RLG00000024857

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003132
Physical Location & Seq
Reverse (-)
39833160 .. 39834027
868 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024857

Sequence Viewer

Length: 603 bp
ATGGACATGGATGTTGATGAACCAACCAAAGAAAAGCAAGGAAAGCCCATAATCGTAGTATTGATGGGTGCACCCGGTAGTGGCAAATCCACATTCTGCGAACAAGTCATGGGCTCCTCTGTTCGTCCCTGGGTCCGTGTCTGCCAGATACTATCAAGAATGGGAAAGTTGGAAAGAAAGCTCAGTTTCTATAGTGCGAGGAGTGCATTGAGGGAAGGAAAGAGTGTGTTTATAGATAGATGTAATCTGGAGAAAGAACAAAGGGATGAGTTTGTGAAGCTGGGTGGTCCTCAGGTGGATGTTTATGCTGTGGTGCTTGATCTTCCTGCTAAGGTTTGTATATCGCAGTCGGTGAGGCGAACGGGACATGAGAGGAACTCGCAAGGCTTAAAAGCTGTGGCTGTTGTGAATAGAATGCTGCATAAGAAAGAATTGCCGAATCTGAGCGAAGGGTTTGGTAGGATCACTTTTTGCCAGATTGAGAGCTATGTTGAATCTGCCATTGGTACATATAGTGATATAGTGGACTCGGTCCGGTGGATACACTTCCCTATGGCACTTTTGGCCAGAAGAACCCGGGTGCAAAAGTTCAACCTGGTATAA

Protein Analysis

201

Amino Acids

22.55

Weight (kDa)

9.48

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_33 PF13671 19 - 141 3.8e-10 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 470
AcoI YGGCCR 1 cut(s) 564
AfaI GTAC 1 cut(s) 508
AfiI CCNNNNNNNGG 1 cut(s) 80
AgsI TTSAA 2 cut(s) 494, 592
AjnI CCWGG 2 cut(s) 128, 594
AjuI GAANNNNNNNTTGG 2 cut(s) 486, 518
AluBI AGCT 4 cut(s) 181, 280, 395, 486
AluI AGCT 4 cut(s) 181, 280, 395, 486
Alw21I GWGCWC 1 cut(s) 73
Alw44I GTGCAC 1 cut(s) 69
AlwI GGATC 1 cut(s) 470
Ama87I CYCGRG 1 cut(s) 576
AoxI GGCC 1 cut(s) 564
ApaLI GTGCAC 1 cut(s) 69
ApeKI GCWGC 1 cut(s) 418
AspS9I GGNCC 3 cut(s) 133, 287, 532
AsuC2I CCSGG 3 cut(s) 75, 577, 578
AsuHPI GGTGA 1 cut(s) 364
AvaI CYCGRG 1 cut(s) 576
AvaII GGWCC 3 cut(s) 133, 287, 532
AxyI CCTNAGG 1 cut(s) 291
BaeGI GKGCMC 1 cut(s) 73
BalI TGGCCA 1 cut(s) 566
BanII GRGCYC 1 cut(s) 116
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 1 cut(s) 405
BccI CCATC 1 cut(s) 58
BciT130I CCWGG 2 cut(s) 130, 596
BciVI GTATCC 1 cut(s) 534
BcnI CCSGG 3 cut(s) 75, 577, 578
BfmI CTRYAG 1 cut(s) 190
BfuI GTATCC 1 cut(s) 534
BisI GCNGC 1 cut(s) 419
BlsI GCNGC 1 cut(s) 420
Bme1390I CCNGG 5 cut(s) 75, 130, 577, 578, 596
Bme18I GGWCC 3 cut(s) 133, 287, 532
BmeT110I CYCGRG 1 cut(s) 576
BmgT120I GGNCC 3 cut(s) 133, 287, 532
BmiI GGNNCC 2 cut(s) 115, 134
BmrFI CCNGG 5 cut(s) 75, 130, 577, 578, 596
BplI GAGNNNNNCTC 2 cut(s) 362, 394
BpmI CTGGAG 1 cut(s) 269
Bpu10I CCTNAGC 1 cut(s) 330
BpuMI CCSGG 3 cut(s) 75, 577, 578
BsaJI CCNNGG 3 cut(s) 128, 129, 576
BsaWI WCCGGW 1 cut(s) 534
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse21I CCTNAGG 1 cut(s) 291
BseBI CCWGG 2 cut(s) 130, 596
BseDI CCNNGG 3 cut(s) 128, 129, 576
BseGI GGATG 3 cut(s) 16, 271, 304
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMII CTCAG 3 cut(s) 196, 305, 434
BseRI GAGGAG 2 cut(s) 106, 214
BseSI GKGCMC 1 cut(s) 73
BseXI GCAGC 1 cut(s) 405
BseYI CCCAGC 1 cut(s) 280
BshFI GGCC 1 cut(s) 566
BsiHKAI GWGCWC 1 cut(s) 73
BsiHKCI CYCGRG 1 cut(s) 576
BsiSI CCGG 3 cut(s) 75, 535, 577
BslFI GGGAC 2 cut(s) 111, 378
BslI CCNNNNNNNGG 1 cut(s) 80
BsmFI GGGAC 2 cut(s) 111, 378
BsmI GAATGC 1 cut(s) 420
BsnI GGCC 1 cut(s) 566
BsoBI CYCGRG 1 cut(s) 576
Bsp1286I GDGCHC 2 cut(s) 73, 116
Bsp143I GATC 2 cut(s) 319, 462
BspANI GGCC 1 cut(s) 566
BspCNI CTCAG 3 cut(s) 195, 304, 435
BspLI GGNNCC 2 cut(s) 115, 134
BspPI GGATC 1 cut(s) 470
BssECI CCNNGG 3 cut(s) 128, 129, 576
BssMI GATC 2 cut(s) 319, 462
Bst2UI CCWGG 2 cut(s) 130, 596
BstDEI CTNAG 4 cut(s) 182, 291, 330, 443
BstF5I GGATG 3 cut(s) 16, 271, 304
BstKTI GATC 2 cut(s) 322, 465
BstMBI GATC 2 cut(s) 319, 462
BstMWI GCNNNNNNNGC 3 cut(s) 43, 203, 563
BstNI CCWGG 2 cut(s) 130, 596
BstSCI CCNGG 5 cut(s) 73, 128, 575, 576, 594
BstSFI CTRYAG 1 cut(s) 190
BstSLI GKGCMC 1 cut(s) 73
BstV1I GCAGC 1 cut(s) 405
Bsu36I CCTNAGG 1 cut(s) 291
BsuI GTATCC 1 cut(s) 534
BsuRI GGCC 1 cut(s) 566
BtsCI GGATG 3 cut(s) 16, 271, 304
Cfr13I GGNCC 3 cut(s) 133, 287, 532
Cfr9I CCCGGG 1 cut(s) 576
CpoI CGGWCCG 1 cut(s) 532
CsiI ACCWGGT 1 cut(s) 594
Csp6I GTAC 1 cut(s) 507
CspI CGGWCCG 1 cut(s) 532
CviAII CATG 3 cut(s) 7, 109, 368
CviJI RGCY 9 cut(s) 46, 114, 181, 280, 387, 395, 401, 486, 566
CviKI_1 RGCY 9 cut(s) 46, 114, 181, 280, 387, 395, 401, 486, 566
CviQI GTAC 1 cut(s) 507
DdeI CTNAG 4 cut(s) 182, 291, 330, 443
DpnI GATC 2 cut(s) 321, 464
DpnII GATC 2 cut(s) 319, 462
EaeI YGGCCR 1 cut(s) 564
Eco24I GRGCYC 1 cut(s) 116
Eco47I GGWCC 3 cut(s) 133, 287, 532
Eco81I CCTNAGG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 576
EcoRII CCWGG 2 cut(s) 128, 594
EcoT38I GRGCYC 1 cut(s) 116
FaeI CATG 3 cut(s) 10, 112, 371
FaqI GGGAC 2 cut(s) 111, 378
FatI CATG 3 cut(s) 6, 108, 367
Fnu4HI GCNGC 1 cut(s) 419
FokI GGATG 3 cut(s) 23, 278, 311
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 1 cut(s) 419
GluI GCNGC 1 cut(s) 419
GsaI CCCAGC 1 cut(s) 284
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 1 cut(s) 566
HapII CCGG 3 cut(s) 75, 535, 577
Hin1II CATG 3 cut(s) 10, 112, 371
HinfI GANTC 3 cut(s) 439, 494, 527
HpaII CCGG 3 cut(s) 75, 535, 577
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 2 cut(s) 71, 526
Hpy188I TCNGA 1 cut(s) 444
Hpy188III TCNNGA 2 cut(s) 156, 248
Hpy8I GTNNAC 2 cut(s) 71, 526
HpyAV CCTTC 2 cut(s) 209, 443
HpyCH4V TGCA 4 cut(s) 71, 206, 421, 583
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 203, 563
HpyF3I CTNAG 4 cut(s) 182, 291, 330, 443
Hsp92II CATG 3 cut(s) 10, 112, 371
Kzo9I GATC 2 cut(s) 319, 462
LmnI GCTCC 1 cut(s) 119
Lsp1109I GCAGC 1 cut(s) 405
MabI ACCWGGT 1 cut(s) 594
MalI GATC 2 cut(s) 321, 464
MboI GATC 2 cut(s) 319, 462
MboII GAAGA 2 cut(s) 314, 582
MhlI GDGCHC 2 cut(s) 73, 116
MlsI TGGCCA 1 cut(s) 566
MluCI AATT 1 cut(s) 431
MluNI TGGCCA 1 cut(s) 566
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 6 cut(s) 127, 192, 204, 300, 348, 366
Mox20I TGGCCA 1 cut(s) 566
MscI TGGCCA 1 cut(s) 566
MseI TTAA 1 cut(s) 389
Msp20I TGGCCA 1 cut(s) 566
MspI CCGG 3 cut(s) 75, 535, 577
MspR9I CCNGG 5 cut(s) 75, 130, 577, 578, 596
Mva1269I GAATGC 1 cut(s) 420
MvaI CCWGG 2 cut(s) 130, 596
MwoI GCNNNNNNNGC 3 cut(s) 43, 203, 563
NciI CCSGG 3 cut(s) 75, 577, 578
NdeII GATC 2 cut(s) 319, 462
NlaIII CATG 3 cut(s) 10, 112, 371
NlaIV GGNNCC 2 cut(s) 115, 134
PasI CCCWGGG 1 cut(s) 129
PctI GAATGC 1 cut(s) 420
PfeI GAWTC 2 cut(s) 439, 494
PflFI GACNNNGTC 1 cut(s) 530
PkrI GCNGC 1 cut(s) 420
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
Psp6I CCWGG 2 cut(s) 128, 594
PspFI CCCAGC 1 cut(s) 280
PspGI CCWGG 2 cut(s) 128, 594
PspN4I GGNNCC 2 cut(s) 115, 134
PspPI GGNCC 3 cut(s) 133, 287, 532
PsyI GACNNNGTC 1 cut(s) 530
RsaI GTAC 1 cut(s) 508
RsaNI GTAC 1 cut(s) 507
Rsr2I CGGWCCG 1 cut(s) 532
RsrII CGGWCCG 1 cut(s) 532
SaqAI TTAA 1 cut(s) 389
SatI GCNGC 1 cut(s) 419
Sau3AI GATC 2 cut(s) 319, 462
Sau96I GGNCC 3 cut(s) 133, 287, 532
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 5 cut(s) 75, 130, 577, 578, 596
SduI GDGCHC 2 cut(s) 73, 116
SetI ASST 7 cut(s) 183, 282, 297, 336, 397, 488, 597
SexAI ACCWGGT 1 cut(s) 594
SfcI CTRYAG 1 cut(s) 190
SinI GGWCC 3 cut(s) 133, 287, 532
SmaI CCCGGG 1 cut(s) 578
Sse9I AATT 1 cut(s) 431
StyD4I CCNGG 5 cut(s) 73, 128, 575, 576, 594
TaqII GACCGA 1 cut(s) 520
TasI AATT 1 cut(s) 431
TfiI GAWTC 2 cut(s) 439, 494
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TseI GCWGC 1 cut(s) 418
TspDTI ATGAA 1 cut(s) 33
TspGWI ACGGA 1 cut(s) 125
TspMI CCCGGG 1 cut(s) 576
Tth111I GACNNNGTC 1 cut(s) 530
VneI GTGCAC 1 cut(s) 69
VpaK11BI GGWCC 3 cut(s) 133, 287, 532
XmaI CCCGGG 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.