RchiOBHm_Chr6g0272811

KH domain-containing protein At4g18375-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002957
Physical Location & Seq
Reverse (-)
33251780 .. 33253291
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24476

Sequence Viewer

Length: 1140 bp
ATGATTCTTTGCTTCCATTGGGGTATAGTTCAGTTATTCTGTTTTTTTTCTTCCCTTGAAAATGATTCAATGATGTGGCAGATAGAAGGGGATATTTTGGCTGTAAAGAAAGCACTTGTTGCTGTCTCCGGCCATCTTCAAGATTGTCCACCAGTTGACAAGACAAGGATGACTGGAAGTAGGCCTATTGAGACAGTTCCTCGTGAAACTTTACCTGATATTCGTGTAGATCACCTTTCACAGAGGAACTCAGTGATTTCCAATTTGCCGAGCAGCGCCATGAGTTATGCTTCAGGAGTTTGTCTGCCATCAATAGAGGCTGAAAGGATCTCAACACTGGATACAAGAACACTCCAGCAGGAAGTTACTTTTAAGATTCTTTGCGCCAATGATAGGGTTGGGGGTGTCATTGGAAAAGGGGGTGCCATTGTCAAGGCTCTTCAGAATGAGACTGGTGCTACTATAAGTGTTGGAGGATCTGTGGCCGAGTGTGATGAAAGATTAATTACTAATCCTGAGTCCCGCTACTCTCCAGCACAAAAAGGTCTTGTTCTTGTCTTCTCAAGGTCTGTAGAGGCAGGCATTGAAAAGAGGCAAGACTCGAGCTCCAAAGGGTCACCTCTTATTGCACGCTTTGTTCTCCCGACAAGCCAAATTCGGAAGGTAACTGGTACCGGTATACGGATAATAGGTGGTGTCCAGGTCCCGAAGTGTGCCGTAGAGAATGACGAAGTTGTACAGGTGTCAAGAAATTTTTCAAGTGTGCAAGATGCTTTGTACAATATTACTGGTAGGTTACGAGATAACCTTTTCTCCAACACAGTAAATAATTCTGGAAGGAGGAATAGTTCAGCCGTGCTAACAGATACCAATCCTTATGGAAGACTCAGGGATCCTGGTCCTACTGGATTTCAGCCTTCTTCATCAGTTGGTGTTACTCATGGTCTCGGTCGACATACTACTCTAACTCAAAGTATGGATCATCTTGGACTTTCCCATAATTTAGATCATCCTTCTTCACCAAGGCCATGGGCATCACAGATGGTTGCTCCAAGGGGAATCTCAGATACTAGCAGGGGTTTGACTTCTCTTAAAAGTGGCTCGGATCTTGGTAGGTTAGTAATGTCGCACAATGCATAG

Protein Analysis

379

Amino Acids

40.85

Weight (kDa)

8.86

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_1 PF00013 123 - 170 6.6e-10 KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 671
AccB1I GGYRCC 2 cut(s) 422, 671
AccI GTMKAC 2 cut(s) 679, 952
AciI CCGC 1 cut(s) 523
AclWI GGATC 6 cut(s) 335, 484, 887, 900, 987, 1113
AcoI YGGCCR 2 cut(s) 130, 483
AcsI RAATTY 2 cut(s) 654, 751
AcuI CTGAAG 2 cut(s) 276, 425
AfaI GTAC 3 cut(s) 673, 738, 779
AfiI CCNNNNNNNGG 2 cut(s) 393, 681
AgeI ACCGGT 1 cut(s) 674
AgsI TTSAA 5 cut(s) 59, 69, 140, 587, 759
AjnI CCWGG 2 cut(s) 699, 895
AloI GAACNNNNNNTCC 2 cut(s) 832, 864
AluBI AGCT 1 cut(s) 606
AluI AGCT 1 cut(s) 606
Alw21I GWGCWC 1 cut(s) 608
Alw26I GTCTC 4 cut(s) 130, 185, 443, 950
AlwI GGATC 6 cut(s) 335, 484, 887, 900, 987, 1113
Ama87I CYCGRG 1 cut(s) 601
AoxI GGCC 4 cut(s) 130, 182, 483, 1025
ApeKI GCWGC 1 cut(s) 273
ApoI RAATTY 2 cut(s) 654, 751
AseI ATTAAT 1 cut(s) 503
AsiGI ACCGGT 1 cut(s) 674
Asp700I GAANNNNTTC 1 cut(s) 754
Asp718I GGTACC 1 cut(s) 671
AspLEI GCGC 2 cut(s) 278, 386
AspS9I GGNCC 2 cut(s) 703, 899
AsuHPI GGTGA 3 cut(s) 224, 609, 1011
AvaI CYCGRG 1 cut(s) 601
AvaII GGWCC 2 cut(s) 703, 899
BamHI GGATCC 1 cut(s) 892
BanI GGYRCC 2 cut(s) 422, 671
BanII GRGCYC 1 cut(s) 608
BauI CACGAG 1 cut(s) 201
BbsI GAAGAC 2 cut(s) 550, 889
Bbv12I GWGCWC 1 cut(s) 608
BbvI GCAGC 1 cut(s) 285
BccI CCATC 3 cut(s) 141, 316, 1036
BceAI ACGGC 2 cut(s) 701, 839
BciT130I CCWGG 2 cut(s) 701, 897
BciVI GTATCC 1 cut(s) 334
BcoDI GTCTC 4 cut(s) 130, 185, 443, 950
BfaI CTAG 1 cut(s) 1071
BfmI CTRYAG 1 cut(s) 570
BfoI RGCGCY 1 cut(s) 279
BfuI GTATCC 1 cut(s) 334
BisI GCNGC 1 cut(s) 274
BlsI GCNGC 1 cut(s) 275
Bme1390I CCNGG 2 cut(s) 701, 897
Bme18I GGWCC 2 cut(s) 703, 899
BmeT110I CYCGRG 1 cut(s) 601
BmgT120I GGNCC 2 cut(s) 703, 899
BmiI GGNNCC 4 cut(s) 424, 673, 705, 894
BmrFI CCNGG 2 cut(s) 701, 897
BmsI GCATC 2 cut(s) 760, 1043
BpiI GAAGAC 2 cut(s) 550, 889
BpmI CTGGAG 2 cut(s) 338, 516
BpuEI CTTGAG 1 cut(s) 547
BsaBI GATNNNNATC 1 cut(s) 870
BsaI GGTCTC 1 cut(s) 950
BsaJI CCNNGG 3 cut(s) 1022, 1028, 1052
BsaWI WCCGGW 1 cut(s) 674
BsaXI ACNNNNNCTCC 8 cut(s) 465, 495, 590, 620, 797, 827, 832, 862
Bsc4I CCNNNNNNNGG 2 cut(s) 393, 681
Bse118I RCCGGY 1 cut(s) 674
Bse1I ACTGG 7 cut(s) 152, 178, 342, 457, 673, 793, 910
Bse8I GATNNNNATC 1 cut(s) 870
BseBI CCWGG 2 cut(s) 701, 897
BseDI CCNNGG 3 cut(s) 1022, 1028, 1052
BseGI GGATG 2 cut(s) 174, 1009
BseJI GATNNNNATC 1 cut(s) 870
BseLI CCNNNNNNNGG 2 cut(s) 393, 681
BseMII CTCAG 4 cut(s) 264, 507, 901, 1077
BseNI ACTGG 7 cut(s) 152, 178, 342, 457, 673, 793, 910
BseXI GCAGC 1 cut(s) 285
Bsh1285I CGRYCG 1 cut(s) 952
BshFI GGCC 4 cut(s) 132, 184, 485, 1027
BshNI GGYRCC 2 cut(s) 422, 671
BshTI ACCGGT 1 cut(s) 674
BsiEI CGRYCG 1 cut(s) 952
BsiHKAI GWGCWC 1 cut(s) 608
BsiHKCI CYCGRG 1 cut(s) 601
BsiSI CCGG 2 cut(s) 129, 675
BslFI GGGAC 2 cut(s) 505, 689
BslI CCNNNNNNNGG 2 cut(s) 393, 681
BsmAI GTCTC 4 cut(s) 130, 185, 443, 950
BsmFI GGGAC 2 cut(s) 505, 689
BsnI GGCC 4 cut(s) 132, 184, 485, 1027
Bso31I GGTCTC 1 cut(s) 950
BsoBI CYCGRG 1 cut(s) 601
Bsp1286I GDGCHC 1 cut(s) 608
Bsp1407I TGTACA 2 cut(s) 736, 777
Bsp143I GATC 7 cut(s) 229, 327, 476, 892, 979, 1006, 1105
Bsp19I CCATGG 1 cut(s) 1028
BspACI CCGC 1 cut(s) 523
BspANI GGCC 4 cut(s) 132, 184, 485, 1027
BspCNI CTCAG 4 cut(s) 263, 508, 900, 1076
BspLI GGNNCC 4 cut(s) 424, 673, 705, 894
BspPI GGATC 6 cut(s) 335, 484, 887, 900, 987, 1113
BspQI GCTCTTC 1 cut(s) 444
BspT107I GGYRCC 2 cut(s) 422, 671
BspTNI GGTCTC 1 cut(s) 950
BsrFI RCCGGY 1 cut(s) 674
BsrGI TGTACA 2 cut(s) 736, 777
BsrI ACTGG 7 cut(s) 152, 178, 342, 457, 673, 793, 910
BssAI RCCGGY 1 cut(s) 674
BssECI CCNNGG 3 cut(s) 1022, 1028, 1052
BssMI GATC 7 cut(s) 229, 327, 476, 892, 979, 1006, 1105
BssNAI GTATAC 1 cut(s) 680
BssSI CACGAG 1 cut(s) 201
BssT1I CCWWGG 3 cut(s) 1022, 1028, 1052
Bst1107I GTATAC 1 cut(s) 680
Bst2BI CACGAG 1 cut(s) 201
Bst2UI CCWGG 2 cut(s) 701, 897
Bst4CI ACNGT 2 cut(s) 196, 823
Bst6I CTCTTC 1 cut(s) 444
BstAPI GCANNNNNTGC 1 cut(s) 119
BstAUI TGTACA 2 cut(s) 736, 777
BstC8I GCNNGC 2 cut(s) 580, 631
BstDEI CTNAG 4 cut(s) 250, 516, 887, 1063
BstDSI CCRYGG 1 cut(s) 1028
BstEII GGTNACC 1 cut(s) 615
BstF5I GGATG 2 cut(s) 174, 1009
BstH2I RGCGCY 1 cut(s) 279
BstHHI GCGC 2 cut(s) 278, 386
BstKTI GATC 7 cut(s) 232, 330, 479, 895, 982, 1009, 1108
BstMAI GTCTC 4 cut(s) 130, 185, 443, 950
BstMBI GATC 7 cut(s) 229, 327, 476, 892, 979, 1006, 1105
BstMCI CGRYCG 1 cut(s) 952
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 2 cut(s) 701, 897
BstPI GGTNACC 1 cut(s) 615
BstSCI CCNGG 2 cut(s) 699, 895
BstSFI CTRYAG 1 cut(s) 570
BstV1I GCAGC 1 cut(s) 285
BstV2I GAAGAC 2 cut(s) 550, 889
BstX2I RGATCY 4 cut(s) 327, 476, 892, 1105
BstXI CCANNNNNNTGG 1 cut(s) 1029
BstYI RGATCY 4 cut(s) 327, 476, 892, 1105
BstZ17I GTATAC 1 cut(s) 680
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 4 cut(s) 132, 184, 485, 1027
BtgI CCRYGG 1 cut(s) 1028
BtsCI GGATG 2 cut(s) 174, 1009
BtsIMutI CAGTG 2 cut(s) 258, 335
Cac8I GCNNGC 2 cut(s) 580, 631
CfoI GCGC 2 cut(s) 278, 386
Cfr10I RCCGGY 1 cut(s) 674
Cfr13I GGNCC 2 cut(s) 703, 899
Csp6I GTAC 3 cut(s) 672, 737, 778
CspAI ACCGGT 1 cut(s) 674
CviAII CATG 3 cut(s) 280, 941, 1029
CviQI GTAC 3 cut(s) 672, 737, 778
DdeI CTNAG 4 cut(s) 250, 516, 887, 1063
DpnI GATC 7 cut(s) 231, 329, 478, 894, 981, 1008, 1107
DpnII GATC 7 cut(s) 229, 327, 476, 892, 979, 1006, 1105
EaeI YGGCCR 2 cut(s) 130, 483
Eam1104I CTCTTC 1 cut(s) 444
EarI CTCTTC 1 cut(s) 444
Ecl136II GAGCTC 1 cut(s) 606
Eco130I CCWWGG 3 cut(s) 1022, 1028, 1052
Eco147I AGGCCT 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 608
Eco31I GGTCTC 1 cut(s) 950
Eco47I GGWCC 2 cut(s) 703, 899
Eco53kI GAGCTC 1 cut(s) 606
Eco57I CTGAAG 2 cut(s) 276, 425
Eco88I CYCGRG 1 cut(s) 601
Eco91I GGTNACC 1 cut(s) 615
EcoICRI GAGCTC 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 703
EcoO65I GGTNACC 1 cut(s) 615
EcoRII CCWGG 2 cut(s) 699, 895
EcoT14I CCWWGG 3 cut(s) 1022, 1028, 1052
EcoT22I ATGCAT 1 cut(s) 1138
EcoT38I GRGCYC 1 cut(s) 608
ErhI CCWWGG 3 cut(s) 1022, 1028, 1052
FaeI CATG 3 cut(s) 283, 944, 1032
FaqI GGGAC 2 cut(s) 505, 689
FatI CATG 3 cut(s) 279, 940, 1028
FauI CCCGC 1 cut(s) 530
FblI GTMKAC 2 cut(s) 679, 952
Fnu4HI GCNGC 1 cut(s) 274
FokI GGATG 2 cut(s) 181, 996
FriOI GRGCYC 1 cut(s) 608
Fsp4HI GCNGC 1 cut(s) 274
FspBI CTAG 1 cut(s) 1071
GlaI GCGC 2 cut(s) 277, 385
GluI GCNGC 1 cut(s) 274
GsuI CTGGAG 2 cut(s) 338, 516
HaeII RGCGCY 1 cut(s) 279
HaeIII GGCC 4 cut(s) 132, 184, 485, 1027
HapII CCGG 2 cut(s) 129, 675
HhaI GCGC 2 cut(s) 278, 386
Hin1II CATG 3 cut(s) 283, 944, 1032
Hin6I GCGC 2 cut(s) 276, 384
HinP1I GCGC 2 cut(s) 276, 384
HincII GTYRAC 2 cut(s) 157, 953
HindII GTYRAC 2 cut(s) 157, 953
HinfI GANTC 7 cut(s) 4, 65, 376, 518, 599, 885, 1059
HpaII CCGG 2 cut(s) 129, 675
HphI GGTGA 3 cut(s) 224, 609, 1011
Hpy166II GTNNAC 4 cut(s) 149, 157, 680, 953
Hpy188I TCNGA 4 cut(s) 444, 660, 1066, 1105
Hpy188III TCNNGA 8 cut(s) 140, 203, 294, 515, 643, 706, 747, 834
Hpy8I GTNNAC 4 cut(s) 149, 157, 680, 953
HpyAV CCTTC 5 cut(s) 80, 655, 831, 927, 1023
HpyCH4III ACNGT 2 cut(s) 196, 823
HpyCH4V TGCA 3 cut(s) 629, 766, 1136
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 4 cut(s) 250, 516, 887, 1063
Hsp92II CATG 3 cut(s) 283, 944, 1032
HspAI GCGC 2 cut(s) 276, 384
KpnI GGTACC 1 cut(s) 675
Kzo9I GATC 7 cut(s) 229, 327, 476, 892, 979, 1006, 1105
LguI GCTCTTC 1 cut(s) 444
LmnI GCTCC 2 cut(s) 611, 1054
Lsp1109I GCAGC 1 cut(s) 285
LweI GCATC 2 cut(s) 760, 1043
MaeI CTAG 1 cut(s) 1071
MaeIII GTNAC 5 cut(s) 364, 615, 664, 795, 934
MalI GATC 7 cut(s) 231, 329, 478, 894, 981, 1008, 1107
MboI GATC 7 cut(s) 229, 327, 476, 892, 979, 1006, 1105
MboII GAAGA 7 cut(s) 42, 128, 431, 550, 894, 912, 1008
MflI RGATCY 4 cut(s) 327, 476, 892, 1105
MhlI GDGCHC 1 cut(s) 608
MluCI AATT 6 cut(s) 262, 504, 654, 751, 829, 1000
MlyI GAGTC 3 cut(s) 527, 593, 879
MmeI TCCRAC 2 cut(s) 451, 840
MnlI CCTC 8 cut(s) 210, 237, 310, 467, 568, 585, 630, 834
Mph1103I ATGCAT 1 cut(s) 1138
MroXI GAANNNNTTC 1 cut(s) 754
MseI TTAA 3 cut(s) 372, 503, 1092
MspI CCGG 2 cut(s) 129, 675
MspR9I CCNGG 2 cut(s) 701, 897
MvaI CCWGG 2 cut(s) 701, 897
MwoI GCNNNNNNNGC 1 cut(s) 119
NcoI CCATGG 1 cut(s) 1028
NdeII GATC 7 cut(s) 229, 327, 476, 892, 979, 1006, 1105
NlaIII CATG 3 cut(s) 283, 944, 1032
NlaIV GGNNCC 4 cut(s) 424, 673, 705, 894
NmeAIII GCCGAG 2 cut(s) 294, 511
NmuCI GTSAC 1 cut(s) 615
NsiI ATGCAT 1 cut(s) 1138
PaeR7I CTCGAG 1 cut(s) 601
PceI AGGCCT 1 cut(s) 184
PciSI GCTCTTC 1 cut(s) 444
PdmI GAANNNNTTC 1 cut(s) 754
PfeI GAWTC 4 cut(s) 4, 65, 376, 1059
PinAI ACCGGT 1 cut(s) 674
PkrI GCNGC 1 cut(s) 275
PleI GAGTC 3 cut(s) 526, 593, 879
PpsI GAGTC 3 cut(s) 526, 593, 879
PpuMI RGGWCCY 1 cut(s) 703
PshBI ATTAAT 1 cut(s) 503
Psp124BI GAGCTC 1 cut(s) 608
Psp5II RGGWCCY 1 cut(s) 703
Psp6I CCWGG 2 cut(s) 699, 895
PspEI GGTNACC 1 cut(s) 615
PspGI CCWGG 2 cut(s) 699, 895
PspN4I GGNNCC 4 cut(s) 424, 673, 705, 894
PspPI GGNCC 2 cut(s) 703, 899
PspPPI RGGWCCY 1 cut(s) 703
PspXI VCTCGAGB 1 cut(s) 601
PsuI RGATCY 4 cut(s) 327, 476, 892, 1105
RsaI GTAC 3 cut(s) 673, 738, 779
RsaNI GTAC 3 cut(s) 672, 737, 778
SacI GAGCTC 1 cut(s) 608
SalI GTCGAC 1 cut(s) 951
SapI GCTCTTC 1 cut(s) 444
SaqAI TTAA 3 cut(s) 372, 503, 1092
SatI GCNGC 1 cut(s) 274
Sau3AI GATC 7 cut(s) 229, 327, 476, 892, 979, 1006, 1105
Sau96I GGNCC 2 cut(s) 703, 899
SchI GAGTC 3 cut(s) 527, 593, 879
ScrFI CCNGG 2 cut(s) 701, 897
SduI GDGCHC 1 cut(s) 608
SfaNI GCATC 2 cut(s) 760, 1043
SfcI CTRYAG 1 cut(s) 570
Sfr274I CTCGAG 1 cut(s) 601
SinI GGWCC 2 cut(s) 703, 899
SlaI CTCGAG 1 cut(s) 601
SmlI CTYRAG 2 cut(s) 562, 601
SmoI CTYRAG 2 cut(s) 562, 601
Sse9I AATT 6 cut(s) 262, 504, 654, 751, 829, 1000
SseBI AGGCCT 1 cut(s) 184
SsiI CCGC 1 cut(s) 523
SspI AATATT 1 cut(s) 784
SspMI CTAG 1 cut(s) 1071
SstI GAGCTC 1 cut(s) 608
StuI AGGCCT 1 cut(s) 184
StyD4I CCNGG 2 cut(s) 699, 895
StyI CCWWGG 3 cut(s) 1022, 1028, 1052
TaaI ACNGT 2 cut(s) 196, 823
TaqI TCGA 2 cut(s) 602, 952
TaqII GACCGA 1 cut(s) 938
TasI AATT 6 cut(s) 262, 504, 654, 751, 829, 1000
TatI WGTACW 2 cut(s) 736, 777
TfiI GAWTC 4 cut(s) 4, 65, 376, 1059
Tru1I TTAA 3 cut(s) 372, 503, 1092
Tru9I TTAA 3 cut(s) 372, 503, 1092
TscAI CASTG 2 cut(s) 258, 342
TseFI GTSAC 1 cut(s) 615
TseI GCWGC 1 cut(s) 273
Tsp45I GTSAC 1 cut(s) 615
TspDTI ATGAA 2 cut(s) 510, 912
TspGWI ACGGA 1 cut(s) 697
TspRI CASTG 2 cut(s) 258, 342
VpaK11BI GGWCC 2 cut(s) 703, 899
VspI ATTAAT 1 cut(s) 503
XapI RAATTY 2 cut(s) 654, 751
XhoI CTCGAG 1 cut(s) 601
XmiI GTMKAC 2 cut(s) 679, 952
XmnI GAANNNNTTC 1 cut(s) 754
XspI CTAG 1 cut(s) 1071
Zsp2I ATGCAT 1 cut(s) 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.