Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003895

Physical Location & Seq

Genomic Coordinates Forward (+)

8755836 .. 8767302

Gene Structure 11467 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1050600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2451 bp

ATGTTGGAGTCGGTGACTTATATCGGAAGCTCTAAGGGATACAGCACTATCAAACAAAAGAGCCCATCTATGGCCTGCTCTCTATCCAGATCACGGCTTTCTGGGCGTTCTTTTATTCCGTATTCCAGTAACCATCAAGTGCATCCATTATCATATGCCACTAATTACAACACAAGGCATCCCCCTTTTGCTCCAAGAAACTTTTTGGGAAATCCTTTACTAGCTTCCTCTGTCTGCGGATGGAGAGGACTTTATCTCTCAAACCACAGACCAGTCCATTCAAAAAGATTGCGAACGCATGCAGGACTTGATGTTGCGAGTGCTGTTGATGTCATTAATGATTTGGGATTTGATACTTTGACGTTCTTAGCTGTTACTGTCATTATCGTTCCGGCATTCAAGATCATTAAAGCTAGCCCTATACTTGGTTTCTTTTTTGCGGGGATTGTACTCAATCAATTTGGCTTGATTCGGAATCTTACAGATGTGAAAGTTTTGTCTGAATGGGGGATTCTTTTCTTGCTGTTTGAGATGGGCTTGGAGCTTTCATTTTCGCGTCTAAAGGCTCTTGCAAAATATGCCTTCGGAATGGGATTAACTCAGGTCGTATTATCTACTCTTGCTTTCACAGCCTTCGAACTTCCACCTAATGGTGCTATTGGAACCCAAATTTTGACGTTCCTCTTTAACTCAAGGCCTGATTTGGTCAACATCAGAAGCATTGATGAAGCCGTAGTGATTGGTGCTGCTCTCTCTCTGTCTTCTTCAGCTTTTGTACTTCAGCTACTTGCAGAGAAAGGTGAACTCCCTACAAGATTTGGGTCAGCAACTCTAGGGATACTTCTTCTACAGGACATAGCAGTCGTACCTCTCTTAGTCATACTTCCAGTGCTGGAAAGTCAGAACCTAGCAGAGGAAAGTATATGGCCAATGCTTGTGAAGGAAAGCCTAAAAGCCTTAGGTGGACTTGGTATTCTTTCTCTTGGAGGAAAATTCCTTCTTAGACGAGTTTTTGAGTTTGTGGCAGAAGCAAGGAGCTCTGAGGCTTTTGTTGCACTTTGCTTATTGACAGTTGCAGGGACCTCACTTCTCACCCAGAAGTTGGGTTTCAGTGATACGCTTGGAGCTTTTTTGGCTGGAGCACTGTTAGCGGAAACAAATTTCCGGACACAAATTGAAGCTGATATAAGGCCATTTCGAGGCTTACTTCTTGGATTATTTTTTGTAACTACAGGGACGTCCATTGACATGCCGCTTCTGTTCCGAGAGTGGCCGAATGTGCTTTCGCTCTTGGGAGGTTTGATTGTCATCAAGACACTGATAATAACAGTGATAGGCCCTCGTGTTGGACTTACCTTACAGGAAAGTGTAAGAATAGGGCTGCTTCTATCTCAAGGAGGCGAGTTTGGATTTGTTGTTTTTTCACTTGCAAATAGGCTTGGTGTGCTGCCACTCGAGCTTAACAAGTTGCTTATAATTGTTGTTGTCTTGTCAATGGCATTGACCCCATTGCTTAATGAAGCTGGAAGAAGGGCTGCTGAATTGATTGATGACAAATTTGGTGCCGAGGATAAAGCTGCTGAGGTGGTGAACTTTGATTCAAGTGAACCTGTTGTTATTCTTGGATTTGGACAAATGGGCCAGGTCTCAGATCCAATTGTCCTTGCCAATTTCTTGTCCACTCCATTGGCGTCTGGGATAGATAACGATAATCTGGGATTGCCGTTTGTAGCTTTTGATATGGATCCTTCTGTGGTGAAGGCATCTAGGAAACTTGGTTTTCCAATTTTGTATGGGGATGGATCACGTCCAGCAGTTTTGCAATCTGCTGGCATCTCTTCCCCCAAAGCTGTCATGGTTTTATACACTGCGAGGAGTAGAACTACTGAGGCCGTTCAAAGGCTTCGACATGCTTTCCCAGCGATCCCTATTTATGCCAGAGCTCTGGATCTCAAGCACCTTTTAGAACTGAAGAAATCAGGTGCAACAGATGCTATTCTGGAAAGCGCAGAGATGAGTTTGCAGCTGGGCTCCAAGCTTTTGAAAGGGTTTGGAGTTAGGTCTGATGACGTGAACTTTCTTCGTCAACTCATTCGGGATTCTATGGAGCTGCAAGCTCAAGGAGCAAGCAAGACTGATGAGAAAGAACTTAATGATCTGCAGCCTATGCAGGTGAGAGTTGCGGACTTAATTGATGACGCTGTACCCCTTTCAGCAACTCCATCAGAAGACAAATCATGGGGGTTAAACGAGGAAGATGCTTCTTATAGCTTGACATTTGAGGGGGATGTGGATGCAGCAAAGCATGACAGTGAGCTCCACCGGTCAGAGCATACAGAAGAAAACGAAGAAGTTTCACACCGTGGCTTTGATACAGAGAACGGTTTTGCAATGAAATCACAAGACATTGAGGGATCTACCTCTTCCGTAACAACAAAGGATGAACCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006996 GO:0008150 GO:0008324 GO:0009314 GO:0009416 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009628 GO:0009642 GO:0009643 GO:0009657 GO:0009658 GO:0009893 GO:0009987 GO:0010109 GO:0015075 GO:0015318 GO:0016020 GO:0016043 GO:0019222 GO:0022857 GO:0022890 GO:0031323 GO:0031325 GO:0031976 GO:0031984 GO:0034220 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0065007 GO:0071840 GO:0098655 GO:1905157

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger TrkA_N

Protein Analysis

817

Amino Acids

88.34

Weight (kDa)

5.51

Isoelectric Point (pI)

39.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	125 - 506	1.2e-67	Sodium/hydrogen exchanger, transmembrane
TrkA_N	PF02254	569 - 666	1.4e-13	TrkA-N domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003895)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01790 AT1G01790 AT4G04850 AT4G04850
fragaria_vesca	FvH4_2g11000
malus_domestica	MD05G1050600.v1.1 MD10G1058200.v1.1
prunus_persica	Prupe.8G077000_v2.0.a1 Prupe.8G077000_v2.0.a1
pyrus_communis	pycom05g04260 pycom05g04270 pycom10g04490
rosa_chinensis	RchiOBHm_Chr6g0269141 RchiOBHm_Chr6g0269211 RchiOBHm_Chr7g0201951
rosa_laevigata	RLG00000017131
rosa_multiflora	Rmu_co8385553.1_g000001 Rmu_sc0005353.1_g000001
rosa_roxburghii	Rroxscaffold_3G00238290 Rroxscaffold_7G00198940
rosa_rugosa	Rorug05G0148000 Rorug05G0148100 Rorug06G0046600 Rorug06G0046600
rosa_samantha	Rh4DG004100 Rh6AG166300 Rh6BG171900 Rh6CG164800 Rh6CG165400 Rh6DG156300 Rh6DG156400 Rh6DG157000 Rh7AG286500
rosa_wichuraiana	Rw6G014360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1475
AarI	CACCTGC	1 cut(s)	2161
AasI	GACNNNNNNGTC	1 cut(s)	860
AatII	GACGTC	1 cut(s)	1241
Acc36I	ACCTGC	1 cut(s)	2161
AccB1I	GGYRCC	1 cut(s)	1562
AccB7I	CCANNNNNTGG	2 cut(s)	650, 1102
AccII	CGCG	1 cut(s)	556
AccIII	TCCGGA	1 cut(s)	1164
AciI	CCGC	5 cut(s)	237, 440, 1151, 1253, 2183
AclWI	GGATC	7 cut(s)	1646, 1739, 1752, 1810, 1918, 1956, 2422
AcoI	YGGCCR	2 cut(s)	926, 1271
AcsI	RAATTY	4 cut(s)	669, 992, 1159, 1556
AcuI	CTGAAG	3 cut(s)	750, 764, 1991
AcyI	GRCGYC	2 cut(s)	1238, 1691
AdeI	CACNNNGTG	1 cut(s)	2363
AfaI	GTAC	4 cut(s)	450, 777, 867, 2205
AfiI	CCNNNNNNNGG	9 cut(s)	70, 93, 425, 650, 913, 1102, 1199, 1346, 1899
AgeI	ACCGGT	1 cut(s)	2322
AgsI	TTSAA	6 cut(s)	282, 400, 1178, 1602, 1898, 2044
AjiI	CACGTC	2 cut(s)	1808, 2071
AjnI	CCWGG	1 cut(s)	1641
Alw21I	GWGCWC	4 cut(s)	1040, 1144, 1945, 2319
Alw26I	GTCTC	1 cut(s)	1651
AlwI	GGATC	7 cut(s)	1646, 1739, 1752, 1810, 1918, 1956, 2422
Ama87I	CYCGRG	1 cut(s)	1454
Aor13HI	TCCGGA	1 cut(s)	1164
AoxI	GGCC	8 cut(s)	72, 695, 926, 1190, 1271, 1336, 1639, 1890
ApeKI	GCWGC	9 cut(s)	746, 1381, 1447, 1535, 1577, 2023, 2110, 2161, 2297
ApoI	RAATTY	4 cut(s)	669, 992, 1159, 1556
AseI	ATTAAT	1 cut(s)	336
AsiGI	ACCGGT	1 cut(s)	2322
AspLEI	GCGC	1 cut(s)	2009
AspS9I	GGNCC	3 cut(s)	1080, 1337, 1639
AsuHPI	GGTGA	6 cut(s)	25, 812, 1084, 1600, 1768, 2185
AsuII	TTCGAA	1 cut(s)	636
AsuNHI	GCTAGC	1 cut(s)	413
AvaI	CYCGRG	1 cut(s)	1454
AvaII	GGWCC	1 cut(s)	1080
AxyI	CCTNAGG	1 cut(s)	958
BalI	TGGCCA	1 cut(s)	928
BamHI	GGATCC	1 cut(s)	1744
BanI	GGYRCC	1 cut(s)	1562
BanII	GRGCYC	5 cut(s)	65, 1040, 1945, 2033, 2319
BauI	CACGAG	1 cut(s)	1341
BbsI	GAAGAC	2 cut(s)	753, 2235
Bbv12I	GWGCWC	4 cut(s)	1040, 1144, 1945, 2319
BbvCI	CCTCAGC	1 cut(s)	1581
BbvI	GCAGC	9 cut(s)	733, 1368, 1434, 1522, 1564, 2035, 2097, 2173, 2309
BccI	CCATC	6 cut(s)	73, 141, 234, 526, 1793, 2230
BceAI	ACGGC	4 cut(s)	110, 716, 1708, 1877
BcgI	CGANNNNNNTGC	2 cut(s)	2240, 2274
BciT130I	CCWGG	1 cut(s)	1643
BciVI	GTATCC	2 cut(s)	32, 831
BcoDI	GTCTC	1 cut(s)	1651
BfaI	CTAG	5 cut(s)	221, 414, 833, 908, 1767
BfmI	CTRYAG	3 cut(s)	848, 1230, 2159
BfuAI	ACCTGC	1 cut(s)	2161
BfuI	GTATCC	2 cut(s)	32, 831
Bme1390I	CCNGG	1 cut(s)	1643
Bme18I	GGWCC	1 cut(s)	1080
BmeT110I	CYCGRG	1 cut(s)	1454
BmgBI	CACGTC	2 cut(s)	1808, 2071
BmgT120I	GGNCC	3 cut(s)	1080, 1337, 1639
BmiI	GGNNCC	5 cut(s)	664, 1081, 1564, 1746, 2032
BmrFI	CCNGG	1 cut(s)	1643
BmsI	GCATC	7 cut(s)	151, 187, 1772, 1842, 1981, 2248, 2284
BmtI	GCTAGC	1 cut(s)	417
BpiI	GAAGAC	2 cut(s)	753, 2235
BpmI	CTGGAG	1 cut(s)	1158
Bpu10I	CCTNAGC	1 cut(s)	1581
Bpu14I	TTCGAA	1 cut(s)	636
BpuEI	CTTGAG	4 cut(s)	676, 1377, 1937, 2103
BsaBI	GATNNNNATC	2 cut(s)	1307, 1743
BsaHI	GRCGYC	2 cut(s)	1238, 1691
BsaI	GGTCTC	1 cut(s)	1651
BsaJI	CCNNGG	2 cut(s)	1566, 2362
BsaWI	WCCGGW	2 cut(s)	1164, 2322
Bsc4I	CCNNNNNNNGG	9 cut(s)	70, 93, 425, 650, 913, 1102, 1199, 1346, 1899
Bse118I	RCCGGY	1 cut(s)	2322
Bse1I	ACTGG	3 cut(s)	126, 272, 887
Bse21I	CCTNAGG	1 cut(s)	958
Bse3DI	GCAATG	2 cut(s)	1508, 2397
Bse8I	GATNNNNATC	2 cut(s)	1307, 1743
BseAI	TCCGGA	1 cut(s)	1164
BseBI	CCWGG	1 cut(s)	1643
BseDI	CCNNGG	2 cut(s)	1566, 2362
BseGI	GGATG	7 cut(s)	142, 178, 245, 1804, 2293, 2299, 2446
BseJI	GATNNNNATC	2 cut(s)	1307, 1743
BseLI	CCNNNNNNNGG	9 cut(s)	70, 93, 425, 650, 913, 1102, 1199, 1346, 1899
BseMI	GCAATG	2 cut(s)	1508, 2397
BseMII	CTCAG	5 cut(s)	614, 1032, 1572, 1662, 1878
BseNI	ACTGG	3 cut(s)	126, 272, 887
BseRI	GAGGAG	1 cut(s)	1888
BseXI	GCAGC	9 cut(s)	733, 1368, 1434, 1522, 1564, 2035, 2097, 2173, 2309
BseYI	CCCAGC	2 cut(s)	1918, 2026
Bsh1236I	CGCG	1 cut(s)	556
BshFI	GGCC	8 cut(s)	74, 697, 928, 1192, 1273, 1338, 1641, 1892
BshNI	GGYRCC	1 cut(s)	1562
BshTI	ACCGGT	1 cut(s)	2322
BsiHKAI	GWGCWC	4 cut(s)	1040, 1144, 1945, 2319
BsiHKCI	CYCGRG	1 cut(s)	1454
BsiSI	CCGG	3 cut(s)	392, 1165, 2323
BslFI	GGGAC	2 cut(s)	1093, 1249
BslI	CCNNNNNNNGG	9 cut(s)	70, 93, 425, 650, 913, 1102, 1199, 1346, 1899
BsmAI	GTCTC	1 cut(s)	1651
BsmFI	GGGAC	2 cut(s)	1093, 1249
BsmI	GAATGC	1 cut(s)	395
BsnI	GGCC	8 cut(s)	74, 697, 928, 1192, 1273, 1338, 1641, 1892
Bso31I	GGTCTC	1 cut(s)	1651
BsoBI	CYCGRG	1 cut(s)	1454
Bsp119I	TTCGAA	1 cut(s)	636
Bsp1286I	GDGCHC	6 cut(s)	65, 1040, 1144, 1945, 2033, 2319
Bsp13I	TCCGGA	1 cut(s)	1164
Bsp143I	GATC	9 cut(s)	89, 402, 1651, 1744, 1802, 1923, 1948, 2155, 2414
BspACI	CCGC	5 cut(s)	237, 440, 1151, 1253, 2183
BspANI	GGCC	8 cut(s)	74, 697, 928, 1192, 1273, 1338, 1641, 1892
BspCNI	CTCAG	5 cut(s)	613, 1033, 1573, 1661, 1879
BspEI	TCCGGA	1 cut(s)	1164
BspFNI	CGCG	1 cut(s)	556
BspLI	GGNNCC	5 cut(s)	664, 1081, 1564, 1746, 2032
BspMAI	CTGCAG	1 cut(s)	2163
BspMI	ACCTGC	1 cut(s)	2161
BspOI	GCTAGC	1 cut(s)	417
BspPI	GGATC	7 cut(s)	1646, 1739, 1752, 1810, 1918, 1956, 2422
BspT104I	TTCGAA	1 cut(s)	636
BspT107I	GGYRCC	1 cut(s)	1562
BspTNI	GGTCTC	1 cut(s)	1651
BsrDI	GCAATG	2 cut(s)	1508, 2397
BsrFI	RCCGGY	1 cut(s)	2322
BsrI	ACTGG	3 cut(s)	126, 272, 887
BssAI	RCCGGY	1 cut(s)	2322
BssECI	CCNNGG	2 cut(s)	1566, 2362
BssMI	GATC	9 cut(s)	89, 402, 1651, 1744, 1802, 1923, 1948, 2155, 2414
BssNI	GRCGYC	2 cut(s)	1238, 1691
BssSI	CACGAG	1 cut(s)	1341
Bst2BI	CACGAG	1 cut(s)	1341
Bst2UI	CCWGG	1 cut(s)	1643
Bst4CI	ACNGT	8 cut(s)	379, 1072, 1146, 1330, 2312, 2363, 2384, 2448
Bst6I	CTCTTC	2 cut(s)	1843, 2428
BstACI	GRCGYC	2 cut(s)	1238, 1691
BstAPI	GCANNNNNTGC	3 cut(s)	578, 1991, 2167
BstBI	TTCGAA	1 cut(s)	636
BstC8I	GCNNGC	6 cut(s)	76, 300, 415, 1831, 2115, 2128
BstDSI	CCRYGG	1 cut(s)	2362
BstENI	CCTNNNNNAGG	1 cut(s)	911
BstF5I	GGATG	7 cut(s)	142, 178, 245, 1804, 2293, 2299, 2446
BstFNI	CGCG	1 cut(s)	556
BstHHI	GCGC	1 cut(s)	2009
BstKTI	GATC	9 cut(s)	92, 405, 1654, 1747, 1805, 1926, 1951, 2158, 2417
BstMAI	GTCTC	1 cut(s)	1651
BstMBI	GATC	9 cut(s)	89, 402, 1651, 1744, 1802, 1923, 1948, 2155, 2414
BstNI	CCWGG	1 cut(s)	1643
BstNSI	RCATGY	3 cut(s)	302, 1252, 1913
BstSCI	CCNGG	1 cut(s)	1641
BstSFI	CTRYAG	3 cut(s)	848, 1230, 2159
BstUI	CGCG	1 cut(s)	556
BstV1I	GCAGC	9 cut(s)	733, 1368, 1434, 1522, 1564, 2035, 2097, 2173, 2309
BstV2I	GAAGAC	2 cut(s)	753, 2235
BstX2I	RGATCY	4 cut(s)	1651, 1744, 1948, 2414
BstXI	CCANNNNNNTGG	2 cut(s)	1687, 1945
BstYI	RGATCY	4 cut(s)	1651, 1744, 1948, 2414
Bsu36I	CCTNAGG	1 cut(s)	958
BsuI	GTATCC	2 cut(s)	32, 831
BsuRI	GGCC	8 cut(s)	74, 697, 928, 1192, 1273, 1338, 1641, 1892
BtgI	CCRYGG	1 cut(s)	2362
BtrI	CACGTC	2 cut(s)	1808, 2071
BtsCI	GGATG	7 cut(s)	142, 178, 245, 1804, 2293, 2299, 2446
BtsI	GCAGTG	1 cut(s)	1866
BtsIMutI	CAGTG	7 cut(s)	894, 1117, 1142, 1316, 1335, 1866, 2317
BveI	ACCTGC	1 cut(s)	2161
Cac8I	GCNNGC	6 cut(s)	76, 300, 415, 1831, 2115, 2128
CfoI	GCGC	1 cut(s)	2009
Cfr10I	RCCGGY	1 cut(s)	2322
Cfr13I	GGNCC	3 cut(s)	1080, 1337, 1639
CseI	GACGC	3 cut(s)	545, 1680, 2207
Csp6I	GTAC	4 cut(s)	449, 776, 866, 2204
CspAI	ACCGGT	1 cut(s)	2322
CviAII	CATG	6 cut(s)	299, 1249, 1855, 1910, 2238, 2306
CviQI	GTAC	4 cut(s)	449, 776, 866, 2204
DpnI	GATC	9 cut(s)	91, 404, 1653, 1746, 1804, 1925, 1950, 2157, 2416
DpnII	GATC	9 cut(s)	89, 402, 1651, 1744, 1802, 1923, 1948, 2155, 2414
DraIII	CACNNNGTG	1 cut(s)	2363
DrdI	GACNNNNNNGTC	1 cut(s)	860
DseDI	GACNNNNNNGTC	1 cut(s)	860
EaeI	YGGCCR	2 cut(s)	926, 1271
Eam1104I	CTCTTC	2 cut(s)	1843, 2428
EarI	CTCTTC	2 cut(s)	1843, 2428
Ecl136II	GAGCTC	3 cut(s)	1038, 1943, 2317
Eco147I	AGGCCT	1 cut(s)	697
Eco24I	GRGCYC	5 cut(s)	65, 1040, 1945, 2033, 2319
Eco31I	GGTCTC	1 cut(s)	1651
Eco47I	GGWCC	1 cut(s)	1080
Eco53kI	GAGCTC	3 cut(s)	1038, 1943, 2317
Eco57I	CTGAAG	3 cut(s)	750, 764, 1991
Eco81I	CCTNAGG	1 cut(s)	958
Eco88I	CYCGRG	1 cut(s)	1454
EcoICRI	GAGCTC	3 cut(s)	1038, 1943, 2317
EcoNI	CCTNNNNNAGG	1 cut(s)	911
EcoO109I	RGGNCCY	2 cut(s)	1080, 1337
EcoRII	CCWGG	1 cut(s)	1641
EcoT38I	GRGCYC	5 cut(s)	65, 1040, 1945, 2033, 2319
FaeI	CATG	6 cut(s)	302, 1252, 1858, 1913, 2241, 2309
FaqI	GGGAC	2 cut(s)	1093, 1249
FatI	CATG	6 cut(s)	298, 1248, 1854, 1909, 2237, 2305
FauI	CCCGC	1 cut(s)	433
FauNDI	CATATG	1 cut(s)	154
FokI	GGATG	6 cut(s)	129, 165, 252, 1811, 2300, 2306
FriOI	GRGCYC	5 cut(s)	65, 1040, 1945, 2033, 2319
FspBI	CTAG	5 cut(s)	221, 414, 833, 908, 1767
GlaI	GCGC	1 cut(s)	2008
GsaI	CCCAGC	2 cut(s)	1922, 2030
GsuI	CTGGAG	1 cut(s)	1158
HaeIII	GGCC	8 cut(s)	74, 697, 928, 1192, 1273, 1338, 1641, 1892
HapII	CCGG	3 cut(s)	392, 1165, 2323
HgaI	GACGC	3 cut(s)	545, 1680, 2207
HhaI	GCGC	1 cut(s)	2009
Hin1I	GRCGYC	2 cut(s)	1238, 1691
Hin1II	CATG	6 cut(s)	302, 1252, 1858, 1913, 2241, 2309
Hin6I	GCGC	1 cut(s)	2007
HinP1I	GCGC	1 cut(s)	2007
HincII	GTYRAC	2 cut(s)	709, 2087
HindII	GTYRAC	2 cut(s)	709, 2087
HindIII	AAGCTT	1 cut(s)	2036
HinfI	GANTC	6 cut(s)	8, 469, 475, 511, 1598, 2099
HpaII	CCGG	3 cut(s)	392, 1165, 2323
HphI	GGTGA	6 cut(s)	25, 812, 1084, 1600, 1768, 2185
Hpy166II	GTNNAC	8 cut(s)	709, 803, 965, 1591, 1607, 1680, 2074, 2087
Hpy188III	TCNNGA	7 cut(s)	87, 400, 1165, 1312, 1946, 2000, 2096
Hpy8I	GTNNAC	8 cut(s)	709, 803, 965, 1591, 1607, 1680, 2074, 2087
HpyAV	CCTTC	7 cut(s)	592, 643, 934, 1007, 1524, 1753, 1758
HpyCH4III	ACNGT	8 cut(s)	379, 1072, 1146, 1330, 2312, 2363, 2384, 2448
HpyCH4IV	ACGT	5 cut(s)	362, 677, 1238, 1807, 2070
HpySE526I	ACGT	5 cut(s)	362, 677, 1238, 1807, 2070
Hsp92I	GRCGYC	2 cut(s)	1238, 1691
Hsp92II	CATG	6 cut(s)	302, 1252, 1858, 1913, 2241, 2309
HspAI	GCGC	1 cut(s)	2007
Kpn2I	TCCGGA	1 cut(s)	1164
Kzo9I	GATC	9 cut(s)	89, 402, 1651, 1744, 1802, 1923, 1948, 2155, 2414
LmnI	GCTCC	9 cut(s)	196, 541, 1035, 1124, 1139, 2036, 2107, 2123, 2322
Lsp1109I	GCAGC	9 cut(s)	733, 1368, 1434, 1522, 1564, 2035, 2097, 2173, 2309
LweI	GCATC	7 cut(s)	151, 187, 1772, 1842, 1981, 2248, 2284
MaeI	CTAG	5 cut(s)	221, 414, 833, 908, 1767
MaeII	ACGT	5 cut(s)	362, 677, 1238, 1807, 2070
MaeIII	GTNAC	5 cut(s)	13, 128, 373, 1225, 2428
MalI	GATC	9 cut(s)	91, 404, 1653, 1746, 1804, 1925, 1950, 2157, 2416
MboI	GATC	9 cut(s)	89, 402, 1651, 1744, 1802, 1923, 1948, 2155, 2414
MfeI	CAATTG	1 cut(s)	1656
MflI	RGATCY	4 cut(s)	1651, 1744, 1948, 2414
MhlI	GDGCHC	6 cut(s)	65, 1040, 1144, 1945, 2033, 2319
MlsI	TGGCCA	1 cut(s)	928
MluNI	TGGCCA	1 cut(s)	928
MlyI	GAGTC	1 cut(s)	17
MmeI	TCCRAC	1 cut(s)	1327
Mox20I	TGGCCA	1 cut(s)	928
MroI	TCCGGA	1 cut(s)	1164
MscI	TGGCCA	1 cut(s)	928
MseI	TTAA	9 cut(s)	336, 408, 596, 687, 1461, 1515, 2151, 2189, 2246
MslI	CAYNNNNRTG	2 cut(s)	1247, 2310
Msp20I	TGGCCA	1 cut(s)	928
MspA1I	CMGCKG	1 cut(s)	2026
MspI	CCGG	3 cut(s)	392, 1165, 2323
MspR9I	CCNGG	1 cut(s)	1643
MunI	CAATTG	1 cut(s)	1656
Mva1269I	GAATGC	1 cut(s)	395
MvaI	CCWGG	1 cut(s)	1643
MvnI	CGCG	1 cut(s)	556
NdeI	CATATG	1 cut(s)	154
NdeII	GATC	9 cut(s)	89, 402, 1651, 1744, 1802, 1923, 1948, 2155, 2414
NheI	GCTAGC	1 cut(s)	413
NlaIII	CATG	6 cut(s)	302, 1252, 1858, 1913, 2241, 2309
NlaIV	GGNNCC	5 cut(s)	664, 1081, 1564, 1746, 2032
NmeAIII	GCCGAG	1 cut(s)	1591
NmuCI	GTSAC	1 cut(s)	13
NspI	RCATGY	3 cut(s)	302, 1252, 1913
NspV	TTCGAA	1 cut(s)	636
PaeI	GCATGC	1 cut(s)	302
PaeR7I	CTCGAG	1 cut(s)	1454
PaqCI	CACCTGC	1 cut(s)	2161
PceI	AGGCCT	1 cut(s)	697
PctI	GAATGC	1 cut(s)	395
PfeI	GAWTC	5 cut(s)	469, 475, 511, 1598, 2099
PflMI	CCANNNNNTGG	2 cut(s)	650, 1102
PinAI	ACCGGT	1 cut(s)	2322
PleI	GAGTC	1 cut(s)	16
PpsI	GAGTC	1 cut(s)	16
PpuMI	RGGWCCY	1 cut(s)	1080
PshBI	ATTAAT	1 cut(s)	336
PsiI	TTATAA	1 cut(s)	1475
Psp124BI	GAGCTC	3 cut(s)	1040, 1945, 2319
Psp5II	RGGWCCY	1 cut(s)	1080
Psp6I	CCWGG	1 cut(s)	1641
PspFI	CCCAGC	2 cut(s)	1918, 2026
PspGI	CCWGG	1 cut(s)	1641
PspN4I	GGNNCC	5 cut(s)	664, 1081, 1564, 1746, 2032
PspPI	GGNCC	3 cut(s)	1080, 1337, 1639
PspPPI	RGGWCCY	1 cut(s)	1080
PspXI	VCTCGAGB	1 cut(s)	1454
PstI	CTGCAG	1 cut(s)	2163
PsuI	RGATCY	4 cut(s)	1651, 1744, 1948, 2414
PvuII	CAGCTG	1 cut(s)	2026
RsaI	GTAC	4 cut(s)	450, 777, 867, 2205
RsaNI	GTAC	4 cut(s)	449, 776, 866, 2204
RseI	CAYNNNNRTG	2 cut(s)	1247, 2310
SacI	GAGCTC	3 cut(s)	1040, 1945, 2319
SaqAI	TTAA	9 cut(s)	336, 408, 596, 687, 1461, 1515, 2151, 2189, 2246
Sau3AI	GATC	9 cut(s)	89, 402, 1651, 1744, 1802, 1923, 1948, 2155, 2414
Sau96I	GGNCC	3 cut(s)	1080, 1337, 1639
SchI	GAGTC	1 cut(s)	17
ScrFI	CCNGG	1 cut(s)	1643
SduI	GDGCHC	6 cut(s)	65, 1040, 1144, 1945, 2033, 2319
SfaNI	GCATC	7 cut(s)	151, 187, 1772, 1842, 1981, 2248, 2284
SfcI	CTRYAG	3 cut(s)	848, 1230, 2159
Sfr274I	CTCGAG	1 cut(s)	1454
SfuI	TTCGAA	1 cut(s)	636
SinI	GGWCC	1 cut(s)	1080
SlaI	CTCGAG	1 cut(s)	1454
SmiMI	CAYNNNNRTG	2 cut(s)	1247, 2310
SmlI	CTYRAG	5 cut(s)	691, 1392, 1454, 1952, 2118
SmoI	CTYRAG	5 cut(s)	691, 1392, 1454, 1952, 2118
SphI	GCATGC	1 cut(s)	302
SseBI	AGGCCT	1 cut(s)	697
SsiI	CCGC	5 cut(s)	237, 440, 1151, 1253, 2183
SspMI	CTAG	5 cut(s)	221, 414, 833, 908, 1767
SstI	GAGCTC	3 cut(s)	1040, 1945, 2319
StuI	AGGCCT	1 cut(s)	697
StyD4I	CCNGG	1 cut(s)	1641
TaaI	ACNGT	8 cut(s)	379, 1072, 1146, 1330, 2312, 2363, 2384, 2448
TaiI	ACGT	5 cut(s)	365, 680, 1241, 1810, 2073
TaqI	TCGA	4 cut(s)	636, 1198, 1455, 1906
TatI	WGTACW	2 cut(s)	448, 775
TauI	GCSGC	1 cut(s)	1255
TfiI	GAWTC	5 cut(s)	469, 475, 511, 1598, 2099
Tru1I	TTAA	9 cut(s)	336, 408, 596, 687, 1461, 1515, 2151, 2189, 2246
Tru9I	TTAA	9 cut(s)	336, 408, 596, 687, 1461, 1515, 2151, 2189, 2246
TscAI	CASTG	7 cut(s)	894, 1117, 1149, 1323, 1335, 1873, 2317
TseFI	GTSAC	1 cut(s)	13
TseI	GCWGC	9 cut(s)	746, 1381, 1447, 1535, 1577, 2023, 2110, 2161, 2297
Tsp45I	GTSAC	1 cut(s)	13
TspDTI	ATGAA	4 cut(s)	537, 741, 1533, 2408
TspGWI	ACGGA	2 cut(s)	108, 2416
TspRI	CASTG	7 cut(s)	894, 1117, 1149, 1323, 1335, 1873, 2317
Van91I	CCANNNNNTGG	2 cut(s)	650, 1102
VpaK11BI	GGWCC	1 cut(s)	1080
VspI	ATTAAT	1 cut(s)	336
XagI	CCTNNNNNAGG	1 cut(s)	911
XapI	RAATTY	4 cut(s)	669, 992, 1159, 1556
XceI	RCATGY	3 cut(s)	302, 1252, 1913
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1852
XhoI	CTCGAG	1 cut(s)	1454
XspI	CTAG	5 cut(s)	221, 414, 833, 908, 1767
ZraI	GACGTC	1 cut(s)	1239

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.