Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003895

Physical Location & Seq

Genomic Coordinates Forward (+)

7866308 .. 7877339

Gene Structure 11032 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1058200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2367 bp

ATGGCCTGCTCTCTGTCCATATCACGGTTTTCTGGGTGTTCTTTTATTCCGTATTCCAGTAACCAGCAAGTGCATCCGTTATCATATGCCACTAATTACAAGACAAGGCATCCCCCTTTTGTTGCAAGAAACTTCTTGGGAAATCCTTTACTAGCTTCCTCTGTATGCGGATGGAGAGGACTTTATTTCTCAAACCACCAACCAGTCCTTTCAAAAAGATTGCGGATACATGCAGGACTTGATGTTGCCAGCGCTGTTGATGTCATTAGTGATTTGGGATTTGATACTTTGACGTTCTTAGCTGTTACTGTCATTATCGTTCCAGCATTCAAGATCATTAAAGCTAGTCCTATACTTGGTTTCTTCTTTGCGGGGATTACACTCAATCAATTTGGCTTGATTCGGAATCTTACAGATGTGAAAGTGTTGTCTGAATGGGGCATTCTTTTCTTGCTGTTTGAGATGGGCTTGGAGCTTTCATTTTCACGTCTGAAGGCTCTTGCAAAATTTGCCTTCGGAATGGGATTAACTCAGGTCGTATTATCTACTCTTGCTTTCACAGCCTTCGAACTTCCACCTAATGGGGCTATTGGAACCCAAATTTTGACGTTCCTCTTTAACTCAAGGCCTGATTTGGTCAACATTAGAAGCGTTGATGAAGCCGTAGTGATTGGTGCTGCTCTCTCTCTGTCTTCTTCAGCTTTTGTACTTCAGCTACTTGCAGAGAAAGGTGAACTCCCTACAAGATTTGGGTCAGCAACTTTAGGGATACTTCTTTTACAGGACATAGCAGTCGTACCTCTCTTAGTCATACTTCCAGTGCTGGAAAGCCAGAACCTAGCAGAGGAAAGTATATGGCCAATGCTTCTGAAGGAAAGTCTAAAAGCCTTAGGTGGACTTGGTATTCTTTCTCTTGGAGGAAAATTCCTTCTTAGACGAGTTTTTGAGTTTGTAGCAGAAGCAAGGAGCTCTGAGGCTTTTGTTGCACTTTGCTTATTGACAGTTGCAGGGACCTCACTTCTCACCCAGAAGTTGGGTTTCAGTGACACGCTTGGAGCATTTTTGGCTGGAGCACTGTTAGCGGAAACAAATTTCCGGACACAAATTGAAGCTGATATAAGGCCATTTCGAGGCCTACTTCTTGGATTATTTTTTGTAACTACTGGGACGTCCATTGACACGCAGCTTCTTTTCCGAGAGTGGCCGAATGTGCTTTCTCTCTTGGCAGGTTTGATTGTCATCAAGACACTGATAATAACAGCAATAGGCCCTCGTGTTGGACTTACTTTACAAGAAAGTGTAAGAATAGGACTGCTTCTATCTCAAGGAGGCGAGTTCGGATTTGTAGTTTTTTCACTTGCAAATAGGCTTGGTGTACTGCCACTCGAGCTTAACAAGTTGCTTATAATTGTTGTTGTCTTGTCAATGGCATTGACCCCATTGCTTAATGATGCTGGAAGAAGGGCTGCTGAATTGATTGATGACAAATTTGGTGCCGAGGATAAAACTGCTGAGGTGGTGAACTTTGATTCAAGTGAACCTGTTGTTATTCTTGGATTTGGACAAATGGGCCAGGTCCTTGCCAATTTCTTGTCCACCCCATTGGCTTCCGGGATAAATAGCGATAATCTGGGATGGCCGTTTGTAGCTTTTGATCTGGATCCTTCTGTGGTGAAGGCGTCTAGGGAACTTGGTTTTCCAATTCTGTACGGGGATGGATCACGTCCAGCAGTTTTGCAATCTGCTGGCATCGCTTCCCCAAAAGCTGTTATGGTTATGTACACTGCAAGGAGTAAAACTACTGAGGCCGTTCAAAGGCTTCGACAAGCTTTCCCAGCGATCCCAATTTATGCCAGAGCTCTGGATCTCAAGCACCTTTTAGAACTGAAGCAATCAGGTGCAACAGATGCTATTCTGGAAAGCGCAGAGACGAGTTTGCAGCTGGGCTCCAAGCTTTTGAAAGGGTTTGGAGTTATGTCCGATGATGTGAACTTTCTTCGTCAACTCATTCGGGATTCCATGGAGCTCCAAGCTCAAGGAGTGAGCAAAACTGATGATAAAGAACTTAATGATTTGCAACCGATGCAGGTGAGAGTTGCGGACTTAATTGATGACGCTGTACCCCTTTCACCAACTCCATCAGAAGACAAATCATGGGGGGTAAACCAGGAAGATGCTTCTTATAGCGTGGCGTTTGAGGGGGATGTGGATGAAGCAAAGCATGACAGTGAGCTCCACCGGTCAGGGCATACAGAAGAAAAAGAAGAAGTTTCACATCGCGGCTTAGATACAGAGAACGGTATTGCAATGAAATCACAAGACGTTGAGGGATCAACCTCTTGCGTAACAACAAAGGATGAACTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006996 GO:0008150 GO:0008324 GO:0009314 GO:0009416 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009628 GO:0009642 GO:0009643 GO:0009657 GO:0009658 GO:0009893 GO:0009987 GO:0010109 GO:0015075 GO:0015318 GO:0016020 GO:0016043 GO:0019222 GO:0022857 GO:0022890 GO:0031323 GO:0031325 GO:0031976 GO:0031984 GO:0034220 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0065007 GO:0071840 GO:0098655 GO:1905157

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger TrkA_N

Protein Analysis

789

Amino Acids

85.13

Weight (kDa)

5.14

Isoelectric Point (pI)

35.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	102 - 483	7.7e-67	Sodium/hydrogen exchanger, transmembrane
TrkA_N	PF02254	515 - 638	1.4e-16	TrkA-N domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003895)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01790 AT1G01790 AT4G04850 AT4G04850
fragaria_vesca	FvH4_2g11000
malus_domestica	MD05G1050600.v1.1 MD10G1058200.v1.1
prunus_persica	Prupe.8G077000_v2.0.a1 Prupe.8G077000_v2.0.a1
pyrus_communis	pycom05g04260 pycom05g04270 pycom10g04490
rosa_chinensis	RchiOBHm_Chr6g0269141 RchiOBHm_Chr6g0269211 RchiOBHm_Chr7g0201951
rosa_laevigata	RLG00000017131
rosa_multiflora	Rmu_co8385553.1_g000001 Rmu_sc0005353.1_g000001
rosa_roxburghii	Rroxscaffold_3G00238290 Rroxscaffold_7G00198940
rosa_rugosa	Rorug05G0148000 Rorug05G0148100 Rorug06G0046600 Rorug06G0046600
rosa_samantha	Rh4DG004100 Rh6AG166300 Rh6BG171900 Rh6CG164800 Rh6CG165400 Rh6DG156300 Rh6DG156400 Rh6DG157000 Rh7AG286500
rosa_wichuraiana	Rw6G014360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1406
AarI	CACCTGC	1 cut(s)	2077
AasI	GACNNNNNNGTC	1 cut(s)	791
AatII	GACGTC	1 cut(s)	1172
Acc36I	ACCTGC	2 cut(s)	1217, 2077
AccB1I	GGYRCC	1 cut(s)	1493
AccB7I	CCANNNNNTGG	2 cut(s)	581, 1033
AccII	CGCG	1 cut(s)	2280
AccIII	TCCGGA	1 cut(s)	1095
AciI	CCGC	6 cut(s)	168, 223, 371, 1082, 2099, 2280
AclWI	GGATC	6 cut(s)	1655, 1668, 1726, 1834, 1872, 2338
AcoI	YGGCCR	3 cut(s)	857, 1202, 1637
AcsI	RAATTY	5 cut(s)	506, 600, 923, 1090, 1487
AcuI	CTGAAG	5 cut(s)	512, 681, 695, 890, 1907
AcyI	GRCGYC	2 cut(s)	1169, 1679
AfaI	GTAC	6 cut(s)	708, 798, 1377, 1709, 1781, 2121
AfeI	AGCGCT	1 cut(s)	253
AfiI	CCNNNNNNNGG	8 cut(s)	24, 356, 581, 844, 1033, 1130, 1277, 1815
AgeI	ACCGGT	1 cut(s)	2238
AgsI	TTSAA	6 cut(s)	213, 331, 1109, 1533, 1814, 1960
AjiI	CACGTC	2 cut(s)	488, 1724
AjnI	CCWGG	2 cut(s)	1572, 2166
Alw21I	GWGCWC	5 cut(s)	971, 1075, 1861, 2028, 2235
Alw26I	GTCTC	1 cut(s)	1922
AlwI	GGATC	6 cut(s)	1655, 1668, 1726, 1834, 1872, 2338
Ama87I	CYCGRG	1 cut(s)	1385
Aor13HI	TCCGGA	1 cut(s)	1095
Aor51HI	AGCGCT	1 cut(s)	253
ApeKI	GCWGC	4 cut(s)	677, 1183, 1466, 1939
ApoI	RAATTY	5 cut(s)	506, 600, 923, 1090, 1487
AsiGI	ACCGGT	1 cut(s)	2238
AspLEI	GCGC	2 cut(s)	254, 1925
AspS9I	GGNCC	4 cut(s)	1011, 1268, 1570, 1576
AsuC2I	CCSGG	1 cut(s)	1612
AsuHPI	GGTGA	6 cut(s)	743, 1015, 1531, 1684, 2101, 2121
AsuII	TTCGAA	1 cut(s)	567
AvaI	CYCGRG	1 cut(s)	1385
AvaII	GGWCC	2 cut(s)	1011, 1576
AxyI	CCTNAGG	1 cut(s)	889
BalI	TGGCCA	1 cut(s)	859
BamHI	GGATCC	1 cut(s)	1660
BanI	GGYRCC	1 cut(s)	1493
BanII	GRGCYC	5 cut(s)	971, 1861, 1949, 2028, 2235
BauI	CACGAG	1 cut(s)	1272
BbsI	GAAGAC	2 cut(s)	684, 2151
Bbv12I	GWGCWC	5 cut(s)	971, 1075, 1861, 2028, 2235
BbvCI	CCTCAGC	1 cut(s)	1512
BbvI	GCAGC	4 cut(s)	664, 1195, 1453, 1951
BccI	CCATC	5 cut(s)	165, 457, 1629, 1709, 2146
BceAI	ACGGC	3 cut(s)	647, 1624, 1793
BciT130I	CCWGG	2 cut(s)	1574, 2168
BciVI	GTATCC	2 cut(s)	219, 762
BcnI	CCSGG	1 cut(s)	1612
BcoDI	GTCTC	1 cut(s)	1922
BfaI	CTAG	4 cut(s)	152, 345, 839, 1683
BfoI	RGCGCY	1 cut(s)	255
BfuAI	ACCTGC	2 cut(s)	1217, 2077
BfuI	GTATCC	2 cut(s)	219, 762
BisI	GCNGC	5 cut(s)	678, 1184, 1467, 1940, 2281
BlsI	GCNGC	5 cut(s)	679, 1185, 1468, 1941, 2282
Bme1390I	CCNGG	3 cut(s)	1574, 1612, 2168
Bme18I	GGWCC	2 cut(s)	1011, 1576
BmeT110I	CYCGRG	1 cut(s)	1385
BmgBI	CACGTC	2 cut(s)	488, 1724
BmgT120I	GGNCC	4 cut(s)	1011, 1268, 1570, 1576
BmiI	GGNNCC	5 cut(s)	595, 1012, 1495, 1662, 1948
BmrFI	CCNGG	3 cut(s)	1574, 1612, 2168
BmrI	ACTGGG	1 cut(s)	1173
BmsI	GCATC	7 cut(s)	82, 118, 1441, 1758, 1897, 2073, 2164
BmuI	ACTGGG	1 cut(s)	1173
BpiI	GAAGAC	2 cut(s)	684, 2151
BpmI	CTGGAG	1 cut(s)	1089
Bpu10I	CCTNAGC	1 cut(s)	1512
Bpu14I	TTCGAA	1 cut(s)	567
BpuEI	CTTGAG	4 cut(s)	607, 1308, 1853, 2019
BpuMI	CCSGG	1 cut(s)	1612
BsaBI	GATNNNNATC	2 cut(s)	1238, 1659
BsaHI	GRCGYC	2 cut(s)	1169, 1679
BsaJI	CCNNGG	2 cut(s)	1497, 2019
BsaWI	WCCGGW	2 cut(s)	1095, 2238
Bsc4I	CCNNNNNNNGG	8 cut(s)	24, 356, 581, 844, 1033, 1130, 1277, 1815
Bse118I	RCCGGY	1 cut(s)	2238
Bse1I	ACTGG	4 cut(s)	57, 203, 818, 1168
Bse21I	CCTNAGG	1 cut(s)	889
Bse3DI	GCAATG	2 cut(s)	1439, 2313
Bse8I	GATNNNNATC	2 cut(s)	1238, 1659
BseAI	TCCGGA	1 cut(s)	1095
BseBI	CCWGG	2 cut(s)	1574, 2168
BseDI	CCNNGG	2 cut(s)	1497, 2019
BseGI	GGATG	8 cut(s)	73, 109, 176, 1640, 1720, 2209, 2215, 2362
BseJI	GATNNNNATC	2 cut(s)	1238, 1659
BseLI	CCNNNNNNNGG	8 cut(s)	24, 356, 581, 844, 1033, 1130, 1277, 1815
BseMI	GCAATG	2 cut(s)	1439, 2313
BseMII	CTCAG	4 cut(s)	545, 963, 1503, 1794
BseNI	ACTGG	4 cut(s)	57, 203, 818, 1168
BseXI	GCAGC	4 cut(s)	664, 1195, 1453, 1951
BseYI	CCCAGC	2 cut(s)	1834, 1942
Bsh1236I	CGCG	1 cut(s)	2280
BshNI	GGYRCC	1 cut(s)	1493
BshTI	ACCGGT	1 cut(s)	2238
BsiHKAI	GWGCWC	5 cut(s)	971, 1075, 1861, 2028, 2235
BsiHKCI	CYCGRG	1 cut(s)	1385
BsiSI	CCGG	3 cut(s)	1096, 1611, 2239
BslFI	GGGAC	2 cut(s)	1024, 1180
BslI	CCNNNNNNNGG	8 cut(s)	24, 356, 581, 844, 1033, 1130, 1277, 1815
BsmAI	GTCTC	1 cut(s)	1922
BsmBI	CGTCTC	1 cut(s)	1922
BsmFI	GGGAC	2 cut(s)	1024, 1180
BsmI	GAATGC	2 cut(s)	326, 441
BsoBI	CYCGRG	1 cut(s)	1385
Bsp119I	TTCGAA	1 cut(s)	567
Bsp1286I	GDGCHC	6 cut(s)	971, 1075, 1861, 1949, 2028, 2235
Bsp13I	TCCGGA	1 cut(s)	1095
Bsp1407I	TGTACA	1 cut(s)	1779
Bsp143I	GATC	7 cut(s)	333, 1654, 1660, 1718, 1839, 1864, 2330
Bsp19I	CCATGG	1 cut(s)	2019
BspACI	CCGC	6 cut(s)	168, 223, 371, 1082, 2099, 2280
BspCNI	CTCAG	4 cut(s)	544, 964, 1504, 1795
BspEI	TCCGGA	1 cut(s)	1095
BspFNI	CGCG	1 cut(s)	2280
BspLI	GGNNCC	5 cut(s)	595, 1012, 1495, 1662, 1948
BspMI	ACCTGC	2 cut(s)	1217, 2077
BspPI	GGATC	6 cut(s)	1655, 1668, 1726, 1834, 1872, 2338
BspT104I	TTCGAA	1 cut(s)	567
BspT107I	GGYRCC	1 cut(s)	1493
BsrDI	GCAATG	2 cut(s)	1439, 2313
BsrFI	RCCGGY	1 cut(s)	2238
BsrGI	TGTACA	1 cut(s)	1779
BsrI	ACTGG	4 cut(s)	57, 203, 818, 1168
BssAI	RCCGGY	1 cut(s)	2238
BssECI	CCNNGG	2 cut(s)	1497, 2019
BssMI	GATC	7 cut(s)	333, 1654, 1660, 1718, 1839, 1864, 2330
BssNI	GRCGYC	2 cut(s)	1169, 1679
BssSI	CACGAG	1 cut(s)	1272
BssT1I	CCWWGG	1 cut(s)	2019
Bst2BI	CACGAG	1 cut(s)	1272
Bst2UI	CCWGG	2 cut(s)	1574, 2168
Bst4CI	ACNGT	7 cut(s)	27, 310, 1003, 1077, 2228, 2300, 2364
BstACI	GRCGYC	2 cut(s)	1169, 1679
BstAPI	GCANNNNNTGC	3 cut(s)	509, 1907, 2083
BstAUI	TGTACA	1 cut(s)	1779
BstBI	TTCGAA	1 cut(s)	567
BstC8I	GCNNGC	3 cut(s)	7, 250, 1747
BstDEI	CTNAG	9 cut(s)	298, 531, 805, 889, 932, 972, 1512, 1803, 2284
BstDSI	CCRYGG	1 cut(s)	2019
BstENI	CCTNNNNNAGG	1 cut(s)	842
BstF5I	GGATG	8 cut(s)	73, 109, 176, 1640, 1720, 2209, 2215, 2362
BstFNI	CGCG	1 cut(s)	2280
BstH2I	RGCGCY	1 cut(s)	255
BstHHI	GCGC	2 cut(s)	254, 1925
BstKTI	GATC	7 cut(s)	336, 1657, 1663, 1721, 1842, 1867, 2333
BstMAI	GTCTC	1 cut(s)	1922
BstMBI	GATC	7 cut(s)	333, 1654, 1660, 1718, 1839, 1864, 2330
BstNI	CCWGG	2 cut(s)	1574, 2168
BstNSI	RCATGY	1 cut(s)	233
BstSCI	CCNGG	3 cut(s)	1572, 1610, 2166
BstUI	CGCG	1 cut(s)	2280
BstV1I	GCAGC	4 cut(s)	664, 1195, 1453, 1951
BstV2I	GAAGAC	2 cut(s)	684, 2151
BstX2I	RGATCY	2 cut(s)	1660, 1864
BstXI	CCANNNNNNTGG	2 cut(s)	1603, 1861
BstYI	RGATCY	2 cut(s)	1660, 1864
Bsu36I	CCTNAGG	1 cut(s)	889
BsuI	GTATCC	2 cut(s)	219, 762
BtgI	CCRYGG	1 cut(s)	2019
BtgZI	GCGATG	2 cut(s)	1735, 2261
BtrI	CACGTC	2 cut(s)	488, 1724
BtsCI	GGATG	8 cut(s)	73, 109, 176, 1640, 1720, 2209, 2215, 2362
BtsI	GCAGTG	1 cut(s)	1782
BtsIMutI	CAGTG	6 cut(s)	825, 1048, 1073, 1247, 1782, 2233
BveI	ACCTGC	2 cut(s)	1217, 2077
Cac8I	GCNNGC	3 cut(s)	7, 250, 1747
CfoI	GCGC	2 cut(s)	254, 1925
Cfr10I	RCCGGY	1 cut(s)	2238
Cfr13I	GGNCC	4 cut(s)	1011, 1268, 1570, 1576
CseI	GACGC	2 cut(s)	1668, 2123
Csp6I	GTAC	6 cut(s)	707, 797, 1376, 1708, 1780, 2120
CspAI	ACCGGT	1 cut(s)	2238
CviAII	CATG	4 cut(s)	230, 2020, 2154, 2222
CviQI	GTAC	6 cut(s)	707, 797, 1376, 1708, 1780, 2120
DdeI	CTNAG	9 cut(s)	298, 531, 805, 889, 932, 972, 1512, 1803, 2284
DpnI	GATC	7 cut(s)	335, 1656, 1662, 1720, 1841, 1866, 2332
DpnII	GATC	7 cut(s)	333, 1654, 1660, 1718, 1839, 1864, 2330
DrdI	GACNNNNNNGTC	1 cut(s)	791
DseDI	GACNNNNNNGTC	1 cut(s)	791
EaeI	YGGCCR	3 cut(s)	857, 1202, 1637
Ecl136II	GAGCTC	4 cut(s)	969, 1859, 2026, 2233
Eco130I	CCWWGG	1 cut(s)	2019
Eco147I	AGGCCT	2 cut(s)	628, 1134
Eco24I	GRGCYC	5 cut(s)	971, 1861, 1949, 2028, 2235
Eco47I	GGWCC	2 cut(s)	1011, 1576
Eco47III	AGCGCT	1 cut(s)	253
Eco53kI	GAGCTC	4 cut(s)	969, 1859, 2026, 2233
Eco57I	CTGAAG	5 cut(s)	512, 681, 695, 890, 1907
Eco81I	CCTNAGG	1 cut(s)	889
Eco88I	CYCGRG	1 cut(s)	1385
EcoICRI	GAGCTC	4 cut(s)	969, 1859, 2026, 2233
EcoNI	CCTNNNNNAGG	1 cut(s)	842
EcoO109I	RGGNCCY	3 cut(s)	1011, 1268, 1576
EcoRII	CCWGG	2 cut(s)	1572, 2166
EcoT14I	CCWWGG	1 cut(s)	2019
EcoT38I	GRGCYC	5 cut(s)	971, 1861, 1949, 2028, 2235
ErhI	CCWWGG	1 cut(s)	2019
Esp3I	CGTCTC	1 cut(s)	1922
FaeI	CATG	4 cut(s)	233, 2023, 2157, 2225
FaqI	GGGAC	2 cut(s)	1024, 1180
FatI	CATG	4 cut(s)	229, 2019, 2153, 2221
FauI	CCCGC	1 cut(s)	364
FauNDI	CATATG	1 cut(s)	85
Fnu4HI	GCNGC	5 cut(s)	678, 1184, 1467, 1940, 2281
FokI	GGATG	7 cut(s)	60, 96, 183, 1647, 1727, 2216, 2222
FriOI	GRGCYC	5 cut(s)	971, 1861, 1949, 2028, 2235
Fsp4HI	GCNGC	5 cut(s)	678, 1184, 1467, 1940, 2281
FspBI	CTAG	4 cut(s)	152, 345, 839, 1683
GlaI	GCGC	2 cut(s)	253, 1924
GluI	GCNGC	5 cut(s)	678, 1184, 1467, 1940, 2281
GsaI	CCCAGC	2 cut(s)	1838, 1946
GsuI	CTGGAG	1 cut(s)	1089
HaeII	RGCGCY	1 cut(s)	255
HapII	CCGG	3 cut(s)	1096, 1611, 2239
HgaI	GACGC	2 cut(s)	1668, 2123
HhaI	GCGC	2 cut(s)	254, 1925
Hin1I	GRCGYC	2 cut(s)	1169, 1679
Hin1II	CATG	4 cut(s)	233, 2023, 2157, 2225
Hin6I	GCGC	2 cut(s)	252, 1923
HinP1I	GCGC	2 cut(s)	252, 1923
HincII	GTYRAC	2 cut(s)	640, 2003
HindII	GTYRAC	2 cut(s)	640, 2003
HindIII	AAGCTT	2 cut(s)	1827, 1952
HinfI	GANTC	4 cut(s)	400, 406, 1529, 2015
HpaII	CCGG	3 cut(s)	1096, 1611, 2239
HphI	GGTGA	6 cut(s)	743, 1015, 1531, 1684, 2101, 2121
Hpy188III	TCNNGA	7 cut(s)	331, 1096, 1243, 1658, 1862, 1916, 2012
HpyAV	CCTTC	8 cut(s)	487, 523, 574, 865, 938, 1455, 1669, 1674
HpyCH4III	ACNGT	7 cut(s)	27, 310, 1003, 1077, 2228, 2300, 2364
HpyCH4IV	ACGT	6 cut(s)	293, 487, 608, 1169, 1723, 2322
HpyF3I	CTNAG	9 cut(s)	298, 531, 805, 889, 932, 972, 1512, 1803, 2284
HpySE526I	ACGT	6 cut(s)	293, 487, 608, 1169, 1723, 2322
Hsp92I	GRCGYC	2 cut(s)	1169, 1679
Hsp92II	CATG	4 cut(s)	233, 2023, 2157, 2225
HspAI	GCGC	2 cut(s)	252, 1923
Kpn2I	TCCGGA	1 cut(s)	1095
Kzo9I	GATC	7 cut(s)	333, 1654, 1660, 1718, 1839, 1864, 2330
LmnI	GCTCC	8 cut(s)	472, 966, 1055, 1070, 1952, 2023, 2031, 2238
Lsp1109I	GCAGC	4 cut(s)	664, 1195, 1453, 1951
LweI	GCATC	7 cut(s)	82, 118, 1441, 1758, 1897, 2073, 2164
MaeI	CTAG	4 cut(s)	152, 345, 839, 1683
MaeII	ACGT	6 cut(s)	293, 487, 608, 1169, 1723, 2322
MaeIII	GTNAC	5 cut(s)	59, 304, 1043, 1156, 2344
MalI	GATC	7 cut(s)	335, 1656, 1662, 1720, 1841, 1866, 2332
MboI	GATC	7 cut(s)	333, 1654, 1660, 1718, 1839, 1864, 2330
MboII	GAAGA	9 cut(s)	355, 684, 687, 1470, 1988, 2156, 2183, 2267, 2276
MflI	RGATCY	2 cut(s)	1660, 1864
MhlI	GDGCHC	6 cut(s)	971, 1075, 1861, 1949, 2028, 2235
MlsI	TGGCCA	1 cut(s)	859
MluNI	TGGCCA	1 cut(s)	859
MmeI	TCCRAC	1 cut(s)	1258
Mox20I	TGGCCA	1 cut(s)	859
MroI	TCCGGA	1 cut(s)	1095
MscI	TGGCCA	1 cut(s)	859
MseI	TTAA	7 cut(s)	339, 527, 618, 1392, 1446, 2067, 2105
MslI	CAYNNNNRTG	1 cut(s)	2226
Msp20I	TGGCCA	1 cut(s)	859
MspA1I	CMGCKG	1 cut(s)	1942
MspI	CCGG	3 cut(s)	1096, 1611, 2239
MspR9I	CCNGG	3 cut(s)	1574, 1612, 2168
Mva1269I	GAATGC	2 cut(s)	326, 441
MvaI	CCWGG	2 cut(s)	1574, 2168
MvnI	CGCG	1 cut(s)	2280
NciI	CCSGG	1 cut(s)	1612
NcoI	CCATGG	1 cut(s)	2019
NdeI	CATATG	1 cut(s)	85
NdeII	GATC	7 cut(s)	333, 1654, 1660, 1718, 1839, 1864, 2330
NlaIII	CATG	4 cut(s)	233, 2023, 2157, 2225
NlaIV	GGNNCC	5 cut(s)	595, 1012, 1495, 1662, 1948
NmeAIII	GCCGAG	1 cut(s)	1522
NmuCI	GTSAC	1 cut(s)	1043
NspI	RCATGY	1 cut(s)	233
NspV	TTCGAA	1 cut(s)	567
PaeR7I	CTCGAG	1 cut(s)	1385
PaqCI	CACCTGC	1 cut(s)	2077
PceI	AGGCCT	2 cut(s)	628, 1134
PctI	GAATGC	2 cut(s)	326, 441
PfeI	GAWTC	4 cut(s)	400, 406, 1529, 2015
PflMI	CCANNNNNTGG	2 cut(s)	581, 1033
PfoI	TCCNGGA	1 cut(s)	1610
PinAI	ACCGGT	1 cut(s)	2238
PkrI	GCNGC	5 cut(s)	679, 1185, 1468, 1941, 2282
PpuMI	RGGWCCY	2 cut(s)	1011, 1576
PsiI	TTATAA	1 cut(s)	1406
Psp124BI	GAGCTC	4 cut(s)	971, 1861, 2028, 2235
Psp5II	RGGWCCY	2 cut(s)	1011, 1576
Psp6I	CCWGG	2 cut(s)	1572, 2166
PspFI	CCCAGC	2 cut(s)	1834, 1942
PspGI	CCWGG	2 cut(s)	1572, 2166
PspN4I	GGNNCC	5 cut(s)	595, 1012, 1495, 1662, 1948
PspPI	GGNCC	4 cut(s)	1011, 1268, 1570, 1576
PspPPI	RGGWCCY	2 cut(s)	1011, 1576
PspXI	VCTCGAGB	1 cut(s)	1385
PsuI	RGATCY	2 cut(s)	1660, 1864
PvuII	CAGCTG	1 cut(s)	1942
RsaI	GTAC	6 cut(s)	708, 798, 1377, 1709, 1781, 2121
RsaNI	GTAC	6 cut(s)	707, 797, 1376, 1708, 1780, 2120
RseI	CAYNNNNRTG	1 cut(s)	2226
SacI	GAGCTC	4 cut(s)	971, 1861, 2028, 2235
SaqAI	TTAA	7 cut(s)	339, 527, 618, 1392, 1446, 2067, 2105
SatI	GCNGC	5 cut(s)	678, 1184, 1467, 1940, 2281
Sau3AI	GATC	7 cut(s)	333, 1654, 1660, 1718, 1839, 1864, 2330
Sau96I	GGNCC	4 cut(s)	1011, 1268, 1570, 1576
ScrFI	CCNGG	3 cut(s)	1574, 1612, 2168
SduI	GDGCHC	6 cut(s)	971, 1075, 1861, 1949, 2028, 2235
SfaNI	GCATC	7 cut(s)	82, 118, 1441, 1758, 1897, 2073, 2164
Sfr274I	CTCGAG	1 cut(s)	1385
SfuI	TTCGAA	1 cut(s)	567
SinI	GGWCC	2 cut(s)	1011, 1576
SlaI	CTCGAG	1 cut(s)	1385
SmiMI	CAYNNNNRTG	1 cut(s)	2226
SmlI	CTYRAG	5 cut(s)	622, 1323, 1385, 1868, 2034
SmoI	CTYRAG	5 cut(s)	622, 1323, 1385, 1868, 2034
SseBI	AGGCCT	2 cut(s)	628, 1134
SsiI	CCGC	6 cut(s)	168, 223, 371, 1082, 2099, 2280
SspMI	CTAG	4 cut(s)	152, 345, 839, 1683
SstI	GAGCTC	4 cut(s)	971, 1861, 2028, 2235
StuI	AGGCCT	2 cut(s)	628, 1134
StyD4I	CCNGG	3 cut(s)	1572, 1610, 2166
StyI	CCWWGG	1 cut(s)	2019
TaaI	ACNGT	7 cut(s)	27, 310, 1003, 1077, 2228, 2300, 2364
TaiI	ACGT	6 cut(s)	296, 490, 611, 1172, 1726, 2325
TaqI	TCGA	4 cut(s)	567, 1129, 1386, 1822
TatI	WGTACW	3 cut(s)	706, 1375, 1779
TauI	GCSGC	1 cut(s)	2283
TfiI	GAWTC	4 cut(s)	400, 406, 1529, 2015
Tru1I	TTAA	7 cut(s)	339, 527, 618, 1392, 1446, 2067, 2105
Tru9I	TTAA	7 cut(s)	339, 527, 618, 1392, 1446, 2067, 2105
TscAI	CASTG	6 cut(s)	825, 1048, 1080, 1254, 1789, 2233
TseFI	GTSAC	1 cut(s)	1043
TseI	GCWGC	4 cut(s)	677, 1183, 1466, 1939
Tsp45I	GTSAC	1 cut(s)	1043
TspDTI	ATGAA	4 cut(s)	468, 672, 2226, 2324
TspGWI	ACGGA	2 cut(s)	39, 66
TspRI	CASTG	6 cut(s)	825, 1048, 1080, 1254, 1789, 2233
Van91I	CCANNNNNTGG	2 cut(s)	581, 1033
VpaK11BI	GGWCC	2 cut(s)	1011, 1576
XagI	CCTNNNNNAGG	1 cut(s)	842
XapI	RAATTY	5 cut(s)	506, 600, 923, 1090, 1487
XceI	RCATGY	1 cut(s)	233
XhoI	CTCGAG	1 cut(s)	1385
XspI	CTAG	4 cut(s)	152, 345, 839, 1683
ZraI	GACGTC	1 cut(s)	1170

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.