RchiOBHm_Chr5g0015791

C-terminus of AA_permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002369
Physical Location & Seq
Reverse (-)
10942176 .. 10943237
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29621

Sequence Viewer

Length: 921 bp
ATGGGTGCAAGAAGCCAGAACGAAATGAAGAAAACCCTAAACTGGTGGGATCTCATTTGGTTTGGGATCGAAGCAGTCATGGAGGCAGGAATTTTCGTGCTCACCGGTGAGGCAGCCAGGAAACTTGTTGGCCCTGCAGTTGTAATCTCTTACTTGATATCAGGTGTTTCGGTATTACTCTCAGTTTTGTGCTACACTAAGTTTGTTGTGGAGCTTCCGGTGGCTGGAGGCTCATTTGCGTATCCTAAAGTGGAGCTTGGTGACTTCATGGCCTATATTGCTGCAGGGAACATTTTATTTGAGTATATAGTAGCCGGTGCTAGTGCATCAAGACATAGTCTGTGCTGTTTTTTTGCTTATGTAGGGTTTGATGGTGTGGCAACTTTGGCGGAGGGGGTAATTGGCTCAATAGTGATTACCATTGTAACTTATTCTGCTCTTGCAGCGACATTATGTATGATGCAACCTTACAGCCAAATTAACGCAGATGCATCATTCACTGTTGCATTTCAGGCCGCAGGTATGAACTGGGCAAAGTACATTGTTGCATTGGGAGCACTTAAAGGCATGTCCACGGTTCTACTAGCCAACCTCATCGGGCAGGCTCGATACTTCACTCACATCGGAAGAACTCACATGGCGCCACCAATCCTTGCAAGGATCAATGCCAAAACCGGTACCCCGGTAACTGCCACGATTATCATGATCGTTGCAAACTCAATTGTTGCTTTCTTCACAAGCCTTGATGTCTTGGCAAACCTTCTCTCTATATCAACTCTGTTTATATTTTCCCTTGTTGCATTAGCACTGATTGTTAGGCGGTACTACGTTACCGGTGAAACATCAGCAACAGACATAAACAAACTCATTGGTTTCTTGACACTAATCATAGGTGCATCAATTGCAACTGCTGGGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AA_permease_2 AA_permease_C

Protein Analysis

306

Amino Acids

32.57

Weight (kDa)

8.29

Isoelectric Point (pI)

20.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_permease PF00324 129 - 300 8.7e-06 Amino acid permease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 509
Acc65I GGTACC 1 cut(s) 677
AccB1I GGYRCC 2 cut(s) 640, 677
AciI CCGC 3 cut(s) 389, 516, 820
AclWI GGATC 3 cut(s) 57, 74, 668
AcsI RAATTY 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 641
AfaI GTAC 3 cut(s) 539, 679, 824
AfiI CCNNNNNNNGG 2 cut(s) 42, 224
AgeI ACCGGT 3 cut(s) 104, 674, 833
AjnI CCWGG 1 cut(s) 116
AluBI AGCT 2 cut(s) 214, 256
AluI AGCT 2 cut(s) 214, 256
Alw21I GWGCWC 2 cut(s) 102, 559
AlwI GGATC 3 cut(s) 57, 74, 668
AoxI GGCC 3 cut(s) 130, 270, 513
ApeKI GCWGC 3 cut(s) 113, 281, 443
ApoI RAATTY 1 cut(s) 90
AsiGI ACCGGT 3 cut(s) 104, 674, 833
Asp718I GGTACC 1 cut(s) 677
AspLEI GCGC 1 cut(s) 643
AspS9I GGNCC 1 cut(s) 131
AsuC2I CCSGG 1 cut(s) 683
AsuHPI GGTGA 4 cut(s) 94, 119, 272, 848
BaeI ACNNNNGTAYC 2 cut(s) 669, 702
BanI GGYRCC 2 cut(s) 640, 677
Bbv12I GWGCWC 2 cut(s) 102, 559
BbvI GCAGC 3 cut(s) 125, 268, 455
BccI CCATC 1 cut(s) 365
BciT130I CCWGG 1 cut(s) 118
BciVI GTATCC 1 cut(s) 252
BcnI CCSGG 1 cut(s) 683
BfaI CTAG 2 cut(s) 321, 584
BfmI CTRYAG 2 cut(s) 135, 282
BfoI RGCGCY 1 cut(s) 644
BfuAI ACCTGC 1 cut(s) 509
BfuI GTATCC 1 cut(s) 252
BisI GCNGC 4 cut(s) 114, 282, 444, 516
BlsI GCNGC 4 cut(s) 115, 283, 445, 517
Bme1390I CCNGG 2 cut(s) 118, 683
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 2 cut(s) 642, 679
BmrFI CCNGG 2 cut(s) 118, 683
BmrI ACTGGG 1 cut(s) 538
BmsI GCATC 5 cut(s) 335, 450, 478, 500, 905
BmuI ACTGGG 1 cut(s) 538
BpmI CTGGAG 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 683
BsaHI GRCGYC 1 cut(s) 641
BsaJI CCNNGG 2 cut(s) 573, 681
BsaWI WCCGGW 4 cut(s) 104, 217, 674, 833
Bsc4I CCNNNNNNNGG 2 cut(s) 42, 224
Bse118I RCCGGY 4 cut(s) 104, 314, 674, 833
Bse1I ACTGG 2 cut(s) 47, 533
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 2 cut(s) 573, 681
BseLI CCNNNNNNNGG 2 cut(s) 42, 224
BseMII CTCAG 1 cut(s) 195
BseNI ACTGG 2 cut(s) 47, 533
BseXI GCAGC 3 cut(s) 125, 268, 455
BseYI CCCAGC 1 cut(s) 911
BshFI GGCC 3 cut(s) 132, 272, 515
BshNI GGYRCC 2 cut(s) 640, 677
BshTI ACCGGT 3 cut(s) 104, 674, 833
BsiHKAI GWGCWC 2 cut(s) 102, 559
BsiSI CCGG 6 cut(s) 105, 218, 315, 675, 683, 834
BslI CCNNNNNNNGG 2 cut(s) 42, 224
BsnI GGCC 3 cut(s) 132, 272, 515
Bsp1286I GDGCHC 2 cut(s) 102, 559
Bsp143I GATC 4 cut(s) 49, 66, 660, 705
BspACI CCGC 3 cut(s) 389, 516, 820
BspANI GGCC 3 cut(s) 132, 272, 515
BspCNI CTCAG 1 cut(s) 194
BspHI TCATGA 1 cut(s) 702
BspLI GGNNCC 2 cut(s) 642, 679
BspMAI CTGCAG 2 cut(s) 139, 286
BspMI ACCTGC 1 cut(s) 509
BspPI GGATC 3 cut(s) 57, 74, 668
BspT107I GGYRCC 2 cut(s) 640, 677
BsrFI RCCGGY 4 cut(s) 104, 314, 674, 833
BsrI ACTGG 2 cut(s) 47, 533
BssAI RCCGGY 4 cut(s) 104, 314, 674, 833
BssECI CCNNGG 2 cut(s) 573, 681
BssMI GATC 4 cut(s) 49, 66, 660, 705
BssNI GRCGYC 1 cut(s) 641
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 2 cut(s) 502, 577
BstACI GRCGYC 1 cut(s) 641
BstAPI GCANNNNNTGC 1 cut(s) 902
BstC8I GCNNGC 1 cut(s) 603
BstDEI CTNAG 2 cut(s) 181, 198
BstDSI CCRYGG 1 cut(s) 573
BstH2I RGCGCY 1 cut(s) 644
BstHHI GCGC 1 cut(s) 643
BstKTI GATC 4 cut(s) 52, 69, 663, 708
BstMBI GATC 4 cut(s) 49, 66, 660, 705
BstMWI GCNNNNNNNGC 6 cut(s) 278, 386, 443, 512, 554, 902
BstNI CCWGG 1 cut(s) 118
BstNSI RCATGY 1 cut(s) 571
BstSCI CCNGG 2 cut(s) 116, 681
BstSFI CTRYAG 2 cut(s) 135, 282
BstV1I GCAGC 3 cut(s) 125, 268, 455
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 3 cut(s) 132, 272, 515
BtgI CCRYGG 1 cut(s) 573
BtsIMutI CAGTG 2 cut(s) 498, 806
BveI ACCTGC 1 cut(s) 509
Cac8I GCNNGC 1 cut(s) 603
CciI TCATGA 1 cut(s) 702
CfoI GCGC 1 cut(s) 643
Cfr10I RCCGGY 4 cut(s) 104, 314, 674, 833
Cfr13I GGNCC 1 cut(s) 131
Csp6I GTAC 3 cut(s) 538, 678, 823
CspAI ACCGGT 3 cut(s) 104, 674, 833
CviAII CATG 5 cut(s) 79, 268, 568, 637, 703
CviQI GTAC 3 cut(s) 538, 678, 823
DdeI CTNAG 2 cut(s) 181, 198
DinI GGCGCC 1 cut(s) 642
DpnI GATC 4 cut(s) 51, 68, 662, 707
DpnII GATC 4 cut(s) 49, 66, 660, 705
EciI GGCGGA 1 cut(s) 404
Eco32I GATATC 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 116
EcoRV GATATC 1 cut(s) 159
EcoT22I ATGCAT 1 cut(s) 493
EgeI GGCGCC 1 cut(s) 642
EheI GGCGCC 1 cut(s) 642
FaeI CATG 5 cut(s) 82, 271, 571, 640, 706
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FatI CATG 5 cut(s) 78, 267, 567, 636, 702
Fnu4HI GCNGC 4 cut(s) 114, 282, 444, 516
Fsp4HI GCNGC 4 cut(s) 114, 282, 444, 516
FspBI CTAG 2 cut(s) 321, 584
GlaI GCGC 1 cut(s) 642
GluI GCNGC 4 cut(s) 114, 282, 444, 516
GsaI CCCAGC 1 cut(s) 915
GsuI CTGGAG 1 cut(s) 246
HaeII RGCGCY 1 cut(s) 644
HaeIII GGCC 3 cut(s) 132, 272, 515
HapII CCGG 6 cut(s) 105, 218, 315, 675, 683, 834
HhaI GCGC 1 cut(s) 643
Hin1I GRCGYC 1 cut(s) 641
Hin1II CATG 5 cut(s) 82, 271, 571, 640, 706
Hin6I GCGC 1 cut(s) 641
HinP1I GCGC 1 cut(s) 641
HpaII CCGG 6 cut(s) 105, 218, 315, 675, 683, 834
HphI GGTGA 4 cut(s) 94, 119, 272, 848
Hpy166II GTNNAC 1 cut(s) 573
Hpy188I TCNGA 1 cut(s) 626
Hpy188III TCNNGA 3 cut(s) 330, 703, 877
Hpy8I GTNNAC 1 cut(s) 573
HpyAV CCTTC 1 cut(s) 770
HpyCH4III ACNGT 2 cut(s) 502, 577
HpyCH4IV ACGT 1 cut(s) 828
HpyF10VI GCNNNNNNNGC 6 cut(s) 278, 386, 443, 512, 554, 902
HpyF3I CTNAG 2 cut(s) 181, 198
HpySE526I ACGT 1 cut(s) 828
Hsp92I GRCGYC 1 cut(s) 641
Hsp92II CATG 5 cut(s) 82, 271, 571, 640, 706
HspAI GCGC 1 cut(s) 641
KasI GGCGCC 1 cut(s) 640
KpnI GGTACC 1 cut(s) 681
Kzo9I GATC 4 cut(s) 49, 66, 660, 705
LmnI GCTCC 3 cut(s) 211, 253, 554
Lsp1109I GCAGC 3 cut(s) 125, 268, 455
LweI GCATC 5 cut(s) 335, 450, 478, 500, 905
MaeI CTAG 2 cut(s) 321, 584
MaeII ACGT 1 cut(s) 828
MaeIII GTNAC 4 cut(s) 260, 424, 685, 829
MalI GATC 4 cut(s) 51, 68, 662, 707
MboI GATC 4 cut(s) 49, 66, 660, 705
MboII GAAGA 3 cut(s) 40, 639, 724
MfeI CAATTG 2 cut(s) 720, 900
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 2 cut(s) 102, 559
MluCI AATT 5 cut(s) 90, 399, 477, 720, 900
Mly113I GGCGCC 1 cut(s) 641
MnlI CCTC 5 cut(s) 76, 103, 221, 385, 602
Mph1103I ATGCAT 1 cut(s) 493
MseI TTAA 2 cut(s) 480, 561
MspI CCGG 6 cut(s) 105, 218, 315, 675, 683, 834
MspR9I CCNGG 2 cut(s) 118, 683
MunI CAATTG 2 cut(s) 720, 900
MvaI CCWGG 1 cut(s) 118
MwoI GCNNNNNNNGC 6 cut(s) 278, 386, 443, 512, 554, 902
NarI GGCGCC 1 cut(s) 641
NciI CCSGG 1 cut(s) 683
NdeII GATC 4 cut(s) 49, 66, 660, 705
NlaIII CATG 5 cut(s) 82, 271, 571, 640, 706
NlaIV GGNNCC 2 cut(s) 642, 679
NmuCI GTSAC 1 cut(s) 260
NsiI ATGCAT 1 cut(s) 493
NspI RCATGY 1 cut(s) 571
PagI TCATGA 1 cut(s) 702
PflFI GACNNNGTC 1 cut(s) 336
PinAI ACCGGT 3 cut(s) 104, 674, 833
PkrI GCNGC 4 cut(s) 115, 283, 445, 517
PluTI GGCGCC 1 cut(s) 644
Psp6I CCWGG 1 cut(s) 116
PspFI CCCAGC 1 cut(s) 911
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 2 cut(s) 642, 679
PspPI GGNCC 1 cut(s) 131
PstI CTGCAG 2 cut(s) 139, 286
PsuI RGATCY 1 cut(s) 49
PsyI GACNNNGTC 1 cut(s) 336
RsaI GTAC 3 cut(s) 539, 679, 824
RsaNI GTAC 3 cut(s) 538, 678, 823
SaqAI TTAA 2 cut(s) 480, 561
SatI GCNGC 4 cut(s) 114, 282, 444, 516
Sau3AI GATC 4 cut(s) 49, 66, 660, 705
Sau96I GGNCC 1 cut(s) 131
ScrFI CCNGG 2 cut(s) 118, 683
SduI GDGCHC 2 cut(s) 102, 559
SetI ASST 9 cut(s) 166, 216, 258, 469, 523, 594, 762, 831, 895
SfaNI GCATC 5 cut(s) 335, 450, 478, 500, 905
SfcI CTRYAG 2 cut(s) 135, 282
SfoI GGCGCC 1 cut(s) 642
SgrAI CRCCGGYG 1 cut(s) 104
Sse9I AATT 5 cut(s) 90, 399, 477, 720, 900
SsiI CCGC 3 cut(s) 389, 516, 820
SspDI GGCGCC 1 cut(s) 640
SspMI CTAG 2 cut(s) 321, 584
StyD4I CCNGG 2 cut(s) 116, 681
TaaI ACNGT 2 cut(s) 502, 577
TaiI ACGT 1 cut(s) 831
TaqI TCGA 2 cut(s) 69, 607
TasI AATT 5 cut(s) 90, 399, 477, 720, 900
TatI WGTACW 1 cut(s) 537
TauI GCSGC 1 cut(s) 518
Tru1I TTAA 2 cut(s) 480, 561
Tru9I TTAA 2 cut(s) 480, 561
TscAI CASTG 2 cut(s) 505, 813
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 3 cut(s) 113, 281, 443
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 3 cut(s) 41, 256, 539
TspRI CASTG 2 cut(s) 505, 813
Tth111I GACNNNGTC 1 cut(s) 336
XapI RAATTY 1 cut(s) 90
XceI RCATGY 1 cut(s) 571
XspI CTAG 2 cut(s) 321, 584
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.