Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003515

Physical Location & Seq

Genomic Coordinates Forward (+)

23084118 .. 23084562

Gene Structure 445 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1140800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 354 bp

ATGCGGAAGCTTTTGATAGCAGTTGTCCTCTGTGTCATTTTCATGAGTGTAGAGGTTGTTGGGGGTATCAAAGCTCACAGCCAAGCAATTCTCACCGATACGGCTCATTTATTGTCAGATGTTGTCGCATTTGCAATTTCTTTGTTCTCGCTCTGGCCATCAGGATGGGAGGGAACTCATCGTCAGTCTTACGGCTTCTTCCAGATTGAAATACTAGGTGCTCTTGTTTCCATTCAGATGATTTGGCTGCTTGCTGGATCCTTGTTTATGAAGCCATTGCAAGACTTATCAATGACACTGGCACGATCACGGACATGGACATGGCCATGGTGGGCATGGTCATGGACATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005385 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0022857 GO:0022890 GO:0030001 GO:0032879 GO:0034220 GO:0042592 GO:0046873 GO:0046915 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051179 GO:0051234 GO:0055085 GO:0061088 GO:0065007 GO:0065008 GO:0070838 GO:0071577 GO:0072509 GO:0072511 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux

Protein Analysis

118

Amino Acids

13.35

Weight (kDa)

7.98

Isoelectric Point (pI)

28.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	4 - 96	5.6e-19	Cation efflux transmembrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0003515)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58810 AT3G58810
fragaria_vesca	FvH4_5g10450 FvH4_5g10450 FvH4_5g10450
malus_domestica	MD06G1123800.v1.1 MD13G1032200.v1.1 MD14G1140000.v1.1 MD14G1140800.v1.1
prunus_persica	Prupe.5G134300_v2.0.a1 Prupe.5G134300_v2.0.a1 Prupe.5G134300_v2.0.a1
pyrus_communis	pycom14g11570
rosa_chinensis	RchiOBHm_Chr4g0431771 RchiOBHm_Chr7g0187761
rosa_laevigata	RLG00000004758 RLG00000006891
rosa_multiflora	Rmu_sc0001459.1_g000019 Rmu_sc0004483.1_g000029
rosa_roxburghii	Rroxscaffold_3G00267100 Rroxscaffold_5G00373170 Rroxscaffold_5G00373180
rosa_rugosa	Rorug04G0252300 Rorug06G0484000.1
rosa_samantha	Rh4AG308700 Rh4BG316500 Rh4CG331900 Rh4DG311400 Rh7AG089100 Rh7BG090900 Rh7CG089900 Rh7DG091500
rosa_wichuraiana	Rw0G003020 Rw4G026780 Rw7G007690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	4
AclWI	GGATC	2 cut(s)	252, 265
AcoI	YGGCCR	2 cut(s)	155, 323
AgsI	TTSAA	1 cut(s)	209
AluBI	AGCT	2 cut(s)	10, 74
AluI	AGCT	2 cut(s)	10, 74
Alw21I	GWGCWC	1 cut(s)	223
AlwI	GGATC	2 cut(s)	252, 265
AoxI	GGCC	2 cut(s)	155, 323
ApeKI	GCWGC	1 cut(s)	247
AsuHPI	GGTGA	1 cut(s)	85
BalI	TGGCCA	2 cut(s)	157, 325
BamHI	GGATCC	1 cut(s)	257
Bbv12I	GWGCWC	1 cut(s)	223
BbvI	GCAGC	1 cut(s)	234
BccI	CCATC	2 cut(s)	159, 166
BceAI	ACGGC	2 cut(s)	117, 208
BfaI	CTAG	1 cut(s)	215
BisI	GCNGC	1 cut(s)	248
BlsI	GCNGC	1 cut(s)	249
BmiI	GGNNCC	1 cut(s)	259
BsaJI	CCNNGG	1 cut(s)	326
Bse1I	ACTGG	1 cut(s)	303
Bse3DI	GCAATG	1 cut(s)	275
BseDI	CCNNGG	1 cut(s)	326
BseGI	GGATG	1 cut(s)	170
BseMI	GCAATG	1 cut(s)	275
BseNI	ACTGG	1 cut(s)	303
BseXI	GCAGC	1 cut(s)	234
BshFI	GGCC	2 cut(s)	157, 325
BsiHKAI	GWGCWC	1 cut(s)	223
BsnI	GGCC	2 cut(s)	157, 325
Bsp1286I	GDGCHC	1 cut(s)	223
Bsp143I	GATC	2 cut(s)	257, 305
Bsp19I	CCATGG	1 cut(s)	326
BspACI	CCGC	1 cut(s)	4
BspANI	GGCC	2 cut(s)	157, 325
BspHI	TCATGA	1 cut(s)	42
BspLI	GGNNCC	1 cut(s)	259
BspPI	GGATC	2 cut(s)	252, 265
BsrDI	GCAATG	1 cut(s)	275
BsrI	ACTGG	1 cut(s)	303
BssECI	CCNNGG	1 cut(s)	326
BssMI	GATC	2 cut(s)	257, 305
BssT1I	CCWWGG	1 cut(s)	326
BstC8I	GCNNGC	1 cut(s)	252
BstDSI	CCRYGG	1 cut(s)	326
BstF5I	GGATG	1 cut(s)	170
BstKTI	GATC	2 cut(s)	260, 308
BstMBI	GATC	2 cut(s)	257, 305
BstV1I	GCAGC	1 cut(s)	234
BstX2I	RGATCY	1 cut(s)	257
BstXI	CCANNNNNNTGG	1 cut(s)	165
BstYI	RGATCY	1 cut(s)	257
BsuRI	GGCC	2 cut(s)	157, 325
BtgI	CCRYGG	1 cut(s)	326
BtsCI	GGATG	1 cut(s)	170
BtsIMutI	CAGTG	1 cut(s)	296
Cac8I	GCNNGC	1 cut(s)	252
CciI	TCATGA	1 cut(s)	42
CviAII	CATG	7 cut(s)	43, 315, 321, 327, 336, 342, 348
CviJI	RGCY	9 cut(s)	10, 74, 81, 104, 157, 195, 247, 274, 325
CviKI_1	RGCY	9 cut(s)	10, 74, 81, 104, 157, 195, 247, 274, 325
DpnI	GATC	2 cut(s)	259, 307
DpnII	GATC	2 cut(s)	257, 305
EaeI	YGGCCR	2 cut(s)	155, 323
Eco130I	CCWWGG	1 cut(s)	326
EcoT14I	CCWWGG	1 cut(s)	326
ErhI	CCWWGG	1 cut(s)	326
FaeI	CATG	7 cut(s)	46, 318, 324, 330, 339, 345, 351
FaiI	YATR	8 cut(s)	44, 269, 316, 322, 328, 337, 343, 349
FatI	CATG	7 cut(s)	42, 314, 320, 326, 335, 341, 347
Fnu4HI	GCNGC	1 cut(s)	248
FokI	GGATG	1 cut(s)	177
Fsp4HI	GCNGC	1 cut(s)	248
FspBI	CTAG	1 cut(s)	215
GluI	GCNGC	1 cut(s)	248
HaeIII	GGCC	2 cut(s)	157, 325
Hin1II	CATG	7 cut(s)	46, 318, 324, 330, 339, 345, 351
HindIII	AAGCTT	1 cut(s)	8
HphI	GGTGA	1 cut(s)	85
Hpy188I	TCNGA	2 cut(s)	118, 237
Hpy188III	TCNNGA	3 cut(s)	43, 162, 202
HpyCH4V	TGCA	2 cut(s)	134, 280
Hsp92II	CATG	7 cut(s)	46, 318, 324, 330, 339, 345, 351
Kzo9I	GATC	2 cut(s)	257, 305
LpnPI	CCDG	5 cut(s)	139, 147, 215, 240, 284
Lsp1109I	GCAGC	1 cut(s)	234
MaeI	CTAG	1 cut(s)	215
MalI	GATC	2 cut(s)	259, 307
MboI	GATC	2 cut(s)	257, 305
MboII	GAAGA	1 cut(s)	190
MflI	RGATCY	1 cut(s)	257
MhlI	GDGCHC	1 cut(s)	223
MlsI	TGGCCA	2 cut(s)	157, 325
MluCI	AATT	2 cut(s)	87, 135
MluNI	TGGCCA	2 cut(s)	157, 325
MnlI	CCTC	3 cut(s)	38, 46, 163
Mox20I	TGGCCA	2 cut(s)	157, 325
MscI	TGGCCA	2 cut(s)	157, 325
MslI	CAYNNNNRTG	8 cut(s)	41, 163, 236, 313, 319, 325, 340, 346
Msp20I	TGGCCA	2 cut(s)	157, 325
NcoI	CCATGG	1 cut(s)	326
NdeII	GATC	2 cut(s)	257, 305
NlaIII	CATG	7 cut(s)	46, 318, 324, 330, 339, 345, 351
NlaIV	GGNNCC	1 cut(s)	259
PagI	TCATGA	1 cut(s)	42
PkrI	GCNGC	1 cut(s)	249
PspN4I	GGNNCC	1 cut(s)	259
PsuI	RGATCY	1 cut(s)	257
RseI	CAYNNNNRTG	8 cut(s)	41, 163, 236, 313, 319, 325, 340, 346
SatI	GCNGC	1 cut(s)	248
Sau3AI	GATC	2 cut(s)	257, 305
SduI	GDGCHC	1 cut(s)	223
SetI	ASST	4 cut(s)	12, 57, 76, 220
SmiMI	CAYNNNNRTG	8 cut(s)	41, 163, 236, 313, 319, 325, 340, 346
Sse9I	AATT	2 cut(s)	87, 135
SsiI	CCGC	1 cut(s)	4
SspMI	CTAG	1 cut(s)	215
StyI	CCWWGG	1 cut(s)	326
TasI	AATT	2 cut(s)	87, 135
TscAI	CASTG	1 cut(s)	303
TseI	GCWGC	1 cut(s)	247
TspDTI	ATGAA	2 cut(s)	31, 284
TspGWI	ACGGA	1 cut(s)	325
TspRI	CASTG	1 cut(s)	303
XcmI	CCANNNNNNNNNTGG	1 cut(s)	333
XspI	CTAG	1 cut(s)	215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.