Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0002369

Physical Location & Seq

Genomic Coordinates Forward (+)

12175853 .. 12188158

Gene Structure 12306 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032217

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1845 bp

ATGGGGATGAGCGGAACGGAACCGAGCGGCGGCGGCGAGGGACTGAAGAGGCGAGGGTGTTCGTGCGGAAAGAACGATTTTCTCCCGGAGGAGTCGTTCGAGAGCTGGGGCAATTACGCCAATGCGCTCCGGTCGACTCCGGCTAGGTTCAAGGACCGGTTCTTCACGCGGTCCGCCTACAGCACCGAGCTGGTGGAGGTGAAGGCGCGGAGCCAGTACGAGATGAAGAAGACGCTTAATTGGTGGGACCTCATCTGGTTCGGGCTCGGAGCCGTCATCGGCGCCGGAATCTTCATCCTCACTGGACTCGAGGCCAAGGAACACGCTGGGCCCTCCGTCGTCATGTCCTACGTCGTCTCCGGCTTCTCCGCCTTGCTCTCTGTTTTCTGTTACACAGAGTTCGCCGTCGAGATTCCCGTTGCAGGCGGCTCATTTGCCTACCTAAGAGTGGAGCTTGGGGACTTCGTGGCCTACATTGCAGCAGGCAACATCCTTCTCGAATATGTCATAGGCGGGGCGGCCGTTGCGCGTTCGTGGACCTCCTACTTCACCTCCCTATGCAACCAGGACGATTCCAATGCTTTCCGAATCACTGTCCACGGTTGGTCGGAGGACTACAAGTACCTGGACCCCATCGCCATTGGGGTTATCATCTTTATCTGCATACTCACCGTCTTGAGCACAAAGGGCTCCTCGCACCTCAACTACGTCGCCTCCATAGTCCATGTCATCGTCATTCTCTTCATCATCATAGCAGGTCTCCTAGCAGGTCTCACCAAAGCAGATACTGACAACTACAAGGAGTTTGCCCCAAATGGAGCTCGCGGCATCTTCCAAGCCTCGGCAGTTCTCTTCTTTGCATACGTTGGATTTGACGCCGTTTCAACCATGGCCGAAGAGACCAAGAATCCTGCTCGAGAGATCCCAATAGGGCTGGTTGGGTCCATGGTGATCACCACCACTCTTTATTGCTTGCTAGCCGTGACACTCTGCATTATGGTACCATATAAGGAAATTGATGTGGACGCTCCATTTTCAAAAGCGTTTGAGACACTTGGTATGGGTTGGGCCAAGTATGTGGTTGCAGCTGGAGCTTTAAAAGGCATGACTAGTGTTTTGCTCGTCGGGGCAGTGGCTCAGGCCCGGTATTTGACCCACATTGCCCGAACCCACATGATGCCCCCATGGTTTGCTCAAGTTGATGCCAAGACAGGTACTCCAGTCCATGCCACAATTGTCGTGTTCGCAGCCACGGCTGTAATCGCTTTCTTCACCAGCCTCAACATTCTTGCTAACCTTCTCTCCATTTCTACGCTATTCATCTTCAGCCTCGTTGCCATTGCTCTACTCGTACGCCGTTACTATGTAACAGGCGTGACCACGGATGCAAACAGGAATAAGCTCATACTGTGCATTGTTATGATACTCGGGGCTGCAATTGCCACTTCTGCTTACTGGGGTGTCAGCCATGATTGGGTAGGGTATTGCGTGACGGCTCCTATTTGGTTCTTGGGGACTTTGGGGATTTGGTTTCTTGTTCCGCAAGCAAGGAGTCCAAAGCTTTGGGGGGTGCCATTAGTTCCATGGTTGCCTTCACTTTCGATTGGCATCAACATTTTCTTGCTTGGTTTGATTGATGGTGCTTCTTTTGTCAGGTTTGCAGTATGGACTGTGATTATCTTGGTTTACTACATTTTCTTTGGGTTGCATGCTTCATATGACACAGCAAAGGACTCTGAAGCTAAGAGGCTCGAGGCTGGGAAATACGAGACGAAGGTTGAAGGAACTAAGGACTCTGGTTTGGATGGTGCGTATAGCAAGGCCTCTACAGAACCTGCTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AA_permease_2 AA_permease_C

Protein Analysis

615

Amino Acids

66.61

Weight (kDa)

6.52

Isoelectric Point (pI)

31.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease_2	PF13520	79 - 488	3.8e-52	Amino acid permease
AA_permease	PF00324	84 - 454	1.1e-35	Amino acid permease
AA_permease_C	PF13906	522 - 572	1.8e-16	C-terminus of AA_permease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002369)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21120 AT4G21120
fragaria_vesca	FvH4_3g09930 FvH4_3g09940
malus_domestica	MD05G1274600.v1.1 MD05G1274700.v1.1 MD05G1274900.v1.1 MD10G1254300.v1.1
prunus_persica	Prupe.4G089000_v2.0.a1 Prupe.4G089100_v2.0.a1
pyrus_communis	pycom05g25610 pycom05g25630 pycom10g21000
rosa_chinensis	RchiOBHm_Chr5g0015621 RchiOBHm_Chr5g0015661 RchiOBHm_Chr5g0015691 RchiOBHm_Chr5g0015791
rosa_laevigata	RLG00000032214 RLG00000032217 RLG00000032219
rosa_multiflora	Rmu_sc0000843.1_g000004 Rmu_sc0000843.1_g000008 Rmu_sc0003230.1_g000003 Rmu_sc0003230.1_g000005
rosa_roxburghii	Rroxscaffold_1G00060830
rosa_rugosa	Rorug01G0078300 Rorug05G0029000 Rorug05G0029100
rosa_samantha	Rh5AG122300 Rh5AG122400 Rh5AG122600 Rh5BG120900 Rh5BG121200 Rh5BG121700 Rh5CG131300 Rh5CG131900 Rh5CG132800 Rh5DG119900 Rh5DG120600
rosa_wichuraiana	Rw5G010550 Rw5G010560 Rw5G010570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	746, 758
Acc65I	GGTACC	1 cut(s)	1000
AccB1I	GGYRCC	3 cut(s)	281, 1000, 1570
AccBSI	CCGCTC	2 cut(s)	12, 27
AccI	GTMKAC	1 cut(s)	134
AccII	CGCG	4 cut(s)	169, 208, 529, 825
AclWI	GGATC	1 cut(s)	916
AcoI	YGGCCR	2 cut(s)	519, 891
AcuI	CTGAAG	3 cut(s)	65, 1309, 1758
AcyI	GRCGYC	2 cut(s)	282, 876
AfaI	GTAC	5 cut(s)	218, 623, 1002, 1216, 1353
AfiI	CCNNNNNNNGG	5 cut(s)	29, 422, 448, 841, 1474
AgeI	ACCGGT	1 cut(s)	156
AgsI	TTSAA	4 cut(s)	151, 885, 1038, 1781
AhlI	ACTAGT	1 cut(s)	1109
AjnI	CCWGG	2 cut(s)	564, 624
AloI	GAACNNNNNNTCC	2 cut(s)	80, 112
AluBI	AGCT	9 cut(s)	105, 190, 454, 821, 1088, 1094, 1402, 1561, 1742
AluI	AGCT	9 cut(s)	105, 190, 454, 821, 1088, 1094, 1402, 1561, 1742
Alw21I	GWGCWC	2 cut(s)	683, 823
Alw26I	GTCTC	6 cut(s)	361, 764, 776, 893, 1043, 1763
AlwI	GGATC	1 cut(s)	916
AlwNI	CAGNNNCTG	2 cut(s)	788, 1835
Ama87I	CYCGRG	4 cut(s)	308, 915, 1427, 1751
AoxI	GGCC	8 cut(s)	312, 329, 468, 519, 891, 1068, 1140, 1821
ApaI	GGGCCC	1 cut(s)	333
ApeKI	GCWGC	4 cut(s)	479, 1085, 1247, 1433
ArsI	GACNNNNNNTTYG	2 cut(s)	1099, 1131
AsiGI	ACCGGT	1 cut(s)	156
Asp718I	GGTACC	1 cut(s)	1000
AspLEI	GCGC	4 cut(s)	127, 208, 284, 529
AsuC2I	CCSGG	2 cut(s)	86, 1144
AsuHPI	GGTGA	7 cut(s)	211, 541, 661, 766, 946, 961, 1264
AsuNHI	GCTAGC	1 cut(s)	976
AvaI	CYCGRG	4 cut(s)	308, 915, 1427, 1751
AvaII	GGWCC	6 cut(s)	154, 171, 247, 537, 628, 942
BaeGI	GKGCMC	1 cut(s)	333
BaeI	ACNNNNGTAYC	2 cut(s)	1206, 1239
BanI	GGYRCC	3 cut(s)	281, 1000, 1570
BanII	GRGCYC	4 cut(s)	267, 333, 692, 823
BbsI	GAAGAC	1 cut(s)	236
Bbv12I	GWGCWC	2 cut(s)	683, 823
BbvI	GCAGC	4 cut(s)	491, 1097, 1259, 1420
BccI	CCATC	3 cut(s)	641, 1631, 1799
BceAI	ACGGC	8 cut(s)	257, 389, 506, 863, 965, 1269, 1341, 1509
BcgI	CGANNNNNNTGC	2 cut(s)	560, 594
BciT130I	CCWGG	2 cut(s)	566, 626
BclI	TGATCA	1 cut(s)	951
BcnI	CCSGG	2 cut(s)	86, 1144
BcoDI	GTCTC	6 cut(s)	361, 764, 776, 893, 1043, 1763
BcuI	ACTAGT	1 cut(s)	1109
BfaI	CTAG	5 cut(s)	144, 764, 977, 1110, 1839
BfmI	CTRYAG	2 cut(s)	178, 1827
BfoI	RGCGCY	1 cut(s)	285
BfuAI	ACCTGC	2 cut(s)	746, 758
Bme1390I	CCNGG	4 cut(s)	86, 566, 626, 1144
Bme18I	GGWCC	6 cut(s)	154, 171, 247, 537, 628, 942
BmeT110I	CYCGRG	4 cut(s)	308, 915, 1427, 1751
BmrFI	CCNGG	4 cut(s)	86, 566, 626, 1144
BmrI	ACTGGG	1 cut(s)	1465
BmsI	GCATC	5 cut(s)	837, 1167, 1192, 1375, 1617
BmtI	GCTAGC	1 cut(s)	980
BmuI	ACTGGG	1 cut(s)	1465
BpiI	GAAGAC	1 cut(s)	236
BpmI	CTGGAG	2 cut(s)	1110, 1203
Bpu10I	CCTNAGC	1 cut(s)	1137
BpuEI	CTTGAG	2 cut(s)	697, 1179
BpuMI	CCSGG	2 cut(s)	86, 1144
BsaBI	GATNNNNATC	1 cut(s)	1607
BsaHI	GRCGYC	2 cut(s)	282, 876
BsaI	GGTCTC	3 cut(s)	764, 776, 893
BsaJI	CCNNGG	9 cut(s)	315, 598, 840, 888, 945, 1184, 1251, 1380, 1583
BsaWI	WCCGGW	2 cut(s)	129, 156
Bsc4I	CCNNNNNNNGG	5 cut(s)	29, 422, 448, 841, 1474
Bse118I	RCCGGY	1 cut(s)	156
Bse1I	ACTGG	4 cut(s)	214, 307, 1220, 1460
Bse3DI	GCAATG	3 cut(s)	474, 1158, 1338
Bse8I	GATNNNNATC	1 cut(s)	1607
BseBI	CCWGG	2 cut(s)	566, 626
BseDI	CCNNGG	9 cut(s)	315, 598, 840, 888, 945, 1184, 1251, 1380, 1583
BseGI	GGATG	5 cut(s)	12, 294, 489, 1390, 1810
BseJI	GATNNNNATC	1 cut(s)	1607
BseLI	CCNNNNNNNGG	5 cut(s)	29, 422, 448, 841, 1474
BseMI	GCAATG	3 cut(s)	474, 1158, 1338
BseMII	CTCAG	1 cut(s)	1151
BseNI	ACTGG	4 cut(s)	214, 307, 1220, 1460
BseRI	GAGGAG	2 cut(s)	104, 682
BseSI	GKGCMC	1 cut(s)	333
BseX3I	CGGCCG	1 cut(s)	519
BseXI	GCAGC	4 cut(s)	491, 1097, 1259, 1420
BseYI	CCCAGC	3 cut(s)	105, 326, 1757
Bsh1236I	CGCG	4 cut(s)	169, 208, 529, 825
Bsh1285I	CGRYCG	2 cut(s)	134, 522
BshFI	GGCC	8 cut(s)	314, 331, 470, 521, 893, 1070, 1142, 1823
BshNI	GGYRCC	3 cut(s)	281, 1000, 1570
BshTI	ACCGGT	1 cut(s)	156
BsiEI	CGRYCG	2 cut(s)	134, 522
BsiHKAI	GWGCWC	2 cut(s)	683, 823
BsiHKCI	CYCGRG	4 cut(s)	308, 915, 1427, 1751
BsiSI	CCGG	7 cut(s)	86, 130, 140, 157, 285, 360, 1144
BsiWI	CGTACG	1 cut(s)	1351
BslFI	GGGAC	4 cut(s)	54, 260, 473, 1528
BslI	CCNNNNNNNGG	5 cut(s)	29, 422, 448, 841, 1474
BsmAI	GTCTC	6 cut(s)	361, 764, 776, 893, 1043, 1763
BsmBI	CGTCTC	2 cut(s)	361, 1763
BsmFI	GGGAC	4 cut(s)	54, 260, 473, 1528
BsnI	GGCC	8 cut(s)	314, 331, 470, 521, 893, 1070, 1142, 1823
Bso31I	GGTCTC	3 cut(s)	764, 776, 893
BsoBI	CYCGRG	4 cut(s)	308, 915, 1427, 1751
Bsp120I	GGGCCC	1 cut(s)	329
Bsp1286I	GDGCHC	5 cut(s)	267, 333, 683, 692, 823
Bsp143I	GATC	2 cut(s)	921, 951
Bsp19I	CCATGG	4 cut(s)	888, 945, 1184, 1583
BspANI	GGCC	8 cut(s)	314, 331, 470, 521, 893, 1070, 1142, 1823
BspCNI	CTCAG	1 cut(s)	1150
BspFNI	CGCG	4 cut(s)	169, 208, 529, 825
BspMI	ACCTGC	2 cut(s)	746, 758
BspOI	GCTAGC	1 cut(s)	980
BspPI	GGATC	1 cut(s)	916
BspT107I	GGYRCC	3 cut(s)	281, 1000, 1570
BspTNI	GGTCTC	3 cut(s)	764, 776, 893
BsrBI	CCGCTC	2 cut(s)	12, 27
BsrDI	GCAATG	3 cut(s)	474, 1158, 1338
BsrFI	RCCGGY	1 cut(s)	156
BsrI	ACTGG	4 cut(s)	214, 307, 1220, 1460
BssAI	RCCGGY	1 cut(s)	156
BssECI	CCNNGG	9 cut(s)	315, 598, 840, 888, 945, 1184, 1251, 1380, 1583
BssMI	GATC	2 cut(s)	921, 951
BssNI	GRCGYC	2 cut(s)	282, 876
BssT1I	CCWWGG	5 cut(s)	315, 888, 945, 1184, 1583
Bst2UI	CCWGG	2 cut(s)	566, 626
Bst4CI	ACNGT	5 cut(s)	595, 602, 673, 1410, 1672
Bst6I	CTCTTC	4 cut(s)	41, 746, 857, 891
BstACI	GRCGYC	2 cut(s)	282, 876
BstC8I	GCNNGC	7 cut(s)	424, 484, 823, 974, 978, 1545, 1710
BstDEI	CTNAG	4 cut(s)	443, 1137, 1743, 1788
BstDSI	CCRYGG	7 cut(s)	598, 888, 945, 1184, 1251, 1380, 1583
BstF5I	GGATG	5 cut(s)	12, 294, 489, 1390, 1810
BstFNI	CGCG	4 cut(s)	169, 208, 529, 825
BstH2I	RGCGCY	1 cut(s)	285
BstHHI	GCGC	4 cut(s)	127, 208, 284, 529
BstKTI	GATC	2 cut(s)	924, 954
BstMAI	GTCTC	6 cut(s)	361, 764, 776, 893, 1043, 1763
BstMBI	GATC	2 cut(s)	921, 951
BstMCI	CGRYCG	2 cut(s)	134, 522
BstMWI	GCNNNNNNNGC	9 cut(s)	33, 476, 524, 687, 1091, 1253, 1262, 1439, 1448
BstNI	CCWGG	2 cut(s)	566, 626
BstNSI	RCATGY	1 cut(s)	1712
BstSCI	CCNGG	4 cut(s)	84, 564, 624, 1142
BstSFI	CTRYAG	2 cut(s)	178, 1827
BstSLI	GKGCMC	1 cut(s)	333
BstUI	CGCG	4 cut(s)	169, 208, 529, 825
BstV1I	GCAGC	4 cut(s)	491, 1097, 1259, 1420
BstV2I	GAAGAC	1 cut(s)	236
BstX2I	RGATCY	1 cut(s)	921
BstXI	CCANNNNNNTGG	2 cut(s)	1078, 1563
BstYI	RGATCY	1 cut(s)	921
BstZI	CGGCCG	1 cut(s)	519
BsuRI	GGCC	8 cut(s)	314, 331, 470, 521, 893, 1070, 1142, 1823
BtgI	CCRYGG	7 cut(s)	598, 888, 945, 1184, 1251, 1380, 1583
BtgZI	GCGATG	1 cut(s)	619
BtsCI	GGATG	5 cut(s)	12, 294, 489, 1390, 1810
BtsI	GCAGTG	1 cut(s)	1137
BtsIMutI	CAGTG	3 cut(s)	300, 591, 1137
BveI	ACCTGC	2 cut(s)	746, 758
Cac8I	GCNNGC	7 cut(s)	424, 484, 823, 974, 978, 1545, 1710
CaiI	CAGNNNCTG	2 cut(s)	788, 1835
CfoI	GCGC	4 cut(s)	127, 208, 284, 529
Cfr10I	RCCGGY	1 cut(s)	156
CpoI	CGGWCCG	1 cut(s)	171
CseI	GACGC	3 cut(s)	241, 884, 1034
Csp6I	GTAC	5 cut(s)	217, 622, 1001, 1215, 1352
CspAI	ACCGGT	1 cut(s)	156
CspI	CGGWCCG	1 cut(s)	171
CviQI	GTAC	5 cut(s)	217, 622, 1001, 1215, 1352
DdeI	CTNAG	4 cut(s)	443, 1137, 1743, 1788
DinI	GGCGCC	1 cut(s)	283
DpnI	GATC	2 cut(s)	923, 953
DpnII	GATC	2 cut(s)	921, 951
DraI	TTTAAA	1 cut(s)	1098
EaeI	YGGCCR	2 cut(s)	519, 891
EagI	CGGCCG	1 cut(s)	519
Eam1104I	CTCTTC	4 cut(s)	41, 746, 857, 891
EarI	CTCTTC	4 cut(s)	41, 746, 857, 891
EciI	GGCGGA	2 cut(s)	163, 358
Ecl136II	GAGCTC	1 cut(s)	821
EclXI	CGGCCG	1 cut(s)	519
Eco130I	CCWWGG	5 cut(s)	315, 888, 945, 1184, 1583
Eco147I	AGGCCT	1 cut(s)	1823
Eco24I	GRGCYC	4 cut(s)	267, 333, 692, 823
Eco31I	GGTCTC	3 cut(s)	764, 776, 893
Eco47I	GGWCC	6 cut(s)	154, 171, 247, 537, 628, 942
Eco52I	CGGCCG	1 cut(s)	519
Eco53kI	GAGCTC	1 cut(s)	821
Eco57I	CTGAAG	3 cut(s)	65, 1309, 1758
Eco88I	CYCGRG	4 cut(s)	308, 915, 1427, 1751
EcoICRI	GAGCTC	1 cut(s)	821
EcoO109I	RGGNCCY	2 cut(s)	247, 330
EcoRII	CCWGG	2 cut(s)	564, 624
EcoT14I	CCWWGG	5 cut(s)	315, 888, 945, 1184, 1583
EcoT38I	GRGCYC	4 cut(s)	267, 333, 692, 823
EgeI	GGCGCC	1 cut(s)	283
EheI	GGCGCC	1 cut(s)	283
ErhI	CCWWGG	5 cut(s)	315, 888, 945, 1184, 1583
Esp3I	CGTCTC	2 cut(s)	361, 1763
FaqI	GGGAC	4 cut(s)	54, 260, 473, 1528
FauI	CCCGC	1 cut(s)	506
FauNDI	CATATG	1 cut(s)	1717
FbaI	TGATCA	1 cut(s)	951
FblI	GTMKAC	1 cut(s)	134
FokI	GGATG	5 cut(s)	19, 281, 476, 1397, 1817
FriOI	GRGCYC	4 cut(s)	267, 333, 692, 823
FspBI	CTAG	5 cut(s)	144, 764, 977, 1110, 1839
GlaI	GCGC	4 cut(s)	126, 207, 283, 528
GsaI	CCCAGC	3 cut(s)	109, 330, 1761
GsuI	CTGGAG	2 cut(s)	1110, 1203
HaeII	RGCGCY	1 cut(s)	285
HaeIII	GGCC	8 cut(s)	314, 331, 470, 521, 893, 1070, 1142, 1823
HapII	CCGG	7 cut(s)	86, 130, 140, 157, 285, 360, 1144
HgaI	GACGC	3 cut(s)	241, 884, 1034
HhaI	GCGC	4 cut(s)	127, 208, 284, 529
Hin1I	GRCGYC	2 cut(s)	282, 876
Hin6I	GCGC	4 cut(s)	125, 206, 282, 527
HinP1I	GCGC	4 cut(s)	125, 206, 282, 527
HincII	GTYRAC	1 cut(s)	135
HindII	GTYRAC	1 cut(s)	135
HindIII	AAGCTT	1 cut(s)	1559
HpaII	CCGG	7 cut(s)	86, 130, 140, 157, 285, 360, 1144
HphI	GGTGA	7 cut(s)	211, 541, 661, 766, 946, 961, 1264
Hpy166II	GTNNAC	5 cut(s)	135, 537, 598, 1024, 1687
Hpy188I	TCNGA	4 cut(s)	269, 587, 610, 1738
Hpy188III	TCNNGA	5 cut(s)	100, 409, 497, 676, 917
Hpy8I	GTNNAC	5 cut(s)	135, 537, 598, 1024, 1687
Hpy99I	CGWCG	5 cut(s)	341, 356, 410, 713, 1127
HpyAV	CCTTC	6 cut(s)	196, 503, 1307, 1602, 1768, 1775
HpyCH4III	ACNGT	5 cut(s)	595, 602, 673, 1410, 1672
HpyCH4IV	ACGT	3 cut(s)	351, 708, 864
HpyF10VI	GCNNNNNNNGC	9 cut(s)	33, 476, 524, 687, 1091, 1253, 1262, 1439, 1448
HpyF3I	CTNAG	4 cut(s)	443, 1137, 1743, 1788
HpySE526I	ACGT	3 cut(s)	351, 708, 864
Hsp92I	GRCGYC	2 cut(s)	282, 876
HspAI	GCGC	4 cut(s)	125, 206, 282, 527
KasI	GGCGCC	1 cut(s)	281
KpnI	GGTACC	1 cut(s)	1004
Ksp22I	TGATCA	1 cut(s)	951
Kzo9I	GATC	2 cut(s)	921, 951
LmnI	GCTCC	9 cut(s)	132, 210, 269, 451, 695, 818, 1033, 1091, 1501
Lsp1109I	GCAGC	4 cut(s)	491, 1097, 1259, 1420
LweI	GCATC	5 cut(s)	837, 1167, 1192, 1375, 1617
MaeI	CTAG	5 cut(s)	144, 764, 977, 1110, 1839
MaeII	ACGT	3 cut(s)	351, 708, 864
MaeIII	GTNAC	6 cut(s)	389, 982, 1358, 1366, 1375, 1489
MalI	GATC	2 cut(s)	923, 953
MbiI	CCGCTC	2 cut(s)	12, 27
MboI	GATC	2 cut(s)	921, 951
MfeI	CAATTG	2 cut(s)	1233, 1437
MflI	RGATCY	1 cut(s)	921
MhlI	GDGCHC	5 cut(s)	267, 333, 683, 692, 823
MluCI	AATT	5 cut(s)	112, 238, 1014, 1233, 1437
Mly113I	GGCGCC	1 cut(s)	282
MlyI	GAGTC	6 cut(s)	101, 130, 300, 1561, 1727, 1787
MmeI	TCCRAC	2 cut(s)	588, 847
MseI	TTAA	3 cut(s)	237, 1097, 1843
MslI	CAYNNNNRTG	1 cut(s)	1418
MspA1I	CMGCKG	1 cut(s)	1088
MspI	CCGG	7 cut(s)	86, 130, 140, 157, 285, 360, 1144
MspR9I	CCNGG	4 cut(s)	86, 566, 626, 1144
MunI	CAATTG	2 cut(s)	1233, 1437
MvaI	CCWGG	2 cut(s)	566, 626
MvnI	CGCG	4 cut(s)	169, 208, 529, 825
MwoI	GCNNNNNNNGC	9 cut(s)	33, 476, 524, 687, 1091, 1253, 1262, 1439, 1448
NarI	GGCGCC	1 cut(s)	282
NciI	CCSGG	2 cut(s)	86, 1144
NcoI	CCATGG	4 cut(s)	888, 945, 1184, 1583
NdeI	CATATG	1 cut(s)	1717
NdeII	GATC	2 cut(s)	921, 951
NheI	GCTAGC	1 cut(s)	976
NmeAIII	GCCGAG	1 cut(s)	821
NmuCI	GTSAC	3 cut(s)	982, 1375, 1489
NspI	RCATGY	1 cut(s)	1712
PaeI	GCATGC	1 cut(s)	1712
PaeR7I	CTCGAG	3 cut(s)	308, 915, 1751
PceI	AGGCCT	1 cut(s)	1823
PcsI	WCGNNNNNNNCGW	1 cut(s)	414
PfeI	GAWTC	5 cut(s)	288, 412, 572, 588, 907
Pfl23II	CGTACG	1 cut(s)	1351
PfoI	TCCNGGA	1 cut(s)	84
PinAI	ACCGGT	1 cut(s)	156
PleI	GAGTC	6 cut(s)	100, 130, 300, 1560, 1727, 1787
PluTI	GGCGCC	1 cut(s)	285
PpsI	GAGTC	6 cut(s)	100, 130, 300, 1560, 1727, 1787
PpuMI	RGGWCCY	1 cut(s)	247
Psp124BI	GAGCTC	1 cut(s)	823
Psp5II	RGGWCCY	1 cut(s)	247
Psp6I	CCWGG	2 cut(s)	564, 624
PspFI	CCCAGC	3 cut(s)	105, 326, 1757
PspGI	CCWGG	2 cut(s)	564, 624
PspLI	CGTACG	1 cut(s)	1351
PspOMI	GGGCCC	1 cut(s)	329
PspPPI	RGGWCCY	1 cut(s)	247
PspXI	VCTCGAGB	2 cut(s)	308, 1751
PstNI	CAGNNNCTG	2 cut(s)	788, 1835
PsuI	RGATCY	1 cut(s)	921
PvuII	CAGCTG	1 cut(s)	1088
RsaI	GTAC	5 cut(s)	218, 623, 1002, 1216, 1353
RsaNI	GTAC	5 cut(s)	217, 622, 1001, 1215, 1352
RseI	CAYNNNNRTG	1 cut(s)	1418
Rsr2I	CGGWCCG	1 cut(s)	171
RsrII	CGGWCCG	1 cut(s)	171
SacI	GAGCTC	1 cut(s)	823
SalI	GTCGAC	1 cut(s)	133
SaqAI	TTAA	3 cut(s)	237, 1097, 1843
Sau3AI	GATC	2 cut(s)	921, 951
SchI	GAGTC	6 cut(s)	101, 130, 300, 1561, 1727, 1787
ScrFI	CCNGG	4 cut(s)	86, 566, 626, 1144
SduI	GDGCHC	5 cut(s)	267, 333, 683, 692, 823
SfaNI	GCATC	5 cut(s)	837, 1167, 1192, 1375, 1617
SfcI	CTRYAG	2 cut(s)	178, 1827
SfoI	GGCGCC	1 cut(s)	283
Sfr274I	CTCGAG	3 cut(s)	308, 915, 1751
SinI	GGWCC	6 cut(s)	154, 171, 247, 537, 628, 942
SlaI	CTCGAG	3 cut(s)	308, 915, 1751
SmiMI	CAYNNNNRTG	1 cut(s)	1418
SmlI	CTYRAG	5 cut(s)	308, 676, 915, 1194, 1751
SmoI	CTYRAG	5 cut(s)	308, 676, 915, 1194, 1751
SpeI	ACTAGT	1 cut(s)	1109
SphI	GCATGC	1 cut(s)	1712
Sse9I	AATT	5 cut(s)	112, 238, 1014, 1233, 1437
SseBI	AGGCCT	1 cut(s)	1823
SspDI	GGCGCC	1 cut(s)	281
SspMI	CTAG	5 cut(s)	144, 764, 977, 1110, 1839
SstI	GAGCTC	1 cut(s)	823
StuI	AGGCCT	1 cut(s)	1823
StyD4I	CCNGG	4 cut(s)	84, 564, 624, 1142
StyI	CCWWGG	5 cut(s)	315, 888, 945, 1184, 1583
TaaI	ACNGT	5 cut(s)	595, 602, 673, 1410, 1672
TaiI	ACGT	3 cut(s)	354, 711, 867
TaqI	TCGA	8 cut(s)	99, 134, 309, 408, 498, 916, 1601, 1752
TasI	AATT	5 cut(s)	112, 238, 1014, 1233, 1437
TauI	GCSGC	6 cut(s)	30, 33, 36, 429, 521, 828
TfiI	GAWTC	5 cut(s)	288, 412, 572, 588, 907
Tru1I	TTAA	3 cut(s)	237, 1097, 1843
Tru9I	TTAA	3 cut(s)	237, 1097, 1843
TscAI	CASTG	3 cut(s)	307, 598, 1137
TseFI	GTSAC	3 cut(s)	982, 1375, 1489
TseI	GCWGC	4 cut(s)	479, 1085, 1247, 1433
Tsp45I	GTSAC	3 cut(s)	982, 1375, 1489
TspDTI	ATGAA	5 cut(s)	239, 283, 733, 1309, 1704
TspGWI	ACGGA	3 cut(s)	32, 325, 1397
TspRI	CASTG	3 cut(s)	307, 598, 1137
VpaK11BI	GGWCC	6 cut(s)	154, 171, 247, 537, 628, 942
XceI	RCATGY	1 cut(s)	1712
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1581
XhoI	CTCGAG	3 cut(s)	308, 915, 1751
XmiI	GTMKAC	1 cut(s)	134
XspI	CTAG	5 cut(s)	144, 764, 977, 1110, 1839

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.