Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002369

Physical Location & Seq

Genomic Coordinates Reverse (-)

40928174 .. 40932364

Gene Structure 4191 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1274700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

ATGAAGAAGAAGCTCAACTGGTGGGACCTCATCTGGTTCGGAATTGGCGTTGTCATCGGCGCCGGAATATTCGCGCTCACAGGACTGGAGGCGAGAAGGCACGCAGGCGCCGCCATCGTGCTGTCGTACGTCGTCTCGGGCGTCTCCGCCATGCTCTCTGTTTTCTGTTACACCGAGTTCGCCGTCGAGATTCCTGTTGCAGGTGGCTCATTTGCCTACCTTAGGGTGGAGCTTGGGGACTTCGTGGCCTTCATTGCAGCAGGCAACATCCTCCTCGAGTACGTCATTAGTGGCGCAGCCGTTGCTCGTTCCTGGACATCCTACTTCACCACCCTCTGCAACCGCAGCGACACCAATGATTTCCGCATCATAGCCCACGGTTTCCCAGAGGACTACAAGTACCTCGATCCCATTGCTGTCGCTGTCCTTATTTGCATCTGCTGCCTTGCAGTCCTCAGCATAAAGGGCTCTTCTCGTCTCAACTACATTGCCTCGATTTTCCATATAATTGTCATTGTCTTCATCATCATCGCCGGCCTCACCAAAGCTGATACCAAAAACTACATCGATTTCGCCCCTTTTGGCCCGCGCGGCGTGTTCCAAGCGTCGGCTGTTGTGTTCTTCGCGTACGTTGGATTTGATGCCGTTTCAACCATGGCGGAGGAGACCAAGAATCCCGCAAAGGACATCCCAATTGGTCTTGTCGGCTCAATGGTGATTGTTACAGTGCTCTATTGCTTGCTTGCACTCACATTAGGCCTTATGCAGCCATATAACCAAATCGACGAACAAGCTGCATTTCCGGTTGCGTTTCAAGCTGTTGGGATGGGATGGGCTAAGTACCTTGTGGCAGCGGGCGCATTGGAAGGCATGACAAGCGCGCTGCTTGGCGGGGCAGTTGGACAGGCGCGGTACCTCACTCACATTGCCCGAACCCACATGGTGCCACCATGGTTTGCACAAGTTGATTCCAAAACCGGCACTCCTGTCAATGCCACGGTCACCTTACTCGCAGCCACGGCAGTCATTGCCTTCTTCACGAGCCTCGACGTTCTGGCCAACCTTGTCTCCATCTCCACTTTGTTCATCTTCAGCCTTGTTGCCATCGCTCTCCTTGTTCGCCGCTACTATGTCAGCGGCGTCACAACTCAGGCCAACAGGAACAAGTTCATTGCCTGCCTTTTCATCATAATTAGCTCTTCAATTGCCACCTCTGTCTATTGGAGCAGAACAGATGATTGGATTGCCTACGTGGTAACCGCGCCGCTTTGGTTCTTGGGTACTTTAGGGATTTGGTTACTTGTCCCCCAAGCAAGGAGTCCGAAGCTCTGGGGAGTGCCATTGGTGCCATGGTTGCCATCTCTTTCAATTGCCATCAACATTTTCCTGCTCGGGTCAATCGATCACGCCTCATTCATAAGGTTTGGAGTGTGGACTCTGATCATCTTGGTTTACTACTTCATCTTCGGATTGCACGCGTCTTATGACACGGTAAAGGACTCAGAAGCGAAGAGGCTGGAGGGCGGCAGAAACAATGAGTTGAGGAAGGTTGAAGCTGCAAGGGTTGCCACGAGGGACTCCGGCTTAGACGGCGGAAGTGGTAGCAATGCCTCTGAAACTCCTGCTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AA_permease_2 AA_permease_C

Protein Analysis

544

Amino Acids

58.75

Weight (kDa)

8.06

Isoelectric Point (pI)

29.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease_2	PF13520	5 - 400	2.2e-50	Amino acid permease
AA_permease	PF00324	10 - 407	2.8e-35	Amino acid permease
AA_permease_C	PF13906	444 - 494	6.7e-19	C-terminus of AA_permease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002369)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21120 AT4G21120
fragaria_vesca	FvH4_3g09930 FvH4_3g09940
malus_domestica	MD05G1274600.v1.1 MD05G1274700.v1.1 MD05G1274900.v1.1 MD10G1254300.v1.1
prunus_persica	Prupe.4G089000_v2.0.a1 Prupe.4G089100_v2.0.a1
pyrus_communis	pycom05g25610 pycom05g25630 pycom10g21000
rosa_chinensis	RchiOBHm_Chr5g0015621 RchiOBHm_Chr5g0015661 RchiOBHm_Chr5g0015691 RchiOBHm_Chr5g0015791
rosa_laevigata	RLG00000032214 RLG00000032217 RLG00000032219
rosa_multiflora	Rmu_sc0000843.1_g000004 Rmu_sc0000843.1_g000008 Rmu_sc0003230.1_g000003 Rmu_sc0003230.1_g000005
rosa_roxburghii	Rroxscaffold_1G00060830
rosa_rugosa	Rorug01G0078300 Rorug05G0029000 Rorug05G0029100
rosa_samantha	Rh5AG122300 Rh5AG122400 Rh5AG122600 Rh5BG120900 Rh5BG121200 Rh5BG121700 Rh5CG131300 Rh5CG131900 Rh5CG132800 Rh5DG119900 Rh5DG120600
rosa_wichuraiana	Rw5G010550 Rw5G010560 Rw5G010570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	191
Acc36I	ACCTGC	1 cut(s)	191
Acc65I	GGTACC	1 cut(s)	912
AccB1I	GGYRCC	5 cut(s)	59, 107, 912, 943, 1345
AccII	CGCG	8 cut(s)	74, 589, 591, 626, 881, 910, 1262, 1478
AclWI	GGATC	1 cut(s)	401
AcoI	YGGCCR	1 cut(s)	1056
AcuI	CTGAAG	1 cut(s)	1075
AcyI	GRCGYC	4 cut(s)	60, 108, 141, 1140
AdeI	CACNNNGTG	1 cut(s)	943
AfaI	GTAC	7 cut(s)	128, 281, 401, 629, 842, 914, 1282
AfiI	CCNNNNNNNGG	5 cut(s)	200, 222, 226, 607, 1314
AflIII	ACRYGT	1 cut(s)	1476
AgsI	TTSAA	5 cut(s)	651, 815, 1203, 1368, 1553
AjnI	CCWGG	1 cut(s)	311
AjuI	GAANNNNNNNTTGG	2 cut(s)	1222, 1254
AluBI	AGCT	8 cut(s)	13, 232, 548, 794, 818, 1197, 1327, 1556
AluI	AGCT	8 cut(s)	13, 232, 548, 794, 818, 1197, 1327, 1556
Alw21I	GWGCWC	1 cut(s)	732
Alw26I	GTCTC	5 cut(s)	139, 148, 482, 659, 1072
AlwI	GGATC	1 cut(s)	401
Ama87I	CYCGRG	3 cut(s)	136, 275, 1391
AoxI	GGCC	6 cut(s)	246, 535, 583, 757, 1056, 1152
Asp700I	GAANNNNTTC	1 cut(s)	1166
Asp718I	GGTACC	1 cut(s)	912
AspS9I	GGNCC	2 cut(s)	25, 584
AsuHPI	GGTGA	4 cut(s)	319, 532, 727, 994
AvaI	CYCGRG	3 cut(s)	136, 275, 1391
AvaII	GGWCC	1 cut(s)	25
AxyI	CCTNAGG	1 cut(s)	221
BalI	TGGCCA	1 cut(s)	1058
BanI	GGYRCC	5 cut(s)	59, 107, 912, 943, 1345
BanII	GRGCYC	1 cut(s)	470
BauI	CACGAG	2 cut(s)	1039, 1570
BbsI	GAAGAC	1 cut(s)	511
Bbv12I	GWGCWC	1 cut(s)	732
BbvCI	CCTCAGC	1 cut(s)	455
BccI	CCATC	7 cut(s)	122, 820, 825, 1079, 1112, 1366, 1382
BceAI	ACGGC	5 cut(s)	167, 284, 629, 1035, 1606
BcgI	CGANNNNNNTGC	4 cut(s)	395, 429, 776, 810
BciT130I	CCWGG	1 cut(s)	313
BclI	TGATCA	1 cut(s)	1440
BcoDI	GTCTC	5 cut(s)	139, 148, 482, 659, 1072
BfaI	CTAG	1 cut(s)	1626
BfoI	RGCGCY	2 cut(s)	63, 111
BfuAI	ACCTGC	1 cut(s)	191
BglI	GCCNNNNNGGC	1 cut(s)	591
Bme1390I	CCNGG	1 cut(s)	313
Bme18I	GGWCC	1 cut(s)	25
BmeT110I	CYCGRG	3 cut(s)	136, 275, 1391
BmgT120I	GGNCC	2 cut(s)	25, 584
BmiI	GGNNCC	6 cut(s)	26, 61, 109, 914, 945, 1347
BmrFI	CCNGG	1 cut(s)	313
BmsI	GCATC	3 cut(s)	375, 444, 631
BpiI	GAAGAC	1 cut(s)	511
BpmI	CTGGAG	2 cut(s)	107, 1538
Bpu10I	CCTNAGC	1 cut(s)	455
Bsa29I	ATCGAT	2 cut(s)	567, 1401
BsaAI	YACGTR	1 cut(s)	1252
BsaHI	GRCGYC	4 cut(s)	60, 108, 141, 1140
BsaI	GGTCTC	1 cut(s)	659
BsaJI	CCNNGG	6 cut(s)	376, 654, 950, 996, 1017, 1349
BsaWI	WCCGGW	1 cut(s)	802
BsaXI	ACNNNNNCTCC	4 cut(s)	967, 997, 1052, 1082
Bsc4I	CCNNNNNNNGG	5 cut(s)	200, 222, 226, 607, 1314
Bse118I	RCCGGY	2 cut(s)	533, 977
Bse1I	ACTGG	2 cut(s)	23, 90
Bse21I	CCTNAGG	1 cut(s)	221
Bse3DI	GCAATG	7 cut(s)	252, 411, 486, 924, 1026, 1170, 1612
BseBI	CCWGG	1 cut(s)	313
BseCI	ATCGAT	2 cut(s)	567, 1401
BseDI	CCNNGG	6 cut(s)	376, 654, 950, 996, 1017, 1349
BseGI	GGATG	5 cut(s)	267, 317, 687, 831, 836
BseLI	CCNNNNNNNGG	5 cut(s)	200, 222, 226, 607, 1314
BseMI	GCAATG	7 cut(s)	252, 411, 486, 924, 1026, 1170, 1612
BseMII	CTCAG	3 cut(s)	469, 1163, 1515
BseNI	ACTGG	2 cut(s)	23, 90
BsePI	GCGCGC	1 cut(s)	879
BseRI	GAGGAG	2 cut(s)	263, 677
Bsh1236I	CGCG	8 cut(s)	74, 589, 591, 626, 881, 910, 1262, 1478
BshFI	GGCC	6 cut(s)	248, 537, 585, 759, 1058, 1154
BshNI	GGYRCC	5 cut(s)	59, 107, 912, 943, 1345
BshVI	ATCGAT	2 cut(s)	567, 1401
BsiHKAI	GWGCWC	1 cut(s)	732
BsiHKCI	CYCGRG	3 cut(s)	136, 275, 1391
BsiSI	CCGG	5 cut(s)	63, 534, 803, 978, 1581
BsiWI	CGTACG	2 cut(s)	126, 627
BslFI	GGGAC	4 cut(s)	38, 251, 1289, 1589
BslI	CCNNNNNNNGG	5 cut(s)	200, 222, 226, 607, 1314
BsmAI	GTCTC	5 cut(s)	139, 148, 482, 659, 1072
BsmBI	CGTCTC	3 cut(s)	139, 148, 482
BsmFI	GGGAC	4 cut(s)	38, 251, 1289, 1589
BsnI	GGCC	6 cut(s)	248, 537, 585, 759, 1058, 1154
Bso31I	GGTCTC	1 cut(s)	659
BsoBI	CYCGRG	3 cut(s)	136, 275, 1391
Bsp1286I	GDGCHC	2 cut(s)	470, 732
Bsp143I	GATC	3 cut(s)	406, 1402, 1440
Bsp19I	CCATGG	3 cut(s)	654, 950, 1349
BspANI	GGCC	6 cut(s)	248, 537, 585, 759, 1058, 1154
BspCNI	CTCAG	3 cut(s)	468, 1162, 1514
BspDI	ATCGAT	2 cut(s)	567, 1401
BspFNI	CGCG	8 cut(s)	74, 589, 591, 626, 881, 910, 1262, 1478
BspLI	GGNNCC	6 cut(s)	26, 61, 109, 914, 945, 1347
BspMI	ACCTGC	1 cut(s)	191
BspPI	GGATC	1 cut(s)	401
BspQI	GCTCTTC	2 cut(s)	475, 1204
BspT107I	GGYRCC	5 cut(s)	59, 107, 912, 943, 1345
BspTNI	GGTCTC	1 cut(s)	659
BsrDI	GCAATG	7 cut(s)	252, 411, 486, 924, 1026, 1170, 1612
BsrFI	RCCGGY	2 cut(s)	533, 977
BsrI	ACTGG	2 cut(s)	23, 90
BssAI	RCCGGY	2 cut(s)	533, 977
BssECI	CCNNGG	6 cut(s)	376, 654, 950, 996, 1017, 1349
BssHII	GCGCGC	1 cut(s)	879
BssMI	GATC	3 cut(s)	406, 1402, 1440
BssNI	GRCGYC	4 cut(s)	60, 108, 141, 1140
BssSI	CACGAG	2 cut(s)	1039, 1570
BssT1I	CCWWGG	3 cut(s)	654, 950, 1349
Bst2BI	CACGAG	2 cut(s)	1039, 1570
Bst2UI	CCWGG	1 cut(s)	313
Bst4CI	ACNGT	4 cut(s)	380, 727, 1000, 1492
Bst6I	CTCTTC	3 cut(s)	475, 1204, 1505
BstACI	GRCGYC	4 cut(s)	60, 108, 141, 1140
BstAPI	GCANNNNNTGC	4 cut(s)	302, 441, 1028, 1565
BstBAI	YACGTR	1 cut(s)	1252
BstDEI	CTNAG	6 cut(s)	221, 455, 837, 1149, 1501, 1585
BstDSI	CCRYGG	6 cut(s)	376, 654, 950, 996, 1017, 1349
BstEII	GGTNACC	2 cut(s)	1000, 1255
BstENI	CCTNNNNNAGG	2 cut(s)	198, 220
BstF5I	GGATG	5 cut(s)	267, 317, 687, 831, 836
BstFNI	CGCG	8 cut(s)	74, 589, 591, 626, 881, 910, 1262, 1478
BstH2I	RGCGCY	2 cut(s)	63, 111
BstKTI	GATC	3 cut(s)	409, 1405, 1443
BstMAI	GTCTC	5 cut(s)	139, 148, 482, 659, 1072
BstMBI	GATC	3 cut(s)	406, 1402, 1440
BstNI	CCWGG	1 cut(s)	313
BstPI	GGTNACC	2 cut(s)	1000, 1255
BstSCI	CCNGG	1 cut(s)	311
BstUI	CGCG	8 cut(s)	74, 589, 591, 626, 881, 910, 1262, 1478
BstV2I	GAAGAC	1 cut(s)	511
Bsu15I	ATCGAT	2 cut(s)	567, 1401
Bsu36I	CCTNAGG	1 cut(s)	221
BsuRI	GGCC	6 cut(s)	248, 537, 585, 759, 1058, 1154
BsuTUI	ATCGAT	2 cut(s)	567, 1401
BtgI	CCRYGG	6 cut(s)	376, 654, 950, 996, 1017, 1349
BtgZI	GCGATG	2 cut(s)	514, 1090
BtsCI	GGATG	5 cut(s)	267, 317, 687, 831, 836
BtsIMutI	CAGTG	1 cut(s)	732
BveI	ACCTGC	1 cut(s)	191
Cfr10I	RCCGGY	2 cut(s)	533, 977
Cfr13I	GGNCC	2 cut(s)	25, 584
ClaI	ATCGAT	2 cut(s)	567, 1401
CseI	GACGC	4 cut(s)	130, 594, 1129, 1467
Csp6I	GTAC	7 cut(s)	127, 280, 400, 628, 841, 913, 1281
CviAII	CATG	6 cut(s)	151, 655, 871, 940, 951, 1350
CviQI	GTAC	7 cut(s)	127, 280, 400, 628, 841, 913, 1281
DdeI	CTNAG	6 cut(s)	221, 455, 837, 1149, 1501, 1585
DinI	GGCGCC	2 cut(s)	61, 109
DpnI	GATC	3 cut(s)	408, 1404, 1442
DpnII	GATC	3 cut(s)	406, 1402, 1440
DraIII	CACNNNGTG	1 cut(s)	943
EaeI	YGGCCR	1 cut(s)	1056
Eam1104I	CTCTTC	3 cut(s)	475, 1204, 1505
EarI	CTCTTC	3 cut(s)	475, 1204, 1505
EciI	GGCGGA	3 cut(s)	136, 674, 1608
Eco130I	CCWWGG	3 cut(s)	654, 950, 1349
Eco147I	AGGCCT	1 cut(s)	759
Eco24I	GRGCYC	1 cut(s)	470
Eco31I	GGTCTC	1 cut(s)	659
Eco47I	GGWCC	1 cut(s)	25
Eco57I	CTGAAG	1 cut(s)	1075
Eco81I	CCTNAGG	1 cut(s)	221
Eco88I	CYCGRG	3 cut(s)	136, 275, 1391
Eco91I	GGTNACC	2 cut(s)	1000, 1255
EcoNI	CCTNNNNNAGG	2 cut(s)	198, 220
EcoO109I	RGGNCCY	1 cut(s)	25
EcoO65I	GGTNACC	2 cut(s)	1000, 1255
EcoRII	CCWGG	1 cut(s)	311
EcoT14I	CCWWGG	3 cut(s)	654, 950, 1349
EcoT38I	GRGCYC	1 cut(s)	470
EgeI	GGCGCC	2 cut(s)	61, 109
EheI	GGCGCC	2 cut(s)	61, 109
ErhI	CCWWGG	3 cut(s)	654, 950, 1349
Esp3I	CGTCTC	3 cut(s)	139, 148, 482
FaeI	CATG	6 cut(s)	154, 658, 874, 943, 954, 1353
FaqI	GGGAC	4 cut(s)	38, 251, 1289, 1589
FatI	CATG	6 cut(s)	150, 654, 870, 939, 950, 1349
FauI	CCCGC	4 cut(s)	594, 685, 847, 884
FbaI	TGATCA	1 cut(s)	1440
FokI	GGATG	5 cut(s)	254, 304, 674, 838, 843
FriOI	GRGCYC	1 cut(s)	470
FspBI	CTAG	1 cut(s)	1626
GsuI	CTGGAG	2 cut(s)	107, 1538
HaeII	RGCGCY	2 cut(s)	63, 111
HaeIII	GGCC	6 cut(s)	248, 537, 585, 759, 1058, 1154
HapII	CCGG	5 cut(s)	63, 534, 803, 978, 1581
HgaI	GACGC	4 cut(s)	130, 594, 1129, 1467
Hin1I	GRCGYC	4 cut(s)	60, 108, 141, 1140
Hin1II	CATG	6 cut(s)	154, 658, 874, 943, 954, 1353
HinfI	GANTC	7 cut(s)	190, 673, 968, 1318, 1435, 1499, 1577
HpaII	CCGG	5 cut(s)	63, 534, 803, 978, 1581
HphI	GGTGA	4 cut(s)	319, 532, 727, 994
Hpy166II	GTNNAC	2 cut(s)	1434, 1453
Hpy188I	TCNGA	6 cut(s)	41, 1323, 1440, 1469, 1504, 1615
Hpy188III	TCNNGA	2 cut(s)	187, 1039
Hpy8I	GTNNAC	2 cut(s)	1434, 1453
Hpy99I	CGWCG	5 cut(s)	134, 188, 610, 788, 1052
HpyAV	CCTTC	5 cut(s)	90, 259, 860, 1042, 1540
HpyCH4III	ACNGT	4 cut(s)	380, 727, 1000, 1492
HpyCH4IV	ACGT	5 cut(s)	129, 282, 630, 1050, 1251
HpyF3I	CTNAG	6 cut(s)	221, 455, 837, 1149, 1501, 1585
HpySE526I	ACGT	5 cut(s)	129, 282, 630, 1050, 1251
Hsp92I	GRCGYC	4 cut(s)	60, 108, 141, 1140
Hsp92II	CATG	6 cut(s)	154, 658, 874, 943, 954, 1353
KasI	GGCGCC	2 cut(s)	59, 107
KpnI	GGTACC	1 cut(s)	916
KroI	GCCGGC	1 cut(s)	533
KroNI	GCCGGC	1 cut(s)	535
Ksp22I	TGATCA	1 cut(s)	1440
Kzo9I	GATC	3 cut(s)	406, 1402, 1440
LguI	GCTCTTC	2 cut(s)	475, 1204
LmnI	GCTCC	2 cut(s)	229, 1224
LweI	GCATC	3 cut(s)	375, 444, 631
MaeI	CTAG	1 cut(s)	1626
MaeII	ACGT	5 cut(s)	129, 282, 630, 1050, 1251
MaeIII	GTNAC	6 cut(s)	167, 721, 1000, 1141, 1255, 1296
MalI	GATC	3 cut(s)	408, 1404, 1442
MboI	GATC	3 cut(s)	406, 1402, 1440
MfeI	CAATTG	3 cut(s)	693, 1203, 1368
MhlI	GDGCHC	2 cut(s)	470, 732
MlsI	TGGCCA	1 cut(s)	1058
MluCI	AATT	6 cut(s)	42, 507, 693, 1191, 1203, 1368
MluI	ACGCGT	1 cut(s)	1476
MluNI	TGGCCA	1 cut(s)	1058
Mly113I	GGCGCC	2 cut(s)	60, 108
MlyI	GAGTC	4 cut(s)	1327, 1429, 1493, 1571
MmeI	TCCRAC	2 cut(s)	613, 880
Mox20I	TGGCCA	1 cut(s)	1058
MroNI	GCCGGC	1 cut(s)	533
MroXI	GAANNNNTTC	1 cut(s)	1166
MscI	TGGCCA	1 cut(s)	1058
MseI	TTAA	1 cut(s)	1630
Msp20I	TGGCCA	1 cut(s)	1058
MspA1I	CMGCKG	2 cut(s)	854, 1137
MspI	CCGG	5 cut(s)	63, 534, 803, 978, 1581
MspR9I	CCNGG	1 cut(s)	313
MunI	CAATTG	3 cut(s)	693, 1203, 1368
MvaI	CCWGG	1 cut(s)	313
MvnI	CGCG	8 cut(s)	74, 589, 591, 626, 881, 910, 1262, 1478
NaeI	GCCGGC	1 cut(s)	535
NarI	GGCGCC	2 cut(s)	60, 108
NcoI	CCATGG	3 cut(s)	654, 950, 1349
NdeII	GATC	3 cut(s)	406, 1402, 1440
NgoMIV	GCCGGC	1 cut(s)	533
NlaIII	CATG	6 cut(s)	154, 658, 874, 943, 954, 1353
NlaIV	GGNNCC	6 cut(s)	26, 61, 109, 914, 945, 1347
NmuCI	GTSAC	2 cut(s)	1000, 1141
PaeR7I	CTCGAG	1 cut(s)	275
PaqCI	CACCTGC	1 cut(s)	191
PauI	GCGCGC	1 cut(s)	879
PceI	AGGCCT	1 cut(s)	759
PciSI	GCTCTTC	2 cut(s)	475, 1204
PcsI	WCGNNNNNNNCGW	3 cut(s)	45, 138, 1398
PdiI	GCCGGC	1 cut(s)	535
PdmI	GAANNNNTTC	1 cut(s)	1166
PfeI	GAWTC	3 cut(s)	190, 673, 968
Pfl23II	CGTACG	2 cut(s)	126, 627
PfoI	TCCNGGA	1 cut(s)	311
PleI	GAGTC	4 cut(s)	1326, 1429, 1493, 1571
PluTI	GGCGCC	2 cut(s)	63, 111
PpsI	GAGTC	4 cut(s)	1326, 1429, 1493, 1571
Ppu21I	YACGTR	1 cut(s)	1252
PpuMI	RGGWCCY	1 cut(s)	25
Psp5II	RGGWCCY	1 cut(s)	25
Psp6I	CCWGG	1 cut(s)	311
PspEI	GGTNACC	2 cut(s)	1000, 1255
PspGI	CCWGG	1 cut(s)	311
PspLI	CGTACG	2 cut(s)	126, 627
PspN4I	GGNNCC	6 cut(s)	26, 61, 109, 914, 945, 1347
PspPI	GGNCC	2 cut(s)	25, 584
PspPPI	RGGWCCY	1 cut(s)	25
PspXI	VCTCGAGB	1 cut(s)	275
PteI	GCGCGC	1 cut(s)	879
RsaI	GTAC	7 cut(s)	128, 281, 401, 629, 842, 914, 1282
RsaNI	GTAC	7 cut(s)	127, 280, 400, 628, 841, 913, 1281
SapI	GCTCTTC	2 cut(s)	475, 1204
SaqAI	TTAA	1 cut(s)	1630
Sau3AI	GATC	3 cut(s)	406, 1402, 1440
Sau96I	GGNCC	2 cut(s)	25, 584
SchI	GAGTC	4 cut(s)	1327, 1429, 1493, 1571
ScrFI	CCNGG	1 cut(s)	313
SduI	GDGCHC	2 cut(s)	470, 732
SfaNI	GCATC	3 cut(s)	375, 444, 631
SfoI	GGCGCC	2 cut(s)	61, 109
Sfr274I	CTCGAG	1 cut(s)	275
SinI	GGWCC	1 cut(s)	25
SlaI	CTCGAG	1 cut(s)	275
SmlI	CTYRAG	1 cut(s)	275
SmoI	CTYRAG	1 cut(s)	275
Sse9I	AATT	6 cut(s)	42, 507, 693, 1191, 1203, 1368
SseBI	AGGCCT	1 cut(s)	759
SspDI	GGCGCC	2 cut(s)	59, 107
SspI	AATATT	1 cut(s)	69
SspMI	CTAG	1 cut(s)	1626
StuI	AGGCCT	1 cut(s)	759
StyD4I	CCNGG	1 cut(s)	311
StyI	CCWWGG	3 cut(s)	654, 950, 1349
TaaI	ACNGT	4 cut(s)	380, 727, 1000, 1492
TaiI	ACGT	5 cut(s)	132, 285, 633, 1053, 1254
TaqI	TCGA	8 cut(s)	186, 276, 405, 494, 567, 783, 1047, 1401
TasI	AATT	6 cut(s)	42, 507, 693, 1191, 1203, 1368
TauI	GCSGC	6 cut(s)	113, 594, 1125, 1140, 1267, 1527
TfiI	GAWTC	3 cut(s)	190, 673, 968
Tru1I	TTAA	1 cut(s)	1630
Tru9I	TTAA	1 cut(s)	1630
TscAI	CASTG	1 cut(s)	732
TseFI	GTSAC	2 cut(s)	1000, 1141
Tsp45I	GTSAC	2 cut(s)	1000, 1141
TspDTI	ATGAA	8 cut(s)	17, 241, 511, 1075, 1159, 1174, 1405, 1450
TspRI	CASTG	1 cut(s)	732
VpaK11BI	GGWCC	1 cut(s)	25
XagI	CCTNNNNNAGG	2 cut(s)	198, 220
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1347
XhoI	CTCGAG	1 cut(s)	275
XmnI	GAANNNNTTC	1 cut(s)	1166
XspI	CTAG	1 cut(s)	1626

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.