MD05G1323000.v1.1

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001643
Physical Location & Seq
Forward (+)
45028507 .. 45033663
5157 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1323000.v1.1.491

Sequence Viewer

Length: 1233 bp
ATGAAGTCACCAAACTCATGGATATGGATGTTCAAGAAAAAGAATTCTTTTTCTGATGGGGGTAAAAGCGGAATCAAGATGAAGCAGTTGTCATTTATGGGAGTTGTATGTACAGTAATGTTATTCGTTGTGTATAGAACCACCAACTATCAGTACCAAGCTTATCAGCAGACAGAGATGGACGCAAAGTGGAATCCCTTTGACACAGTGAAGGAAATTTTTGTGTCTTCGAGAAAGTTGGACAGTTTACCACGTGGCATTATACAACCTAGGTCAGATTTAGAGCAAAGGCCTCTATGGTTGACAAGTAGTTCAATGTTAAAGGTCGGTGATGACAGCAACCGTAACTTGCTGGCTATTCCGGTTGGTATTAAGCAAAAGGATAGTGTTGATGCTATTGTGGGAAAGTTTCTTCCAGAAAATTTTACCGTTATCCTCTTCCATTATGATGGGAATGTTGATGGGTGGTGGGATCTTGAGTGGAGTAACGAGGCTGTACACATAGTTGCCCAAAACCAAACAAAGTGGTGGTTTGCCAAACGCTTTCTACATCCAGATATTGTGTCCATTTATGATTACATATTCCTTTGGGATGAGGATTTGGGGGTTGAGAATTTTCATCCAGGAAGATACCTTGAAATTGTAAAAGAAGCGGGACTGGAGATATCTCAGCCAGCTTTGCATCCAAATTCAACAGATATACATCATAGAATTACGATTCGTGCTAAAAAAAAGAAGTTTCATAGAAGAGTTTATGACACCAGAGGCAGCACAAAGTGTACAGAAGCAAGTCAGGGACCACCATGCACCGGGTTTGTGGAAGGCATGGCTCCGGTTTTTTCAAGAGCTGCTTGGCATTGTACTTGGCATCTTATACAGAATGATCTTGTACATGGATGGGGTCTGGACATGAAACTTGGGTATTGTGCACAGGGAGACCGTACCAAGAAAGTGGGAGTGGTTGATAGCGAATATATTGTTCATAAGGGAATACAAACTTTGGGAGAAGGTGGTTCATCGACAGACAAGGTTCCTGAGAAGAAGAGTAAGAAACGAGCTGCTCCTGGGCATGACATGCGAATTGAGATTAGGAGGCAGTCTACACGGGAGTATACAACCTTCAAAGAGCGGTGGAATAACGCGGTAGAAGAGGACTTGAAGTGGATTGATCCATTTAAAAGCAGCCTAAAACGCAGGAAACAGCGTAAACGCAACAACCAGTTGAGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

411

Amino Acids

47.47

Weight (kDa)

9.43

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 78 - 383 9.7e-136 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1129
AccI GTMKAC 2 cut(s) 1100, 1112
AccII CGCG 1 cut(s) 1142
AciI CCGC 4 cut(s) 69, 653, 1129, 1142
AclWI GGATC 2 cut(s) 480, 1163
AcsI RAATTY 5 cut(s) 43, 216, 421, 613, 688
AcvI CACGTG 1 cut(s) 254
AdeI CACNNNGTG 1 cut(s) 777
AfaI GTAC 7 cut(s) 112, 155, 498, 781, 862, 891, 943
AfiI CCNNNNNNNGG 1 cut(s) 809
AgsI TTSAA 7 cut(s) 34, 315, 638, 693, 843, 1123, 1159
AjnI CCWGG 2 cut(s) 622, 1063
AluBI AGCT 5 cut(s) 161, 677, 848, 1058, 1227
AluI AGCT 5 cut(s) 161, 677, 848, 1058, 1227
Alw21I GWGCWC 2 cut(s) 931, 1229
Alw26I GTCTC 1 cut(s) 930
Alw44I GTGCAC 1 cut(s) 927
AlwI GGATC 2 cut(s) 480, 1163
AoxI GGCC 1 cut(s) 290
ApaLI GTGCAC 1 cut(s) 927
ApeKI GCWGC 4 cut(s) 768, 848, 1058, 1182
ApoI RAATTY 5 cut(s) 43, 216, 421, 613, 688
AspA2I CCTAGG 1 cut(s) 269
AspS9I GGNCC 1 cut(s) 797
AsuC2I CCSGG 1 cut(s) 811
AsuHPI GGTGA 1 cut(s) 341
AvaII GGWCC 1 cut(s) 797
AvrII CCTAGG 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 931
BanII GRGCYC 1 cut(s) 1229
BarI GAAGNNNNNNTAC 2 cut(s) 1103, 1135
BbrPI CACGTG 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 2 cut(s) 931, 1229
BbvI GCAGC 4 cut(s) 780, 835, 1045, 1194
BccI CCATC 5 cut(s) 50, 172, 443, 455, 891
BciT130I CCWGG 2 cut(s) 624, 1065
BcnI CCSGG 1 cut(s) 811
BcoDI GTCTC 1 cut(s) 930
BfaI CTAG 1 cut(s) 270
BisI GCNGC 4 cut(s) 769, 849, 1059, 1183
BlnI CCTAGG 1 cut(s) 269
BlsI GCNGC 4 cut(s) 770, 850, 1060, 1184
Bme1390I CCNGG 3 cut(s) 624, 811, 1065
Bme18I GGWCC 1 cut(s) 797
BmgT120I GGNCC 1 cut(s) 797
BmiI GGNNCC 3 cut(s) 798, 831, 1032
BmrFI CCNGG 3 cut(s) 624, 811, 1065
BmsI GCATC 3 cut(s) 382, 691, 877
BpiI GAAGAC 1 cut(s) 219
BpmI CTGGAG 1 cut(s) 680
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 811
BsaAI YACGTR 1 cut(s) 254
BsaBI GATNNNNATC 1 cut(s) 702
BsaI GGTCTC 1 cut(s) 930
BsaJI CCNNGG 2 cut(s) 269, 1064
BsaWI WCCGGW 2 cut(s) 361, 832
Bsc4I CCNNNNNNNGG 1 cut(s) 809
Bse1I ACTGG 2 cut(s) 663, 1219
Bse8I GATNNNNATC 1 cut(s) 702
BseBI CCWGG 2 cut(s) 624, 1065
BseDI CCNNGG 2 cut(s) 269, 1064
BseGI GGATG 6 cut(s) 33, 550, 598, 619, 682, 902
BseJI GATNNNNATC 1 cut(s) 702
BseLI CCNNNNNNNGG 1 cut(s) 809
BseMII CTCAG 2 cut(s) 683, 1026
BseNI ACTGG 2 cut(s) 663, 1219
BseSI GKGCMC 1 cut(s) 931
BseXI GCAGC 4 cut(s) 780, 835, 1045, 1194
Bsh1236I CGCG 1 cut(s) 1142
BshFI GGCC 1 cut(s) 292
BsiHKAI GWGCWC 2 cut(s) 931, 1229
BsiSI CCGG 3 cut(s) 362, 810, 833
BslFI GGGAC 2 cut(s) 669, 810
BslI CCNNNNNNNGG 1 cut(s) 809
BsmAI GTCTC 1 cut(s) 930
BsmFI GGGAC 2 cut(s) 669, 810
BsnI GGCC 1 cut(s) 292
Bso31I GGTCTC 1 cut(s) 930
Bsp1286I GDGCHC 2 cut(s) 931, 1229
Bsp1407I TGTACA 4 cut(s) 110, 496, 779, 889
Bsp143I GATC 3 cut(s) 472, 883, 1168
BspACI CCGC 4 cut(s) 69, 653, 1129, 1142
BspANI GGCC 1 cut(s) 292
BspCNI CTCAG 2 cut(s) 682, 1027
BspFNI CGCG 1 cut(s) 1142
BspLI GGNNCC 3 cut(s) 798, 831, 1032
BspPI GGATC 2 cut(s) 480, 1163
BspTNI GGTCTC 1 cut(s) 930
BsrBI CCGCTC 1 cut(s) 1129
BsrGI TGTACA 4 cut(s) 110, 496, 779, 889
BsrI ACTGG 2 cut(s) 663, 1219
BssECI CCNNGG 2 cut(s) 269, 1064
BssMI GATC 3 cut(s) 472, 883, 1168
BssNAI GTATAC 1 cut(s) 1113
BssT1I CCWWGG 1 cut(s) 269
Bst1107I GTATAC 1 cut(s) 1113
Bst2UI CCWGG 2 cut(s) 624, 1065
Bst4CI ACNGT 6 cut(s) 115, 208, 245, 344, 430, 941
Bst6I CTCTTC 4 cut(s) 443, 742, 1037, 1143
BstAPI GCANNNNNTGC 1 cut(s) 1075
BstAUI TGTACA 4 cut(s) 110, 496, 779, 889
BstBAI YACGTR 1 cut(s) 254
BstC8I GCNNGC 2 cut(s) 354, 675
BstDEI CTNAG 2 cut(s) 669, 1035
BstF5I GGATG 6 cut(s) 33, 550, 598, 619, 682, 902
BstFNI CGCG 1 cut(s) 1142
BstKTI GATC 3 cut(s) 475, 886, 1171
BstMAI GTCTC 1 cut(s) 930
BstMBI GATC 3 cut(s) 472, 883, 1168
BstMWI GCNNNNNNNGC 3 cut(s) 679, 1075, 1191
BstNI CCWGG 2 cut(s) 624, 1065
BstNSI RCATGY 1 cut(s) 1078
BstSCI CCNGG 3 cut(s) 622, 809, 1063
BstSLI GKGCMC 1 cut(s) 931
BstUI CGCG 1 cut(s) 1142
BstV1I GCAGC 4 cut(s) 780, 835, 1045, 1194
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 1 cut(s) 472
BstXI CCANNNNNNTGG 3 cut(s) 18, 449, 952
BstYI RGATCY 1 cut(s) 472
BstZ17I GTATAC 1 cut(s) 1113
BsuRI GGCC 1 cut(s) 292
BtsCI GGATG 6 cut(s) 33, 550, 598, 619, 682, 902
BtsIMutI CAGTG 1 cut(s) 213
Cac8I GCNNGC 2 cut(s) 354, 675
Cfr13I GGNCC 1 cut(s) 797
CseI GACGC 1 cut(s) 191
Csp6I GTAC 7 cut(s) 111, 154, 497, 780, 861, 890, 942
CspCI CAANNNNNGTGG 2 cut(s) 506, 541
CviAII CATG 7 cut(s) 18, 804, 826, 893, 910, 1070, 1075
CviQI GTAC 7 cut(s) 111, 154, 497, 780, 861, 890, 942
DdeI CTNAG 2 cut(s) 669, 1035
DpnI GATC 3 cut(s) 474, 885, 1170
DpnII GATC 3 cut(s) 472, 883, 1168
DraI TTTAAA 1 cut(s) 1177
DraIII CACNNNGTG 1 cut(s) 777
Eam1104I CTCTTC 4 cut(s) 443, 742, 1037, 1143
EarI CTCTTC 4 cut(s) 443, 742, 1037, 1143
Ecl136II GAGCTC 1 cut(s) 1227
Eco130I CCWWGG 1 cut(s) 269
Eco147I AGGCCT 1 cut(s) 292
Eco24I GRGCYC 1 cut(s) 1229
Eco31I GGTCTC 1 cut(s) 930
Eco32I GATATC 1 cut(s) 666
Eco47I GGWCC 1 cut(s) 797
Eco53kI GAGCTC 1 cut(s) 1227
Eco72I CACGTG 1 cut(s) 254
EcoICRI GAGCTC 1 cut(s) 1227
EcoRI GAATTC 1 cut(s) 43
EcoRII CCWGG 2 cut(s) 622, 1063
EcoRV GATATC 1 cut(s) 666
EcoT14I CCWWGG 1 cut(s) 269
EcoT38I GRGCYC 1 cut(s) 1229
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 7 cut(s) 21, 807, 829, 896, 913, 1073, 1078
FalI AAGNNNNNCTT 2 cut(s) 835, 867
FaqI GGGAC 2 cut(s) 669, 810
FatI CATG 7 cut(s) 17, 803, 825, 892, 909, 1069, 1074
FauI CCCGC 1 cut(s) 646
FblI GTMKAC 2 cut(s) 1100, 1112
Fnu4HI GCNGC 4 cut(s) 769, 849, 1059, 1183
FokI GGATG 6 cut(s) 40, 537, 605, 606, 669, 909
FriOI GRGCYC 1 cut(s) 1229
Fsp4HI GCNGC 4 cut(s) 769, 849, 1059, 1183
FspBI CTAG 1 cut(s) 270
GluI GCNGC 4 cut(s) 769, 849, 1059, 1183
GsuI CTGGAG 1 cut(s) 680
HaeIII GGCC 1 cut(s) 292
HapII CCGG 3 cut(s) 362, 810, 833
HgaI GACGC 1 cut(s) 191
Hin1II CATG 7 cut(s) 21, 807, 829, 896, 913, 1073, 1078
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 3 cut(s) 72, 193, 718
HpaII CCGG 3 cut(s) 362, 810, 833
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 8 cut(s) 248, 303, 499, 780, 929, 1101, 1113, 1208
Hpy188I TCNGA 2 cut(s) 55, 277
Hpy188III TCNNGA 9 cut(s) 34, 76, 231, 416, 476, 554, 843, 905, 1034
Hpy8I GTNNAC 8 cut(s) 248, 303, 499, 780, 929, 1101, 1113, 1208
HpyAV CCTTC 4 cut(s) 205, 815, 1001, 1129
HpyCH4III ACNGT 6 cut(s) 115, 208, 245, 344, 430, 941
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 3 cut(s) 682, 807, 929
HpyF10VI GCNNNNNNNGC 3 cut(s) 679, 1075, 1191
HpyF3I CTNAG 2 cut(s) 669, 1035
HpySE526I ACGT 1 cut(s) 253
Hsp92II CATG 7 cut(s) 21, 807, 829, 896, 913, 1073, 1078
Kzo9I GATC 3 cut(s) 472, 883, 1168
LmnI GCTCC 2 cut(s) 835, 1066
Lsp1109I GCAGC 4 cut(s) 780, 835, 1045, 1194
LweI GCATC 3 cut(s) 382, 691, 877
MaeI CTAG 1 cut(s) 270
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 3 cut(s) 6, 344, 485
MalI GATC 3 cut(s) 474, 885, 1170
MbiI CCGCTC 1 cut(s) 1129
MboI GATC 3 cut(s) 472, 883, 1168
MboII GAAGA 8 cut(s) 219, 404, 430, 639, 759, 1051, 1054, 1160
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 2 cut(s) 931, 1229
MluCI AATT 8 cut(s) 43, 216, 421, 613, 639, 688, 711, 1080
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 7 cut(s) 303, 446, 484, 589, 758, 1086, 1144
MseI TTAA 4 cut(s) 320, 372, 1176, 1231
MslI CAYNNNNRTG 2 cut(s) 22, 447
MspI CCGG 3 cut(s) 362, 810, 833
MspR9I CCNGG 3 cut(s) 624, 811, 1065
MvaI CCWGG 2 cut(s) 624, 1065
MvnI CGCG 1 cut(s) 1142
MwoI GCNNNNNNNGC 3 cut(s) 679, 1075, 1191
NciI CCSGG 1 cut(s) 811
NdeII GATC 3 cut(s) 472, 883, 1168
NlaIII CATG 7 cut(s) 21, 807, 829, 896, 913, 1073, 1078
NlaIV GGNNCC 3 cut(s) 798, 831, 1032
NmuCI GTSAC 1 cut(s) 6
NspI RCATGY 1 cut(s) 1078
PceI AGGCCT 1 cut(s) 292
PfeI GAWTC 3 cut(s) 72, 193, 718
PfoI TCCNGGA 1 cut(s) 622
PkrI GCNGC 4 cut(s) 770, 850, 1060, 1184
PmaCI CACGTG 1 cut(s) 254
PmlI CACGTG 1 cut(s) 254
Ppu21I YACGTR 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 1229
Psp6I CCWGG 2 cut(s) 622, 1063
PspCI CACGTG 1 cut(s) 254
PspGI CCWGG 2 cut(s) 622, 1063
PspN4I GGNNCC 3 cut(s) 798, 831, 1032
PspPI GGNCC 1 cut(s) 797
PsuI RGATCY 1 cut(s) 472
RsaI GTAC 7 cut(s) 112, 155, 498, 781, 862, 891, 943
RsaNI GTAC 7 cut(s) 111, 154, 497, 780, 861, 890, 942
RseI CAYNNNNRTG 2 cut(s) 22, 447
SacI GAGCTC 1 cut(s) 1229
SaqAI TTAA 4 cut(s) 320, 372, 1176, 1231
SatI GCNGC 4 cut(s) 769, 849, 1059, 1183
Sau3AI GATC 3 cut(s) 472, 883, 1168
Sau96I GGNCC 1 cut(s) 797
ScrFI CCNGG 3 cut(s) 624, 811, 1065
SduI GDGCHC 2 cut(s) 931, 1229
SfaNI GCATC 3 cut(s) 382, 691, 877
SinI GGWCC 1 cut(s) 797
SmiMI CAYNNNNRTG 2 cut(s) 22, 447
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 8 cut(s) 43, 216, 421, 613, 639, 688, 711, 1080
SseBI AGGCCT 1 cut(s) 292
SsiI CCGC 4 cut(s) 69, 653, 1129, 1142
SspMI CTAG 1 cut(s) 270
SstI GAGCTC 1 cut(s) 1229
StuI AGGCCT 1 cut(s) 292
StyD4I CCNGG 3 cut(s) 622, 809, 1063
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 6 cut(s) 115, 208, 245, 344, 430, 941
TaiI ACGT 1 cut(s) 256
TaqI TCGA 2 cut(s) 230, 1019
TasI AATT 8 cut(s) 43, 216, 421, 613, 639, 688, 711, 1080
TatI WGTACW 5 cut(s) 110, 496, 779, 860, 889
TfiI GAWTC 3 cut(s) 72, 193, 718
Tru1I TTAA 4 cut(s) 320, 372, 1176, 1231
Tru9I TTAA 4 cut(s) 320, 372, 1176, 1231
TscAI CASTG 1 cut(s) 213
TseFI GTSAC 1 cut(s) 6
TseI GCWGC 4 cut(s) 768, 848, 1058, 1182
Tsp45I GTSAC 1 cut(s) 6
TspDTI ATGAA 7 cut(s) 17, 95, 608, 731, 926, 971, 1005
TspRI CASTG 1 cut(s) 213
VneI GTGCAC 1 cut(s) 927
VpaK11BI GGWCC 1 cut(s) 797
XapI RAATTY 5 cut(s) 43, 216, 421, 613, 688
XceI RCATGY 1 cut(s) 1078
XmaJI CCTAGG 1 cut(s) 269
XmiI GTMKAC 2 cut(s) 1100, 1112
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.