Rorug01G0182100

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001643
Physical Location & Seq
Forward (+)
25076819 .. 25090149
13331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0182100.1

Sequence Viewer

Length: 408 bp
ATGGCAACGACGGCGTGTTTCATTATCGTGAGCAGAAACGACATCCCCATTTACGAAGCTGAAGTGGGATCTGCGGCCAAGAGAGAAGATGCTGCTCAGCTTCACCAGTTCATATTACATGCAGCTCTGGATATTGTTCAGGACCTTGCTTGGACTACTAGTGCCATGTATTTGAAAGCAGTAGACCGCTTTAATGATTTGGTGGTTTCAGCCTATGTTACAGCTGGTCATACACGACTTATGCTGCTTCATGACTCCCGGAATGATGACGGGATTAAGAGCTTTTTCCAAGAGGTTCATGAGCTTTATATCAAGACTCTTCTTAATCCGACCTACTTGCCTGGTTCACGAATAACATCATCACATTTTGATACCAAAGTTCGTGCCCTTGCACGAAAGTATCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

135

Amino Acids

15.23

Weight (kDa)

6.58

Isoelectric Point (pI)

22.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sedlin_N PF04628 9 - 133 3.2e-45 Sedlin, N-terminal conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 183
AciI CCGC 2 cut(s) 74, 187
AclWI GGATC 1 cut(s) 76
AcoI YGGCCR 1 cut(s) 75
AcuI CTGAAG 1 cut(s) 81
AgsI TTSAA 1 cut(s) 175
AhlI ACTAGT 1 cut(s) 158
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 6 cut(s) 59, 100, 125, 224, 282, 304
AluI AGCT 6 cut(s) 59, 100, 125, 224, 282, 304
AlwI GGATC 1 cut(s) 76
AoxI GGCC 1 cut(s) 75
ApeKI GCWGC 3 cut(s) 92, 122, 244
AspS9I GGNCC 1 cut(s) 142
AsuC2I CCSGG 1 cut(s) 259
AsuHPI GGTGA 1 cut(s) 95
AvaII GGWCC 1 cut(s) 142
BaeGI GKGCMC 1 cut(s) 388
BaeI ACNNNNGTAYC 3 cut(s) 363, 383, 396
BbvI GCAGC 3 cut(s) 79, 134, 231
BceAI ACGGC 1 cut(s) 27
BcgI CGANNNNNNTGC 2 cut(s) 319, 353
BciT130I CCWGG 1 cut(s) 342
BcnI CCSGG 1 cut(s) 259
BcuI ACTAGT 1 cut(s) 158
BfaI CTAG 1 cut(s) 159
BfmI CTRYAG 1 cut(s) 404
BisI GCNGC 4 cut(s) 75, 93, 123, 245
BlpI GCTNAGC 1 cut(s) 96
BlsI GCNGC 4 cut(s) 76, 94, 124, 246
Bme1390I CCNGG 2 cut(s) 259, 342
Bme18I GGWCC 1 cut(s) 142
BmgT120I GGNCC 1 cut(s) 142
BmrFI CCNGG 2 cut(s) 259, 342
BmsI GCATC 1 cut(s) 79
Bpu1102I GCTNAGC 1 cut(s) 96
BpuMI CCSGG 1 cut(s) 259
Bse1I ACTGG 1 cut(s) 106
BseBI CCWGG 1 cut(s) 342
BseGI GGATG 1 cut(s) 42
BseMII CTCAG 1 cut(s) 110
BseNI ACTGG 1 cut(s) 106
BseSI GKGCMC 1 cut(s) 388
BseXI GCAGC 3 cut(s) 79, 134, 231
BshFI GGCC 1 cut(s) 77
BsiSI CCGG 1 cut(s) 259
BsnI GGCC 1 cut(s) 77
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 1 cut(s) 68
Bsp1720I GCTNAGC 1 cut(s) 96
BspACI CCGC 2 cut(s) 74, 187
BspANI GGCC 1 cut(s) 77
BspCNI CTCAG 1 cut(s) 109
BspHI TCATGA 2 cut(s) 250, 298
BspPI GGATC 1 cut(s) 76
BsrI ACTGG 1 cut(s) 106
BssMI GATC 1 cut(s) 68
Bst2UI CCWGG 1 cut(s) 342
Bst6I CTCTTC 1 cut(s) 324
BstDEI CTNAG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 42
BstKTI GATC 1 cut(s) 71
BstMBI GATC 1 cut(s) 68
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 1 cut(s) 122
BstSCI CCNGG 2 cut(s) 257, 340
BstSFI CTRYAG 1 cut(s) 404
BstSLI GKGCMC 1 cut(s) 388
BstV1I GCAGC 3 cut(s) 79, 134, 231
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
BsuRI GGCC 1 cut(s) 77
BtsCI GGATG 1 cut(s) 42
CciI TCATGA 2 cut(s) 250, 298
Cfr13I GGNCC 1 cut(s) 142
CviAII CATG 4 cut(s) 119, 166, 251, 299
CviJI RGCY 8 cut(s) 59, 77, 100, 125, 212, 224, 282, 304
CviKI_1 RGCY 8 cut(s) 59, 77, 100, 125, 212, 224, 282, 304
DdeI CTNAG 1 cut(s) 96
DpnI GATC 1 cut(s) 70
DpnII GATC 1 cut(s) 68
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 142
Eco57I CTGAAG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 340
FaeI CATG 4 cut(s) 122, 169, 254, 302
FaiI YATR 9 cut(s) 113, 120, 167, 216, 231, 242, 252, 300, 309
FatI CATG 4 cut(s) 118, 165, 250, 298
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 4 cut(s) 75, 93, 123, 245
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 4 cut(s) 75, 93, 123, 245
FspBI CTAG 1 cut(s) 159
GluI GCNGC 4 cut(s) 75, 93, 123, 245
HaeIII GGCC 1 cut(s) 77
HapII CCGG 1 cut(s) 259
Hin1II CATG 4 cut(s) 122, 169, 254, 302
HinfI GANTC 2 cut(s) 254, 316
HpaII CCGG 1 cut(s) 259
HphI GGTGA 1 cut(s) 95
Hpy166II GTNNAC 2 cut(s) 184, 347
Hpy188I TCNGA 1 cut(s) 330
Hpy188III TCNNGA 7 cut(s) 28, 128, 140, 251, 299, 313, 348
Hpy8I GTNNAC 2 cut(s) 184, 347
Hpy99I CGWCG 1 cut(s) 13
HpyCH4V TGCA 2 cut(s) 122, 392
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 96
Hsp92II CATG 4 cut(s) 122, 169, 254, 302
Kzo9I GATC 1 cut(s) 68
LpnPI CCDG 7 cut(s) 113, 119, 125, 210, 272, 327, 354
Lsp1109I GCAGC 3 cut(s) 79, 134, 231
LweI GCATC 1 cut(s) 79
MaeI CTAG 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 217
MalI GATC 1 cut(s) 70
MboI GATC 1 cut(s) 68
MboII GAAGA 2 cut(s) 98, 311
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 1 cut(s) 388
MlyI GAGTC 2 cut(s) 248, 310
MmeI TCCRAC 1 cut(s) 353
MnlI CCTC 1 cut(s) 286
MseI TTAA 3 cut(s) 192, 276, 324
MslI CAYNNNNRTG 1 cut(s) 26
MspA1I CMGCKG 1 cut(s) 224
MspI CCGG 1 cut(s) 259
MspR9I CCNGG 2 cut(s) 259, 342
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 1 cut(s) 259
NdeII GATC 1 cut(s) 68
NlaIII CATG 4 cut(s) 122, 169, 254, 302
NspI RCATGY 1 cut(s) 122
PagI TCATGA 2 cut(s) 250, 298
PfoI TCCNGGA 1 cut(s) 257
PkrI GCNGC 4 cut(s) 76, 94, 124, 246
PleI GAGTC 2 cut(s) 248, 310
PpsI GAGTC 2 cut(s) 248, 310
PpuMI RGGWCCY 1 cut(s) 142
Psp5II RGGWCCY 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspPI GGNCC 1 cut(s) 142
PspPPI RGGWCCY 1 cut(s) 142
PsuI RGATCY 1 cut(s) 68
PvuII CAGCTG 1 cut(s) 224
RseI CAYNNNNRTG 1 cut(s) 26
SaqAI TTAA 3 cut(s) 192, 276, 324
SatI GCNGC 4 cut(s) 75, 93, 123, 245
Sau3AI GATC 1 cut(s) 68
Sau96I GGNCC 1 cut(s) 142
SchI GAGTC 2 cut(s) 248, 310
ScrFI CCNGG 2 cut(s) 259, 342
SduI GDGCHC 1 cut(s) 388
SetI ASST 9 cut(s) 61, 102, 127, 147, 226, 284, 297, 306, 335
SfaNI GCATC 1 cut(s) 79
SfcI CTRYAG 1 cut(s) 404
SinI GGWCC 1 cut(s) 142
SmiMI CAYNNNNRTG 1 cut(s) 26
SpeI ACTAGT 1 cut(s) 158
SsiI CCGC 2 cut(s) 74, 187
SspMI CTAG 1 cut(s) 159
StyD4I CCNGG 2 cut(s) 257, 340
TauI GCSGC 1 cut(s) 77
Tru1I TTAA 3 cut(s) 192, 276, 324
Tru9I TTAA 3 cut(s) 192, 276, 324
TseI GCWGC 3 cut(s) 92, 122, 244
TspDTI ATGAA 4 cut(s) 10, 100, 239, 287
VpaK11BI GGWCC 1 cut(s) 142
XceI RCATGY 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.