Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002369

Physical Location & Seq

Genomic Coordinates Reverse (-)

40935075 .. 40941376

Gene Structure 6302 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1274900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1851 bp

ATGAGTATGGGAACGGGCGGCGGAGGAGACGATGAGGGGATACGGCGGAGAGGCTGCACGTACCGAAAGGACGATTTTCTCCCCGAGGAGTCGTTCCAGAGCTGGGGCAATTACGTCGATGCGCTGAAGCAGACGCCGGGCCGGTTCGTGGATCGGGTCCTGACCCGGTCGGCGGACAACACGGAGCTGGTTGAGGTGAAGGCGCGGAGTCATCACGAGATGAAGAAGACGCTCAGCTGGTGGGACCTCATCTGGTTCGGACTCGGCGCTGTCATCGGCGCGGGAATATTCGTGCTCACGGGACTGGAGGCGAGAGAGCACGCGGGCCCCGCCGTCGTGCTGTCGTACGTCGTCTCGGGCGTCTCCGCCTTGCTCTCTGTTTTCTGTTACACCGAGTTCGCCGTTGAGATTCCTGTTGCAGGTGGCTCATTTGCCTACCTAAGGGTGGAGCTTGGGGACTTCGTGGCCTTCATTGCAGCAGGCAACATCCTCCTCGAGTACGTCATAGGTGGCGCAGCCGTTGCTCGTTCTTGGACATCCTATTTCACCACCCTCTGCAATCGCAGCGACACCAATGATTTCCGCATCATAGCCCACGGTTTCCCAGAGGACTACAAGTACCTCGATCCCATTGCTGTCGCTGTCATTATCGGCATCTGCTGCCTCGCAGTCCTCAGCACAAAGGGCTCTTCTCGTCTCAACTACATTGCCTCGATTGTCCACATCATTGTCATTCTCTTCATCATCATCGCTGGCCTCACCAAAGCAGATATCAATAACTACCGCGATTTCTTCCCCTTTGGCCCTCGCGGTGTATTTCAAGCTTCAGCTGTTCTATTCTTTGCGTACATTGGATTTGATGCTGTTTCAACCATGGCGGAGGAGACCAAAAATCCTGCAAGGGACATCCCAATTGGTCTTGTCGGCTCAATGGTGATTACCACAGTCCTCTATTGCTTGCTTGCAGTGACATTATGCCTTATGCGGCCGTATGGCCAAATCGACAAACAAGCTCCATTTTCGGTTGCGTTTGAAGATGTTGGGATGGGATGGGCTAAGTACGTTGTGGCAGCGGGTGCATTGAAAGGCATGACAAGCGTGCTGCTTGTTGGGGCAGTTGGACAGGCGCGGTACCTCACTCACATTGCCCGAACCCACATGGTGCCCCCATGGTTTGCGCAAGTTGATTACAAAACCGGCACTCCTGTCAATGCCACGGTCACCATGCTTGCAGCCACCGCAATCATTGCCTTCTTCACAAGCCTTGACATTCTGGCCAACCTTCTCTCCATCTCCACTCTGTTCATCTTCAGCCTTGTTGCCATCGCTCTCCTTGTTCGCCGCTACTATGTCAGCGGCGTCACAACTCGGGCCAACAGAAACAAGTTCATTGCCTTCCTTTTCATCATAATTAGCTCTTCAATTGCCACCTCTGTCTATTGGAGTAGAACAGATGATTGGACTGCCTACGTGGTAACCGCGCCGCTTTGGTTCTTGGGTACTTTAGGGATTTGGTTACTTGTCCCCCAAGCAAGGAGTCCGAAGCTCTGGGGAGTGCCACTGGTGCCATGGTTGCCATCTCTTTCAATTGCCATCAACATTTTCCTGCTCGGGTCAATCGATCACGCCTCGTTCATAAGGTTTGGAGTGTGGACTCTGATCATCTTGGTTTACTACTTCATCTTCGGATTGCACGCGTCTTATGACACGGCAAAGGACTCCGAAGCGAAGAGGCTGGAGGGCGGCACAAACGATGAGTTGAAGAAGGTTCAAGCTGCAAGGGTGGCCACGAGGGACGCCGGCTTAGACGCTGGAAATGGTAGCAATGCCTCTGCACCTCCTGCTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AA_permease_2 AA_permease_C

Protein Analysis

617

Amino Acids

67.09

Weight (kDa)

7.6

Isoelectric Point (pI)

30.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease_2	PF13520	78 - 471	1.3e-50	Amino acid permease
AA_permease	PF00324	83 - 479	5e-33	Amino acid permease
AA_permease_C	PF13906	517 - 567	7.9e-19	C-terminus of AA_permease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002369)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21120 AT4G21120
fragaria_vesca	FvH4_3g09930 FvH4_3g09940
malus_domestica	MD05G1274600.v1.1 MD05G1274700.v1.1 MD05G1274900.v1.1 MD10G1254300.v1.1
prunus_persica	Prupe.4G089000_v2.0.a1 Prupe.4G089100_v2.0.a1
pyrus_communis	pycom05g25610 pycom05g25630 pycom10g21000
rosa_chinensis	RchiOBHm_Chr5g0015621 RchiOBHm_Chr5g0015661 RchiOBHm_Chr5g0015691 RchiOBHm_Chr5g0015791
rosa_laevigata	RLG00000032214 RLG00000032217 RLG00000032219
rosa_multiflora	Rmu_sc0000843.1_g000004 Rmu_sc0000843.1_g000008 Rmu_sc0003230.1_g000003 Rmu_sc0003230.1_g000005
rosa_roxburghii	Rroxscaffold_1G00060830
rosa_rugosa	Rorug01G0078300 Rorug05G0029000 Rorug05G0029100
rosa_samantha	Rh5AG122300 Rh5AG122400 Rh5AG122600 Rh5BG120900 Rh5BG121200 Rh5BG121700 Rh5CG131300 Rh5CG131900 Rh5CG132800 Rh5DG119900 Rh5DG120600
rosa_wichuraiana	Rw5G010550 Rw5G010560 Rw5G010570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	410
Acc16I	TGCGCA	1 cut(s)	1179
Acc36I	ACCTGC	1 cut(s)	410
Acc65I	GGTACC	1 cut(s)	1131
AccB1I	GGYRCC	3 cut(s)	1131, 1162, 1564
AccII	CGCG	8 cut(s)	205, 281, 323, 786, 810, 1129, 1481, 1697
AclWI	GGATC	2 cut(s)	159, 620
AcoI	YGGCCR	4 cut(s)	986, 994, 1275, 1785
AcuI	CTGAAG	3 cut(s)	146, 810, 1294
AcyI	GRCGYC	4 cut(s)	134, 360, 1359, 1797
AdeI	CACNNNGTG	1 cut(s)	1162
AfaI	GTAC	8 cut(s)	62, 347, 500, 620, 848, 1061, 1133, 1501
AfiI	CCNNNNNNNGG	7 cut(s)	103, 148, 172, 419, 441, 445, 1533
AflIII	ACRYGT	1 cut(s)	1695
AgsI	TTSAA	8 cut(s)	821, 870, 1034, 1084, 1422, 1587, 1762, 1772
AjuI	GAANNNNNNNTTGG	2 cut(s)	1441, 1473
Alw21I	GWGCWC	2 cut(s)	297, 321
Alw26I	GTCTC	5 cut(s)	21, 358, 367, 701, 878
AlwI	GGATC	2 cut(s)	159, 620
Ama87I	CYCGRG	5 cut(s)	83, 355, 494, 1368, 1610
ApaI	GGGCCC	1 cut(s)	329
ApeKI	GCWGC	9 cut(s)	54, 476, 515, 564, 660, 1070, 1102, 1232, 1775
Asp718I	GGTACC	1 cut(s)	1131
AspLEI	GCGC	8 cut(s)	124, 205, 269, 281, 515, 1129, 1180, 1483
AspS9I	GGNCC	7 cut(s)	139, 157, 244, 325, 326, 803, 1371
AsuC2I	CCSGG	2 cut(s)	138, 166
AsuHPI	GGTGA	5 cut(s)	208, 538, 751, 946, 1213
AvaI	CYCGRG	5 cut(s)	83, 355, 494, 1368, 1610
AvaII	GGWCC	2 cut(s)	157, 244
AxyI	CCTNAGG	1 cut(s)	440
BaeGI	GKGCMC	2 cut(s)	329, 1167
BalI	TGGCCA	3 cut(s)	996, 1277, 1787
BanI	GGYRCC	3 cut(s)	1131, 1162, 1564
BanII	GRGCYC	2 cut(s)	329, 689
BauI	CACGAG	2 cut(s)	215, 1789
BbsI	GAAGAC	1 cut(s)	233
Bbv12I	GWGCWC	2 cut(s)	297, 321
BbvCI	CCTCAGC	1 cut(s)	674
BbvI	GCAGC	9 cut(s)	41, 488, 527, 576, 647, 1082, 1089, 1244, 1762
BccI	CCATC	6 cut(s)	1039, 1044, 1298, 1331, 1585, 1601
BceAI	ACGGC	6 cut(s)	59, 317, 386, 503, 973, 1725
BcgI	CGANNNNNNTGC	4 cut(s)	97, 131, 614, 648
BciVI	GTATCC	1 cut(s)	33
BclI	TGATCA	1 cut(s)	1659
BcnI	CCSGG	2 cut(s)	138, 166
BcoDI	GTCTC	5 cut(s)	21, 358, 367, 701, 878
BfaI	CTAG	1 cut(s)	1845
BfoI	RGCGCY	1 cut(s)	270
BfuAI	ACCTGC	1 cut(s)	410
BfuI	GTATCC	1 cut(s)	33
BlpI	GCTNAGC	1 cut(s)	233
Bme1390I	CCNGG	2 cut(s)	138, 166
Bme18I	GGWCC	2 cut(s)	157, 244
BmeT110I	CYCGRG	5 cut(s)	83, 355, 494, 1368, 1610
BmgT120I	GGNCC	7 cut(s)	139, 157, 244, 325, 326, 803, 1371
BmiI	GGNNCC	7 cut(s)	158, 245, 327, 328, 1133, 1164, 1566
BmrFI	CCNGG	2 cut(s)	138, 166
BmsI	GCATC	4 cut(s)	109, 594, 663, 850
BpiI	GAAGAC	1 cut(s)	233
BpmI	CTGGAG	2 cut(s)	326, 1757
Bpu10I	CCTNAGC	1 cut(s)	674
Bpu1102I	GCTNAGC	1 cut(s)	233
BpuMI	CCSGG	2 cut(s)	138, 166
Bsa29I	ATCGAT	1 cut(s)	1620
BsaAI	YACGTR	2 cut(s)	60, 1471
BsaHI	GRCGYC	4 cut(s)	134, 360, 1359, 1797
BsaI	GGTCTC	1 cut(s)	878
BsaJI	CCNNGG	6 cut(s)	84, 595, 873, 1169, 1215, 1568
BsaXI	ACNNNNNCTCC	4 cut(s)	1186, 1216, 1271, 1301
Bsc4I	CCNNNNNNNGG	7 cut(s)	103, 148, 172, 419, 441, 445, 1533
Bse118I	RCCGGY	3 cut(s)	141, 1196, 1799
Bse1I	ACTGG	2 cut(s)	309, 1566
Bse21I	CCTNAGG	1 cut(s)	440
Bse3DI	GCAATG	7 cut(s)	471, 630, 705, 1143, 1245, 1389, 1831
BseCI	ATCGAT	1 cut(s)	1620
BseDI	CCNNGG	6 cut(s)	84, 595, 873, 1169, 1215, 1568
BseGI	GGATG	5 cut(s)	486, 536, 906, 1050, 1055
BseLI	CCNNNNNNNGG	7 cut(s)	103, 148, 172, 419, 441, 445, 1533
BseMI	GCAATG	7 cut(s)	471, 630, 705, 1143, 1245, 1389, 1831
BseMII	CTCAG	2 cut(s)	247, 688
BseNI	ACTGG	2 cut(s)	309, 1566
BseRI	GAGGAG	4 cut(s)	39, 101, 482, 896
BseSI	GKGCMC	2 cut(s)	329, 1167
BseX3I	CGGCCG	1 cut(s)	986
BseXI	GCAGC	9 cut(s)	41, 488, 527, 576, 647, 1082, 1089, 1244, 1762
BseYI	CCCAGC	1 cut(s)	102
BsgI	GTGCAG	2 cut(s)	40, 1818
Bsh1236I	CGCG	8 cut(s)	205, 281, 323, 786, 810, 1129, 1481, 1697
Bsh1285I	CGRYCG	2 cut(s)	170, 989
BshNI	GGYRCC	3 cut(s)	1131, 1162, 1564
BshVI	ATCGAT	1 cut(s)	1620
BsiEI	CGRYCG	2 cut(s)	170, 989
BsiHKAI	GWGCWC	2 cut(s)	297, 321
BsiHKCI	CYCGRG	5 cut(s)	83, 355, 494, 1368, 1610
BsiSI	CCGG	5 cut(s)	137, 142, 166, 1197, 1800
BsiWI	CGTACG	1 cut(s)	345
BslFI	GGGAC	6 cut(s)	257, 315, 470, 917, 1508, 1808
BslI	CCNNNNNNNGG	7 cut(s)	103, 148, 172, 419, 441, 445, 1533
BsmAI	GTCTC	5 cut(s)	21, 358, 367, 701, 878
BsmBI	CGTCTC	4 cut(s)	21, 358, 367, 701
BsmFI	GGGAC	6 cut(s)	257, 315, 470, 917, 1508, 1808
Bso31I	GGTCTC	1 cut(s)	878
BsoBI	CYCGRG	5 cut(s)	83, 355, 494, 1368, 1610
Bsp120I	GGGCCC	1 cut(s)	325
Bsp1286I	GDGCHC	5 cut(s)	297, 321, 329, 689, 1167
Bsp143I	GATC	4 cut(s)	151, 625, 1621, 1659
Bsp1720I	GCTNAGC	1 cut(s)	233
Bsp19I	CCATGG	3 cut(s)	873, 1169, 1568
BspCNI	CTCAG	2 cut(s)	246, 687
BspDI	ATCGAT	1 cut(s)	1620
BspFNI	CGCG	8 cut(s)	205, 281, 323, 786, 810, 1129, 1481, 1697
BspLI	GGNNCC	7 cut(s)	158, 245, 327, 328, 1133, 1164, 1566
BspMI	ACCTGC	1 cut(s)	410
BspPI	GGATC	2 cut(s)	159, 620
BspQI	GCTCTTC	2 cut(s)	694, 1423
BspT107I	GGYRCC	3 cut(s)	1131, 1162, 1564
BspTNI	GGTCTC	1 cut(s)	878
BsrDI	GCAATG	7 cut(s)	471, 630, 705, 1143, 1245, 1389, 1831
BsrFI	RCCGGY	3 cut(s)	141, 1196, 1799
BsrI	ACTGG	2 cut(s)	309, 1566
BssAI	RCCGGY	3 cut(s)	141, 1196, 1799
BssECI	CCNNGG	6 cut(s)	84, 595, 873, 1169, 1215, 1568
BssMI	GATC	4 cut(s)	151, 625, 1621, 1659
BssNI	GRCGYC	4 cut(s)	134, 360, 1359, 1797
BssSI	CACGAG	2 cut(s)	215, 1789
BssT1I	CCWWGG	3 cut(s)	873, 1169, 1568
Bst2BI	CACGAG	2 cut(s)	215, 1789
Bst4CI	ACNGT	3 cut(s)	599, 946, 1219
Bst6I	CTCTTC	4 cut(s)	694, 743, 1423, 1724
BstACI	GRCGYC	4 cut(s)	134, 360, 1359, 1797
BstAPI	GCANNNNNTGC	5 cut(s)	521, 660, 1076, 1247, 1841
BstBAI	YACGTR	2 cut(s)	60, 1471
BstDEI	CTNAG	5 cut(s)	233, 440, 674, 1056, 1804
BstDSI	CCRYGG	5 cut(s)	595, 873, 1169, 1215, 1568
BstEII	GGTNACC	2 cut(s)	1219, 1474
BstENI	CCTNNNNNAGG	2 cut(s)	417, 439
BstF5I	GGATG	5 cut(s)	486, 536, 906, 1050, 1055
BstFNI	CGCG	8 cut(s)	205, 281, 323, 786, 810, 1129, 1481, 1697
BstH2I	RGCGCY	1 cut(s)	270
BstHHI	GCGC	8 cut(s)	124, 205, 269, 281, 515, 1129, 1180, 1483
BstKTI	GATC	4 cut(s)	154, 628, 1624, 1662
BstMAI	GTCTC	5 cut(s)	21, 358, 367, 701, 878
BstMBI	GATC	4 cut(s)	151, 625, 1621, 1659
BstMCI	CGRYCG	2 cut(s)	170, 989
BstPI	GGTNACC	2 cut(s)	1219, 1474
BstSCI	CCNGG	2 cut(s)	136, 164
BstSLI	GKGCMC	2 cut(s)	329, 1167
BstUI	CGCG	8 cut(s)	205, 281, 323, 786, 810, 1129, 1481, 1697
BstV1I	GCAGC	9 cut(s)	41, 488, 527, 576, 647, 1082, 1089, 1244, 1762
BstV2I	GAAGAC	1 cut(s)	233
BstZI	CGGCCG	1 cut(s)	986
Bsu15I	ATCGAT	1 cut(s)	1620
Bsu36I	CCTNAGG	1 cut(s)	440
BsuI	GTATCC	1 cut(s)	33
BsuTUI	ATCGAT	1 cut(s)	1620
BtgI	CCRYGG	5 cut(s)	595, 873, 1169, 1215, 1568
BtgZI	GCGATG	2 cut(s)	733, 1309
BtsCI	GGATG	5 cut(s)	486, 536, 906, 1050, 1055
BtsI	GCAGTG	1 cut(s)	972
BtsIMutI	CAGTG	2 cut(s)	972, 1559
BveI	ACCTGC	1 cut(s)	410
CfoI	GCGC	8 cut(s)	124, 205, 269, 281, 515, 1129, 1180, 1483
Cfr10I	RCCGGY	3 cut(s)	141, 1196, 1799
Cfr13I	GGNCC	7 cut(s)	139, 157, 244, 325, 326, 803, 1371
ClaI	ATCGAT	1 cut(s)	1620
CseI	GACGC	7 cut(s)	142, 238, 349, 1348, 1686, 1805, 1817
Csp6I	GTAC	8 cut(s)	61, 346, 499, 619, 847, 1060, 1132, 1500
CviAII	CATG	6 cut(s)	874, 1090, 1159, 1170, 1225, 1569
CviQI	GTAC	8 cut(s)	61, 346, 499, 619, 847, 1060, 1132, 1500
DdeI	CTNAG	5 cut(s)	233, 440, 674, 1056, 1804
DpnI	GATC	4 cut(s)	153, 627, 1623, 1661
DpnII	GATC	4 cut(s)	151, 625, 1621, 1659
DraIII	CACNNNGTG	1 cut(s)	1162
EaeI	YGGCCR	4 cut(s)	986, 994, 1275, 1785
EagI	CGGCCG	1 cut(s)	986
Eam1104I	CTCTTC	4 cut(s)	694, 743, 1423, 1724
EarI	CTCTTC	4 cut(s)	694, 743, 1423, 1724
EciI	GGCGGA	5 cut(s)	36, 61, 188, 355, 893
EclXI	CGGCCG	1 cut(s)	986
Eco130I	CCWWGG	3 cut(s)	873, 1169, 1568
Eco24I	GRGCYC	2 cut(s)	329, 689
Eco31I	GGTCTC	1 cut(s)	878
Eco32I	GATATC	1 cut(s)	772
Eco47I	GGWCC	2 cut(s)	157, 244
Eco52I	CGGCCG	1 cut(s)	986
Eco57I	CTGAAG	3 cut(s)	146, 810, 1294
Eco81I	CCTNAGG	1 cut(s)	440
Eco88I	CYCGRG	5 cut(s)	83, 355, 494, 1368, 1610
Eco91I	GGTNACC	2 cut(s)	1219, 1474
EcoNI	CCTNNNNNAGG	2 cut(s)	417, 439
EcoO109I	RGGNCCY	3 cut(s)	157, 244, 326
EcoO65I	GGTNACC	2 cut(s)	1219, 1474
EcoRV	GATATC	1 cut(s)	772
EcoT14I	CCWWGG	3 cut(s)	873, 1169, 1568
EcoT38I	GRGCYC	2 cut(s)	329, 689
ErhI	CCWWGG	3 cut(s)	873, 1169, 1568
Esp3I	CGTCTC	4 cut(s)	21, 358, 367, 701
FaeI	CATG	6 cut(s)	877, 1093, 1162, 1173, 1228, 1572
FaqI	GGGAC	6 cut(s)	257, 315, 470, 917, 1508, 1808
FatI	CATG	6 cut(s)	873, 1089, 1158, 1169, 1224, 1568
FauI	CCCGC	4 cut(s)	274, 316, 337, 1066
FbaI	TGATCA	1 cut(s)	1659
FokI	GGATG	5 cut(s)	473, 523, 893, 1057, 1062
FriOI	GRGCYC	2 cut(s)	329, 689
FspBI	CTAG	1 cut(s)	1845
FspI	TGCGCA	1 cut(s)	1179
GlaI	GCGC	8 cut(s)	123, 204, 268, 280, 514, 1128, 1179, 1482
GsaI	CCCAGC	1 cut(s)	106
GsuI	CTGGAG	2 cut(s)	326, 1757
HaeII	RGCGCY	1 cut(s)	270
HapII	CCGG	5 cut(s)	137, 142, 166, 1197, 1800
HgaI	GACGC	7 cut(s)	142, 238, 349, 1348, 1686, 1805, 1817
HhaI	GCGC	8 cut(s)	124, 205, 269, 281, 515, 1129, 1180, 1483
Hin1I	GRCGYC	4 cut(s)	134, 360, 1359, 1797
Hin1II	CATG	6 cut(s)	877, 1093, 1162, 1173, 1228, 1572
Hin6I	GCGC	8 cut(s)	122, 203, 267, 279, 513, 1127, 1178, 1481
HinP1I	GCGC	8 cut(s)	122, 203, 267, 279, 513, 1127, 1178, 1481
HindIII	AAGCTT	1 cut(s)	822
HinfI	GANTC	7 cut(s)	89, 208, 261, 409, 1537, 1654, 1718
HpaII	CCGG	5 cut(s)	137, 142, 166, 1197, 1800
HphI	GGTGA	5 cut(s)	208, 538, 751, 946, 1213
Hpy166II	GTNNAC	3 cut(s)	721, 1653, 1672
Hpy188I	TCNGA	5 cut(s)	260, 1542, 1659, 1688, 1723
Hpy188III	TCNNGA	3 cut(s)	97, 160, 215
Hpy8I	GTNNAC	3 cut(s)	721, 1653, 1672
Hpy99I	CGWCG	3 cut(s)	119, 338, 353
HpyAV	CCTTC	6 cut(s)	193, 478, 1261, 1292, 1405, 1759
HpyCH4III	ACNGT	3 cut(s)	599, 946, 1219
HpyCH4IV	ACGT	6 cut(s)	59, 114, 348, 501, 1062, 1470
HpyF3I	CTNAG	5 cut(s)	233, 440, 674, 1056, 1804
HpySE526I	ACGT	6 cut(s)	59, 114, 348, 501, 1062, 1470
Hsp92I	GRCGYC	4 cut(s)	134, 360, 1359, 1797
Hsp92II	CATG	6 cut(s)	877, 1093, 1162, 1173, 1228, 1572
HspAI	GCGC	8 cut(s)	122, 203, 267, 279, 513, 1127, 1178, 1481
KpnI	GGTACC	1 cut(s)	1135
KroI	GCCGGC	1 cut(s)	1799
KroNI	GCCGGC	1 cut(s)	1801
Ksp22I	TGATCA	1 cut(s)	1659
Kzo9I	GATC	4 cut(s)	151, 625, 1621, 1659
LguI	GCTCTTC	2 cut(s)	694, 1423
LmnI	GCTCC	3 cut(s)	184, 448, 1018
Lsp1109I	GCAGC	9 cut(s)	41, 488, 527, 576, 647, 1082, 1089, 1244, 1762
LweI	GCATC	4 cut(s)	109, 594, 663, 850
MaeI	CTAG	1 cut(s)	1845
MaeII	ACGT	6 cut(s)	59, 114, 348, 501, 1062, 1470
MaeIII	GTNAC	6 cut(s)	386, 967, 1219, 1360, 1474, 1515
MalI	GATC	4 cut(s)	153, 627, 1623, 1661
MboI	GATC	4 cut(s)	151, 625, 1621, 1659
MfeI	CAATTG	3 cut(s)	912, 1422, 1587
MhlI	GDGCHC	5 cut(s)	297, 321, 329, 689, 1167
MlsI	TGGCCA	3 cut(s)	996, 1277, 1787
MluCI	AATT	5 cut(s)	109, 912, 1410, 1422, 1587
MluI	ACGCGT	1 cut(s)	1695
MluNI	TGGCCA	3 cut(s)	996, 1277, 1787
MlyI	GAGTC	6 cut(s)	98, 217, 255, 1546, 1648, 1712
MmeI	TCCRAC	1 cut(s)	1099
Mox20I	TGGCCA	3 cut(s)	996, 1277, 1787
MroNI	GCCGGC	1 cut(s)	1799
MscI	TGGCCA	3 cut(s)	996, 1277, 1787
MseI	TTAA	1 cut(s)	1849
Msp20I	TGGCCA	3 cut(s)	996, 1277, 1787
MspA1I	CMGCKG	4 cut(s)	237, 830, 1073, 1356
MspI	CCGG	5 cut(s)	137, 142, 166, 1197, 1800
MspR9I	CCNGG	2 cut(s)	138, 166
MunI	CAATTG	3 cut(s)	912, 1422, 1587
MvnI	CGCG	8 cut(s)	205, 281, 323, 786, 810, 1129, 1481, 1697
NaeI	GCCGGC	1 cut(s)	1801
NciI	CCSGG	2 cut(s)	138, 166
NcoI	CCATGG	3 cut(s)	873, 1169, 1568
NdeII	GATC	4 cut(s)	151, 625, 1621, 1659
NgoMIV	GCCGGC	1 cut(s)	1799
NlaIII	CATG	6 cut(s)	877, 1093, 1162, 1173, 1228, 1572
NlaIV	GGNNCC	7 cut(s)	158, 245, 327, 328, 1133, 1164, 1566
NmeAIII	GCCGAG	1 cut(s)	243
NmuCI	GTSAC	3 cut(s)	967, 1219, 1360
NsbI	TGCGCA	1 cut(s)	1179
PaeR7I	CTCGAG	1 cut(s)	494
PaqCI	CACCTGC	1 cut(s)	410
PciSI	GCTCTTC	2 cut(s)	694, 1423
PcsI	WCGNNNNNNNCGW	2 cut(s)	357, 1617
PdiI	GCCGGC	1 cut(s)	1801
PfeI	GAWTC	1 cut(s)	409
Pfl23II	CGTACG	1 cut(s)	345
PflFI	GACNNNGTC	1 cut(s)	166
PleI	GAGTC	6 cut(s)	97, 216, 255, 1545, 1648, 1712
PpsI	GAGTC	6 cut(s)	97, 216, 255, 1545, 1648, 1712
Ppu21I	YACGTR	2 cut(s)	60, 1471
PpuMI	RGGWCCY	2 cut(s)	157, 244
Psp5II	RGGWCCY	2 cut(s)	157, 244
PspEI	GGTNACC	2 cut(s)	1219, 1474
PspFI	CCCAGC	1 cut(s)	102
PspLI	CGTACG	1 cut(s)	345
PspN4I	GGNNCC	7 cut(s)	158, 245, 327, 328, 1133, 1164, 1566
PspOMI	GGGCCC	1 cut(s)	325
PspPI	GGNCC	7 cut(s)	139, 157, 244, 325, 326, 803, 1371
PspPPI	RGGWCCY	2 cut(s)	157, 244
PspXI	VCTCGAGB	1 cut(s)	494
PsyI	GACNNNGTC	1 cut(s)	166
PvuII	CAGCTG	2 cut(s)	237, 830
RsaI	GTAC	8 cut(s)	62, 347, 500, 620, 848, 1061, 1133, 1501
RsaNI	GTAC	8 cut(s)	61, 346, 499, 619, 847, 1060, 1132, 1500
SapI	GCTCTTC	2 cut(s)	694, 1423
SaqAI	TTAA	1 cut(s)	1849
Sau3AI	GATC	4 cut(s)	151, 625, 1621, 1659
Sau96I	GGNCC	7 cut(s)	139, 157, 244, 325, 326, 803, 1371
SchI	GAGTC	6 cut(s)	98, 217, 255, 1546, 1648, 1712
ScrFI	CCNGG	2 cut(s)	138, 166
SduI	GDGCHC	5 cut(s)	297, 321, 329, 689, 1167
SfaNI	GCATC	4 cut(s)	109, 594, 663, 850
Sfr274I	CTCGAG	1 cut(s)	494
SinI	GGWCC	2 cut(s)	157, 244
SlaI	CTCGAG	1 cut(s)	494
SmlI	CTYRAG	1 cut(s)	494
SmoI	CTYRAG	1 cut(s)	494
Sse9I	AATT	5 cut(s)	109, 912, 1410, 1422, 1587
SspI	AATATT	1 cut(s)	288
SspMI	CTAG	1 cut(s)	1845
StyD4I	CCNGG	2 cut(s)	136, 164
StyI	CCWWGG	3 cut(s)	873, 1169, 1568
TaaI	ACNGT	3 cut(s)	599, 946, 1219
TaiI	ACGT	6 cut(s)	62, 117, 351, 504, 1065, 1473
TaqI	TCGA	6 cut(s)	117, 495, 624, 713, 1002, 1620
TasI	AATT	5 cut(s)	109, 912, 1410, 1422, 1587
TauI	GCSGC	6 cut(s)	21, 988, 1344, 1359, 1486, 1746
TfiI	GAWTC	1 cut(s)	409
Tru1I	TTAA	1 cut(s)	1849
Tru9I	TTAA	1 cut(s)	1849
TscAI	CASTG	2 cut(s)	972, 1566
TseFI	GTSAC	3 cut(s)	967, 1219, 1360
TseI	GCWGC	9 cut(s)	54, 476, 515, 564, 660, 1070, 1102, 1232, 1775
Tsp45I	GTSAC	3 cut(s)	967, 1219, 1360
TspDTI	ATGAA	8 cut(s)	236, 460, 730, 1294, 1378, 1393, 1624, 1669
TspGWI	ACGGA	1 cut(s)	197
TspRI	CASTG	2 cut(s)	972, 1566
Tth111I	GACNNNGTC	1 cut(s)	166
VpaK11BI	GGWCC	2 cut(s)	157, 244
XagI	CCTNNNNNAGG	2 cut(s)	417, 439
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1566
XhoI	CTCGAG	1 cut(s)	494
XspI	CTAG	1 cut(s)	1845

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.