MD04G1155500.v1.1

SelT-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003155
Physical Location & Seq
Forward (+)
24320089 .. 24320797
709 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1155500.v1.1.491

Sequence Viewer

Length: 372 bp
ATGACGCCGATGATCCTCACCATCCAGACCCTCAGCAAGAACAGCTTCTCCAAGATCCCCTCAAATTCCCTACCCAGAAGTGGCAGTGGGGGATTTGGCATTGGATGCACCATCAATATTGATTTTTGTACATCTTGTTCATTTCCCCGGATCGATGTAACCCTTCCCTTCCAACTTCCAAGGCGCCTGCTGAGCAAACTGGTACCGGTTGTTCAAGTAGGAATCATCGGGACGATAATGGTCGGCAAACATGTGTTCTCGGGGCTGGGAATAACGACTCCACCTCCTTGGTACCAGGCCTTGCTAGCAAGTAGGTTTGGAAGTATGGCAAGCACTTGGCTTTTTGGAAACTTCATCCAATCCTTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rdx

Protein Analysis

124

Amino Acids

13.33

Weight (kDa)

10.21

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 202, 291
AccB1I GGYRCC 3 cut(s) 183, 202, 291
AclWI GGATC 3 cut(s) 7, 49, 158
AcsI RAATTY 1 cut(s) 64
AcyI GRCGYC 2 cut(s) 5, 184
AfaI GTAC 3 cut(s) 130, 204, 293
AfiI CCNNNNNNNGG 1 cut(s) 80
AflIII ACRYGT 1 cut(s) 250
AgeI ACCGGT 1 cut(s) 205
AgsI TTSAA 1 cut(s) 215
AjnI CCWGG 1 cut(s) 294
AloI GAACNNNNNNTCC 2 cut(s) 32, 64
AluBI AGCT 1 cut(s) 45
AluI AGCT 1 cut(s) 45
AlwI GGATC 3 cut(s) 7, 49, 158
Ama87I CYCGRG 1 cut(s) 259
AoxI GGCC 1 cut(s) 297
ApoI RAATTY 1 cut(s) 64
AsiGI ACCGGT 1 cut(s) 205
Asp700I GAANNNNTTC 1 cut(s) 44
Asp718I GGTACC 2 cut(s) 202, 291
AspLEI GCGC 1 cut(s) 186
AsuC2I CCSGG 1 cut(s) 148
AsuHPI GGTGA 1 cut(s) 10
AsuNHI GCTAGC 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 259
BanI GGYRCC 3 cut(s) 183, 202, 291
BbvCI CCTCAGC 1 cut(s) 32
BccI CCATC 2 cut(s) 29, 119
BciT130I CCWGG 1 cut(s) 296
BcnI CCSGG 1 cut(s) 148
BfaI CTAG 1 cut(s) 305
BfoI RGCGCY 1 cut(s) 187
BlpI GCTNAGC 1 cut(s) 191
Bme1390I CCNGG 2 cut(s) 148, 296
BmeT110I CYCGRG 1 cut(s) 259
BmiI GGNNCC 3 cut(s) 185, 204, 293
BmrFI CCNGG 2 cut(s) 148, 296
BmsI GCATC 1 cut(s) 95
BmtI GCTAGC 1 cut(s) 308
Bpu10I CCTNAGC 1 cut(s) 32
Bpu1102I GCTNAGC 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 148
Bsa29I ATCGAT 1 cut(s) 153
BsaHI GRCGYC 2 cut(s) 5, 184
BsaJI CCNNGG 3 cut(s) 146, 179, 287
BsaWI WCCGGW 1 cut(s) 205
BsaXI ACNNNNNCTCC 4 cut(s) 32, 62, 268, 298
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 1 cut(s) 204
BseBI CCWGG 1 cut(s) 296
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 3 cut(s) 146, 179, 287
BseGI GGATG 3 cut(s) 21, 110, 354
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMII CTCAG 2 cut(s) 46, 182
BseNI ACTGG 1 cut(s) 204
BseYI CCCAGC 1 cut(s) 265
BshFI GGCC 1 cut(s) 299
BshNI GGYRCC 3 cut(s) 183, 202, 291
BshTI ACCGGT 1 cut(s) 205
BshVI ATCGAT 1 cut(s) 153
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 2 cut(s) 148, 206
BslFI GGGAC 1 cut(s) 244
BslI CCNNNNNNNGG 1 cut(s) 80
BsmFI GGGAC 1 cut(s) 244
BsnI GGCC 1 cut(s) 299
BsoBI CYCGRG 1 cut(s) 259
Bsp1407I TGTACA 1 cut(s) 128
Bsp143I GATC 3 cut(s) 12, 54, 150
Bsp1720I GCTNAGC 1 cut(s) 191
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 2 cut(s) 45, 183
BspDI ATCGAT 1 cut(s) 153
BspLI GGNNCC 3 cut(s) 185, 204, 293
BspOI GCTAGC 1 cut(s) 308
BspPI GGATC 3 cut(s) 7, 49, 158
BspT107I GGYRCC 3 cut(s) 183, 202, 291
BsrFI RCCGGY 1 cut(s) 205
BsrGI TGTACA 1 cut(s) 128
BsrI ACTGG 1 cut(s) 204
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 3 cut(s) 146, 179, 287
BssMI GATC 3 cut(s) 12, 54, 150
BssNI GRCGYC 2 cut(s) 5, 184
BssT1I CCWWGG 2 cut(s) 179, 287
Bst2UI CCWGG 1 cut(s) 296
BstACI GRCGYC 2 cut(s) 5, 184
BstAPI GCANNNNNTGC 1 cut(s) 105
BstAUI TGTACA 1 cut(s) 128
BstC8I GCNNGC 3 cut(s) 188, 306, 331
BstDEI CTNAG 2 cut(s) 32, 191
BstF5I GGATG 3 cut(s) 21, 110, 354
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 3 cut(s) 15, 57, 153
BstMBI GATC 3 cut(s) 12, 54, 150
BstMWI GCNNNNNNNGC 4 cut(s) 42, 105, 192, 305
BstNI CCWGG 1 cut(s) 296
BstNSI RCATGY 1 cut(s) 254
BstSCI CCNGG 2 cut(s) 146, 294
BstX2I RGATCY 1 cut(s) 54
BstXI CCANNNNNNTGG 1 cut(s) 288
BstYI RGATCY 1 cut(s) 54
Bsu15I ATCGAT 1 cut(s) 153
BsuRI GGCC 1 cut(s) 299
BsuTUI ATCGAT 1 cut(s) 153
BtsCI GGATG 3 cut(s) 21, 110, 354
BtsI GCAGTG 1 cut(s) 91
BtsIMutI CAGTG 1 cut(s) 91
Cac8I GCNNGC 3 cut(s) 188, 306, 331
CfoI GCGC 1 cut(s) 186
Cfr10I RCCGGY 1 cut(s) 205
ClaI ATCGAT 1 cut(s) 153
CseI GACGC 1 cut(s) 13
Csp6I GTAC 3 cut(s) 129, 203, 292
CspAI ACCGGT 1 cut(s) 205
CviAII CATG 1 cut(s) 251
CviJI RGCY 4 cut(s) 45, 265, 299, 340
CviKI_1 RGCY 4 cut(s) 45, 265, 299, 340
CviQI GTAC 3 cut(s) 129, 203, 292
DdeI CTNAG 2 cut(s) 32, 191
DinI GGCGCC 1 cut(s) 185
DpnI GATC 3 cut(s) 14, 56, 152
DpnII GATC 3 cut(s) 12, 54, 150
Eco130I CCWWGG 2 cut(s) 179, 287
Eco147I AGGCCT 1 cut(s) 299
Eco88I CYCGRG 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 294
EcoT14I CCWWGG 2 cut(s) 179, 287
EgeI GGCGCC 1 cut(s) 185
EheI GGCGCC 1 cut(s) 185
ErhI CCWWGG 2 cut(s) 179, 287
FaeI CATG 1 cut(s) 254
FaiI YATR 2 cut(s) 252, 326
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FaqI GGGAC 1 cut(s) 244
FatI CATG 1 cut(s) 250
FokI GGATG 3 cut(s) 8, 117, 341
FspBI CTAG 1 cut(s) 305
GlaI GCGC 1 cut(s) 185
GsaI CCCAGC 1 cut(s) 269
HaeII RGCGCY 1 cut(s) 187
HaeIII GGCC 1 cut(s) 299
HapII CCGG 2 cut(s) 148, 206
HgaI GACGC 1 cut(s) 13
HhaI GCGC 1 cut(s) 186
Hin1I GRCGYC 2 cut(s) 5, 184
Hin1II CATG 1 cut(s) 254
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HinfI GANTC 2 cut(s) 222, 277
HpaII CCGG 2 cut(s) 148, 206
HphI GGTGA 1 cut(s) 10
Hpy188III TCNNGA 2 cut(s) 25, 229
HpyAV CCTTC 2 cut(s) 173, 178
HpyCH4V TGCA 1 cut(s) 108
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 105, 192, 305
HpyF3I CTNAG 2 cut(s) 32, 191
Hsp92I GRCGYC 2 cut(s) 5, 184
Hsp92II CATG 1 cut(s) 254
HspAI GCGC 1 cut(s) 184
KasI GGCGCC 1 cut(s) 183
KpnI GGTACC 2 cut(s) 206, 295
Kzo9I GATC 3 cut(s) 12, 54, 150
LpnPI CCDG 9 cut(s) 38, 88, 161, 185, 200, 219, 251, 281, 308
LweI GCATC 1 cut(s) 95
MaeI CTAG 1 cut(s) 305
MaeIII GTNAC 1 cut(s) 157
MalI GATC 3 cut(s) 14, 56, 152
MboI GATC 3 cut(s) 12, 54, 150
MflI RGATCY 1 cut(s) 54
MluCI AATT 1 cut(s) 64
Mly113I GGCGCC 1 cut(s) 184
MlyI GAGTC 1 cut(s) 271
MmeI TCCRAC 1 cut(s) 196
MnlI CCTC 4 cut(s) 26, 41, 70, 294
MroXI GAANNNNTTC 1 cut(s) 44
MseI TTAA 1 cut(s) 370
MspI CCGG 2 cut(s) 148, 206
MspR9I CCNGG 2 cut(s) 148, 296
MvaI CCWGG 1 cut(s) 296
MwoI GCNNNNNNNGC 4 cut(s) 42, 105, 192, 305
NarI GGCGCC 1 cut(s) 184
NciI CCSGG 1 cut(s) 148
NdeII GATC 3 cut(s) 12, 54, 150
NheI GCTAGC 1 cut(s) 304
NlaIII CATG 1 cut(s) 254
NlaIV GGNNCC 3 cut(s) 185, 204, 293
NspI RCATGY 1 cut(s) 254
PceI AGGCCT 1 cut(s) 299
PciI ACATGT 1 cut(s) 250
PdmI GAANNNNTTC 1 cut(s) 44
PfeI GAWTC 1 cut(s) 222
PinAI ACCGGT 1 cut(s) 205
PleI GAGTC 1 cut(s) 271
PluTI GGCGCC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 271
PscI ACATGT 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 294
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 3 cut(s) 185, 204, 293
PsrI GAACNNNNNNTAC 4 cut(s) 121, 153, 195, 227
PsuI RGATCY 1 cut(s) 54
RsaI GTAC 3 cut(s) 130, 204, 293
RsaNI GTAC 3 cut(s) 129, 203, 292
SaqAI TTAA 1 cut(s) 370
Sau3AI GATC 3 cut(s) 12, 54, 150
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 2 cut(s) 148, 296
SetI ASST 3 cut(s) 47, 286, 317
SfaNI GCATC 1 cut(s) 95
SfoI GGCGCC 1 cut(s) 185
Sse9I AATT 1 cut(s) 64
SseBI AGGCCT 1 cut(s) 299
SspDI GGCGCC 1 cut(s) 183
SspI AATATT 1 cut(s) 118
SspMI CTAG 1 cut(s) 305
StuI AGGCCT 1 cut(s) 299
StyD4I CCNGG 2 cut(s) 146, 294
StyI CCWWGG 2 cut(s) 179, 287
TaqI TCGA 1 cut(s) 153
TasI AATT 1 cut(s) 64
TatI WGTACW 1 cut(s) 128
TfiI GAWTC 1 cut(s) 222
Tru1I TTAA 1 cut(s) 370
Tru9I TTAA 1 cut(s) 370
TscAI CASTG 1 cut(s) 91
TspDTI ATGAA 2 cut(s) 129, 343
TspRI CASTG 1 cut(s) 91
XapI RAATTY 1 cut(s) 64
XceI RCATGY 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 44
XspI CTAG 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.