Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002103

Physical Location & Seq

Genomic Coordinates Reverse (-)

34588303 .. 34592331

Gene Structure 4029 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1214900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1230 bp

ATGGGTTGTTTTTCCTTCTTAAGCGGTAGAATTGTGAACAAATCAAGAAAACCCGGTTCTGATATTGATGAAGAAGTTTCTGGCATTCACAATGTTAAACTATTCACTTACAAAGAGTTGAGAATCGCAACTGACGATTTTAGTCCAGCCAATAAAATCGGGGAAGGTGGTTTTGGTTCTGTCTATAAGGGCCAACTTAAAGACAGAAAATTTGCTGCTATAAAAGTTCTTTCAGCTGAATCAAGACAAGGGGTGAAGGAGTTTTTGACTGAGATTGACGTGATCTCAAAAATAAAGCATGAAAATCTAGTTGAGCTCTTTGGCTGTTGTGTTGAAGGAAACCAAAGAATTCTGGTCTACAACTACCTCGAGAATAATAGCCTTGAACAAAATCTTATTGGTGGAAGCAGTAATCTCCAGTTTAGTTGGCGAACTAGGCGTGAAATATGCAATGGGATTGCATGTGGGCTTGCCTTCCTTCATGAGGAAGTACGTCCACATATTATTCATAGAGATATCAAAGCAAGCAATATTCTCCTCGATAAAGACCTAATGCCTAAAATTTCAGATTTTGGTCTTGCAAAACTTATCCCTCCCAACATGACCCATGTTAGCACACGTGTGGCAGGAACAATAGGTTATTTGGCACCAGAATATGCAATACGCGGTCAATTGACAAGGAAAGCAGATATTTATAGCTTTGGAGTGCTCCTAATGGAAATAGTCAGTGGCAGATGTAATACAAATACACGACTACCAATTGACGAACAGTATCTGCTTGAAAGGACATGGAAACTCTACGAACGGAGGGAGCTTGTTGGGCTGGTAGACACATCACTCGACGGTGATTTTGACGCTGAGGAGGCTTGTAGGTTTCTAAAGATCGGTCTCCTCTGCACCCAAGACTCTCCGAAGCTCAGGCCATCCATGTCAACCGTGGTCAAGATGCTAAAAGGCGAGAAGGTTGTGGACGATAAGATAACAAAGCCAGGCTTAATATCCGATTTCATGGATCTCGGAGTACGAGGCCCTCATGGCACCAAGCCTGGTGCAGAGACGACGACATCTTCCTATAATGCATCCGCTGGCTCAGGCTCAGACAACCAGTACAATTCAACCTTCTCATTAGCAACCTCAGCTGCTGCTACCACCACCACCACCTTCATTTCGAACTTGTCATCTGAAACCTCAGCCGCTAATACTAAGAACTTCAGCATCCGCACCATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

410

Amino Acids

45.14

Weight (kDa)

8.02

Isoelectric Point (pI)

29.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	50 - 314	5.9e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	51 - 316	1.1e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002103)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16670
fragaria_vesca	FvH4_3g16070 FvH4_3g16070 FvH4_3g16070 FvH4_3g16070 FvH4_3g18580
malus_domestica	MD03G1240900.v1.1 MD05G1214900.v1.1 MD10G1198000.v1.1 MD11G1260300.v1.1
prunus_persica	Prupe.4G143900_v2.0.a1 Prupe.4G143900_v2.0.a1 Prupe.4G168000_v2.0.a1 Prupe.4G168000_v2.0.a1 Prupe.4G168000_v2.0.a1
pyrus_communis	pycom03g18740 pycom05g19800 pycom10g17070 pycom11g22980
rosa_chinensis	RchiOBHm_Chr5g0027041 RchiOBHm_Chr5g0031391
rosa_laevigata	RLG00000033303
rosa_multiflora	Rmu_co8183894.1_g000001 Rmu_sc0000882.1_g000021 Rmu_sc0007782.1_g000010 Rmu_sc0018148.1_g000004 Rmu_sc0041046.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048950 Rroxscaffold_1G00052450
rosa_rugosa	Rorug05G0096800 Rorug05G0096800 Rorug05G0096800 Rorug05G0096800 Rorug05G0124800 Rorug05G0124900 Rorug05G0125000 Rorug05G0125000
rosa_samantha	Rh5AG190400 Rh5AG217500 Rh5BG188000 Rh5CG207800 Rh5DG189800
rosa_wichuraiana	Rw5G017340 Rw5G019890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	646, 1037
AccI	GTMKAC	2 cut(s)	357, 828
AccII	CGCG	1 cut(s)	666
AciI	CCGC	5 cut(s)	24, 666, 1083, 1194, 1219
AclWI	GGATC	1 cut(s)	1020
AcsI	RAATTY	3 cut(s)	209, 348, 561
AcuI	CTGAAG	1 cut(s)	1195
AcvI	CACGTG	1 cut(s)	620
AfaI	GTAC	3 cut(s)	492, 1023, 1109
AfiI	CCNNNNNNNGG	1 cut(s)	484
AflII	CTTAAG	1 cut(s)	19
AflIII	ACRYGT	2 cut(s)	617, 619
AgsI	TTSAA	4 cut(s)	335, 386, 782, 1116
AjiI	CACGTC	1 cut(s)	280
AjnI	CCWGG	2 cut(s)	988, 1045
AjuI	GAANNNNNNNTTGG	2 cut(s)	156, 188
AleI	CACNNNNGTG	1 cut(s)	620
AluBI	AGCT	6 cut(s)	236, 316, 699, 814, 916, 1139
AluI	AGCT	6 cut(s)	236, 316, 699, 814, 916, 1139
Alw21I	GWGCWC	2 cut(s)	318, 711
Alw26I	GTCTC	2 cut(s)	893, 1049
AlwI	GGATC	1 cut(s)	1020
AlwNI	CAGNNNCTG	2 cut(s)	775, 1142
Ama87I	CYCGRG	1 cut(s)	368
AoxI	GGCC	3 cut(s)	190, 920, 1027
ApeKI	GCWGC	3 cut(s)	215, 1139, 1142
ApoI	RAATTY	3 cut(s)	209, 348, 561
ArsI	GACNNNNNNTTYG	4 cut(s)	194, 226, 833, 865
AspS9I	GGNCC	2 cut(s)	190, 1028
AsuC2I	CCSGG	1 cut(s)	54
AsuHPI	GGTGA	2 cut(s)	265, 857
AsuII	TTCGAA	1 cut(s)	1169
AvaI	CYCGRG	1 cut(s)	368
BanI	GGYRCC	2 cut(s)	646, 1037
BanII	GRGCYC	1 cut(s)	318
BbrPI	CACGTG	1 cut(s)	620
Bbv12I	GWGCWC	2 cut(s)	318, 711
BbvCI	CCTCAGC	3 cut(s)	858, 1135, 1189
BbvI	GCAGC	3 cut(s)	202, 1126, 1129
BccI	CCATC	1 cut(s)	931
BciT130I	CCWGG	2 cut(s)	990, 1047
BcnI	CCSGG	1 cut(s)	54
BcoDI	GTCTC	2 cut(s)	893, 1049
BfaI	CTAG	2 cut(s)	308, 435
BfrI	CTTAAG	1 cut(s)	19
BglI	GCCNNNNNGGC	1 cut(s)	1035
BisI	GCNGC	4 cut(s)	216, 1140, 1143, 1194
BlsI	GCNGC	4 cut(s)	217, 1141, 1144, 1195
Bme1390I	CCNGG	3 cut(s)	54, 990, 1047
BmeT110I	CYCGRG	1 cut(s)	368
BmgBI	CACGTC	1 cut(s)	280
BmgT120I	GGNCC	2 cut(s)	190, 1028
BmiI	GGNNCC	2 cut(s)	648, 1039
BmrFI	CCNGG	3 cut(s)	54, 990, 1047
BmsI	GCATC	3 cut(s)	936, 1088, 1224
BpmI	CTGGAG	1 cut(s)	401
Bpu10I	CCTNAGC	5 cut(s)	858, 917, 1090, 1135, 1189
Bpu14I	TTCGAA	1 cut(s)	1169
BpuMI	CCSGG	1 cut(s)	54
BsaAI	YACGTR	1 cut(s)	620
BsaI	GGTCTC	1 cut(s)	893
BsaJI	CCNNGG	1 cut(s)	936
Bsc4I	CCNNNNNNNGG	1 cut(s)	484
Bse1I	ACTGG	2 cut(s)	418, 1105
Bse3DI	GCAATG	1 cut(s)	457
BseBI	CCWGG	2 cut(s)	990, 1047
BseDI	CCNNGG	1 cut(s)	936
BseGI	GGATG	3 cut(s)	923, 1079, 1215
BseLI	CCNNNNNNNGG	1 cut(s)	484
BseMI	GCAATG	1 cut(s)	457
BseMII	CTCAG	7 cut(s)	261, 849, 931, 1104, 1110, 1149, 1203
BseNI	ACTGG	2 cut(s)	418, 1105
BseRI	GAGGAG	3 cut(s)	527, 875, 881
BseXI	GCAGC	3 cut(s)	202, 1126, 1129
BsgI	GTGCAG	2 cut(s)	880, 1071
Bsh1236I	CGCG	1 cut(s)	666
BshFI	GGCC	3 cut(s)	192, 922, 1029
BshNI	GGYRCC	2 cut(s)	646, 1037
BsiHKAI	GWGCWC	2 cut(s)	318, 711
BsiHKCI	CYCGRG	1 cut(s)	368
BsiSI	CCGG	1 cut(s)	54
BslI	CCNNNNNNNGG	1 cut(s)	484
BsmAI	GTCTC	2 cut(s)	893, 1049
BsmBI	CGTCTC	1 cut(s)	1049
BsmI	GAATGC	1 cut(s)	84
BsnI	GGCC	3 cut(s)	192, 922, 1029
Bso31I	GGTCTC	1 cut(s)	893
BsoBI	CYCGRG	1 cut(s)	368
Bsp119I	TTCGAA	1 cut(s)	1169
Bsp1286I	GDGCHC	2 cut(s)	318, 711
Bsp143I	GATC	3 cut(s)	282, 882, 1012
BspACI	CCGC	5 cut(s)	24, 666, 1083, 1194, 1219
BspANI	GGCC	3 cut(s)	192, 922, 1029
BspCNI	CTCAG	7 cut(s)	262, 850, 930, 1103, 1109, 1148, 1202
BspFNI	CGCG	1 cut(s)	666
BspHI	TCATGA	1 cut(s)	481
BspLI	GGNNCC	2 cut(s)	648, 1039
BspPI	GGATC	1 cut(s)	1020
BspT104I	TTCGAA	1 cut(s)	1169
BspT107I	GGYRCC	2 cut(s)	646, 1037
BspTI	CTTAAG	1 cut(s)	19
BspTNI	GGTCTC	1 cut(s)	893
BsrDI	GCAATG	1 cut(s)	457
BsrI	ACTGG	2 cut(s)	418, 1105
BssECI	CCNNGG	1 cut(s)	936
BssMI	GATC	3 cut(s)	282, 882, 1012
Bst2UI	CCWGG	2 cut(s)	990, 1047
Bst4CI	ACNGT	3 cut(s)	771, 845, 937
BstAFI	CTTAAG	1 cut(s)	19
BstBAI	YACGTR	1 cut(s)	620
BstBI	TTCGAA	1 cut(s)	1169
BstC8I	GCNNGC	3 cut(s)	471, 526, 1087
BstDEI	CTNAG	8 cut(s)	270, 858, 917, 1090, 1096, 1135, 1189, 1203
BstDSI	CCRYGG	1 cut(s)	936
BstENI	CCTNNNNNAGG	1 cut(s)	482
BstF5I	GGATG	3 cut(s)	923, 1079, 1215
BstFNI	CGCG	1 cut(s)	666
BstKTI	GATC	3 cut(s)	285, 885, 1015
BstMAI	GTCTC	2 cut(s)	893, 1049
BstMBI	GATC	3 cut(s)	282, 882, 1012
BstMWI	GCNNNNNNNGC	5 cut(s)	436, 820, 863, 1035, 1136
BstNI	CCWGG	2 cut(s)	990, 1047
BstNSI	RCATGY	1 cut(s)	465
BstSCI	CCNGG	3 cut(s)	52, 988, 1045
BstUI	CGCG	1 cut(s)	666
BstV1I	GCAGC	3 cut(s)	202, 1126, 1129
BstX2I	RGATCY	1 cut(s)	1012
BstYI	RGATCY	1 cut(s)	1012
BsuRI	GGCC	3 cut(s)	192, 922, 1029
BtgI	CCRYGG	1 cut(s)	936
BtrI	CACGTC	1 cut(s)	280
BtsCI	GGATG	3 cut(s)	923, 1079, 1215
BtsIMutI	CAGTG	1 cut(s)	733
Cac8I	GCNNGC	3 cut(s)	471, 526, 1087
CaiI	CAGNNNCTG	2 cut(s)	775, 1142
CciI	TCATGA	1 cut(s)	481
Cfr13I	GGNCC	2 cut(s)	190, 1028
CseI	GACGC	1 cut(s)	863
Csp6I	GTAC	3 cut(s)	491, 1022, 1108
CviAII	CATG	9 cut(s)	299, 462, 482, 601, 608, 789, 928, 1009, 1034
CviQI	GTAC	3 cut(s)	491, 1022, 1108
DdeI	CTNAG	8 cut(s)	270, 858, 917, 1090, 1096, 1135, 1189, 1203
DpnI	GATC	3 cut(s)	284, 884, 1014
DpnII	GATC	3 cut(s)	282, 882, 1012
Ecl136II	GAGCTC	1 cut(s)	316
Eco24I	GRGCYC	1 cut(s)	318
Eco31I	GGTCTC	1 cut(s)	893
Eco32I	GATATC	1 cut(s)	517
Eco53kI	GAGCTC	1 cut(s)	316
Eco57I	CTGAAG	1 cut(s)	1195
Eco72I	CACGTG	1 cut(s)	620
Eco88I	CYCGRG	1 cut(s)	368
EcoICRI	GAGCTC	1 cut(s)	316
EcoNI	CCTNNNNNAGG	1 cut(s)	482
EcoO109I	RGGNCCY	1 cut(s)	1028
EcoRI	GAATTC	1 cut(s)	348
EcoRII	CCWGG	2 cut(s)	988, 1045
EcoRV	GATATC	1 cut(s)	517
EcoT22I	ATGCAT	1 cut(s)	1081
EcoT38I	GRGCYC	1 cut(s)	318
Esp3I	CGTCTC	1 cut(s)	1049
FaeI	CATG	9 cut(s)	302, 465, 485, 604, 611, 792, 931, 1012, 1037
FalI	AAGNNNNNCTT	2 cut(s)	977, 1009
FatI	CATG	9 cut(s)	298, 461, 481, 600, 607, 788, 927, 1008, 1033
FauNDI	CATATG	1 cut(s)	1226
FblI	GTMKAC	2 cut(s)	357, 828
Fnu4HI	GCNGC	4 cut(s)	216, 1140, 1143, 1194
FokI	GGATG	3 cut(s)	910, 1066, 1202
FriOI	GRGCYC	1 cut(s)	318
Fsp4HI	GCNGC	4 cut(s)	216, 1140, 1143, 1194
FspBI	CTAG	2 cut(s)	308, 435
GluI	GCNGC	4 cut(s)	216, 1140, 1143, 1194
GsuI	CTGGAG	1 cut(s)	401
HaeIII	GGCC	3 cut(s)	192, 922, 1029
HapII	CCGG	1 cut(s)	54
HgaI	GACGC	1 cut(s)	863
Hin1II	CATG	9 cut(s)	302, 465, 485, 604, 611, 792, 931, 1012, 1037
HincII	GTYRAC	1 cut(s)	933
HindII	GTYRAC	1 cut(s)	933
HinfI	GANTC	3 cut(s)	123, 239, 905
HpaII	CCGG	1 cut(s)	54
HphI	GGTGA	2 cut(s)	265, 857
Hpy166II	GTNNAC	6 cut(s)	37, 358, 497, 829, 933, 970
Hpy188I	TCNGA	7 cut(s)	61, 568, 912, 1003, 1019, 1099, 1183
Hpy188III	TCNNGA	5 cut(s)	45, 243, 370, 482, 943
Hpy8I	GTNNAC	6 cut(s)	37, 358, 497, 829, 933, 970
Hpy99I	CGWCG	2 cut(s)	845, 1063
HpyAV	CCTTC	9 cut(s)	25, 158, 250, 329, 484, 488, 955, 1129, 1171
HpyCH4III	ACNGT	3 cut(s)	771, 845, 937
HpyCH4IV	ACGT	3 cut(s)	279, 493, 619
HpyCH4V	TGCA	7 cut(s)	450, 461, 581, 659, 897, 1052, 1079
HpyF10VI	GCNNNNNNNGC	5 cut(s)	436, 820, 863, 1035, 1136
HpyF3I	CTNAG	8 cut(s)	270, 858, 917, 1090, 1096, 1135, 1189, 1203
HpySE526I	ACGT	3 cut(s)	279, 493, 619
Hsp92II	CATG	9 cut(s)	302, 465, 485, 604, 611, 792, 931, 1012, 1037
Kzo9I	GATC	3 cut(s)	282, 882, 1012
LmnI	GCTCC	2 cut(s)	714, 811
Lsp1109I	GCAGC	3 cut(s)	202, 1126, 1129
LweI	GCATC	3 cut(s)	936, 1088, 1224
MaeI	CTAG	2 cut(s)	308, 435
MaeII	ACGT	3 cut(s)	279, 493, 619
MalI	GATC	3 cut(s)	284, 884, 1014
MboI	GATC	3 cut(s)	282, 882, 1012
MboII	GAAGA	2 cut(s)	83, 1059
MfeI	CAATTG	2 cut(s)	671, 759
MflI	RGATCY	1 cut(s)	1012
MhlI	GDGCHC	2 cut(s)	318, 711
MluCI	AATT	7 cut(s)	30, 209, 348, 561, 671, 759, 1111
MlyI	GAGTC	1 cut(s)	899
Mph1103I	ATGCAT	1 cut(s)	1081
MseI	TTAA	4 cut(s)	20, 96, 198, 995
MslI	CAYNNNNRTG	1 cut(s)	620
MspA1I	CMGCKG	3 cut(s)	236, 1085, 1139
MspCI	CTTAAG	1 cut(s)	19
MspI	CCGG	1 cut(s)	54
MspR9I	CCNGG	3 cut(s)	54, 990, 1047
MunI	CAATTG	2 cut(s)	671, 759
Mva1269I	GAATGC	1 cut(s)	84
MvaI	CCWGG	2 cut(s)	990, 1047
MvnI	CGCG	1 cut(s)	666
MwoI	GCNNNNNNNGC	5 cut(s)	436, 820, 863, 1035, 1136
NciI	CCSGG	1 cut(s)	54
NdeI	CATATG	1 cut(s)	1226
NdeII	GATC	3 cut(s)	282, 882, 1012
NlaIII	CATG	9 cut(s)	302, 465, 485, 604, 611, 792, 931, 1012, 1037
NlaIV	GGNNCC	2 cut(s)	648, 1039
NsiI	ATGCAT	1 cut(s)	1081
NspI	RCATGY	1 cut(s)	465
NspV	TTCGAA	1 cut(s)	1169
OliI	CACNNNNGTG	1 cut(s)	620
PaeR7I	CTCGAG	1 cut(s)	368
PagI	TCATGA	1 cut(s)	481
PcsI	WCGNNNNNNNCGW	1 cut(s)	132
PctI	GAATGC	1 cut(s)	84
PfeI	GAWTC	2 cut(s)	123, 239
PkrI	GCNGC	4 cut(s)	217, 1141, 1144, 1195
PleI	GAGTC	1 cut(s)	899
PmaCI	CACGTG	1 cut(s)	620
PmlI	CACGTG	1 cut(s)	620
PpsI	GAGTC	1 cut(s)	899
Ppu21I	YACGTR	1 cut(s)	620
Psp124BI	GAGCTC	1 cut(s)	318
Psp6I	CCWGG	2 cut(s)	988, 1045
PspCI	CACGTG	1 cut(s)	620
PspGI	CCWGG	2 cut(s)	988, 1045
PspN4I	GGNNCC	2 cut(s)	648, 1039
PspPI	GGNCC	2 cut(s)	190, 1028
PstNI	CAGNNNCTG	2 cut(s)	775, 1142
PsuI	RGATCY	1 cut(s)	1012
PvuII	CAGCTG	2 cut(s)	236, 1139
RsaI	GTAC	3 cut(s)	492, 1023, 1109
RsaNI	GTAC	3 cut(s)	491, 1022, 1108
RseI	CAYNNNNRTG	1 cut(s)	620
SacI	GAGCTC	1 cut(s)	318
SaqAI	TTAA	4 cut(s)	20, 96, 198, 995
SatI	GCNGC	4 cut(s)	216, 1140, 1143, 1194
Sau3AI	GATC	3 cut(s)	282, 882, 1012
Sau96I	GGNCC	2 cut(s)	190, 1028
SchI	GAGTC	1 cut(s)	899
ScrFI	CCNGG	3 cut(s)	54, 990, 1047
SduI	GDGCHC	2 cut(s)	318, 711
SfaNI	GCATC	3 cut(s)	936, 1088, 1224
Sfr274I	CTCGAG	1 cut(s)	368
SfuI	TTCGAA	1 cut(s)	1169
SlaI	CTCGAG	1 cut(s)	368
SmiMI	CAYNNNNRTG	1 cut(s)	620
SmlI	CTYRAG	2 cut(s)	19, 368
SmoI	CTYRAG	2 cut(s)	19, 368
Sse9I	AATT	7 cut(s)	30, 209, 348, 561, 671, 759, 1111
SsiI	CCGC	5 cut(s)	24, 666, 1083, 1194, 1219
SspI	AATATT	1 cut(s)	532
SspMI	CTAG	2 cut(s)	308, 435
SstI	GAGCTC	1 cut(s)	318
StyD4I	CCNGG	3 cut(s)	52, 988, 1045
TaaI	ACNGT	3 cut(s)	771, 845, 937
TaiI	ACGT	3 cut(s)	282, 496, 622
TaqI	TCGA	4 cut(s)	369, 540, 840, 1169
TaqII	GACCGA	1 cut(s)	875
TasI	AATT	7 cut(s)	30, 209, 348, 561, 671, 759, 1111
TatI	WGTACW	1 cut(s)	1107
TauI	GCSGC	1 cut(s)	1196
TfiI	GAWTC	2 cut(s)	123, 239
Tru1I	TTAA	4 cut(s)	20, 96, 198, 995
Tru9I	TTAA	4 cut(s)	20, 96, 198, 995
TscAI	CASTG	1 cut(s)	733
TseI	GCWGC	3 cut(s)	215, 1139, 1142
TspDTI	ATGAA	6 cut(s)	84, 315, 470, 497, 997, 1153
TspGWI	ACGGA	1 cut(s)	820
TspRI	CASTG	1 cut(s)	733
Vha464I	CTTAAG	1 cut(s)	19
XagI	CCTNNNNNAGG	1 cut(s)	482
XapI	RAATTY	3 cut(s)	209, 348, 561
XceI	RCATGY	1 cut(s)	465
XcmI	CCANNNNNNNNNTGG	1 cut(s)	934
XhoI	CTCGAG	1 cut(s)	368
XmiI	GTMKAC	2 cut(s)	357, 828
XspI	CTAG	2 cut(s)	308, 435
Zsp2I	ATGCAT	1 cut(s)	1081

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.