Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0003515

Physical Location & Seq

Genomic Coordinates Forward (+)

26539002 .. 26541651

Gene Structure 2650 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1123800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1206 bp

ATGATTGAAGTATGTGGTGATGTGCAAGATATGGATACGAGCTTGGGTGGGAGAAAAATTTGTGGAGATGGACCGTGCGGATTTTCAGATGCTAAAACCAGTTCCAAAGATGCACAGGAACGGTCAGCGTCCATGCGGAAGCTTTTGATAGCGGTTATCCTGTGTGTCATTTTCATGAGTGTGGAGGTTGTTGGGGGTATCAAAGCCAACAGTCTTGCAATTCTCACTGATGCGGCTCATTTATTGTCAGATGTTGCTGCATTTGCAATTTCTTTATTCTCACTTTGGGCATCAGGATGGGAGGCGACTCCTCGTCAGTCTTACGGTTTCTTCCGGATTGAAATACTTGGTGCTCTTGTTTCCATTCAGATGATTTGGCTGCTTGCTGGGATCCTTGTTTATGAAGCCATTGCAAGACTTATCCATGACACTGGTGAAGTTCAGGGCTTTCTCATGTTTGTTGTTTCTGCGTTTGGTTTAGTGGTGAATATCGCCATGGCACTCTTATTAGGTCATGATCATGGGCATGATCACGGACATGGGCATGGCCATGGTGTCCATGGTCATGGACATGAGGATCATGATGAGACACATAACCATGGGATTACTGTGACCACACATCACTCTCATCATCATATTCATCATCCTGTGGTGCACTCGAATCATGATGACAAACACCATCATACCGAGGTAGTCAACATCTCAGAACCTCTGCTCGGTCATAGCTCTGAAGGTGAAAAGAAACAAGAAGGCAAAGGGAAGAAGCAACGAAACGTCAATCTGCAGGGGGCTTATCTTCATGTACTTGGCGATGCCATTCAGAGTATTGGAGTGATGATTGGTGGAGGGATTATCTGGTACAAGCCTGAGTGGAAGATTATTGATCTGATATGTACCCTTGTATTTTCAATAATCGTGCTGGCGACAACAATCCGGATGTTGAGGAACATTTTGGAGGTTTTGATGGAGAGTACTCCTAGAGAGATTGATGCCACAAAGATTGAGAAAGGTCTCTGCGAGATGGACGAGGTAATTGCCATTCACGAGCTGCACATATGGGCAATAACTGTTGGGAAGGTGTTAATGGCTTGTCATGTTATCGTTAAGTCGGATGCTAATGCTGATGTGGTGCTGGAAAAGGTTATAGACTACATCAAGAGAGAATATAATATCAGTCATGTAACGATTCAGATAGAGCGACAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005385 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0022857 GO:0022890 GO:0030001 GO:0032879 GO:0034220 GO:0042592 GO:0046873 GO:0046915 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051179 GO:0051234 GO:0055085 GO:0061088 GO:0065007 GO:0065008 GO:0070838 GO:0071577 GO:0072509 GO:0072511 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux

Protein Analysis

402

Amino Acids

44.13

Weight (kDa)

6.05

Isoelectric Point (pI)

34.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	48 - 322	1.5e-37	Cation efflux transmembrane domain
ZT_dimer	PF16916	326 - 400	3.1e-13	Cation efflux protein, cytoplasmic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003515)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58810 AT3G58810
fragaria_vesca	FvH4_5g10450 FvH4_5g10450 FvH4_5g10450
malus_domestica	MD06G1123800.v1.1 MD13G1032200.v1.1 MD14G1140000.v1.1 MD14G1140800.v1.1
prunus_persica	Prupe.5G134300_v2.0.a1 Prupe.5G134300_v2.0.a1 Prupe.5G134300_v2.0.a1
pyrus_communis	pycom14g11570
rosa_chinensis	RchiOBHm_Chr4g0431771 RchiOBHm_Chr7g0187761
rosa_laevigata	RLG00000004758 RLG00000006891
rosa_multiflora	Rmu_sc0001459.1_g000019 Rmu_sc0004483.1_g000029
rosa_roxburghii	Rroxscaffold_3G00267100 Rroxscaffold_5G00373170 Rroxscaffold_5G00373180
rosa_rugosa	Rorug04G0252300 Rorug06G0484000.1
rosa_samantha	Rh4AG308700 Rh4BG316500 Rh4CG331900 Rh4DG311400 Rh7AG089100 Rh7BG090900 Rh7CG089900 Rh7DG091500
rosa_wichuraiana	Rw0G003020 Rw4G026780 Rw7G007690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccIII	TCCGGA	2 cut(s)	333, 933
AciI	CCGC	4 cut(s)	78, 136, 152, 233
AclWI	GGATC	3 cut(s)	385, 398, 585
AcoI	YGGCCR	1 cut(s)	547
AcsI	RAATTY	1 cut(s)	57
AcuI	CTGAAG	1 cut(s)	750
AfaI	GTAC	4 cut(s)	804, 860, 895, 973
AfiI	CCNNNNNNNGG	1 cut(s)	716
AgsI	TTSAA	3 cut(s)	8, 341, 909
AhdI	GACNNNNNGTC	1 cut(s)	312
AluBI	AGCT	4 cut(s)	42, 142, 726, 1048
AluI	AGCT	4 cut(s)	42, 142, 726, 1048
Alw21I	GWGCWC	2 cut(s)	355, 657
Alw26I	GTCTC	2 cut(s)	581, 1016
Alw44I	GTGCAC	1 cut(s)	653
AlwI	GGATC	3 cut(s)	385, 398, 585
Aor13HI	TCCGGA	2 cut(s)	333, 933
AoxI	GGCC	1 cut(s)	547
ApaLI	GTGCAC	1 cut(s)	653
ApeKI	GCWGC	3 cut(s)	257, 379, 1048
ApoI	RAATTY	1 cut(s)	57
AspS9I	GGNCC	1 cut(s)	71
AsuHPI	GGTGA	4 cut(s)	29, 446, 496, 746
AvaII	GGWCC	1 cut(s)	71
BaeGI	GKGCMC	1 cut(s)	657
BalI	TGGCCA	1 cut(s)	549
BamHI	GGATCC	1 cut(s)	390
BauI	CACGAG	1 cut(s)	1043
Bbv12I	GWGCWC	2 cut(s)	355, 657
BbvI	GCAGC	3 cut(s)	244, 366, 1035
BccI	CCATC	5 cut(s)	62, 291, 687, 958, 1015
BcgI	CGANNNNNNTGC	2 cut(s)	1016, 1050
BciVI	GTATCC	1 cut(s)	28
BclI	TGATCA	2 cut(s)	517, 529
BcoDI	GTCTC	2 cut(s)	581, 1016
BfaI	CTAG	1 cut(s)	978
BfmI	CTRYAG	1 cut(s)	782
BfuI	GTATCC	1 cut(s)	28
BisI	GCNGC	4 cut(s)	234, 258, 380, 1049
BlsI	GCNGC	4 cut(s)	235, 259, 381, 1050
BmcAI	AGTACT	1 cut(s)	973
Bme18I	GGWCC	1 cut(s)	71
BmeRI	GACNNNNNGTC	1 cut(s)	312
BmgT120I	GGNCC	1 cut(s)	71
BmiI	GGNNCC	1 cut(s)	392
BmsI	GCATC	7 cut(s)	79, 100, 220, 299, 802, 979, 1102
BsaI	GGTCTC	1 cut(s)	1016
BsaJI	CCNNGG	5 cut(s)	495, 550, 559, 598, 687
BsaWI	WCCGGW	2 cut(s)	333, 933
Bsc4I	CCNNNNNNNGG	1 cut(s)	716
Bse1I	ACTGG	2 cut(s)	99, 436
Bse3DI	GCAATG	1 cut(s)	408
BseAI	TCCGGA	2 cut(s)	333, 933
BseDI	CCNNGG	5 cut(s)	495, 550, 559, 598, 687
BseGI	GGATG	4 cut(s)	302, 643, 942, 1117
BseLI	CCNNNNNNNGG	1 cut(s)	716
BseMI	GCAATG	1 cut(s)	408
BseMII	CTCAG	2 cut(s)	717, 858
BseNI	ACTGG	2 cut(s)	99, 436
BseRI	GAGGAG	1 cut(s)	300
BseSI	GKGCMC	1 cut(s)	657
BseXI	GCAGC	3 cut(s)	244, 366, 1035
BseYI	CCCAGC	1 cut(s)	386
BsgI	GTGCAG	1 cut(s)	1034
BshFI	GGCC	1 cut(s)	549
BsiHKAI	GWGCWC	2 cut(s)	355, 657
BsiSI	CCGG	2 cut(s)	334, 934
BslI	CCNNNNNNNGG	1 cut(s)	716
BsmAI	GTCTC	2 cut(s)	581, 1016
BsnI	GGCC	1 cut(s)	549
Bso31I	GGTCTC	1 cut(s)	1016
Bsp1286I	GDGCHC	2 cut(s)	355, 657
Bsp13I	TCCGGA	2 cut(s)	333, 933
Bsp143I	GATC	5 cut(s)	390, 517, 529, 577, 883
Bsp19I	CCATGG	4 cut(s)	495, 550, 559, 598
BspACI	CCGC	4 cut(s)	78, 136, 152, 233
BspANI	GGCC	1 cut(s)	549
BspCNI	CTCAG	2 cut(s)	716, 859
BspEI	TCCGGA	2 cut(s)	333, 933
BspHI	TCATGA	4 cut(s)	174, 514, 580, 664
BspLI	GGNNCC	1 cut(s)	392
BspMAI	CTGCAG	1 cut(s)	786
BspPI	GGATC	3 cut(s)	385, 398, 585
BspTNI	GGTCTC	1 cut(s)	1016
BsrDI	GCAATG	1 cut(s)	408
BsrI	ACTGG	2 cut(s)	99, 436
BssECI	CCNNGG	5 cut(s)	495, 550, 559, 598, 687
BssMI	GATC	5 cut(s)	390, 517, 529, 577, 883
BssSI	CACGAG	1 cut(s)	1043
BssT1I	CCWWGG	4 cut(s)	495, 550, 559, 598
Bst2BI	CACGAG	1 cut(s)	1043
Bst4CI	ACNGT	7 cut(s)	75, 123, 212, 326, 610, 1069, 1203
BstC8I	GCNNGC	2 cut(s)	384, 921
BstDEI	CTNAG	2 cut(s)	703, 867
BstDSI	CCRYGG	4 cut(s)	495, 550, 559, 598
BstF5I	GGATG	4 cut(s)	302, 643, 942, 1117
BstKTI	GATC	5 cut(s)	393, 520, 532, 580, 886
BstMAI	GTCTC	2 cut(s)	581, 1016
BstMBI	GATC	5 cut(s)	390, 517, 529, 577, 883
BstMWI	GCNNNNNNNGC	1 cut(s)	263
BstSFI	CTRYAG	1 cut(s)	782
BstSLI	GKGCMC	1 cut(s)	657
BstV1I	GCAGC	3 cut(s)	244, 366, 1035
BstX2I	RGATCY	1 cut(s)	390
BstXI	CCANNNNNNTGG	2 cut(s)	431, 566
BstYI	RGATCY	1 cut(s)	390
BsuI	GTATCC	1 cut(s)	28
BsuRI	GGCC	1 cut(s)	549
BtgI	CCRYGG	4 cut(s)	495, 550, 559, 598
BtgZI	GCGATG	1 cut(s)	825
BtsCI	GGATG	4 cut(s)	302, 643, 942, 1117
BtsIMutI	CAGTG	2 cut(s)	225, 429
Cac8I	GCNNGC	2 cut(s)	384, 921
CciI	TCATGA	4 cut(s)	174, 514, 580, 664
Cfr13I	GGNCC	1 cut(s)	71
CseI	GACGC	1 cut(s)	117
Csp6I	GTAC	4 cut(s)	803, 859, 894, 972
CspCI	CAANNNNNGTGG	2 cut(s)	982, 1017
CviQI	GTAC	4 cut(s)	803, 859, 894, 972
DdeI	CTNAG	2 cut(s)	703, 867
DpnI	GATC	5 cut(s)	392, 519, 531, 579, 885
DpnII	GATC	5 cut(s)	390, 517, 529, 577, 883
DriI	GACNNNNNGTC	1 cut(s)	312
EaeI	YGGCCR	1 cut(s)	547
Eam1105I	GACNNNNNGTC	1 cut(s)	312
Eco130I	CCWWGG	4 cut(s)	495, 550, 559, 598
Eco31I	GGTCTC	1 cut(s)	1016
Eco47I	GGWCC	1 cut(s)	71
Eco57I	CTGAAG	1 cut(s)	750
EcoT14I	CCWWGG	4 cut(s)	495, 550, 559, 598
ErhI	CCWWGG	4 cut(s)	495, 550, 559, 598
FauNDI	CATATG	1 cut(s)	1055
FbaI	TGATCA	2 cut(s)	517, 529
Fnu4HI	GCNGC	4 cut(s)	234, 258, 380, 1049
FokI	GGATG	4 cut(s)	309, 630, 949, 1124
Fsp4HI	GCNGC	4 cut(s)	234, 258, 380, 1049
FspBI	CTAG	1 cut(s)	978
GluI	GCNGC	4 cut(s)	234, 258, 380, 1049
GsaI	CCCAGC	1 cut(s)	390
HaeIII	GGCC	1 cut(s)	549
HapII	CCGG	2 cut(s)	334, 934
HgaI	GACGC	1 cut(s)	117
HincII	GTYRAC	1 cut(s)	697
HindII	GTYRAC	1 cut(s)	697
HindIII	AAGCTT	1 cut(s)	140
HinfI	GANTC	3 cut(s)	307, 661, 1186
HpaII	CCGG	2 cut(s)	334, 934
HphI	GGTGA	4 cut(s)	29, 446, 496, 746
Hpy166II	GTNNAC	2 cut(s)	655, 697
Hpy188I	TCNGA	9 cut(s)	88, 250, 369, 706, 730, 822, 888, 1111, 1191
Hpy188III	TCNNGA	9 cut(s)	175, 294, 334, 515, 581, 665, 934, 1043, 1156
Hpy8I	GTNNAC	2 cut(s)	655, 697
HpyAV	CCTTC	3 cut(s)	725, 743, 1069
HpyCH4III	ACNGT	7 cut(s)	75, 123, 212, 326, 610, 1069, 1203
HpyCH4IV	ACGT	1 cut(s)	774
HpyCH4V	TGCA	9 cut(s)	25, 113, 218, 260, 266, 413, 655, 784, 1051
HpyF10VI	GCNNNNNNNGC	1 cut(s)	263
HpyF3I	CTNAG	2 cut(s)	703, 867
HpySE526I	ACGT	1 cut(s)	774
Kpn2I	TCCGGA	2 cut(s)	333, 933
Ksp22I	TGATCA	2 cut(s)	517, 529
Kzo9I	GATC	5 cut(s)	390, 517, 529, 577, 883
Lsp1109I	GCAGC	3 cut(s)	244, 366, 1035
LweI	GCATC	7 cut(s)	79, 100, 220, 299, 802, 979, 1102
MaeI	CTAG	1 cut(s)	978
MaeII	ACGT	1 cut(s)	774
MaeIII	GTNAC	2 cut(s)	610, 1180
MalI	GATC	5 cut(s)	392, 519, 531, 579, 885
MboI	GATC	5 cut(s)	390, 517, 529, 577, 883
MboII	GAAGA	4 cut(s)	322, 772, 788, 886
MflI	RGATCY	1 cut(s)	390
MhlI	GDGCHC	2 cut(s)	355, 657
MlsI	TGGCCA	1 cut(s)	549
MluCI	AATT	4 cut(s)	57, 219, 267, 1032
MluNI	TGGCCA	1 cut(s)	549
MlyI	GAGTC	1 cut(s)	301
MmeI	TCCRAC	1 cut(s)	1089
Mox20I	TGGCCA	1 cut(s)	549
MroI	TCCGGA	2 cut(s)	333, 933
MscI	TGGCCA	1 cut(s)	549
MseI	TTAA	2 cut(s)	1082, 1104
Msp20I	TGGCCA	1 cut(s)	549
MspI	CCGG	2 cut(s)	334, 934
MwoI	GCNNNNNNNGC	1 cut(s)	263
NcoI	CCATGG	4 cut(s)	495, 550, 559, 598
NdeI	CATATG	1 cut(s)	1055
NdeII	GATC	5 cut(s)	390, 517, 529, 577, 883
NlaIV	GGNNCC	1 cut(s)	392
NmuCI	GTSAC	1 cut(s)	610
PagI	TCATGA	4 cut(s)	174, 514, 580, 664
PfeI	GAWTC	2 cut(s)	661, 1186
PkrI	GCNGC	4 cut(s)	235, 259, 381, 1050
PleI	GAGTC	1 cut(s)	301
PpsI	GAGTC	1 cut(s)	301
PspFI	CCCAGC	1 cut(s)	386
PspN4I	GGNNCC	1 cut(s)	392
PspPI	GGNCC	1 cut(s)	71
PstI	CTGCAG	1 cut(s)	786
PsuI	RGATCY	1 cut(s)	390
RsaI	GTAC	4 cut(s)	804, 860, 895, 973
RsaNI	GTAC	4 cut(s)	803, 859, 894, 972
SaqAI	TTAA	2 cut(s)	1082, 1104
SatI	GCNGC	4 cut(s)	234, 258, 380, 1049
Sau3AI	GATC	5 cut(s)	390, 517, 529, 577, 883
Sau96I	GGNCC	1 cut(s)	71
ScaI	AGTACT	1 cut(s)	973
SchI	GAGTC	1 cut(s)	301
SduI	GDGCHC	2 cut(s)	355, 657
SfaNI	GCATC	7 cut(s)	79, 100, 220, 299, 802, 979, 1102
SfcI	CTRYAG	1 cut(s)	782
SinI	GGWCC	1 cut(s)	71
Sse9I	AATT	4 cut(s)	57, 219, 267, 1032
SsiI	CCGC	4 cut(s)	78, 136, 152, 233
SspMI	CTAG	1 cut(s)	978
StyI	CCWWGG	4 cut(s)	495, 550, 559, 598
TaaI	ACNGT	7 cut(s)	75, 123, 212, 326, 610, 1069, 1203
TaiI	ACGT	1 cut(s)	777
TaqI	TCGA	1 cut(s)	659
TaqII	GACCGA	1 cut(s)	707
TasI	AATT	4 cut(s)	57, 219, 267, 1032
TatI	WGTACW	2 cut(s)	802, 971
TauI	GCSGC	1 cut(s)	236
TfiI	GAWTC	2 cut(s)	661, 1186
Tru1I	TTAA	2 cut(s)	1082, 1104
Tru9I	TTAA	2 cut(s)	1082, 1104
TscAI	CASTG	2 cut(s)	232, 436
TseFI	GTSAC	1 cut(s)	610
TseI	GCWGC	3 cut(s)	257, 379, 1048
Tsp45I	GTSAC	1 cut(s)	610
TspDTI	ATGAA	4 cut(s)	163, 417, 629, 788
TspGWI	ACGGA	1 cut(s)	549
TspRI	CASTG	2 cut(s)	232, 436
VneI	GTGCAC	1 cut(s)	653
VpaK11BI	GGWCC	1 cut(s)	71
XapI	RAATTY	1 cut(s)	57
XcmI	CCANNNNNNNNNTGG	1 cut(s)	557
XspI	CTAG	1 cut(s)	978
ZrmI	AGTACT	1 cut(s)	973

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.