Rw6G037980

Tyrosine kinase, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001013
Physical Location & Seq
Forward (+)
62212980 .. 62220038
7059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G037980.1

Sequence Viewer

Length: 1716 bp
ATGGCGGAGGAAGACACGGAGAGTCGGTGTAGCATACCCGCGGGTTTTTCGCCGGCTTTGACCCGAAAGCAGAAACTGAAAGTGAATATCTACAATGAAATTCTGGTCCGGCTTAAGGACTCGGATGTCGCGGAGGCCCAACAGCCCGGTTTCGAAGATGAACTCTGGGCTCATTTCAATCGCCTCCCAACTAGGTACACCCCAGCCATCTTTCTCTACTCTCTAGCTACGTATGTATGCTTTGGATGTGAATGTGGAGAGGGCACAGATGTTCTCATGCATAAAAGACTATTGCATGTGGCACATGACCCTACCACTAGACCTGCAGTCGAAGTCCGTCTTGTGCAGGTTCATTTTTCTTCTAATTTAAGCTCATCCATAAAAGAAGATACTCAGTGTTCTGGTTTTGCTGGCAAACAGAGTCATCCACCACCAGCTTTTGGTTTGTCAGAAGACCTTGAACTTGCTCTTGAAGCTAAGAAGTTACAAGTTCAACATGGGAATACCGCTGTGAATGCCTTTTCAAGGCCGATGCATGAAATTACAATTTCAACTAATGACAAGCCCAAACTCCTCAGTGAGTTGACATCCTTATTATCTGACATCGGACTGAACATTCAAGAAGCACATGCTTTTTCTACAACAGATGGCTACTCCTTGGATGTCTTTGTCGTTGATGGTTGGGAATGTGAGGAAACTGAACAGCTTAGACAGGCACTGGCAAAAGAATTACTATGGAATGAGAATCCTTGCTTGGATTATAATGTCATCTCCCCTGTTCTGGGGCAAGACACTGGAATGAAGGTCATTGGTGATCATGTAAGCACATCCAGTGAAGAAAAAGATATCTGGGAAATTGAAGCAAGCCTATTGACATATGAAAGGAAAATTGCTTCTGGATCAAATGGTGATTTATATCAAGGTACTTTCTGTGGTCAAGATGTGGCCATTAAAGTTCTTAGCACTGAGCGCCTAAATGAAACCATGAGGGAGTTTACCCAAGAAGTCTCTATCATGAGAAAAGTTCGGCACAAGAATGTCGTTCAATTCATTGGGGCTTGCACTAAACCTCCTAACCTTTGCATAGTTACTGAATTTTTGTCTGGTGGAAGCATGTACGACTTTCTCCATAAGCAGAAGGGTTCTTTTTCACTTCAGTCCTTGCTCAAAGTTGCAGTTGATATTTCCAGGGGAATGAAATACTTGCACCAAAATAATATAATCCACAGGGACTTGAAAGCTGCTAATCTTTTGACGGATGGAAATGGAGTTGTGAAAGTAGCTGATTTTGGGATTGCTAGAGTACAGGCACAATCTGGTGTCATGACTGCAGAAACTGGCACCTACCGTTGGATGGCTCCAGAGGTCATAGAACATAAACCTTATGATCACAAAGCCGATGTTTTTAGCTTTGGGGTTATTCTGTGGGAGCTTCTTACAGGAAAGCTTCCCTATGAGAACTTAACTCCACTACAAGCAGCACTTGGCGTTGTCAAGAAGGGTCTGAGGCCTACGATTCCAAAGCACACTAACCCCATGTTAGTGGAGTTAATGGAGAGTTGCTGGCAGCAAGATGCATCGTTGAGACCTGAGTTCTCAGATATTGTAAATATGTTGCGACAAATGGCTAGGAGGGTTGCGGATGAAAGGGAGGATCGACGAAAGGGAAAATCAGCAAAAGTAGTACCCACTCTCAGGCATGGCAGTGCCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MS_channel Pkinase_Tyr

Protein Analysis

571

Amino Acids

63.78

Weight (kDa)

5.88

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 292 - 539 5.4e-74 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 293 - 537 1.4e-58 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 762
AasI GACNNNNNNGTC 1 cut(s) 125
Acc36I ACCTGC 2 cut(s) 331, 337
AccB1I GGYRCC 1 cut(s) 1340
AccB7I CCANNNNNTGG 1 cut(s) 440
AccII CGCG 2 cut(s) 41, 131
AciI CCGC 6 cut(s) 5, 39, 41, 131, 507, 1640
AclWI GGATC 2 cut(s) 907, 1662
AcoI YGGCCR 1 cut(s) 945
AcsI RAATTY 2 cut(s) 99, 1094
AcuI CTGAAG 1 cut(s) 1139
AfaI GTAC 5 cut(s) 197, 925, 1118, 1305, 1686
AfiI CCNNNNNNNGG 8 cut(s) 115, 440, 525, 781, 782, 1350, 1354, 1695
AflII CTTAAG 1 cut(s) 113
AhdI GACNNNNNGTC 1 cut(s) 326
AjnI CCWGG 1 cut(s) 1187
AjuI GAANNNNNNNTTGG 2 cut(s) 737, 769
Alw26I GTCTC 2 cut(s) 1012, 1579
AlwI GGATC 2 cut(s) 907, 1662
AlwNI CAGNNNCTG 3 cut(s) 76, 718, 1337
AoxI GGCC 4 cut(s) 135, 527, 945, 1508
ApeKI GCWGC 3 cut(s) 1241, 1478, 1567
ApoI RAATTY 2 cut(s) 99, 1094
AspLEI GCGC 1 cut(s) 972
AspS9I GGNCC 2 cut(s) 106, 136
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 2 cut(s) 824, 920
AsuII TTCGAA 1 cut(s) 153
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 266
BalI TGGCCA 1 cut(s) 947
BanI GGYRCC 1 cut(s) 1340
BanII GRGCYC 1 cut(s) 172
BbsI GAAGAC 2 cut(s) 18, 459
BbvI GCAGC 3 cut(s) 1228, 1490, 1579
BccI CCATC 5 cut(s) 215, 641, 671, 1253, 1348
BciT130I CCWGG 1 cut(s) 1189
BclI TGATCA 2 cut(s) 814, 1387
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 1012, 1579
BfaI CTAG 5 cut(s) 192, 224, 318, 1299, 1629
BfmI CTRYAG 2 cut(s) 324, 1329
BfoI RGCGCY 1 cut(s) 973
BfrI CTTAAG 1 cut(s) 113
BfuAI ACCTGC 2 cut(s) 331, 337
BisI GCNGC 3 cut(s) 1242, 1479, 1568
BlsI GCNGC 3 cut(s) 1243, 1480, 1569
Bme1390I CCNGG 2 cut(s) 147, 1189
Bme18I GGWCC 1 cut(s) 106
BmeRI GACNNNNNGTC 1 cut(s) 326
BmgT120I GGNCC 2 cut(s) 106, 136
BmiI GGNNCC 2 cut(s) 1342, 1359
BmrFI CCNGG 2 cut(s) 147, 1189
BmsI GCATC 3 cut(s) 522, 1564, 1586
BpiI GAAGAC 2 cut(s) 18, 459
BpmI CTGGAG 1 cut(s) 1344
Bpu14I TTCGAA 1 cut(s) 153
BpuMI CCSGG 1 cut(s) 147
BsaAI YACGTR 1 cut(s) 231
BsaBI GATNNNNATC 1 cut(s) 915
BsaI GGTCTC 1 cut(s) 1579
BsaJI CCNNGG 3 cut(s) 39, 657, 1188
BsaXI ACNNNNNCTCC 4 cut(s) 11, 41, 1054, 1084
Bsc4I CCNNNNNNNGG 8 cut(s) 115, 440, 525, 781, 782, 1350, 1354, 1695
Bse118I RCCGGY 1 cut(s) 52
Bse1I ACTGG 4 cut(s) 723, 799, 831, 1342
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 1 cut(s) 1189
BseDI CCNNGG 3 cut(s) 39, 657, 1188
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 8 cut(s) 115, 440, 525, 781, 782, 1350, 1354, 1695
BseMII CTCAG 7 cut(s) 407, 589, 957, 1496, 1581, 1611, 1708
BseNI ACTGG 4 cut(s) 723, 799, 831, 1342
BseRI GAGGAG 1 cut(s) 563
BseSI GKGCMC 1 cut(s) 266
BseXI GCAGC 3 cut(s) 1228, 1490, 1579
BseYI CCCAGC 1 cut(s) 202
BsgI GTGCAG 1 cut(s) 365
Bsh1236I CGCG 2 cut(s) 41, 131
BshFI GGCC 4 cut(s) 137, 529, 947, 1510
BshNI GGYRCC 1 cut(s) 1340
BsiSI CCGG 3 cut(s) 53, 109, 147
BslFI GGGAC 1 cut(s) 1244
BslI CCNNNNNNNGG 8 cut(s) 115, 440, 525, 781, 782, 1350, 1354, 1695
BsmAI GTCTC 2 cut(s) 1012, 1579
BsmFI GGGAC 1 cut(s) 1244
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 4 cut(s) 137, 529, 947, 1510
Bso31I GGTCTC 1 cut(s) 1579
Bsp119I TTCGAA 1 cut(s) 153
Bsp1286I GDGCHC 2 cut(s) 172, 266
Bsp143I GATC 4 cut(s) 814, 899, 1387, 1654
BspACI CCGC 6 cut(s) 5, 39, 41, 131, 507, 1640
BspANI GGCC 4 cut(s) 137, 529, 947, 1510
BspCNI CTCAG 7 cut(s) 406, 588, 958, 1497, 1582, 1610, 1707
BspFNI CGCG 2 cut(s) 41, 131
BspHI TCATGA 2 cut(s) 1014, 1323
BspLI GGNNCC 2 cut(s) 1342, 1359
BspMAI CTGCAG 2 cut(s) 328, 1333
BspMI ACCTGC 2 cut(s) 331, 337
BspPI GGATC 2 cut(s) 907, 1662
BspT104I TTCGAA 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 1340
BspTI CTTAAG 1 cut(s) 113
BspTNI GGTCTC 1 cut(s) 1579
BsrFI RCCGGY 1 cut(s) 52
BsrI ACTGG 4 cut(s) 723, 799, 831, 1342
BssAI RCCGGY 1 cut(s) 52
BssECI CCNNGG 3 cut(s) 39, 657, 1188
BssMI GATC 4 cut(s) 814, 899, 1387, 1654
BssT1I CCWWGG 1 cut(s) 657
Bst2UI CCWGG 1 cut(s) 1189
Bst4CI ACNGT 1 cut(s) 1349
BstAFI CTTAAG 1 cut(s) 113
BstBAI YACGTR 1 cut(s) 231
BstBI TTCGAA 1 cut(s) 153
BstC8I GCNNGC 5 cut(s) 54, 412, 865, 1060, 1565
BstDSI CCRYGG 1 cut(s) 39
BstENI CCTNNNNNAGG 1 cut(s) 523
BstFNI CGCG 2 cut(s) 41, 131
BstH2I RGCGCY 1 cut(s) 973
BstHHI GCGC 1 cut(s) 972
BstKTI GATC 4 cut(s) 817, 902, 1390, 1657
BstMAI GTCTC 2 cut(s) 1012, 1579
BstMBI GATC 4 cut(s) 814, 899, 1387, 1654
BstMWI GCNNNNNNNGC 3 cut(s) 473, 515, 969
BstNI CCWGG 1 cut(s) 1189
BstNSI RCATGY 3 cut(s) 299, 632, 1117
BstSCI CCNGG 2 cut(s) 145, 1187
BstSFI CTRYAG 2 cut(s) 324, 1329
BstSLI GKGCMC 1 cut(s) 266
BstSNI TACGTA 1 cut(s) 231
BstUI CGCG 2 cut(s) 41, 131
BstV1I GCAGC 3 cut(s) 1228, 1490, 1579
BstV2I GAAGAC 2 cut(s) 18, 459
BstXI CCANNNNNNTGG 1 cut(s) 1543
BsuRI GGCC 4 cut(s) 137, 529, 947, 1510
BtgI CCRYGG 1 cut(s) 39
BtsI GCAGTG 1 cut(s) 1711
BtsIMutI CAGTG 7 cut(s) 401, 583, 716, 792, 838, 963, 1711
BveI ACCTGC 2 cut(s) 331, 337
Cac8I GCNNGC 5 cut(s) 54, 412, 865, 1060, 1565
CaiI CAGNNNCTG 3 cut(s) 76, 718, 1337
CciI TCATGA 2 cut(s) 1014, 1323
CfoI GCGC 1 cut(s) 972
Cfr10I RCCGGY 1 cut(s) 52
Cfr13I GGNCC 2 cut(s) 106, 136
Cfr42I CCGCGG 1 cut(s) 42
Csp6I GTAC 5 cut(s) 196, 924, 1117, 1304, 1685
CviQI GTAC 5 cut(s) 196, 924, 1117, 1304, 1685
DpnI GATC 4 cut(s) 816, 901, 1389, 1656
DpnII GATC 4 cut(s) 814, 899, 1387, 1654
DrdI GACNNNNNNGTC 1 cut(s) 125
DriI GACNNNNNGTC 1 cut(s) 326
DseDI GACNNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 1 cut(s) 945
Eam1105I GACNNNNNGTC 1 cut(s) 326
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 657
Eco147I AGGCCT 1 cut(s) 1510
Eco24I GRGCYC 1 cut(s) 172
Eco31I GGTCTC 1 cut(s) 1579
Eco32I GATATC 1 cut(s) 847
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 1139
EcoNI CCTNNNNNAGG 1 cut(s) 523
EcoRII CCWGG 1 cut(s) 1187
EcoRV GATATC 1 cut(s) 847
EcoT14I CCWWGG 1 cut(s) 657
EcoT22I ATGCAT 3 cut(s) 282, 537, 1579
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 657
FalI AAGNNNNNCTT 4 cut(s) 324, 356, 1467, 1499
FaqI GGGAC 1 cut(s) 1244
FauI CCCGC 2 cut(s) 34, 46
FauNDI CATATG 1 cut(s) 877
FbaI TGATCA 2 cut(s) 814, 1387
Fnu4HI GCNGC 3 cut(s) 1242, 1479, 1568
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 3 cut(s) 1242, 1479, 1568
FspBI CTAG 5 cut(s) 192, 224, 318, 1299, 1629
GlaI GCGC 1 cut(s) 971
GluI GCNGC 3 cut(s) 1242, 1479, 1568
GsaI CCCAGC 1 cut(s) 206
GsuI CTGGAG 1 cut(s) 1344
HaeII RGCGCY 1 cut(s) 973
HaeIII GGCC 4 cut(s) 137, 529, 947, 1510
HapII CCGG 3 cut(s) 53, 109, 147
HhaI GCGC 1 cut(s) 972
Hin6I GCGC 1 cut(s) 970
HinP1I GCGC 1 cut(s) 970
HincII GTYRAC 1 cut(s) 585
HindII GTYRAC 1 cut(s) 585
HindIII AAGCTT 1 cut(s) 1445
HinfI GANTC 5 cut(s) 22, 119, 421, 745, 1516
HpaII CCGG 3 cut(s) 53, 109, 147
HphI GGTGA 2 cut(s) 824, 920
Hpy166II GTNNAC 3 cut(s) 198, 585, 996
Hpy188I TCNGA 6 cut(s) 124, 451, 601, 608, 1506, 1600
Hpy188III TCNNGA 8 cut(s) 470, 620, 897, 938, 1015, 1324, 1361, 1495
Hpy8I GTNNAC 3 cut(s) 198, 585, 996
Hpy99I CGWCG 1 cut(s) 1662
HpyAV CCTTC 3 cut(s) 796, 1132, 1492
HpyCH4III ACNGT 1 cut(s) 1349
HpyCH4IV ACGT 1 cut(s) 230
HpyF10VI GCNNNNNNNGC 3 cut(s) 473, 515, 969
HpySE526I ACGT 1 cut(s) 230
HspAI GCGC 1 cut(s) 970
KroI GCCGGC 1 cut(s) 52
KroNI GCCGGC 1 cut(s) 54
Ksp22I TGATCA 2 cut(s) 814, 1387
KspI CCGCGG 1 cut(s) 42
Kzo9I GATC 4 cut(s) 814, 899, 1387, 1654
LmnI GCTCC 2 cut(s) 1363, 1429
Lsp1109I GCAGC 3 cut(s) 1228, 1490, 1579
LweI GCATC 3 cut(s) 522, 1564, 1586
MaeI CTAG 5 cut(s) 192, 224, 318, 1299, 1629
MaeII ACGT 1 cut(s) 230
MaeIII GTNAC 2 cut(s) 483, 1087
MalI GATC 4 cut(s) 816, 901, 1389, 1656
MboI GATC 4 cut(s) 814, 899, 1387, 1654
MboII GAAGA 6 cut(s) 23, 167, 351, 398, 464, 848
MhlI GDGCHC 2 cut(s) 172, 266
MlsI TGGCCA 1 cut(s) 947
MluCI AATT 9 cut(s) 99, 364, 540, 546, 728, 855, 888, 1046, 1094
MluNI TGGCCA 1 cut(s) 947
MlyI GAGTC 3 cut(s) 31, 113, 430
MmeI TCCRAC 1 cut(s) 1331
Mox20I TGGCCA 1 cut(s) 947
Mph1103I ATGCAT 3 cut(s) 282, 537, 1579
MroNI GCCGGC 1 cut(s) 52
MscI TGGCCA 1 cut(s) 947
MseI TTAA 5 cut(s) 114, 368, 951, 1463, 1550
MslI CAYNNNNRTG 4 cut(s) 797, 1035, 1541, 1704
Msp20I TGGCCA 1 cut(s) 947
MspA1I CMGCKG 2 cut(s) 41, 509
MspCI CTTAAG 1 cut(s) 113
MspI CCGG 3 cut(s) 53, 109, 147
MspR9I CCNGG 2 cut(s) 147, 1189
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 1 cut(s) 1189
MvnI CGCG 2 cut(s) 41, 131
MwoI GCNNNNNNNGC 3 cut(s) 473, 515, 969
NaeI GCCGGC 1 cut(s) 54
NciI CCSGG 1 cut(s) 147
NdeI CATATG 1 cut(s) 877
NdeII GATC 4 cut(s) 814, 899, 1387, 1654
NgoMIV GCCGGC 1 cut(s) 52
NlaIV GGNNCC 2 cut(s) 1342, 1359
NsiI ATGCAT 3 cut(s) 282, 537, 1579
NspI RCATGY 3 cut(s) 299, 632, 1117
NspV TTCGAA 1 cut(s) 153
PagI TCATGA 2 cut(s) 1014, 1323
PceI AGGCCT 1 cut(s) 1510
PctI GAATGC 1 cut(s) 520
PdiI GCCGGC 1 cut(s) 54
PfeI GAWTC 2 cut(s) 745, 1516
PflMI CCANNNNNTGG 1 cut(s) 440
PkrI GCNGC 3 cut(s) 1243, 1480, 1569
PleI GAGTC 3 cut(s) 30, 113, 429
PpsI GAGTC 3 cut(s) 30, 113, 429
Ppu21I YACGTR 1 cut(s) 231
PsiI TTATAA 1 cut(s) 762
Psp6I CCWGG 1 cut(s) 1187
PspFI CCCAGC 1 cut(s) 202
PspGI CCWGG 1 cut(s) 1187
PspN4I GGNNCC 2 cut(s) 1342, 1359
PspPI GGNCC 2 cut(s) 106, 136
PstI CTGCAG 2 cut(s) 328, 1333
PstNI CAGNNNCTG 3 cut(s) 76, 718, 1337
RsaI GTAC 5 cut(s) 197, 925, 1118, 1305, 1686
RsaNI GTAC 5 cut(s) 196, 924, 1117, 1304, 1685
RseI CAYNNNNRTG 4 cut(s) 797, 1035, 1541, 1704
SacII CCGCGG 1 cut(s) 42
SaqAI TTAA 5 cut(s) 114, 368, 951, 1463, 1550
SatI GCNGC 3 cut(s) 1242, 1479, 1568
Sau3AI GATC 4 cut(s) 814, 899, 1387, 1654
Sau96I GGNCC 2 cut(s) 106, 136
SchI GAGTC 3 cut(s) 31, 113, 430
ScrFI CCNGG 2 cut(s) 147, 1189
SduI GDGCHC 2 cut(s) 172, 266
SfaNI GCATC 3 cut(s) 522, 1564, 1586
SfcI CTRYAG 2 cut(s) 324, 1329
Sfr303I CCGCGG 1 cut(s) 42
SfuI TTCGAA 1 cut(s) 153
SgrBI CCGCGG 1 cut(s) 42
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 4 cut(s) 797, 1035, 1541, 1704
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
SnaBI TACGTA 1 cut(s) 231
Sse9I AATT 9 cut(s) 99, 364, 540, 546, 728, 855, 888, 1046, 1094
SseBI AGGCCT 1 cut(s) 1510
SsiI CCGC 6 cut(s) 5, 39, 41, 131, 507, 1640
SspMI CTAG 5 cut(s) 192, 224, 318, 1299, 1629
StuI AGGCCT 1 cut(s) 1510
StyD4I CCNGG 2 cut(s) 145, 1187
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 1 cut(s) 1349
TaiI ACGT 1 cut(s) 233
TaqI TCGA 3 cut(s) 153, 330, 1657
TasI AATT 9 cut(s) 99, 364, 540, 546, 728, 855, 888, 1046, 1094
TatI WGTACW 1 cut(s) 1303
TfiI GAWTC 2 cut(s) 745, 1516
Tru1I TTAA 5 cut(s) 114, 368, 951, 1463, 1550
Tru9I TTAA 5 cut(s) 114, 368, 951, 1463, 1550
TscAI CASTG 7 cut(s) 401, 583, 723, 799, 838, 970, 1711
TseI GCWGC 3 cut(s) 1241, 1478, 1567
TspGWI ACGGA 3 cut(s) 32, 326, 1271
TspRI CASTG 7 cut(s) 401, 583, 723, 799, 838, 970, 1711
Van91I CCANNNNNTGG 1 cut(s) 440
Vha464I CTTAAG 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 106
XagI CCTNNNNNAGG 1 cut(s) 523
XapI RAATTY 2 cut(s) 99, 1094
XceI RCATGY 3 cut(s) 299, 632, 1117
XspI CTAG 5 cut(s) 192, 224, 318, 1299, 1629
Zsp2I ATGCAT 3 cut(s) 282, 537, 1579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.