pycom06g12060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001918
Physical Location & Seq
Forward (+)
17240652 .. 17241975
1324 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g12060.2

Sequence Viewer

Length: 723 bp
ATGACCTCTACAAGTCCGACCATGTCCATGTCCATGTCCTTGCCTTTTCTCCTCCTCCTCCTTCTGACGCCACTCAATCTGCCACTGACGACGATGATGGCGGCGGTAGAGTCCGCCTCCGACGACCCGGCACCGGCGGCGTGGCCCCACCAGTTCCACTCTGTCCTCTTCATGAACAACAGCAAGGGAGCCCTGCAGGTGGTGGATCTCTGGTACGACTGGCCCAACGGCCGCAACTTCAACATAATCCAAAGCCAGCTGGGGAAGCTCACCTACGACCTCGAATGGGACAATGGCACCTCGTACATCTACACCTTGGACTCCGACAGAGAATGCAACACCATGCATTTTCCGGTTGGCATTCTCCGGCCTGATTGGCTCGACGGCGCTAATTATCTGGGTCAGCGACACGTGGACGGGTTTCTGTGCAATGTGTGGGAGAAGGTTGATTTTATTTGGTACTGTGAGGATGTTCTCACTAAAAGACCCGTCCATTGGGTTTTCTACACAGGATATAATGCTCACGTGATGACATTCGAGGTGGGAGCTGTGCTTGAGGATGCCAAGTGGGATGCTCCTGCTCATTGTTTTGGGGAGGAAAAGACTGATTCAGCACAAAGGAGGAGGTCTCCTCCACTTCTACTTGAATCTGTGACAAGTGATTATTCTCATGGCAGGCTGATAAGAGATGAGAGGAGCATTGGAAACCTGGATAGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

27.42

Weight (kDa)

4.87

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 187
Acc36I ACCTGC 1 cut(s) 187
AccB1I GGYRCC 2 cut(s) 130, 296
AciI CCGC 5 cut(s) 101, 104, 114, 137, 232
AclWI GGATC 1 cut(s) 213
AcoI YGGCCR 1 cut(s) 229
AcvI CACGTG 2 cut(s) 412, 526
AcyI GRCGYC 1 cut(s) 68
AfaI GTAC 3 cut(s) 215, 305, 461
AfiI CCNNNNNNNGG 4 cut(s) 133, 199, 286, 495
AflIII ACRYGT 1 cut(s) 409
AgsI TTSAA 2 cut(s) 241, 647
AjnI CCWGG 1 cut(s) 708
AluBI AGCT 4 cut(s) 259, 268, 548, 717
AluI AGCT 4 cut(s) 259, 268, 548, 717
Alw26I GTCTC 1 cut(s) 633
AlwI GGATC 1 cut(s) 213
AoxI GGCC 4 cut(s) 143, 221, 229, 368
AspLEI GCGC 1 cut(s) 389
AspS9I GGNCC 2 cut(s) 144, 222
AsuC2I CCSGG 1 cut(s) 128
AsuHPI GGTGA 1 cut(s) 262
BanI GGYRCC 2 cut(s) 130, 296
BanII GRGCYC 1 cut(s) 193
BarI GAAGNNNNNNTAC 2 cut(s) 257, 289
BbrPI CACGTG 2 cut(s) 412, 526
BccI CCATC 1 cut(s) 91
BceAI ACGGC 2 cut(s) 244, 400
BciT130I CCWGG 1 cut(s) 710
BcnI CCSGG 1 cut(s) 128
BcoDI GTCTC 1 cut(s) 633
BfmI CTRYAG 1 cut(s) 194
BfoI RGCGCY 1 cut(s) 390
BfuAI ACCTGC 1 cut(s) 187
BglI GCCNNNNNGGC 1 cut(s) 376
BisI GCNGC 3 cut(s) 102, 138, 232
BlsI GCNGC 3 cut(s) 103, 139, 233
Bme1390I CCNGG 2 cut(s) 128, 710
BmgT120I GGNCC 2 cut(s) 144, 222
BmiI GGNNCC 4 cut(s) 132, 146, 190, 298
BmrFI CCNGG 2 cut(s) 128, 710
BmsI GCATC 2 cut(s) 550, 562
BplI GAGNNNNNCTC 6 cut(s) 101, 133, 613, 645, 616, 648
BpuEI CTTGAG 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 128
BsaAI YACGTR 2 cut(s) 412, 526
BsaHI GRCGYC 1 cut(s) 68
BsaI GGTCTC 1 cut(s) 633
BsaJI CCNNGG 1 cut(s) 315
BsaWI WCCGGW 1 cut(s) 352
Bsc4I CCNNNNNNNGG 4 cut(s) 133, 199, 286, 495
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 2 cut(s) 151, 224
Bse3DI GCAATG 1 cut(s) 436
BseBI CCWGG 1 cut(s) 710
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 3 cut(s) 475, 565, 577
BseLI CCNNNNNNNGG 4 cut(s) 133, 199, 286, 495
BseMI GCAATG 1 cut(s) 436
BseNI ACTGG 2 cut(s) 151, 224
BseRI GAGGAG 6 cut(s) 41, 44, 47, 621, 637, 709
BseX3I CGGCCG 1 cut(s) 229
BseYI CCCAGC 1 cut(s) 259
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 4 cut(s) 145, 223, 231, 370
BshNI GGYRCC 2 cut(s) 130, 296
BsiEI CGRYCG 1 cut(s) 232
BsiSI CCGG 4 cut(s) 128, 134, 353, 367
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 4 cut(s) 133, 199, 286, 495
BsmAI GTCTC 1 cut(s) 633
BsmFI GGGAC 1 cut(s) 302
BsmI GAATGC 2 cut(s) 338, 360
BsnI GGCC 4 cut(s) 145, 223, 231, 370
Bso31I GGTCTC 1 cut(s) 633
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 1 cut(s) 205
BspACI CCGC 5 cut(s) 101, 104, 114, 137, 232
BspANI GGCC 4 cut(s) 145, 223, 231, 370
BspHI TCATGA 1 cut(s) 171
BspLI GGNNCC 4 cut(s) 132, 146, 190, 298
BspMAI CTGCAG 1 cut(s) 198
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 1 cut(s) 213
BspT107I GGYRCC 2 cut(s) 130, 296
BspTNI GGTCTC 1 cut(s) 633
BsrDI GCAATG 1 cut(s) 436
BsrFI RCCGGY 1 cut(s) 133
BsrI ACTGG 2 cut(s) 151, 224
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 1 cut(s) 205
BssNI GRCGYC 1 cut(s) 68
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 1 cut(s) 710
Bst4CI ACNGT 1 cut(s) 464
Bst6I CTCTTC 1 cut(s) 173
BstACI GRCGYC 1 cut(s) 68
BstBAI YACGTR 2 cut(s) 412, 526
BstC8I GCNNGC 2 cut(s) 257, 677
BstF5I GGATG 3 cut(s) 475, 565, 577
BstH2I RGCGCY 1 cut(s) 390
BstHHI GCGC 1 cut(s) 389
BstKTI GATC 1 cut(s) 208
BstMAI GTCTC 1 cut(s) 633
BstMBI GATC 1 cut(s) 205
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 3 cut(s) 137, 265, 376
BstNI CCWGG 1 cut(s) 710
BstSCI CCNGG 2 cut(s) 126, 708
BstSFI CTRYAG 1 cut(s) 194
BstX2I RGATCY 1 cut(s) 205
BstYI RGATCY 1 cut(s) 205
BstZI CGGCCG 1 cut(s) 229
BsuRI GGCC 4 cut(s) 145, 223, 231, 370
BtsCI GGATG 3 cut(s) 475, 565, 577
BtsIMutI CAGTG 1 cut(s) 83
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 2 cut(s) 257, 677
CciI TCATGA 1 cut(s) 171
CfoI GCGC 1 cut(s) 389
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 2 cut(s) 144, 222
CseI GACGC 1 cut(s) 76
Csp6I GTAC 3 cut(s) 214, 304, 460
CviAII CATG 6 cut(s) 22, 28, 34, 172, 343, 671
CviQI GTAC 3 cut(s) 214, 304, 460
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
EaeI YGGCCR 1 cut(s) 229
EagI CGGCCG 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
EciI GGCGGA 1 cut(s) 103
EclXI CGGCCG 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 193
Eco31I GGTCTC 1 cut(s) 633
Eco52I CGGCCG 1 cut(s) 229
Eco72I CACGTG 2 cut(s) 412, 526
EcoRII CCWGG 1 cut(s) 708
EcoT14I CCWWGG 1 cut(s) 315
EcoT22I ATGCAT 1 cut(s) 348
EcoT38I GRGCYC 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 6 cut(s) 25, 31, 37, 175, 346, 674
FaiI YATR 8 cut(s) 23, 29, 35, 173, 245, 344, 516, 672
FaqI GGGAC 1 cut(s) 302
FatI CATG 6 cut(s) 21, 27, 33, 171, 342, 670
Fnu4HI GCNGC 3 cut(s) 102, 138, 232
FokI GGATG 3 cut(s) 482, 572, 584
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 3 cut(s) 102, 138, 232
GlaI GCGC 1 cut(s) 388
GluI GCNGC 3 cut(s) 102, 138, 232
GsaI CCCAGC 1 cut(s) 263
HaeII RGCGCY 1 cut(s) 390
HaeIII GGCC 4 cut(s) 145, 223, 231, 370
HapII CCGG 4 cut(s) 128, 134, 353, 367
HgaI GACGC 1 cut(s) 76
HhaI GCGC 1 cut(s) 389
Hin1I GRCGYC 1 cut(s) 68
Hin1II CATG 6 cut(s) 25, 31, 37, 175, 346, 674
Hin6I GCGC 1 cut(s) 387
HinP1I GCGC 1 cut(s) 387
HinfI GANTC 4 cut(s) 110, 320, 608, 647
HpaII CCGG 4 cut(s) 128, 134, 353, 367
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 4 cut(s) 18, 66, 121, 325
Hpy188III TCNNGA 1 cut(s) 172
Hpy8I GTNNAC 1 cut(s) 415
Hpy99I CGWCG 3 cut(s) 94, 125, 386
HpyAV CCTTC 2 cut(s) 71, 436
HpyCH4III ACNGT 1 cut(s) 464
HpyCH4IV ACGT 2 cut(s) 411, 525
HpyCH4V TGCA 4 cut(s) 196, 336, 346, 429
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 265, 376
HpySE526I ACGT 2 cut(s) 411, 525
Hsp92I GRCGYC 1 cut(s) 68
Hsp92II CATG 6 cut(s) 25, 31, 37, 175, 346, 674
HspAI GCGC 1 cut(s) 387
Kzo9I GATC 1 cut(s) 205
LmnI GCTCC 4 cut(s) 188, 545, 580, 696
LweI GCATC 2 cut(s) 550, 562
MaeII ACGT 2 cut(s) 411, 525
MaeIII GTNAC 1 cut(s) 652
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MboII GAAGA 1 cut(s) 160
MflI RGATCY 1 cut(s) 205
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 1 cut(s) 391
MlyI GAGTC 2 cut(s) 119, 314
MmeI TCCRAC 3 cut(s) 41, 144, 348
Mph1103I ATGCAT 1 cut(s) 348
MslI CAYNNNNRTG 2 cut(s) 26, 32
MspA1I CMGCKG 1 cut(s) 259
MspI CCGG 4 cut(s) 128, 134, 353, 367
MspR9I CCNGG 2 cut(s) 128, 710
Mva1269I GAATGC 2 cut(s) 338, 360
MvaI CCWGG 1 cut(s) 710
MwoI GCNNNNNNNGC 3 cut(s) 137, 265, 376
NciI CCSGG 1 cut(s) 128
NdeII GATC 1 cut(s) 205
NlaIII CATG 6 cut(s) 25, 31, 37, 175, 346, 674
NlaIV GGNNCC 4 cut(s) 132, 146, 190, 298
NmuCI GTSAC 1 cut(s) 652
NsiI ATGCAT 1 cut(s) 348
PagI TCATGA 1 cut(s) 171
PaqCI CACCTGC 1 cut(s) 187
PctI GAATGC 2 cut(s) 338, 360
PfeI GAWTC 2 cut(s) 608, 647
PflFI GACNNNGTC 1 cut(s) 22
PkrI GCNGC 3 cut(s) 103, 139, 233
PleI GAGTC 2 cut(s) 118, 314
PmaCI CACGTG 2 cut(s) 412, 526
PmlI CACGTG 2 cut(s) 412, 526
PpsI GAGTC 2 cut(s) 118, 314
Ppu21I YACGTR 2 cut(s) 412, 526
Psp6I CCWGG 1 cut(s) 708
PspCI CACGTG 2 cut(s) 412, 526
PspFI CCCAGC 1 cut(s) 259
PspGI CCWGG 1 cut(s) 708
PspN4I GGNNCC 4 cut(s) 132, 146, 190, 298
PspPI GGNCC 2 cut(s) 144, 222
PstI CTGCAG 1 cut(s) 198
PsuI RGATCY 1 cut(s) 205
PsyI GACNNNGTC 1 cut(s) 22
PvuII CAGCTG 1 cut(s) 259
RsaI GTAC 3 cut(s) 215, 305, 461
RsaNI GTAC 3 cut(s) 214, 304, 460
RseI CAYNNNNRTG 2 cut(s) 26, 32
SatI GCNGC 3 cut(s) 102, 138, 232
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 2 cut(s) 144, 222
SbfI CCTGCAGG 1 cut(s) 198
SchI GAGTC 2 cut(s) 119, 314
ScrFI CCNGG 2 cut(s) 128, 710
SdaI CCTGCAGG 1 cut(s) 198
SduI GDGCHC 1 cut(s) 193
SfaNI GCATC 2 cut(s) 550, 562
SfcI CTRYAG 1 cut(s) 194
SgrAI CRCCGGYG 1 cut(s) 133
SmiMI CAYNNNNRTG 2 cut(s) 26, 32
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse8387I CCTGCAGG 1 cut(s) 198
Sse9I AATT 1 cut(s) 391
SsiI CCGC 5 cut(s) 101, 104, 114, 137, 232
StyD4I CCNGG 2 cut(s) 126, 708
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 1 cut(s) 464
TaiI ACGT 2 cut(s) 414, 528
TaqI TCGA 3 cut(s) 282, 381, 537
TasI AATT 1 cut(s) 391
TauI GCSGC 3 cut(s) 104, 140, 234
TfiI GAWTC 2 cut(s) 608, 647
TscAI CASTG 1 cut(s) 90
TseFI GTSAC 1 cut(s) 652
Tsp45I GTSAC 1 cut(s) 652
TspDTI ATGAA 2 cut(s) 160, 188
TspRI CASTG 1 cut(s) 90
Tth111I GACNNNGTC 1 cut(s) 22
Zsp2I ATGCAT 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.