MD12G1168700.v1.1

SelT-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003155
Physical Location & Seq
Forward (+)
24905752 .. 24907239
1488 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1168700.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGATCGAGCTCTGTTAGTTCTGGTAGGGTTATCGATCTTCCTGTTCTGCACTGATATTTTTAACATTTTCACGCCCAAACCTCCCAAAACTACCACTCATTATCCTCTTCTTGATGCCGATGATCCTCACCAAGCAGACCCCCAGCTAGAACAGCTTCTCCAAGAGCCCCTCAAATTCCCCACCCACCAGAAATCTAATGGCTTTGGGGGAGTTGGCATTGGAAGCACCATCAATATTGATTTCTGTACATCTTGTTCTTACAGAGGAACTGCAATAACATTGAAAAACATGCTGGTAACATCATTTCCCGGGATCGATGTAATACTGGCAAACCACCACCCTCCCCTTCCAAGACGCCTGCTGAGCAAACTGGTACCGGTTGTTCAAGTAGGAATCATCGGGACTATAATGGCCGGAAAACATGTTTTCTCAAGGCTGGGGATAACGACGCCACCTCCTTGGTACCAGACCCTGCAAGCAAATAGGTTTGGCAGTATGGCAAGCACTTGGCTTTTGGGAAACTTCATCCAATCCTTCCTTCAAAGCACAGGAGCGTTTGAAGTGTATTGTAATGGTGAATTGGTCTTCTCGAAGCTGAAGGAGCAGAGGTTCCCCAGTGAAACTGAGTTGAGGGATCTCGTCGGAAAGAAGCTTGGAGGAGGGGTCTGGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rdx

Protein Analysis

226

Amino Acids

24.83

Weight (kDa)

8.69

Isoelectric Point (pI)

37.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rdx PF10262 77 - 215 3.7e-19 Rdx family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 376, 465
AccB1I GGYRCC 2 cut(s) 376, 465
AclWI GGATC 4 cut(s) 12, 119, 323, 645
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 1 cut(s) 176
AcuI CTGAAG 1 cut(s) 620
AcyI GRCGYC 2 cut(s) 358, 452
AfaI GTAC 3 cut(s) 250, 378, 467
AflIII ACRYGT 1 cut(s) 424
AgeI ACCGGT 1 cut(s) 379
AgsI TTSAA 4 cut(s) 286, 389, 545, 563
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
AloI GAACNNNNNNTCC 4 cut(s) 144, 176, 596, 628
AluBI AGCT 5 cut(s) 11, 148, 157, 598, 655
AluI AGCT 5 cut(s) 11, 148, 157, 598, 655
Alw21I GWGCWC 1 cut(s) 13
AlwI GGATC 4 cut(s) 12, 119, 323, 645
AlwNI CAGNNNCTG 1 cut(s) 475
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 2 cut(s) 414, 671
ApoI RAATTY 1 cut(s) 176
AsiGI ACCGGT 1 cut(s) 379
Asp700I GAANNNNTTC 1 cut(s) 156
Asp718I GGTACC 2 cut(s) 376, 465
AsuC2I CCSGG 2 cut(s) 312, 313
AsuHPI GGTGA 2 cut(s) 122, 590
AvaI CYCGRG 1 cut(s) 311
BanI GGYRCC 2 cut(s) 376, 465
BanII GRGCYC 2 cut(s) 13, 171
BbsI GAAGAC 1 cut(s) 580
Bbv12I GWGCWC 1 cut(s) 13
BccI CCATC 1 cut(s) 239
BcnI CCSGG 2 cut(s) 312, 313
BfaI CTAG 1 cut(s) 149
BlpI GCTNAGC 1 cut(s) 365
Bme1390I CCNGG 2 cut(s) 312, 313
BmeT110I CYCGRG 1 cut(s) 311
BmiI GGNNCC 3 cut(s) 378, 467, 614
BmrFI CCNGG 2 cut(s) 312, 313
BmrI ACTGGG 1 cut(s) 612
BmsI GCATC 1 cut(s) 106
BmuI ACTGGG 1 cut(s) 612
BpiI GAAGAC 1 cut(s) 580
Bpu1102I GCTNAGC 1 cut(s) 365
BpuEI CTTGAG 1 cut(s) 418
BpuMI CCSGG 2 cut(s) 312, 313
Bsa29I ATCGAT 2 cut(s) 35, 318
BsaHI GRCGYC 2 cut(s) 358, 452
BsaJI CCNNGG 2 cut(s) 311, 461
BsaWI WCCGGW 1 cut(s) 379
BsaXI ACNNNNNCTCC 7 cut(s) 144, 174, 442, 472, 596, 626, 651
Bse118I RCCGGY 1 cut(s) 379
Bse1I ACTGG 3 cut(s) 333, 378, 618
BseCI ATCGAT 2 cut(s) 35, 318
BseDI CCNNGG 2 cut(s) 311, 461
BseGI GGATG 1 cut(s) 528
BseMII CTCAG 2 cut(s) 356, 618
BseNI ACTGG 3 cut(s) 333, 378, 618
BseRI GAGGAG 1 cut(s) 675
BseYI CCCAGC 2 cut(s) 144, 439
BsgI GTGCAG 1 cut(s) 34
BshFI GGCC 2 cut(s) 416, 673
BshNI GGYRCC 2 cut(s) 376, 465
BshTI ACCGGT 1 cut(s) 379
BshVI ATCGAT 2 cut(s) 35, 318
BsiHKAI GWGCWC 1 cut(s) 13
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 3 cut(s) 312, 380, 417
BslFI GGGAC 1 cut(s) 418
BsmFI GGGAC 1 cut(s) 418
BsnI GGCC 2 cut(s) 416, 673
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 13, 171
Bsp1407I TGTACA 1 cut(s) 248
Bsp143I GATC 5 cut(s) 4, 36, 124, 315, 637
Bsp1720I GCTNAGC 1 cut(s) 365
BspANI GGCC 2 cut(s) 416, 673
BspCNI CTCAG 2 cut(s) 357, 619
BspDI ATCGAT 2 cut(s) 35, 318
BspLI GGNNCC 3 cut(s) 378, 467, 614
BspPI GGATC 4 cut(s) 12, 119, 323, 645
BspT107I GGYRCC 2 cut(s) 376, 465
BsrFI RCCGGY 1 cut(s) 379
BsrGI TGTACA 1 cut(s) 248
BsrI ACTGG 3 cut(s) 333, 378, 618
BssAI RCCGGY 1 cut(s) 379
BssECI CCNNGG 2 cut(s) 311, 461
BssMI GATC 5 cut(s) 4, 36, 124, 315, 637
BssNI GRCGYC 2 cut(s) 358, 452
BssT1I CCWWGG 1 cut(s) 461
Bst6I CTCTTC 1 cut(s) 114
BstACI GRCGYC 2 cut(s) 358, 452
BstAUI TGTACA 1 cut(s) 248
BstC8I GCNNGC 3 cut(s) 362, 480, 505
BstDEI CTNAG 3 cut(s) 365, 627, 675
BstF5I GGATG 1 cut(s) 528
BstKTI GATC 5 cut(s) 7, 39, 127, 318, 640
BstMBI GATC 5 cut(s) 4, 36, 124, 315, 637
BstMWI GCNNNNNNNGC 4 cut(s) 154, 225, 366, 604
BstNSI RCATGY 2 cut(s) 295, 428
BstSCI CCNGG 2 cut(s) 310, 311
BstV2I GAAGAC 1 cut(s) 580
BstX2I RGATCY 1 cut(s) 637
BstXI CCANNNNNNTGG 1 cut(s) 462
BstYI RGATCY 1 cut(s) 637
Bsu15I ATCGAT 2 cut(s) 35, 318
BsuRI GGCC 2 cut(s) 416, 673
BsuTUI ATCGAT 2 cut(s) 35, 318
BtsCI GGATG 1 cut(s) 528
BtsIMutI CAGTG 2 cut(s) 51, 625
Cac8I GCNNGC 3 cut(s) 362, 480, 505
CaiI CAGNNNCTG 1 cut(s) 475
Cfr10I RCCGGY 1 cut(s) 379
Cfr9I CCCGGG 1 cut(s) 311
ClaI ATCGAT 2 cut(s) 35, 318
CseI GACGC 2 cut(s) 366, 460
Csp6I GTAC 3 cut(s) 249, 377, 466
CspAI ACCGGT 1 cut(s) 379
CviAII CATG 2 cut(s) 292, 425
CviQI GTAC 3 cut(s) 249, 377, 466
DdeI CTNAG 3 cut(s) 365, 627, 675
DpnI GATC 5 cut(s) 6, 38, 126, 317, 639
DpnII GATC 5 cut(s) 4, 36, 124, 315, 637
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 461
Eco24I GRGCYC 2 cut(s) 13, 171
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 620
Eco88I CYCGRG 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 11
EcoT14I CCWWGG 1 cut(s) 461
EcoT38I GRGCYC 2 cut(s) 13, 171
ErhI CCWWGG 1 cut(s) 461
FaeI CATG 2 cut(s) 295, 428
FaiI YATR 4 cut(s) 293, 410, 426, 500
FaqI GGGAC 1 cut(s) 418
FatI CATG 2 cut(s) 291, 424
FokI GGATG 1 cut(s) 515
FriOI GRGCYC 2 cut(s) 13, 171
FspBI CTAG 1 cut(s) 149
GsaI CCCAGC 2 cut(s) 148, 443
HaeIII GGCC 2 cut(s) 416, 673
HapII CCGG 3 cut(s) 312, 380, 417
HgaI GACGC 2 cut(s) 366, 460
Hin1I GRCGYC 2 cut(s) 358, 452
Hin1II CATG 2 cut(s) 295, 428
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 1 cut(s) 396
HpaII CCGG 3 cut(s) 312, 380, 417
HphI GGTGA 2 cut(s) 122, 590
Hpy188I TCNGA 1 cut(s) 647
Hpy188III TCNNGA 3 cut(s) 113, 403, 592
Hpy99I CGWCG 2 cut(s) 454, 647
HpyAV CCTTC 4 cut(s) 359, 547, 551, 595
HpyCH4V TGCA 3 cut(s) 51, 275, 478
HpyF10VI GCNNNNNNNGC 4 cut(s) 154, 225, 366, 604
HpyF3I CTNAG 3 cut(s) 365, 627, 675
Hsp92I GRCGYC 2 cut(s) 358, 452
Hsp92II CATG 2 cut(s) 295, 428
KpnI GGTACC 2 cut(s) 380, 469
Kzo9I GATC 5 cut(s) 4, 36, 124, 315, 637
LmnI GCTCC 2 cut(s) 554, 604
LweI GCATC 1 cut(s) 106
MaeI CTAG 1 cut(s) 149
MaeIII GTNAC 1 cut(s) 298
MalI GATC 5 cut(s) 6, 38, 126, 317, 639
MboI GATC 5 cut(s) 4, 36, 124, 315, 637
MboII GAAGA 3 cut(s) 31, 101, 580
MflI RGATCY 1 cut(s) 637
MhlI GDGCHC 2 cut(s) 13, 171
MluCI AATT 2 cut(s) 176, 581
MmeI TCCRAC 1 cut(s) 625
MroXI GAANNNNTTC 1 cut(s) 156
MseI TTAA 1 cut(s) 63
MspI CCGG 3 cut(s) 312, 380, 417
MspR9I CCNGG 2 cut(s) 312, 313
MwoI GCNNNNNNNGC 4 cut(s) 154, 225, 366, 604
NciI CCSGG 2 cut(s) 312, 313
NdeII GATC 5 cut(s) 4, 36, 124, 315, 637
NlaIII CATG 2 cut(s) 295, 428
NlaIV GGNNCC 3 cut(s) 378, 467, 614
NspI RCATGY 2 cut(s) 295, 428
PciI ACATGT 1 cut(s) 424
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 1 cut(s) 396
PinAI ACCGGT 1 cut(s) 379
PscI ACATGT 1 cut(s) 424
Psp124BI GAGCTC 1 cut(s) 13
PspFI CCCAGC 2 cut(s) 144, 439
PspN4I GGNNCC 3 cut(s) 378, 467, 614
PsrI GAACNNNNNNTAC 4 cut(s) 241, 273, 369, 401
PstNI CAGNNNCTG 1 cut(s) 475
PsuI RGATCY 1 cut(s) 637
RsaI GTAC 3 cut(s) 250, 378, 467
RsaNI GTAC 3 cut(s) 249, 377, 466
SacI GAGCTC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 63
Sau3AI GATC 5 cut(s) 4, 36, 124, 315, 637
ScrFI CCNGG 2 cut(s) 312, 313
SduI GDGCHC 2 cut(s) 13, 171
SetI ASST 9 cut(s) 13, 85, 150, 159, 460, 491, 600, 614, 657
SfaNI GCATC 1 cut(s) 106
SmaI CCCGGG 1 cut(s) 313
SmlI CTYRAG 1 cut(s) 433
SmoI CTYRAG 1 cut(s) 433
Sse9I AATT 2 cut(s) 176, 581
SspI AATATT 1 cut(s) 238
SspMI CTAG 1 cut(s) 149
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 2 cut(s) 310, 311
StyI CCWWGG 1 cut(s) 461
TaqI TCGA 4 cut(s) 7, 35, 318, 593
TasI AATT 2 cut(s) 176, 581
TatI WGTACW 1 cut(s) 248
TfiI GAWTC 1 cut(s) 396
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 2 cut(s) 58, 625
TspDTI ATGAA 1 cut(s) 517
TspMI CCCGGG 1 cut(s) 311
TspRI CASTG 2 cut(s) 58, 625
XapI RAATTY 1 cut(s) 176
XceI RCATGY 2 cut(s) 295, 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 197
XmaI CCCGGG 1 cut(s) 311
XmnI GAANNNNTTC 1 cut(s) 156
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.