Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002369

Physical Location & Seq

Genomic Coordinates Reverse (-)

34670651 .. 34675159

Gene Structure 4509 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1254300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1845 bp

ATGGTGGCGGGCGGCGGAGGAGACGATGAGGGGATACGGCGGAGAGGCTGCACGTACCGAAGGAACGATTTTCTCCCCGAGGAGTCGTTCCAGAGCTGGTGGAATTACGTCCATGCGCTGTGCCAGACGCCGGGCCGGTTCGTGGATCGGGTCCTGACCCGGTCGGCGGACAGCACGGAGCTGGTGGAGATGAAGGCGCGGAGTCATAACGAGATGAAGAAGACGCTCAACTGGTGGGACCTCATCTGGTTCGGACTCGGCGCTGTCATCGGCGCCGGAATATTCGTGCTCACCGGACTGGAGGCGAAAAACCACGCGGGCCCCGCCGTCGTGCTGTCGTACGTCGTCTCGGGCGTCTCCGCCTTGCTCTCTGTTTTCTGCTACACCGAGTTCGCAGTCGAGATTCCTGTTGCAGGGGGCTCATTTGCCTACCTAAGGGTGGAGCTAGGTGACTTCGTGGCCTTCATTGCAGCAGGCAACATCCTCCTCGAGTATGTCATAGGCGGCGCAGCCGTTGCTCGTTCCTGGACCTCCTACTTCACCACCCTCTGCAACCGCAGCGACACCAATGATTTCCGCATCGTAGCCCACGATTTGCCAGAGGACTACAGGTTCCTCGACCCCATTGCCGTTGTTGTCATTATCGGCATCTGCTGCCTCGCAGTCCTCAGCACAAAGGGCTCTTCTCGTCTCAACTACATTGCCTCGATTGTCCATGTAATTGTCATTATCTTCATCATCATTGCCGGCCTCACCAAAGCTGATACCAAAAACTACATCGATTTCGCCCCCTTTGGCCCCCGCGGTGTGTTCCAAGCCTCAGCTGTACTATTCTTCGCCTACGTTGGATTCGATGCTGTTTCAACCATGGCGGAGGAGACCAAGAATCCCGCAAGGGACATCCCAATTGGTCTTGTCGGTTCAATGGTGATTACCACAGTGGTCTATTGCTTGCTTGCAGTGACATTATGCCTTATGCAGCCGTATGGCAGCATTGACGAACAAGCTCCATTTTCGGTTGCATTTGAAGCTGTTGGGATGGGATGGGCTAAGTACATTGTGGCAGCGGGCGCATTGAAAGGTATGACAAGCGTGCTTCTTGTCGGGGCAGTTGGACAGGCTCGGTACCTCACTCACATTGCCCGAACCCACATGGTGCCGCCATGGTTTGCGCAAGTTGATTCCAAAACCGGCACTCCTGTCAATGCGACGGTCACCATGCTTGCAGCCACCGCAATCATTGCCTTCTTCTCAAGCCTCGACGTTCTGGCCAACCTTCTCTCCATCTCCACTCTGTTCATCTTCAGCCTTGTTGCCATGGCTCTCCTTGTTCGCCGCTACTATGTAAGCGGCGTCACAACTCAGGCCAACAGGAACAAGTTCATTGCCTTCCTTTTCATCATAATTACCTCTTCAATTGCCACCTCTATCTACTGGAGCAGAACAGATAAATGGACTGCCTACGTGGTAACCGCGCCGCTTTGGTTCTTGGGTACTTTCGGGATTTGGTTACTTGTCCCCCAAGCAAGGGATCCGAAGCTCTGGGGAGTGCCACTGGTGCCATGGTTGCCATCTCTTTCAATTGCCATCAACATTTTCCTGCTCGGGTCAATCGATCACGCCTCATTCGTAAGGTTTGGAGTGTGGACTGTGATCATCTTGGTTTACTACTTCTTCTTCGGGTTGCACGCGTCTTATGACACGGCAAAGGACTCCGAAGCGAAGAGGCTGGAGGGCGGCACAGGCAATGAGTTGTGGAAGGTTGAAGCTGCAGGGGTTGCGACAAGGGACCCCGGATTAGGCAGTGGAAATGGTAGCTATGCCTCTGCAACTGCTGCTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AA_permease_2 AA_permease_C

Protein Analysis

615

Amino Acids

66.73

Weight (kDa)

6.76

Isoelectric Point (pI)

29.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease_2	PF13520	76 - 452	7.3e-49	Amino acid permease
AA_permease	PF00324	81 - 478	2.4e-33	Amino acid permease
AA_permease_C	PF13906	515 - 565	6.4e-19	C-terminus of AA_permease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002369)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21120 AT4G21120
fragaria_vesca	FvH4_3g09930 FvH4_3g09940
malus_domestica	MD05G1274600.v1.1 MD05G1274700.v1.1 MD05G1274900.v1.1 MD10G1254300.v1.1
prunus_persica	Prupe.4G089000_v2.0.a1 Prupe.4G089100_v2.0.a1
pyrus_communis	pycom05g25610 pycom05g25630 pycom10g21000
rosa_chinensis	RchiOBHm_Chr5g0015621 RchiOBHm_Chr5g0015661 RchiOBHm_Chr5g0015691 RchiOBHm_Chr5g0015791
rosa_laevigata	RLG00000032214 RLG00000032217 RLG00000032219
rosa_multiflora	Rmu_sc0000843.1_g000004 Rmu_sc0000843.1_g000008 Rmu_sc0003230.1_g000003 Rmu_sc0003230.1_g000005
rosa_roxburghii	Rroxscaffold_1G00060830
rosa_rugosa	Rorug01G0078300 Rorug05G0029000 Rorug05G0029100
rosa_samantha	Rh5AG122300 Rh5AG122400 Rh5AG122600 Rh5BG120900 Rh5BG121200 Rh5BG121700 Rh5CG131300 Rh5CG131900 Rh5CG132800 Rh5DG119900 Rh5DG120600
rosa_wichuraiana	Rw5G010550 Rw5G010560 Rw5G010570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1173
Acc65I	GGTACC	1 cut(s)	1125
AccB1I	GGYRCC	4 cut(s)	272, 1125, 1156, 1558
AccII	CGCG	5 cut(s)	199, 317, 804, 1475, 1691
AclWI	GGATC	3 cut(s)	153, 1526, 1539
AcoI	YGGCCR	1 cut(s)	1269
AcuI	CTGAAG	1 cut(s)	1288
AcyI	GRCGYC	4 cut(s)	128, 273, 354, 1353
AdeI	CACNNNGTG	1 cut(s)	1156
AfaI	GTAC	6 cut(s)	56, 341, 828, 1055, 1127, 1495
AfiI	CCNNNNNNNGG	9 cut(s)	130, 142, 166, 413, 435, 439, 1527, 1528, 1799
AflIII	ACRYGT	1 cut(s)	1689
AgsI	TTSAA	7 cut(s)	864, 924, 1028, 1078, 1416, 1581, 1766
AjnI	CCWGG	1 cut(s)	524
AloI	GAACNNNNNNTCC	2 cut(s)	596, 628
Alw21I	GWGCWC	1 cut(s)	291
Alw26I	GTCTC	5 cut(s)	15, 352, 361, 695, 872
AlwI	GGATC	3 cut(s)	153, 1526, 1539
Ama87I	CYCGRG	4 cut(s)	77, 349, 488, 1604
AoxI	GGCC	7 cut(s)	133, 319, 459, 748, 796, 1269, 1365
ApaI	GGGCCC	1 cut(s)	323
Asp700I	GAANNNNTTC	1 cut(s)	1379
Asp718I	GGTACC	1 cut(s)	1125
AspLEI	GCGC	8 cut(s)	118, 199, 263, 275, 509, 1073, 1174, 1477
AspS9I	GGNCC	8 cut(s)	133, 151, 238, 319, 320, 528, 797, 1789
AsuC2I	CCSGG	3 cut(s)	132, 160, 1794
AsuHPI	GGTGA	6 cut(s)	283, 461, 532, 745, 940, 1207
AvaI	CYCGRG	4 cut(s)	77, 349, 488, 1604
AvaII	GGWCC	4 cut(s)	151, 238, 528, 1789
AxyI	CCTNAGG	1 cut(s)	434
BaeGI	GKGCMC	1 cut(s)	323
BalI	TGGCCA	1 cut(s)	1271
BamHI	GGATCC	1 cut(s)	1531
BanI	GGYRCC	4 cut(s)	272, 1125, 1156, 1558
BanII	GRGCYC	3 cut(s)	323, 422, 683
BbsI	GAAGAC	1 cut(s)	227
Bbv12I	GWGCWC	1 cut(s)	291
BbvCI	CCTCAGC	2 cut(s)	668, 820
BccI	CCATC	5 cut(s)	1033, 1038, 1292, 1579, 1595
BceAI	ACGGC	6 cut(s)	53, 311, 497, 614, 967, 1719
BcgI	CGANNNNNNTGC	2 cut(s)	608, 642
BciT130I	CCWGG	1 cut(s)	526
BciVI	GTATCC	1 cut(s)	27
BclI	TGATCA	1 cut(s)	1653
BcnI	CCSGG	3 cut(s)	132, 160, 1794
BcoDI	GTCTC	5 cut(s)	15, 352, 361, 695, 872
BfaI	CTAG	1 cut(s)	446
BfmI	CTRYAG	2 cut(s)	607, 1770
BfoI	RGCGCY	2 cut(s)	264, 276
BfuI	GTATCC	1 cut(s)	27
Bme1390I	CCNGG	4 cut(s)	132, 160, 526, 1794
Bme18I	GGWCC	4 cut(s)	151, 238, 528, 1789
BmeT110I	CYCGRG	4 cut(s)	77, 349, 488, 1604
BmgT120I	GGNCC	8 cut(s)	133, 151, 238, 319, 320, 528, 797, 1789
BmrFI	CCNGG	4 cut(s)	132, 160, 526, 1794
BmsI	GCATC	3 cut(s)	588, 657, 844
BpiI	GAAGAC	1 cut(s)	227
BpmI	CTGGAG	3 cut(s)	320, 1456, 1751
Bpu10I	CCTNAGC	2 cut(s)	668, 820
BpuEI	CTTGAG	1 cut(s)	1237
BpuMI	CCSGG	3 cut(s)	132, 160, 1794
Bsa29I	ATCGAT	2 cut(s)	780, 1614
BsaAI	YACGTR	2 cut(s)	54, 1465
BsaHI	GRCGYC	4 cut(s)	128, 273, 354, 1353
BsaI	GGTCTC	1 cut(s)	872
BsaJI	CCNNGG	7 cut(s)	78, 802, 867, 1163, 1317, 1562, 1792
BsaWI	WCCGGW	1 cut(s)	293
BsaXI	ACNNNNNCTCC	4 cut(s)	1180, 1210, 1265, 1295
Bsc4I	CCNNNNNNNGG	9 cut(s)	130, 142, 166, 413, 435, 439, 1527, 1528, 1799
Bse118I	RCCGGY	3 cut(s)	135, 746, 1190
Bse1I	ACTGG	4 cut(s)	236, 303, 1439, 1560
Bse21I	CCTNAGG	1 cut(s)	434
Bse3DI	GCAATG	8 cut(s)	465, 624, 699, 741, 1137, 1239, 1383, 1753
BseBI	CCWGG	1 cut(s)	526
BseCI	ATCGAT	2 cut(s)	780, 1614
BseDI	CCNNGG	7 cut(s)	78, 802, 867, 1163, 1317, 1562, 1792
BseGI	GGATG	4 cut(s)	480, 900, 1044, 1049
BseLI	CCNNNNNNNGG	9 cut(s)	130, 142, 166, 413, 435, 439, 1527, 1528, 1799
BseMI	GCAATG	8 cut(s)	465, 624, 699, 741, 1137, 1239, 1383, 1753
BseMII	CTCAG	3 cut(s)	682, 834, 1376
BseNI	ACTGG	4 cut(s)	236, 303, 1439, 1560
BseRI	GAGGAG	4 cut(s)	33, 95, 476, 890
BseSI	GKGCMC	1 cut(s)	323
BsgI	GTGCAG	1 cut(s)	34
Bsh1236I	CGCG	5 cut(s)	199, 317, 804, 1475, 1691
Bsh1285I	CGRYCG	1 cut(s)	164
BshFI	GGCC	7 cut(s)	135, 321, 461, 750, 798, 1271, 1367
BshNI	GGYRCC	4 cut(s)	272, 1125, 1156, 1558
BshVI	ATCGAT	2 cut(s)	780, 1614
BsiEI	CGRYCG	1 cut(s)	164
BsiHKAI	GWGCWC	1 cut(s)	291
BsiHKCI	CYCGRG	4 cut(s)	77, 349, 488, 1604
BsiSI	CCGG	8 cut(s)	131, 136, 160, 276, 294, 747, 1191, 1794
BsiWI	CGTACG	1 cut(s)	339
BslFI	GGGAC	4 cut(s)	251, 911, 1502, 1802
BslI	CCNNNNNNNGG	9 cut(s)	130, 142, 166, 413, 435, 439, 1527, 1528, 1799
BsmAI	GTCTC	5 cut(s)	15, 352, 361, 695, 872
BsmBI	CGTCTC	4 cut(s)	15, 352, 361, 695
BsmFI	GGGAC	4 cut(s)	251, 911, 1502, 1802
BsnI	GGCC	7 cut(s)	135, 321, 461, 750, 798, 1271, 1367
Bso31I	GGTCTC	1 cut(s)	872
BsoBI	CYCGRG	4 cut(s)	77, 349, 488, 1604
Bsp120I	GGGCCC	1 cut(s)	319
Bsp1286I	GDGCHC	4 cut(s)	291, 323, 422, 683
Bsp143I	GATC	4 cut(s)	145, 1531, 1615, 1653
Bsp19I	CCATGG	4 cut(s)	867, 1163, 1317, 1562
BspANI	GGCC	7 cut(s)	135, 321, 461, 750, 798, 1271, 1367
BspCNI	CTCAG	3 cut(s)	681, 833, 1375
BspDI	ATCGAT	2 cut(s)	780, 1614
BspFNI	CGCG	5 cut(s)	199, 317, 804, 1475, 1691
BspMAI	CTGCAG	1 cut(s)	1774
BspPI	GGATC	3 cut(s)	153, 1526, 1539
BspQI	GCTCTTC	1 cut(s)	688
BspT107I	GGYRCC	4 cut(s)	272, 1125, 1156, 1558
BspTNI	GGTCTC	1 cut(s)	872
BsrDI	GCAATG	8 cut(s)	465, 624, 699, 741, 1137, 1239, 1383, 1753
BsrFI	RCCGGY	3 cut(s)	135, 746, 1190
BsrI	ACTGG	4 cut(s)	236, 303, 1439, 1560
BssAI	RCCGGY	3 cut(s)	135, 746, 1190
BssECI	CCNNGG	7 cut(s)	78, 802, 867, 1163, 1317, 1562, 1792
BssMI	GATC	4 cut(s)	145, 1531, 1615, 1653
BssNI	GRCGYC	4 cut(s)	128, 273, 354, 1353
BssT1I	CCWWGG	4 cut(s)	867, 1163, 1317, 1562
Bst2UI	CCWGG	1 cut(s)	526
Bst4CI	ACNGT	3 cut(s)	940, 1213, 1651
Bst6I	CTCTTC	3 cut(s)	688, 1417, 1718
BstACI	GRCGYC	4 cut(s)	128, 273, 354, 1353
BstAPI	GCANNNNNTGC	5 cut(s)	515, 654, 1241, 1778, 1835
BstBAI	YACGTR	2 cut(s)	54, 1465
BstDEI	CTNAG	5 cut(s)	434, 668, 820, 1050, 1362
BstDSI	CCRYGG	5 cut(s)	802, 867, 1163, 1317, 1562
BstEII	GGTNACC	2 cut(s)	1213, 1468
BstENI	CCTNNNNNAGG	2 cut(s)	411, 433
BstF5I	GGATG	4 cut(s)	480, 900, 1044, 1049
BstFNI	CGCG	5 cut(s)	199, 317, 804, 1475, 1691
BstH2I	RGCGCY	2 cut(s)	264, 276
BstHHI	GCGC	8 cut(s)	118, 199, 263, 275, 509, 1073, 1174, 1477
BstKTI	GATC	4 cut(s)	148, 1534, 1618, 1656
BstMAI	GTCTC	5 cut(s)	15, 352, 361, 695, 872
BstMBI	GATC	4 cut(s)	145, 1531, 1615, 1653
BstMCI	CGRYCG	1 cut(s)	164
BstNI	CCWGG	1 cut(s)	526
BstPI	GGTNACC	2 cut(s)	1213, 1468
BstSCI	CCNGG	4 cut(s)	130, 158, 524, 1792
BstSFI	CTRYAG	2 cut(s)	607, 1770
BstSLI	GKGCMC	1 cut(s)	323
BstUI	CGCG	5 cut(s)	199, 317, 804, 1475, 1691
BstV2I	GAAGAC	1 cut(s)	227
BstX2I	RGATCY	1 cut(s)	1531
BstYI	RGATCY	1 cut(s)	1531
Bsu15I	ATCGAT	2 cut(s)	780, 1614
Bsu36I	CCTNAGG	1 cut(s)	434
BsuI	GTATCC	1 cut(s)	27
BsuRI	GGCC	7 cut(s)	135, 321, 461, 750, 798, 1271, 1367
BsuTUI	ATCGAT	2 cut(s)	780, 1614
BtgI	CCRYGG	5 cut(s)	802, 867, 1163, 1317, 1562
BtsCI	GGATG	4 cut(s)	480, 900, 1044, 1049
BtsI	GCAGTG	2 cut(s)	966, 1810
BtsIMutI	CAGTG	4 cut(s)	945, 966, 1553, 1810
CfoI	GCGC	8 cut(s)	118, 199, 263, 275, 509, 1073, 1174, 1477
Cfr10I	RCCGGY	3 cut(s)	135, 746, 1190
Cfr13I	GGNCC	8 cut(s)	133, 151, 238, 319, 320, 528, 797, 1789
Cfr42I	CCGCGG	1 cut(s)	805
ClaI	ATCGAT	2 cut(s)	780, 1614
CseI	GACGC	5 cut(s)	136, 232, 343, 1342, 1680
Csp6I	GTAC	6 cut(s)	55, 340, 827, 1054, 1126, 1494
CviAII	CATG	8 cut(s)	113, 716, 868, 1153, 1164, 1219, 1318, 1563
CviQI	GTAC	6 cut(s)	55, 340, 827, 1054, 1126, 1494
DdeI	CTNAG	5 cut(s)	434, 668, 820, 1050, 1362
DinI	GGCGCC	1 cut(s)	274
DpnI	GATC	4 cut(s)	147, 1533, 1617, 1655
DpnII	GATC	4 cut(s)	145, 1531, 1615, 1653
DraIII	CACNNNGTG	1 cut(s)	1156
EaeI	YGGCCR	1 cut(s)	1269
Eam1104I	CTCTTC	3 cut(s)	688, 1417, 1718
EarI	CTCTTC	3 cut(s)	688, 1417, 1718
EciI	GGCGGA	5 cut(s)	30, 55, 182, 349, 887
Eco130I	CCWWGG	4 cut(s)	867, 1163, 1317, 1562
Eco24I	GRGCYC	3 cut(s)	323, 422, 683
Eco31I	GGTCTC	1 cut(s)	872
Eco47I	GGWCC	4 cut(s)	151, 238, 528, 1789
Eco57I	CTGAAG	1 cut(s)	1288
Eco81I	CCTNAGG	1 cut(s)	434
Eco88I	CYCGRG	4 cut(s)	77, 349, 488, 1604
Eco91I	GGTNACC	2 cut(s)	1213, 1468
EcoNI	CCTNNNNNAGG	2 cut(s)	411, 433
EcoO109I	RGGNCCY	4 cut(s)	151, 238, 320, 1789
EcoO65I	GGTNACC	2 cut(s)	1213, 1468
EcoRII	CCWGG	1 cut(s)	524
EcoT14I	CCWWGG	4 cut(s)	867, 1163, 1317, 1562
EcoT38I	GRGCYC	3 cut(s)	323, 422, 683
EgeI	GGCGCC	1 cut(s)	274
EheI	GGCGCC	1 cut(s)	274
ErhI	CCWWGG	4 cut(s)	867, 1163, 1317, 1562
Esp3I	CGTCTC	4 cut(s)	15, 352, 361, 695
FaeI	CATG	8 cut(s)	116, 719, 871, 1156, 1167, 1222, 1321, 1566
FaqI	GGGAC	4 cut(s)	251, 911, 1502, 1802
FatI	CATG	8 cut(s)	112, 715, 867, 1152, 1163, 1218, 1317, 1562
FauI	CCCGC	5 cut(s)	310, 331, 809, 898, 1060
FbaI	TGATCA	1 cut(s)	1653
FokI	GGATG	4 cut(s)	467, 887, 1051, 1056
FriOI	GRGCYC	3 cut(s)	323, 422, 683
FspBI	CTAG	1 cut(s)	446
FspI	TGCGCA	1 cut(s)	1173
GlaI	GCGC	8 cut(s)	117, 198, 262, 274, 508, 1072, 1173, 1476
GsuI	CTGGAG	3 cut(s)	320, 1456, 1751
HaeII	RGCGCY	2 cut(s)	264, 276
HaeIII	GGCC	7 cut(s)	135, 321, 461, 750, 798, 1271, 1367
HapII	CCGG	8 cut(s)	131, 136, 160, 276, 294, 747, 1191, 1794
HgaI	GACGC	5 cut(s)	136, 232, 343, 1342, 1680
HhaI	GCGC	8 cut(s)	118, 199, 263, 275, 509, 1073, 1174, 1477
Hin1I	GRCGYC	4 cut(s)	128, 273, 354, 1353
Hin1II	CATG	8 cut(s)	116, 719, 871, 1156, 1167, 1222, 1321, 1566
Hin6I	GCGC	8 cut(s)	116, 197, 261, 273, 507, 1071, 1172, 1475
HinP1I	GCGC	8 cut(s)	116, 197, 261, 273, 507, 1071, 1172, 1475
HinfI	GANTC	8 cut(s)	83, 202, 255, 403, 849, 886, 1181, 1712
HpaII	CCGG	8 cut(s)	131, 136, 160, 276, 294, 747, 1191, 1794
HphI	GGTGA	6 cut(s)	283, 461, 532, 745, 940, 1207
Hpy166II	GTNNAC	2 cut(s)	1647, 1666
Hpy188I	TCNGA	3 cut(s)	254, 1536, 1717
Hpy188III	TCNNGA	4 cut(s)	91, 154, 400, 1501
Hpy8I	GTNNAC	2 cut(s)	1647, 1666
Hpy99I	CGWCG	4 cut(s)	332, 347, 1213, 1265
HpyAV	CCTTC	7 cut(s)	54, 187, 472, 1255, 1286, 1399, 1753
HpyCH4III	ACNGT	3 cut(s)	940, 1213, 1651
HpyCH4IV	ACGT	6 cut(s)	53, 108, 342, 843, 1263, 1464
HpyF3I	CTNAG	5 cut(s)	434, 668, 820, 1050, 1362
HpySE526I	ACGT	6 cut(s)	53, 108, 342, 843, 1263, 1464
Hsp92I	GRCGYC	4 cut(s)	128, 273, 354, 1353
Hsp92II	CATG	8 cut(s)	116, 719, 871, 1156, 1167, 1222, 1321, 1566
HspAI	GCGC	8 cut(s)	116, 197, 261, 273, 507, 1071, 1172, 1475
KasI	GGCGCC	1 cut(s)	272
KflI	GGGWCCC	1 cut(s)	1789
KpnI	GGTACC	1 cut(s)	1129
KroI	GCCGGC	1 cut(s)	746
KroNI	GCCGGC	1 cut(s)	748
Ksp22I	TGATCA	1 cut(s)	1653
KspI	CCGCGG	1 cut(s)	805
Kzo9I	GATC	4 cut(s)	145, 1531, 1615, 1653
LguI	GCTCTTC	1 cut(s)	688
LmnI	GCTCC	4 cut(s)	178, 442, 1012, 1437
LweI	GCATC	3 cut(s)	588, 657, 844
MaeI	CTAG	1 cut(s)	446
MaeII	ACGT	6 cut(s)	53, 108, 342, 843, 1263, 1464
MaeIII	GTNAC	6 cut(s)	449, 961, 1213, 1354, 1468, 1509
MalI	GATC	4 cut(s)	147, 1533, 1617, 1655
MboI	GATC	4 cut(s)	145, 1531, 1615, 1653
MfeI	CAATTG	3 cut(s)	906, 1416, 1581
MflI	RGATCY	1 cut(s)	1531
MhlI	GDGCHC	4 cut(s)	291, 323, 422, 683
MlsI	TGGCCA	1 cut(s)	1271
MluCI	AATT	7 cut(s)	103, 720, 906, 1404, 1416, 1581, 1840
MluI	ACGCGT	1 cut(s)	1689
MluNI	TGGCCA	1 cut(s)	1271
Mly113I	GGCGCC	1 cut(s)	273
MlyI	GAGTC	4 cut(s)	92, 211, 249, 1706
MmeI	TCCRAC	2 cut(s)	826, 1093
Mox20I	TGGCCA	1 cut(s)	1271
MroNI	GCCGGC	1 cut(s)	746
MroXI	GAANNNNTTC	1 cut(s)	1379
MscI	TGGCCA	1 cut(s)	1271
MseI	TTAA	1 cut(s)	1843
Msp20I	TGGCCA	1 cut(s)	1271
MspA1I	CMGCKG	3 cut(s)	804, 824, 1067
MspI	CCGG	8 cut(s)	131, 136, 160, 276, 294, 747, 1191, 1794
MspR9I	CCNGG	4 cut(s)	132, 160, 526, 1794
MunI	CAATTG	3 cut(s)	906, 1416, 1581
MvaI	CCWGG	1 cut(s)	526
MvnI	CGCG	5 cut(s)	199, 317, 804, 1475, 1691
NaeI	GCCGGC	1 cut(s)	748
NarI	GGCGCC	1 cut(s)	273
NciI	CCSGG	3 cut(s)	132, 160, 1794
NcoI	CCATGG	4 cut(s)	867, 1163, 1317, 1562
NdeII	GATC	4 cut(s)	145, 1531, 1615, 1653
NgoMIV	GCCGGC	1 cut(s)	746
NlaIII	CATG	8 cut(s)	116, 719, 871, 1156, 1167, 1222, 1321, 1566
NmeAIII	GCCGAG	1 cut(s)	237
NmuCI	GTSAC	4 cut(s)	449, 961, 1213, 1354
NsbI	TGCGCA	1 cut(s)	1173
PaeR7I	CTCGAG	1 cut(s)	488
PciSI	GCTCTTC	1 cut(s)	688
PcsI	WCGNNNNNNNCGW	5 cut(s)	351, 588, 849, 1611, 1626
PdiI	GCCGGC	1 cut(s)	748
PdmI	GAANNNNTTC	1 cut(s)	1379
PfeI	GAWTC	4 cut(s)	403, 849, 886, 1181
Pfl23II	CGTACG	1 cut(s)	339
PflFI	GACNNNGTC	1 cut(s)	160
PfoI	TCCNGGA	1 cut(s)	524
PleI	GAGTC	4 cut(s)	91, 210, 249, 1706
PluTI	GGCGCC	1 cut(s)	276
PpsI	GAGTC	4 cut(s)	91, 210, 249, 1706
Ppu21I	YACGTR	2 cut(s)	54, 1465
PpuMI	RGGWCCY	3 cut(s)	151, 238, 1789
Psp5II	RGGWCCY	3 cut(s)	151, 238, 1789
Psp6I	CCWGG	1 cut(s)	524
PspEI	GGTNACC	2 cut(s)	1213, 1468
PspGI	CCWGG	1 cut(s)	524
PspLI	CGTACG	1 cut(s)	339
PspOMI	GGGCCC	1 cut(s)	319
PspPI	GGNCC	8 cut(s)	133, 151, 238, 319, 320, 528, 797, 1789
PspPPI	RGGWCCY	3 cut(s)	151, 238, 1789
PspXI	VCTCGAGB	1 cut(s)	488
PstI	CTGCAG	1 cut(s)	1774
PsuI	RGATCY	1 cut(s)	1531
PsyI	GACNNNGTC	1 cut(s)	160
PvuII	CAGCTG	1 cut(s)	824
RsaI	GTAC	6 cut(s)	56, 341, 828, 1055, 1127, 1495
RsaNI	GTAC	6 cut(s)	55, 340, 827, 1054, 1126, 1494
SacII	CCGCGG	1 cut(s)	805
SapI	GCTCTTC	1 cut(s)	688
SaqAI	TTAA	1 cut(s)	1843
Sau3AI	GATC	4 cut(s)	145, 1531, 1615, 1653
Sau96I	GGNCC	8 cut(s)	133, 151, 238, 319, 320, 528, 797, 1789
SchI	GAGTC	4 cut(s)	92, 211, 249, 1706
ScrFI	CCNGG	4 cut(s)	132, 160, 526, 1794
SduI	GDGCHC	4 cut(s)	291, 323, 422, 683
SfaNI	GCATC	3 cut(s)	588, 657, 844
SfcI	CTRYAG	2 cut(s)	607, 1770
SfoI	GGCGCC	1 cut(s)	274
Sfr274I	CTCGAG	1 cut(s)	488
Sfr303I	CCGCGG	1 cut(s)	805
SgrBI	CCGCGG	1 cut(s)	805
SinI	GGWCC	4 cut(s)	151, 238, 528, 1789
SlaI	CTCGAG	1 cut(s)	488
SmlI	CTYRAG	2 cut(s)	488, 1252
SmoI	CTYRAG	2 cut(s)	488, 1252
Sse9I	AATT	7 cut(s)	103, 720, 906, 1404, 1416, 1581, 1840
SspDI	GGCGCC	1 cut(s)	272
SspI	AATATT	1 cut(s)	282
SspMI	CTAG	1 cut(s)	446
StyD4I	CCNGG	4 cut(s)	130, 158, 524, 1792
StyI	CCWWGG	4 cut(s)	867, 1163, 1317, 1562
TaaI	ACNGT	3 cut(s)	940, 1213, 1651
TaiI	ACGT	6 cut(s)	56, 111, 345, 846, 1266, 1467
TaqI	TCGA	8 cut(s)	399, 489, 618, 707, 780, 852, 1260, 1614
TasI	AATT	7 cut(s)	103, 720, 906, 1404, 1416, 1581, 1840
TatI	WGTACW	2 cut(s)	826, 1053
TauI	GCSGC	7 cut(s)	15, 507, 1162, 1338, 1353, 1480, 1740
TfiI	GAWTC	4 cut(s)	403, 849, 886, 1181
Tru1I	TTAA	1 cut(s)	1843
Tru9I	TTAA	1 cut(s)	1843
TscAI	CASTG	4 cut(s)	945, 966, 1560, 1810
TseFI	GTSAC	4 cut(s)	449, 961, 1213, 1354
Tsp45I	GTSAC	4 cut(s)	449, 961, 1213, 1354
TspDTI	ATGAA	7 cut(s)	206, 230, 454, 724, 1288, 1372, 1387
TspGWI	ACGGA	1 cut(s)	191
TspRI	CASTG	4 cut(s)	945, 966, 1560, 1810
Tth111I	GACNNNGTC	1 cut(s)	160
VpaK11BI	GGWCC	4 cut(s)	151, 238, 528, 1789
XagI	CCTNNNNNAGG	2 cut(s)	411, 433
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1560
XhoI	CTCGAG	1 cut(s)	488
XmnI	GAANNNNTTC	1 cut(s)	1379
XspI	CTAG	1 cut(s)	446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.