991 GO:0034399 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051276 GO:0055044 GO:0060147 GO:0060255 GO:0060966 GO:0060968 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0098813 GO:0140096 GO:1900368 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114

Pfam Domains

Protein Families

SMC_N SMC_hinge

Protein Analysis

1272

Amino Acids

146.74

Weight (kDa)

6.26

Isoelectric Point (pI)

44.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_15	PF13175	1 - 461	2.8e-07	AAA ATPase domain
SMC_N	PF02463	2 - 1242	1.1e-64	RecF/RecN/SMC N terminal domain
AAA_23	PF13476	6 - 346	8.1e-07	AAA domain
AAA_21	PF13304	27 - 56	9.6e-06	AAA domain, putative AbiEii toxin, Type IV TA system
SMC_hinge	PF06470	531 - 633	1.4e-22	SMC proteins Flexible Hinge Domain
AAA_21	PF13304	1111 - 1222	6.8e-07	AAA domain, putative AbiEii toxin, Type IV TA system

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001925)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27170 AT2G27170 AT2G27170 AT2G27170
fragaria_vesca	FvH4_5g11061 FvH4_5g11061 FvH4_5g11061 FvH4_5g11061 FvH4_5g11061 FvH4_5g11061
malus_domestica	MD03G1161700.v1.1 MD11G1177700.v1.1
prunus_persica	Prupe.6G149900_v2.0.a1 Prupe.6G149900_v2.0.a1
pyrus_communis	pycom03g11460 pycom03g11470 pycom03g11480 pycom11g15150 pycom11g15160 pycom11g15170
rosa_chinensis	RchiOBHm_Chr1g0362081 RchiOBHm_Chr7g0186721 RchiOBHm_Chr7g0186731
rosa_laevigata	RLG00000004823 RLG00000027673
rosa_multiflora	Rmu_co8477933.1_g000001 Rmu_sc0010614.1_g000002 Rmu_sc0017555.1_g000001 Rmu_sc0018615.1_g000001
rosa_roxburghii	Rroxscaffold_3G00267880 Rroxscaffold_4G00294520
rosa_rugosa	Rorug01G0293400 Rorug01G0293500 Rorug06G0475900 Rorug06G0476000 Rorug06G0476100
rosa_samantha	Rh1AG303600 Rh1BG266900 Rh1CG285000 Rh1DG296900 Rh7AG064800 Rh7BG081000 Rh7CG082800 Rh7DG083900
rosa_wichuraiana	Rw1G027040 Rw7G007200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	2724, 3212, 3656
AasI	GACNNNNNNGTC	1 cut(s)	1319
Acc36I	ACCTGC	1 cut(s)	4
AccB1I	GGYRCC	4 cut(s)	95, 210, 1725, 3318
AccB7I	CCANNNNNTGG	1 cut(s)	727
AccI	GTMKAC	1 cut(s)	1323
AccII	CGCG	1 cut(s)	1116
AciI	CCGC	8 cut(s)	670, 2441, 2573, 2674, 3185, 3392, 3603, 3797
AclI	AACGTT	1 cut(s)	3741
AcoI	YGGCCR	3 cut(s)	2481, 3310, 3671
AcsI	RAATTY	6 cut(s)	367, 1237, 1555, 1807, 1841, 3133
AcuI	CTGAAG	4 cut(s)	52, 692, 911, 2888
AcvI	CACGTG	1 cut(s)	3386
AcyI	GRCGYC	2 cut(s)	514, 3625
AdeI	CACNNNGTG	1 cut(s)	1687
AfaI	GTAC	7 cut(s)	5, 144, 493, 596, 1139, 3253, 3596
AfeI	AGCGCT	1 cut(s)	3063
AfiI	CCNNNNNNNGG	5 cut(s)	727, 1446, 1773, 1774, 3628
AflII	CTTAAG	2 cut(s)	1043, 2363
AjnI	CCWGG	2 cut(s)	819, 3627
AjuI	GAANNNNNNNTTGG	2 cut(s)	3405, 3437
Alw21I	GWGCWC	2 cut(s)	291, 3159
Alw26I	GTCTC	7 cut(s)	1338, 2444, 2612, 2639, 2705, 3455, 3792
Alw44I	GTGCAC	1 cut(s)	287
AlwNI	CAGNNNCTG	1 cut(s)	1009
Ama87I	CYCGRG	2 cut(s)	495, 972
Aor51HI	AGCGCT	1 cut(s)	3063
AoxI	GGCC	5 cut(s)	63, 2481, 2691, 3310, 3671
ApaLI	GTGCAC	1 cut(s)	287
ApeKI	GCWGC	8 cut(s)	418, 667, 1000, 1123, 1178, 2794, 3064, 3578
ApoI	RAATTY	6 cut(s)	367, 1237, 1555, 1807, 1841, 3133
ArsI	GACNNNNNNTTYG	2 cut(s)	1135, 1167
Asp700I	GAANNNNTTC	2 cut(s)	122, 1224
AspLEI	GCGC	3 cut(s)	918, 1434, 3064
AspS9I	GGNCC	5 cut(s)	817, 1604, 1958, 2999, 3380
AsuC2I	CCSGG	5 cut(s)	269, 973, 974, 1533, 1862
AsuHPI	GGTGA	7 cut(s)	1547, 1789, 2069, 2741, 3200, 3368, 3739
AsuII	TTCGAA	1 cut(s)	169
AvaI	CYCGRG	2 cut(s)	495, 972
AvaII	GGWCC	5 cut(s)	817, 1604, 1958, 2999, 3380
BaeGI	GKGCMC	5 cut(s)	213, 291, 2338, 2507, 3321
BaeI	ACNNNNGTAYC	2 cut(s)	483, 516
BalI	TGGCCA	2 cut(s)	2483, 3312
BamHI	GGATCC	1 cut(s)	3586
BanI	GGYRCC	4 cut(s)	95, 210, 1725, 3318
BanII	GRGCYC	1 cut(s)	3159
BauI	CACGAG	1 cut(s)	1182
BbrPI	CACGTG	1 cut(s)	3386
BbsI	GAAGAC	1 cut(s)	2889
Bbv12I	GWGCWC	2 cut(s)	291, 3159
BbvI	GCAGC	8 cut(s)	430, 679, 987, 1135, 1165, 2806, 3051, 3590
BccI	CCATC	8 cut(s)	329, 1075, 1379, 1922, 2024, 3338, 3371, 3536
BceAI	ACGGC	3 cut(s)	1307, 1634, 3717
BciT130I	CCWGG	2 cut(s)	821, 3629
BclI	TGATCA	5 cut(s)	1519, 2518, 3121, 3190, 3364
BcnI	CCSGG	5 cut(s)	269, 973, 974, 1533, 1862
BcoDI	GTCTC	7 cut(s)	1338, 2444, 2612, 2639, 2705, 3455, 3792
BfaI	CTAG	6 cut(s)	374, 747, 2036, 2687, 2924, 3270
BfmI	CTRYAG	5 cut(s)	40, 1001, 1010, 1573, 2817
BfoI	RGCGCY	1 cut(s)	3065
BfrI	CTTAAG	2 cut(s)	1043, 2363
BfuAI	ACCTGC	1 cut(s)	4
BglI	GCCNNNNNGGC	1 cut(s)	2480
BglII	AGATCT	2 cut(s)	1569, 2509
BisI	GCNGC	8 cut(s)	419, 668, 1001, 1124, 1179, 2795, 3065, 3579
BlpI	GCTNAGC	2 cut(s)	1127, 3174
BlsI	GCNGC	8 cut(s)	420, 669, 1002, 1125, 1180, 2796, 3066, 3580
BmcAI	AGTACT	1 cut(s)	596
Bme1390I	CCNGG	7 cut(s)	269, 821, 973, 974, 1533, 1862, 3629
Bme18I	GGWCC	5 cut(s)	817, 1604, 1958, 2999, 3380
BmeT110I	CYCGRG	2 cut(s)	495, 972
BmgT120I	GGNCC	5 cut(s)	817, 1604, 1958, 2999, 3380
BmiI	GGNNCC	8 cut(s)	97, 212, 1196, 1727, 1785, 1821, 3320, 3588
BmrFI	CCNGG	7 cut(s)	269, 821, 973, 974, 1533, 1862, 3629
BmrI	ACTGGG	1 cut(s)	1156
BmuI	ACTGGG	1 cut(s)	1156
BpiI	GAAGAC	1 cut(s)	2889
BplI	GAGNNNNNCTC	2 cut(s)	3141, 3173
BpmI	CTGGAG	5 cut(s)	1273, 2201, 2514, 2663, 3143
Bpu1102I	GCTNAGC	2 cut(s)	1127, 3174
Bpu14I	TTCGAA	1 cut(s)	169
BpuEI	CTTGAG	3 cut(s)	2536, 2687, 2825
BpuMI	CCSGG	5 cut(s)	269, 973, 974, 1533, 1862
BsaAI	YACGTR	2 cut(s)	1592, 3386
BsaBI	GATNNNNATC	1 cut(s)	3363
BsaHI	GRCGYC	2 cut(s)	514, 3625
BsaJI	CCNNGG	5 cut(s)	972, 1787, 1860, 1918, 3313
BsaWI	WCCGGW	1 cut(s)	135
BsaXI	ACNNNNNCTCC	2 cut(s)	3452, 3482
Bsc4I	CCNNNNNNNGG	5 cut(s)	727, 1446, 1773, 1774, 3628
Bse1I	ACTGG	8 cut(s)	550, 967, 1162, 2442, 2475, 3454, 3588, 3784
Bse3DI	GCAATG	2 cut(s)	2373, 3082
Bse8I	GATNNNNATC	1 cut(s)	3363
BseBI	CCWGG	2 cut(s)	821, 3629
BseDI	CCNNGG	5 cut(s)	972, 1787, 1860, 1918, 3313
BseJI	GATNNNNATC	1 cut(s)	3363
BseLI	CCNNNNNNNGG	5 cut(s)	727, 1446, 1773, 1774, 3628
BseMI	GCAATG	2 cut(s)	2373, 3082
BseNI	ACTGG	8 cut(s)	550, 967, 1162, 2442, 2475, 3454, 3588, 3784
BseRI	GAGGAG	4 cut(s)	521, 965, 1558, 2549
BseSI	GKGCMC	5 cut(s)	213, 291, 2338, 2507, 3321
BseXI	GCAGC	8 cut(s)	430, 679, 987, 1135, 1165, 2806, 3051, 3590
BseYI	CCCAGC	2 cut(s)	383, 1664
BsgI	GTGCAG	2 cut(s)	225, 437
Bsh1236I	CGCG	1 cut(s)	1116
BshFI	GGCC	5 cut(s)	65, 2483, 2693, 3312, 3673
BshNI	GGYRCC	4 cut(s)	95, 210, 1725, 3318
BsiHKAI	GWGCWC	2 cut(s)	291, 3159
BsiHKCI	CYCGRG	2 cut(s)	495, 972
BsiSI	CCGG	6 cut(s)	136, 269, 973, 1532, 1862, 3670
BslFI	GGGAC	2 cut(s)	1423, 1427
BslI	CCNNNNNNNGG	5 cut(s)	727, 1446, 1773, 1774, 3628
BsmAI	GTCTC	7 cut(s)	1338, 2444, 2612, 2639, 2705, 3455, 3792
BsmFI	GGGAC	2 cut(s)	1423, 1427
BsnI	GGCC	5 cut(s)	65, 2483, 2693, 3312, 3673
BsoBI	CYCGRG	2 cut(s)	495, 972
Bsp119I	TTCGAA	1 cut(s)	169
Bsp1286I	GDGCHC	6 cut(s)	213, 291, 2338, 2507, 3159, 3321
Bsp1407I	TGTACA	1 cut(s)	3
Bsp1720I	GCTNAGC	2 cut(s)	1127, 3174
Bsp19I	CCATGG	1 cut(s)	3313
BspACI	CCGC	8 cut(s)	670, 2441, 2573, 2674, 3185, 3392, 3603, 3797
BspANI	GGCC	5 cut(s)	65, 2483, 2693, 3312, 3673
BspFNI	CGCG	1 cut(s)	1116
BspLI	GGNNCC	8 cut(s)	97, 212, 1196, 1727, 1785, 1821, 3320, 3588
BspMAI	CTGCAG	3 cut(s)	1005, 1014, 1577
BspMI	ACCTGC	1 cut(s)	4
BspQI	GCTCTTC	2 cut(s)	2169, 3147
BspT104I	TTCGAA	1 cut(s)	169
BspT107I	GGYRCC	4 cut(s)	95, 210, 1725, 3318
BspTI	CTTAAG	2 cut(s)	1043, 2363
BsrDI	GCAATG	2 cut(s)	2373, 3082
BsrGI	TGTACA	1 cut(s)	3
BsrI	ACTGG	8 cut(s)	550, 967, 1162, 2442, 2475, 3454, 3588, 3784
BssECI	CCNNGG	5 cut(s)	972, 1787, 1860, 1918, 3313
BssNI	GRCGYC	2 cut(s)	514, 3625
BssSI	CACGAG	1 cut(s)	1182
BssT1I	CCWWGG	2 cut(s)	1918, 3313
Bst2BI	CACGAG	1 cut(s)	1182
Bst2UI	CCWGG	2 cut(s)	821, 3629
Bst4CI	ACNGT	8 cut(s)	648, 790, 1137, 1984, 2112, 2934, 3093, 3502
Bst6I	CTCTTC	8 cut(s)	621, 2169, 2272, 2390, 2483, 3147, 3158, 3400
BstACI	GRCGYC	2 cut(s)	514, 3625
BstAFI	CTTAAG	2 cut(s)	1043, 2363
BstAPI	GCANNNNNTGC	1 cut(s)	1009
BstAUI	TGTACA	1 cut(s)	3
BstBAI	YACGTR	2 cut(s)	1592, 3386
BstBI	TTCGAA	1 cut(s)	169
BstC8I	GCNNGC	4 cut(s)	186, 718, 2334, 3171
BstDSI	CCRYGG	2 cut(s)	1787, 3313
BstEII	GGTNACC	1 cut(s)	2057
BstFNI	CGCG	1 cut(s)	1116
BstH2I	RGCGCY	1 cut(s)	3065
BstHHI	GCGC	3 cut(s)	918, 1434, 3064
BstMAI	GTCTC	7 cut(s)	1338, 2444, 2612, 2639, 2705, 3455, 3792
BstNI	CCWGG	2 cut(s)	821, 3629
BstNSI	RCATGY	1 cut(s)	188
BstPI	GGTNACC	1 cut(s)	2057
BstSCI	CCNGG	7 cut(s)	267, 819, 971, 972, 1531, 1860, 3627
BstSFI	CTRYAG	5 cut(s)	40, 1001, 1010, 1573, 2817
BstSLI	GKGCMC	5 cut(s)	213, 291, 2338, 2507, 3321
BstUI	CGCG	1 cut(s)	1116
BstV1I	GCAGC	8 cut(s)	430, 679, 987, 1135, 1165, 2806, 3051, 3590
BstV2I	GAAGAC	1 cut(s)	2889
BstX2I	RGATCY	8 cut(s)	104, 838, 1569, 1931, 2509, 2542, 2575, 3586
BstYI	RGATCY	8 cut(s)	104, 838, 1569, 1931, 2509, 2542, 2575, 3586
BsuRI	GGCC	5 cut(s)	65, 2483, 2693, 3312, 3673
BtgI	CCRYGG	2 cut(s)	1787, 3313
BtgZI	GCGATG	1 cut(s)	3550
BtsIMutI	CAGTG	6 cut(s)	443, 795, 2435, 2482, 3447, 3581
BveI	ACCTGC	1 cut(s)	4
Cac8I	GCNNGC	4 cut(s)	186, 718, 2334, 3171
CaiI	CAGNNNCTG	1 cut(s)	1009
CfoI	GCGC	3 cut(s)	918, 1434, 3064
Cfr13I	GGNCC	5 cut(s)	817, 1604, 1958, 2999, 3380
Cfr9I	CCCGGG	1 cut(s)	972
CseI	GACGC	3 cut(s)	503, 3523, 3633
Csp6I	GTAC	7 cut(s)	4, 143, 492, 595, 1138, 3252, 3595
CspCI	CAANNNNNGTGG	6 cut(s)	2059, 2094, 2398, 2433, 2647, 2682
CviQI	GTAC	7 cut(s)	4, 143, 492, 595, 1138, 3252, 3595
DraI	TTTAAA	2 cut(s)	864, 3259
DraIII	CACNNNGTG	1 cut(s)	1687
DrdI	GACNNNNNNGTC	1 cut(s)	1319
DseDI	GACNNNNNNGTC	1 cut(s)	1319
EaeI	YGGCCR	3 cut(s)	2481, 3310, 3671
Eam1104I	CTCTTC	8 cut(s)	621, 2169, 2272, 2390, 2483, 3147, 3158, 3400
EarI	CTCTTC	8 cut(s)	621, 2169, 2272, 2390, 2483, 3147, 3158, 3400
EciI	GGCGGA	2 cut(s)	2456, 2689
Ecl136II	GAGCTC	1 cut(s)	3157
Eco130I	CCWWGG	2 cut(s)	1918, 3313
Eco147I	AGGCCT	1 cut(s)	65
Eco24I	GRGCYC	1 cut(s)	3159
Eco32I	GATATC	1 cut(s)	2209
Eco47I	GGWCC	5 cut(s)	817, 1604, 1958, 2999, 3380
Eco47III	AGCGCT	1 cut(s)	3063
Eco53kI	GAGCTC	1 cut(s)	3157
Eco57I	CTGAAG	4 cut(s)	52, 692, 911, 2888
Eco72I	CACGTG	1 cut(s)	3386
Eco88I	CYCGRG	2 cut(s)	495, 972
Eco91I	GGTNACC	1 cut(s)	2057
EcoICRI	GAGCTC	1 cut(s)	3157
EcoO109I	RGGNCCY	1 cut(s)	817
EcoO65I	GGTNACC	1 cut(s)	2057
EcoRI	GAATTC	1 cut(s)	1807
EcoRII	CCWGG	2 cut(s)	819, 3627
EcoRV	GATATC	1 cut(s)	2209
EcoT14I	CCWWGG	2 cut(s)	1918, 3313
EcoT22I	ATGCAT	2 cut(s)	2133, 3763
EcoT38I	GRGCYC	1 cut(s)	3159
ErhI	CCWWGG	2 cut(s)	1918, 3313
FalI	AAGNNNNNCTT	8 cut(s)	104, 136, 1945, 1977, 2196, 2228, 2574, 2606
FaqI	GGGAC	2 cut(s)	1423, 1427
FauI	CCCGC	1 cut(s)	3178
FbaI	TGATCA	5 cut(s)	1519, 2518, 3121, 3190, 3364
FblI	GTMKAC	1 cut(s)	1323
Fnu4HI	GCNGC	8 cut(s)	419, 668, 1001, 1124, 1179, 2795, 3065, 3579
FriOI	GRGCYC	1 cut(s)	3159
Fsp4HI	GCNGC	8 cut(s)	419, 668, 1001, 1124, 1179, 2795, 3065, 3579
FspBI	CTAG	6 cut(s)	374, 747, 2036, 2687, 2924, 3270
GlaI	GCGC	3 cut(s)	917, 1433, 3063
GluI	GCNGC	8 cut(s)	419, 668, 1001, 1124, 1179, 2795, 3065, 3579
GsaI	CCCAGC	2 cut(s)	387, 1668
GsuI	CTGGAG	5 cut(s)	1273, 2201, 2514, 2663, 3143
HaeII	RGCGCY	1 cut(s)	3065
HaeIII	GGCC	5 cut(s)	65, 2483, 2693, 3312, 3673
HapII	CCGG	6 cut(s)	136, 269, 973, 1532, 1862, 3670
HgaI	GACGC	3 cut(s)	503, 3523, 3633
HhaI	GCGC	3 cut(s)	918, 1434, 3064
Hin1I	GRCGYC	2 cut(s)	514, 3625
Hin6I	GCGC	3 cut(s)	916, 1432, 3062
HinP1I	GCGC	3 cut(s)	916, 1432, 3062
HincII	GTYRAC	3 cut(s)	940, 3106, 3739
HindII	GTYRAC	3 cut(s)	940, 3106, 3739
HindIII	AAGCTT	3 cut(s)	858, 2412, 2588
HpaII	CCGG	6 cut(s)	136, 269, 973, 1532, 1862, 3670
HphI	GGTGA	7 cut(s)	1547, 1789, 2069, 2741, 3200, 3368, 3739
Hpy166II	GTNNAC	7 cut(s)	289, 940, 1324, 1748, 1958, 3106, 3739
Hpy8I	GTNNAC	7 cut(s)	289, 940, 1324, 1748, 1958, 3106, 3739
Hpy99I	CGWCG	2 cut(s)	519, 3627
HpyCH4III	ACNGT	8 cut(s)	648, 790, 1137, 1984, 2112, 2934, 3093, 3502
HpyCH4IV	ACGT	7 cut(s)	463, 1326, 1591, 1683, 3250, 3385, 3741
HpySE526I	ACGT	7 cut(s)	463, 1326, 1591, 1683, 3250, 3385, 3741
Hsp92I	GRCGYC	2 cut(s)	514, 3625
HspAI	GCGC	3 cut(s)	916, 1432, 3062
Ksp22I	TGATCA	5 cut(s)	1519, 2518, 3121, 3190, 3364
LguI	GCTCTTC	2 cut(s)	2169, 3147
LmnI	GCTCC	5 cut(s)	401, 1200, 1789, 1852, 3162
Lsp1109I	GCAGC	8 cut(s)	430, 679, 987, 1135, 1165, 2806, 3051, 3590
MaeI	CTAG	6 cut(s)	374, 747, 2036, 2687, 2924, 3270
MaeII	ACGT	7 cut(s)	463, 1326, 1591, 1683, 3250, 3385, 3741
MfeI	CAATTG	1 cut(s)	2245
MflI	RGATCY	8 cut(s)	104, 838, 1569, 1931, 2509, 2542, 2575, 3586
MhlI	GDGCHC	6 cut(s)	213, 291, 2338, 2507, 3159, 3321
MlsI	TGGCCA	2 cut(s)	2483, 3312
MluNI	TGGCCA	2 cut(s)	2483, 3312
MlyI	GAGTC	8 cut(s)	449, 1015, 1340, 2407, 2446, 2740, 3457, 3809
MmeI	TCCRAC	3 cut(s)	600, 3589, 3646
Mox20I	TGGCCA	2 cut(s)	2483, 3312
Mph1103I	ATGCAT	2 cut(s)	2133, 3763
MroXI	GAANNNNTTC	2 cut(s)	122, 1224
MscI	TGGCCA	2 cut(s)	2483, 3312
MslI	CAYNNNNRTG	2 cut(s)	201, 3357
Msp20I	TGGCCA	2 cut(s)	2483, 3312
MspA1I	CMGCKG	3 cut(s)	670, 1000, 3605
MspCI	CTTAAG	2 cut(s)	1043, 2363
MspI	CCGG	6 cut(s)	136, 269, 973, 1532, 1862, 3670
MspR9I	CCNGG	7 cut(s)	269, 821, 973, 974, 1533, 1862, 3629
MunI	CAATTG	1 cut(s)	2245
MvaI	CCWGG	2 cut(s)	821, 3629
MvnI	CGCG	1 cut(s)	1116
NciI	CCSGG	5 cut(s)	269, 973, 974, 1533, 1862
NcoI	CCATGG	1 cut(s)	3313
NlaIV	GGNNCC	8 cut(s)	97, 212, 1196, 1727, 1785, 1821, 3320, 3588
NmuCI	GTSAC	7 cut(s)	149, 1184, 1310, 1377, 1628, 2057, 3779
NsiI	ATGCAT	2 cut(s)	2133, 3763
NspI	RCATGY	1 cut(s)	188
NspV	TTCGAA	1 cut(s)	169
PaeI	GCATGC	1 cut(s)	188
PaeR7I	CTCGAG	1 cut(s)	495
PceI	AGGCCT	1 cut(s)	65
PciSI	GCTCTTC	2 cut(s)	2169, 3147
PdmI	GAANNNNTTC	2 cut(s)	122, 1224
PfeI	GAWTC	5 cut(s)	264, 1028, 1361, 1876, 3239
PflFI	GACNNNGTC	2 cut(s)	2063, 3466
PflMI	CCANNNNNTGG	1 cut(s)	727
PkrI	GCNGC	8 cut(s)	420, 669, 1002, 1125, 1180, 2796, 3066, 3580
PleI	GAGTC	8 cut(s)	449, 1014, 1339, 2406, 2446, 2740, 3457, 3808
PmaCI	CACGTG	1 cut(s)	3386
PmlI	CACGTG	1 cut(s)	3386
PpsI	GAGTC	8 cut(s)	449, 1014, 1339, 2406, 2446, 2740, 3457, 3808
Ppu21I	YACGTR	2 cut(s)	1592, 3386
PpuMI	RGGWCCY	1 cut(s)	817
PsiI	TTATAA	3 cut(s)	2724, 3212, 3656
Psp124BI	GAGCTC	1 cut(s)	3159
Psp1406I	AACGTT	1 cut(s)	3741
Psp5II	RGGWCCY	1 cut(s)	817
Psp6I	CCWGG	2 cut(s)	819, 3627
PspCI	CACGTG	1 cut(s)	3386
PspEI	GGTNACC	1 cut(s)	2057
PspFI	CCCAGC	2 cut(s)	383, 1664
PspGI	CCWGG	2 cut(s)	819, 3627
PspN4I	GGNNCC	8 cut(s)	97, 212, 1196, 1727, 1785, 1821, 3320, 3588
PspPI	GGNCC	5 cut(s)	817, 1604, 1958, 2999, 3380
PspPPI	RGGWCCY	1 cut(s)	817
PspXI	VCTCGAGB	1 cut(s)	495
PstI	CTGCAG	3 cut(s)	1005, 1014, 1577
PstNI	CAGNNNCTG	1 cut(s)	1009
PsuI	RGATCY	8 cut(s)	104, 838, 1569, 1931, 2509, 2542, 2575, 3586
PsyI	GACNNNGTC	2 cut(s)	2063, 3466
PvuII	CAGCTG	1 cut(s)	1000
RsaI	GTAC	7 cut(s)	5, 144, 493, 596, 1139, 3253, 3596
RsaNI	GTAC	7 cut(s)	4, 143, 492, 595, 1138, 3252, 3595
RseI	CAYNNNNRTG	2 cut(s)	201, 3357
SacI	GAGCTC	1 cut(s)	3159
SapI	GCTCTTC	2 cut(s)	2169, 3147
SatI	GCNGC	8 cut(s)	419, 668, 1001, 1124, 1179, 2795, 3065, 3579
Sau96I	GGNCC	5 cut(s)	817, 1604, 1958, 2999, 3380
ScaI	AGTACT	1 cut(s)	596
SchI	GAGTC	8 cut(s)	449, 1015, 1340, 2407, 2446, 2740, 3457, 3809
ScrFI	CCNGG	7 cut(s)	269, 821, 973, 974, 1533, 1862, 3629
SduI	GDGCHC	6 cut(s)	213, 291, 2338, 2507, 3159, 3321
SfcI	CTRYAG	5 cut(s)	40, 1001, 1010, 1573, 2817
Sfr274I	CTCGAG	1 cut(s)	495
SfuI	TTCGAA	1 cut(s)	169
SinI	GGWCC	5 cut(s)	817, 1604, 1958, 2999, 3380
SlaI	CTCGAG	1 cut(s)	495
SmaI	CCCGGG	1 cut(s)	974
SmiMI	CAYNNNNRTG	2 cut(s)	201, 3357
SmlI	CTYRAG	6 cut(s)	495, 1043, 2363, 2551, 2666, 2840
SmoI	CTYRAG	6 cut(s)	495, 1043, 2363, 2551, 2666, 2840
SphI	GCATGC	1 cut(s)	188
SseBI	AGGCCT	1 cut(s)	65
SsiI	CCGC	8 cut(s)	670, 2441, 2573, 2674, 3185, 3392, 3603, 3797
SspMI	CTAG	6 cut(s)	374, 747, 2036, 2687, 2924, 3270
SstI	GAGCTC	1 cut(s)	3159
StuI	AGGCCT	1 cut(s)	65
StyD4I	CCNGG	7 cut(s)	267, 819, 971, 972, 1531, 1860, 3627
StyI	CCWWGG	2 cut(s)	1918, 3313
TaaI	ACNGT	8 cut(s)	648, 790, 1137, 1984, 2112, 2934, 3093, 3502
TaiI	ACGT	7 cut(s)	466, 1329, 1594, 1686, 3253, 3388, 3744
TaqI	TCGA	6 cut(s)	169, 262, 496, 517, 1336, 1993
TatI	WGTACW	4 cut(s)	3, 142, 594, 3594
TfiI	GAWTC	5 cut(s)	264, 1028, 1361, 1876, 3239
TscAI	CASTG	6 cut(s)	450, 795, 2442, 2482, 3454, 3588
TseFI	GTSAC	7 cut(s)	149, 1184, 1310, 1377, 1628, 2057, 3779
TseI	GCWGC	8 cut(s)	418, 667, 1000, 1123, 1178, 2794, 3064, 3578
Tsp45I	GTSAC	7 cut(s)	149, 1184, 1310, 1377, 1628, 2057, 3779
TspGWI	ACGGA	2 cut(s)	371, 2621
TspMI	CCCGGG	1 cut(s)	972
TspRI	CASTG	6 cut(s)	450, 795, 2442, 2482, 3454, 3588
Tth111I	GACNNNGTC	2 cut(s)	2063, 3466
Van91I	CCANNNNNTGG	1 cut(s)	727
Vha464I	CTTAAG	2 cut(s)	1043, 2363
VneI	GTGCAC	1 cut(s)	287
VpaK11BI	GGWCC	5 cut(s)	817, 1604, 1958, 2999, 3380
XapI	RAATTY	6 cut(s)	367, 1237, 1555, 1807, 1841, 3133
XbaI	TCTAGA	1 cut(s)	2035
XceI	RCATGY	1 cut(s)	188
XhoI	CTCGAG	1 cut(s)	495
XmaI	CCCGGG	1 cut(s)	972
XmiI	GTMKAC	1 cut(s)	1323
XmnI	GAANNNNTTC	2 cut(s)	122, 1224
XspI	CTAG	6 cut(s)	374, 747, 2036, 2687, 2924, 3270
ZrmI	AGTACT	1 cut(s)	596
Zsp2I	ATGCAT	2 cut(s)	2133, 3763

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.