Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0001013

Physical Location & Seq

Genomic Coordinates Reverse (-)

25015364 .. 25022297

Gene Structure 6934 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1126800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1992 bp

ATGGTTAGAGATCCACCTCGGCATGATTCACACCGCCAATGGTGCGCACTTTCTAATTTCCATTTTGTAGTAATTTATTATTTATTTACTCCTATTATTATTATTATTATTATTTTATTTTTCAGTTGGAACCCTCTGAAATCCTGCGCATATAAACCCAGCTGTGCGTCGTTTCACGTAAAAAGCGAAAAAACCAACAGACTTTCTCTCTCACTTTCTCTCTCCGCCATTTTTTCTTCCTCCGTTTACTTAATTCTGTCGCGTTCGCCGCCAATGGTGATGGAGGACAACAGCGAGAGCTGCGGGAGTAGAGCGCTGAACGACGCGTCGTCTTCTCCGGTTCAGTCTCGCCAGCAGAGAAAGCTCGGGGTTTACAATGAGGTCCTCCGCCGCCTCAAGGACTCCAAAAACGACGAAGCCATTTGCCCTGGCTTCGACGACGAGCTTTGGGCTCACTTCAATCGCCTCCCCACTCGGTATGCATTAGATGTGAATGTGGAGAGGGCAGAAGATGTGCTTATGCACAAGAGATTACTGCATTTGGCTCATGATCCTGCTCATCGACCAGCGATCGAAGTCCGCCTTGTACAGGTTCATTCTGTTGCAGATGGGAATATAGCAGATGCTACAGATTCTCCGGGTAAAGATGCTGCCCAAAGTTCTAACATATCTAGCAGACTAAGTATGCATCCTCCTCCAGCATTCGGCTCATCTCCTAACCTTGAAGCCCTTGCACTTGAGGCAAGTAAATCTGAAGATCATGATGATGAACATTCTGTACATGCCAGAACTCAGTTTACTCGGCCCATATTGCATGAAATTACTTTCTCAACAGACGACAAGCCTAAACTACTCAGTCAGTTGACTTCCTTGCTTGCGGAGATTGGGCTGAACATCCAAGAAGCACATGCTTTTTCCACATTAGATGGTTACTCCTTGGATGTCTTTGTTGTTGATGGCTGGCCTTACGAGGAAACAGAGAAGCTTAAGATCGCAATAAAAAAGGAAGTTTTAAAGATTGAGAGGCCATGGCCACCTACTCATCAATCATCTTCTGGCAGCGAGCATAATCAAGTAGTGATCAAAACTGAACCTGATCATCTGCAAATACCTAATGATGGGACTGATGTATGGGAAATTGATCCTAGACAGTTGAACTTTGGGAACAAAGTTGCATCTGGGTCCTGCGGAGATTTATATAAAGGTACATATTGTACTCAAGAAGTCGCCATTAAAGTCCTCAAGCCTGAGTGTGTAAATTCTGATATGCAGAAAGATTTTGCCCAGGAAGTTTATATTATGAGGAAAGTTCGACACAAGAATGTTGTACAATTCATTGGTGCATGTACCAAGCCTCCAAGCTTGTGCATTGTAACAGAATATATGTCTGGTGGAAGTGTGTATGACTACTTGCATAAACAAAAGGGTGTTTTTAAGCTCCCATCCTTGCTCAAGGTAGCAATCGATGTTTCCAAGGGAATGACCTACTTGCATCAGAATAACATAATCCACAGGGATTTGAAAGCAGCCAATCTCTTGATGGATGAAAATGAAGTCGTTAAGGTTGCTGATTTCGGGGTTGCTAGAGTGATATCTCAATCTGGAGTAATGACAGCAGAAACTGGGACATATAGGTGGATGGCTCCAGAGGTTATAGAACACAAGCCATATGATCACAAGGCTGATGTTTTTAGCTTTGGAGTTGTGTTATGGGAGTTGCTGACCGGAAAGCTTCCATATGAATACTTGACCCCGTTACAAGCAGCCGTTGGAGTTGTTCAAAAGGGCTTACGGCCGACCATCCCAAAGAACACTCCTCCCAAGCTTGCCGAGCTGCTCGAGAAATGCTGGCAACAAGATCCAAAATCAAGACCTGATTTCTTGGAAATCATAGAAATTCTACAGGCATTAGCCAAAGAGGTTGGTGATGGAGAGGAACGACACAAATCATCGGGAGGATTTCTGTCGATCCTGAGACGGGGTCACCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko01521 ko01522 ko04010 ko04012 ko04013 ko04015 ko04024 ko04062 ko04068 ko04150 ko04214 ko04270 ko04320 ko04510 ko04650 ko04720 ko04722 ko04726 ko04730 ko04810 ko04910 ko04914 ko04934 ko05034 ko05160 ko05200 ko05205 ko05210 ko05211 ko05212 ko05213 ko05214 ko05215 ko05216 ko05218 ko05219 ko05220 ko05221 ko05223 ko05224 ko05225 ko05226 map01521 map01522 map04010 map04012 map04013 map04015 map04024 map04062 map04068 map04150 map04214 map04270 map04320 map04510 map04650 map04720 map04722 map04726 map04730 map04810 map04910 map04914 map04934 map05034 map05160 map05200 map05205 map05210 map05211 map05212 map05213 map05214 map05215 map05216 map05218 map05219 map05220 map05221 map05223 map05224 map05225 map05226

Pfam Domains

Protein Families

ACT Pkinase_Tyr

Protein Analysis

664

Amino Acids

74.45

Weight (kDa)

6.41

Isoelectric Point (pI)

52.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	386 - 634	8.4e-76	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	388 - 631	9e-61	Protein kinase domain
Pkinase_fungal	PF17667	481 - 566	7e-06	Fungal protein kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38470 AT4G38470 AT4G38470
fragaria_vesca	FvH4_2g15800 FvH4_2g15800 FvH4_2g15800 FvH4_2g15800 FvH4_2g15800 FvH4_2g15800 FvH4_2g15800 FvH4_2g15800 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880
malus_domestica	MD05G1126800.v1.1 MD10G1130000.v1.1 MD10G1130300.v1.1 MD15G1006000.v1.1
prunus_persica	Prupe.1G358800_v2.0.a1 Prupe.8G170600_v2.0.a1 Prupe.8G170600_v2.0.a1 Prupe.8G170600_v2.0.a1
pyrus_communis	pycom05g12270 pycom15g00460
rosa_chinensis	RchiOBHm_Chr6g0279141 RchiOBHm_Chr6g0303741
rosa_laevigata	RLG00000011050 RLG00000013163
rosa_multiflora	Rmu_co8370501.1_g000001 Rmu_sc0006400.1_g000001 Rmu_sc0009053.1_g000001 Rmu_sc0012878.1_g000007 Rmu_sc0014203.1_g000019
rosa_roxburghii	Rroxscaffold_7G00164440 Rroxscaffold_7G00189090
rosa_rugosa	Rorug06G0122600 Rorug06G0122700 Rorug06G0122800 Rorug06G0122900 Rorug06G0324400
rosa_samantha	Rh6AG231400 Rh6AG438700 Rh6BG236300 Rh6BG464400 Rh6CG238400 Rh6CG451000 Rh6DG229900 Rh6DG437800
rosa_wichuraiana	Rw6G020260 Rw6G037980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	46, 148
AccII	CGCG	2 cut(s)	262, 326
AciI	CCGC	9 cut(s)	34, 225, 269, 303, 388, 391, 580, 878, 1188
AclWI	GGATC	5 cut(s)	5, 545, 1136, 1853, 1963
AcoI	YGGCCR	2 cut(s)	1031, 1793
AcsI	RAATTY	2 cut(s)	1258, 1896
AcuI	CTGAAG	1 cut(s)	774
AfaI	GTAC	6 cut(s)	588, 780, 1207, 1216, 1329, 1348
AfeI	AGCGCT	1 cut(s)	315
AfiI	CCNNNNNNNGG	5 cut(s)	397, 589, 704, 1118, 1978
AflII	CTTAAG	1 cut(s)	986
AflIII	ACRYGT	1 cut(s)	324
AgsI	TTSAA	5 cut(s)	460, 725, 1156, 1522, 1781
AhdI	GACNNNNNGTC	1 cut(s)	328
AjnI	CCWGG	2 cut(s)	427, 1284
Alw26I	GTCTC	2 cut(s)	351, 1969
AlwI	GGATC	5 cut(s)	5, 545, 1136, 1853, 1963
AlwNI	CAGNNNCTG	1 cut(s)	1622
Ama87I	CYCGRG	2 cut(s)	365, 1838
Aor51HI	AGCGCT	1 cut(s)	315
AoxI	GGCC	5 cut(s)	803, 962, 1025, 1031, 1793
ApeKI	GCWGC	6 cut(s)	300, 650, 1059, 1526, 1763, 1834
ApoI	RAATTY	2 cut(s)	1258, 1896
AspLEI	GCGC	3 cut(s)	47, 149, 316
AspS9I	GGNCC	3 cut(s)	382, 804, 1182
AsuC2I	CCSGG	1 cut(s)	639
AsuHPI	GGTGA	3 cut(s)	289, 1937, 1976
AvaI	CYCGRG	2 cut(s)	365, 1838
AvaII	GGWCC	2 cut(s)	382, 1182
BalI	TGGCCA	1 cut(s)	1033
BanII	GRGCYC	1 cut(s)	454
BbsI	GAAGAC	1 cut(s)	324
BbvI	GCAGC	6 cut(s)	287, 637, 1071, 1538, 1775, 1821
BceAI	ACGGC	2 cut(s)	1751, 1808
BciT130I	CCWGG	2 cut(s)	429, 1286
BclI	TGATCA	3 cut(s)	1080, 1096, 1672
BcnI	CCSGG	1 cut(s)	639
BcoDI	GTCTC	2 cut(s)	351, 1969
BfaI	CTAG	3 cut(s)	672, 1146, 1584
BfmI	CTRYAG	2 cut(s)	627, 1901
BfoI	RGCGCY	1 cut(s)	317
BfrI	CTTAAG	1 cut(s)	986
BisI	GCNGC	8 cut(s)	269, 301, 391, 651, 1060, 1527, 1764, 1835
BlsI	GCNGC	8 cut(s)	270, 302, 392, 652, 1061, 1528, 1765, 1836
Bme1390I	CCNGG	3 cut(s)	429, 639, 1286
Bme18I	GGWCC	2 cut(s)	382, 1182
BmeRI	GACNNNNNGTC	1 cut(s)	328
BmeT110I	CYCGRG	2 cut(s)	365, 1838
BmgT120I	GGNCC	3 cut(s)	382, 804, 1182
BmiI	GGNNCC	3 cut(s)	131, 1183, 1644
BmrFI	CCNGG	3 cut(s)	429, 639, 1286
BmrI	ACTGGG	1 cut(s)	1632
BmsI	GCATC	5 cut(s)	613, 637, 697, 1184, 1501
BmuI	ACTGGG	1 cut(s)	1632
BpiI	GAAGAC	1 cut(s)	324
BpmI	CTGGAG	3 cut(s)	681, 1623, 1629
BpuEI	CTTGAG	5 cut(s)	380, 758, 1203, 1226, 1436
BpuMI	CCSGG	1 cut(s)	639
Bsa29I	ATCGAT	1 cut(s)	1464
BsaAI	YACGTR	1 cut(s)	178
BsaJI	CCNNGG	6 cut(s)	17, 427, 936, 1028, 1284, 1473
BsaWI	WCCGGW	2 cut(s)	337, 1724
BsaXI	ACNNNNNCTCC	4 cut(s)	619, 649, 1339, 1369
Bsc4I	CCNNNNNNNGG	5 cut(s)	397, 589, 704, 1118, 1978
Bse1I	ACTGG	1 cut(s)	1627
BseBI	CCWGG	2 cut(s)	429, 1286
BseCI	ATCGAT	1 cut(s)	1464
BseDI	CCNNGG	6 cut(s)	17, 427, 936, 1028, 1284, 1473
BseGI	GGATG	7 cut(s)	688, 894, 946, 1442, 1549, 1644, 1800
BseLI	CCNNNNNNNGG	5 cut(s)	397, 589, 704, 1118, 1978
BseMII	CTCAG	4 cut(s)	806, 868, 1239, 1964
BseNI	ACTGG	1 cut(s)	1627
BseRI	GAGGAG	2 cut(s)	684, 1806
BseX3I	CGGCCG	1 cut(s)	1793
BseXI	GCAGC	6 cut(s)	287, 637, 1071, 1538, 1775, 1821
BseYI	CCCAGC	1 cut(s)	158
Bsh1236I	CGCG	2 cut(s)	262, 326
Bsh1285I	CGRYCG	2 cut(s)	573, 1796
BshFI	GGCC	5 cut(s)	805, 964, 1027, 1033, 1795
BshVI	ATCGAT	1 cut(s)	1464
BsiEI	CGRYCG	2 cut(s)	573, 1796
BsiHKCI	CYCGRG	2 cut(s)	365, 1838
BsiSI	CCGG	3 cut(s)	338, 638, 1725
BslFI	GGGAC	2 cut(s)	1135, 1639
BslI	CCNNNNNNNGG	5 cut(s)	397, 589, 704, 1118, 1978
BsmAI	GTCTC	2 cut(s)	351, 1969
BsmBI	CGTCTC	1 cut(s)	1969
BsmFI	GGGAC	2 cut(s)	1135, 1639
BsmI	GAATGC	1 cut(s)	701
BsnI	GGCC	5 cut(s)	805, 964, 1027, 1033, 1795
BsoBI	CYCGRG	2 cut(s)	365, 1838
Bsp1286I	GDGCHC	1 cut(s)	454
Bsp1407I	TGTACA	3 cut(s)	586, 778, 1327
Bsp19I	CCATGG	1 cut(s)	1028
BspACI	CCGC	9 cut(s)	34, 225, 269, 303, 388, 391, 580, 878, 1188
BspANI	GGCC	5 cut(s)	805, 964, 1027, 1033, 1795
BspCNI	CTCAG	4 cut(s)	805, 867, 1240, 1965
BspDI	ATCGAT	1 cut(s)	1464
BspFNI	CGCG	2 cut(s)	262, 326
BspHI	TCATGA	2 cut(s)	547, 760
BspLI	GGNNCC	3 cut(s)	131, 1183, 1644
BspPI	GGATC	5 cut(s)	5, 545, 1136, 1853, 1963
BspTI	CTTAAG	1 cut(s)	986
BsrGI	TGTACA	3 cut(s)	586, 778, 1327
BsrI	ACTGG	1 cut(s)	1627
BssECI	CCNNGG	6 cut(s)	17, 427, 936, 1028, 1284, 1473
BssT1I	CCWWGG	3 cut(s)	936, 1028, 1473
Bst2UI	CCWGG	2 cut(s)	429, 1286
Bst4CI	ACNGT	1 cut(s)	1152
BstAFI	CTTAAG	1 cut(s)	986
BstAUI	TGTACA	3 cut(s)	586, 778, 1327
BstBAI	YACGTR	1 cut(s)	178
BstC8I	GCNNGC	6 cut(s)	353, 876, 962, 1064, 1827, 1850
BstDEI	CTNAG	5 cut(s)	680, 792, 854, 1248, 1973
BstDSI	CCRYGG	1 cut(s)	1028
BstEII	GGTNACC	1 cut(s)	1982
BstENI	CCTNNNNNAGG	1 cut(s)	587
BstF5I	GGATG	7 cut(s)	688, 894, 946, 1442, 1549, 1644, 1800
BstFNI	CGCG	2 cut(s)	262, 326
BstH2I	RGCGCY	1 cut(s)	317
BstHHI	GCGC	3 cut(s)	47, 149, 316
BstMAI	GTCTC	2 cut(s)	351, 1969
BstMCI	CGRYCG	2 cut(s)	573, 1796
BstMWI	GCNNNNNNNGC	7 cut(s)	42, 268, 300, 361, 740, 811, 1831
BstNI	CCWGG	2 cut(s)	429, 1286
BstNSI	RCATGY	3 cut(s)	785, 911, 1347
BstPI	GGTNACC	1 cut(s)	1982
BstSCI	CCNGG	3 cut(s)	427, 637, 1284
BstSFI	CTRYAG	2 cut(s)	627, 1901
BstUI	CGCG	2 cut(s)	262, 326
BstV1I	GCAGC	6 cut(s)	287, 637, 1071, 1538, 1775, 1821
BstV2I	GAAGAC	1 cut(s)	324
BstX2I	RGATCY	2 cut(s)	10, 1858
BstYI	RGATCY	2 cut(s)	10, 1858
BstZI	CGGCCG	1 cut(s)	1793
Bsu15I	ATCGAT	1 cut(s)	1464
BsuRI	GGCC	5 cut(s)	805, 964, 1027, 1033, 1795
BsuTUI	ATCGAT	1 cut(s)	1464
BtgI	CCRYGG	1 cut(s)	1028
BtsCI	GGATG	7 cut(s)	688, 894, 946, 1442, 1549, 1644, 1800
Cac8I	GCNNGC	6 cut(s)	353, 876, 962, 1064, 1827, 1850
CaiI	CAGNNNCTG	1 cut(s)	1622
CciI	TCATGA	2 cut(s)	547, 760
CfoI	GCGC	3 cut(s)	47, 149, 316
Cfr13I	GGNCC	3 cut(s)	382, 804, 1182
ClaI	ATCGAT	1 cut(s)	1464
CseI	GACGC	3 cut(s)	156, 315, 332
Csp6I	GTAC	6 cut(s)	587, 779, 1206, 1215, 1328, 1347
CviAII	CATG	8 cut(s)	23, 548, 761, 782, 815, 908, 1029, 1344
CviQI	GTAC	6 cut(s)	587, 779, 1206, 1215, 1328, 1347
DdeI	CTNAG	5 cut(s)	680, 792, 854, 1248, 1973
DraI	TTTAAA	1 cut(s)	1014
DriI	GACNNNNNGTC	1 cut(s)	328
EaeI	YGGCCR	2 cut(s)	1031, 1793
EagI	CGGCCG	1 cut(s)	1793
Eam1105I	GACNNNNNGTC	1 cut(s)	328
EciI	GGCGGA	3 cut(s)	214, 377, 569
EclXI	CGGCCG	1 cut(s)	1793
Eco130I	CCWWGG	3 cut(s)	936, 1028, 1473
Eco24I	GRGCYC	1 cut(s)	454
Eco32I	GATATC	1 cut(s)	1593
Eco47I	GGWCC	2 cut(s)	382, 1182
Eco47III	AGCGCT	1 cut(s)	315
Eco52I	CGGCCG	1 cut(s)	1793
Eco57I	CTGAAG	1 cut(s)	774
Eco88I	CYCGRG	2 cut(s)	365, 1838
Eco91I	GGTNACC	1 cut(s)	1982
EcoNI	CCTNNNNNAGG	1 cut(s)	587
EcoO109I	RGGNCCY	2 cut(s)	382, 1182
EcoO65I	GGTNACC	1 cut(s)	1982
EcoRII	CCWGG	2 cut(s)	427, 1284
EcoRV	GATATC	1 cut(s)	1593
EcoT14I	CCWWGG	3 cut(s)	936, 1028, 1473
EcoT22I	ATGCAT	2 cut(s)	484, 690
EcoT38I	GRGCYC	1 cut(s)	454
ErhI	CCWWGG	3 cut(s)	936, 1028, 1473
Esp3I	CGTCTC	1 cut(s)	1969
FaeI	CATG	8 cut(s)	26, 551, 764, 785, 818, 911, 1032, 1347
FalI	AAGNNNNNCTT	4 cut(s)	501, 533, 567, 599
FaqI	GGGAC	2 cut(s)	1135, 1639
FatI	CATG	8 cut(s)	22, 547, 760, 781, 814, 907, 1028, 1343
FauI	CCCGC	1 cut(s)	296
FauNDI	CATATG	2 cut(s)	1669, 1738
FbaI	TGATCA	3 cut(s)	1080, 1096, 1672
Fnu4HI	GCNGC	8 cut(s)	269, 301, 391, 651, 1060, 1527, 1764, 1835
FokI	GGATG	7 cut(s)	675, 881, 953, 1429, 1556, 1651, 1787
FriOI	GRGCYC	1 cut(s)	454
Fsp4HI	GCNGC	8 cut(s)	269, 301, 391, 651, 1060, 1527, 1764, 1835
FspAI	RTGCGCAY	1 cut(s)	46
FspBI	CTAG	3 cut(s)	672, 1146, 1584
FspI	TGCGCA	2 cut(s)	46, 148
GlaI	GCGC	3 cut(s)	46, 148, 315
GluI	GCNGC	8 cut(s)	269, 301, 391, 651, 1060, 1527, 1764, 1835
GsaI	CCCAGC	1 cut(s)	162
GsuI	CTGGAG	3 cut(s)	681, 1623, 1629
HaeII	RGCGCY	1 cut(s)	317
HaeIII	GGCC	5 cut(s)	805, 964, 1027, 1033, 1795
HapII	CCGG	3 cut(s)	338, 638, 1725
HgaI	GACGC	3 cut(s)	156, 315, 332
HhaI	GCGC	3 cut(s)	47, 149, 316
Hin1II	CATG	8 cut(s)	26, 551, 764, 785, 818, 911, 1032, 1347
Hin6I	GCGC	3 cut(s)	45, 147, 314
HinP1I	GCGC	3 cut(s)	45, 147, 314
HincII	GTYRAC	1 cut(s)	864
HindII	GTYRAC	1 cut(s)	864
HindIII	AAGCTT	4 cut(s)	983, 1360, 1730, 1823
HinfI	GANTC	3 cut(s)	26, 401, 632
HpaII	CCGG	3 cut(s)	338, 638, 1725
HphI	GGTGA	3 cut(s)	289, 1937, 1976
Hpy166II	GTNNAC	4 cut(s)	247, 373, 798, 864
Hpy188I	TCNGA	4 cut(s)	138, 754, 1264, 1497
Hpy8I	GTNNAC	4 cut(s)	247, 373, 798, 864
Hpy99I	CGWCG	6 cut(s)	172, 326, 331, 416, 440, 443
HpyCH4III	ACNGT	1 cut(s)	1152
HpyCH4IV	ACGT	1 cut(s)	177
HpyF10VI	GCNNNNNNNGC	7 cut(s)	42, 268, 300, 361, 740, 811, 1831
HpyF3I	CTNAG	5 cut(s)	680, 792, 854, 1248, 1973
HpySE526I	ACGT	1 cut(s)	177
Hsp92II	CATG	8 cut(s)	26, 551, 764, 785, 818, 911, 1032, 1347
HspAI	GCGC	3 cut(s)	45, 147, 314
Ksp22I	TGATCA	3 cut(s)	1080, 1096, 1672
LmnI	GCTCC	2 cut(s)	1443, 1648
Lsp1109I	GCAGC	6 cut(s)	287, 637, 1071, 1538, 1775, 1821
LweI	GCATC	5 cut(s)	613, 637, 697, 1184, 1501
MaeI	CTAG	3 cut(s)	672, 1146, 1584
MaeII	ACGT	1 cut(s)	177
MaeIII	GTNAC	4 cut(s)	929, 1372, 1755, 1982
MboII	GAAGA	5 cut(s)	228, 324, 521, 767, 1044
MflI	RGATCY	2 cut(s)	10, 1858
MhlI	GDGCHC	1 cut(s)	454
MlsI	TGGCCA	1 cut(s)	1033
MluCI	AATT	8 cut(s)	55, 72, 252, 819, 1137, 1258, 1331, 1896
MluI	ACGCGT	1 cut(s)	324
MluNI	TGGCCA	1 cut(s)	1033
MlyI	GAGTC	1 cut(s)	395
MmeI	TCCRAC	2 cut(s)	107, 1750
Mox20I	TGGCCA	1 cut(s)	1033
Mph1103I	ATGCAT	2 cut(s)	484, 690
MscI	TGGCCA	1 cut(s)	1033
MseI	TTAA	6 cut(s)	251, 987, 1013, 1233, 1434, 1560
MslI	CAYNNNNRTG	3 cut(s)	765, 1320, 1633
Msp20I	TGGCCA	1 cut(s)	1033
MspA1I	CMGCKG	1 cut(s)	162
MspCI	CTTAAG	1 cut(s)	986
MspI	CCGG	3 cut(s)	338, 638, 1725
MspR9I	CCNGG	3 cut(s)	429, 639, 1286
Mva1269I	GAATGC	1 cut(s)	701
MvaI	CCWGG	2 cut(s)	429, 1286
MvnI	CGCG	2 cut(s)	262, 326
MwoI	GCNNNNNNNGC	7 cut(s)	42, 268, 300, 361, 740, 811, 1831
NciI	CCSGG	1 cut(s)	639
NcoI	CCATGG	1 cut(s)	1028
NdeI	CATATG	2 cut(s)	1669, 1738
NlaIII	CATG	8 cut(s)	26, 551, 764, 785, 818, 911, 1032, 1347
NlaIV	GGNNCC	3 cut(s)	131, 1183, 1644
NmeAIII	GCCGAG	2 cut(s)	781, 1855
NmuCI	GTSAC	1 cut(s)	1982
NsbI	TGCGCA	2 cut(s)	46, 148
NsiI	ATGCAT	2 cut(s)	484, 690
NspI	RCATGY	3 cut(s)	785, 911, 1347
PaeR7I	CTCGAG	1 cut(s)	1838
PagI	TCATGA	2 cut(s)	547, 760
PcsI	WCGNNNNNNNCGW	1 cut(s)	183
PctI	GAATGC	1 cut(s)	701
PfeI	GAWTC	2 cut(s)	26, 632
PflFI	GACNNNGTC	1 cut(s)	1980
PkrI	GCNGC	8 cut(s)	270, 302, 392, 652, 1061, 1528, 1765, 1836
Ple19I	CGATCG	1 cut(s)	573
PleI	GAGTC	1 cut(s)	395
PpsI	GAGTC	1 cut(s)	395
Ppu21I	YACGTR	1 cut(s)	178
PpuMI	RGGWCCY	2 cut(s)	382, 1182
Psp5II	RGGWCCY	2 cut(s)	382, 1182
Psp6I	CCWGG	2 cut(s)	427, 1284
PspEI	GGTNACC	1 cut(s)	1982
PspFI	CCCAGC	1 cut(s)	158
PspGI	CCWGG	2 cut(s)	427, 1284
PspN4I	GGNNCC	3 cut(s)	131, 1183, 1644
PspPI	GGNCC	3 cut(s)	382, 804, 1182
PspPPI	RGGWCCY	2 cut(s)	382, 1182
PsrI	GAACNNNNNNTAC	4 cut(s)	762, 781, 794, 813
PstNI	CAGNNNCTG	1 cut(s)	1622
PsuI	RGATCY	2 cut(s)	10, 1858
PsyI	GACNNNGTC	1 cut(s)	1980
PvuI	CGATCG	1 cut(s)	573
PvuII	CAGCTG	1 cut(s)	162
RsaI	GTAC	6 cut(s)	588, 780, 1207, 1216, 1329, 1348
RsaNI	GTAC	6 cut(s)	587, 779, 1206, 1215, 1328, 1347
RseI	CAYNNNNRTG	3 cut(s)	765, 1320, 1633
SaqAI	TTAA	6 cut(s)	251, 987, 1013, 1233, 1434, 1560
SatI	GCNGC	8 cut(s)	269, 301, 391, 651, 1060, 1527, 1764, 1835
Sau96I	GGNCC	3 cut(s)	382, 804, 1182
SchI	GAGTC	1 cut(s)	395
ScrFI	CCNGG	3 cut(s)	429, 639, 1286
SduI	GDGCHC	1 cut(s)	454
SfaNI	GCATC	5 cut(s)	613, 637, 697, 1184, 1501
SfcI	CTRYAG	2 cut(s)	627, 1901
Sfr274I	CTCGAG	1 cut(s)	1838
SinI	GGWCC	2 cut(s)	382, 1182
SlaI	CTCGAG	1 cut(s)	1838
SmiMI	CAYNNNNRTG	3 cut(s)	765, 1320, 1633
SmlI	CTYRAG	7 cut(s)	395, 737, 986, 1218, 1241, 1451, 1838
SmoI	CTYRAG	7 cut(s)	395, 737, 986, 1218, 1241, 1451, 1838
Sse9I	AATT	8 cut(s)	55, 72, 252, 819, 1137, 1258, 1331, 1896
SsiI	CCGC	9 cut(s)	34, 225, 269, 303, 388, 391, 580, 878, 1188
SspMI	CTAG	3 cut(s)	672, 1146, 1584
StyD4I	CCNGG	3 cut(s)	427, 637, 1284
StyI	CCWWGG	3 cut(s)	936, 1028, 1473
TaaI	ACNGT	1 cut(s)	1152
TaiI	ACGT	1 cut(s)	180
TaqI	TCGA	7 cut(s)	435, 562, 573, 1312, 1464, 1839, 1967
TasI	AATT	8 cut(s)	55, 72, 252, 819, 1137, 1258, 1331, 1896
TatI	WGTACW	4 cut(s)	586, 778, 1214, 1327
TauI	GCSGC	2 cut(s)	271, 393
TfiI	GAWTC	2 cut(s)	26, 632
Tru1I	TTAA	6 cut(s)	251, 987, 1013, 1233, 1434, 1560
Tru9I	TTAA	6 cut(s)	251, 987, 1013, 1233, 1434, 1560
TseFI	GTSAC	1 cut(s)	1982
TseI	GCWGC	6 cut(s)	300, 650, 1059, 1526, 1763, 1834
Tsp45I	GTSAC	1 cut(s)	1982
TspDTI	ATGAA	7 cut(s)	584, 783, 831, 1324, 1560, 1566, 1755
TspGWI	ACGGA	1 cut(s)	232
Tth111I	GACNNNGTC	1 cut(s)	1980
Vha464I	CTTAAG	1 cut(s)	986
VpaK11BI	GGWCC	2 cut(s)	382, 1182
XagI	CCTNNNNNAGG	1 cut(s)	587
XapI	RAATTY	2 cut(s)	1258, 1896
XceI	RCATGY	3 cut(s)	785, 911, 1347
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1537
XhoI	CTCGAG	1 cut(s)	1838
XspI	CTAG	3 cut(s)	672, 1146, 1584
Zsp2I	ATGCAT	2 cut(s)	484, 690

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.