Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003132

Physical Location & Seq

Genomic Coordinates Reverse (-)

25072667 .. 25076214

Gene Structure 3548 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1160300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2271 bp

ATGGACATGGATATCGACGAAACAGCCAAAGGGGAAGAAAAGCAAGGAAAGCCCGTCGTAGTAATATTGGTGGGTGCGCCCGGAAGCGGAAAGTCCACCTTCTGTGAGCATGTCATGCGCTCCTCCTCCGCTCGCCCCTGGGTTCGCGTTTGCCAGGATACTATAAAAAATGGTAAAGCGGGAACGAAAGCGCAGTGTATTGAGAGTGCAATGAATGCATTGAAGGATGGGAAGAGTGTTTTTATAGATAGGTGTAATCTGGAGAAAGAACAAAGGGATGAGTTTGTGAAGCTTGGTGGTAGTACCCAGGTTGATGTTCATGCTGTGGTGCTTGATCTTCCTGCCAAGCTTTGTATATCGCGGTCTGTGAAGAGAACAGGCCACGAGGGAAACTTGCAAGGTGGGAGAGCTGCTGCGGTTGTGAATAGAATGCTGCAGAAGAAAGAACTGCCTAAACTGAGTGAAGGGTTTGCTAGAATCACTTGTTGCCAGAGTGAGAGTGATGTTCAATCTGCCGTTGATGCATATAGTGGACTTGGTCCGTTGGATACACTTCCAAGTGGTTATTTTGGCCAGAAGAACACCGGTGCGAAAGTACAACTAGGTATAATGAGGTTTTTAAAGAAAACAGATGGTCCTGCTAATACTGAATCCACTTCGAAGAGTGTACCAGATTCGAATGCTTCTCAAATTACTGAAGAAAAGGAAACCTCTTCAAAGGGAACAGGTTCATTGTCTGAAAATTCTCGTAAGGAGTCAAAGGAAGGTGCAGAGCTATTTGTAGGCTCTGTTGGCGGTGATGTTTCTCTAAACGATTCCCCCACTCTCGCATTTCCCTCCATATCGACAGCAGATTTTCAATTTGACATTGAGAAGGCGTCTGATATTATTGTCGAAAAAGTTATAGAATTTGTGAATAAGCTTGGAAATGCTAGACTTGTTTTAGTGGACTTGAGTCATAAATCAAAGATTTTGTCTTTGGTAAGGGCTAAAGCTTCAGAGAAAAACATTGACTCCAACAAGTTCTTCACATTTGTGGGAGATATCACTAAGCTTCATTCAGGAGGAGGTTTACACTGCAATGTAATAGCTAATGCTGCTAATTGGCGTCTTAAACCTGGAGGTGGAGGTGTCAATGCTGCAATCTTCAATGCTGGAGGTCCATCTTTGGAGGTTGCAACCAAAGAACAAGCCCAATCTCTGTATCCTGGGAGTGCCGTGGTTGTGCCTGTCCCTGCAACTTCTCCCTTGTTTAGTAGGGAAGGGGTAACCCATGTGATACATGTTGTTGGACCCAACATGAACCCACACAGACCAAATTGTCTGAACAATGACTATAGTAAAGGCTGCAAAGTTCTCCGGGAGGCTTACACTTCACTTTTCGAAGGTTTCGCAACAATTGTGAGGACCCAAGCAAAGTCACCCAAGGGAAGCATTGAGAACTTGCAGGCAAAGCTGCCAGAGTCACAAGAATATTCTGACAGTGCCTCTAGAAATCATTTTACAAACAGCAATCAAAAGACTAAGAGAGAGGATCCTCATAAATATGAGAGGAGCAAAAGAAGTAAAGCATATCAGGATGATACTGAAGATTCTAACACTGGTAAACCAGACCTGAGCAACAAGTCCAGTGGAAGCAGGACTAAATCCTGGGGGTCATGGGCTCAGGCTCTCTACAACACTGCTATGCACCCAGAGAAGCACAAGGATGCAGTACTAGAGATATCAGATGATGTCGTTGTGCTGAATGATCTTTATCCAAAGGCAAAAAGGCATGTTTTGGTGGTGGCACAATGTGAAGGTCTTGATCGACTGTCAGATGTTCATAAAGAACACCTTCCATTGTTAAGGACAATGCATGAAGTTGGTCTGAAGTGGGTTGAGAAATTCTTGCATGATGATTCGTCTTTGGTCTTCCGTCTTGGATATCACTTGGATCCCTCAATGCGACAGCTGCATCTGCATGTTATCAGCCAGGATTTTGATTCAACACACTTGAAGAATAAGAAACATTGGAACTCCTTCAATACTGCTTTCTTTCGAGACTCCGTGGATGTGGTAGAGGAAGTTAGTAGTGATGGAAAAGCAAAGCTAAAAGATGACGATAGCCTATTGTCTATGGAGTTGCGATGCCACCGATGTAGAAGCGCTCACCCCAACATACCCCGATTAAAATCTCACATTACTAACTGTCGAGCCACCTTTCCAAGCACCCTGCTCCAAAATGGTCGTCTTATTCACGCACCAAGCAATGTCAGCATTGGTCCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006139 GO:0006163 GO:0006725 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009117 GO:0009150 GO:0009259 GO:0009987 GO:0016787 GO:0016817 GO:0016819 GO:0019637 GO:0019693 GO:0034641 GO:0044237 GO:0044238 GO:0044281 GO:0046483 GO:0047627 GO:0055086 GO:0071704 GO:0072521 GO:1901135 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA_33 DcpS_C HLH Macro zf-C2HE

Protein Analysis

757

Amino Acids

82.72

Weight (kDa)

8.69

Isoelectric Point (pI)

40.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA_33	PF13671	20 - 149	4.5e-14	AAA domain
AAA_18	PF13238	21 - 135	1e-05	AAA domain
Macro	PF01661	365 - 441	8.2e-07	Macro domain
DcpS_C	PF11969	559 - 671	6.7e-24	Scavenger mRNA decapping enzyme C-term binding
HIT	PF01230	570 - 660	8.4e-09	HIT domain
zf-C2HE	PF16278	676 - 730	2.2e-10	C2HE / C2H2 / C2HC zinc-binding finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003132)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01310
fragaria_vesca	FvH4_6g11090 FvH4_6g11090 FvH4_6g11090 FvH4_6g11090 FvH4_6g11090
malus_domestica	MD04G1160300.v1.1 MD05G1095500.v1.1 MD10G1110900.v1.1 MD12G1172800.v1.1
prunus_persica	Prupe.6G280900_v2.0.a1 Prupe.6G280900_v2.0.a1 Prupe.6G280900_v2.0.a1 Prupe.6G280900_v2.0.a1 Prupe.6G280900_v2.0.a1
pyrus_communis	pycom04g14360 pycom12g16400 pycom12g16440
rosa_chinensis	RchiOBHm_Chr3g0462501 RchiOBHm_Chr3g0462521
rosa_laevigata	RLG00000024853 RLG00000024857
rosa_multiflora	Rmu_sc0003624.1_g000009 Rmu_sc0003624.1_g000012 Rmu_sc0003624.1_g000015 Rmu_sc0011346.1_g000002 Rmu_sc0011346.1_g000005
rosa_roxburghii	Rroxscaffold_6G00417460
rosa_rugosa	Rorug03G0057000
rosa_samantha	Rh3AG115200 Rh3BG118500 Rh3BG118600 Rh3CG121400 Rh3CG122000 Rh3DG120700
rosa_wichuraiana	Rw0G003810 Rw3G009750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1320
AccBSI	CCGCTC	1 cut(s)	131
AccII	CGCG	2 cut(s)	147, 361
AciI	CCGC	6 cut(s)	87, 129, 179, 361, 416, 795
AclWI	GGATC	4 cut(s)	1529, 1542, 1931, 1944
AcoI	YGGCCR	1 cut(s)	571
AcsI	RAATTY	3 cut(s)	742, 908, 1886
AcuI	CTGAAG	4 cut(s)	717, 981, 1608, 1892
AcyI	GRCGYC	2 cut(s)	878, 1108
AdeI	CACNNNGTG	1 cut(s)	1796
AfaI	GTAC	4 cut(s)	304, 597, 669, 1716
AfeI	AGCGCT	1 cut(s)	2149
AfiI	CCNNNNNNNGG	2 cut(s)	86, 1124
AflIII	ACRYGT	1 cut(s)	1282
AgeI	ACCGGT	1 cut(s)	584
AgsI	TTSAA	8 cut(s)	223, 509, 717, 860, 1150, 1989, 1999, 2026
AjnI	CCWGG	7 cut(s)	137, 153, 306, 1117, 1207, 1649, 1974
AjuI	GAANNNNNNNTTGG	2 cut(s)	1010, 1042
AleI	CACNNNNGTG	1 cut(s)	1034
Alw26I	GTCTC	1 cut(s)	2037
AlwI	GGATC	4 cut(s)	1529, 1542, 1931, 1944
Aor51HI	AGCGCT	1 cut(s)	2149
AoxI	GGCC	2 cut(s)	379, 571
ApeKI	GCWGC	8 cut(s)	410, 413, 433, 1097, 1139, 1347, 1456, 1954
ApoI	RAATTY	3 cut(s)	742, 908, 1886
ArsI	GACNNNNNNTTYG	4 cut(s)	959, 991, 2215, 2247
AsiGI	ACCGGT	1 cut(s)	584
Asp700I	GAANNNNTTC	3 cut(s)	727, 1836, 2021
AspLEI	GCGC	4 cut(s)	79, 120, 193, 2150
AspS9I	GGNCC	6 cut(s)	539, 635, 1160, 1292, 1407, 2264
AsuC2I	CCSGG	2 cut(s)	81, 1361
AsuHPI	GGTGA	3 cut(s)	809, 1413, 2144
AsuII	TTCGAA	3 cut(s)	659, 677, 1383
AvaII	GGWCC	6 cut(s)	539, 635, 1160, 1292, 1407, 2264
BalI	TGGCCA	1 cut(s)	573
BamHI	GGATCC	2 cut(s)	1534, 1936
BanII	GRGCYC	1 cut(s)	1666
BauI	CACGAG	1 cut(s)	383
BbsI	GAAGAC	1 cut(s)	1906
BbvI	GCAGC	8 cut(s)	397, 400, 420, 1084, 1126, 1334, 1443, 1941
BccI	CCATC	4 cut(s)	221, 626, 1171, 2072
BceAI	ACGGC	2 cut(s)	500, 1202
BciT130I	CCWGG	7 cut(s)	139, 155, 308, 1119, 1209, 1651, 1976
BciVI	GTATCC	3 cut(s)	151, 541, 1215
BcnI	CCSGG	2 cut(s)	81, 1361
BcoDI	GTCTC	1 cut(s)	2037
BfaI	CTAG	5 cut(s)	474, 602, 933, 1491, 1718
BfmI	CTRYAG	2 cut(s)	434, 1336
BfoI	RGCGCY	1 cut(s)	2151
BfuI	GTATCC	3 cut(s)	151, 541, 1215
BisI	GCNGC	8 cut(s)	411, 414, 434, 1098, 1140, 1348, 1457, 1955
BlsI	GCNGC	8 cut(s)	412, 415, 435, 1099, 1141, 1349, 1458, 1956
BmcAI	AGTACT	1 cut(s)	1716
Bme1390I	CCNGG	9 cut(s)	81, 139, 155, 308, 1119, 1209, 1361, 1651, 1976
Bme18I	GGWCC	6 cut(s)	539, 635, 1160, 1292, 1407, 2264
BmgT120I	GGNCC	6 cut(s)	539, 635, 1160, 1292, 1407, 2264
BmiI	GGNNCC	4 cut(s)	1294, 1409, 1536, 1938
BmrFI	CCNGG	9 cut(s)	81, 139, 155, 308, 1119, 1209, 1361, 1651, 1976
BmsI	GCATC	4 cut(s)	511, 1699, 1966, 2120
BoxI	GACNNNNGTC	1 cut(s)	954
BpiI	GAAGAC	1 cut(s)	1906
BpmI	CTGGAG	3 cut(s)	281, 1140, 1176
Bpu10I	CCTNAGC	2 cut(s)	1616, 1665
Bpu14I	TTCGAA	3 cut(s)	659, 677, 1383
BpuEI	CTTGAG	1 cut(s)	973
BpuMI	CCSGG	2 cut(s)	81, 1361
BsaBI	GATNNNNATC	2 cut(s)	1755, 2173
BsaHI	GRCGYC	2 cut(s)	878, 1108
BsaJI	CCNNGG	8 cut(s)	137, 138, 306, 1208, 1218, 1425, 1650, 2049
BsaWI	WCCGGW	1 cut(s)	584
BsaXI	ACNNNNNCTCC	2 cut(s)	998, 1028
Bsc4I	CCNNNNNNNGG	2 cut(s)	86, 1124
Bse118I	RCCGGY	1 cut(s)	584
Bse1I	ACTGG	2 cut(s)	1606, 1629
Bse3DI	GCAATG	3 cut(s)	216, 1087, 2257
Bse8I	GATNNNNATC	2 cut(s)	1755, 2173
BseBI	CCWGG	7 cut(s)	139, 155, 308, 1119, 1209, 1651, 1976
BseDI	CCNNGG	8 cut(s)	137, 138, 306, 1208, 1218, 1425, 1650, 2049
BseGI	GGATG	5 cut(s)	232, 283, 1585, 1714, 2059
BseJI	GATNNNNATC	2 cut(s)	1755, 2173
BseLI	CCNNNNNNNGG	2 cut(s)	86, 1124
BseMI	GCAATG	3 cut(s)	216, 1087, 2257
BseMII	CTCAG	3 cut(s)	449, 1607, 1679
BseNI	ACTGG	2 cut(s)	1606, 1629
BseRI	GAGGAG	4 cut(s)	112, 115, 1080, 1567
BseXI	GCAGC	8 cut(s)	397, 400, 420, 1084, 1126, 1334, 1443, 1941
BsgI	GTGCAG	1 cut(s)	789
Bsh1236I	CGCG	2 cut(s)	147, 361
BshFI	GGCC	2 cut(s)	381, 573
BshTI	ACCGGT	1 cut(s)	584
BsiSI	CCGG	3 cut(s)	81, 585, 1360
BslFI	GGGAC	1 cut(s)	1217
BslI	CCNNNNNNNGG	2 cut(s)	86, 1124
BsmAI	GTCTC	1 cut(s)	2037
BsmFI	GGGAC	1 cut(s)	1217
BsmI	GAATGC	3 cut(s)	220, 435, 685
BsnI	GGCC	2 cut(s)	381, 573
Bsp119I	TTCGAA	3 cut(s)	659, 677, 1383
Bsp1286I	GDGCHC	1 cut(s)	1666
Bsp143I	GATC	5 cut(s)	334, 1534, 1750, 1807, 1936
BspACI	CCGC	6 cut(s)	87, 129, 179, 361, 416, 795
BspANI	GGCC	2 cut(s)	381, 573
BspCNI	CTCAG	3 cut(s)	450, 1608, 1678
BspFNI	CGCG	2 cut(s)	147, 361
BspLI	GGNNCC	4 cut(s)	1294, 1409, 1536, 1938
BspMAI	CTGCAG	1 cut(s)	438
BspPI	GGATC	4 cut(s)	1529, 1542, 1931, 1944
BspT104I	TTCGAA	3 cut(s)	659, 677, 1383
BsrBI	CCGCTC	1 cut(s)	131
BsrDI	GCAATG	3 cut(s)	216, 1087, 2257
BsrFI	RCCGGY	1 cut(s)	584
BsrI	ACTGG	2 cut(s)	1606, 1629
BssAI	RCCGGY	1 cut(s)	584
BssECI	CCNNGG	8 cut(s)	137, 138, 306, 1208, 1218, 1425, 1650, 2049
BssMI	GATC	5 cut(s)	334, 1534, 1750, 1807, 1936
BssNI	GRCGYC	2 cut(s)	878, 1108
BssSI	CACGAG	1 cut(s)	383
BssT1I	CCWWGG	1 cut(s)	1425
Bst2BI	CACGAG	1 cut(s)	383
Bst2UI	CCWGG	7 cut(s)	139, 155, 308, 1119, 1209, 1651, 1976
Bst4CI	ACNGT	3 cut(s)	1484, 1815, 2192
Bst6I	CTCTTC	4 cut(s)	227, 365, 656, 718
BstACI	GRCGYC	2 cut(s)	878, 1108
BstAPI	GCANNNNNTGC	2 cut(s)	115, 215
BstBI	TTCGAA	3 cut(s)	659, 677, 1383
BstC8I	GCNNGC	2 cut(s)	133, 1449
BstDEI	CTNAG	5 cut(s)	458, 1050, 1524, 1616, 1665
BstDSI	CCRYGG	2 cut(s)	1218, 2049
BstEII	GGTNACC	1 cut(s)	1267
BstF5I	GGATG	5 cut(s)	232, 283, 1585, 1714, 2059
BstFNI	CGCG	2 cut(s)	147, 361
BstH2I	RGCGCY	1 cut(s)	2151
BstHHI	GCGC	4 cut(s)	79, 120, 193, 2150
BstKTI	GATC	5 cut(s)	337, 1537, 1753, 1810, 1939
BstMAI	GTCTC	1 cut(s)	2037
BstMBI	GATC	5 cut(s)	334, 1534, 1750, 1807, 1936
BstNI	CCWGG	7 cut(s)	139, 155, 308, 1119, 1209, 1651, 1976
BstNSI	RCATGY	4 cut(s)	113, 1286, 1778, 1967
BstPAI	GACNNNNGTC	1 cut(s)	954
BstPI	GGTNACC	1 cut(s)	1267
BstSCI	CCNGG	9 cut(s)	79, 137, 153, 306, 1117, 1207, 1359, 1649, 1974
BstSFI	CTRYAG	2 cut(s)	434, 1336
BstUI	CGCG	2 cut(s)	147, 361
BstV1I	GCAGC	8 cut(s)	397, 400, 420, 1084, 1126, 1334, 1443, 1941
BstV2I	GAAGAC	1 cut(s)	1906
BstX2I	RGATCY	2 cut(s)	1534, 1936
BstYI	RGATCY	2 cut(s)	1534, 1936
BsuI	GTATCC	3 cut(s)	151, 541, 1215
BsuRI	GGCC	2 cut(s)	381, 573
BtgI	CCRYGG	2 cut(s)	1218, 2049
BtgZI	GCGATG	1 cut(s)	2143
BtsCI	GGATG	5 cut(s)	232, 283, 1585, 1714, 2059
BtsI	GCAGTG	3 cut(s)	200, 1075, 1680
BtsIMutI	CAGTG	6 cut(s)	200, 1075, 1489, 1599, 1636, 1680
Cac8I	GCNNGC	2 cut(s)	133, 1449
CfoI	GCGC	4 cut(s)	79, 120, 193, 2150
Cfr10I	RCCGGY	1 cut(s)	584
Cfr13I	GGNCC	6 cut(s)	539, 635, 1160, 1292, 1407, 2264
CseI	GACGC	2 cut(s)	867, 1097
Csp6I	GTAC	4 cut(s)	303, 596, 668, 1715
CspAI	ACCGGT	1 cut(s)	584
CspCI	CAANNNNNGTGG	2 cut(s)	1612, 1647
CviQI	GTAC	4 cut(s)	303, 596, 668, 1715
DdeI	CTNAG	5 cut(s)	458, 1050, 1524, 1616, 1665
DpnI	GATC	5 cut(s)	336, 1536, 1752, 1809, 1938
DpnII	GATC	5 cut(s)	334, 1534, 1750, 1807, 1936
DraI	TTTAAA	1 cut(s)	621
DraIII	CACNNNGTG	1 cut(s)	1796
DrdI	GACNNNNNNGTC	1 cut(s)	1320
DseDI	GACNNNNNNGTC	1 cut(s)	1320
EaeI	YGGCCR	1 cut(s)	571
Eam1104I	CTCTTC	4 cut(s)	227, 365, 656, 718
EarI	CTCTTC	4 cut(s)	227, 365, 656, 718
Eco130I	CCWWGG	1 cut(s)	1425
Eco24I	GRGCYC	1 cut(s)	1666
Eco32I	GATATC	4 cut(s)	13, 1045, 1725, 1928
Eco47I	GGWCC	6 cut(s)	539, 635, 1160, 1292, 1407, 2264
Eco47III	AGCGCT	1 cut(s)	2149
Eco57I	CTGAAG	4 cut(s)	717, 981, 1608, 1892
Eco91I	GGTNACC	1 cut(s)	1267
EcoO109I	RGGNCCY	1 cut(s)	1407
EcoO65I	GGTNACC	1 cut(s)	1267
EcoRII	CCWGG	7 cut(s)	137, 153, 306, 1117, 1207, 1649, 1974
EcoRV	GATATC	4 cut(s)	13, 1045, 1725, 1928
EcoT14I	CCWWGG	1 cut(s)	1425
EcoT22I	ATGCAT	3 cut(s)	220, 526, 1860
EcoT38I	GRGCYC	1 cut(s)	1666
ErhI	CCWWGG	1 cut(s)	1425
FalI	AAGNNNNNCTT	4 cut(s)	83, 115, 1821, 1853
FaqI	GGGAC	1 cut(s)	1217
FauI	CCCGC	1 cut(s)	172
Fnu4HI	GCNGC	8 cut(s)	411, 414, 434, 1098, 1140, 1348, 1457, 1955
FokI	GGATG	5 cut(s)	239, 290, 1592, 1721, 2066
FriOI	GRGCYC	1 cut(s)	1666
Fsp4HI	GCNGC	8 cut(s)	411, 414, 434, 1098, 1140, 1348, 1457, 1955
FspBI	CTAG	5 cut(s)	474, 602, 933, 1491, 1718
GlaI	GCGC	4 cut(s)	78, 119, 192, 2149
GluI	GCNGC	8 cut(s)	411, 414, 434, 1098, 1140, 1348, 1457, 1955
GsuI	CTGGAG	3 cut(s)	281, 1140, 1176
HaeII	RGCGCY	1 cut(s)	2151
HaeIII	GGCC	2 cut(s)	381, 573
HapII	CCGG	3 cut(s)	81, 585, 1360
HgaI	GACGC	2 cut(s)	867, 1097
HhaI	GCGC	4 cut(s)	79, 120, 193, 2150
Hin1I	GRCGYC	2 cut(s)	878, 1108
Hin6I	GCGC	4 cut(s)	77, 118, 191, 2148
HinP1I	GCGC	4 cut(s)	77, 118, 191, 2148
HindIII	AAGCTT	5 cut(s)	290, 347, 920, 993, 1052
HpaII	CCGG	3 cut(s)	81, 585, 1360
HphI	GGTGA	3 cut(s)	809, 1413, 2144
Hpy166II	GTNNAC	6 cut(s)	96, 533, 668, 949, 1073, 1607
Hpy188I	TCNGA	8 cut(s)	739, 883, 1000, 1326, 1480, 1729, 1819, 1872
Hpy188III	TCNNGA	6 cut(s)	260, 1062, 1491, 1577, 1805, 2042
Hpy8I	GTNNAC	6 cut(s)	96, 533, 668, 949, 1073, 1607
Hpy99I	CGWCG	2 cut(s)	20, 59
HpyCH4III	ACNGT	3 cut(s)	1484, 1815, 2192
HpyF3I	CTNAG	5 cut(s)	458, 1050, 1524, 1616, 1665
Hsp92I	GRCGYC	2 cut(s)	878, 1108
HspAI	GCGC	4 cut(s)	77, 118, 191, 2148
Kzo9I	GATC	5 cut(s)	334, 1534, 1750, 1807, 1936
LmnI	GCTCC	3 cut(s)	125, 1554, 2223
Lsp1109I	GCAGC	8 cut(s)	397, 400, 420, 1084, 1126, 1334, 1443, 1941
LweI	GCATC	4 cut(s)	511, 1699, 1966, 2120
MaeI	CTAG	5 cut(s)	474, 602, 933, 1491, 1718
MaeIII	GTNAC	3 cut(s)	1267, 1419, 1464
MalI	GATC	5 cut(s)	336, 1536, 1752, 1809, 1938
MbiI	CCGCTC	1 cut(s)	131
MboI	GATC	5 cut(s)	334, 1534, 1750, 1807, 1936
MfeI	CAATTG	1 cut(s)	1398
MflI	RGATCY	2 cut(s)	1534, 1936
MhlI	GDGCHC	1 cut(s)	1666
MlsI	TGGCCA	1 cut(s)	573
MluCI	AATT	8 cut(s)	690, 742, 860, 908, 1102, 1318, 1398, 1886
MluNI	TGGCCA	1 cut(s)	573
MlyI	GAGTC	5 cut(s)	764, 964, 1007, 1472, 2039
MmeI	TCCRAC	3 cut(s)	525, 1041, 1270
Mox20I	TGGCCA	1 cut(s)	573
Mph1103I	ATGCAT	3 cut(s)	220, 526, 1860
MroXI	GAANNNNTTC	3 cut(s)	727, 1836, 2021
MscI	TGGCCA	1 cut(s)	573
MseI	TTAA	4 cut(s)	620, 1113, 1847, 2171
MslI	CAYNNNNRTG	6 cut(s)	1034, 1080, 1545, 1685, 1707, 1962
Msp20I	TGGCCA	1 cut(s)	573
MspA1I	CMGCKG	1 cut(s)	1954
MspI	CCGG	3 cut(s)	81, 585, 1360
MspR9I	CCNGG	9 cut(s)	81, 139, 155, 308, 1119, 1209, 1361, 1651, 1976
MunI	CAATTG	1 cut(s)	1398
Mva1269I	GAATGC	3 cut(s)	220, 435, 685
MvaI	CCWGG	7 cut(s)	139, 155, 308, 1119, 1209, 1651, 1976
MvnI	CGCG	2 cut(s)	147, 361
NciI	CCSGG	2 cut(s)	81, 1361
NdeII	GATC	5 cut(s)	334, 1534, 1750, 1807, 1936
NlaIV	GGNNCC	4 cut(s)	1294, 1409, 1536, 1938
NmuCI	GTSAC	2 cut(s)	1419, 1464
NsiI	ATGCAT	3 cut(s)	220, 526, 1860
NspI	RCATGY	4 cut(s)	113, 1286, 1778, 1967
NspV	TTCGAA	3 cut(s)	659, 677, 1383
OliI	CACNNNNGTG	1 cut(s)	1034
PasI	CCCWGGG	1 cut(s)	138
PciI	ACATGT	1 cut(s)	1282
PctI	GAATGC	3 cut(s)	220, 435, 685
PdmI	GAANNNNTTC	3 cut(s)	727, 1836, 2021
PfeI	GAWTC	7 cut(s)	477, 650, 674, 815, 1592, 1901, 1985
PflFI	GACNNNGTC	1 cut(s)	537
PfoI	TCCNGGA	1 cut(s)	1359
PinAI	ACCGGT	1 cut(s)	584
PkrI	GCNGC	8 cut(s)	412, 415, 435, 1099, 1141, 1349, 1458, 1956
PleI	GAGTC	5 cut(s)	763, 963, 1007, 1471, 2039
PpsI	GAGTC	5 cut(s)	763, 963, 1007, 1471, 2039
PpuMI	RGGWCCY	1 cut(s)	1407
PscI	ACATGT	1 cut(s)	1282
PshAI	GACNNNNGTC	1 cut(s)	954
Psp5II	RGGWCCY	1 cut(s)	1407
Psp6I	CCWGG	7 cut(s)	137, 153, 306, 1117, 1207, 1649, 1974
PspEI	GGTNACC	1 cut(s)	1267
PspGI	CCWGG	7 cut(s)	137, 153, 306, 1117, 1207, 1649, 1974
PspN4I	GGNNCC	4 cut(s)	1294, 1409, 1536, 1938
PspPI	GGNCC	6 cut(s)	539, 635, 1160, 1292, 1407, 2264
PspPPI	RGGWCCY	1 cut(s)	1407
PstI	CTGCAG	1 cut(s)	438
PsuI	RGATCY	2 cut(s)	1534, 1936
PsyI	GACNNNGTC	1 cut(s)	537
PvuII	CAGCTG	1 cut(s)	1954
RsaI	GTAC	4 cut(s)	304, 597, 669, 1716
RsaNI	GTAC	4 cut(s)	303, 596, 668, 1715
RseI	CAYNNNNRTG	6 cut(s)	1034, 1080, 1545, 1685, 1707, 1962
SaqAI	TTAA	4 cut(s)	620, 1113, 1847, 2171
SatI	GCNGC	8 cut(s)	411, 414, 434, 1098, 1140, 1348, 1457, 1955
Sau3AI	GATC	5 cut(s)	334, 1534, 1750, 1807, 1936
Sau96I	GGNCC	6 cut(s)	539, 635, 1160, 1292, 1407, 2264
ScaI	AGTACT	1 cut(s)	1716
SchI	GAGTC	5 cut(s)	764, 964, 1007, 1472, 2039
ScrFI	CCNGG	9 cut(s)	81, 139, 155, 308, 1119, 1209, 1361, 1651, 1976
SduI	GDGCHC	1 cut(s)	1666
SfaNI	GCATC	4 cut(s)	511, 1699, 1966, 2120
SfcI	CTRYAG	2 cut(s)	434, 1336
SfuI	TTCGAA	3 cut(s)	659, 677, 1383
SgrAI	CRCCGGYG	1 cut(s)	584
SinI	GGWCC	6 cut(s)	539, 635, 1160, 1292, 1407, 2264
SmiMI	CAYNNNNRTG	6 cut(s)	1034, 1080, 1545, 1685, 1707, 1962
SmlI	CTYRAG	1 cut(s)	952
SmoI	CTYRAG	1 cut(s)	952
Sse9I	AATT	8 cut(s)	690, 742, 860, 908, 1102, 1318, 1398, 1886
SsiI	CCGC	6 cut(s)	87, 129, 179, 361, 416, 795
SspI	AATATT	2 cut(s)	66, 1475
SspMI	CTAG	5 cut(s)	474, 602, 933, 1491, 1718
StyD4I	CCNGG	9 cut(s)	79, 137, 153, 306, 1117, 1207, 1359, 1649, 1974
StyI	CCWWGG	1 cut(s)	1425
TaaI	ACNGT	3 cut(s)	1484, 1815, 2192
TaqI	TCGA	9 cut(s)	15, 659, 677, 845, 894, 1383, 1810, 2041, 2194
TasI	AATT	8 cut(s)	690, 742, 860, 908, 1102, 1318, 1398, 1886
TatI	WGTACW	2 cut(s)	595, 1714
TfiI	GAWTC	7 cut(s)	477, 650, 674, 815, 1592, 1901, 1985
Tru1I	TTAA	4 cut(s)	620, 1113, 1847, 2171
Tru9I	TTAA	4 cut(s)	620, 1113, 1847, 2171
TscAI	CASTG	6 cut(s)	200, 1082, 1489, 1606, 1636, 1687
TseFI	GTSAC	2 cut(s)	1419, 1464
TseI	GCWGC	8 cut(s)	410, 413, 433, 1097, 1139, 1347, 1456, 1954
Tsp45I	GTSAC	2 cut(s)	1419, 1464
TspDTI	ATGAA	7 cut(s)	227, 308, 720, 1046, 1316, 1814, 1875
TspGWI	ACGGA	4 cut(s)	531, 1907, 2038, 2256
TspRI	CASTG	6 cut(s)	200, 1082, 1489, 1606, 1636, 1687
Tth111I	GACNNNGTC	1 cut(s)	537
VpaK11BI	GGWCC	6 cut(s)	539, 635, 1160, 1292, 1407, 2264
XapI	RAATTY	3 cut(s)	742, 908, 1886
XbaI	TCTAGA	1 cut(s)	1490
XceI	RCATGY	4 cut(s)	113, 1286, 1778, 1967
XmnI	GAANNNNTTC	3 cut(s)	727, 1836, 2021
XspI	CTAG	5 cut(s)	474, 602, 933, 1491, 1718
ZrmI	AGTACT	1 cut(s)	1716
Zsp2I	ATGCAT	3 cut(s)	220, 526, 1860

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.