Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002957

Physical Location & Seq

Genomic Coordinates Forward (+)

8226653 .. 8233660

Gene Structure 7008 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1048400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1974 bp

ATGGGAAGCACCTTTCTCTCTCTACCAGCGAAACGTTCCCTATCGACTATGTCTTCTGACCCCAACCCCTACTTCGAAAACGGATCCTCCAAGCGCTCCAGGCCCCTTCCTCCTCCCCTCAGCGTTCCTCCCGCCCACGTCGCCTTCCGCATGCTCTGCCATGCCTCGCGCATCGGCGGCGTTATCGGAAAGTCAGGTGCCGTGATCAAGCAGCTCCAGAAAACCACCGGAGCCAAGATCCGAATTGAAGAACCCCAAGTCGAATCCCCCGACCGGGTCGTTGTGGTCATCGCTCCGAGCACAATCCGGTCAAAGATTTGGCTCAAAACTCCAGCTCTCGGCAATGTTAATAATGGTGGCGGTGGTGGTGTCGAAGAGGAGATTGATGTTTCCCAGGCTCAGGAGGCGCTTCTGAGAGTGTTCGAGAGGATTCTGGAAGTTGCGGCGGAGACCGGGGCGATTGCGGCGGGGGTTGGGGTGGTGTCGTGCAGGTTTTTGGCGGAGGCGGCCCAGGTCGGGTCAGTGATAGGAAAGGGGGGGAAGGTGGTAGAGAAGATTAGGAAAGAAACTGGTTGTAAAATTAGGGTTTTGAATGAGAAGTTACCTGCTTGTGCTGCAGCCACAGACGAGATGATCGAGATAGAAGGGGATATTTTGGCAGTAAAGAAAGCACTTGTTGCTGTCTCTGGCTGTCTCCAAGATTGTCCACCAATTGACAAGACAAGGATGACTGGAAGCAGGCCTGAGGCAGTTCCTCGTGAAACTCTACCTGATCTGCGTTTAGATCAACTTTCACAGAGGAACTCAATGCTTAATTTGCCAATCAGCTCCATGAGTTATGCTACAGGAGTTCGTCCATCCTCAATAGAGGCTGAAAGGATCCCCACACTTGAGACAAAACAACAGGAAGTTTCTTTCAGGATTCTTTGTGCCAATGATAGGATTGGGGGTGTAATTGGAAGGGGGGGTTCCATTGTCAGGGCTCTTCAAAATGAGACAGGTGCTTCAATAAGTATTGCAGCATCTGTGGCTGAATGTGATGAACGATTAATTACTGTTTCTGCATCGGAGAATCCTGAATCAAGGTACTCGCCAACACAAAAGGCTGCTGTTCTTGTCTTCTCAAGGTCCGTAGAGGCAGGCATAGAAAAGGGGCGAGACTCAAGCTCAAATAAAGGTTCACCCCTTACTGCACGGCTTGTAATTCCATCAAGCCAAGTTGGGTGTTTGCTGGGAAAAGGGGGTGCAATAGTTTCAGAGATCCGGAAGCAAACTGGTACTGGTATACGAATAATAGGGGGTGACCAGGTCCCGAAATGTGCCTTAGAGAATGATGAAGTTGTACAGATTTCAGGAGATTTTTCAAACGTGCAAGATGCTTTGTACAATATTACTGGTAGATTACGAGATAACCTTTTCTCCAGCACAGTAAGTAATTCTGGAAGAAGGAGCAGTTCATCCATGCTAACGGCAACCGATACAAGTCCTTATGGAAGACCTAGGGATCCAGCTCTTATTGGATTTCAGCCATCGTCATCAGTTGGTGTTACTCATGGTCTCAGCCGTCATACAACACTAATTCAAAGTATGGATCATCTTGGTCTTTCCCATAGTTTAGATCATCCTTCCCCACCAAGACCATGGGCATCACAGTTGGCGGCTTTCACAGATGTTGGAAGGGGTTTGACTTCTCTAAAAAGTCGCACAGAAATTGGCAGCGGGAGCAAATCTGCTATTGTGACAAATACAACCATAGAGATCGTAGTTCCTGAAAATGTTATTGGCTCTGTGTATGGGGAGAATGGTACCAATTTGGCTCGTCTGAGACAGATTTCTGGTGCCAAGGTCATAGTGCATGAGCCCCGTCCTGGAACAACTGATAGGATTATTGTCATATCTGGGACACCAGATGAAACCCAGGCAGCACAAAGCCTTCTACATGCATTCATTCTTACTGGACCGTCACACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0007275 GO:0008150 GO:0009791 GO:0009908 GO:0009909 GO:0009911 GO:0016604 GO:0016607 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045596 GO:0048367 GO:0048437 GO:0048497 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051239 GO:0051240 GO:0061458 GO:0065007 GO:0070013 GO:0090567 GO:0090700 GO:0099402 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

658

Amino Acids

69.44

Weight (kDa)

8.99

Isoelectric Point (pI)

50.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_1	PF00013	49 - 98	1.6e-12	KH domain
KH_1	PF00013	163 - 228	1.5e-13	KH domain
KH_1	PF00013	306 - 357	1.4e-11	KH domain
KH_1	PF00013	397 - 464	4.2e-16	KH domain
KH_1	PF00013	584 - 647	1.1e-13	KH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002957)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64390 AT5G64390 AT5G64390
fragaria_vesca	FvH4_2g11560 FvH4_2g11560 FvH4_2g11560
malus_domestica	MD05G1048400.v1.1 MD10G1054800.v1.1
prunus_persica	Prupe.8G070900_v2.0.a1
pyrus_communis	pycom05g04000 pycom10g04180
rosa_chinensis	RchiOBHm_Chr1g0353311 RchiOBHm_Chr4g0385021 RchiOBHm_Chr6g0270731 RchiOBHm_Chr6g0272811
rosa_laevigata	RLG00000013774
rosa_multiflora	Rmu_co8284529.1_g000001 Rmu_sc0000642.1_g000012 Rmu_sc0041041.1_g000001
rosa_roxburghii	Rroxscaffold_2G00085770 Rroxscaffold_7G00197640
rosa_rugosa	Rorug06G0056000 Rorug06G0056100 Rorug06G0056200
rosa_samantha	Rh2DG204000 Rh4CG002400 Rh4CG168900 Rh6AG157400 Rh6AG175200 Rh6BG140200 Rh6BG178600 Rh6CG174800 Rh6DG123600 Rh6DG167400 Rh6DG180500 Rh7AG209600 Rh7CG359700
rosa_wichuraiana	Rw6G014930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	480, 613
Acc65I	GGTACC	1 cut(s)	1805
AccB1I	GGYRCC	3 cut(s)	197, 1805, 1838
AccI	GTMKAC	1 cut(s)	1285
AccII	CGCG	1 cut(s)	169
AccIII	TCCGGA	1 cut(s)	1263
AclI	AACGTT	1 cut(s)	34
AclWI	GGATC	9 cut(s)	78, 91, 232, 874, 887, 1255, 1499, 1512, 1599
AfaI	GTAC	5 cut(s)	1088, 1279, 1344, 1385, 1807
AfeI	AGCGCT	1 cut(s)	95
AfiI	CCNNNNNNNGG	8 cut(s)	273, 274, 338, 400, 516, 939, 978, 1868
AgsI	TTSAA	6 cut(s)	248, 592, 989, 1008, 1365, 1583
AjiI	CACGTC	1 cut(s)	139
AjnI	CCWGG	6 cut(s)	98, 393, 510, 1305, 1867, 1917
AjuI	GAANNNNNNNTTGG	4 cut(s)	56, 88, 1764, 1796
AluBI	AGCT	5 cut(s)	214, 335, 828, 1167, 1511
AluI	AGCT	5 cut(s)	214, 335, 828, 1167, 1511
Alw21I	GWGCWC	1 cut(s)	302
Alw26I	GTCTC	8 cut(s)	443, 688, 698, 887, 989, 1152, 1562, 1819
AlwI	GGATC	9 cut(s)	78, 91, 232, 874, 887, 1255, 1499, 1512, 1599
AlwNI	CAGNNNCTG	1 cut(s)	1025
Aor13HI	TCCGGA	1 cut(s)	1263
Aor51HI	AGCGCT	1 cut(s)	95
AoxI	GGCC	3 cut(s)	101, 507, 740
ApeKI	GCWGC	7 cut(s)	211, 614, 617, 1019, 1106, 1716, 1922
AseI	ATTAAT	1 cut(s)	1049
Asp718I	GGTACC	1 cut(s)	1805
AspA2I	CCTAGG	1 cut(s)	1499
AspLEI	GCGC	3 cut(s)	96, 171, 409
AspS9I	GGNCC	5 cut(s)	102, 508, 1128, 1309, 1958
AsuC2I	CCSGG	2 cut(s)	275, 454
AsuHPI	GGTGA	2 cut(s)	1173, 1313
AsuII	TTCGAA	1 cut(s)	75
AvaII	GGWCC	3 cut(s)	1128, 1309, 1958
AvrII	CCTAGG	1 cut(s)	1499
AxyI	CCTNAGG	1 cut(s)	744
BamHI	GGATCC	3 cut(s)	83, 879, 1504
BanI	GGYRCC	3 cut(s)	197, 1805, 1838
BanII	GRGCYC	2 cut(s)	985, 1863
BauI	CACGAG	1 cut(s)	756
BbsI	GAAGAC	3 cut(s)	45, 1111, 1501
Bbv12I	GWGCWC	1 cut(s)	302
BbvCI	CCTCAGC	1 cut(s)	119
BbvI	GCAGC	7 cut(s)	223, 601, 629, 1031, 1093, 1728, 1934
BccI	CCATC	3 cut(s)	865, 1216, 1537
BceAI	ACGGC	4 cut(s)	185, 1211, 1485, 1548
BciT130I	CCWGG	6 cut(s)	100, 395, 512, 1307, 1869, 1919
BclI	TGATCA	1 cut(s)	204
BcnI	CCSGG	2 cut(s)	275, 454
BcoDI	GTCTC	8 cut(s)	443, 688, 698, 887, 989, 1152, 1562, 1819
BfaI	CTAG	1 cut(s)	1500
BfmI	CTRYAG	2 cut(s)	615, 843
BfoI	RGCGCY	2 cut(s)	97, 410
BfuAI	ACCTGC	2 cut(s)	480, 613
BlnI	CCTAGG	1 cut(s)	1499
Bme1390I	CCNGG	8 cut(s)	100, 275, 395, 454, 512, 1307, 1869, 1919
Bme18I	GGWCC	3 cut(s)	1128, 1309, 1958
BmgBI	CACGTC	1 cut(s)	139
BmgT120I	GGNCC	5 cut(s)	102, 508, 1128, 1309, 1958
BmrFI	CCNGG	8 cut(s)	100, 275, 395, 454, 512, 1307, 1869, 1919
BmsI	GCATC	5 cut(s)	180, 1031, 1073, 1366, 1655
BpiI	GAAGAC	3 cut(s)	45, 1111, 1501
BpmI	CTGGAG	4 cut(s)	82, 200, 315, 1405
Bpu10I	CCTNAGC	2 cut(s)	119, 399
Bpu14I	TTCGAA	1 cut(s)	75
BpuEI	CTTGAG	3 cut(s)	911, 1108, 1147
BpuMI	CCSGG	2 cut(s)	275, 454
BsaI	GGTCTC	2 cut(s)	443, 1562
BsaJI	CCNNGG	7 cut(s)	393, 453, 510, 1499, 1640, 1842, 1917
BsaWI	WCCGGW	3 cut(s)	227, 306, 1263
BsaXI	ACNNNNNCTCC	4 cut(s)	71, 101, 1403, 1433
Bsc4I	CCNNNNNNNGG	8 cut(s)	273, 274, 338, 400, 516, 939, 978, 1868
Bse1I	ACTGG	6 cut(s)	574, 736, 1279, 1285, 1399, 1960
Bse21I	CCTNAGG	1 cut(s)	744
Bse3DI	GCAATG	1 cut(s)	349
BseAI	TCCGGA	1 cut(s)	1263
BseBI	CCWGG	6 cut(s)	100, 395, 512, 1307, 1869, 1919
BseDI	CCNNGG	7 cut(s)	393, 453, 510, 1499, 1640, 1842, 1917
BseGI	GGATG	4 cut(s)	732, 857, 1457, 1621
BseLI	CCNNNNNNNGG	8 cut(s)	273, 274, 338, 400, 516, 939, 978, 1868
BseMI	GCAATG	1 cut(s)	349
BseMII	CTCAG	6 cut(s)	133, 404, 413, 735, 1573, 1814
BseNI	ACTGG	6 cut(s)	574, 736, 1279, 1285, 1399, 1960
BseRI	GAGGAG	2 cut(s)	102, 392
BseXI	GCAGC	7 cut(s)	223, 601, 629, 1031, 1093, 1728, 1934
BseYI	CCCAGC	1 cut(s)	1231
BsgI	GTGCAG	2 cut(s)	508, 1176
Bsh1236I	CGCG	1 cut(s)	169
Bsh1285I	CGRYCG	1 cut(s)	274
BshFI	GGCC	3 cut(s)	103, 509, 742
BshNI	GGYRCC	3 cut(s)	197, 1805, 1838
BsiEI	CGRYCG	1 cut(s)	274
BsiHKAI	GWGCWC	1 cut(s)	302
BsiSI	CCGG	5 cut(s)	228, 274, 307, 453, 1264
BslFI	GGGAC	2 cut(s)	1295, 1915
BslI	CCNNNNNNNGG	8 cut(s)	273, 274, 338, 400, 516, 939, 978, 1868
BsmAI	GTCTC	8 cut(s)	443, 688, 698, 887, 989, 1152, 1562, 1819
BsmFI	GGGAC	2 cut(s)	1295, 1915
BsmI	GAATGC	1 cut(s)	1943
BsnI	GGCC	3 cut(s)	103, 509, 742
Bso31I	GGTCTC	2 cut(s)	443, 1562
Bsp119I	TTCGAA	1 cut(s)	75
Bsp1286I	GDGCHC	3 cut(s)	302, 985, 1863
Bsp13I	TCCGGA	1 cut(s)	1263
Bsp1407I	TGTACA	2 cut(s)	1342, 1383
Bsp19I	CCATGG	1 cut(s)	1640
BspANI	GGCC	3 cut(s)	103, 509, 742
BspCNI	CTCAG	6 cut(s)	132, 405, 412, 736, 1572, 1815
BspEI	TCCGGA	1 cut(s)	1263
BspFNI	CGCG	1 cut(s)	169
BspMAI	CTGCAG	1 cut(s)	619
BspMI	ACCTGC	2 cut(s)	480, 613
BspPI	GGATC	9 cut(s)	78, 91, 232, 874, 887, 1255, 1499, 1512, 1599
BspQI	GCTCTTC	1 cut(s)	990
BspT104I	TTCGAA	1 cut(s)	75
BspT107I	GGYRCC	3 cut(s)	197, 1805, 1838
BspTNI	GGTCTC	2 cut(s)	443, 1562
BsrDI	GCAATG	1 cut(s)	349
BsrGI	TGTACA	2 cut(s)	1342, 1383
BsrI	ACTGG	6 cut(s)	574, 736, 1279, 1285, 1399, 1960
BssECI	CCNNGG	7 cut(s)	393, 453, 510, 1499, 1640, 1842, 1917
BssNAI	GTATAC	1 cut(s)	1286
BssSI	CACGAG	1 cut(s)	756
BssT1I	CCWWGG	3 cut(s)	1499, 1640, 1842
Bst1107I	GTATAC	1 cut(s)	1286
Bst2BI	CACGAG	1 cut(s)	756
Bst2UI	CCWGG	6 cut(s)	100, 395, 512, 1307, 1869, 1919
Bst4CI	ACNGT	4 cut(s)	1057, 1429, 1653, 1962
Bst6I	CTCTTC	2 cut(s)	369, 990
BstAPI	GCANNNNNTGC	2 cut(s)	156, 677
BstAUI	TGTACA	2 cut(s)	1342, 1383
BstBI	TTCGAA	1 cut(s)	75
BstC8I	GCNNGC	3 cut(s)	152, 740, 1141
BstDEI	CTNAG	7 cut(s)	119, 399, 413, 744, 1324, 1559, 1823
BstDSI	CCRYGG	1 cut(s)	1640
BstEII	GGTNACC	1 cut(s)	1301
BstF5I	GGATG	4 cut(s)	732, 857, 1457, 1621
BstFNI	CGCG	1 cut(s)	169
BstH2I	RGCGCY	2 cut(s)	97, 410
BstHHI	GCGC	3 cut(s)	96, 171, 409
BstMAI	GTCTC	8 cut(s)	443, 688, 698, 887, 989, 1152, 1562, 1819
BstMCI	CGRYCG	1 cut(s)	274
BstNI	CCWGG	6 cut(s)	100, 395, 512, 1307, 1869, 1919
BstNSI	RCATGY	2 cut(s)	154, 1943
BstPI	GGTNACC	1 cut(s)	1301
BstSCI	CCNGG	8 cut(s)	98, 273, 393, 452, 510, 1305, 1867, 1917
BstSFI	CTRYAG	2 cut(s)	615, 843
BstUI	CGCG	1 cut(s)	169
BstV1I	GCAGC	7 cut(s)	223, 601, 629, 1031, 1093, 1728, 1934
BstV2I	GAAGAC	3 cut(s)	45, 1111, 1501
BstX2I	RGATCY	5 cut(s)	83, 237, 879, 1260, 1504
BstXI	CCANNNNNNTGG	1 cut(s)	1641
BstYI	RGATCY	5 cut(s)	83, 237, 879, 1260, 1504
BstZ17I	GTATAC	1 cut(s)	1286
Bsu36I	CCTNAGG	1 cut(s)	744
BsuRI	GGCC	3 cut(s)	103, 509, 742
BtgI	CCRYGG	1 cut(s)	1640
BtgZI	GCGATG	1 cut(s)	274
BtrI	CACGTC	1 cut(s)	139
BtsCI	GGATG	4 cut(s)	732, 857, 1457, 1621
BtsIMutI	CAGTG	1 cut(s)	528
BveI	ACCTGC	2 cut(s)	480, 613
Cac8I	GCNNGC	3 cut(s)	152, 740, 1141
CaiI	CAGNNNCTG	1 cut(s)	1025
CfoI	GCGC	3 cut(s)	96, 171, 409
Cfr13I	GGNCC	5 cut(s)	102, 508, 1128, 1309, 1958
CsiI	ACCWGGT	1 cut(s)	1305
Csp6I	GTAC	5 cut(s)	1087, 1278, 1343, 1384, 1806
CviAII	CATG	8 cut(s)	151, 161, 832, 1462, 1553, 1641, 1856, 1940
CviQI	GTAC	5 cut(s)	1087, 1278, 1343, 1384, 1806
DdeI	CTNAG	7 cut(s)	119, 399, 413, 744, 1324, 1559, 1823
Eam1104I	CTCTTC	2 cut(s)	369, 990
EarI	CTCTTC	2 cut(s)	369, 990
EciI	GGCGGA	2 cut(s)	461, 515
Eco130I	CCWWGG	3 cut(s)	1499, 1640, 1842
Eco147I	AGGCCT	1 cut(s)	742
Eco24I	GRGCYC	2 cut(s)	985, 1863
Eco31I	GGTCTC	2 cut(s)	443, 1562
Eco47I	GGWCC	3 cut(s)	1128, 1309, 1958
Eco47III	AGCGCT	1 cut(s)	95
Eco81I	CCTNAGG	1 cut(s)	744
Eco91I	GGTNACC	1 cut(s)	1301
EcoO109I	RGGNCCY	2 cut(s)	102, 1309
EcoO65I	GGTNACC	1 cut(s)	1301
EcoRII	CCWGG	6 cut(s)	98, 393, 510, 1305, 1867, 1917
EcoT14I	CCWWGG	3 cut(s)	1499, 1640, 1842
EcoT22I	ATGCAT	1 cut(s)	1945
EcoT38I	GRGCYC	2 cut(s)	985, 1863
ErhI	CCWWGG	3 cut(s)	1499, 1640, 1842
FaeI	CATG	8 cut(s)	154, 164, 835, 1465, 1556, 1644, 1859, 1943
FaqI	GGGAC	2 cut(s)	1295, 1915
FatI	CATG	8 cut(s)	150, 160, 831, 1461, 1552, 1640, 1855, 1939
FauI	CCCGC	3 cut(s)	139, 460, 1712
FbaI	TGATCA	1 cut(s)	204
FblI	GTMKAC	1 cut(s)	1285
FokI	GGATG	4 cut(s)	739, 844, 1444, 1608
FriOI	GRGCYC	2 cut(s)	985, 1863
FspBI	CTAG	1 cut(s)	1500
GlaI	GCGC	3 cut(s)	95, 170, 408
GsaI	CCCAGC	1 cut(s)	1235
GsuI	CTGGAG	4 cut(s)	82, 200, 315, 1405
HaeII	RGCGCY	2 cut(s)	97, 410
HaeIII	GGCC	3 cut(s)	103, 509, 742
HapII	CCGG	5 cut(s)	228, 274, 307, 453, 1264
HhaI	GCGC	3 cut(s)	96, 171, 409
Hin1II	CATG	8 cut(s)	154, 164, 835, 1465, 1556, 1644, 1859, 1943
Hin6I	GCGC	3 cut(s)	94, 169, 407
HinP1I	GCGC	3 cut(s)	94, 169, 407
HinfI	GANTC	6 cut(s)	263, 430, 922, 1072, 1079, 1160
HpaII	CCGG	5 cut(s)	228, 274, 307, 453, 1264
HphI	GGTGA	2 cut(s)	1173, 1313
Hpy166II	GTNNAC	3 cut(s)	707, 1181, 1286
Hpy188I	TCNGA	8 cut(s)	58, 188, 242, 297, 414, 1069, 1258, 1824
Hpy8I	GTNNAC	3 cut(s)	707, 1181, 1286
Hpy99I	CGWCG	1 cut(s)	143
HpyAV	CCTTC	9 cut(s)	116, 154, 535, 638, 954, 1440, 1635, 1671, 1943
HpyCH4III	ACNGT	4 cut(s)	1057, 1429, 1653, 1962
HpyCH4IV	ACGT	3 cut(s)	34, 138, 1368
HpyCH4V	TGCA	9 cut(s)	489, 617, 1019, 1064, 1193, 1247, 1372, 1855, 1943
HpyF3I	CTNAG	7 cut(s)	119, 399, 413, 744, 1324, 1559, 1823
HpySE526I	ACGT	3 cut(s)	34, 138, 1368
Hsp92II	CATG	8 cut(s)	154, 164, 835, 1465, 1556, 1644, 1859, 1943
HspAI	GCGC	3 cut(s)	94, 169, 407
Kpn2I	TCCGGA	1 cut(s)	1263
KpnI	GGTACC	1 cut(s)	1809
Ksp22I	TGATCA	1 cut(s)	204
LguI	GCTCTTC	1 cut(s)	990
LmnI	GCTCC	7 cut(s)	101, 219, 230, 298, 833, 1449, 1722
Lsp1109I	GCAGC	7 cut(s)	223, 601, 629, 1031, 1093, 1728, 1934
LweI	GCATC	5 cut(s)	180, 1031, 1073, 1366, 1655
MabI	ACCWGGT	1 cut(s)	1305
MaeI	CTAG	1 cut(s)	1500
MaeII	ACGT	3 cut(s)	34, 138, 1368
MaeIII	GTNAC	5 cut(s)	600, 1301, 1546, 1738, 1962
MboII	GAAGA	8 cut(s)	45, 260, 386, 565, 977, 1111, 1455, 1506
MfeI	CAATTG	1 cut(s)	711
MflI	RGATCY	5 cut(s)	83, 237, 879, 1260, 1504
MhlI	GDGCHC	3 cut(s)	302, 985, 1863
MlyI	GAGTC	1 cut(s)	1154
MmeI	TCCRAC	1 cut(s)	1654
Mph1103I	ATGCAT	1 cut(s)	1945
MroI	TCCGGA	1 cut(s)	1263
MseI	TTAA	3 cut(s)	348, 813, 1049
MspA1I	CMGCKG	1 cut(s)	1719
MspI	CCGG	5 cut(s)	228, 274, 307, 453, 1264
MspR9I	CCNGG	8 cut(s)	100, 275, 395, 454, 512, 1307, 1869, 1919
MunI	CAATTG	1 cut(s)	711
Mva1269I	GAATGC	1 cut(s)	1943
MvaI	CCWGG	6 cut(s)	100, 395, 512, 1307, 1869, 1919
MvnI	CGCG	1 cut(s)	169
NciI	CCSGG	2 cut(s)	275, 454
NcoI	CCATGG	1 cut(s)	1640
NlaIII	CATG	8 cut(s)	154, 164, 835, 1465, 1556, 1644, 1859, 1943
NmeAIII	GCCGAG	1 cut(s)	318
NmuCI	GTSAC	3 cut(s)	1301, 1738, 1962
NsiI	ATGCAT	1 cut(s)	1945
NspI	RCATGY	2 cut(s)	154, 1943
NspV	TTCGAA	1 cut(s)	75
PaeI	GCATGC	1 cut(s)	154
PceI	AGGCCT	1 cut(s)	742
PciSI	GCTCTTC	1 cut(s)	990
PcsI	WCGNNNNNNNCGW	2 cut(s)	267, 633
PctI	GAATGC	1 cut(s)	1943
PfeI	GAWTC	5 cut(s)	263, 430, 922, 1072, 1079
PflFI	GACNNNGTC	3 cut(s)	49, 275, 1307
PfoI	TCCNGGA	1 cut(s)	1867
PleI	GAGTC	1 cut(s)	1154
PpsI	GAGTC	1 cut(s)	1154
PpuMI	RGGWCCY	1 cut(s)	1309
PshBI	ATTAAT	1 cut(s)	1049
Psp1406I	AACGTT	1 cut(s)	34
Psp5II	RGGWCCY	1 cut(s)	1309
Psp6I	CCWGG	6 cut(s)	98, 393, 510, 1305, 1867, 1917
PspEI	GGTNACC	1 cut(s)	1301
PspFI	CCCAGC	1 cut(s)	1231
PspGI	CCWGG	6 cut(s)	98, 393, 510, 1305, 1867, 1917
PspPI	GGNCC	5 cut(s)	102, 508, 1128, 1309, 1958
PspPPI	RGGWCCY	1 cut(s)	1309
PstI	CTGCAG	1 cut(s)	619
PstNI	CAGNNNCTG	1 cut(s)	1025
PsuI	RGATCY	5 cut(s)	83, 237, 879, 1260, 1504
PsyI	GACNNNGTC	3 cut(s)	49, 275, 1307
RsaI	GTAC	5 cut(s)	1088, 1279, 1344, 1385, 1807
RsaNI	GTAC	5 cut(s)	1087, 1278, 1343, 1384, 1806
SapI	GCTCTTC	1 cut(s)	990
SaqAI	TTAA	3 cut(s)	348, 813, 1049
Sau96I	GGNCC	5 cut(s)	102, 508, 1128, 1309, 1958
SchI	GAGTC	1 cut(s)	1154
ScrFI	CCNGG	8 cut(s)	100, 275, 395, 454, 512, 1307, 1869, 1919
SduI	GDGCHC	3 cut(s)	302, 985, 1863
SexAI	ACCWGGT	1 cut(s)	1305
SfaNI	GCATC	5 cut(s)	180, 1031, 1073, 1366, 1655
SfcI	CTRYAG	2 cut(s)	615, 843
SfuI	TTCGAA	1 cut(s)	75
SinI	GGWCC	3 cut(s)	1128, 1309, 1958
SmlI	CTYRAG	3 cut(s)	890, 1123, 1162
SmoI	CTYRAG	3 cut(s)	890, 1123, 1162
SphI	GCATGC	1 cut(s)	154
SseBI	AGGCCT	1 cut(s)	742
SspI	AATATT	1 cut(s)	1390
SspMI	CTAG	1 cut(s)	1500
StuI	AGGCCT	1 cut(s)	742
StyD4I	CCNGG	8 cut(s)	98, 273, 393, 452, 510, 1305, 1867, 1917
StyI	CCWWGG	3 cut(s)	1499, 1640, 1842
TaaI	ACNGT	4 cut(s)	1057, 1429, 1653, 1962
TaiI	ACGT	3 cut(s)	37, 141, 1371
TaqI	TCGA	6 cut(s)	44, 75, 261, 372, 423, 636
TatI	WGTACW	2 cut(s)	1342, 1383
TauI	GCSGC	5 cut(s)	180, 446, 467, 509, 1661
TfiI	GAWTC	5 cut(s)	263, 430, 922, 1072, 1079
Tru1I	TTAA	3 cut(s)	348, 813, 1049
Tru9I	TTAA	3 cut(s)	348, 813, 1049
TscAI	CASTG	1 cut(s)	528
TseFI	GTSAC	3 cut(s)	1301, 1738, 1962
TseI	GCWGC	7 cut(s)	211, 614, 617, 1019, 1106, 1716, 1922
Tsp45I	GTSAC	3 cut(s)	1301, 1738, 1962
TspDTI	ATGAA	5 cut(s)	1056, 1350, 1446, 1926, 1936
TspGWI	ACGGA	2 cut(s)	96, 1120
TspRI	CASTG	1 cut(s)	528
Tth111I	GACNNNGTC	3 cut(s)	49, 275, 1307
VpaK11BI	GGWCC	3 cut(s)	1128, 1309, 1958
VspI	ATTAAT	1 cut(s)	1049
XceI	RCATGY	2 cut(s)	154, 1943
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1638
XmaJI	CCTAGG	1 cut(s)	1499
XmiI	GTMKAC	1 cut(s)	1285
XspI	CTAG	1 cut(s)	1500
Zsp2I	ATGCAT	1 cut(s)	1945

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.