Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001013

Physical Location & Seq

Genomic Coordinates Reverse (-)

21026534 .. 21033410

Gene Structure 6877 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1130000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1299 bp

ATGAGTGCAGCAGATACTATGAATACAGATCCTCCGGGTAAAGATGCTGCCCAAAGTTCAAACATATCTACCAGACAAAGTATACATCCCCCTCCAGCGTTTGGCTCATCTCCTAACCTCGAAGCCCTTGCACTGGAGGCAAATAAATCTGAAGATCATGATGCTTTGACTTCCTTGCTTGCGGAGATTGGGCTGAACATCCAAGAAGCACATGCTTTTTCCACATTAGATGGTTACTCCTTGGATGTCTTTGTTGTTGATGGATGGCGTTACGAGGAGACAGATAAGCTTAAGATCGCAATACAAAAGGAAGTTTTAAAGATTGAGAGGCCGTGGCCGCCAACTCATCGATCATCATCTTTTGGTAGCGAGCATGATCAAGTAGTGATCAAAACTGAACCTGATCATCTGGAAATACCTAATGATGGGACTGATGTATGGGAACTTGATCCTAGACAATTGAAGTTTGGGAATAAAGTTGCATCTGGGTCCTGTGGAGATTTATATAAAGGTACATATTGTACTCAAGAAGTTGCCATTAAAGTCCTCAAGCCCGAGTGTGTGAATCCAGATATGCTGAAAGATTTTGCCCAGGAAGTCTACATTATGAGGAAAGTTCGACACAAGAATGTTGTACAATTCATTGGTGCGTGTACCAAGCATCCAAGCTTATGCATTGTAACAGAATATATGTCCGGTGGAAGTGTGTATGACTACTTGCATAAACAAAAGGGAGTTTTTAAGCTTCCATCCTTGCTCAAGGTCGCAATTGATGTTTCCAAGGGAATGACCTACTTGCATCAAAATAACATAATCCACAGGGATTTAAAAGCCGCCAATCTCTTGATGGATGAAAATGAAGTTGTTAAGGTTGCTGATTTCGGGGTTGCTAGAGTAAATTCTCATTCTGGAGTAATGACATCAGAAACTGGGACTTATAGGTGGATGGCTCCAGAGGTCATAGAACACAAGCCATATGATCACAAGGCGGATGTTTTTAGCTTTGGACTTGTGTTATGGGAGTTACTGACTGGAAAGCTTCCATATGAACACTTGACCCCATTACAAGCAGCCGTCGGAGTTGCCCAAAAGGGCTTACGGCCGACCATCCCAAAGAATACTCCTCCCAAGCTTGCCGAGCTGCTCGAAAAATGCTGGCAGCAAGATCCAGCAACAAGACCTGATTTCTCTGAAATCATAGAGAATCTGCAGACACTAGCCGAAGAGGTTGGTGATGGAGTGGAACGACACAAATCATCGGGAGGATTTCTGTCAGTCCTGAGACGGGGTCACCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko01521 ko01522 ko04010 ko04012 ko04013 ko04015 ko04024 ko04062 ko04068 ko04150 ko04214 ko04270 ko04320 ko04510 ko04650 ko04720 ko04722 ko04726 ko04730 ko04810 ko04910 ko04914 ko04934 ko05034 ko05160 ko05200 ko05205 ko05210 ko05211 ko05212 ko05213 ko05214 ko05215 ko05216 ko05218 ko05219 ko05220 ko05221 ko05223 ko05224 ko05225 ko05226 map01521 map01522 map04010 map04012 map04013 map04015 map04024 map04062 map04068 map04150 map04214 map04270 map04320 map04510 map04650 map04720 map04722 map04726 map04730 map04810 map04910 map04914 map04934 map05034 map05160 map05200 map05205 map05210 map05211 map05212 map05213 map05214 map05215 map05216 map05218 map05219 map05220 map05221 map05223 map05224 map05225 map05226

Pfam Domains

Protein Families

ACT Pkinase_Tyr

Protein Analysis

433

Amino Acids

48.04

Weight (kDa)

5.84

Isoelectric Point (pI)

46.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	154 - 403	2.6e-75	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	157 - 401	4.9e-60	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38470 AT4G38470 AT4G38470
fragaria_vesca	FvH4_2g15800 FvH4_2g15800 FvH4_2g15800 FvH4_2g15800 FvH4_2g15800 FvH4_2g15800 FvH4_2g15800 FvH4_2g15800 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880 FvH4_2g37880
malus_domestica	MD05G1126800.v1.1 MD10G1130000.v1.1 MD10G1130300.v1.1 MD15G1006000.v1.1
prunus_persica	Prupe.1G358800_v2.0.a1 Prupe.8G170600_v2.0.a1 Prupe.8G170600_v2.0.a1 Prupe.8G170600_v2.0.a1
pyrus_communis	pycom05g12270 pycom15g00460
rosa_chinensis	RchiOBHm_Chr6g0279141 RchiOBHm_Chr6g0303741
rosa_laevigata	RLG00000011050 RLG00000013163
rosa_multiflora	Rmu_co8370501.1_g000001 Rmu_sc0006400.1_g000001 Rmu_sc0009053.1_g000001 Rmu_sc0012878.1_g000007 Rmu_sc0014203.1_g000019
rosa_roxburghii	Rroxscaffold_7G00164440 Rroxscaffold_7G00189090
rosa_rugosa	Rorug06G0122600 Rorug06G0122700 Rorug06G0122800 Rorug06G0122900 Rorug06G0324400
rosa_samantha	Rh6AG231400 Rh6AG438700 Rh6BG236300 Rh6BG464400 Rh6CG238400 Rh6CG451000 Rh6DG229900 Rh6DG437800
rosa_wichuraiana	Rw6G020260 Rw6G037980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	101
AccI	GTMKAC	2 cut(s)	82, 600
AciI	CCGC	4 cut(s)	182, 338, 834, 989
AclWI	GGATC	3 cut(s)	23, 443, 1160
AcoI	YGGCCR	2 cut(s)	335, 1100
AcsI	RAATTY	1 cut(s)	898
AcuI	CTGAAG	1 cut(s)	171
AfaI	GTAC	4 cut(s)	514, 523, 636, 655
AfiI	CCNNNNNNNGG	4 cut(s)	101, 133, 425, 1285
AflII	CTTAAG	1 cut(s)	290
AgsI	TTSAA	2 cut(s)	60, 463
AjnI	CCWGG	1 cut(s)	591
AluBI	AGCT	7 cut(s)	289, 669, 745, 1002, 1039, 1132, 1141
AluI	AGCT	7 cut(s)	289, 669, 745, 1002, 1039, 1132, 1141
Alw26I	GTCTC	2 cut(s)	272, 1276
AlwI	GGATC	3 cut(s)	23, 443, 1160
AlwNI	CAGNNNCTG	1 cut(s)	929
Ama87I	CYCGRG	1 cut(s)	554
AoxI	GGCC	3 cut(s)	329, 335, 1100
ApeKI	GCWGC	5 cut(s)	8, 47, 1070, 1141, 1159
ApoI	RAATTY	1 cut(s)	898
AspS9I	GGNCC	1 cut(s)	489
AsuC2I	CCSGG	1 cut(s)	36
AsuHPI	GGTGA	2 cut(s)	1244, 1283
AvaI	CYCGRG	1 cut(s)	554
AvaII	GGWCC	1 cut(s)	489
BbvI	GCAGC	5 cut(s)	20, 34, 1082, 1128, 1171
BccI	CCATC	9 cut(s)	224, 254, 258, 419, 757, 841, 940, 1115, 1229
BceAI	ACGGC	3 cut(s)	316, 1058, 1115
BcgI	CGANNNNNNTGC	2 cut(s)	110, 144
BciT130I	CCWGG	1 cut(s)	593
BclI	TGATCA	4 cut(s)	376, 387, 403, 979
BcnI	CCSGG	1 cut(s)	36
BcoDI	GTCTC	2 cut(s)	272, 1276
BfaI	CTAG	3 cut(s)	453, 891, 1217
BfmI	CTRYAG	1 cut(s)	1208
BfrI	CTTAAG	1 cut(s)	290
BisI	GCNGC	7 cut(s)	9, 48, 338, 834, 1071, 1142, 1160
BlsI	GCNGC	7 cut(s)	10, 49, 339, 835, 1072, 1143, 1161
Bme1390I	CCNGG	2 cut(s)	36, 593
Bme18I	GGWCC	1 cut(s)	489
BmeT110I	CYCGRG	1 cut(s)	554
BmgT120I	GGNCC	1 cut(s)	489
BmiI	GGNNCC	2 cut(s)	490, 951
BmrFI	CCNGG	2 cut(s)	36, 593
BmrI	ACTGGG	1 cut(s)	939
BmsI	GCATC	5 cut(s)	34, 151, 491, 670, 808
BmuI	ACTGGG	1 cut(s)	939
BpmI	CTGGAG	4 cut(s)	78, 155, 930, 936
BpuEI	CTTGAG	3 cut(s)	510, 533, 743
BpuMI	CCSGG	1 cut(s)	36
Bsa29I	ATCGAT	1 cut(s)	349
BsaBI	GATNNNNATC	1 cut(s)	355
BsaJI	CCNNGG	4 cut(s)	240, 332, 591, 780
BsaWI	WCCGGW	1 cut(s)	695
BsaXI	ACNNNNNCTCC	2 cut(s)	16, 46
Bsc4I	CCNNNNNNNGG	4 cut(s)	101, 133, 425, 1285
Bse1I	ACTGG	3 cut(s)	138, 934, 1036
Bse8I	GATNNNNATC	1 cut(s)	355
BseBI	CCWGG	1 cut(s)	593
BseCI	ATCGAT	1 cut(s)	349
BseDI	CCNNGG	4 cut(s)	240, 332, 591, 780
BseJI	GATNNNNATC	1 cut(s)	355
BseLI	CCNNNNNNNGG	4 cut(s)	101, 133, 425, 1285
BseMII	CTCAG	1 cut(s)	1271
BseNI	ACTGG	3 cut(s)	138, 934, 1036
BseRI	GAGGAG	2 cut(s)	290, 1113
BseX3I	CGGCCG	1 cut(s)	1100
BseXI	GCAGC	5 cut(s)	20, 34, 1082, 1128, 1171
BsgI	GTGCAG	1 cut(s)	27
Bsh1285I	CGRYCG	1 cut(s)	1103
BshFI	GGCC	3 cut(s)	331, 337, 1102
BshVI	ATCGAT	1 cut(s)	349
BsiEI	CGRYCG	1 cut(s)	1103
BsiHKCI	CYCGRG	1 cut(s)	554
BsiSI	CCGG	2 cut(s)	35, 696
BslFI	GGGAC	2 cut(s)	442, 946
BslI	CCNNNNNNNGG	4 cut(s)	101, 133, 425, 1285
BsmAI	GTCTC	2 cut(s)	272, 1276
BsmBI	CGTCTC	1 cut(s)	1276
BsmFI	GGGAC	2 cut(s)	442, 946
BsnI	GGCC	3 cut(s)	331, 337, 1102
BsoBI	CYCGRG	1 cut(s)	554
Bsp1407I	TGTACA	1 cut(s)	634
BspACI	CCGC	4 cut(s)	182, 338, 834, 989
BspANI	GGCC	3 cut(s)	331, 337, 1102
BspCNI	CTCAG	1 cut(s)	1272
BspDI	ATCGAT	1 cut(s)	349
BspHI	TCATGA	1 cut(s)	157
BspLI	GGNNCC	2 cut(s)	490, 951
BspMAI	CTGCAG	1 cut(s)	1212
BspPI	GGATC	3 cut(s)	23, 443, 1160
BspTI	CTTAAG	1 cut(s)	290
BsrGI	TGTACA	1 cut(s)	634
BsrI	ACTGG	3 cut(s)	138, 934, 1036
BssECI	CCNNGG	4 cut(s)	240, 332, 591, 780
BssNAI	GTATAC	1 cut(s)	83
BssT1I	CCWWGG	2 cut(s)	240, 780
Bst1107I	GTATAC	1 cut(s)	83
Bst2UI	CCWGG	1 cut(s)	593
Bst6I	CTCTTC	1 cut(s)	1218
BstAFI	CTTAAG	1 cut(s)	290
BstAUI	TGTACA	1 cut(s)	634
BstC8I	GCNNGC	4 cut(s)	180, 371, 1134, 1157
BstDEI	CTNAG	1 cut(s)	1280
BstDSI	CCRYGG	1 cut(s)	332
BstEII	GGTNACC	1 cut(s)	1289
BstMAI	GTCTC	2 cut(s)	272, 1276
BstMCI	CGRYCG	1 cut(s)	1103
BstMWI	GCNNNNNNNGC	3 cut(s)	137, 337, 1138
BstNI	CCWGG	1 cut(s)	593
BstNSI	RCATGY	1 cut(s)	215
BstPI	GGTNACC	1 cut(s)	1289
BstSCI	CCNGG	2 cut(s)	34, 591
BstSFI	CTRYAG	1 cut(s)	1208
BstV1I	GCAGC	5 cut(s)	20, 34, 1082, 1128, 1171
BstX2I	RGATCY	2 cut(s)	28, 1165
BstYI	RGATCY	2 cut(s)	28, 1165
BstZ17I	GTATAC	1 cut(s)	83
BstZI	CGGCCG	1 cut(s)	1100
Bsu15I	ATCGAT	1 cut(s)	349
BsuRI	GGCC	3 cut(s)	331, 337, 1102
BsuTUI	ATCGAT	1 cut(s)	349
BtgI	CCRYGG	1 cut(s)	332
BtsIMutI	CAGTG	1 cut(s)	131
Cac8I	GCNNGC	4 cut(s)	180, 371, 1134, 1157
CaiI	CAGNNNCTG	1 cut(s)	929
CciI	TCATGA	1 cut(s)	157
Cfr13I	GGNCC	1 cut(s)	489
ClaI	ATCGAT	1 cut(s)	349
Csp6I	GTAC	4 cut(s)	513, 522, 635, 654
CviAII	CATG	3 cut(s)	158, 212, 374
CviQI	GTAC	4 cut(s)	513, 522, 635, 654
DdeI	CTNAG	1 cut(s)	1280
DraI	TTTAAA	2 cut(s)	318, 828
EaeI	YGGCCR	2 cut(s)	335, 1100
EagI	CGGCCG	1 cut(s)	1100
Eam1104I	CTCTTC	1 cut(s)	1218
EarI	CTCTTC	1 cut(s)	1218
EciI	GGCGGA	1 cut(s)	1004
EclXI	CGGCCG	1 cut(s)	1100
Eco130I	CCWWGG	2 cut(s)	240, 780
Eco47I	GGWCC	1 cut(s)	489
Eco52I	CGGCCG	1 cut(s)	1100
Eco57I	CTGAAG	1 cut(s)	171
Eco88I	CYCGRG	1 cut(s)	554
Eco91I	GGTNACC	1 cut(s)	1289
EcoO109I	RGGNCCY	1 cut(s)	489
EcoO65I	GGTNACC	1 cut(s)	1289
EcoRII	CCWGG	1 cut(s)	591
EcoT14I	CCWWGG	2 cut(s)	240, 780
EcoT22I	ATGCAT	1 cut(s)	677
ErhI	CCWWGG	2 cut(s)	240, 780
Esp3I	CGTCTC	1 cut(s)	1276
FaeI	CATG	3 cut(s)	161, 215, 377
FaqI	GGGAC	2 cut(s)	442, 946
FatI	CATG	3 cut(s)	157, 211, 373
FauNDI	CATATG	2 cut(s)	976, 1045
FbaI	TGATCA	4 cut(s)	376, 387, 403, 979
FblI	GTMKAC	2 cut(s)	82, 600
Fnu4HI	GCNGC	7 cut(s)	9, 48, 338, 834, 1071, 1142, 1160
Fsp4HI	GCNGC	7 cut(s)	9, 48, 338, 834, 1071, 1142, 1160
FspBI	CTAG	3 cut(s)	453, 891, 1217
GluI	GCNGC	7 cut(s)	9, 48, 338, 834, 1071, 1142, 1160
GsuI	CTGGAG	4 cut(s)	78, 155, 930, 936
HaeIII	GGCC	3 cut(s)	331, 337, 1102
HapII	CCGG	2 cut(s)	35, 696
Hin1II	CATG	3 cut(s)	161, 215, 377
HindIII	AAGCTT	5 cut(s)	287, 667, 743, 1037, 1130
HinfI	GANTC	2 cut(s)	565, 1204
HpaII	CCGG	2 cut(s)	35, 696
HphI	GGTGA	2 cut(s)	1244, 1283
Hpy166II	GTNNAC	3 cut(s)	83, 601, 654
Hpy188I	TCNGA	4 cut(s)	151, 925, 1079, 1192
Hpy188III	TCNNGA	9 cut(s)	158, 410, 527, 569, 844, 909, 953, 1260, 1279
Hpy8I	GTNNAC	3 cut(s)	83, 601, 654
Hpy99I	CGWCG	1 cut(s)	1079
HpyCH4V	TGCA	7 cut(s)	8, 131, 482, 675, 721, 799, 1210
HpyF10VI	GCNNNNNNNGC	3 cut(s)	137, 337, 1138
HpyF3I	CTNAG	1 cut(s)	1280
Hsp92II	CATG	3 cut(s)	161, 215, 377
Ksp22I	TGATCA	4 cut(s)	376, 387, 403, 979
LmnI	GCTCC	1 cut(s)	955
Lsp1109I	GCAGC	5 cut(s)	20, 34, 1082, 1128, 1171
LweI	GCATC	5 cut(s)	34, 151, 491, 670, 808
MaeI	CTAG	3 cut(s)	453, 891, 1217
MaeIII	GTNAC	5 cut(s)	233, 269, 679, 1023, 1289
MboII	GAAGA	2 cut(s)	164, 1235
MfeI	CAATTG	2 cut(s)	458, 768
MflI	RGATCY	2 cut(s)	28, 1165
MluCI	AATT	4 cut(s)	458, 638, 768, 898
MmeI	TCCRAC	1 cut(s)	1057
Mph1103I	ATGCAT	1 cut(s)	677
MseI	TTAA	7 cut(s)	291, 317, 540, 741, 827, 867, 1297
MslI	CAYNNNNRTG	1 cut(s)	627
MspCI	CTTAAG	1 cut(s)	290
MspI	CCGG	2 cut(s)	35, 696
MspR9I	CCNGG	2 cut(s)	36, 593
MunI	CAATTG	2 cut(s)	458, 768
MvaI	CCWGG	1 cut(s)	593
MwoI	GCNNNNNNNGC	3 cut(s)	137, 337, 1138
NciI	CCSGG	1 cut(s)	36
NdeI	CATATG	2 cut(s)	976, 1045
NlaIII	CATG	3 cut(s)	161, 215, 377
NlaIV	GGNNCC	2 cut(s)	490, 951
NmeAIII	GCCGAG	1 cut(s)	1162
NmuCI	GTSAC	1 cut(s)	1289
NsiI	ATGCAT	1 cut(s)	677
NspI	RCATGY	1 cut(s)	215
PagI	TCATGA	1 cut(s)	157
PfeI	GAWTC	2 cut(s)	565, 1204
PflFI	GACNNNGTC	1 cut(s)	1287
PflMI	CCANNNNNTGG	1 cut(s)	101
PkrI	GCNGC	7 cut(s)	10, 49, 339, 835, 1072, 1143, 1161
PpuMI	RGGWCCY	1 cut(s)	489
Psp5II	RGGWCCY	1 cut(s)	489
Psp6I	CCWGG	1 cut(s)	591
PspEI	GGTNACC	1 cut(s)	1289
PspGI	CCWGG	1 cut(s)	591
PspN4I	GGNNCC	2 cut(s)	490, 951
PspPI	GGNCC	1 cut(s)	489
PspPPI	RGGWCCY	1 cut(s)	489
PstI	CTGCAG	1 cut(s)	1212
PstNI	CAGNNNCTG	1 cut(s)	929
PsuI	RGATCY	2 cut(s)	28, 1165
PsyI	GACNNNGTC	1 cut(s)	1287
RsaI	GTAC	4 cut(s)	514, 523, 636, 655
RsaNI	GTAC	4 cut(s)	513, 522, 635, 654
RseI	CAYNNNNRTG	1 cut(s)	627
SaqAI	TTAA	7 cut(s)	291, 317, 540, 741, 827, 867, 1297
SatI	GCNGC	7 cut(s)	9, 48, 338, 834, 1071, 1142, 1160
Sau96I	GGNCC	1 cut(s)	489
ScrFI	CCNGG	2 cut(s)	36, 593
SfaNI	GCATC	5 cut(s)	34, 151, 491, 670, 808
SfcI	CTRYAG	1 cut(s)	1208
SinI	GGWCC	1 cut(s)	489
SmiMI	CAYNNNNRTG	1 cut(s)	627
SmlI	CTYRAG	4 cut(s)	290, 525, 548, 758
SmoI	CTYRAG	4 cut(s)	290, 525, 548, 758
Sse9I	AATT	4 cut(s)	458, 638, 768, 898
SsiI	CCGC	4 cut(s)	182, 338, 834, 989
SspMI	CTAG	3 cut(s)	453, 891, 1217
StyD4I	CCNGG	2 cut(s)	34, 591
StyI	CCWWGG	2 cut(s)	240, 780
TaqI	TCGA	4 cut(s)	120, 349, 619, 1146
TasI	AATT	4 cut(s)	458, 638, 768, 898
TatI	WGTACW	2 cut(s)	521, 634
TauI	GCSGC	2 cut(s)	340, 836
TfiI	GAWTC	2 cut(s)	565, 1204
Tru1I	TTAA	7 cut(s)	291, 317, 540, 741, 827, 867, 1297
Tru9I	TTAA	7 cut(s)	291, 317, 540, 741, 827, 867, 1297
TscAI	CASTG	1 cut(s)	138
TseFI	GTSAC	1 cut(s)	1289
TseI	GCWGC	5 cut(s)	8, 47, 1070, 1141, 1159
Tsp45I	GTSAC	1 cut(s)	1289
TspDTI	ATGAA	5 cut(s)	35, 631, 867, 873, 1062
TspRI	CASTG	1 cut(s)	138
Tth111I	GACNNNGTC	1 cut(s)	1287
Van91I	CCANNNNNTGG	1 cut(s)	101
Vha464I	CTTAAG	1 cut(s)	290
VpaK11BI	GGWCC	1 cut(s)	489
XapI	RAATTY	1 cut(s)	898
XceI	RCATGY	1 cut(s)	215
XcmI	CCANNNNNNNNNTGG	1 cut(s)	844
XmiI	GTMKAC	2 cut(s)	82, 600
XspI	CTAG	3 cut(s)	453, 891, 1217
Zsp2I	ATGCAT	1 cut(s)	677

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.