Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0002369

Physical Location & Seq

Genomic Coordinates Forward (+)

10854274 .. 10858124

Gene Structure 3851 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG119900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1839 bp

ATGGGGATGAGCGGAACGGAACCGAGCGGCGGCGGCGGCGGCGAGGGGCTGAAGAGGAGGGGGTGTTCGTGCGGAAAGAACGATTTCCTCCCGGAGGAGTCTTTCGAGAGCTGGGGCAATTACGCCAATGCGCTCCGGTCGACTCCGGAGAGGTTCAAGGACCGGTTCTTCACGCGGTCAGCCGACAGCACCGAGCTGGTGGAGGTGAAGGCGCGGAGCCAGCACGAGATGAAGAAGACGCTCAACTGGTGGGACCTCATCTGGTTCGGGCTCGGAGCCGTCATCGGCGCCGGAATCTTCGTCCTCACTGGACTCGAGGCCAAGAAACACGCCGGACCCGCCGTCGTCATGTCCTACGTCGTCTCCGGCGTCTCCGCCTTGCTCTCTGTTTTCTGTTACACAGAGTTCGCCGTCGAGATTCCCGTTGCAGGCGGCTCGTTTGCCTACCTAAGAGTGGAGCTTGGGGACTTCGTGGCCTACATTGCAGCAGGCAACATCCTTCTCGAATATGTCATAGGCGGGGCGGCAGTTGCGCGTTCGTGGACCTCCTACTTCACCTCCCTATGCAACCAGGACGATTCCAATGCTTTCCGAATCACTGTCCACGGTTGGTCGGAGGACTACAAGTACCTGGACCCCATCGCCATTGGGGTTATCATCTGTATCTGCATACTCGCCGTCTTGAGCACAAAGGGCTCCTCGCGCCTCAACTACGTCGCCTCCATAGTCCACGTCATCGTCATTCTCTTCATCATCATAGCAGGTCTCACCCAAGCAGATACTAACAACTACAAGGATTTTGCCCCAAATGGTGCTCGCGGCGTGTTCCAAGCCTCGGCAGTTCTCTTCTTCGCATACGTTGGATTTGACGCCGTTGCAACCATGGCCGAAGAGACCAAGAATCCTGCTCGTGACATCCCAATAGGGCTGGTTGGGTCCATGGTGATCACCACCACTCTTTATTGCTTGCTAGCCGTGACACTCTGCCTCATGGTACCGTATCAGGTAATTGATGAGGACGCTCCATTTTCAAAAGCGTTTGAGACAGTTGGTATGGGTTGGGCCAAGTATGTGGTTGCAGCTGGAGCTCTAAAAGGCATGACTAGTGTTTTGCTCGTCGGGGCAGTGGGTCAGGCCCGGTATTTGACCCACATTGCCCGAACCCACATGATGCCCCCATGGTTTGCTCAAGTTGATGCCAAGACAGGTACTCCAGTCAATGCCACAATTGTCATGCTCGCAGCCACAGCTGTAATCGCTTTCTTCACCAGCCTCGACATTCTTGCTAACCTTCTCTCCATTTCTACGCTATTCATCTTCAGCCTCGTTGCCATTGCTCTCCTCGTACGCCGTTACTATGTAACCGGTGTGACCACGGATGCAAACAGGAATAAGCTCATACTGTGCCTTGTTATGATACTCGCGGGTGCAATTGCCACTTCTGCTTACTGGGGTGTCAGCCATGATTGGGTAGGGTATTGTGTGACGGCTCCTATTTGGTTCTTGGGGACTTTGGGGATTTGGTTTCTTGTTCCGCAAGCAAGGAGTCCAAAGCTTTGGGGGGTGCCATTGGTTCCATGGTTGCCTTCACTTTCGATTGGCATCAACATTTTCTTGCTTGGTTCGATTGATGGTGATTCTTTTGTCAGGTTTGCAGTATGGACTGTGATTATCTTGGTTTACTACATTTTCTTTGGGTTGCATGCTTCATATGACACAGCAAAGGACTCTGAAGCTGCGAGGCTCGAGGGTGGGAAATACGAGACGAAGGTTGAAGGAACTAAGGACTCTGGTTTGGACGGTGCGTATAGCAAGGCCTCTACAGAACCTGCTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AA_permease_2 AA_permease_C

Protein Analysis

612

Amino Acids

66.06

Weight (kDa)

5.96

Isoelectric Point (pI)

32.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease_2	PF13520	81 - 452	9e-52	Amino acid permease
AA_permease	PF00324	86 - 455	2.3e-35	Amino acid permease
AA_permease_C	PF13906	520 - 570	1.1e-17	C-terminus of AA_permease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002369)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21120 AT4G21120
fragaria_vesca	FvH4_3g09930 FvH4_3g09940
malus_domestica	MD05G1274600.v1.1 MD05G1274700.v1.1 MD05G1274900.v1.1 MD10G1254300.v1.1
prunus_persica	Prupe.4G089000_v2.0.a1 Prupe.4G089100_v2.0.a1
pyrus_communis	pycom05g25610 pycom05g25630 pycom10g21000
rosa_chinensis	RchiOBHm_Chr5g0015621 RchiOBHm_Chr5g0015661 RchiOBHm_Chr5g0015691 RchiOBHm_Chr5g0015791
rosa_laevigata	RLG00000032214 RLG00000032217 RLG00000032219
rosa_multiflora	Rmu_sc0000843.1_g000004 Rmu_sc0000843.1_g000008 Rmu_sc0003230.1_g000003 Rmu_sc0003230.1_g000005
rosa_roxburghii	Rroxscaffold_1G00060830
rosa_rugosa	Rorug01G0078300 Rorug05G0029000 Rorug05G0029100
rosa_samantha	Rh5AG122300 Rh5AG122400 Rh5AG122600 Rh5BG120900 Rh5BG121200 Rh5BG121700 Rh5CG131300 Rh5CG131900 Rh5CG132800 Rh5DG119900 Rh5DG120600
rosa_wichuraiana	Rw5G010550 Rw5G010560 Rw5G010570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	752
Acc65I	GGTACC	1 cut(s)	994
AccB1I	GGYRCC	3 cut(s)	287, 994, 1564
AccBSI	CCGCTC	2 cut(s)	12, 27
AccI	GTMKAC	1 cut(s)	140
AccII	CGCG	6 cut(s)	175, 214, 535, 703, 819, 1424
AccIII	TCCGGA	1 cut(s)	145
AcoI	YGGCCR	1 cut(s)	885
AcuI	CTGAAG	3 cut(s)	71, 1303, 1752
AcyI	GRCGYC	3 cut(s)	288, 369, 870
AfaI	GTAC	4 cut(s)	629, 996, 1210, 1347
AfiI	CCNNNNNNNGG	6 cut(s)	29, 94, 428, 454, 835, 1468
AgeI	ACCGGT	2 cut(s)	162, 1364
AgsI	TTSAA	3 cut(s)	157, 1032, 1775
AhdI	GACNNNNNGTC	1 cut(s)	341
AhlI	ACTAGT	1 cut(s)	1103
AjiI	CACGTC	1 cut(s)	733
AjnI	CCWGG	2 cut(s)	570, 630
AloI	GAACNNNNNNTCC	2 cut(s)	49, 81
AluBI	AGCT	9 cut(s)	111, 196, 460, 1082, 1088, 1250, 1396, 1555, 1736
AluI	AGCT	9 cut(s)	111, 196, 460, 1082, 1088, 1250, 1396, 1555, 1736
Alw21I	GWGCWC	3 cut(s)	689, 817, 1090
Alw26I	GTCTC	6 cut(s)	367, 376, 770, 887, 1037, 1757
AlwNI	CAGNNNCTG	1 cut(s)	1829
Ama87I	CYCGRG	2 cut(s)	314, 1745
Aor13HI	TCCGGA	1 cut(s)	145
AoxI	GGCC	6 cut(s)	318, 474, 885, 1062, 1134, 1815
ApeKI	GCWGC	4 cut(s)	485, 1079, 1241, 1736
ArsI	GACNNNNNNTTYG	2 cut(s)	1093, 1125
AsiGI	ACCGGT	2 cut(s)	162, 1364
Asp700I	GAANNNNTTC	1 cut(s)	83
Asp718I	GGTACC	1 cut(s)	994
AspLEI	GCGC	5 cut(s)	133, 214, 290, 535, 705
AspS9I	GGNCC	8 cut(s)	160, 253, 335, 543, 634, 936, 1062, 1135
AsuC2I	CCSGG	2 cut(s)	92, 1138
AsuHPI	GGTGA	7 cut(s)	217, 547, 760, 940, 955, 1258, 1646
AsuNHI	GCTAGC	1 cut(s)	970
AvaI	CYCGRG	2 cut(s)	314, 1745
AvaII	GGWCC	6 cut(s)	160, 253, 335, 543, 634, 936
BaeI	ACNNNNGTAYC	2 cut(s)	1200, 1233
BanI	GGYRCC	3 cut(s)	287, 994, 1564
BanII	GRGCYC	3 cut(s)	273, 698, 1090
BauI	CACGAG	2 cut(s)	224, 909
BbsI	GAAGAC	1 cut(s)	242
Bbv12I	GWGCWC	3 cut(s)	689, 817, 1090
BbvI	GCAGC	4 cut(s)	497, 1091, 1253, 1723
BccI	CCATC	2 cut(s)	647, 1625
BceAI	ACGGC	8 cut(s)	263, 326, 395, 662, 857, 959, 1335, 1503
BcgI	CGANNNNNNTGC	6 cut(s)	417, 451, 566, 600, 1265, 1299
BciT130I	CCWGG	2 cut(s)	572, 632
BclI	TGATCA	1 cut(s)	945
BcnI	CCSGG	2 cut(s)	92, 1138
BcoDI	GTCTC	6 cut(s)	367, 376, 770, 887, 1037, 1757
BcuI	ACTAGT	1 cut(s)	1103
BfaI	CTAG	3 cut(s)	971, 1104, 1833
BfmI	CTRYAG	1 cut(s)	1821
BfoI	RGCGCY	1 cut(s)	291
BfuAI	ACCTGC	1 cut(s)	752
Bme1390I	CCNGG	4 cut(s)	92, 572, 632, 1138
Bme18I	GGWCC	6 cut(s)	160, 253, 335, 543, 634, 936
BmeRI	GACNNNNNGTC	1 cut(s)	341
BmeT110I	CYCGRG	2 cut(s)	314, 1745
BmgBI	CACGTC	1 cut(s)	733
BmgT120I	GGNCC	8 cut(s)	160, 253, 335, 543, 634, 936, 1062, 1135
BmrFI	CCNGG	4 cut(s)	92, 572, 632, 1138
BmrI	ACTGGG	1 cut(s)	1459
BmsI	GCATC	4 cut(s)	1161, 1186, 1369, 1611
BmtI	GCTAGC	1 cut(s)	974
BmuI	ACTGGG	1 cut(s)	1459
BpiI	GAAGAC	1 cut(s)	242
BpmI	CTGGAG	2 cut(s)	1104, 1197
BpuEI	CTTGAG	2 cut(s)	703, 1173
BpuMI	CCSGG	2 cut(s)	92, 1138
BsaBI	GATNNNNATC	1 cut(s)	1601
BsaHI	GRCGYC	3 cut(s)	288, 369, 870
BsaI	GGTCTC	2 cut(s)	770, 887
BsaJI	CCNNGG	7 cut(s)	604, 834, 882, 939, 1178, 1374, 1577
BsaWI	WCCGGW	4 cut(s)	135, 145, 162, 1364
Bsc4I	CCNNNNNNNGG	6 cut(s)	29, 94, 428, 454, 835, 1468
Bse118I	RCCGGY	2 cut(s)	162, 1364
Bse1I	ACTGG	4 cut(s)	251, 313, 1214, 1454
Bse3DI	GCAATG	3 cut(s)	480, 1152, 1332
Bse8I	GATNNNNATC	1 cut(s)	1601
BseAI	TCCGGA	1 cut(s)	145
BseBI	CCWGG	2 cut(s)	572, 632
BseDI	CCNNGG	7 cut(s)	604, 834, 882, 939, 1178, 1374, 1577
BseGI	GGATG	4 cut(s)	12, 495, 915, 1384
BseJI	GATNNNNATC	1 cut(s)	1601
BseLI	CCNNNNNNNGG	6 cut(s)	29, 94, 428, 454, 835, 1468
BseMI	GCAATG	3 cut(s)	480, 1152, 1332
BseNI	ACTGG	4 cut(s)	251, 313, 1214, 1454
BseRI	GAGGAG	4 cut(s)	70, 110, 688, 1331
BseXI	GCAGC	4 cut(s)	497, 1091, 1253, 1723
BseYI	CCCAGC	1 cut(s)	111
Bsh1236I	CGCG	6 cut(s)	175, 214, 535, 703, 819, 1424
Bsh1285I	CGRYCG	1 cut(s)	140
BshFI	GGCC	6 cut(s)	320, 476, 887, 1064, 1136, 1817
BshNI	GGYRCC	3 cut(s)	287, 994, 1564
BshTI	ACCGGT	2 cut(s)	162, 1364
BsiEI	CGRYCG	1 cut(s)	140
BsiHKAI	GWGCWC	3 cut(s)	689, 817, 1090
BsiHKCI	CYCGRG	2 cut(s)	314, 1745
BsiSI	CCGG	9 cut(s)	92, 136, 146, 163, 291, 333, 366, 1138, 1365
BsiWI	CGTACG	1 cut(s)	1345
BslFI	GGGAC	3 cut(s)	266, 479, 1522
BslI	CCNNNNNNNGG	6 cut(s)	29, 94, 428, 454, 835, 1468
BsmAI	GTCTC	6 cut(s)	367, 376, 770, 887, 1037, 1757
BsmBI	CGTCTC	3 cut(s)	367, 376, 1757
BsmFI	GGGAC	3 cut(s)	266, 479, 1522
BsnI	GGCC	6 cut(s)	320, 476, 887, 1064, 1136, 1817
Bso31I	GGTCTC	2 cut(s)	770, 887
BsoBI	CYCGRG	2 cut(s)	314, 1745
Bsp1286I	GDGCHC	5 cut(s)	273, 689, 698, 817, 1090
Bsp13I	TCCGGA	1 cut(s)	145
Bsp143I	GATC	1 cut(s)	945
Bsp19I	CCATGG	4 cut(s)	882, 939, 1178, 1577
BspANI	GGCC	6 cut(s)	320, 476, 887, 1064, 1136, 1817
BspEI	TCCGGA	1 cut(s)	145
BspFNI	CGCG	6 cut(s)	175, 214, 535, 703, 819, 1424
BspMI	ACCTGC	1 cut(s)	752
BspOI	GCTAGC	1 cut(s)	974
BspT107I	GGYRCC	3 cut(s)	287, 994, 1564
BspTNI	GGTCTC	2 cut(s)	770, 887
BsrBI	CCGCTC	2 cut(s)	12, 27
BsrDI	GCAATG	3 cut(s)	480, 1152, 1332
BsrFI	RCCGGY	2 cut(s)	162, 1364
BsrI	ACTGG	4 cut(s)	251, 313, 1214, 1454
BssAI	RCCGGY	2 cut(s)	162, 1364
BssECI	CCNNGG	7 cut(s)	604, 834, 882, 939, 1178, 1374, 1577
BssMI	GATC	1 cut(s)	945
BssNI	GRCGYC	3 cut(s)	288, 369, 870
BssSI	CACGAG	2 cut(s)	224, 909
BssT1I	CCWWGG	4 cut(s)	882, 939, 1178, 1577
Bst2BI	CACGAG	2 cut(s)	224, 909
Bst2UI	CCWGG	2 cut(s)	572, 632
Bst4CI	ACNGT	7 cut(s)	601, 608, 999, 1048, 1404, 1666, 1802
Bst6I	CTCTTC	4 cut(s)	47, 752, 851, 885
BstACI	GRCGYC	3 cut(s)	288, 369, 870
BstC8I	GCNNGC	9 cut(s)	221, 430, 490, 817, 968, 972, 1239, 1539, 1704
BstDEI	CTNAG	2 cut(s)	449, 1782
BstDSI	CCRYGG	6 cut(s)	604, 882, 939, 1178, 1374, 1577
BstENI	CCTNNNNNAGG	1 cut(s)	92
BstF5I	GGATG	4 cut(s)	12, 495, 915, 1384
BstFNI	CGCG	6 cut(s)	175, 214, 535, 703, 819, 1424
BstH2I	RGCGCY	1 cut(s)	291
BstHHI	GCGC	5 cut(s)	133, 214, 290, 535, 705
BstKTI	GATC	1 cut(s)	948
BstMAI	GTCTC	6 cut(s)	367, 376, 770, 887, 1037, 1757
BstMBI	GATC	1 cut(s)	945
BstMCI	CGRYCG	1 cut(s)	140
BstNI	CCWGG	2 cut(s)	572, 632
BstNSI	RCATGY	1 cut(s)	1706
BstSCI	CCNGG	4 cut(s)	90, 570, 630, 1136
BstSFI	CTRYAG	1 cut(s)	1821
BstUI	CGCG	6 cut(s)	175, 214, 535, 703, 819, 1424
BstV1I	GCAGC	4 cut(s)	497, 1091, 1253, 1723
BstV2I	GAAGAC	1 cut(s)	242
BstXI	CCANNNNNNTGG	2 cut(s)	1072, 1557
BsuRI	GGCC	6 cut(s)	320, 476, 887, 1064, 1136, 1817
BtgI	CCRYGG	6 cut(s)	604, 882, 939, 1178, 1374, 1577
BtgZI	GCGATG	1 cut(s)	625
BtrI	CACGTC	1 cut(s)	733
BtsCI	GGATG	4 cut(s)	12, 495, 915, 1384
BtsI	GCAGTG	1 cut(s)	1131
BtsIMutI	CAGTG	3 cut(s)	306, 597, 1131
BveI	ACCTGC	1 cut(s)	752
Cac8I	GCNNGC	9 cut(s)	221, 430, 490, 817, 968, 972, 1239, 1539, 1704
CaiI	CAGNNNCTG	1 cut(s)	1829
CfoI	GCGC	5 cut(s)	133, 214, 290, 535, 705
Cfr10I	RCCGGY	2 cut(s)	162, 1364
Cfr13I	GGNCC	8 cut(s)	160, 253, 335, 543, 634, 936, 1062, 1135
CseI	GACGC	4 cut(s)	247, 358, 878, 1028
Csp6I	GTAC	4 cut(s)	628, 995, 1209, 1346
CspAI	ACCGGT	2 cut(s)	162, 1364
CviQI	GTAC	4 cut(s)	628, 995, 1209, 1346
DdeI	CTNAG	2 cut(s)	449, 1782
DinI	GGCGCC	1 cut(s)	289
DpnI	GATC	1 cut(s)	947
DpnII	GATC	1 cut(s)	945
DriI	GACNNNNNGTC	1 cut(s)	341
EaeI	YGGCCR	1 cut(s)	885
Eam1104I	CTCTTC	4 cut(s)	47, 752, 851, 885
Eam1105I	GACNNNNNGTC	1 cut(s)	341
EarI	CTCTTC	4 cut(s)	47, 752, 851, 885
EciI	GGCGGA	1 cut(s)	364
Ecl136II	GAGCTC	1 cut(s)	1088
Eco130I	CCWWGG	4 cut(s)	882, 939, 1178, 1577
Eco147I	AGGCCT	1 cut(s)	1817
Eco24I	GRGCYC	3 cut(s)	273, 698, 1090
Eco31I	GGTCTC	2 cut(s)	770, 887
Eco47I	GGWCC	6 cut(s)	160, 253, 335, 543, 634, 936
Eco53kI	GAGCTC	1 cut(s)	1088
Eco57I	CTGAAG	3 cut(s)	71, 1303, 1752
Eco88I	CYCGRG	2 cut(s)	314, 1745
EcoICRI	GAGCTC	1 cut(s)	1088
EcoNI	CCTNNNNNAGG	1 cut(s)	92
EcoO109I	RGGNCCY	1 cut(s)	253
EcoRII	CCWGG	2 cut(s)	570, 630
EcoT14I	CCWWGG	4 cut(s)	882, 939, 1178, 1577
EcoT38I	GRGCYC	3 cut(s)	273, 698, 1090
EgeI	GGCGCC	1 cut(s)	289
EheI	GGCGCC	1 cut(s)	289
ErhI	CCWWGG	4 cut(s)	882, 939, 1178, 1577
Esp3I	CGTCTC	3 cut(s)	367, 376, 1757
FaqI	GGGAC	3 cut(s)	266, 479, 1522
FauI	CCCGC	3 cut(s)	346, 512, 1417
FauNDI	CATATG	1 cut(s)	1711
FbaI	TGATCA	1 cut(s)	945
FblI	GTMKAC	1 cut(s)	140
FokI	GGATG	4 cut(s)	19, 482, 902, 1391
FriOI	GRGCYC	3 cut(s)	273, 698, 1090
FspBI	CTAG	3 cut(s)	971, 1104, 1833
GlaI	GCGC	5 cut(s)	132, 213, 289, 534, 704
GsaI	CCCAGC	1 cut(s)	115
GsuI	CTGGAG	2 cut(s)	1104, 1197
HaeII	RGCGCY	1 cut(s)	291
HaeIII	GGCC	6 cut(s)	320, 476, 887, 1064, 1136, 1817
HapII	CCGG	9 cut(s)	92, 136, 146, 163, 291, 333, 366, 1138, 1365
HgaI	GACGC	4 cut(s)	247, 358, 878, 1028
HhaI	GCGC	5 cut(s)	133, 214, 290, 535, 705
Hin1I	GRCGYC	3 cut(s)	288, 369, 870
Hin6I	GCGC	5 cut(s)	131, 212, 288, 533, 703
HinP1I	GCGC	5 cut(s)	131, 212, 288, 533, 703
HincII	GTYRAC	1 cut(s)	141
HindII	GTYRAC	1 cut(s)	141
HindIII	AAGCTT	1 cut(s)	1553
HpaII	CCGG	9 cut(s)	92, 136, 146, 163, 291, 333, 366, 1138, 1365
HphI	GGTGA	7 cut(s)	217, 547, 760, 940, 955, 1258, 1646
Hpy166II	GTNNAC	5 cut(s)	141, 543, 604, 730, 1681
Hpy188I	TCNGA	4 cut(s)	275, 593, 616, 1732
Hpy188III	TCNNGA	6 cut(s)	106, 146, 415, 503, 682, 911
Hpy8I	GTNNAC	5 cut(s)	141, 543, 604, 730, 1681
Hpy99I	CGWCG	5 cut(s)	347, 362, 416, 719, 1121
HpyAV	CCTTC	6 cut(s)	202, 509, 1301, 1596, 1762, 1769
HpyCH4III	ACNGT	7 cut(s)	601, 608, 999, 1048, 1404, 1666, 1802
HpyCH4IV	ACGT	4 cut(s)	357, 714, 732, 858
HpyF3I	CTNAG	2 cut(s)	449, 1782
HpySE526I	ACGT	4 cut(s)	357, 714, 732, 858
Hsp92I	GRCGYC	3 cut(s)	288, 369, 870
HspAI	GCGC	5 cut(s)	131, 212, 288, 533, 703
KasI	GGCGCC	1 cut(s)	287
Kpn2I	TCCGGA	1 cut(s)	145
KpnI	GGTACC	1 cut(s)	998
Ksp22I	TGATCA	1 cut(s)	945
Kzo9I	GATC	1 cut(s)	945
LmnI	GCTCC	8 cut(s)	138, 216, 275, 457, 701, 1027, 1085, 1495
Lsp1109I	GCAGC	4 cut(s)	497, 1091, 1253, 1723
LweI	GCATC	4 cut(s)	1161, 1186, 1369, 1611
MaeI	CTAG	3 cut(s)	971, 1104, 1833
MaeII	ACGT	4 cut(s)	357, 714, 732, 858
MaeIII	GTNAC	7 cut(s)	395, 911, 976, 1352, 1360, 1369, 1483
MalI	GATC	1 cut(s)	947
MbiI	CCGCTC	2 cut(s)	12, 27
MboI	GATC	1 cut(s)	945
MfeI	CAATTG	2 cut(s)	1227, 1431
MhlI	GDGCHC	5 cut(s)	273, 689, 698, 817, 1090
MluCI	AATT	4 cut(s)	118, 1008, 1227, 1431
Mly113I	GGCGCC	1 cut(s)	288
MlyI	GAGTC	6 cut(s)	107, 136, 306, 1555, 1721, 1781
MmeI	TCCRAC	2 cut(s)	594, 841
MroI	TCCGGA	1 cut(s)	145
MroXI	GAANNNNTTC	1 cut(s)	83
MseI	TTAA	1 cut(s)	1837
MspA1I	CMGCKG	2 cut(s)	1082, 1250
MspI	CCGG	9 cut(s)	92, 136, 146, 163, 291, 333, 366, 1138, 1365
MspR9I	CCNGG	4 cut(s)	92, 572, 632, 1138
MunI	CAATTG	2 cut(s)	1227, 1431
MvaI	CCWGG	2 cut(s)	572, 632
MvnI	CGCG	6 cut(s)	175, 214, 535, 703, 819, 1424
NarI	GGCGCC	1 cut(s)	288
NciI	CCSGG	2 cut(s)	92, 1138
NcoI	CCATGG	4 cut(s)	882, 939, 1178, 1577
NdeI	CATATG	1 cut(s)	1711
NdeII	GATC	1 cut(s)	945
NheI	GCTAGC	1 cut(s)	970
NmeAIII	GCCGAG	1 cut(s)	815
NmuCI	GTSAC	4 cut(s)	911, 976, 1369, 1483
NspI	RCATGY	1 cut(s)	1706
PaeI	GCATGC	1 cut(s)	1706
PaeR7I	CTCGAG	2 cut(s)	314, 1745
PceI	AGGCCT	1 cut(s)	1817
PcsI	WCGNNNNNNNCGW	2 cut(s)	366, 420
PdmI	GAANNNNTTC	1 cut(s)	83
PfeI	GAWTC	6 cut(s)	294, 418, 578, 594, 901, 1637
Pfl23II	CGTACG	1 cut(s)	1345
PfoI	TCCNGGA	1 cut(s)	90
PinAI	ACCGGT	2 cut(s)	162, 1364
PleI	GAGTC	6 cut(s)	106, 136, 306, 1554, 1721, 1781
PluTI	GGCGCC	1 cut(s)	291
PpsI	GAGTC	6 cut(s)	106, 136, 306, 1554, 1721, 1781
PpuMI	RGGWCCY	1 cut(s)	253
Psp124BI	GAGCTC	1 cut(s)	1090
Psp5II	RGGWCCY	1 cut(s)	253
Psp6I	CCWGG	2 cut(s)	570, 630
PspFI	CCCAGC	1 cut(s)	111
PspGI	CCWGG	2 cut(s)	570, 630
PspLI	CGTACG	1 cut(s)	1345
PspPI	GGNCC	8 cut(s)	160, 253, 335, 543, 634, 936, 1062, 1135
PspPPI	RGGWCCY	1 cut(s)	253
PspXI	VCTCGAGB	2 cut(s)	314, 1745
PstNI	CAGNNNCTG	1 cut(s)	1829
PvuII	CAGCTG	2 cut(s)	1082, 1250
RsaI	GTAC	4 cut(s)	629, 996, 1210, 1347
RsaNI	GTAC	4 cut(s)	628, 995, 1209, 1346
SacI	GAGCTC	1 cut(s)	1090
SalI	GTCGAC	1 cut(s)	139
SaqAI	TTAA	1 cut(s)	1837
Sau3AI	GATC	1 cut(s)	945
Sau96I	GGNCC	8 cut(s)	160, 253, 335, 543, 634, 936, 1062, 1135
SchI	GAGTC	6 cut(s)	107, 136, 306, 1555, 1721, 1781
ScrFI	CCNGG	4 cut(s)	92, 572, 632, 1138
SduI	GDGCHC	5 cut(s)	273, 689, 698, 817, 1090
SfaNI	GCATC	4 cut(s)	1161, 1186, 1369, 1611
SfcI	CTRYAG	1 cut(s)	1821
SfoI	GGCGCC	1 cut(s)	289
Sfr274I	CTCGAG	2 cut(s)	314, 1745
SinI	GGWCC	6 cut(s)	160, 253, 335, 543, 634, 936
SlaI	CTCGAG	2 cut(s)	314, 1745
SmlI	CTYRAG	4 cut(s)	314, 682, 1188, 1745
SmoI	CTYRAG	4 cut(s)	314, 682, 1188, 1745
SpeI	ACTAGT	1 cut(s)	1103
SphI	GCATGC	1 cut(s)	1706
Sse9I	AATT	4 cut(s)	118, 1008, 1227, 1431
SseBI	AGGCCT	1 cut(s)	1817
SspDI	GGCGCC	1 cut(s)	287
SspMI	CTAG	3 cut(s)	971, 1104, 1833
SstI	GAGCTC	1 cut(s)	1090
StuI	AGGCCT	1 cut(s)	1817
StyD4I	CCNGG	4 cut(s)	90, 570, 630, 1136
StyI	CCWWGG	4 cut(s)	882, 939, 1178, 1577
TaaI	ACNGT	7 cut(s)	601, 608, 999, 1048, 1404, 1666, 1802
TaiI	ACGT	4 cut(s)	360, 717, 735, 861
TaqI	TCGA	9 cut(s)	105, 140, 315, 414, 504, 1275, 1595, 1625, 1746
TasI	AATT	4 cut(s)	118, 1008, 1227, 1431
TauI	GCSGC	8 cut(s)	30, 33, 36, 39, 42, 435, 527, 822
TfiI	GAWTC	6 cut(s)	294, 418, 578, 594, 901, 1637
Tru1I	TTAA	1 cut(s)	1837
Tru9I	TTAA	1 cut(s)	1837
TscAI	CASTG	3 cut(s)	313, 604, 1131
TseFI	GTSAC	4 cut(s)	911, 976, 1369, 1483
TseI	GCWGC	4 cut(s)	485, 1079, 1241, 1736
Tsp45I	GTSAC	4 cut(s)	911, 976, 1369, 1483
TspDTI	ATGAA	4 cut(s)	245, 739, 1303, 1698
TspGWI	ACGGA	2 cut(s)	32, 1391
TspRI	CASTG	3 cut(s)	313, 604, 1131
VpaK11BI	GGWCC	6 cut(s)	160, 253, 335, 543, 634, 936
XagI	CCTNNNNNAGG	1 cut(s)	92
XceI	RCATGY	1 cut(s)	1706
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1575
XhoI	CTCGAG	2 cut(s)	314, 1745
XmiI	GTMKAC	1 cut(s)	140
XmnI	GAANNNNTTC	1 cut(s)	83
XspI	CTAG	3 cut(s)	971, 1104, 1833

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.