Rroxscaffold_7G00164440

Tyrosine kinase, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001013
Physical Location & Seq
Reverse (-)
6414858 .. 6422067
7210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00164440.1

Sequence Viewer

Length: 1689 bp
ATGATGGCGGAGGAAGACACGGAGAGTCGGTGTAGCATACCTGCGGGTTTTTCGCCGGCTTTGACCCGAAAGCAGAAACTGAAAGTGAATATCTACAATGAAGTTCTGGTCCGTCTTAAGGACTCGGATGTCGCGGAGGCCCAACAGCCCGGTTTCGAAGACGAACTCTGGGCTCATTTCAATCGCCTCCCAACTAGATATGCTTTGGATGTGAATGTGGAGAGGGCACAAGATGTCCTCATGCATAAAAGACTATTGCATGTGGCACATGACCCTACCACTAGACCTGCAGTCGAAGTCCGTCTTGTGCAGGTTCATTCTTCTTCTAATTTAACCTCATCCAGAAAAGAAGATGCTCAATGTTCTGGTTTTGCTGGCAAACAGAGGCATGGTTCAACAAAAAGCTGTTTGGACTTGGTTTTCTTTATTCATCCACCACCAGCTTTTGGTTTGTCAGAAGACCTTGAACTTGCTCTTGAAGCCAAGAAGTTACAAGTTCAACATGGGAATACCATTGTGAATGCCTTTTCAAGGCCGATGCATGAAATTACAATTTCAACTAATGACAAGCCCAAACTCCTCAGTGAGTTGACATCCTTATTATCTGACATCGGACTGAACATTCAAGAAGCACATGCTTTTTCTACAACAGATGGCTACTCCTTGGATGTCTTTGTCGTTGATGGTTGGGAATGTGAGGAAACTGAACGGCTTAGACAGGCACTGACAAAAGAAATACTATGGAGTGAGAATCCTTGCTTGGATTATAATGTCATCTCCCCTGTTCTGGGGCAAGACACTGGAATGAAGGTCATTGGTGATCATGTAAGCACATCCTGTGAAGAAAAAGATATCTGGGAAATTGAAGCAAGCCTATTGACATATGAAAGGAAAATTGCTTCTGGATCAAATGGTGATTTATATCAAGGTACTTTCTGTGGTCAAGATGTGGCCATTAAAGTTCTTAGCACTGAGGGCCTAAATGAAACCATGAGAGAGTTTACCCAAGAAGTCTCTATCATGAGAAAAGTTCGGCACAAGAATGTCGTTCAATTCATTGGGGCTTGCACTAAACCTCCAAACCTCTGCATAGTTACTGGGTTTCCATTCAGTTCAACTGCTGCTCATATACCTTTTCACTTATCAGAATTTTTGTCTGGTGGAAGCATGTACGACTTTCTCCATAAGCAAAAGGGTTCTTTTTCACTTCAGTCCTTGCTCAAAGTTGCAGTTGATATTTCCAGGGGAATGAAATACTTGCACCAAAATAATATAATCCACAGGGACTTGAAAGCTGCTAATCTTTTGATGGATGGAAATGGAGTTGTGAAAGTCGCTGATTTTGGGATTGCTAGAGTACAGGCACAATCTGGTGTCATGACTGCAGAAACTGGCACCTACCGTTGGATGGCTCCAGAGCTTCCCTATGAGAACTTAACTCCACTACAAGCAGCACTTGGCGTTGTCAAGAAGGGTCTGAGGCCTACGATTCCAAAGCACACTAACCCCATGTTAGTGGAGTTAATGGAGAGTTGCTGGCAGCAAGATGCATCATTGAGACCTGAGTTCTCAGATATTGTAAATATGTTGCGACAAATGGCTAGGAGGGTTGCGGATGAAAGGGAGGATCGACGAAAGGGAAAATCAGCAAAAGTAGTACCTACTCTTAGGCATGGCAGTGCCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MS_channel Pkinase_Tyr

Protein Analysis

562

Amino Acids

62.76

Weight (kDa)

6.2

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 294 - 474 9.5e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 295 - 474 5.7e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 475 - 530 1.6e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 768
AasI GACNNNNNNGTC 1 cut(s) 128
Acc36I ACCTGC 3 cut(s) 49, 295, 301
AccB1I GGYRCC 1 cut(s) 1394
AccB7I CCANNNNNTGG 1 cut(s) 446
AccII CGCG 1 cut(s) 134
AciI CCGC 4 cut(s) 8, 44, 134, 1613
AclWI GGATC 2 cut(s) 913, 1635
AcoI YGGCCR 1 cut(s) 951
AcsI RAATTY 1 cut(s) 1148
AcuI CTGAAG 1 cut(s) 1193
AfaI GTAC 4 cut(s) 931, 1172, 1359, 1659
AfiI CCNNNNNNNGG 7 cut(s) 118, 446, 531, 787, 788, 1404, 1408
AflII CTTAAG 1 cut(s) 116
AhdI GACNNNNNGTC 1 cut(s) 290
AjnI CCWGG 1 cut(s) 1241
AjuI GAANNNNNNNTTGG 2 cut(s) 743, 775
AluBI AGCT 4 cut(s) 405, 443, 1295, 1420
AluI AGCT 4 cut(s) 405, 443, 1295, 1420
Alw26I GTCTC 2 cut(s) 1018, 1552
AlwI GGATC 2 cut(s) 913, 1635
AlwNI CAGNNNCTG 3 cut(s) 79, 724, 1391
AoxI GGCC 5 cut(s) 138, 533, 951, 976, 1481
ApeKI GCWGC 4 cut(s) 1121, 1295, 1451, 1540
ApoI RAATTY 1 cut(s) 1148
AspS9I GGNCC 3 cut(s) 109, 139, 976
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 2 cut(s) 830, 926
AsuII TTCGAA 1 cut(s) 156
AvaII GGWCC 1 cut(s) 109
BaeGI GKGCMC 1 cut(s) 229
BalI TGGCCA 1 cut(s) 953
BanI GGYRCC 1 cut(s) 1394
BanII GRGCYC 1 cut(s) 175
BbsI GAAGAC 3 cut(s) 21, 165, 465
BbvI GCAGC 4 cut(s) 1108, 1282, 1463, 1552
BccI CCATC 5 cut(s) 647, 677, 1303, 1307, 1402
BceAI ACGGC 1 cut(s) 725
BciT130I CCWGG 1 cut(s) 1243
BclI TGATCA 1 cut(s) 820
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 2 cut(s) 1018, 1552
BfaI CTAG 4 cut(s) 195, 282, 1353, 1602
BfmI CTRYAG 2 cut(s) 288, 1383
BfrI CTTAAG 1 cut(s) 116
BfuAI ACCTGC 3 cut(s) 49, 295, 301
BisI GCNGC 4 cut(s) 1122, 1296, 1452, 1541
BlsI GCNGC 4 cut(s) 1123, 1297, 1453, 1542
Bme1390I CCNGG 2 cut(s) 150, 1243
Bme18I GGWCC 1 cut(s) 109
BmeRI GACNNNNNGTC 1 cut(s) 290
BmgT120I GGNCC 3 cut(s) 109, 139, 976
BmiI GGNNCC 2 cut(s) 1396, 1413
BmrFI CCNGG 2 cut(s) 150, 1243
BmrI ACTGGG 1 cut(s) 1107
BmsI GCATC 4 cut(s) 343, 528, 1537, 1559
BmuI ACTGGG 1 cut(s) 1107
BpiI GAAGAC 3 cut(s) 21, 165, 465
BpmI CTGGAG 1 cut(s) 1398
Bpu14I TTCGAA 1 cut(s) 156
BpuMI CCSGG 1 cut(s) 150
BsaBI GATNNNNATC 1 cut(s) 921
BsaI GGTCTC 1 cut(s) 1552
BsaJI CCNNGG 2 cut(s) 663, 1242
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 1060, 1090
Bsc4I CCNNNNNNNGG 7 cut(s) 118, 446, 531, 787, 788, 1404, 1408
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 3 cut(s) 805, 1102, 1396
Bse8I GATNNNNATC 1 cut(s) 921
BseBI CCWGG 1 cut(s) 1243
BseDI CCNNGG 2 cut(s) 663, 1242
BseJI GATNNNNATC 1 cut(s) 921
BseLI CCNNNNNNNGG 7 cut(s) 118, 446, 531, 787, 788, 1404, 1408
BseMII CTCAG 5 cut(s) 595, 963, 1469, 1554, 1584
BseNI ACTGG 3 cut(s) 805, 1102, 1396
BseRI GAGGAG 1 cut(s) 569
BseSI GKGCMC 1 cut(s) 229
BseXI GCAGC 4 cut(s) 1108, 1282, 1463, 1552
BsgI GTGCAG 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 134
BshFI GGCC 5 cut(s) 140, 535, 953, 978, 1483
BshNI GGYRCC 1 cut(s) 1394
BsiSI CCGG 2 cut(s) 56, 150
BslFI GGGAC 1 cut(s) 1298
BslI CCNNNNNNNGG 7 cut(s) 118, 446, 531, 787, 788, 1404, 1408
BsmAI GTCTC 2 cut(s) 1018, 1552
BsmFI GGGAC 1 cut(s) 1298
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 5 cut(s) 140, 535, 953, 978, 1483
Bso31I GGTCTC 1 cut(s) 1552
Bsp119I TTCGAA 1 cut(s) 156
Bsp1286I GDGCHC 2 cut(s) 175, 229
Bsp143I GATC 3 cut(s) 820, 905, 1627
BspACI CCGC 4 cut(s) 8, 44, 134, 1613
BspANI GGCC 5 cut(s) 140, 535, 953, 978, 1483
BspCNI CTCAG 5 cut(s) 594, 964, 1470, 1555, 1583
BspFNI CGCG 1 cut(s) 134
BspHI TCATGA 2 cut(s) 1020, 1377
BspLI GGNNCC 2 cut(s) 1396, 1413
BspMAI CTGCAG 2 cut(s) 292, 1387
BspMI ACCTGC 3 cut(s) 49, 295, 301
BspPI GGATC 2 cut(s) 913, 1635
BspT104I TTCGAA 1 cut(s) 156
BspT107I GGYRCC 1 cut(s) 1394
BspTI CTTAAG 1 cut(s) 116
BspTNI GGTCTC 1 cut(s) 1552
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 3 cut(s) 805, 1102, 1396
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 663, 1242
BssMI GATC 3 cut(s) 820, 905, 1627
BssT1I CCWWGG 1 cut(s) 663
Bst2UI CCWGG 1 cut(s) 1243
Bst4CI ACNGT 1 cut(s) 1403
BstAFI CTTAAG 1 cut(s) 116
BstBI TTCGAA 1 cut(s) 156
BstC8I GCNNGC 5 cut(s) 57, 376, 871, 1066, 1538
BstDEI CTNAG 8 cut(s) 581, 713, 965, 972, 1478, 1563, 1570, 1667
BstENI CCTNNNNNAGG 1 cut(s) 529
BstFNI CGCG 1 cut(s) 134
BstKTI GATC 3 cut(s) 823, 908, 1630
BstMAI GTCTC 2 cut(s) 1018, 1552
BstMBI GATC 3 cut(s) 820, 905, 1627
BstMWI GCNNNNNNNGC 2 cut(s) 479, 975
BstNI CCWGG 1 cut(s) 1243
BstNSI RCATGY 3 cut(s) 263, 638, 1171
BstSCI CCNGG 2 cut(s) 148, 1241
BstSFI CTRYAG 2 cut(s) 288, 1383
BstSLI GKGCMC 1 cut(s) 229
BstUI CGCG 1 cut(s) 134
BstV1I GCAGC 4 cut(s) 1108, 1282, 1463, 1552
BstV2I GAAGAC 3 cut(s) 21, 165, 465
BstXI CCANNNNNNTGG 1 cut(s) 1516
BsuRI GGCC 5 cut(s) 140, 535, 953, 978, 1483
BtsI GCAGTG 1 cut(s) 1684
BtsIMutI CAGTG 5 cut(s) 589, 722, 798, 969, 1684
BveI ACCTGC 3 cut(s) 49, 295, 301
Cac8I GCNNGC 5 cut(s) 57, 376, 871, 1066, 1538
CaiI CAGNNNCTG 3 cut(s) 79, 724, 1391
CciI TCATGA 2 cut(s) 1020, 1377
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 3 cut(s) 109, 139, 976
Csp6I GTAC 4 cut(s) 930, 1171, 1358, 1658
CviQI GTAC 4 cut(s) 930, 1171, 1358, 1658
DdeI CTNAG 8 cut(s) 581, 713, 965, 972, 1478, 1563, 1570, 1667
DpnI GATC 3 cut(s) 822, 907, 1629
DpnII GATC 3 cut(s) 820, 905, 1627
DrdI GACNNNNNNGTC 1 cut(s) 128
DriI GACNNNNNGTC 1 cut(s) 290
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 1 cut(s) 951
Eam1105I GACNNNNNGTC 1 cut(s) 290
EciI GGCGGA 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 663
Eco147I AGGCCT 1 cut(s) 1483
Eco24I GRGCYC 1 cut(s) 175
Eco31I GGTCTC 1 cut(s) 1552
Eco32I GATATC 1 cut(s) 853
Eco47I GGWCC 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 1193
EcoNI CCTNNNNNAGG 1 cut(s) 529
EcoO109I RGGNCCY 1 cut(s) 976
EcoRII CCWGG 1 cut(s) 1241
EcoRV GATATC 1 cut(s) 853
EcoT14I CCWWGG 1 cut(s) 663
EcoT22I ATGCAT 3 cut(s) 246, 543, 1552
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 663
FalI AAGNNNNNCTT 4 cut(s) 288, 320, 1440, 1472
FaqI GGGAC 1 cut(s) 1298
FauI CCCGC 1 cut(s) 37
FauNDI CATATG 1 cut(s) 883
FbaI TGATCA 1 cut(s) 820
Fnu4HI GCNGC 4 cut(s) 1122, 1296, 1452, 1541
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 4 cut(s) 1122, 1296, 1452, 1541
FspBI CTAG 4 cut(s) 195, 282, 1353, 1602
GluI GCNGC 4 cut(s) 1122, 1296, 1452, 1541
GsuI CTGGAG 1 cut(s) 1398
HaeIII GGCC 5 cut(s) 140, 535, 953, 978, 1483
HapII CCGG 2 cut(s) 56, 150
HincII GTYRAC 1 cut(s) 591
HindII GTYRAC 1 cut(s) 591
HinfI GANTC 4 cut(s) 25, 122, 751, 1489
HpaII CCGG 2 cut(s) 56, 150
HphI GGTGA 2 cut(s) 830, 926
Hpy166II GTNNAC 2 cut(s) 591, 1002
Hpy188I TCNGA 7 cut(s) 127, 457, 607, 614, 1147, 1479, 1573
Hpy188III TCNNGA 9 cut(s) 342, 476, 626, 903, 944, 1021, 1378, 1415, 1468
Hpy8I GTNNAC 2 cut(s) 591, 1002
Hpy99I CGWCG 1 cut(s) 1635
HpyAV CCTTC 2 cut(s) 802, 1465
HpyCH4III ACNGT 1 cut(s) 1403
HpyF10VI GCNNNNNNNGC 2 cut(s) 479, 975
HpyF3I CTNAG 8 cut(s) 581, 713, 965, 972, 1478, 1563, 1570, 1667
KroI GCCGGC 1 cut(s) 55
KroNI GCCGGC 1 cut(s) 57
Ksp22I TGATCA 1 cut(s) 820
Kzo9I GATC 3 cut(s) 820, 905, 1627
LmnI GCTCC 1 cut(s) 1417
Lsp1109I GCAGC 4 cut(s) 1108, 1282, 1463, 1552
LweI GCATC 4 cut(s) 343, 528, 1537, 1559
MaeI CTAG 4 cut(s) 195, 282, 1353, 1602
MaeIII GTNAC 2 cut(s) 489, 1093
MalI GATC 3 cut(s) 822, 907, 1629
MboI GATC 3 cut(s) 820, 905, 1627
MboII GAAGA 7 cut(s) 26, 170, 312, 315, 362, 470, 854
MhlI GDGCHC 2 cut(s) 175, 229
MlsI TGGCCA 1 cut(s) 953
MluCI AATT 7 cut(s) 328, 546, 552, 861, 894, 1052, 1148
MluNI TGGCCA 1 cut(s) 953
MlyI GAGTC 2 cut(s) 34, 116
MmeI TCCRAC 1 cut(s) 1385
Mox20I TGGCCA 1 cut(s) 953
Mph1103I ATGCAT 3 cut(s) 246, 543, 1552
MroNI GCCGGC 1 cut(s) 55
MscI TGGCCA 1 cut(s) 953
MseI TTAA 5 cut(s) 117, 332, 957, 1436, 1523
MslI CAYNNNNRTG 4 cut(s) 803, 1041, 1514, 1677
Msp20I TGGCCA 1 cut(s) 953
MspCI CTTAAG 1 cut(s) 116
MspI CCGG 2 cut(s) 56, 150
MspR9I CCNGG 2 cut(s) 150, 1243
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 1 cut(s) 1243
MvnI CGCG 1 cut(s) 134
MwoI GCNNNNNNNGC 2 cut(s) 479, 975
NaeI GCCGGC 1 cut(s) 57
NciI CCSGG 1 cut(s) 150
NdeI CATATG 1 cut(s) 883
NdeII GATC 3 cut(s) 820, 905, 1627
NgoMIV GCCGGC 1 cut(s) 55
NlaIV GGNNCC 2 cut(s) 1396, 1413
NsiI ATGCAT 3 cut(s) 246, 543, 1552
NspI RCATGY 3 cut(s) 263, 638, 1171
NspV TTCGAA 1 cut(s) 156
PagI TCATGA 2 cut(s) 1020, 1377
PceI AGGCCT 1 cut(s) 1483
PctI GAATGC 1 cut(s) 526
PdiI GCCGGC 1 cut(s) 57
PfeI GAWTC 2 cut(s) 751, 1489
PflMI CCANNNNNTGG 1 cut(s) 446
PkrI GCNGC 4 cut(s) 1123, 1297, 1453, 1542
PleI GAGTC 2 cut(s) 33, 116
PpsI GAGTC 2 cut(s) 33, 116
PsiI TTATAA 1 cut(s) 768
Psp6I CCWGG 1 cut(s) 1241
PspGI CCWGG 1 cut(s) 1241
PspN4I GGNNCC 2 cut(s) 1396, 1413
PspPI GGNCC 3 cut(s) 109, 139, 976
PstI CTGCAG 2 cut(s) 292, 1387
PstNI CAGNNNCTG 3 cut(s) 79, 724, 1391
RsaI GTAC 4 cut(s) 931, 1172, 1359, 1659
RsaNI GTAC 4 cut(s) 930, 1171, 1358, 1658
RseI CAYNNNNRTG 4 cut(s) 803, 1041, 1514, 1677
SaqAI TTAA 5 cut(s) 117, 332, 957, 1436, 1523
SatI GCNGC 4 cut(s) 1122, 1296, 1452, 1541
Sau3AI GATC 3 cut(s) 820, 905, 1627
Sau96I GGNCC 3 cut(s) 109, 139, 976
SchI GAGTC 2 cut(s) 34, 116
ScrFI CCNGG 2 cut(s) 150, 1243
SduI GDGCHC 2 cut(s) 175, 229
SfaNI GCATC 4 cut(s) 343, 528, 1537, 1559
SfcI CTRYAG 2 cut(s) 288, 1383
SfuI TTCGAA 1 cut(s) 156
SinI GGWCC 1 cut(s) 109
SmiMI CAYNNNNRTG 4 cut(s) 803, 1041, 1514, 1677
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 7 cut(s) 328, 546, 552, 861, 894, 1052, 1148
SseBI AGGCCT 1 cut(s) 1483
SsiI CCGC 4 cut(s) 8, 44, 134, 1613
SspMI CTAG 4 cut(s) 195, 282, 1353, 1602
StuI AGGCCT 1 cut(s) 1483
StyD4I CCNGG 2 cut(s) 148, 1241
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 1 cut(s) 1403
TaqI TCGA 3 cut(s) 156, 294, 1630
TasI AATT 7 cut(s) 328, 546, 552, 861, 894, 1052, 1148
TatI WGTACW 1 cut(s) 1357
TfiI GAWTC 2 cut(s) 751, 1489
Tru1I TTAA 5 cut(s) 117, 332, 957, 1436, 1523
Tru9I TTAA 5 cut(s) 117, 332, 957, 1436, 1523
TscAI CASTG 5 cut(s) 589, 729, 805, 976, 1684
TseI GCWGC 4 cut(s) 1121, 1295, 1451, 1540
TspGWI ACGGA 3 cut(s) 35, 101, 290
TspRI CASTG 5 cut(s) 589, 729, 805, 976, 1684
Van91I CCANNNNNTGG 1 cut(s) 446
Vha464I CTTAAG 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 109
XagI CCTNNNNNAGG 1 cut(s) 529
XapI RAATTY 1 cut(s) 1148
XceI RCATGY 3 cut(s) 263, 638, 1171
XspI CTAG 4 cut(s) 195, 282, 1353, 1602
Zsp2I ATGCAT 3 cut(s) 246, 543, 1552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.