Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0003515

Physical Location & Seq

Genomic Coordinates Forward (+)

62055195 .. 62056283

Gene Structure 1089 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG308700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1089 bp

ATGGAAGTAGGGAATCATTCCGGGTCTGCAAATACTACAGAAATTAAAATGGTTGAAGATCGGGAACGAGATCCGGAGACAAACAGGGTGGTGTCACATAGTTGTGGGGAAGGACGATGTCAAGCGTTAACTTCCAACTATGGAGAGGCATCCAAGAGAAACCTGATGACAGCACTCGTAGCCTCAGTCATTTTCATGACAGTGGAAATTGTTGTAGGCATCAAAGCCAATAGTTTGGCAATTCGGTCCGATGCGCTACATGTCTTAACAGATGTTGTGGCATTTGCAATTTCTGTGTTCTCAGTTTGGGCATCGGGGTGGAAGGCAACTCCAGGCAAGACTTATGGCTTCTTAAGGATTGAAACCCTAGGTGCTCTCGTTTCAATCCTTATAATATGGCTCACTGCTGGTATTCTAATTTATGAAGCCATTGAGAGACTTAAGAATGGCAACCCTGGCGAAGTTAAGGGCTCTCTCATGTTTATTGTTTCCACCGTGGGTTTAGTGCTTAACATCGCCATGGCGGTCTTGTTGGGTCATGATCACAACCATGGCAACAGCCATGGTGATCATCATCATAACGAGGATCATGATGATGATGATCATACAGATAACAATGGGACACATCACAATCGTCGCAATATGAACCTCCATGGGGCATATCTTCATGTACTTGGGGATATCATTCAGAGCATTGGAGTGATGGTCACCGCAGTGGTCATATGGGCTAAGCCTGAATGGAAGATCGCTGATCTCATATGCACACTAGTATTTTCAATAGTTGTGCTGGCTACAACCATCGGCATGCTGCGGAAAATTGTTGGGGTTCTTATGGAGAGCACTCCAAGAGGGATCGACACCTTAAAGCTTGAGAAAGCTTTGTGTGAGCTGGATGCAGTAGTTGCCTGTCATGAACTGCACATATGGTGCATAACTCCTGGGAAGAATTTACTAACTTGCCATCTAATTGTCAACCAAGAAGCTAATTCTTATAGGGTGTTAGAAAATGCTATAAATTATATCAAAACAGAGTACAATATAACTCATGTAACAATTCAAATTGAGCCTCAGCAGCTAGCATCTCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005385 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0022857 GO:0022890 GO:0030001 GO:0032879 GO:0034220 GO:0042592 GO:0046873 GO:0046915 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051179 GO:0051234 GO:0055085 GO:0061088 GO:0065007 GO:0065008 GO:0070838 GO:0071577 GO:0072509 GO:0072511 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux

Protein Analysis

362

Amino Acids

39.75

Weight (kDa)

5.96

Isoelectric Point (pI)

25.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	56 - 278	8.9e-42	Cation efflux transmembrane domain
ZT_dimer	PF16916	283 - 356	1.5e-12	Cation efflux protein, cytoplasmic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003515)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58810 AT3G58810
fragaria_vesca	FvH4_5g10450 FvH4_5g10450 FvH4_5g10450
malus_domestica	MD06G1123800.v1.1 MD13G1032200.v1.1 MD14G1140000.v1.1 MD14G1140800.v1.1
prunus_persica	Prupe.5G134300_v2.0.a1 Prupe.5G134300_v2.0.a1 Prupe.5G134300_v2.0.a1
pyrus_communis	pycom14g11570
rosa_chinensis	RchiOBHm_Chr4g0431771 RchiOBHm_Chr7g0187761
rosa_laevigata	RLG00000004758 RLG00000006891
rosa_multiflora	Rmu_sc0001459.1_g000019 Rmu_sc0004483.1_g000029
rosa_roxburghii	Rroxscaffold_3G00267100 Rroxscaffold_5G00373170 Rroxscaffold_5G00373180
rosa_rugosa	Rorug04G0252300 Rorug06G0484000.1
rosa_samantha	Rh4AG308700 Rh4BG316500 Rh4CG331900 Rh4DG311400 Rh7AG089100 Rh7BG090900 Rh7CG089900 Rh7DG091500
rosa_wichuraiana	Rw0G003020 Rw4G026780 Rw7G007690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	392
AccIII	TCCGGA	1 cut(s)	73
AciI	CCGC	3 cut(s)	524, 711, 811
AclWI	GGATC	3 cut(s)	65, 594, 860
AcsI	RAATTY	1 cut(s)	946
AfaI	GTAC	2 cut(s)	672, 1034
AfiI	CCNNNNNNNGG	1 cut(s)	655
AflII	CTTAAG	2 cut(s)	352, 440
AflIII	ACRYGT	1 cut(s)	259
AgsI	TTSAA	5 cut(s)	56, 362, 384, 777, 1058
AhlI	ACTAGT	1 cut(s)	766
AjnI	CCWGG	3 cut(s)	331, 454, 937
AleI	CACNNNNGTG	1 cut(s)	713
AluBI	AGCT	5 cut(s)	868, 878, 889, 983, 1075
AluI	AGCT	5 cut(s)	868, 878, 889, 983, 1075
Alw21I	GWGCWC	2 cut(s)	376, 842
Alw26I	GTCTC	2 cut(s)	71, 430
AlwI	GGATC	3 cut(s)	65, 594, 860
Aor13HI	TCCGGA	1 cut(s)	73
ApeKI	GCWGC	2 cut(s)	808, 1072
ApoI	RAATTY	1 cut(s)	946
AspA2I	CCTAGG	1 cut(s)	367
AspLEI	GCGC	1 cut(s)	256
AspS9I	GGNCC	1 cut(s)	246
AsuC2I	CCSGG	1 cut(s)	22
AsuHPI	GGTGA	2 cut(s)	578, 700
AsuNHI	GCTAGC	1 cut(s)	1075
AvaII	GGWCC	1 cut(s)	246
AvrII	CCTAGG	1 cut(s)	367
BanII	GRGCYC	1 cut(s)	473
Bbv12I	GWGCWC	2 cut(s)	376, 842
BbvCI	CCTCAGC	1 cut(s)	1068
BbvI	GCAGC	2 cut(s)	795, 1084
BccI	CCATC	3 cut(s)	697, 806, 969
BciT130I	CCWGG	3 cut(s)	333, 456, 939
BclI	TGATCA	3 cut(s)	541, 568, 601
BcnI	CCSGG	1 cut(s)	22
BcoDI	GTCTC	2 cut(s)	71, 430
BcuI	ACTAGT	1 cut(s)	766
BfaI	CTAG	3 cut(s)	368, 767, 1076
BfmI	CTRYAG	1 cut(s)	36
BfrI	CTTAAG	2 cut(s)	352, 440
BisI	GCNGC	2 cut(s)	809, 1073
BlnI	CCTAGG	1 cut(s)	367
BlpI	GCTNAGC	1 cut(s)	729
BlsI	GCNGC	2 cut(s)	810, 1074
Bme1390I	CCNGG	4 cut(s)	22, 333, 456, 939
Bme18I	GGWCC	1 cut(s)	246
BmgT120I	GGNCC	1 cut(s)	246
BmrFI	CCNGG	4 cut(s)	22, 333, 456, 939
BmsI	GCATC	5 cut(s)	158, 228, 241, 320, 883
BmtI	GCTAGC	1 cut(s)	1079
BpmI	CTGGAG	1 cut(s)	315
Bpu10I	CCTNAGC	1 cut(s)	1068
Bpu1102I	GCTNAGC	1 cut(s)	729
BpuEI	CTTGAG	1 cut(s)	890
BpuMI	CCSGG	1 cut(s)	22
BsaBI	GATNNNNATC	2 cut(s)	573, 600
BsaJI	CCNNGG	8 cut(s)	367, 454, 495, 519, 550, 562, 652, 938
BsaWI	WCCGGW	1 cut(s)	73
BsaXI	ACNNNNNCTCC	2 cut(s)	690, 720
Bsc4I	CCNNNNNNNGG	1 cut(s)	655
Bse8I	GATNNNNATC	2 cut(s)	573, 600
BseAI	TCCGGA	1 cut(s)	73
BseBI	CCWGG	3 cut(s)	333, 456, 939
BseDI	CCNNGG	8 cut(s)	367, 454, 495, 519, 550, 562, 652, 938
BseGI	GGATG	2 cut(s)	149, 898
BseJI	GATNNNNATC	2 cut(s)	573, 600
BseLI	CCNNNNNNNGG	1 cut(s)	655
BseMII	CTCAG	3 cut(s)	198, 315, 1082
BseXI	GCAGC	2 cut(s)	795, 1084
BsgI	GTGCAG	1 cut(s)	902
BsiHKAI	GWGCWC	2 cut(s)	376, 842
BsiSI	CCGG	2 cut(s)	21, 74
BslFI	GGGAC	1 cut(s)	634
BslI	CCNNNNNNNGG	1 cut(s)	655
BsmAI	GTCTC	2 cut(s)	71, 430
BsmFI	GGGAC	1 cut(s)	634
Bsp1286I	GDGCHC	3 cut(s)	376, 473, 842
Bsp13I	TCCGGA	1 cut(s)	73
Bsp143I	GATC	9 cut(s)	58, 70, 541, 568, 586, 601, 744, 751, 852
Bsp1720I	GCTNAGC	1 cut(s)	729
Bsp19I	CCATGG	4 cut(s)	519, 550, 562, 652
BspACI	CCGC	3 cut(s)	524, 711, 811
BspCNI	CTCAG	3 cut(s)	197, 314, 1081
BspEI	TCCGGA	1 cut(s)	73
BspHI	TCATGA	4 cut(s)	195, 538, 589, 910
BspOI	GCTAGC	1 cut(s)	1079
BspPI	GGATC	3 cut(s)	65, 594, 860
BspTI	CTTAAG	2 cut(s)	352, 440
BssECI	CCNNGG	8 cut(s)	367, 454, 495, 519, 550, 562, 652, 938
BssMI	GATC	9 cut(s)	58, 70, 541, 568, 586, 601, 744, 751, 852
BssT1I	CCWWGG	5 cut(s)	367, 519, 550, 562, 652
Bst2UI	CCWGG	3 cut(s)	333, 456, 939
Bst4CI	ACNGT	2 cut(s)	202, 496
BstAFI	CTTAAG	2 cut(s)	352, 440
BstAPI	GCANNNNNTGC	1 cut(s)	902
BstC8I	GCNNGC	3 cut(s)	789, 806, 1077
BstDEI	CTNAG	4 cut(s)	184, 301, 729, 1068
BstDSI	CCRYGG	5 cut(s)	495, 519, 550, 562, 652
BstEII	GGTNACC	1 cut(s)	706
BstF5I	GGATG	2 cut(s)	149, 898
BstHHI	GCGC	1 cut(s)	256
BstKTI	GATC	9 cut(s)	61, 73, 544, 571, 589, 604, 747, 754, 855
BstMAI	GTCTC	2 cut(s)	71, 430
BstMBI	GATC	9 cut(s)	58, 70, 541, 568, 586, 601, 744, 751, 852
BstMWI	GCNNNNNNNGC	4 cut(s)	179, 456, 902, 1072
BstNI	CCWGG	3 cut(s)	333, 456, 939
BstNSI	RCATGY	2 cut(s)	263, 808
BstPI	GGTNACC	1 cut(s)	706
BstSCI	CCNGG	4 cut(s)	20, 331, 454, 937
BstSFI	CTRYAG	1 cut(s)	36
BstV1I	GCAGC	2 cut(s)	795, 1084
BstX2I	RGATCY	1 cut(s)	70
BstXI	CCANNNNNNTGG	1 cut(s)	235
BstYI	RGATCY	1 cut(s)	70
BtgI	CCRYGG	5 cut(s)	495, 519, 550, 562, 652
BtgZI	GCGATG	1 cut(s)	499
BtsCI	GGATG	2 cut(s)	149, 898
BtsI	GCAGTG	2 cut(s)	402, 720
BtsIMutI	CAGTG	3 cut(s)	207, 402, 720
Cac8I	GCNNGC	3 cut(s)	789, 806, 1077
CciI	TCATGA	4 cut(s)	195, 538, 589, 910
CfoI	GCGC	1 cut(s)	256
Cfr13I	GGNCC	1 cut(s)	246
CpoI	CGGWCCG	1 cut(s)	246
Csp6I	GTAC	2 cut(s)	671, 1033
CspCI	CAANNNNNGTGG	2 cut(s)	69, 104
CspI	CGGWCCG	1 cut(s)	246
CviQI	GTAC	2 cut(s)	671, 1033
DdeI	CTNAG	4 cut(s)	184, 301, 729, 1068
DpnI	GATC	9 cut(s)	60, 72, 543, 570, 588, 603, 746, 753, 854
DpnII	GATC	9 cut(s)	58, 70, 541, 568, 586, 601, 744, 751, 852
Eco130I	CCWWGG	5 cut(s)	367, 519, 550, 562, 652
Eco24I	GRGCYC	1 cut(s)	473
Eco32I	GATATC	1 cut(s)	682
Eco47I	GGWCC	1 cut(s)	246
Eco91I	GGTNACC	1 cut(s)	706
EcoO65I	GGTNACC	1 cut(s)	706
EcoRII	CCWGG	3 cut(s)	331, 454, 937
EcoRV	GATATC	1 cut(s)	682
EcoT14I	CCWWGG	5 cut(s)	367, 519, 550, 562, 652
EcoT38I	GRGCYC	1 cut(s)	473
ErhI	CCWWGG	5 cut(s)	367, 519, 550, 562, 652
FaqI	GGGAC	1 cut(s)	634
FauNDI	CATATG	3 cut(s)	722, 758, 923
FbaI	TGATCA	3 cut(s)	541, 568, 601
Fnu4HI	GCNGC	2 cut(s)	809, 1073
FokI	GGATG	2 cut(s)	136, 905
FriOI	GRGCYC	1 cut(s)	473
Fsp4HI	GCNGC	2 cut(s)	809, 1073
FspBI	CTAG	3 cut(s)	368, 767, 1076
GlaI	GCGC	1 cut(s)	255
GluI	GCNGC	2 cut(s)	809, 1073
GsuI	CTGGAG	1 cut(s)	315
HapII	CCGG	2 cut(s)	21, 74
HhaI	GCGC	1 cut(s)	256
Hin6I	GCGC	1 cut(s)	254
HinP1I	GCGC	1 cut(s)	254
HincII	GTYRAC	2 cut(s)	129, 973
HindII	GTYRAC	2 cut(s)	129, 973
HindIII	AAGCTT	2 cut(s)	866, 876
HinfI	GANTC	1 cut(s)	13
HpaI	GTTAAC	1 cut(s)	129
HpaII	CCGG	2 cut(s)	21, 74
HphI	GGTGA	2 cut(s)	578, 700
Hpy166II	GTNNAC	2 cut(s)	129, 973
Hpy188I	TCNGA	2 cut(s)	250, 690
Hpy188III	TCNNGA	6 cut(s)	62, 74, 196, 539, 590, 911
Hpy8I	GTNNAC	2 cut(s)	129, 973
Hpy99I	CGWCG	1 cut(s)	639
HpyAV	CCTTC	2 cut(s)	104, 316
HpyCH4III	ACNGT	2 cut(s)	202, 496
HpyCH4V	TGCA	6 cut(s)	29, 287, 762, 896, 919, 930
HpyF10VI	GCNNNNNNNGC	4 cut(s)	179, 456, 902, 1072
HpyF3I	CTNAG	4 cut(s)	184, 301, 729, 1068
HspAI	GCGC	1 cut(s)	254
Kpn2I	TCCGGA	1 cut(s)	73
Ksp22I	TGATCA	3 cut(s)	541, 568, 601
KspAI	GTTAAC	1 cut(s)	129
Kzo9I	GATC	9 cut(s)	58, 70, 541, 568, 586, 601, 744, 751, 852
Lsp1109I	GCAGC	2 cut(s)	795, 1084
LweI	GCATC	5 cut(s)	158, 228, 241, 320, 883
MaeI	CTAG	3 cut(s)	368, 767, 1076
MaeIII	GTNAC	3 cut(s)	93, 706, 1048
MalI	GATC	9 cut(s)	60, 72, 543, 570, 588, 603, 746, 753, 854
MboI	GATC	9 cut(s)	58, 70, 541, 568, 586, 601, 744, 751, 852
MboII	GAAGA	4 cut(s)	68, 656, 754, 955
MflI	RGATCY	1 cut(s)	70
MhlI	GDGCHC	3 cut(s)	376, 473, 842
MmeI	TCCRAC	1 cut(s)	159
MnlI	CCTC	6 cut(s)	139, 193, 577, 659, 842, 1077
MroI	TCCGGA	1 cut(s)	73
MseI	TTAA	8 cut(s)	45, 128, 266, 353, 441, 465, 510, 863
MspCI	CTTAAG	2 cut(s)	352, 440
MspI	CCGG	2 cut(s)	21, 74
MspR9I	CCNGG	4 cut(s)	22, 333, 456, 939
MvaI	CCWGG	3 cut(s)	333, 456, 939
MwoI	GCNNNNNNNGC	4 cut(s)	179, 456, 902, 1072
NciI	CCSGG	1 cut(s)	22
NcoI	CCATGG	4 cut(s)	519, 550, 562, 652
NdeI	CATATG	3 cut(s)	722, 758, 923
NdeII	GATC	9 cut(s)	58, 70, 541, 568, 586, 601, 744, 751, 852
NheI	GCTAGC	1 cut(s)	1075
NmuCI	GTSAC	2 cut(s)	93, 706
NspI	RCATGY	2 cut(s)	263, 808
OliI	CACNNNNGTG	1 cut(s)	713
PaeI	GCATGC	1 cut(s)	808
PagI	TCATGA	4 cut(s)	195, 538, 589, 910
PciI	ACATGT	1 cut(s)	259
PfeI	GAWTC	1 cut(s)	13
PflFI	GACNNNGTC	1 cut(s)	117
PkrI	GCNGC	2 cut(s)	810, 1074
PscI	ACATGT	1 cut(s)	259
PsiI	TTATAA	1 cut(s)	392
Psp6I	CCWGG	3 cut(s)	331, 454, 937
PspEI	GGTNACC	1 cut(s)	706
PspGI	CCWGG	3 cut(s)	331, 454, 937
PspPI	GGNCC	1 cut(s)	246
PsuI	RGATCY	1 cut(s)	70
PsyI	GACNNNGTC	1 cut(s)	117
RsaI	GTAC	2 cut(s)	672, 1034
RsaNI	GTAC	2 cut(s)	671, 1033
Rsr2I	CGGWCCG	1 cut(s)	246
RsrII	CGGWCCG	1 cut(s)	246
SaqAI	TTAA	8 cut(s)	45, 128, 266, 353, 441, 465, 510, 863
SatI	GCNGC	2 cut(s)	809, 1073
Sau3AI	GATC	9 cut(s)	58, 70, 541, 568, 586, 601, 744, 751, 852
Sau96I	GGNCC	1 cut(s)	246
ScrFI	CCNGG	4 cut(s)	22, 333, 456, 939
SduI	GDGCHC	3 cut(s)	376, 473, 842
SetI	ASST	9 cut(s)	165, 373, 651, 863, 870, 880, 891, 985, 1077
SfaNI	GCATC	5 cut(s)	158, 228, 241, 320, 883
SfcI	CTRYAG	1 cut(s)	36
SinI	GGWCC	1 cut(s)	246
SmlI	CTYRAG	3 cut(s)	352, 440, 869
SmoI	CTYRAG	3 cut(s)	352, 440, 869
SpeI	ACTAGT	1 cut(s)	766
SphI	GCATGC	1 cut(s)	808
SsiI	CCGC	3 cut(s)	524, 711, 811
SspMI	CTAG	3 cut(s)	368, 767, 1076
StyD4I	CCNGG	4 cut(s)	20, 331, 454, 937
StyI	CCWWGG	5 cut(s)	367, 519, 550, 562, 652
TaaI	ACNGT	2 cut(s)	202, 496
TaqI	TCGA	1 cut(s)	855
TaqII	GACCGA	1 cut(s)	234
TatI	WGTACW	2 cut(s)	670, 1032
TfiI	GAWTC	1 cut(s)	13
Tru1I	TTAA	8 cut(s)	45, 128, 266, 353, 441, 465, 510, 863
Tru9I	TTAA	8 cut(s)	45, 128, 266, 353, 441, 465, 510, 863
TscAI	CASTG	3 cut(s)	207, 409, 720
TseFI	GTSAC	2 cut(s)	93, 706
TseI	GCWGC	2 cut(s)	808, 1072
Tsp45I	GTSAC	2 cut(s)	93, 706
TspDTI	ATGAA	5 cut(s)	184, 438, 656, 659, 927
TspRI	CASTG	3 cut(s)	207, 409, 720
Tth111I	GACNNNGTC	1 cut(s)	117
Vha464I	CTTAAG	2 cut(s)	352, 440
VpaK11BI	GGWCC	1 cut(s)	246
XapI	RAATTY	1 cut(s)	946
XceI	RCATGY	2 cut(s)	263, 808
XmaJI	CCTAGG	1 cut(s)	367
XspI	CTAG	3 cut(s)	368, 767, 1076

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.