Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0002957

Physical Location & Seq

Genomic Coordinates Forward (+)

17405855 .. 17407297

Gene Structure 1443 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG123600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1071 bp

ATGATGTGGCAGATAGAAGGGGATATTTTGGCTGTAAAGAAAGCACTTGTTGCTGTCTCCGGCCGTCTTCAAGATTGTCCACCAGTTGACAAGACAAGGATGACTGGAAGTAGGCCTATTGAGACAGTTCCTCGTGAAACTTTACCTGATATTCGTGTAGATCACCTTTCACAGAGGAACTCAGTGATTTCCAATTTGCCGAGCAGCTCCATGAGTTATGCTTCAGGAGTTTGTCTGCCATCAATAGAGGCTGAAAGGATCTCAACACTGGATACAAGAACACTCCAGCAGGAAGTTACTTTTAAGATTCTTTGCGACAATGATAGGGTTGGGGGTGTCATTGGAAAAGGGGGTGCCATTGTCAAGGCTCTTCAGAATGAGACTGGTGCTACTATAAGTGTTGGAGGATCTGTGGCCGAGTGTGATGAAAGATTAATTACTAATCCTGAGTCCCGCTACTCTCCAGCACAAAAGGGTCTTGTTCTTGTCTTCTCAAGGTCTGTAGAGGCAGGCATTGAAAAGGGGCAAGACTCGAGCTCCAAAGGGTCACCTCTTATTGCACGCCTTGTTGTCCCGACAAGCCAAATTCGGAAGGTAACTGGTACCGGTATACGGATAATAGGTGGTGACCAGGTCCCGAAGTGTGCCGTAGAGAATGACGAAGTTGTACAGGTGTCAAGAAATTTTTCAAGTGTGCAAGATGCTTTGTACAATATTACTGGTAGGTTACGAGATAACCTTTTCTCCAACACAGTAAATAATTCTGGAAGGAGGAATAGTTCAGCCGTGCTAACAGATACCAATCCTTATGGAAGACTCAGGGATCCTGCTCCTACTGGATTTCAGCCTTCTTCATCAGTTGGTGGTACTCATGGTCTCGGTCGACATACTACTCTAACTCAAAGTATGGATCATCTTGGACTTTCCCATAATTTAGATCATCCTTCTTCACCAAGGCCATGGGCATCACAGATGGTTGCTCCAAGGGGAATCTCAGATACTAGCAGGGGTTTGACTTCTCTTAAAAGTGGCTCGGATCTTGGTAGGTTAGTAATGTCGCACAATGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0007275 GO:0008150 GO:0009791 GO:0009908 GO:0009909 GO:0009911 GO:0016604 GO:0016607 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045596 GO:0048367 GO:0048437 GO:0048497 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051239 GO:0051240 GO:0061458 GO:0065007 GO:0070013 GO:0090567 GO:0090700 GO:0099402 GO:2000026 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KH_1

Protein Analysis

356

Amino Acids

38.05

Weight (kDa)

9.01

Isoelectric Point (pI)

49.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KH_1	PF00013	100 - 147	2.1e-10	KH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002957)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64390 AT5G64390 AT5G64390
fragaria_vesca	FvH4_2g11560 FvH4_2g11560 FvH4_2g11560
malus_domestica	MD05G1048400.v1.1 MD10G1054800.v1.1
prunus_persica	Prupe.8G070900_v2.0.a1
pyrus_communis	pycom05g04000 pycom10g04180
rosa_chinensis	RchiOBHm_Chr1g0353311 RchiOBHm_Chr4g0385021 RchiOBHm_Chr6g0270731 RchiOBHm_Chr6g0272811
rosa_laevigata	RLG00000013774
rosa_multiflora	Rmu_co8284529.1_g000001 Rmu_sc0000642.1_g000012 Rmu_sc0041041.1_g000001
rosa_roxburghii	Rroxscaffold_2G00085770 Rroxscaffold_7G00197640
rosa_rugosa	Rorug06G0056000 Rorug06G0056100 Rorug06G0056200
rosa_samantha	Rh2DG204000 Rh4CG002400 Rh4CG168900 Rh6AG157400 Rh6AG175200 Rh6BG140200 Rh6BG178600 Rh6CG174800 Rh6DG123600 Rh6DG167400 Rh6DG180500 Rh7AG209600 Rh7CG359700
rosa_wichuraiana	Rw6G014930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	602
AccB1I	GGYRCC	2 cut(s)	353, 602
AccI	GTMKAC	2 cut(s)	610, 883
AciI	CCGC	1 cut(s)	454
AclWI	GGATC	6 cut(s)	266, 415, 818, 831, 918, 1044
AcoI	YGGCCR	2 cut(s)	61, 414
AcsI	RAATTY	2 cut(s)	585, 682
AcuI	CTGAAG	2 cut(s)	207, 356
AfaI	GTAC	4 cut(s)	604, 669, 710, 868
AfiI	CCNNNNNNNGG	1 cut(s)	612
AgeI	ACCGGT	1 cut(s)	605
AgsI	TTSAA	3 cut(s)	71, 518, 690
AjnI	CCWGG	1 cut(s)	630
AloI	GAACNNNNNNTCC	2 cut(s)	763, 795
AluBI	AGCT	2 cut(s)	207, 537
AluI	AGCT	2 cut(s)	207, 537
Alw21I	GWGCWC	1 cut(s)	539
Alw26I	GTCTC	4 cut(s)	61, 116, 374, 881
AlwI	GGATC	6 cut(s)	266, 415, 818, 831, 918, 1044
Ama87I	CYCGRG	1 cut(s)	532
AoxI	GGCC	4 cut(s)	61, 113, 414, 956
ApeKI	GCWGC	1 cut(s)	204
ApoI	RAATTY	2 cut(s)	585, 682
AseI	ATTAAT	1 cut(s)	434
AsiGI	ACCGGT	1 cut(s)	605
Asp700I	GAANNNNTTC	1 cut(s)	685
Asp718I	GGTACC	1 cut(s)	602
AspS9I	GGNCC	1 cut(s)	634
AsuHPI	GGTGA	4 cut(s)	155, 540, 638, 942
AvaI	CYCGRG	1 cut(s)	532
AvaII	GGWCC	1 cut(s)	634
BamHI	GGATCC	1 cut(s)	823
BanI	GGYRCC	2 cut(s)	353, 602
BanII	GRGCYC	1 cut(s)	539
BauI	CACGAG	1 cut(s)	132
BbsI	GAAGAC	3 cut(s)	59, 481, 820
Bbv12I	GWGCWC	1 cut(s)	539
BbvI	GCAGC	1 cut(s)	216
BccI	CCATC	2 cut(s)	247, 967
BceAI	ACGGC	3 cut(s)	48, 632, 770
BciT130I	CCWGG	1 cut(s)	632
BciVI	GTATCC	1 cut(s)	265
BcoDI	GTCTC	4 cut(s)	61, 116, 374, 881
BfaI	CTAG	1 cut(s)	1002
BfmI	CTRYAG	1 cut(s)	501
BfuI	GTATCC	1 cut(s)	265
BisI	GCNGC	1 cut(s)	205
BlsI	GCNGC	1 cut(s)	206
Bme1390I	CCNGG	1 cut(s)	632
Bme18I	GGWCC	1 cut(s)	634
BmeT110I	CYCGRG	1 cut(s)	532
BmgT120I	GGNCC	1 cut(s)	634
BmiI	GGNNCC	4 cut(s)	355, 604, 636, 825
BmrFI	CCNGG	1 cut(s)	632
BmsI	GCATC	2 cut(s)	691, 974
BpiI	GAAGAC	3 cut(s)	59, 481, 820
BpmI	CTGGAG	2 cut(s)	269, 447
BpuEI	CTTGAG	1 cut(s)	478
BsaBI	GATNNNNATC	1 cut(s)	801
BsaI	GGTCTC	1 cut(s)	881
BsaJI	CCNNGG	3 cut(s)	953, 959, 983
BsaWI	WCCGGW	1 cut(s)	605
BsaXI	ACNNNNNCTCC	8 cut(s)	396, 426, 521, 551, 728, 758, 763, 793
Bsc4I	CCNNNNNNNGG	1 cut(s)	612
Bse118I	RCCGGY	1 cut(s)	605
Bse1I	ACTGG	7 cut(s)	83, 109, 273, 388, 604, 724, 841
Bse8I	GATNNNNATC	1 cut(s)	801
BseBI	CCWGG	1 cut(s)	632
BseDI	CCNNGG	3 cut(s)	953, 959, 983
BseGI	GGATG	2 cut(s)	105, 940
BseJI	GATNNNNATC	1 cut(s)	801
BseLI	CCNNNNNNNGG	1 cut(s)	612
BseMII	CTCAG	4 cut(s)	195, 438, 832, 1008
BseNI	ACTGG	7 cut(s)	83, 109, 273, 388, 604, 724, 841
BseX3I	CGGCCG	1 cut(s)	61
BseXI	GCAGC	1 cut(s)	216
Bsh1285I	CGRYCG	2 cut(s)	64, 883
BshFI	GGCC	4 cut(s)	63, 115, 416, 958
BshNI	GGYRCC	2 cut(s)	353, 602
BshTI	ACCGGT	1 cut(s)	605
BsiEI	CGRYCG	2 cut(s)	64, 883
BsiHKAI	GWGCWC	1 cut(s)	539
BsiHKCI	CYCGRG	1 cut(s)	532
BsiSI	CCGG	2 cut(s)	60, 606
BslFI	GGGAC	3 cut(s)	436, 557, 620
BslI	CCNNNNNNNGG	1 cut(s)	612
BsmAI	GTCTC	4 cut(s)	61, 116, 374, 881
BsmFI	GGGAC	3 cut(s)	436, 557, 620
BsnI	GGCC	4 cut(s)	63, 115, 416, 958
Bso31I	GGTCTC	1 cut(s)	881
BsoBI	CYCGRG	1 cut(s)	532
Bsp1286I	GDGCHC	1 cut(s)	539
Bsp1407I	TGTACA	2 cut(s)	667, 708
Bsp143I	GATC	7 cut(s)	160, 258, 407, 823, 910, 937, 1036
Bsp19I	CCATGG	1 cut(s)	959
BspACI	CCGC	1 cut(s)	454
BspANI	GGCC	4 cut(s)	63, 115, 416, 958
BspCNI	CTCAG	4 cut(s)	194, 439, 831, 1007
BspLI	GGNNCC	4 cut(s)	355, 604, 636, 825
BspPI	GGATC	6 cut(s)	266, 415, 818, 831, 918, 1044
BspQI	GCTCTTC	1 cut(s)	375
BspT107I	GGYRCC	2 cut(s)	353, 602
BspTNI	GGTCTC	1 cut(s)	881
BsrFI	RCCGGY	1 cut(s)	605
BsrGI	TGTACA	2 cut(s)	667, 708
BsrI	ACTGG	7 cut(s)	83, 109, 273, 388, 604, 724, 841
BssAI	RCCGGY	1 cut(s)	605
BssECI	CCNNGG	3 cut(s)	953, 959, 983
BssMI	GATC	7 cut(s)	160, 258, 407, 823, 910, 937, 1036
BssNAI	GTATAC	1 cut(s)	611
BssSI	CACGAG	1 cut(s)	132
BssT1I	CCWWGG	3 cut(s)	953, 959, 983
Bst1107I	GTATAC	1 cut(s)	611
Bst2BI	CACGAG	1 cut(s)	132
Bst2UI	CCWGG	1 cut(s)	632
Bst4CI	ACNGT	2 cut(s)	127, 754
Bst6I	CTCTTC	1 cut(s)	375
BstAPI	GCANNNNNTGC	1 cut(s)	50
BstAUI	TGTACA	2 cut(s)	667, 708
BstC8I	GCNNGC	2 cut(s)	511, 562
BstDEI	CTNAG	4 cut(s)	181, 447, 818, 994
BstDSI	CCRYGG	1 cut(s)	959
BstEII	GGTNACC	2 cut(s)	546, 626
BstF5I	GGATG	2 cut(s)	105, 940
BstKTI	GATC	7 cut(s)	163, 261, 410, 826, 913, 940, 1039
BstMAI	GTCTC	4 cut(s)	61, 116, 374, 881
BstMBI	GATC	7 cut(s)	160, 258, 407, 823, 910, 937, 1036
BstMCI	CGRYCG	2 cut(s)	64, 883
BstMWI	GCNNNNNNNGC	1 cut(s)	50
BstNI	CCWGG	1 cut(s)	632
BstPI	GGTNACC	2 cut(s)	546, 626
BstSCI	CCNGG	1 cut(s)	630
BstSFI	CTRYAG	1 cut(s)	501
BstV1I	GCAGC	1 cut(s)	216
BstV2I	GAAGAC	3 cut(s)	59, 481, 820
BstX2I	RGATCY	4 cut(s)	258, 407, 823, 1036
BstXI	CCANNNNNNTGG	1 cut(s)	960
BstYI	RGATCY	4 cut(s)	258, 407, 823, 1036
BstZ17I	GTATAC	1 cut(s)	611
BstZI	CGGCCG	1 cut(s)	61
BsuI	GTATCC	1 cut(s)	265
BsuRI	GGCC	4 cut(s)	63, 115, 416, 958
BtgI	CCRYGG	1 cut(s)	959
BtsCI	GGATG	2 cut(s)	105, 940
BtsIMutI	CAGTG	2 cut(s)	189, 266
Cac8I	GCNNGC	2 cut(s)	511, 562
Cfr10I	RCCGGY	1 cut(s)	605
Cfr13I	GGNCC	1 cut(s)	634
CsiI	ACCWGGT	1 cut(s)	630
Csp6I	GTAC	4 cut(s)	603, 668, 709, 867
CspAI	ACCGGT	1 cut(s)	605
CviAII	CATG	3 cut(s)	211, 872, 960
CviQI	GTAC	4 cut(s)	603, 668, 709, 867
DdeI	CTNAG	4 cut(s)	181, 447, 818, 994
DpnI	GATC	7 cut(s)	162, 260, 409, 825, 912, 939, 1038
DpnII	GATC	7 cut(s)	160, 258, 407, 823, 910, 937, 1036
EaeI	YGGCCR	2 cut(s)	61, 414
EagI	CGGCCG	1 cut(s)	61
Eam1104I	CTCTTC	1 cut(s)	375
EarI	CTCTTC	1 cut(s)	375
Ecl136II	GAGCTC	1 cut(s)	537
EclXI	CGGCCG	1 cut(s)	61
Eco130I	CCWWGG	3 cut(s)	953, 959, 983
Eco147I	AGGCCT	1 cut(s)	115
Eco24I	GRGCYC	1 cut(s)	539
Eco31I	GGTCTC	1 cut(s)	881
Eco47I	GGWCC	1 cut(s)	634
Eco52I	CGGCCG	1 cut(s)	61
Eco53kI	GAGCTC	1 cut(s)	537
Eco57I	CTGAAG	2 cut(s)	207, 356
Eco88I	CYCGRG	1 cut(s)	532
Eco91I	GGTNACC	2 cut(s)	546, 626
EcoICRI	GAGCTC	1 cut(s)	537
EcoO109I	RGGNCCY	1 cut(s)	634
EcoO65I	GGTNACC	2 cut(s)	546, 626
EcoRII	CCWGG	1 cut(s)	630
EcoT14I	CCWWGG	3 cut(s)	953, 959, 983
EcoT22I	ATGCAT	1 cut(s)	1069
EcoT38I	GRGCYC	1 cut(s)	539
ErhI	CCWWGG	3 cut(s)	953, 959, 983
FaeI	CATG	3 cut(s)	214, 875, 963
FaqI	GGGAC	3 cut(s)	436, 557, 620
FatI	CATG	3 cut(s)	210, 871, 959
FauI	CCCGC	1 cut(s)	461
FblI	GTMKAC	2 cut(s)	610, 883
Fnu4HI	GCNGC	1 cut(s)	205
FokI	GGATG	2 cut(s)	112, 927
FriOI	GRGCYC	1 cut(s)	539
Fsp4HI	GCNGC	1 cut(s)	205
FspBI	CTAG	1 cut(s)	1002
GluI	GCNGC	1 cut(s)	205
GsuI	CTGGAG	2 cut(s)	269, 447
HaeIII	GGCC	4 cut(s)	63, 115, 416, 958
HapII	CCGG	2 cut(s)	60, 606
Hin1II	CATG	3 cut(s)	214, 875, 963
HincII	GTYRAC	2 cut(s)	88, 884
HindII	GTYRAC	2 cut(s)	88, 884
HinfI	GANTC	5 cut(s)	307, 449, 530, 816, 990
HpaII	CCGG	2 cut(s)	60, 606
HphI	GGTGA	4 cut(s)	155, 540, 638, 942
Hpy166II	GTNNAC	4 cut(s)	80, 88, 611, 884
Hpy188I	TCNGA	4 cut(s)	375, 591, 997, 1036
Hpy188III	TCNNGA	8 cut(s)	71, 134, 225, 446, 574, 637, 678, 765
Hpy8I	GTNNAC	4 cut(s)	80, 88, 611, 884
HpyAV	CCTTC	5 cut(s)	11, 586, 762, 858, 954
HpyCH4III	ACNGT	2 cut(s)	127, 754
HpyCH4V	TGCA	3 cut(s)	560, 697, 1067
HpyF10VI	GCNNNNNNNGC	1 cut(s)	50
HpyF3I	CTNAG	4 cut(s)	181, 447, 818, 994
Hsp92II	CATG	3 cut(s)	214, 875, 963
KpnI	GGTACC	1 cut(s)	606
Kzo9I	GATC	7 cut(s)	160, 258, 407, 823, 910, 937, 1036
LguI	GCTCTTC	1 cut(s)	375
LmnI	GCTCC	4 cut(s)	212, 542, 835, 985
Lsp1109I	GCAGC	1 cut(s)	216
LweI	GCATC	2 cut(s)	691, 974
MabI	ACCWGGT	1 cut(s)	630
MaeI	CTAG	1 cut(s)	1002
MaeIII	GTNAC	5 cut(s)	295, 546, 595, 626, 726
MalI	GATC	7 cut(s)	162, 260, 409, 825, 912, 939, 1038
MboI	GATC	7 cut(s)	160, 258, 407, 823, 910, 937, 1036
MboII	GAAGA	6 cut(s)	59, 362, 481, 825, 843, 939
MflI	RGATCY	4 cut(s)	258, 407, 823, 1036
MhlI	GDGCHC	1 cut(s)	539
MluCI	AATT	6 cut(s)	193, 435, 585, 682, 760, 931
MlyI	GAGTC	3 cut(s)	458, 524, 810
MmeI	TCCRAC	2 cut(s)	382, 771
MnlI	CCTC	7 cut(s)	141, 168, 241, 398, 499, 561, 765
Mph1103I	ATGCAT	1 cut(s)	1069
MroXI	GAANNNNTTC	1 cut(s)	685
MseI	TTAA	3 cut(s)	303, 434, 1023
MspI	CCGG	2 cut(s)	60, 606
MspR9I	CCNGG	1 cut(s)	632
MvaI	CCWGG	1 cut(s)	632
MwoI	GCNNNNNNNGC	1 cut(s)	50
NcoI	CCATGG	1 cut(s)	959
NdeII	GATC	7 cut(s)	160, 258, 407, 823, 910, 937, 1036
NlaIII	CATG	3 cut(s)	214, 875, 963
NlaIV	GGNNCC	4 cut(s)	355, 604, 636, 825
NmeAIII	GCCGAG	2 cut(s)	225, 442
NmuCI	GTSAC	2 cut(s)	546, 626
NsiI	ATGCAT	1 cut(s)	1069
PaeR7I	CTCGAG	1 cut(s)	532
PceI	AGGCCT	1 cut(s)	115
PciSI	GCTCTTC	1 cut(s)	375
PdmI	GAANNNNTTC	1 cut(s)	685
PfeI	GAWTC	2 cut(s)	307, 990
PflFI	GACNNNGTC	1 cut(s)	632
PinAI	ACCGGT	1 cut(s)	605
PkrI	GCNGC	1 cut(s)	206
PleI	GAGTC	3 cut(s)	457, 524, 810
PpsI	GAGTC	3 cut(s)	457, 524, 810
PpuMI	RGGWCCY	1 cut(s)	634
PshBI	ATTAAT	1 cut(s)	434
Psp124BI	GAGCTC	1 cut(s)	539
Psp5II	RGGWCCY	1 cut(s)	634
Psp6I	CCWGG	1 cut(s)	630
PspEI	GGTNACC	2 cut(s)	546, 626
PspGI	CCWGG	1 cut(s)	630
PspN4I	GGNNCC	4 cut(s)	355, 604, 636, 825
PspPI	GGNCC	1 cut(s)	634
PspPPI	RGGWCCY	1 cut(s)	634
PspXI	VCTCGAGB	1 cut(s)	532
PsuI	RGATCY	4 cut(s)	258, 407, 823, 1036
PsyI	GACNNNGTC	1 cut(s)	632
RsaI	GTAC	4 cut(s)	604, 669, 710, 868
RsaNI	GTAC	4 cut(s)	603, 668, 709, 867
SacI	GAGCTC	1 cut(s)	539
SalI	GTCGAC	1 cut(s)	882
SapI	GCTCTTC	1 cut(s)	375
SaqAI	TTAA	3 cut(s)	303, 434, 1023
SatI	GCNGC	1 cut(s)	205
Sau3AI	GATC	7 cut(s)	160, 258, 407, 823, 910, 937, 1036
Sau96I	GGNCC	1 cut(s)	634
SchI	GAGTC	3 cut(s)	458, 524, 810
ScrFI	CCNGG	1 cut(s)	632
SduI	GDGCHC	1 cut(s)	539
SexAI	ACCWGGT	1 cut(s)	630
SfaNI	GCATC	2 cut(s)	691, 974
SfcI	CTRYAG	1 cut(s)	501
Sfr274I	CTCGAG	1 cut(s)	532
SinI	GGWCC	1 cut(s)	634
SlaI	CTCGAG	1 cut(s)	532
SmlI	CTYRAG	2 cut(s)	493, 532
SmoI	CTYRAG	2 cut(s)	493, 532
Sse9I	AATT	6 cut(s)	193, 435, 585, 682, 760, 931
SseBI	AGGCCT	1 cut(s)	115
SsiI	CCGC	1 cut(s)	454
SspI	AATATT	1 cut(s)	715
SspMI	CTAG	1 cut(s)	1002
SstI	GAGCTC	1 cut(s)	539
StuI	AGGCCT	1 cut(s)	115
StyD4I	CCNGG	1 cut(s)	630
StyI	CCWWGG	3 cut(s)	953, 959, 983
TaaI	ACNGT	2 cut(s)	127, 754
TaqI	TCGA	2 cut(s)	533, 883
TaqII	GACCGA	1 cut(s)	869
TasI	AATT	6 cut(s)	193, 435, 585, 682, 760, 931
TatI	WGTACW	2 cut(s)	667, 708
TfiI	GAWTC	2 cut(s)	307, 990
Tru1I	TTAA	3 cut(s)	303, 434, 1023
Tru9I	TTAA	3 cut(s)	303, 434, 1023
TscAI	CASTG	2 cut(s)	189, 273
TseFI	GTSAC	2 cut(s)	546, 626
TseI	GCWGC	1 cut(s)	204
Tsp45I	GTSAC	2 cut(s)	546, 626
TspDTI	ATGAA	2 cut(s)	441, 843
TspGWI	ACGGA	1 cut(s)	628
TspRI	CASTG	2 cut(s)	189, 273
Tth111I	GACNNNGTC	1 cut(s)	632
VpaK11BI	GGWCC	1 cut(s)	634
VspI	ATTAAT	1 cut(s)	434
XapI	RAATTY	2 cut(s)	585, 682
XhoI	CTCGAG	1 cut(s)	532
XmiI	GTMKAC	2 cut(s)	610, 883
XmnI	GAANNNNTTC	1 cut(s)	685
XspI	CTAG	1 cut(s)	1002
Zsp2I	ATGCAT	1 cut(s)	1069

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.