MD06G1128900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001918
Physical Location & Seq
Forward (+)
27266279 .. 27267779
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1128900.v1.1.491

Sequence Viewer

Length: 720 bp
ATGACCTCCACAAATCCAACCATGGCCATGTCCATGGCTTTTCTTCTCCTCCTCCTCCTCCCGACGCCACTCAATCTGCCGCTGACGACGATGATGACGGCGGTAGAGTCCGCCTCCGACGACCCGGCACCGGCGGCGTGGCCCCACCAGTTCCACTCTGTCCTCTTCATGAACAACAGCAAGGGAGCCCTGCAAGTGGTGGATCTCTGGTACGACTGGCCCAACGGCCGCAACTTCAACATAATCCAAAGCCAGCTGGGGAAGCTCACCTACGACCTCGAATGGGACAATGGCACCTCGTACATCTACACCTTGGACTCCGACAGAGAATGCAACACCATGCATTTTCCGGTTGGCATTCTCCGGCCTGATTGGCTCGACGGCGCTAATTATCTGGGTCAGCAACACGTGGACGGGTTTCTGTGCAATGTGTGGGAGAAGGTTGATTTTATTTGGTACTATGAGGACGTGCTCACCAGGAGACCTGTCCATTGGGTTTTCTATACCGGATATACTGCTCATGTGATGACATTCGAGGTGGGAGCTGTGCTTGAGGATGCCAAGTGGGATGCTCCTGCTCATTGTTTTGGGGAGGAAAAGACTGATTCAGCACAAAGGGGGAGGTCTCCTCCACTTCTACTTGAATCTGTGACGAGTGATTATTCTCATGGCAGGCTGATGAGAGATGAAAGGAGCATTCGAAACATGGATAGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

27.41

Weight (kDa)

4.79

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 127, 293
AciI CCGC 5 cut(s) 80, 101, 111, 134, 229
AclWI GGATC 1 cut(s) 210
AcoI YGGCCR 2 cut(s) 24, 226
AcvI CACGTG 1 cut(s) 409
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 3 cut(s) 212, 302, 458
AfiI CCNNNNNNNGG 3 cut(s) 130, 196, 283
AflIII ACRYGT 1 cut(s) 406
AgsI TTSAA 2 cut(s) 238, 644
AjiI CACGTC 1 cut(s) 469
AjnI CCWGG 1 cut(s) 476
AluBI AGCT 4 cut(s) 256, 265, 545, 714
AluI AGCT 4 cut(s) 256, 265, 545, 714
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 2 cut(s) 475, 630
AlwI GGATC 1 cut(s) 210
AoxI GGCC 5 cut(s) 24, 140, 218, 226, 365
AspLEI GCGC 1 cut(s) 386
AspS9I GGNCC 2 cut(s) 141, 219
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 2 cut(s) 259, 466
AsuII TTCGAA 1 cut(s) 700
BalI TGGCCA 1 cut(s) 26
BanI GGYRCC 2 cut(s) 127, 293
BanII GRGCYC 1 cut(s) 190
BarI GAAGNNNNNNTAC 2 cut(s) 254, 286
BbrPI CACGTG 1 cut(s) 409
Bbv12I GWGCWC 1 cut(s) 474
BceAI ACGGC 3 cut(s) 114, 241, 397
BciT130I CCWGG 1 cut(s) 478
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 2 cut(s) 475, 630
BfoI RGCGCY 1 cut(s) 387
BglI GCCNNNNNGGC 1 cut(s) 373
BisI GCNGC 3 cut(s) 80, 135, 229
BlsI GCNGC 3 cut(s) 81, 136, 230
Bme1390I CCNGG 2 cut(s) 125, 478
BmgBI CACGTC 1 cut(s) 469
BmgT120I GGNCC 2 cut(s) 141, 219
BmiI GGNNCC 4 cut(s) 129, 143, 187, 295
BmrFI CCNGG 2 cut(s) 125, 478
BmsI GCATC 2 cut(s) 547, 559
BplI GAGNNNNNCTC 4 cut(s) 98, 130, 613, 645
Bpu14I TTCGAA 1 cut(s) 700
BpuEI CTTGAG 1 cut(s) 572
BpuMI CCSGG 1 cut(s) 125
BsaAI YACGTR 1 cut(s) 409
BsaHI GRCGYC 1 cut(s) 65
BsaI GGTCTC 2 cut(s) 475, 630
BsaJI CCNNGG 3 cut(s) 21, 33, 312
BsaWI WCCGGW 2 cut(s) 349, 506
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 196, 283
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 2 cut(s) 148, 221
Bse3DI GCAATG 1 cut(s) 433
BseBI CCWGG 1 cut(s) 478
BseDI CCNNGG 3 cut(s) 21, 33, 312
BseGI GGATG 2 cut(s) 562, 574
BseLI CCNNNNNNNGG 3 cut(s) 130, 196, 283
BseMI GCAATG 1 cut(s) 433
BseNI ACTGG 2 cut(s) 148, 221
BseRI GAGGAG 5 cut(s) 38, 41, 44, 47, 618
BseX3I CGGCCG 1 cut(s) 226
BseYI CCCAGC 1 cut(s) 256
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 5 cut(s) 26, 142, 220, 228, 367
BshNI GGYRCC 2 cut(s) 127, 293
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 1 cut(s) 474
BsiSI CCGG 5 cut(s) 125, 131, 350, 364, 507
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 3 cut(s) 130, 196, 283
BsmAI GTCTC 2 cut(s) 475, 630
BsmFI GGGAC 1 cut(s) 299
BsmI GAATGC 3 cut(s) 335, 357, 696
BsnI GGCC 5 cut(s) 26, 142, 220, 228, 367
Bso31I GGTCTC 2 cut(s) 475, 630
Bsp119I TTCGAA 1 cut(s) 700
Bsp1286I GDGCHC 2 cut(s) 190, 474
Bsp143I GATC 1 cut(s) 202
Bsp19I CCATGG 2 cut(s) 21, 33
BspACI CCGC 5 cut(s) 80, 101, 111, 134, 229
BspANI GGCC 5 cut(s) 26, 142, 220, 228, 367
BspHI TCATGA 1 cut(s) 168
BspLI GGNNCC 4 cut(s) 129, 143, 187, 295
BspPI GGATC 1 cut(s) 210
BspT104I TTCGAA 1 cut(s) 700
BspT107I GGYRCC 2 cut(s) 127, 293
BspTNI GGTCTC 2 cut(s) 475, 630
BsrDI GCAATG 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 2 cut(s) 148, 221
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 3 cut(s) 21, 33, 312
BssMI GATC 1 cut(s) 202
BssNI GRCGYC 1 cut(s) 65
BssT1I CCWWGG 3 cut(s) 21, 33, 312
Bst2UI CCWGG 1 cut(s) 478
Bst6I CTCTTC 1 cut(s) 170
BstACI GRCGYC 1 cut(s) 65
BstBAI YACGTR 1 cut(s) 409
BstBI TTCGAA 1 cut(s) 700
BstC8I GCNNGC 2 cut(s) 254, 674
BstDSI CCRYGG 2 cut(s) 21, 33
BstF5I GGATG 2 cut(s) 562, 574
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 1 cut(s) 386
BstKTI GATC 1 cut(s) 205
BstMAI GTCTC 2 cut(s) 475, 630
BstMBI GATC 1 cut(s) 202
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 3 cut(s) 134, 262, 373
BstNI CCWGG 1 cut(s) 478
BstSCI CCNGG 2 cut(s) 123, 476
BstX2I RGATCY 1 cut(s) 202
BstXI CCANNNNNNTGG 1 cut(s) 34
BstYI RGATCY 1 cut(s) 202
BstZI CGGCCG 1 cut(s) 226
BsuRI GGCC 5 cut(s) 26, 142, 220, 228, 367
BtgI CCRYGG 2 cut(s) 21, 33
BtrI CACGTC 1 cut(s) 469
BtsCI GGATG 2 cut(s) 562, 574
Cac8I GCNNGC 2 cut(s) 254, 674
CciI TCATGA 1 cut(s) 168
CfoI GCGC 1 cut(s) 386
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 2 cut(s) 141, 219
CseI GACGC 1 cut(s) 73
Csp6I GTAC 3 cut(s) 211, 301, 457
CviAII CATG 8 cut(s) 22, 28, 34, 169, 340, 521, 668, 706
CviQI GTAC 3 cut(s) 211, 301, 457
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
EaeI YGGCCR 2 cut(s) 24, 226
EagI CGGCCG 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
EciI GGCGGA 1 cut(s) 100
EclXI CGGCCG 1 cut(s) 226
Eco130I CCWWGG 3 cut(s) 21, 33, 312
Eco24I GRGCYC 1 cut(s) 190
Eco31I GGTCTC 2 cut(s) 475, 630
Eco52I CGGCCG 1 cut(s) 226
Eco72I CACGTG 1 cut(s) 409
EcoRII CCWGG 1 cut(s) 476
EcoT14I CCWWGG 3 cut(s) 21, 33, 312
EcoT22I ATGCAT 1 cut(s) 345
EcoT38I GRGCYC 1 cut(s) 190
ErhI CCWWGG 3 cut(s) 21, 33, 312
FaeI CATG 8 cut(s) 25, 31, 37, 172, 343, 524, 671, 709
FaqI GGGAC 1 cut(s) 299
FatI CATG 8 cut(s) 21, 27, 33, 168, 339, 520, 667, 705
Fnu4HI GCNGC 3 cut(s) 80, 135, 229
FokI GGATG 2 cut(s) 569, 581
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 3 cut(s) 80, 135, 229
GlaI GCGC 1 cut(s) 385
GluI GCNGC 3 cut(s) 80, 135, 229
GsaI CCCAGC 1 cut(s) 260
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 5 cut(s) 26, 142, 220, 228, 367
HapII CCGG 5 cut(s) 125, 131, 350, 364, 507
HgaI GACGC 1 cut(s) 73
HhaI GCGC 1 cut(s) 386
Hin1I GRCGYC 1 cut(s) 65
Hin1II CATG 8 cut(s) 25, 31, 37, 172, 343, 524, 671, 709
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HinfI GANTC 4 cut(s) 107, 317, 605, 644
HpaII CCGG 5 cut(s) 125, 131, 350, 364, 507
HphI GGTGA 2 cut(s) 259, 466
Hpy166II GTNNAC 1 cut(s) 412
Hpy188I TCNGA 2 cut(s) 118, 322
Hpy188III TCNNGA 2 cut(s) 61, 169
Hpy8I GTNNAC 1 cut(s) 412
Hpy99I CGWCG 4 cut(s) 67, 91, 122, 383
HpyAV CCTTC 1 cut(s) 433
HpyCH4IV ACGT 2 cut(s) 408, 468
HpyCH4V TGCA 4 cut(s) 193, 333, 343, 426
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 262, 373
HpySE526I ACGT 2 cut(s) 408, 468
Hsp92I GRCGYC 1 cut(s) 65
Hsp92II CATG 8 cut(s) 25, 31, 37, 172, 343, 524, 671, 709
HspAI GCGC 1 cut(s) 384
Kzo9I GATC 1 cut(s) 202
LmnI GCTCC 4 cut(s) 185, 542, 577, 693
LweI GCATC 2 cut(s) 547, 559
MaeII ACGT 2 cut(s) 408, 468
MaeIII GTNAC 1 cut(s) 649
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 2 cut(s) 35, 157
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 2 cut(s) 190, 474
MlsI TGGCCA 1 cut(s) 26
MluCI AATT 1 cut(s) 388
MluNI TGGCCA 1 cut(s) 26
MlyI GAGTC 2 cut(s) 116, 311
MmeI TCCRAC 3 cut(s) 41, 141, 345
Mox20I TGGCCA 1 cut(s) 26
Mph1103I ATGCAT 1 cut(s) 345
MscI TGGCCA 1 cut(s) 26
MslI CAYNNNNRTG 2 cut(s) 26, 32
Msp20I TGGCCA 1 cut(s) 26
MspA1I CMGCKG 2 cut(s) 82, 256
MspI CCGG 5 cut(s) 125, 131, 350, 364, 507
MspR9I CCNGG 2 cut(s) 125, 478
Mva1269I GAATGC 3 cut(s) 335, 357, 696
MvaI CCWGG 1 cut(s) 478
MwoI GCNNNNNNNGC 3 cut(s) 134, 262, 373
NciI CCSGG 1 cut(s) 125
NcoI CCATGG 2 cut(s) 21, 33
NdeII GATC 1 cut(s) 202
NlaIII CATG 8 cut(s) 25, 31, 37, 172, 343, 524, 671, 709
NlaIV GGNNCC 4 cut(s) 129, 143, 187, 295
NmuCI GTSAC 1 cut(s) 649
NsiI ATGCAT 1 cut(s) 345
NspV TTCGAA 1 cut(s) 700
PagI TCATGA 1 cut(s) 168
PctI GAATGC 3 cut(s) 335, 357, 696
PfeI GAWTC 2 cut(s) 605, 644
PkrI GCNGC 3 cut(s) 81, 136, 230
PleI GAGTC 2 cut(s) 115, 311
PmaCI CACGTG 1 cut(s) 409
PmlI CACGTG 1 cut(s) 409
PpsI GAGTC 2 cut(s) 115, 311
Ppu21I YACGTR 1 cut(s) 409
Psp6I CCWGG 1 cut(s) 476
PspCI CACGTG 1 cut(s) 409
PspFI CCCAGC 1 cut(s) 256
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 4 cut(s) 129, 143, 187, 295
PspPI GGNCC 2 cut(s) 141, 219
PsuI RGATCY 1 cut(s) 202
PvuII CAGCTG 1 cut(s) 256
RsaI GTAC 3 cut(s) 212, 302, 458
RsaNI GTAC 3 cut(s) 211, 301, 457
RseI CAYNNNNRTG 2 cut(s) 26, 32
SatI GCNGC 3 cut(s) 80, 135, 229
Sau3AI GATC 1 cut(s) 202
Sau96I GGNCC 2 cut(s) 141, 219
SchI GAGTC 2 cut(s) 116, 311
ScrFI CCNGG 2 cut(s) 125, 478
SduI GDGCHC 2 cut(s) 190, 474
SfaNI GCATC 2 cut(s) 547, 559
SfuI TTCGAA 1 cut(s) 700
SgrAI CRCCGGYG 1 cut(s) 130
SmiMI CAYNNNNRTG 2 cut(s) 26, 32
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 1 cut(s) 388
SsiI CCGC 5 cut(s) 80, 101, 111, 134, 229
StyD4I CCNGG 2 cut(s) 123, 476
StyI CCWWGG 3 cut(s) 21, 33, 312
TaiI ACGT 2 cut(s) 411, 471
TaqI TCGA 4 cut(s) 279, 378, 534, 700
TasI AATT 1 cut(s) 388
TauI GCSGC 3 cut(s) 82, 137, 231
TfiI GAWTC 2 cut(s) 605, 644
TseFI GTSAC 1 cut(s) 649
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 3 cut(s) 157, 185, 702
Zsp2I ATGCAT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.