MD10G1300800.v1.1

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001643
Physical Location & Seq
Forward (+)
38810974 .. 38816505
5532 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1300800.v1.1.491

Sequence Viewer

Length: 1230 bp
ATGAAGTCGCCAAACTCATGGATATGGATGTTCAAGAAAAAGAATTCTTTTTCTGATGGGGGGAAAAGCGGAATGAAGATGAAGCAGCTGTCATTTATGGGAGTTACATGTACCGTAATGTTATTCATAGTGTATAGAACCACCAACTTTCAGTATCAAGCTTATCAGCAGACGGAGATTGACGCAAAGTGGAATCCCTTTGACACAGTAAAGGAAATTTTTGCGACTTCGAGAAAGTTGGACAGTTTACCTCGTGGGATTATACAGCCTAGGTCAGATTTAGAGCAAAGGCCTCTATGGTTGACAAGTAGTTCAAGGTTAAAGGTCGATGATTATAGCAACCGTAACTTGCTGGCTATTCCGGTTGGTATTAAGCAAAAGGATAGTGTTGATGCTATTGTGAGAAAGTTTCTTCCAGAAAATTTTACCTTTATCCTATTCCATTATGATGGGAATGTTGATGGGTGGTGGGATCTTGAGTGGAGTAACGAGGCCATACACATAGTTGCCCAGAACCAAACAAAGTGGTGGTTTGCGAAACGCTTTCTGCATCCAGATATTGTGTCCATTTATGATTACGTATTCCTTTGGGATGAGGATTTGGGGGTTGATAATTTTCATCCAGGAAGATACCTTGAAATTGTGAAAGAAGCGGGACTGGAGATATCTCAGCCAGCTTTGCATCCAAATTCAACAGATATACATCATAGAATTACAATTCGTGCTAAAAATAAGAAGTTTCATAGAAGAGTTTATGACACCAGAGGCAGCACAAAGTGTACAGAAGCAAGTCAGGGACCACCATGTACCGGGTTTGTGGAAGGCATGGCTCCCGTTTTTTCAAGAGCTGCCTGGCATTGTACTTGGCATCTTATACAGAATGATCTTGTACATGGATGGGGTCTGGATATGAGACTTGGGTATTGTGCACAGGGAGACCGTACCAAGAAAGTGGGAGTTGTTGATAGTGAATATATTGTTCACAAGGGAATACAAACTTTGGGATATGGTGGTTCGTCGACAAACAAGGTTCCTGAGAAGAAGAGTAAGAAACGAGCTGCTTCAGGGCATGACGTGCGAATTGAGATTAGGAGGCAGTCAACGCGGGAGTTTATAACCTTCAAACAGCGGTGGAACAACGCAGTAGAAGAAGACCCGAGCTGGGCTGATCCATTTAAAAGTAGCCTAAAACGCAGGAAACAGCGTAAATTCGACAACTTGAGATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

410

Amino Acids

47.55

Weight (kDa)

9.55

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 78 - 383 2.1e-134 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1115
AccI GTMKAC 1 cut(s) 1019
AccII CGCG 1 cut(s) 1105
AciI CCGC 4 cut(s) 69, 653, 1105, 1129
AclWI GGATC 2 cut(s) 480, 1163
AcsI RAATTY 5 cut(s) 43, 216, 421, 688, 1208
AcuI CTGAAG 1 cut(s) 1047
AdeI CACNNNGTG 1 cut(s) 777
AfaI GTAC 6 cut(s) 112, 781, 808, 862, 891, 943
AfiI CCNNNNNNNGG 2 cut(s) 809, 1162
AflIII ACRYGT 1 cut(s) 107
AgsI TTSAA 6 cut(s) 34, 315, 638, 693, 843, 1123
AjiI CACGTC 1 cut(s) 1075
AjnI CCWGG 2 cut(s) 622, 851
AluBI AGCT 6 cut(s) 88, 161, 677, 848, 1058, 1161
AluI AGCT 6 cut(s) 88, 161, 677, 848, 1058, 1161
Alw21I GWGCWC 1 cut(s) 931
Alw26I GTCTC 2 cut(s) 907, 930
Alw44I GTGCAC 1 cut(s) 927
AlwI GGATC 2 cut(s) 480, 1163
Ama87I CYCGRG 1 cut(s) 1156
AoxI GGCC 2 cut(s) 290, 492
ApaLI GTGCAC 1 cut(s) 927
ApeKI GCWGC 4 cut(s) 85, 768, 848, 1058
ApoI RAATTY 5 cut(s) 43, 216, 421, 688, 1208
AspA2I CCTAGG 1 cut(s) 269
AspS9I GGNCC 1 cut(s) 797
AsuC2I CCSGG 1 cut(s) 811
AvaI CYCGRG 1 cut(s) 1156
AvaII GGWCC 1 cut(s) 797
AvrII CCTAGG 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 931
BauI CACGAG 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 1158
Bbv12I GWGCWC 1 cut(s) 931
BbvI GCAGC 4 cut(s) 97, 780, 835, 1045
BccI CCATC 4 cut(s) 50, 443, 455, 891
BciT130I CCWGG 2 cut(s) 624, 853
BcnI CCSGG 1 cut(s) 811
BcoDI GTCTC 2 cut(s) 907, 930
BfaI CTAG 1 cut(s) 270
BglII AGATCT 1 cut(s) 1223
BisI GCNGC 4 cut(s) 86, 769, 849, 1059
BlnI CCTAGG 1 cut(s) 269
BlsI GCNGC 4 cut(s) 87, 770, 850, 1060
Bme1390I CCNGG 3 cut(s) 624, 811, 853
Bme18I GGWCC 1 cut(s) 797
BmeT110I CYCGRG 1 cut(s) 1156
BmgBI CACGTC 1 cut(s) 1075
BmgT120I GGNCC 1 cut(s) 797
BmiI GGNNCC 3 cut(s) 798, 831, 1032
BmrFI CCNGG 3 cut(s) 624, 811, 853
BmsI GCATC 4 cut(s) 382, 559, 691, 877
BpiI GAAGAC 1 cut(s) 1158
BpmI CTGGAG 1 cut(s) 680
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 811
BsaAI YACGTR 1 cut(s) 580
BsaBI GATNNNNATC 1 cut(s) 702
BsaI GGTCTC 1 cut(s) 930
BsaJI CCNNGG 1 cut(s) 269
BsaWI WCCGGW 1 cut(s) 361
Bsc4I CCNNNNNNNGG 2 cut(s) 809, 1162
Bse1I ACTGG 1 cut(s) 663
Bse8I GATNNNNATC 1 cut(s) 702
BseBI CCWGG 2 cut(s) 624, 853
BseDI CCNNGG 1 cut(s) 269
BseGI GGATG 6 cut(s) 33, 550, 598, 619, 682, 902
BseJI GATNNNNATC 1 cut(s) 702
BseLI CCNNNNNNNGG 2 cut(s) 809, 1162
BseMII CTCAG 2 cut(s) 683, 1026
BseNI ACTGG 1 cut(s) 663
BseSI GKGCMC 1 cut(s) 931
BseXI GCAGC 4 cut(s) 97, 780, 835, 1045
BseYI CCCAGC 1 cut(s) 1161
Bsh1236I CGCG 1 cut(s) 1105
BshFI GGCC 2 cut(s) 292, 494
BsiHKAI GWGCWC 1 cut(s) 931
BsiHKCI CYCGRG 1 cut(s) 1156
BsiSI CCGG 2 cut(s) 362, 810
BslFI GGGAC 2 cut(s) 669, 810
BslI CCNNNNNNNGG 2 cut(s) 809, 1162
BsmAI GTCTC 2 cut(s) 907, 930
BsmFI GGGAC 2 cut(s) 669, 810
BsnI GGCC 2 cut(s) 292, 494
Bso31I GGTCTC 1 cut(s) 930
BsoBI CYCGRG 1 cut(s) 1156
Bsp1286I GDGCHC 1 cut(s) 931
Bsp1407I TGTACA 2 cut(s) 779, 889
Bsp143I GATC 4 cut(s) 472, 883, 1168, 1223
BspACI CCGC 4 cut(s) 69, 653, 1105, 1129
BspANI GGCC 2 cut(s) 292, 494
BspCNI CTCAG 2 cut(s) 682, 1027
BspFNI CGCG 1 cut(s) 1105
BspLI GGNNCC 3 cut(s) 798, 831, 1032
BspPI GGATC 2 cut(s) 480, 1163
BspTNI GGTCTC 1 cut(s) 930
BsrGI TGTACA 2 cut(s) 779, 889
BsrI ACTGG 1 cut(s) 663
BssECI CCNNGG 1 cut(s) 269
BssMI GATC 4 cut(s) 472, 883, 1168, 1223
BssSI CACGAG 1 cut(s) 252
BssT1I CCWWGG 1 cut(s) 269
Bst2BI CACGAG 1 cut(s) 252
Bst2UI CCWGG 2 cut(s) 624, 853
Bst4CI ACNGT 5 cut(s) 115, 208, 245, 344, 941
Bst6I CTCTTC 2 cut(s) 742, 1037
BstAPI GCANNNNNTGC 1 cut(s) 1075
BstAUI TGTACA 2 cut(s) 779, 889
BstBAI YACGTR 1 cut(s) 580
BstC8I GCNNGC 2 cut(s) 354, 675
BstDEI CTNAG 2 cut(s) 669, 1035
BstF5I GGATG 6 cut(s) 33, 550, 598, 619, 682, 902
BstFNI CGCG 1 cut(s) 1105
BstKTI GATC 4 cut(s) 475, 886, 1171, 1226
BstMAI GTCTC 2 cut(s) 907, 930
BstMBI GATC 4 cut(s) 472, 883, 1168, 1223
BstMWI GCNNNNNNNGC 4 cut(s) 679, 1075, 1102, 1191
BstNI CCWGG 2 cut(s) 624, 853
BstNSI RCATGY 1 cut(s) 111
BstSCI CCNGG 3 cut(s) 622, 809, 851
BstSLI GKGCMC 1 cut(s) 931
BstSNI TACGTA 1 cut(s) 580
BstUI CGCG 1 cut(s) 1105
BstV1I GCAGC 4 cut(s) 97, 780, 835, 1045
BstV2I GAAGAC 1 cut(s) 1158
BstX2I RGATCY 2 cut(s) 472, 1223
BstXI CCANNNNNNTGG 3 cut(s) 18, 449, 952
BstYI RGATCY 2 cut(s) 472, 1223
BsuRI GGCC 2 cut(s) 292, 494
BtrI CACGTC 1 cut(s) 1075
BtsCI GGATG 6 cut(s) 33, 550, 598, 619, 682, 902
Cac8I GCNNGC 2 cut(s) 354, 675
Cfr13I GGNCC 1 cut(s) 797
CseI GACGC 1 cut(s) 191
Csp6I GTAC 6 cut(s) 111, 780, 807, 861, 890, 942
CspCI CAANNNNNGTGG 2 cut(s) 506, 541
CviAII CATG 6 cut(s) 18, 108, 804, 826, 893, 1070
CviQI GTAC 6 cut(s) 111, 780, 807, 861, 890, 942
DdeI CTNAG 2 cut(s) 669, 1035
DpnI GATC 4 cut(s) 474, 885, 1170, 1225
DpnII GATC 4 cut(s) 472, 883, 1168, 1223
DraI TTTAAA 1 cut(s) 1177
DraIII CACNNNGTG 1 cut(s) 777
Eam1104I CTCTTC 2 cut(s) 742, 1037
EarI CTCTTC 2 cut(s) 742, 1037
Eco105I TACGTA 1 cut(s) 580
Eco130I CCWWGG 1 cut(s) 269
Eco147I AGGCCT 1 cut(s) 292
Eco31I GGTCTC 1 cut(s) 930
Eco32I GATATC 1 cut(s) 666
Eco47I GGWCC 1 cut(s) 797
Eco57I CTGAAG 1 cut(s) 1047
Eco88I CYCGRG 1 cut(s) 1156
EcoRI GAATTC 1 cut(s) 43
EcoRII CCWGG 2 cut(s) 622, 851
EcoRV GATATC 1 cut(s) 666
EcoT14I CCWWGG 1 cut(s) 269
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 6 cut(s) 21, 111, 807, 829, 896, 1073
FaqI GGGAC 2 cut(s) 669, 810
FatI CATG 6 cut(s) 17, 107, 803, 825, 892, 1069
FauI CCCGC 2 cut(s) 646, 1098
FblI GTMKAC 1 cut(s) 1019
Fnu4HI GCNGC 4 cut(s) 86, 769, 849, 1059
FokI GGATG 6 cut(s) 40, 537, 605, 606, 669, 909
Fsp4HI GCNGC 4 cut(s) 86, 769, 849, 1059
FspBI CTAG 1 cut(s) 270
GluI GCNGC 4 cut(s) 86, 769, 849, 1059
GsaI CCCAGC 1 cut(s) 1165
GsuI CTGGAG 1 cut(s) 680
HaeIII GGCC 2 cut(s) 292, 494
HapII CCGG 2 cut(s) 362, 810
HgaI GACGC 1 cut(s) 191
Hin1II CATG 6 cut(s) 21, 111, 807, 829, 896, 1073
HincII GTYRAC 3 cut(s) 303, 1020, 1101
HindII GTYRAC 3 cut(s) 303, 1020, 1101
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 1 cut(s) 193
HpaII CCGG 2 cut(s) 362, 810
Hpy166II GTNNAC 7 cut(s) 248, 303, 780, 929, 982, 1020, 1101
Hpy188I TCNGA 2 cut(s) 55, 277
Hpy188III TCNNGA 8 cut(s) 34, 231, 416, 476, 554, 843, 905, 1034
Hpy8I GTNNAC 7 cut(s) 248, 303, 780, 929, 982, 1020, 1101
Hpy99I CGWCG 1 cut(s) 1021
HpyAV CCTTC 2 cut(s) 815, 1129
HpyCH4III ACNGT 5 cut(s) 115, 208, 245, 344, 941
HpyCH4IV ACGT 2 cut(s) 579, 1074
HpyCH4V TGCA 3 cut(s) 550, 682, 929
HpyF10VI GCNNNNNNNGC 4 cut(s) 679, 1075, 1102, 1191
HpyF3I CTNAG 2 cut(s) 669, 1035
HpySE526I ACGT 2 cut(s) 579, 1074
Hsp92II CATG 6 cut(s) 21, 111, 807, 829, 896, 1073
Kzo9I GATC 4 cut(s) 472, 883, 1168, 1223
LmnI GCTCC 1 cut(s) 835
Lsp1109I GCAGC 4 cut(s) 97, 780, 835, 1045
LweI GCATC 4 cut(s) 382, 559, 691, 877
MaeI CTAG 1 cut(s) 270
MaeII ACGT 2 cut(s) 579, 1074
MaeIII GTNAC 3 cut(s) 103, 344, 485
MalI GATC 4 cut(s) 474, 885, 1170, 1225
MboI GATC 4 cut(s) 472, 883, 1168, 1223
MboII GAAGA 8 cut(s) 88, 404, 639, 759, 1051, 1054, 1160, 1163
MflI RGATCY 2 cut(s) 472, 1223
MhlI GDGCHC 1 cut(s) 931
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 6 cut(s) 261, 303, 484, 589, 758, 1086
MseI TTAA 4 cut(s) 320, 372, 1176, 1228
MslI CAYNNNNRTG 2 cut(s) 22, 447
MspA1I CMGCKG 2 cut(s) 88, 1129
MspI CCGG 2 cut(s) 362, 810
MspR9I CCNGG 3 cut(s) 624, 811, 853
MvaI CCWGG 2 cut(s) 624, 853
MvnI CGCG 1 cut(s) 1105
MwoI GCNNNNNNNGC 4 cut(s) 679, 1075, 1102, 1191
NciI CCSGG 1 cut(s) 811
NdeII GATC 4 cut(s) 472, 883, 1168, 1223
NlaIII CATG 6 cut(s) 21, 111, 807, 829, 896, 1073
NlaIV GGNNCC 3 cut(s) 798, 831, 1032
NspI RCATGY 1 cut(s) 111
PceI AGGCCT 1 cut(s) 292
PciI ACATGT 1 cut(s) 107
PfeI GAWTC 1 cut(s) 193
PfoI TCCNGGA 1 cut(s) 622
PkrI GCNGC 4 cut(s) 87, 770, 850, 1060
Ppu21I YACGTR 1 cut(s) 580
PscI ACATGT 1 cut(s) 107
PsiI TTATAA 1 cut(s) 1115
Psp6I CCWGG 2 cut(s) 622, 851
PspFI CCCAGC 1 cut(s) 1161
PspGI CCWGG 2 cut(s) 622, 851
PspN4I GGNNCC 3 cut(s) 798, 831, 1032
PspPI GGNCC 1 cut(s) 797
PsuI RGATCY 2 cut(s) 472, 1223
PvuII CAGCTG 1 cut(s) 88
RsaI GTAC 6 cut(s) 112, 781, 808, 862, 891, 943
RsaNI GTAC 6 cut(s) 111, 780, 807, 861, 890, 942
RseI CAYNNNNRTG 2 cut(s) 22, 447
SalI GTCGAC 1 cut(s) 1018
SaqAI TTAA 4 cut(s) 320, 372, 1176, 1228
SatI GCNGC 4 cut(s) 86, 769, 849, 1059
Sau3AI GATC 4 cut(s) 472, 883, 1168, 1223
Sau96I GGNCC 1 cut(s) 797
ScrFI CCNGG 3 cut(s) 624, 811, 853
SduI GDGCHC 1 cut(s) 931
SfaNI GCATC 4 cut(s) 382, 559, 691, 877
SinI GGWCC 1 cut(s) 797
SmiMI CAYNNNNRTG 2 cut(s) 22, 447
SmlI CTYRAG 2 cut(s) 476, 1219
SmoI CTYRAG 2 cut(s) 476, 1219
SnaBI TACGTA 1 cut(s) 580
SseBI AGGCCT 1 cut(s) 292
SsiI CCGC 4 cut(s) 69, 653, 1105, 1129
SspMI CTAG 1 cut(s) 270
StuI AGGCCT 1 cut(s) 292
StyD4I CCNGG 3 cut(s) 622, 809, 851
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 5 cut(s) 115, 208, 245, 344, 941
TaiI ACGT 2 cut(s) 582, 1077
TaqI TCGA 4 cut(s) 230, 327, 1019, 1212
TatI WGTACW 3 cut(s) 779, 860, 889
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 4 cut(s) 320, 372, 1176, 1228
Tru9I TTAA 4 cut(s) 320, 372, 1176, 1228
TseI GCWGC 4 cut(s) 85, 768, 848, 1058
TspDTI ATGAA 6 cut(s) 17, 89, 95, 115, 608, 731
TspGWI ACGGA 1 cut(s) 188
VneI GTGCAC 1 cut(s) 927
VpaK11BI GGWCC 1 cut(s) 797
XapI RAATTY 5 cut(s) 43, 216, 421, 688, 1208
XceI RCATGY 1 cut(s) 111
XmaJI CCTAGG 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 1019
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.