Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003515

Physical Location & Seq

Genomic Coordinates Forward (+)

23036992 .. 23039776

Gene Structure 2785 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1140000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1347 bp

ATGTATGGAATGGAAGCTCAAAATTCTGGGCATTCGCATGTAGTTGAAGTAGGTGGAGATGTGCCAGCTACAGATACGAGCTTGTGTGGGACCAAGATTTGTGGGGATGCACCATGCGGATTTTCAGATGCTAAAACTAGTTACAAAGATGCACAGGAACGGTCAGCATCCATGCGGAAGCTTCTGATAGCAGTTGTCCTCTGTGTCGTTTTCATGAGTGTGGAGGTTGTTGGGGGTATCAAAGCCAACAGTCTTGCGATTCTCACTGATGCGGCTCATTTGTTGTCAGATGTTGCCGCATTTGCAATTTCTTTGTTCTCACTTTGGGCATCAGGATGGGAGGCAACTCCTCGTCAGTCGTATGGCTTCTTCCGGATTGAAATACTAGGTGCTCTTGTTTCCATTCAGATGATTTGGCTGCTTGCTGGGATCCTTGTTTATGAAGCCATTGCAAGACTTATCCATGGCACTGGTGAAGTTGAGGGCTTTCTCATGTTTCTTGTTTCTGCGTTTGGTTTACTGGTTAATATCGCCATGGCACTCTTATTGGGTCATGATCATGGGCACGATCATGGACATGGACATGGACATGGCCATGGTGGGCACGGACATGGACATGGCCATGGTGGGCACGGACATGGACATGGCCATGGTGGGCATGGTCATGGTCATGGAGATGGTGATCATAATCATGGTCATGGAGATGGTGATCATAATCATAGTCATGAGGATCATGATGAGACCCATAACCATGGGATTACTGTGACAACACATCACTCTCATCATCATGAGGTGCACTCGAATCATGACGACAAGCACCATCATACCGATGTAATTAACAGCACAGAACCTCTGCTCAATCATAGCTCTGAAGGTGAAAAGAAACCAGAAGGTAAAAAGAAGAAGCAACGAAACATCAATGTGCAGGGGGCTTATCTTCATGTACTTGGCGATTCCATTCAGAGTATTGGAGTGATGATTGGTGGAGGGCTTATCTGGTACAAGCCAGAGTGGAAGATTATTGATCTGATATGTACTCTTGTATTTTCAATAATCGTGCTGTCGACAACAATTCGGATGTTGAGGAACATTTTGGAGGTTTTGATGGAGAGCACTCCCAGGGAGATTGATGCCACAAAGATTGAGAAAGGTCTCTGCGAGATGGATGAGGTAGTTGCCATTCACGAGCTGCACATATGGGCGATAACTGTTGGGAAGGTGTTAATGGCTTGTCATGTTATCGTTAAGCCGGATGCTAATGCCGATATGGTGCTGGAAAAGGTTGTAGACTACATCAAGAGAGAATATAATATCAGTCATGTAACGATTCAGATAGAACGTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005385 GO:0006810 GO:0006811 GO:0006812 GO:0006829 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0022857 GO:0022890 GO:0030001 GO:0032879 GO:0034220 GO:0042592 GO:0046873 GO:0046915 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0051179 GO:0051234 GO:0055085 GO:0061088 GO:0065007 GO:0065008 GO:0070838 GO:0071577 GO:0072509 GO:0072511 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_efflux

Protein Analysis

449

Amino Acids

48.77

Weight (kDa)

6.11

Isoelectric Point (pI)

26.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_efflux	PF01545	61 - 369	1.8e-36	Cation efflux transmembrane domain
ZT_dimer	PF16916	373 - 447	3.6e-14	Cation efflux protein, cytoplasmic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003515)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G58810 AT3G58810
fragaria_vesca	FvH4_5g10450 FvH4_5g10450 FvH4_5g10450
malus_domestica	MD06G1123800.v1.1 MD13G1032200.v1.1 MD14G1140000.v1.1 MD14G1140800.v1.1
prunus_persica	Prupe.5G134300_v2.0.a1 Prupe.5G134300_v2.0.a1 Prupe.5G134300_v2.0.a1
pyrus_communis	pycom14g11570
rosa_chinensis	RchiOBHm_Chr4g0431771 RchiOBHm_Chr7g0187761
rosa_laevigata	RLG00000004758 RLG00000006891
rosa_multiflora	Rmu_sc0001459.1_g000019 Rmu_sc0004483.1_g000029
rosa_roxburghii	Rroxscaffold_3G00267100 Rroxscaffold_5G00373170 Rroxscaffold_5G00373180
rosa_rugosa	Rorug04G0252300 Rorug06G0484000.1
rosa_samantha	Rh4AG308700 Rh4BG316500 Rh4CG331900 Rh4DG311400 Rh7AG089100 Rh7BG090900 Rh7CG089900 Rh7DG091500
rosa_wichuraiana	Rw0G003020 Rw4G026780 Rw7G007690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	1064, 1287
AccIII	TCCGGA	1 cut(s)	372
AciI	CCGC	4 cut(s)	117, 175, 272, 297
AclWI	GGATC	3 cut(s)	424, 437, 738
AcoI	YGGCCR	3 cut(s)	592, 619, 646
AcsI	RAATTY	1 cut(s)	22
AcuI	CTGAAG	1 cut(s)	891
AfaI	GTAC	3 cut(s)	945, 1001, 1036
AgsI	TTSAA	3 cut(s)	47, 380, 1050
AhlI	ACTAGT	1 cut(s)	137
AjnI	CCWGG	1 cut(s)	1118
AluBI	AGCT	6 cut(s)	17, 68, 81, 181, 867, 1189
AluI	AGCT	6 cut(s)	17, 68, 81, 181, 867, 1189
Alw21I	GWGCWC	3 cut(s)	394, 798, 1115
Alw26I	GTCTC	2 cut(s)	734, 1157
Alw44I	GTGCAC	1 cut(s)	794
AlwI	GGATC	3 cut(s)	424, 437, 738
Aor13HI	TCCGGA	1 cut(s)	372
AoxI	GGCC	3 cut(s)	592, 619, 646
ApaLI	GTGCAC	1 cut(s)	794
ApeKI	GCWGC	2 cut(s)	418, 1189
ApoI	RAATTY	1 cut(s)	22
AspS9I	GGNCC	1 cut(s)	90
AsuHPI	GGTGA	4 cut(s)	485, 692, 719, 887
AvaII	GGWCC	1 cut(s)	90
BaeGI	GKGCMC	4 cut(s)	567, 606, 633, 798
BalI	TGGCCA	3 cut(s)	594, 621, 648
BamHI	GGATCC	1 cut(s)	429
BarI	GAAGNNNNNNTAC	2 cut(s)	353, 385
BauI	CACGAG	1 cut(s)	1184
Bbv12I	GWGCWC	3 cut(s)	394, 798, 1115
BbvI	GCAGC	2 cut(s)	405, 1176
BccI	CCATC	6 cut(s)	330, 671, 698, 828, 1099, 1156
BciT130I	CCWGG	1 cut(s)	1120
BclI	TGATCA	3 cut(s)	556, 682, 709
BcoDI	GTCTC	2 cut(s)	734, 1157
BcuI	ACTAGT	1 cut(s)	137
BfaI	CTAG	2 cut(s)	138, 386
BfmI	CTRYAG	1 cut(s)	69
BisI	GCNGC	4 cut(s)	273, 297, 419, 1190
BlsI	GCNGC	4 cut(s)	274, 298, 420, 1191
Bme1390I	CCNGG	1 cut(s)	1120
Bme18I	GGWCC	1 cut(s)	90
BmgT120I	GGNCC	1 cut(s)	90
BmiI	GGNNCC	2 cut(s)	91, 431
BmrFI	CCNGG	1 cut(s)	1120
BmsI	GCATC	8 cut(s)	97, 118, 139, 176, 259, 338, 1120, 1243
BplI	GAGNNNNNCTC	2 cut(s)	782, 814
BsaBI	GATNNNNATC	4 cut(s)	681, 687, 708, 714
BsaI	GGTCTC	2 cut(s)	734, 1157
BsaJI	CCNNGG	8 cut(s)	463, 534, 595, 622, 649, 751, 1118, 1119
BsaWI	WCCGGW	1 cut(s)	372
Bse1I	ACTGG	2 cut(s)	475, 525
Bse3DI	GCAATG	1 cut(s)	447
Bse8I	GATNNNNATC	4 cut(s)	681, 687, 708, 714
BseAI	TCCGGA	1 cut(s)	372
BseBI	CCWGG	1 cut(s)	1120
BseDI	CCNNGG	8 cut(s)	463, 534, 595, 622, 649, 751, 1118, 1119
BseGI	GGATG	6 cut(s)	112, 167, 341, 1083, 1171, 1258
BseJI	GATNNNNATC	4 cut(s)	681, 687, 708, 714
BseMI	GCAATG	1 cut(s)	447
BseNI	ACTGG	2 cut(s)	475, 525
BseRI	GAGGAG	1 cut(s)	339
BseSI	GKGCMC	4 cut(s)	567, 606, 633, 798
BseXI	GCAGC	2 cut(s)	405, 1176
BseYI	CCCAGC	1 cut(s)	425
BsgI	GTGCAG	2 cut(s)	944, 1175
BshFI	GGCC	3 cut(s)	594, 621, 648
BsiHKAI	GWGCWC	3 cut(s)	394, 798, 1115
BsiSI	CCGG	2 cut(s)	373, 1250
BslFI	GGGAC	1 cut(s)	103
BsmAI	GTCTC	2 cut(s)	734, 1157
BsmFI	GGGAC	1 cut(s)	103
BsmI	GAATGC	1 cut(s)	31
BsnI	GGCC	3 cut(s)	594, 621, 648
Bso31I	GGTCTC	2 cut(s)	734, 1157
Bsp1286I	GDGCHC	6 cut(s)	394, 567, 606, 633, 798, 1115
Bsp13I	TCCGGA	1 cut(s)	372
Bsp143I	GATC	7 cut(s)	429, 556, 568, 682, 709, 730, 1024
Bsp19I	CCATGG	6 cut(s)	463, 534, 595, 622, 649, 751
BspACI	CCGC	4 cut(s)	117, 175, 272, 297
BspANI	GGCC	3 cut(s)	594, 621, 648
BspEI	TCCGGA	1 cut(s)	372
BspHI	TCATGA	6 cut(s)	213, 553, 724, 733, 787, 805
BspLI	GGNNCC	2 cut(s)	91, 431
BspPI	GGATC	3 cut(s)	424, 437, 738
BspTNI	GGTCTC	2 cut(s)	734, 1157
BsrDI	GCAATG	1 cut(s)	447
BsrI	ACTGG	2 cut(s)	475, 525
BssECI	CCNNGG	8 cut(s)	463, 534, 595, 622, 649, 751, 1118, 1119
BssMI	GATC	7 cut(s)	429, 556, 568, 682, 709, 730, 1024
BssSI	CACGAG	1 cut(s)	1184
BssT1I	CCWWGG	6 cut(s)	463, 534, 595, 622, 649, 751
Bst2BI	CACGAG	1 cut(s)	1184
Bst2UI	CCWGG	1 cut(s)	1120
Bst4CI	ACNGT	4 cut(s)	162, 251, 763, 1210
BstC8I	GCNNGC	2 cut(s)	66, 423
BstDSI	CCRYGG	6 cut(s)	463, 534, 595, 622, 649, 751
BstF5I	GGATG	6 cut(s)	112, 167, 341, 1083, 1171, 1258
BstKTI	GATC	7 cut(s)	432, 559, 571, 685, 712, 733, 1027
BstMAI	GTCTC	2 cut(s)	734, 1157
BstMBI	GATC	7 cut(s)	429, 556, 568, 682, 709, 730, 1024
BstMWI	GCNNNNNNNGC	1 cut(s)	302
BstNI	CCWGG	1 cut(s)	1120
BstNSI	RCATGY	1 cut(s)	41
BstSCI	CCNGG	1 cut(s)	1118
BstSFI	CTRYAG	1 cut(s)	69
BstSLI	GKGCMC	4 cut(s)	567, 606, 633, 798
BstV1I	GCAGC	2 cut(s)	405, 1176
BstX2I	RGATCY	1 cut(s)	429
BstXI	CCANNNNNNTGG	2 cut(s)	470, 752
BstYI	RGATCY	1 cut(s)	429
BsuRI	GGCC	3 cut(s)	594, 621, 648
BtgI	CCRYGG	6 cut(s)	463, 534, 595, 622, 649, 751
BtsCI	GGATG	6 cut(s)	112, 167, 341, 1083, 1171, 1258
BtsIMutI	CAGTG	2 cut(s)	264, 468
Cac8I	GCNNGC	2 cut(s)	66, 423
CciI	TCATGA	6 cut(s)	213, 553, 724, 733, 787, 805
Cfr13I	GGNCC	1 cut(s)	90
Csp6I	GTAC	3 cut(s)	944, 1000, 1035
CspCI	CAANNNNNGTGG	4 cut(s)	82, 117, 1123, 1158
CviQI	GTAC	3 cut(s)	944, 1000, 1035
DpnI	GATC	7 cut(s)	431, 558, 570, 684, 711, 732, 1026
DpnII	GATC	7 cut(s)	429, 556, 568, 682, 709, 730, 1024
EaeI	YGGCCR	3 cut(s)	592, 619, 646
Eco130I	CCWWGG	6 cut(s)	463, 534, 595, 622, 649, 751
Eco31I	GGTCTC	2 cut(s)	734, 1157
Eco47I	GGWCC	1 cut(s)	90
Eco57I	CTGAAG	1 cut(s)	891
EcoRII	CCWGG	1 cut(s)	1118
EcoT14I	CCWWGG	6 cut(s)	463, 534, 595, 622, 649, 751
ErhI	CCWWGG	6 cut(s)	463, 534, 595, 622, 649, 751
FaqI	GGGAC	1 cut(s)	103
FauNDI	CATATG	1 cut(s)	1196
FbaI	TGATCA	3 cut(s)	556, 682, 709
FblI	GTMKAC	2 cut(s)	1064, 1287
Fnu4HI	GCNGC	4 cut(s)	273, 297, 419, 1190
FokI	GGATG	6 cut(s)	119, 154, 348, 1090, 1178, 1265
Fsp4HI	GCNGC	4 cut(s)	273, 297, 419, 1190
FspBI	CTAG	2 cut(s)	138, 386
GluI	GCNGC	4 cut(s)	273, 297, 419, 1190
GsaI	CCCAGC	1 cut(s)	429
HaeIII	GGCC	3 cut(s)	594, 621, 648
HapII	CCGG	2 cut(s)	373, 1250
HincII	GTYRAC	1 cut(s)	1065
HindII	GTYRAC	1 cut(s)	1065
HindIII	AAGCTT	1 cut(s)	179
HinfI	GANTC	4 cut(s)	259, 802, 953, 1327
HpaII	CCGG	2 cut(s)	373, 1250
HphI	GGTGA	4 cut(s)	485, 692, 719, 887
Hpy166II	GTNNAC	4 cut(s)	518, 796, 1065, 1288
Hpy188I	TCNGA	9 cut(s)	127, 186, 289, 408, 871, 963, 1029, 1077, 1332
Hpy8I	GTNNAC	4 cut(s)	518, 796, 1065, 1288
HpyAV	CCTTC	3 cut(s)	866, 884, 1210
HpyCH4III	ACNGT	4 cut(s)	162, 251, 763, 1210
HpyCH4IV	ACGT	1 cut(s)	1339
HpyCH4V	TGCA	7 cut(s)	110, 152, 305, 452, 796, 925, 1192
HpyF10VI	GCNNNNNNNGC	1 cut(s)	302
HpySE526I	ACGT	1 cut(s)	1339
Kpn2I	TCCGGA	1 cut(s)	372
Ksp22I	TGATCA	3 cut(s)	556, 682, 709
Kzo9I	GATC	7 cut(s)	429, 556, 568, 682, 709, 730, 1024
Lsp1109I	GCAGC	2 cut(s)	405, 1176
LweI	GCATC	8 cut(s)	97, 118, 139, 176, 259, 338, 1120, 1243
MaeI	CTAG	2 cut(s)	138, 386
MaeII	ACGT	1 cut(s)	1339
MaeIII	GTNAC	3 cut(s)	140, 763, 1321
MalI	GATC	7 cut(s)	431, 558, 570, 684, 711, 732, 1026
MboI	GATC	7 cut(s)	429, 556, 568, 682, 709, 730, 1024
MboII	GAAGA	4 cut(s)	361, 913, 929, 1027
MflI	RGATCY	1 cut(s)	429
MhlI	GDGCHC	6 cut(s)	394, 567, 606, 633, 798, 1115
MlsI	TGGCCA	3 cut(s)	594, 621, 648
MluCI	AATT	4 cut(s)	22, 306, 834, 1071
MluNI	TGGCCA	3 cut(s)	594, 621, 648
Mox20I	TGGCCA	3 cut(s)	594, 621, 648
MroI	TCCGGA	1 cut(s)	372
MscI	TGGCCA	3 cut(s)	594, 621, 648
MseI	TTAA	4 cut(s)	525, 837, 1223, 1245
Msp20I	TGGCCA	3 cut(s)	594, 621, 648
MspI	CCGG	2 cut(s)	373, 1250
MspR9I	CCNGG	1 cut(s)	1120
Mva1269I	GAATGC	1 cut(s)	31
MvaI	CCWGG	1 cut(s)	1120
MwoI	GCNNNNNNNGC	1 cut(s)	302
NcoI	CCATGG	6 cut(s)	463, 534, 595, 622, 649, 751
NdeI	CATATG	1 cut(s)	1196
NdeII	GATC	7 cut(s)	429, 556, 568, 682, 709, 730, 1024
NlaIV	GGNNCC	2 cut(s)	91, 431
NmuCI	GTSAC	1 cut(s)	763
NspI	RCATGY	1 cut(s)	41
PagI	TCATGA	6 cut(s)	213, 553, 724, 733, 787, 805
PasI	CCCWGGG	1 cut(s)	1119
PctI	GAATGC	1 cut(s)	31
PfeI	GAWTC	4 cut(s)	259, 802, 953, 1327
PkrI	GCNGC	4 cut(s)	274, 298, 420, 1191
Psp6I	CCWGG	1 cut(s)	1118
PspFI	CCCAGC	1 cut(s)	425
PspGI	CCWGG	1 cut(s)	1118
PspN4I	GGNNCC	2 cut(s)	91, 431
PspPI	GGNCC	1 cut(s)	90
PsuI	RGATCY	1 cut(s)	429
RsaI	GTAC	3 cut(s)	945, 1001, 1036
RsaNI	GTAC	3 cut(s)	944, 1000, 1035
SalI	GTCGAC	1 cut(s)	1063
SaqAI	TTAA	4 cut(s)	525, 837, 1223, 1245
SatI	GCNGC	4 cut(s)	273, 297, 419, 1190
Sau3AI	GATC	7 cut(s)	429, 556, 568, 682, 709, 730, 1024
Sau96I	GGNCC	1 cut(s)	90
ScrFI	CCNGG	1 cut(s)	1120
SduI	GDGCHC	6 cut(s)	394, 567, 606, 633, 798, 1115
SfaNI	GCATC	8 cut(s)	97, 118, 139, 176, 259, 338, 1120, 1243
SfcI	CTRYAG	1 cut(s)	69
SinI	GGWCC	1 cut(s)	90
SpeI	ACTAGT	1 cut(s)	137
Sse9I	AATT	4 cut(s)	22, 306, 834, 1071
SsiI	CCGC	4 cut(s)	117, 175, 272, 297
SspMI	CTAG	2 cut(s)	138, 386
StyD4I	CCNGG	1 cut(s)	1118
StyI	CCWWGG	6 cut(s)	463, 534, 595, 622, 649, 751
TaaI	ACNGT	4 cut(s)	162, 251, 763, 1210
TaiI	ACGT	1 cut(s)	1342
TaqI	TCGA	2 cut(s)	800, 1064
TasI	AATT	4 cut(s)	22, 306, 834, 1071
TatI	WGTACW	2 cut(s)	943, 1034
TauI	GCSGC	2 cut(s)	275, 299
TfiI	GAWTC	4 cut(s)	259, 802, 953, 1327
Tru1I	TTAA	4 cut(s)	525, 837, 1223, 1245
Tru9I	TTAA	4 cut(s)	525, 837, 1223, 1245
TscAI	CASTG	2 cut(s)	271, 475
TseFI	GTSAC	1 cut(s)	763
TseI	GCWGC	2 cut(s)	418, 1189
Tsp45I	GTSAC	1 cut(s)	763
TspDTI	ATGAA	3 cut(s)	202, 456, 929
TspGWI	ACGGA	2 cut(s)	621, 648
TspRI	CASTG	2 cut(s)	271, 475
VneI	GTGCAC	1 cut(s)	794
VpaK11BI	GGWCC	1 cut(s)	90
XapI	RAATTY	1 cut(s)	22
XceI	RCATGY	1 cut(s)	41
XcmI	CCANNNNNNNNNTGG	1 cut(s)	656
XmiI	GTMKAC	2 cut(s)	1064, 1287
XspI	CTAG	2 cut(s)	138, 386

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.