Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0001925

Physical Location & Seq

Genomic Coordinates Forward (+)

62817660 .. 62829760

Gene Structure 12101 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004823

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3552 bp

ATGCACATAAAGCAGATTATAATTGAAGGATTTAAGAGCTACAGAGAACAGGTTGCTACTGAGCCATTCAGTCCAAAAATCAATTGCGTTGTTGGTGCTAATGGGTCTGGAAAGACAAACTTTTTCCATGCAATTCGGTTTGTACTAAGTGACCTTTTCCAAAACCTTCGAAGTGACGATCGGCATGCTCTACTCCATGAAGGTGCAGGGCACCAAGTCTTATCTGCATTTGTGGAGATTGTCTTCGACAACTCTGACAATCGAATACCGGTTGATAAGGAGGAAGTGCGGTTGCGAAGGACAATTGGTTTGAAGAAGGATGAGTATTTCTTGGATGGAAAGCACATCACGAAAACTGAGGTTATGAATTTACTGGAAAGTGCTGGGTTCTCTCGCTCCAATCCATATTATGTTGTGCAGCAAGGAAAGATAGCATCCTTGACACTGATGAAAGATTCTGAGCGACTGGATTTACTTAAGGAAATCGGTGGCACTCGAGTGTATGAGGAGAGACGTCAAGAAAGTTTGAAAATCATGCAGGACACTGGTAACAAAAGAAAGCAGATAATTCAAGTTGTTCAGTACTTGGATGAGAGATTAAAGGAACTGGATGAAGAGAAAGAGGAACTTAGAAAGTATCAGCAGCTTGACAAGCAGCGAAAATCTCTGGAATACACTATATATGACAAGGAGCTTCAGGATGCTCGCCAAAGGCTGGCAGAGGTAGAAGATGATCGGAATAAGGTTTCTGAAACATCGACGAGGATGTATAATAGTGTGCTGGATGCCCATGAAAAGTCCAAGGACTTGGATAAGGCTTTGAAAGATCTGACAAAAGAGCTACAAGCTTTAAATAAAGAGAAAGAAGCAGTGGAAAAACGAAGAACAGAAGCGATAAAGAAACACACAGAGCTTGAGCTTGATGTCAAAGATCTACAGGAGAAGATATCTGGAAACATCCGAGCCAAAGAAGATGCTGTGAAACAACTACAGAGTCTGCAGAAAGAGATTCAGGATTCAATGGATGAGCTTGAGAATATAAGTCCTCTGTATGAGAATCAAGTCATGAGGGAAAAGGAGATAACAAAGGGGTATGAGAACAAGCTTCAGGATGAAATTAAACGTCTTAATGCTGAGTTGAACGAGCGGGGTACTTACATTGAATCTCGAAGAAATGAAATTACTAACAAGGAATCTCTCATCTCTCAGTCACGTGAAGGGTTCAGTCATCATAAAGCAGAGAGGGACAAGATGCAGGATGAGCGCAAGACCCTATGGAGGAAAGAAACAGAACTTTCTGCTGAAATTGATAAACTGAGAACAGAAGTTGAGAAGGCAGAGAAGAGCCTTGATCATTCAACTCCTGGTGATGTGAGGAGAGGGCTAAATTCTGTTCGTAAGATCTGCAGAGAGTATAATATTCCTGGGGTATTTGGTCCTATTATTGAGTTGCTTGATTGTGAGGAAAAGTTTTTTACAGCAGTGGAAGTTACTGCTGGGAACAGCTTATTTCATGTTGTGGTTGAAAATGATGAAATATCAACTCAGATAATCAGGCACCTTAATTCACTGAAAGGTGGGCGTGTTACATTTATCCCCCTGAATAGGGTGAGGGCTCCAAGGGTAACTTATCCACAAAACTCAGATGTGGTTCCTTTGTTGAAGAGGTTAAAGTTTCTTCCAAAGTACACTGCTGCATTTGCTCAGGTATTTGCTAGAACAGTTGTTTGTCGAGATCTGGATGTGGCCACAAAGGTTGCTCGTAGCGATGGTCTAGATTGCATCACTTTAGAAGGAGATCAAGTCAGCAAGAAGGGTGGGATGACGGGAGGATTTTATGATCACAGACGCTCAAAGTTGAAGTTCATGAATCTAATCCGACAGAATACAAAATCTATCAGCTTGAAAGAAGAAGAACTTGAGAAAATCAGAAATATGCTTGAAAATATCCTCTCAAAAGTTCTGGACACCCCTACTCTTAACTCAGCTCTGAAAAGCCTTGCGAAGATGGGGTGTTGGGAGATGATAGACGAGCAAATTACAAAACTTGTAACTGAGCAGCAGAAAATTGATGCTGAGCGAGCCCATGTCAAATCAGAACTGGAACAGCTTAAGCAGGACATTGCGAATGCTAATAAGCAGCAAAGTTTAATTTCTAAAGCACTTGGGAATAAGGAAAAATCACTTGCTGATGTGCGGGTTCAAATTGATCAGCTTAGAGCTAGTATGGCCATGAAGCATGCTGAAATGGGCACCGATCTCATTGATCATTTGACTCCAGAGGAGAAGGATCTTCTCTCAAGATTGAACCCTGAAATAGCAGATCTTAAAGAGAAGCTTATTACATGCAAGGCGGATCGAAGTGAGACTGAAGCAAGAAAGGCAGAGCTTGAAACTAATTTAACTACGAACCTTAAGAGGCGGAAGCAAGAACTAGAGGCAATAATATCTTCTGTGGAAACTGATAATTTGCATGGTGAAGATGAAATAAAGATTCAGGAACTGAATGATGCTAGATTATTGGTTGAAGATGCAACAGAGCAGCTCAGAAGAGTTTCTGAAAGTATTGATGGTCAGTCGAGGCAATTGAGGAAAACCAAGGATGAAAAGACTAAGTTGAAGAACTTGGAAGACAACTATGAGAGGACACTTCAGGATGAAGCCAAACAGCTGGAGCAGCTACTGAGTAAAAGAAATATGTATCTTGCTAAACAAGAAGAGTACTCAAAGAAAATCAGGGAATTGGGTGCATTGTCATCGGATGCTTTTGAAACGTATAAGCGAAGGAACATCAAAGAACTACATAAAATGCTGCACAGATGCAATGAGCAACTGCAACAGTTTAGTCATGTCAACAAGAAAGCACTTGACCAGTATGTAAATTTTACAGAACAACGTGAAGAACTCCAGAGAAGGCAAGCTGAACTGGATGCAGGTGATGAGAAAATTGCAGAACTTATATCTGTCTTAGATCAAAGGAAGGATGAATCAATAGAACGCACTTTTAAAGGTGTTGCTAGGAACTTTCGAGAAGTTTTCTCTGAACTTGTGCAAGGTGGCCATGGTCACCTTCTCATGGTGAAAAGAAAGGATGGTAACCATGCTGACGATGATCTGGATGAGGACGATGGACCAGGGGAAACAGATAGATCTGAGAAATATATTGGTGTGAAAGTAAAGGTTTCTTTTACCGGGCAAGGAGAGACTCAGTCCATGAAGCAGTTGTCTGGGGGTCAGAAAACTGTTGTTGCATTGACGCTTATCTTTGCAATACAGCAATGTGATCCTGCTCCTTTCTATCTTTTCGATGAAATTGATGCAGCGCTGGATCCTCAGTACAGGACTGCTGTTGGAAATATGATCCGACGCCTGGCAGATACAGAAACTACTCAATTTATAACTACAACATTCCGACAAGAGCTTGTGAAAGTTTCTGACAAGATATATGGTGTTACACATTCAAACAGGGATAGGAAAAGCAAGGTTCAACAGAACTTTAGAAGGACAATGGAGCAGCCTGCATTCGGGTCTTGTGTCATATTCTTCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0000793 GO:0000819 GO:0003674 GO:0003682 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005856 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0008150 GO:0008152 GO:0008278 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010468 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016363 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0022402 GO:0030054 GO:0031974 GO:0031981 GO:0032991 GO:0034399 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051276 GO:0055044 GO:0060147 GO:0060255 GO:0060966 GO:0060968 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0098813 GO:0140096 GO:1900368 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114

Pfam Domains

Protein Families

SMC_N SMC_hinge

Protein Analysis

1184

Amino Acids

136.54

Weight (kDa)

6.42

Isoelectric Point (pI)

47.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_15	PF13175	1 - 403	3.1e-08	AAA ATPase domain
SMC_N	PF02463	2 - 1156	1.4e-59	RecF/RecN/SMC N terminal domain
AAA_23	PF13476	6 - 310	3.2e-10	AAA domain
AAA_21	PF13304	27 - 56	5.5e-06	AAA domain, putative AbiEii toxin, Type IV TA system
SMC_hinge	PF06470	475 - 587	4.2e-28	SMC proteins Flexible Hinge Domain
AAA_21	PF13304	1045 - 1136	3.2e-06	AAA domain, putative AbiEii toxin, Type IV TA system

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001925)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27170 AT2G27170 AT2G27170 AT2G27170
fragaria_vesca	FvH4_5g11061 FvH4_5g11061 FvH4_5g11061 FvH4_5g11061 FvH4_5g11061 FvH4_5g11061
malus_domestica	MD03G1161700.v1.1 MD11G1177700.v1.1
prunus_persica	Prupe.6G149900_v2.0.a1 Prupe.6G149900_v2.0.a1
pyrus_communis	pycom03g11460 pycom03g11470 pycom03g11480 pycom11g15150 pycom11g15160 pycom11g15170
rosa_chinensis	RchiOBHm_Chr1g0362081 RchiOBHm_Chr7g0186721 RchiOBHm_Chr7g0186731
rosa_laevigata	RLG00000004823 RLG00000027673
rosa_multiflora	Rmu_co8477933.1_g000001 Rmu_sc0010614.1_g000002 Rmu_sc0017555.1_g000001 Rmu_sc0018615.1_g000001
rosa_roxburghii	Rroxscaffold_3G00267880 Rroxscaffold_4G00294520
rosa_rugosa	Rorug01G0293400 Rorug01G0293500 Rorug06G0475900 Rorug06G0476000 Rorug06G0476100
rosa_samantha	Rh1AG303600 Rh1BG266900 Rh1CG285000 Rh1DG296900 Rh7AG064800 Rh7BG081000 Rh7CG082800 Rh7DG083900
rosa_wichuraiana	Rw1G027040 Rw7G007200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	20, 3398
AarI	CACCTGC	1 cut(s)	2924
AatII	GACGTC	1 cut(s)	517
Acc36I	ACCTGC	1 cut(s)	2924
AccB1I	GGYRCC	3 cut(s)	210, 1557, 2252
AccB7I	CCANNNNNTGG	1 cut(s)	715
AccBSI	CCGCTC	1 cut(s)	1147
AciI	CCGC	5 cut(s)	289, 1147, 2197, 2354, 2422
AclWI	GGATC	6 cut(s)	2298, 2364, 3278, 3323, 3336, 3355
AcoI	YGGCCR	3 cut(s)	1746, 2229, 3058
AcsI	RAATTY	3 cut(s)	367, 1387, 2881
AcuI	CTGAAG	4 cut(s)	680, 1091, 2391, 2636
AcvI	CACGTG	1 cut(s)	1214
AcyI	GRCGYC	2 cut(s)	514, 3367
AfaI	GTAC	6 cut(s)	144, 584, 1153, 1688, 2723, 3338
AfeI	AGCGCT	1 cut(s)	3324
AfiI	CCNNNNNNNGG	7 cut(s)	715, 1278, 1605, 1606, 3076, 3370, 3524
AflII	CTTAAG	3 cut(s)	476, 2111, 2414
AgeI	ACCGGT	1 cut(s)	268
AjnI	CCWGG	4 cut(s)	1363, 1423, 3133, 3369
AleI	CACNNNNGTG	1 cut(s)	497
Alw26I	GTCTC	3 cut(s)	505, 2360, 3197
AlwI	GGATC	6 cut(s)	2298, 2364, 3278, 3323, 3336, 3355
AlwNI	CAGNNNCTG	3 cut(s)	997, 2503, 2683
Ama87I	CYCGRG	1 cut(s)	495
Aor51HI	AGCGCT	1 cut(s)	3324
AoxI	GGCC	3 cut(s)	1746, 2229, 3058
ApoI	RAATTY	3 cut(s)	367, 1387, 2881
ArsI	GACNNNNNNTTYG	2 cut(s)	292, 324
AsiGI	ACCGGT	1 cut(s)	268
Asp700I	GAANNNNTTC	1 cut(s)	2554
AspLEI	GCGC	2 cut(s)	1266, 3325
AspS9I	GGNCC	2 cut(s)	1436, 3131
AsuC2I	CCSGG	1 cut(s)	3193
AsuHPI	GGTGA	6 cut(s)	1379, 1621, 2489, 2948, 3059, 3091
AsuII	TTCGAA	1 cut(s)	169
AvaI	CYCGRG	1 cut(s)	495
AvaII	GGWCC	2 cut(s)	1436, 3131
BaeGI	GKGCMC	2 cut(s)	213, 2255
BalI	TGGCCA	3 cut(s)	1748, 2231, 3060
BamHI	GGATCC	1 cut(s)	3328
BanI	GGYRCC	3 cut(s)	210, 1557, 2252
BanII	GRGCYC	2 cut(s)	1618, 2086
BbrPI	CACGTG	1 cut(s)	1214
BbsI	GAAGAC	2 cut(s)	235, 2637
BccI	CCATC	6 cut(s)	329, 1763, 2002, 2564, 3086, 3122
BcgI	CGANNNNNNTGC	4 cut(s)	167, 201, 2739, 2773
BciT130I	CCWGG	4 cut(s)	1365, 1425, 3135, 3371
BclI	TGATCA	4 cut(s)	1351, 1840, 2209, 2266
BcnI	CCSGG	1 cut(s)	3193
BcoDI	GTCTC	3 cut(s)	505, 2360, 3197
BfaI	CTAG	6 cut(s)	1716, 1775, 2223, 2435, 2514, 3018
BfmI	CTRYAG	5 cut(s)	40, 935, 989, 998, 1405
BfoI	RGCGCY	1 cut(s)	3326
BfrI	CTTAAG	3 cut(s)	476, 2111, 2414
BfuAI	ACCTGC	1 cut(s)	2924
BglII	AGATCT	6 cut(s)	826, 931, 1401, 1735, 2323, 3149
BlpI	GCTNAGC	1 cut(s)	2076
BmcAI	AGTACT	2 cut(s)	584, 2723
Bme1390I	CCNGG	5 cut(s)	1365, 1425, 3135, 3193, 3371
Bme18I	GGWCC	2 cut(s)	1436, 3131
BmeT110I	CYCGRG	1 cut(s)	495
BmgT120I	GGNCC	2 cut(s)	1436, 3131
BmiI	GGNNCC	6 cut(s)	212, 1559, 1617, 1653, 2254, 3330
BmrFI	CCNGG	5 cut(s)	1365, 1425, 3135, 3193, 3371
BpiI	GAAGAC	2 cut(s)	235, 2637
BpmI	CTGGAG	3 cut(s)	2262, 2693, 2891
Bpu10I	CCTNAGC	1 cut(s)	1704
Bpu1102I	GCTNAGC	1 cut(s)	2076
Bpu14I	TTCGAA	1 cut(s)	169
BpuEI	CTTGAG	4 cut(s)	935, 1052, 1940, 2284
BpuMI	CCSGG	1 cut(s)	3193
BsaAI	YACGTR	1 cut(s)	1214
BsaHI	GRCGYC	2 cut(s)	514, 3367
BsaJI	CCNNGG	6 cut(s)	801, 1424, 1619, 2598, 3061, 3134
BsaWI	WCCGGW	1 cut(s)	268
BsaXI	ACNNNNNCTCC	2 cut(s)	1788, 1818
Bsc4I	CCNNNNNNNGG	7 cut(s)	715, 1278, 1605, 1606, 3076, 3370, 3524
Bse118I	RCCGGY	1 cut(s)	268
Bse1I	ACTGG	7 cut(s)	378, 471, 550, 612, 2106, 2872, 2931
Bse3DI	GCAATG	3 cut(s)	2121, 2830, 3284
BseBI	CCWGG	4 cut(s)	1365, 1425, 3135, 3371
BseDI	CCNNGG	6 cut(s)	801, 1424, 1619, 2598, 3061, 3134
BseLI	CCNNNNNNNGG	7 cut(s)	715, 1278, 1605, 1606, 3076, 3370, 3524
BseMI	GCAATG	3 cut(s)	2121, 2830, 3284
BseNI	ACTGG	7 cut(s)	378, 471, 550, 612, 2106, 2872, 2931
BseRI	GAGGAG	3 cut(s)	521, 1390, 2297
BseSI	GKGCMC	2 cut(s)	213, 2255
BseYI	CCCAGC	2 cut(s)	383, 1496
BsgI	GTGCAG	3 cut(s)	225, 437, 2798
Bsh1285I	CGRYCG	1 cut(s)	181
BshFI	GGCC	3 cut(s)	1748, 2231, 3060
BshNI	GGYRCC	3 cut(s)	210, 1557, 2252
BshTI	ACCGGT	1 cut(s)	268
BsiEI	CGRYCG	1 cut(s)	181
BsiHKCI	CYCGRG	1 cut(s)	495
BsiSI	CCGG	2 cut(s)	269, 3192
BslFI	GGGAC	1 cut(s)	1259
BslI	CCNNNNNNNGG	7 cut(s)	715, 1278, 1605, 1606, 3076, 3370, 3524
BsmAI	GTCTC	3 cut(s)	505, 2360, 3197
BsmBI	CGTCTC	1 cut(s)	505
BsmFI	GGGAC	1 cut(s)	1259
BsmI	GAATGC	2 cut(s)	2134, 3521
BsnI	GGCC	3 cut(s)	1748, 2231, 3060
BsoBI	CYCGRG	1 cut(s)	495
Bsp119I	TTCGAA	1 cut(s)	169
Bsp1286I	GDGCHC	4 cut(s)	213, 1618, 2086, 2255
Bsp1720I	GCTNAGC	1 cut(s)	2076
Bsp19I	CCATGG	1 cut(s)	3061
BspACI	CCGC	5 cut(s)	289, 1147, 2197, 2354, 2422
BspANI	GGCC	3 cut(s)	1748, 2231, 3060
BspHI	TCATGA	2 cut(s)	1065, 1866
BspLI	GGNNCC	6 cut(s)	212, 1559, 1617, 1653, 2254, 3330
BspMAI	CTGCAG	2 cut(s)	1002, 1409
BspMI	ACCTGC	1 cut(s)	2924
BspPI	GGATC	6 cut(s)	2298, 2364, 3278, 3323, 3336, 3355
BspQI	GCTCTTC	1 cut(s)	1337
BspT104I	TTCGAA	1 cut(s)	169
BspT107I	GGYRCC	3 cut(s)	210, 1557, 2252
BspTI	CTTAAG	3 cut(s)	476, 2111, 2414
BsrBI	CCGCTC	1 cut(s)	1147
BsrDI	GCAATG	3 cut(s)	2121, 2830, 3284
BsrFI	RCCGGY	1 cut(s)	268
BsrI	ACTGG	7 cut(s)	378, 471, 550, 612, 2106, 2872, 2931
BssAI	RCCGGY	1 cut(s)	268
BssECI	CCNNGG	6 cut(s)	801, 1424, 1619, 2598, 3061, 3134
BssNI	GRCGYC	2 cut(s)	514, 3367
BssT1I	CCWWGG	4 cut(s)	801, 1619, 2598, 3061
Bst2UI	CCWGG	4 cut(s)	1365, 1425, 3135, 3371
Bst4CI	ACNGT	3 cut(s)	1723, 2841, 3244
Bst6I	CTCTTC	5 cut(s)	609, 1337, 1658, 2545, 2712
BstACI	GRCGYC	2 cut(s)	514, 3367
BstAFI	CTTAAG	3 cut(s)	476, 2111, 2414
BstBAI	YACGTR	1 cut(s)	1214
BstBI	TTCGAA	1 cut(s)	169
BstC8I	GCNNGC	7 cut(s)	186, 706, 717, 2082, 2241, 2919, 3519
BstDSI	CCRYGG	1 cut(s)	3061
BstEII	GGTNACC	2 cut(s)	3065, 3095
BstH2I	RGCGCY	1 cut(s)	3326
BstHHI	GCGC	2 cut(s)	1266, 3325
BstMAI	GTCTC	3 cut(s)	505, 2360, 3197
BstMCI	CGRYCG	1 cut(s)	181
BstMWI	GCNNNNNNNGC	8 cut(s)	10, 652, 1261, 1700, 2081, 2228, 2381, 2677
BstNI	CCWGG	4 cut(s)	1365, 1425, 3135, 3371
BstNSI	RCATGY	3 cut(s)	188, 2243, 2349
BstPI	GGTNACC	2 cut(s)	3065, 3095
BstSCI	CCNGG	5 cut(s)	1363, 1423, 3133, 3191, 3369
BstSFI	CTRYAG	5 cut(s)	40, 935, 989, 998, 1405
BstSLI	GKGCMC	2 cut(s)	213, 2255
BstV2I	GAAGAC	2 cut(s)	235, 2637
BstX2I	RGATCY	8 cut(s)	826, 931, 1401, 1735, 2290, 2323, 3149, 3328
BstXI	CCANNNNNNTGG	2 cut(s)	808, 2671
BstYI	RGATCY	8 cut(s)	826, 931, 1401, 1735, 2290, 2323, 3149, 3328
BsuRI	GGCC	3 cut(s)	1748, 2231, 3060
BtgI	CCRYGG	1 cut(s)	3061
BtgZI	GCGATG	1 cut(s)	1782
BtsI	GCAGTG	3 cut(s)	876, 1488, 1689
BtsIMutI	CAGTG	6 cut(s)	443, 543, 876, 1488, 1568, 1689
BveI	ACCTGC	1 cut(s)	2924
Cac8I	GCNNGC	7 cut(s)	186, 706, 717, 2082, 2241, 2919, 3519
CaiI	CAGNNNCTG	3 cut(s)	997, 2503, 2683
CciI	TCATGA	2 cut(s)	1065, 1866
CfoI	GCGC	2 cut(s)	1266, 3325
Cfr10I	RCCGGY	1 cut(s)	268
Cfr13I	GGNCC	2 cut(s)	1436, 3131
CseI	GACGC	3 cut(s)	1857, 3265, 3375
Csp6I	GTAC	6 cut(s)	143, 583, 1152, 1687, 2722, 3337
CspAI	ACCGGT	1 cut(s)	268
CspCI	CAANNNNNGTGG	4 cut(s)	1738, 1773, 1798, 1833
CviQI	GTAC	6 cut(s)	143, 583, 1152, 1687, 2722, 3337
DraI	TTTAAA	2 cut(s)	852, 3007
EaeI	YGGCCR	3 cut(s)	1746, 2229, 3058
Eam1104I	CTCTTC	5 cut(s)	609, 1337, 1658, 2545, 2712
EarI	CTCTTC	5 cut(s)	609, 1337, 1658, 2545, 2712
EciI	GGCGGA	2 cut(s)	2369, 2437
Eco130I	CCWWGG	4 cut(s)	801, 1619, 2598, 3061
Eco24I	GRGCYC	2 cut(s)	1618, 2086
Eco32I	GATATC	1 cut(s)	948
Eco47I	GGWCC	2 cut(s)	1436, 3131
Eco47III	AGCGCT	1 cut(s)	3324
Eco57I	CTGAAG	4 cut(s)	680, 1091, 2391, 2636
Eco72I	CACGTG	1 cut(s)	1214
Eco88I	CYCGRG	1 cut(s)	495
Eco91I	GGTNACC	2 cut(s)	3065, 3095
EcoO65I	GGTNACC	2 cut(s)	3065, 3095
EcoRII	CCWGG	4 cut(s)	1363, 1423, 3133, 3369
EcoRV	GATATC	1 cut(s)	948
EcoT14I	CCWWGG	4 cut(s)	801, 1619, 2598, 3061
EcoT38I	GRGCYC	2 cut(s)	1618, 2086
ErhI	CCWWGG	4 cut(s)	801, 1619, 2598, 3061
Esp3I	CGTCTC	1 cut(s)	505
FaqI	GGGAC	1 cut(s)	1259
FauI	CCCGC	2 cut(s)	1140, 2190
FbaI	TGATCA	4 cut(s)	1351, 1840, 2209, 2266
FriOI	GRGCYC	2 cut(s)	1618, 2086
FspBI	CTAG	6 cut(s)	1716, 1775, 2223, 2435, 2514, 3018
GlaI	GCGC	2 cut(s)	1265, 3324
GsaI	CCCAGC	2 cut(s)	387, 1500
GsuI	CTGGAG	3 cut(s)	2262, 2693, 2891
HaeII	RGCGCY	1 cut(s)	3326
HaeIII	GGCC	3 cut(s)	1748, 2231, 3060
HapII	CCGG	2 cut(s)	269, 3192
HgaI	GACGC	3 cut(s)	1857, 3265, 3375
HhaI	GCGC	2 cut(s)	1266, 3325
Hin1I	GRCGYC	2 cut(s)	514, 3367
Hin6I	GCGC	2 cut(s)	1264, 3323
HinP1I	GCGC	2 cut(s)	1264, 3323
HincII	GTYRAC	1 cut(s)	2854
HindII	GTYRAC	1 cut(s)	2854
HindIII	AAGCTT	3 cut(s)	846, 1103, 2336
HpaII	CCGG	2 cut(s)	269, 3192
HphI	GGTGA	6 cut(s)	1379, 1621, 2489, 2948, 3059, 3091
Hpy166II	GTNNAC	2 cut(s)	1689, 2854
Hpy8I	GTNNAC	2 cut(s)	1689, 2854
Hpy99I	CGWCG	2 cut(s)	763, 3369
HpyCH4III	ACNGT	3 cut(s)	1723, 2841, 3244
HpyCH4IV	ACGT	5 cut(s)	514, 1123, 1213, 2774, 2896
HpyF10VI	GCNNNNNNNGC	8 cut(s)	10, 652, 1261, 1700, 2081, 2228, 2381, 2677
HpySE526I	ACGT	5 cut(s)	514, 1123, 1213, 2774, 2896
Hsp92I	GRCGYC	2 cut(s)	514, 3367
HspAI	GCGC	2 cut(s)	1264, 3323
Ksp22I	TGATCA	4 cut(s)	1351, 1840, 2209, 2266
LguI	GCTCTTC	1 cut(s)	1337
LmnI	GCTCC	6 cut(s)	401, 691, 1621, 2674, 3295, 3511
MaeI	CTAG	6 cut(s)	1716, 1775, 2223, 2435, 2514, 3018
MaeII	ACGT	5 cut(s)	514, 1123, 1213, 2774, 2896
MbiI	CCGCTC	1 cut(s)	1147
MfeI	CAATTG	3 cut(s)	82, 303, 2585
MflI	RGATCY	8 cut(s)	826, 931, 1401, 1735, 2290, 2323, 3149, 3328
MhlI	GDGCHC	4 cut(s)	213, 1618, 2086, 2255
MlsI	TGGCCA	3 cut(s)	1748, 2231, 3060
MluNI	TGGCCA	3 cut(s)	1748, 2231, 3060
MlyI	GAGTC	3 cut(s)	1003, 2269, 3199
MmeI	TCCRAC	4 cut(s)	1903, 3331, 3388, 3436
Mox20I	TGGCCA	3 cut(s)	1748, 2231, 3060
MroXI	GAANNNNTTC	1 cut(s)	2554
MscI	TGGCCA	3 cut(s)	1748, 2231, 3060
MslI	CAYNNNNRTG	2 cut(s)	201, 497
Msp20I	TGGCCA	3 cut(s)	1748, 2231, 3060
MspA1I	CMGCKG	1 cut(s)	2671
MspCI	CTTAAG	3 cut(s)	476, 2111, 2414
MspI	CCGG	2 cut(s)	269, 3192
MspR9I	CCNGG	5 cut(s)	1365, 1425, 3135, 3193, 3371
MunI	CAATTG	3 cut(s)	82, 303, 2585
Mva1269I	GAATGC	2 cut(s)	2134, 3521
MvaI	CCWGG	4 cut(s)	1365, 1425, 3135, 3371
MwoI	GCNNNNNNNGC	8 cut(s)	10, 652, 1261, 1700, 2081, 2228, 2381, 2677
NciI	CCSGG	1 cut(s)	3193
NcoI	CCATGG	1 cut(s)	3061
NlaIV	GGNNCC	6 cut(s)	212, 1559, 1617, 1653, 2254, 3330
NmuCI	GTSAC	4 cut(s)	149, 173, 1209, 3065
NspI	RCATGY	3 cut(s)	188, 2243, 2349
NspV	TTCGAA	1 cut(s)	169
OliI	CACNNNNGTG	1 cut(s)	497
PaeI	GCATGC	2 cut(s)	188, 2243
PaeR7I	CTCGAG	1 cut(s)	495
PagI	TCATGA	2 cut(s)	1065, 1866
PaqCI	CACCTGC	1 cut(s)	2924
PciSI	GCTCTTC	1 cut(s)	1337
PctI	GAATGC	2 cut(s)	2134, 3521
PdmI	GAANNNNTTC	1 cut(s)	2554
PfeI	GAWTC	9 cut(s)	455, 1009, 1016, 1057, 1163, 1193, 1870, 2494, 2987
PflFI	GACNNNGTC	1 cut(s)	3208
PflMI	CCANNNNNTGG	1 cut(s)	715
PinAI	ACCGGT	1 cut(s)	268
Ple19I	CGATCG	1 cut(s)	181
PleI	GAGTC	3 cut(s)	1002, 2269, 3199
PmaCI	CACGTG	1 cut(s)	1214
PmlI	CACGTG	1 cut(s)	1214
PpsI	GAGTC	3 cut(s)	1002, 2269, 3199
Ppu21I	YACGTR	1 cut(s)	1214
PsiI	TTATAA	2 cut(s)	20, 3398
Psp6I	CCWGG	4 cut(s)	1363, 1423, 3133, 3369
PspCI	CACGTG	1 cut(s)	1214
PspEI	GGTNACC	2 cut(s)	3065, 3095
PspFI	CCCAGC	2 cut(s)	383, 1496
PspGI	CCWGG	4 cut(s)	1363, 1423, 3133, 3369
PspN4I	GGNNCC	6 cut(s)	212, 1559, 1617, 1653, 2254, 3330
PspPI	GGNCC	2 cut(s)	1436, 3131
PspXI	VCTCGAGB	1 cut(s)	495
PstI	CTGCAG	2 cut(s)	1002, 1409
PstNI	CAGNNNCTG	3 cut(s)	997, 2503, 2683
PsuI	RGATCY	8 cut(s)	826, 931, 1401, 1735, 2290, 2323, 3149, 3328
PsyI	GACNNNGTC	1 cut(s)	3208
PvuI	CGATCG	1 cut(s)	181
PvuII	CAGCTG	1 cut(s)	2671
RsaI	GTAC	6 cut(s)	144, 584, 1153, 1688, 2723, 3338
RsaNI	GTAC	6 cut(s)	143, 583, 1152, 1687, 2722, 3337
RseI	CAYNNNNRTG	2 cut(s)	201, 497
SapI	GCTCTTC	1 cut(s)	1337
Sau96I	GGNCC	2 cut(s)	1436, 3131
ScaI	AGTACT	2 cut(s)	584, 2723
SchI	GAGTC	3 cut(s)	1003, 2269, 3199
ScrFI	CCNGG	5 cut(s)	1365, 1425, 3135, 3193, 3371
SduI	GDGCHC	4 cut(s)	213, 1618, 2086, 2255
SfcI	CTRYAG	5 cut(s)	40, 935, 989, 998, 1405
Sfr274I	CTCGAG	1 cut(s)	495
SfuI	TTCGAA	1 cut(s)	169
SinI	GGWCC	2 cut(s)	1436, 3131
SlaI	CTCGAG	1 cut(s)	495
SmiMI	CAYNNNNRTG	2 cut(s)	201, 497
SmlI	CTYRAG	8 cut(s)	476, 495, 914, 1031, 1919, 2111, 2299, 2414
SmoI	CTYRAG	8 cut(s)	476, 495, 914, 1031, 1919, 2111, 2299, 2414
SphI	GCATGC	2 cut(s)	188, 2243
SsiI	CCGC	5 cut(s)	289, 1147, 2197, 2354, 2422
SspI	AATATT	1 cut(s)	1420
SspMI	CTAG	6 cut(s)	1716, 1775, 2223, 2435, 2514, 3018
StyD4I	CCNGG	5 cut(s)	1363, 1423, 3133, 3191, 3369
StyI	CCWWGG	4 cut(s)	801, 1619, 2598, 3061
TaaI	ACNGT	3 cut(s)	1723, 2841, 3244
TaiI	ACGT	5 cut(s)	517, 1126, 1216, 2777, 2899
TatI	WGTACW	5 cut(s)	142, 582, 1686, 2721, 3336
TfiI	GAWTC	9 cut(s)	455, 1009, 1016, 1057, 1163, 1193, 1870, 2494, 2987
TscAI	CASTG	6 cut(s)	450, 550, 876, 1488, 1575, 1696
TseFI	GTSAC	4 cut(s)	149, 173, 1209, 3065
Tsp45I	GTSAC	4 cut(s)	149, 173, 1209, 3065
TspRI	CASTG	6 cut(s)	450, 550, 876, 1488, 1575, 1696
Tth111I	GACNNNGTC	1 cut(s)	3208
Van91I	CCANNNNNTGG	1 cut(s)	715
Vha464I	CTTAAG	3 cut(s)	476, 2111, 2414
VpaK11BI	GGWCC	2 cut(s)	1436, 3131
XapI	RAATTY	3 cut(s)	367, 1387, 2881
XbaI	TCTAGA	1 cut(s)	1774
XceI	RCATGY	3 cut(s)	188, 2243, 2349
XhoI	CTCGAG	1 cut(s)	495
XmnI	GAANNNNTTC	1 cut(s)	2554
XspI	CTAG	6 cut(s)	1716, 1775, 2223, 2435, 2514, 3018
ZraI	GACGTC	1 cut(s)	515
ZrmI	AGTACT	2 cut(s)	584, 2723

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.