MD03G1289100.v1.1

Belongs to the eukaryotic ribosomal protein eL13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001375
Physical Location & Seq
Forward (+)
36746999 .. 36749078
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1289100.v1.1.491

Sequence Viewer

Length: 624 bp
ATGGTGAAGCACAACAACGTTATTCCGAGCTCTCACTTCAGGAAGCATTGGCAGAACTATGTCAAGACCTGGTTCAACCAACCTGCTAGGAAGACCCGCAGAAGAACTGCCCGACAGAAGAAGGCCGTGAAGATCTTCCCCCGTCCAACCACTGGTCCACTCCGTCCCATTGTTCATGGACAGACATTGAAGTATAACATGAAAGTGAGGGCTGGCAGGGGCTTTACTCTGGAGGAGTTGAAGTCTGCTGGGATTCCAAAGAAACTTGCACCAACCATTGGAATTTCAGTTGATCATCGCAGGAAGAACCGTTCTCTTGAGGGACTTCAAGCTAATGTGCAGCGCCTGAAGACATACAAGGCCAAATTAGTGGTCTTCCCAAGACGCGCTAAGCAGTTCAAGGCCGGCGATTCTCCTGCAGAGGAGCTTGCCAATGCCACCCAGCTCCACGCCCCCTACCTTCCAATTGCACGCGAGAAGCCTACAGTTGAGCTTGTGAAGGTTACCGATGAGTTGAAGTCGTTCAAGGCATATGACAAACTCCGTGTGGAGAGGATGAACAAACGCCATGTTGGTGCCAGGCTTAAGAAGGCAGCTGAGGCCGAAAAGGAAGAAAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L13e

Protein Analysis

208

Amino Acids

23.89

Weight (kDa)

10.92

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L13e PF01294 7 - 185 1.2e-78 Ribosomal protein L13e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 91
AccB1I GGYRCC 1 cut(s) 575
AccB7I CCANNNNNTGG 2 cut(s) 152, 278
AccII CGCG 2 cut(s) 387, 474
AciI CCGC 1 cut(s) 97
AclI AACGTT 1 cut(s) 18
AcsI RAATTY 1 cut(s) 282
AcuI CTGAAG 2 cut(s) 22, 368
AfiI CCNNNNNNNGG 2 cut(s) 152, 278
AflII CTTAAG 1 cut(s) 584
AgsI TTSAA 7 cut(s) 76, 190, 241, 329, 400, 517, 526
AjnI CCWGG 2 cut(s) 68, 578
AloI GAACNNNNNNTCC 2 cut(s) 295, 327
AluBI AGCT 6 cut(s) 30, 332, 427, 445, 493, 596
AluI AGCT 6 cut(s) 30, 332, 427, 445, 493, 596
Alw21I GWGCWC 1 cut(s) 32
AlwNI CAGNNNCTG 1 cut(s) 346
AoxI GGCC 4 cut(s) 123, 360, 402, 600
ApeKI GCWGC 2 cut(s) 340, 593
ApoI RAATTY 1 cut(s) 282
ArsI GACNNNNNNTTYG 2 cut(s) 357, 389
Asp700I GAANNNNTTC 2 cut(s) 134, 521
AspLEI GCGC 2 cut(s) 345, 389
AspS9I GGNCC 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 16
AvaII GGWCC 1 cut(s) 155
BanI GGYRCC 1 cut(s) 575
BanII GRGCYC 1 cut(s) 32
BbsI GAAGAC 3 cut(s) 98, 356, 367
Bbv12I GWGCWC 1 cut(s) 32
BbvCI CCTCAGC 1 cut(s) 597
BbvI GCAGC 2 cut(s) 352, 605
BceAI ACGGC 1 cut(s) 110
BcgI CGANNNNNNTGC 2 cut(s) 398, 432
BciT130I CCWGG 2 cut(s) 70, 580
BclI TGATCA 1 cut(s) 292
BfaI CTAG 1 cut(s) 87
BfmI CTRYAG 2 cut(s) 417, 483
BfoI RGCGCY 1 cut(s) 346
BfrI CTTAAG 1 cut(s) 584
BfuAI ACCTGC 1 cut(s) 91
BglII AGATCT 1 cut(s) 132
BisI GCNGC 2 cut(s) 341, 594
BlpI GCTNAGC 1 cut(s) 390
BlsI GCNGC 2 cut(s) 342, 595
Bme1390I CCNGG 2 cut(s) 70, 580
Bme18I GGWCC 1 cut(s) 155
BmgT120I GGNCC 1 cut(s) 155
BmiI GGNNCC 1 cut(s) 577
BmrFI CCNGG 2 cut(s) 70, 580
BpiI GAAGAC 3 cut(s) 98, 356, 367
BpmI CTGGAG 1 cut(s) 251
Bpu10I CCTNAGC 1 cut(s) 597
Bpu1102I GCTNAGC 1 cut(s) 390
BpuEI CTTGAG 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 2 cut(s) 152, 278
Bse118I RCCGGY 1 cut(s) 404
Bse1I ACTGG 1 cut(s) 157
BseBI CCWGG 2 cut(s) 70, 580
BseGI GGATG 1 cut(s) 561
BseLI CCNNNNNNNGG 2 cut(s) 152, 278
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 2 cut(s) 248, 437
BseXI GCAGC 2 cut(s) 352, 605
BseYI CCCAGC 2 cut(s) 248, 441
BsgI GTGCAG 1 cut(s) 359
Bsh1236I CGCG 2 cut(s) 387, 474
BshFI GGCC 4 cut(s) 125, 362, 404, 602
BshNI GGYRCC 1 cut(s) 575
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 1 cut(s) 405
BslFI GGGAC 2 cut(s) 150, 336
BslI CCNNNNNNNGG 2 cut(s) 152, 278
BsmFI GGGAC 2 cut(s) 150, 336
BsnI GGCC 4 cut(s) 125, 362, 404, 602
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 2 cut(s) 132, 292
Bsp1720I GCTNAGC 1 cut(s) 390
BspACI CCGC 1 cut(s) 97
BspANI GGCC 4 cut(s) 125, 362, 404, 602
BspCNI CTCAG 1 cut(s) 589
BspFNI CGCG 2 cut(s) 387, 474
BspLI GGNNCC 1 cut(s) 577
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 91
BspT107I GGYRCC 1 cut(s) 575
BspTI CTTAAG 1 cut(s) 584
BsrFI RCCGGY 1 cut(s) 404
BsrI ACTGG 1 cut(s) 157
BssAI RCCGGY 1 cut(s) 404
BssMI GATC 2 cut(s) 132, 292
Bst2UI CCWGG 2 cut(s) 70, 580
Bst4CI ACNGT 2 cut(s) 311, 487
BstAFI CTTAAG 1 cut(s) 584
BstC8I GCNNGC 4 cut(s) 214, 406, 429, 472
BstDEI CTNAG 2 cut(s) 390, 597
BstEII GGTNACC 1 cut(s) 502
BstF5I GGATG 1 cut(s) 561
BstFNI CGCG 2 cut(s) 387, 474
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 2 cut(s) 345, 389
BstKTI GATC 2 cut(s) 135, 295
BstMBI GATC 2 cut(s) 132, 292
BstMWI GCNNNNNNNGC 1 cut(s) 599
BstNI CCWGG 2 cut(s) 70, 580
BstPI GGTNACC 1 cut(s) 502
BstSCI CCNGG 2 cut(s) 68, 578
BstSFI CTRYAG 2 cut(s) 417, 483
BstUI CGCG 2 cut(s) 387, 474
BstV1I GCAGC 2 cut(s) 352, 605
BstV2I GAAGAC 3 cut(s) 98, 356, 367
BstX2I RGATCY 1 cut(s) 132
BstXI CCANNNNNNTGG 1 cut(s) 370
BstYI RGATCY 1 cut(s) 132
BsuRI GGCC 4 cut(s) 125, 362, 404, 602
BtgZI GCGATG 1 cut(s) 281
BtsCI GGATG 1 cut(s) 561
BtsIMutI CAGTG 1 cut(s) 150
BveI ACCTGC 1 cut(s) 91
Cac8I GCNNGC 4 cut(s) 214, 406, 429, 472
CaiI CAGNNNCTG 1 cut(s) 346
CfoI GCGC 2 cut(s) 345, 389
Cfr10I RCCGGY 1 cut(s) 404
Cfr13I GGNCC 1 cut(s) 155
CseI GACGC 1 cut(s) 393
CsiI ACCWGGT 1 cut(s) 68
CviAII CATG 3 cut(s) 176, 199, 569
DdeI CTNAG 2 cut(s) 390, 597
DpnI GATC 2 cut(s) 134, 294
DpnII GATC 2 cut(s) 132, 292
Ecl136II GAGCTC 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 32
Eco47I GGWCC 1 cut(s) 155
Eco53kI GAGCTC 1 cut(s) 30
Eco57I CTGAAG 2 cut(s) 22, 368
Eco91I GGTNACC 1 cut(s) 502
EcoICRI GAGCTC 1 cut(s) 30
EcoO65I GGTNACC 1 cut(s) 502
EcoRII CCWGG 2 cut(s) 68, 578
EcoT38I GRGCYC 1 cut(s) 32
FaeI CATG 3 cut(s) 179, 202, 572
FaiI YATR 8 cut(s) 60, 177, 195, 200, 355, 532, 534, 570
FaqI GGGAC 2 cut(s) 150, 336
FatI CATG 3 cut(s) 175, 198, 568
FauI CCCGC 1 cut(s) 104
FauNDI CATATG 1 cut(s) 532
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 2 cut(s) 341, 594
FokI GGATG 1 cut(s) 568
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 2 cut(s) 341, 594
FspBI CTAG 1 cut(s) 87
GlaI GCGC 2 cut(s) 344, 388
GluI GCNGC 2 cut(s) 341, 594
GsaI CCCAGC 2 cut(s) 252, 445
GsuI CTGGAG 1 cut(s) 251
HaeII RGCGCY 1 cut(s) 346
HaeIII GGCC 4 cut(s) 125, 362, 404, 602
HapII CCGG 1 cut(s) 405
HgaI GACGC 1 cut(s) 393
HhaI GCGC 2 cut(s) 345, 389
Hin1II CATG 3 cut(s) 179, 202, 572
Hin6I GCGC 2 cut(s) 343, 387
HinP1I GCGC 2 cut(s) 343, 387
HinfI GANTC 2 cut(s) 253, 410
HpaII CCGG 1 cut(s) 405
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 158
Hpy188I TCNGA 1 cut(s) 27
Hpy188III TCNNGA 4 cut(s) 40, 64, 230, 317
Hpy8I GTNNAC 1 cut(s) 158
HpyAV CCTTC 4 cut(s) 115, 470, 493, 583
HpyCH4III ACNGT 2 cut(s) 311, 487
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 4 cut(s) 269, 340, 419, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 599
HpyF3I CTNAG 2 cut(s) 390, 597
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 3 cut(s) 179, 202, 572
HspAI GCGC 2 cut(s) 343, 387
KroI GCCGGC 1 cut(s) 404
KroNI GCCGGC 1 cut(s) 406
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 2 cut(s) 132, 292
LmnI GCTCC 2 cut(s) 424, 450
Lsp1109I GCAGC 2 cut(s) 352, 605
MabI ACCWGGT 1 cut(s) 68
MaeI CTAG 1 cut(s) 87
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 502
MalI GATC 2 cut(s) 134, 294
MboI GATC 2 cut(s) 132, 292
MboII GAAGA 9 cut(s) 103, 114, 127, 130, 142, 316, 361, 367, 623
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 3 cut(s) 282, 365, 465
MmeI TCCRAC 1 cut(s) 170
MnlI CCTC 6 cut(s) 201, 226, 313, 415, 546, 592
MroNI GCCGGC 1 cut(s) 404
MroXI GAANNNNTTC 2 cut(s) 134, 521
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 2 cut(s) 203, 573
MspA1I CMGCKG 1 cut(s) 596
MspCI CTTAAG 1 cut(s) 584
MspI CCGG 1 cut(s) 405
MspR9I CCNGG 2 cut(s) 70, 580
MunI CAATTG 1 cut(s) 465
MvaI CCWGG 2 cut(s) 70, 580
MvnI CGCG 2 cut(s) 387, 474
MwoI GCNNNNNNNGC 1 cut(s) 599
NaeI GCCGGC 1 cut(s) 406
NdeI CATATG 1 cut(s) 532
NdeII GATC 2 cut(s) 132, 292
NgoMIV GCCGGC 1 cut(s) 404
NlaIII CATG 3 cut(s) 179, 202, 572
NlaIV GGNNCC 1 cut(s) 577
PdiI GCCGGC 1 cut(s) 406
PdmI GAANNNNTTC 2 cut(s) 134, 521
PfeI GAWTC 2 cut(s) 253, 410
PflMI CCANNNNNTGG 2 cut(s) 152, 278
PkrI GCNGC 2 cut(s) 342, 595
Psp124BI GAGCTC 1 cut(s) 32
Psp1406I AACGTT 1 cut(s) 18
Psp6I CCWGG 2 cut(s) 68, 578
PspEI GGTNACC 1 cut(s) 502
PspFI CCCAGC 2 cut(s) 248, 441
PspGI CCWGG 2 cut(s) 68, 578
PspN4I GGNNCC 1 cut(s) 577
PspPI GGNCC 1 cut(s) 155
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 346
PsuI RGATCY 1 cut(s) 132
PvuII CAGCTG 1 cut(s) 596
RseI CAYNNNNRTG 2 cut(s) 203, 573
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 2 cut(s) 341, 594
Sau3AI GATC 2 cut(s) 132, 292
Sau96I GGNCC 1 cut(s) 155
ScrFI CCNGG 2 cut(s) 70, 580
SduI GDGCHC 1 cut(s) 32
SexAI ACCWGGT 1 cut(s) 68
SfcI CTRYAG 2 cut(s) 417, 483
SinI GGWCC 1 cut(s) 155
SmiMI CAYNNNNRTG 2 cut(s) 203, 573
SmlI CTYRAG 2 cut(s) 317, 584
SmoI CTYRAG 2 cut(s) 317, 584
Sse9I AATT 3 cut(s) 282, 365, 465
SsiI CCGC 1 cut(s) 97
SspMI CTAG 1 cut(s) 87
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 2 cut(s) 68, 578
TaaI ACNGT 2 cut(s) 311, 487
TaiI ACGT 1 cut(s) 21
TasI AATT 3 cut(s) 282, 365, 465
TfiI GAWTC 2 cut(s) 253, 410
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 1 cut(s) 157
TseI GCWGC 2 cut(s) 340, 593
TspDTI ATGAA 3 cut(s) 164, 215, 572
TspGWI ACGGA 2 cut(s) 152, 533
TspRI CASTG 1 cut(s) 157
Van91I CCANNNNNTGG 2 cut(s) 152, 278
Vha464I CTTAAG 1 cut(s) 584
VpaK11BI GGWCC 1 cut(s) 155
XapI RAATTY 1 cut(s) 282
XmnI GAANNNNTTC 2 cut(s) 134, 521
XspI CTAG 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.