RLG00000011050

Tyrosine kinase, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001013
Physical Location & Seq
Reverse (-)
6334410 .. 6341176
6767 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011050

Sequence Viewer

Length: 1698 bp
ATGCGACAGAGGTCATCAGGAAATGATCGGCGAGGAGAAATAAGGACGAAAAGGGACCACGGGACCCCAGGTTTGAGTTGCGATCAAGAATCGGAACGCACCGTTTTGTATGGTGGTGGTGATATTATGGCGGAGGAAGACACGGAGAGTCGGTGTAGCATACCGGCGGGTTTTTCGCCGGCTTTGACCCGAAAGCAGAAACTGAAAGTGAATATCTACAATGAAGTTCTGGTCCGGCTTAAGGACTCGGATGTCGCGGAGGCCCAACAGCCCGGTTTCGAAGACGAACTCTGGGCTCATTTCAATCGCCTCCCAACTAGGTATGCTTTGGATGTGAACGTGGAGAGGGCACAAGATGTCCTCATGCATAAAAGACTATTGCATGTGGCACATGACCCTGCCACTAGACCTGCAGTCGAAGTCCGTCTTGTGCAGGTTCATTCTTCTTCTAATGTAAGCTCATCCAGAAAAGAAGATACTCAGTGTTCTGGTTTTGCTGACAAACAGAGGCCGATGCATGAAATTACAATTTCAACTAATGACAAGCCTAAACTCCTCAGTGAGTTGACATCCTTATTATCTGACATCGGACTGAACATTCAAGAAGCACATGCTTTTTCTACAACGGATGGCTACTCCTTGGATGTCTTTGTCGTTGATGGTTGGGAATGTGAGGAAACTGAACGGCTTAGACAGGCACTGGCAAAAGAAATACTATGGAGTGAGAATCCTTGCGTGGATTATAATGTCATATCTCCTGTTCTGGGGCAAGACACTGGAATGAAGGTCATTGGTGATCATGTAAGCACATCCAGTGAAGAAAAAGATATCTGGGAAATTGAAGCAAGCCTATTGACATATGAAAGGAAAATTGCTTCTGGATCAAATGGTGATTTATATCAAGGTACTTTCTGTGGTCAAGATGTGGCCATTAAAGTTCTTAGCACTGAGCGCCTAAACGAAACCATGAGGGAGTTTACCCAAGAAGTCTCTATCATGAGAAAAGTTCGGCACAAGAATGTCGTTCAATTCATTGGGGCTTGCACTAAACCTCCTAACCTTTGCATAGTTACTGAATTTTTGTCTGGTGGAAGCATGTACGACTTTCTCCATAAGCAAAAGGGTTCTTTTTCACTTCAGTCCTTGCTCAAAGTTGCAGTCGGTATTTCCAGGGGAATGAAATACTTGCACCAAAATAATATAATCCACAGGGACTTGAAAGCTGCTAATCTTTTGATGGATGGAAATGGAGTTGTGAAAGTAGCTGATTTTGGGATTGCAAGAGTACAGGCACAATCTGGTGTCATGACTGCAGAAACTGGCACCTACCGTTGGATGGCTCCAGAGGTCATAGAACATAAACCTTATGATCACAAAGCCGATGTTTTTAGCTTTGGGGTTATTCTGTGGGAGCTTCTTACAGGAAAGCTTCCCTATGAGAACTTAACTCCACTACAAGCAGCACTTGGCGTTGTCAAGAAGGGTCTGAGGCCTACGATTCCAAAGCACACTAACCCCATGTTAGTGGAGTTAATGGAGAGTTGCTGGCAGCAAGATGCATCGTTGAGACCTGAGTTCTCAGATATTGTAAATATGTTGCGACAAATGGCTAGGAGGGTTGCGGATGAAAGGGAGGATCGACGAAAGGGAAAATCAGCAAAAGTAGTACCCACTCTCAGGCATGGCAGTGCCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MS_channel Pkinase_Tyr

Protein Analysis

566

Amino Acids

63.38

Weight (kDa)

6.31

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 286 - 533 7e-74 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 287 - 531 9e-59 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
AasI GACNNNNNNGTC 1 cut(s) 251
Acc36I ACCTGC 2 cut(s) 418, 424
AccB1I GGYRCC 1 cut(s) 1322
AccII CGCG 1 cut(s) 257
AciI CCGC 4 cut(s) 131, 167, 257, 1622
AclWI GGATC 2 cut(s) 889, 1644
AcoI YGGCCR 1 cut(s) 927
AcsI RAATTY 1 cut(s) 1076
AcuI CTGAAG 1 cut(s) 1121
AfaI GTAC 4 cut(s) 907, 1100, 1287, 1668
AfiI CCNNNNNNNGG 5 cut(s) 241, 764, 1332, 1336, 1677
AflII CTTAAG 1 cut(s) 239
AgsI TTSAA 6 cut(s) 304, 534, 602, 842, 1028, 1219
AhdI GACNNNNNGTC 1 cut(s) 413
AjnI CCWGG 2 cut(s) 67, 1169
AluBI AGCT 6 cut(s) 459, 1223, 1265, 1392, 1414, 1429
AluI AGCT 6 cut(s) 459, 1223, 1265, 1392, 1414, 1429
Alw26I GTCTC 2 cut(s) 994, 1561
AlwI GGATC 2 cut(s) 889, 1644
AlwNI CAGNNNCTG 3 cut(s) 202, 700, 1319
AoxI GGCC 4 cut(s) 261, 509, 927, 1490
ApeKI GCWGC 3 cut(s) 1223, 1460, 1549
ApoI RAATTY 1 cut(s) 1076
ArsI GACNNNNNNTTYG 4 cut(s) 55, 87, 1143, 1175
AspLEI GCGC 1 cut(s) 954
AspS9I GGNCC 4 cut(s) 55, 63, 232, 262
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 3 cut(s) 131, 806, 902
AsuII TTCGAA 1 cut(s) 279
AvaII GGWCC 3 cut(s) 55, 63, 232
BaeGI GKGCMC 1 cut(s) 352
BalI TGGCCA 1 cut(s) 929
BanI GGYRCC 1 cut(s) 1322
BanII GRGCYC 1 cut(s) 298
BbsI GAAGAC 2 cut(s) 144, 288
BbvI GCAGC 3 cut(s) 1210, 1472, 1561
BccI CCATC 5 cut(s) 623, 653, 1231, 1235, 1330
BceAI ACGGC 1 cut(s) 701
BciT130I CCWGG 2 cut(s) 69, 1171
BclI TGATCA 2 cut(s) 796, 1369
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 2 cut(s) 994, 1561
BfaI CTAG 3 cut(s) 318, 405, 1611
BfmI CTRYAG 2 cut(s) 411, 1311
BfoI RGCGCY 1 cut(s) 955
BfrI CTTAAG 1 cut(s) 239
BfuAI ACCTGC 2 cut(s) 418, 424
BisI GCNGC 3 cut(s) 1224, 1461, 1550
BlsI GCNGC 3 cut(s) 1225, 1462, 1551
Bme1390I CCNGG 3 cut(s) 69, 273, 1171
Bme18I GGWCC 3 cut(s) 55, 63, 232
BmeRI GACNNNNNGTC 1 cut(s) 413
BmgT120I GGNCC 4 cut(s) 55, 63, 232, 262
BmiI GGNNCC 5 cut(s) 56, 64, 65, 1324, 1341
BmrFI CCNGG 3 cut(s) 69, 273, 1171
BmsI GCATC 3 cut(s) 504, 1546, 1568
BoxI GACNNNNGTC 1 cut(s) 10
BpiI GAAGAC 2 cut(s) 144, 288
BpmI CTGGAG 1 cut(s) 1326
Bpu14I TTCGAA 1 cut(s) 279
BpuMI CCSGG 1 cut(s) 273
BsaBI GATNNNNATC 1 cut(s) 897
BsaI GGTCTC 1 cut(s) 1561
BsaJI CCNNGG 4 cut(s) 58, 67, 639, 1170
BsaXI ACNNNNNCTCC 4 cut(s) 137, 167, 1036, 1066
Bsc4I CCNNNNNNNGG 5 cut(s) 241, 764, 1332, 1336, 1677
Bse118I RCCGGY 2 cut(s) 163, 178
Bse1I ACTGG 4 cut(s) 705, 781, 813, 1324
Bse8I GATNNNNATC 1 cut(s) 897
BseBI CCWGG 2 cut(s) 69, 1171
BseDI CCNNGG 4 cut(s) 58, 67, 639, 1170
BseJI GATNNNNATC 1 cut(s) 897
BseLI CCNNNNNNNGG 5 cut(s) 241, 764, 1332, 1336, 1677
BseMII CTCAG 7 cut(s) 494, 571, 939, 1478, 1563, 1593, 1690
BseNI ACTGG 4 cut(s) 705, 781, 813, 1324
BseRI GAGGAG 2 cut(s) 48, 545
BseSI GKGCMC 1 cut(s) 352
BseXI GCAGC 3 cut(s) 1210, 1472, 1561
BsgI GTGCAG 1 cut(s) 452
Bsh1236I CGCG 1 cut(s) 257
BshFI GGCC 4 cut(s) 263, 511, 929, 1492
BshNI GGYRCC 1 cut(s) 1322
BsiSI CCGG 4 cut(s) 164, 179, 235, 273
BslFI GGGAC 3 cut(s) 68, 76, 1226
BslI CCNNNNNNNGG 5 cut(s) 241, 764, 1332, 1336, 1677
BsmAI GTCTC 2 cut(s) 994, 1561
BsmFI GGGAC 3 cut(s) 68, 76, 1226
BsnI GGCC 4 cut(s) 263, 511, 929, 1492
Bso31I GGTCTC 1 cut(s) 1561
Bsp119I TTCGAA 1 cut(s) 279
Bsp1286I GDGCHC 2 cut(s) 298, 352
Bsp143I GATC 6 cut(s) 25, 82, 796, 881, 1369, 1636
BspACI CCGC 4 cut(s) 131, 167, 257, 1622
BspANI GGCC 4 cut(s) 263, 511, 929, 1492
BspCNI CTCAG 7 cut(s) 493, 570, 940, 1479, 1564, 1592, 1689
BspFNI CGCG 1 cut(s) 257
BspHI TCATGA 2 cut(s) 996, 1305
BspLI GGNNCC 5 cut(s) 56, 64, 65, 1324, 1341
BspMAI CTGCAG 2 cut(s) 415, 1315
BspMI ACCTGC 2 cut(s) 418, 424
BspPI GGATC 2 cut(s) 889, 1644
BspT104I TTCGAA 1 cut(s) 279
BspT107I GGYRCC 1 cut(s) 1322
BspTI CTTAAG 1 cut(s) 239
BspTNI GGTCTC 1 cut(s) 1561
BsrFI RCCGGY 2 cut(s) 163, 178
BsrI ACTGG 4 cut(s) 705, 781, 813, 1324
BssAI RCCGGY 2 cut(s) 163, 178
BssECI CCNNGG 4 cut(s) 58, 67, 639, 1170
BssMI GATC 6 cut(s) 25, 82, 796, 881, 1369, 1636
BssT1I CCWWGG 1 cut(s) 639
Bst2UI CCWGG 2 cut(s) 69, 1171
Bst4CI ACNGT 2 cut(s) 103, 1331
BstAFI CTTAAG 1 cut(s) 239
BstBI TTCGAA 1 cut(s) 279
BstC8I GCNNGC 4 cut(s) 180, 847, 1042, 1547
BstDEI CTNAG 9 cut(s) 480, 557, 689, 941, 948, 1487, 1572, 1579, 1676
BstDSI CCRYGG 1 cut(s) 58
BstFNI CGCG 1 cut(s) 257
BstH2I RGCGCY 1 cut(s) 955
BstHHI GCGC 1 cut(s) 954
BstKTI GATC 6 cut(s) 28, 85, 799, 884, 1372, 1639
BstMAI GTCTC 2 cut(s) 994, 1561
BstMBI GATC 6 cut(s) 25, 82, 796, 881, 1369, 1636
BstMWI GCNNNNNNNGC 1 cut(s) 951
BstNI CCWGG 2 cut(s) 69, 1171
BstNSI RCATGY 3 cut(s) 386, 614, 1099
BstPAI GACNNNNGTC 1 cut(s) 10
BstSCI CCNGG 3 cut(s) 67, 271, 1169
BstSFI CTRYAG 2 cut(s) 411, 1311
BstSLI GKGCMC 1 cut(s) 352
BstUI CGCG 1 cut(s) 257
BstV1I GCAGC 3 cut(s) 1210, 1472, 1561
BstV2I GAAGAC 2 cut(s) 144, 288
BstXI CCANNNNNNTGG 1 cut(s) 1525
BsuRI GGCC 4 cut(s) 263, 511, 929, 1492
BtgI CCRYGG 1 cut(s) 58
BtsI GCAGTG 1 cut(s) 1693
BtsIMutI CAGTG 7 cut(s) 488, 565, 698, 774, 820, 945, 1693
BveI ACCTGC 2 cut(s) 418, 424
Cac8I GCNNGC 4 cut(s) 180, 847, 1042, 1547
CaiI CAGNNNCTG 3 cut(s) 202, 700, 1319
CciI TCATGA 2 cut(s) 996, 1305
CfoI GCGC 1 cut(s) 954
Cfr10I RCCGGY 2 cut(s) 163, 178
Cfr13I GGNCC 4 cut(s) 55, 63, 232, 262
Csp6I GTAC 4 cut(s) 906, 1099, 1286, 1667
CviQI GTAC 4 cut(s) 906, 1099, 1286, 1667
DdeI CTNAG 9 cut(s) 480, 557, 689, 941, 948, 1487, 1572, 1579, 1676
DpnI GATC 6 cut(s) 27, 84, 798, 883, 1371, 1638
DpnII GATC 6 cut(s) 25, 82, 796, 881, 1369, 1636
DrdI GACNNNNNNGTC 1 cut(s) 251
DriI GACNNNNNGTC 1 cut(s) 413
DseDI GACNNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 1 cut(s) 927
Eam1105I GACNNNNNGTC 1 cut(s) 413
EciI GGCGGA 1 cut(s) 146
Eco130I CCWWGG 1 cut(s) 639
Eco147I AGGCCT 1 cut(s) 1492
Eco24I GRGCYC 1 cut(s) 298
Eco31I GGTCTC 1 cut(s) 1561
Eco32I GATATC 1 cut(s) 829
Eco47I GGWCC 3 cut(s) 55, 63, 232
Eco57I CTGAAG 1 cut(s) 1121
EcoO109I RGGNCCY 1 cut(s) 63
EcoRII CCWGG 2 cut(s) 67, 1169
EcoRV GATATC 1 cut(s) 829
EcoT14I CCWWGG 1 cut(s) 639
EcoT22I ATGCAT 3 cut(s) 369, 519, 1561
EcoT38I GRGCYC 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 639
FalI AAGNNNNNCTT 4 cut(s) 411, 443, 1449, 1481
FaqI GGGAC 3 cut(s) 68, 76, 1226
FauI CCCGC 1 cut(s) 160
FauNDI CATATG 1 cut(s) 859
FbaI TGATCA 2 cut(s) 796, 1369
Fnu4HI GCNGC 3 cut(s) 1224, 1461, 1550
FriOI GRGCYC 1 cut(s) 298
Fsp4HI GCNGC 3 cut(s) 1224, 1461, 1550
FspBI CTAG 3 cut(s) 318, 405, 1611
GlaI GCGC 1 cut(s) 953
GluI GCNGC 3 cut(s) 1224, 1461, 1550
GsuI CTGGAG 1 cut(s) 1326
HaeII RGCGCY 1 cut(s) 955
HaeIII GGCC 4 cut(s) 263, 511, 929, 1492
HapII CCGG 4 cut(s) 164, 179, 235, 273
HhaI GCGC 1 cut(s) 954
Hin6I GCGC 1 cut(s) 952
HinP1I GCGC 1 cut(s) 952
HincII GTYRAC 1 cut(s) 567
HindII GTYRAC 1 cut(s) 567
HindIII AAGCTT 1 cut(s) 1427
HinfI GANTC 5 cut(s) 89, 148, 245, 727, 1498
HpaII CCGG 4 cut(s) 164, 179, 235, 273
HphI GGTGA 3 cut(s) 131, 806, 902
Hpy166II GTNNAC 3 cut(s) 337, 567, 978
Hpy188I TCNGA 6 cut(s) 94, 250, 583, 590, 1488, 1582
Hpy8I GTNNAC 3 cut(s) 337, 567, 978
Hpy99I CGWCG 1 cut(s) 1644
HpyAV CCTTC 2 cut(s) 778, 1474
HpyCH4III ACNGT 2 cut(s) 103, 1331
HpyCH4IV ACGT 1 cut(s) 339
HpyF10VI GCNNNNNNNGC 1 cut(s) 951
HpyF3I CTNAG 9 cut(s) 480, 557, 689, 941, 948, 1487, 1572, 1579, 1676
HpySE526I ACGT 1 cut(s) 339
HspAI GCGC 1 cut(s) 952
KflI GGGWCCC 1 cut(s) 63
KroI GCCGGC 1 cut(s) 178
KroNI GCCGGC 1 cut(s) 180
Ksp22I TGATCA 2 cut(s) 796, 1369
Kzo9I GATC 6 cut(s) 25, 82, 796, 881, 1369, 1636
LmnI GCTCC 2 cut(s) 1345, 1411
Lsp1109I GCAGC 3 cut(s) 1210, 1472, 1561
LweI GCATC 3 cut(s) 504, 1546, 1568
MaeI CTAG 3 cut(s) 318, 405, 1611
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 1 cut(s) 1069
MalI GATC 6 cut(s) 27, 84, 798, 883, 1371, 1638
MboI GATC 6 cut(s) 25, 82, 796, 881, 1369, 1636
MboII GAAGA 6 cut(s) 149, 293, 435, 438, 485, 830
MhlI GDGCHC 2 cut(s) 298, 352
MlsI TGGCCA 1 cut(s) 929
MluCI AATT 6 cut(s) 522, 528, 837, 870, 1028, 1076
MluNI TGGCCA 1 cut(s) 929
MlyI GAGTC 2 cut(s) 157, 239
MmeI TCCRAC 1 cut(s) 1313
Mox20I TGGCCA 1 cut(s) 929
Mph1103I ATGCAT 3 cut(s) 369, 519, 1561
MroNI GCCGGC 1 cut(s) 178
MscI TGGCCA 1 cut(s) 929
MseI TTAA 4 cut(s) 240, 933, 1445, 1532
MslI CAYNNNNRTG 4 cut(s) 779, 1017, 1523, 1686
Msp20I TGGCCA 1 cut(s) 929
MspCI CTTAAG 1 cut(s) 239
MspI CCGG 4 cut(s) 164, 179, 235, 273
MspR9I CCNGG 3 cut(s) 69, 273, 1171
MvaI CCWGG 2 cut(s) 69, 1171
MvnI CGCG 1 cut(s) 257
MwoI GCNNNNNNNGC 1 cut(s) 951
NaeI GCCGGC 1 cut(s) 180
NciI CCSGG 1 cut(s) 273
NdeI CATATG 1 cut(s) 859
NdeII GATC 6 cut(s) 25, 82, 796, 881, 1369, 1636
NgoMIV GCCGGC 1 cut(s) 178
NlaIV GGNNCC 5 cut(s) 56, 64, 65, 1324, 1341
NsiI ATGCAT 3 cut(s) 369, 519, 1561
NspI RCATGY 3 cut(s) 386, 614, 1099
NspV TTCGAA 1 cut(s) 279
PagI TCATGA 2 cut(s) 996, 1305
PceI AGGCCT 1 cut(s) 1492
PdiI GCCGGC 1 cut(s) 180
PfeI GAWTC 3 cut(s) 89, 727, 1498
PkrI GCNGC 3 cut(s) 1225, 1462, 1551
PleI GAGTC 2 cut(s) 156, 239
PpsI GAGTC 2 cut(s) 156, 239
PpuMI RGGWCCY 1 cut(s) 63
PshAI GACNNNNGTC 1 cut(s) 10
PsiI TTATAA 1 cut(s) 744
Psp5II RGGWCCY 1 cut(s) 63
Psp6I CCWGG 2 cut(s) 67, 1169
PspGI CCWGG 2 cut(s) 67, 1169
PspN4I GGNNCC 5 cut(s) 56, 64, 65, 1324, 1341
PspPI GGNCC 4 cut(s) 55, 63, 232, 262
PspPPI RGGWCCY 1 cut(s) 63
PstI CTGCAG 2 cut(s) 415, 1315
PstNI CAGNNNCTG 3 cut(s) 202, 700, 1319
RsaI GTAC 4 cut(s) 907, 1100, 1287, 1668
RsaNI GTAC 4 cut(s) 906, 1099, 1286, 1667
RseI CAYNNNNRTG 4 cut(s) 779, 1017, 1523, 1686
SaqAI TTAA 4 cut(s) 240, 933, 1445, 1532
SatI GCNGC 3 cut(s) 1224, 1461, 1550
Sau3AI GATC 6 cut(s) 25, 82, 796, 881, 1369, 1636
Sau96I GGNCC 4 cut(s) 55, 63, 232, 262
SchI GAGTC 2 cut(s) 157, 239
ScrFI CCNGG 3 cut(s) 69, 273, 1171
SduI GDGCHC 2 cut(s) 298, 352
SfaNI GCATC 3 cut(s) 504, 1546, 1568
SfcI CTRYAG 2 cut(s) 411, 1311
SfuI TTCGAA 1 cut(s) 279
SinI GGWCC 3 cut(s) 55, 63, 232
SmiMI CAYNNNNRTG 4 cut(s) 779, 1017, 1523, 1686
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 6 cut(s) 522, 528, 837, 870, 1028, 1076
SseBI AGGCCT 1 cut(s) 1492
SsiI CCGC 4 cut(s) 131, 167, 257, 1622
SspMI CTAG 3 cut(s) 318, 405, 1611
StuI AGGCCT 1 cut(s) 1492
StyD4I CCNGG 3 cut(s) 67, 271, 1169
StyI CCWWGG 1 cut(s) 639
TaaI ACNGT 2 cut(s) 103, 1331
TaiI ACGT 1 cut(s) 342
TaqI TCGA 3 cut(s) 279, 417, 1639
TasI AATT 6 cut(s) 522, 528, 837, 870, 1028, 1076
TatI WGTACW 1 cut(s) 1285
TfiI GAWTC 3 cut(s) 89, 727, 1498
Tru1I TTAA 4 cut(s) 240, 933, 1445, 1532
Tru9I TTAA 4 cut(s) 240, 933, 1445, 1532
TscAI CASTG 7 cut(s) 488, 565, 705, 781, 820, 952, 1693
TseI GCWGC 3 cut(s) 1223, 1460, 1549
TspDTI ATGAA 8 cut(s) 237, 428, 534, 797, 876, 1021, 1193, 1641
TspGWI ACGGA 3 cut(s) 158, 413, 641
TspRI CASTG 7 cut(s) 488, 565, 705, 781, 820, 952, 1693
Vha464I CTTAAG 1 cut(s) 239
VpaK11BI GGWCC 3 cut(s) 55, 63, 232
XapI RAATTY 1 cut(s) 1076
XceI RCATGY 3 cut(s) 386, 614, 1099
XspI CTAG 3 cut(s) 318, 405, 1611
Zsp2I ATGCAT 3 cut(s) 369, 519, 1561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.