Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001900

Physical Location & Seq

Genomic Coordinates Forward (+)

28945718 .. 28948814

Gene Structure 3097 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1147300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1668 bp

ATGGCGGACGAATCCACACCAAATCCCAAGAGCGGCTACTGCCTACAAACCAAGACCTTCCATACTCTCCGGCCTCAGGTCCCACTCCCGCCACTCTCCCAACCCCTCTCCCTCACCCAGTTCACCCTCTCCCTCCTCCAATCCTCTACGGCCTCGCCCACCACCTCCGTCCTCATCGACGCCGCCTCCGACCGCCATGTTTCCTACCAAACTTTCCTCGGCCAAATCCACTCCCTCACTCTCTCCCTCCAATCCCTTGCCCCCTCTCTCTCCAAAGGTCACGTGGCATTCATTCTCTCCCCTCCTTCCCTCCACGTCCCCGTCCTCTATTTCTCTCTCCTCGCCTTAGGCGTCGTCGTTTCGCCAACTAATCCTGTCGGGTCCGAGTCCGAGATCGCCCACCAGGTCCGGCTAACCCGACCAGTCATCGCCTTCGCCACGTCAGCAACCGCTCAGAAGCTCCCCAAGGGTAACGACAAAATCCGCAACGTTATACTCCTCGACTCGCCCGAATTCCTCTCCATGCTCAACCGGGTCGGTTCATCCGAAACACGACCAGACCTCAGGTTTACAGTCAACCAGACCGACCCGGCTACGATTCTCTTCTCCTCCGGCACCACCGGCCGAGTCAAAGGCGTTCTCTTATCTCACCGCAACTTCATCGCCGTCATAGCTGGGCTCCACGCCCTTCGCTCACCCGACCCGGACCTACCTAACAGCAAAGCGGTGTCGCTGTTCACGCTGCCTCTGTTCCACGTGTTCGGGTTCTTCATGGTGGTTCGGGCGGTGGCAATGGGTGAGACCTTGGTTTTGATGGAGAGGTTCGACTTCGAATCAATGCTGAGCGCCGTGGACCGGCACAAAATCTCGTACATGCCGGTATCGCCGCCGCTGGTCGTTGCAATGGCGAAGTCGGAGCTGGTTAAGAAATACGATCTCAGCTCGCTTCGGCTTCTCCGCTGTGGCGGTGCGCCGCTGGGAAAGGACGTTGTTGACAGATTCCGTGAGAGATTTCCCACTGTTGAAATTGTACAGGGATATGGTTTGACGGAGACCGGAGCAGGGGCGACGAGGATGATAGACTCCGAAGAGACTAAGTCGCATGCTTCTGTGGGCCGCCTATCGGAAAATATGGAAGCCAAGATTGTCGACCCTGAAACAGGAGAGGCATTGCCTCCCGGCAAGAGGGGTGAGCTATGGCTGCGAGGTCCATCGGTCATGAAAGGTTATGTAGGAGATGAGAAGGCAACTGCTGAGACCTTGGACAAAGACGGGTGGTTGAAGACCGGTGATCTTTGCTATTTCGATGACGAGGGGTTCCTCTACATTGTTGACAGGTTGAAGGAATTGATAAAGTACAAGGCATATCAGGTACCTCCAGCTGAGCTGGAACATATACTTCTAACCCATCCTGACATTGCCGATGCTGCTGTGATTCCGTATCCGGATGAAGATGCAGGGGAGATTCCCATGGCCTATGTGGTTAGAAAGCCCGGAAGCAACATCACCGAGGTTCTAGTTATGGATTTCGTTGCAAAACAAGTTTCACCGTACAAGAAGATACGGCGGGTAGCGTTTATCAACTCCATTCCAAAATCTCCAGCAGGGAAGATCTTGAGGAGGGAGCTAGTCAATCATGCTTTATCTAGTAGTTCATCTAAATTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004321 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0008150 GO:0008152 GO:0009058 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0016053 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0032787 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

556

Amino Acids

60.68

Weight (kDa)

8.22

Isoelectric Point (pI)

45.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	52 - 410	1.2e-76	AMP-binding enzyme
AMP-binding_C	PF13193	462 - 537	1.5e-17	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63380
fragaria_vesca	FvH4_4g24170 FvH4_5g07750 FvH4_5g07750 FvH4_5g07750
malus_domestica	MD06G1147300.v1.1 MD06G1147500.v1.1 MD13G1113700.v1.1 MD14G1161200.v1.1 MD14G1161300.v1.1 MD16G1112900.v1.1 MD16G1113000.v1.1
prunus_persica	Prupe.1G232500_v2.0.a1 Prupe.5G154000_v2.0.a1 Prupe.5G154100_v2.0.a1
pyrus_communis	pycom06g13680 pycom06g13690 pycom13g09900 pycom14g13410 pycom16g09570 pycom16g09580
rosa_chinensis	RchiOBHm_Chr4g0430951 RchiOBHm_Chr7g0190671 RchiOBHm_Chr7g0190681
rosa_laevigata	RLG00000004504 RLG00000006935
rosa_multiflora	Rmu_co8020444.1_g000001 Rmu_sc0006263.1_g000015 Rmu_sc0006709.1_g000003 Rmu_sc0013571.1_g000005
rosa_roxburghii	Rroxscaffold_3G00264410 Rroxscaffold_5G00372640
rosa_rugosa	Rorug04G0247400 Rorug06G0506300 Rorug06G0506400
rosa_samantha	Rh4AG303200 Rh4AG304100 Rh4BG311400 Rh4CG327700 Rh4DG307500 Rh7AG112900 Rh7BG115400 Rh7CG117900 Rh7DG116800
rosa_wichuraiana	Rw4G026360 Rw7G009850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1372
AccB1I	GGYRCC	2 cut(s)	614, 1372
AccBSI	CCGCTC	2 cut(s)	33, 452
AccI	GTMKAC	1 cut(s)	1149
AccIII	TCCGGA	1 cut(s)	1444
AclI	AACGTT	1 cut(s)	489
AcoI	YGGCCR	2 cut(s)	220, 622
AcsI	RAATTY	1 cut(s)	512
AcvI	CACGTG	2 cut(s)	283, 757
AcyI	GRCGYC	2 cut(s)	180, 351
AfaI	GTAC	5 cut(s)	872, 1032, 1358, 1374, 1553
AfiI	CCNNNNNNNGG	7 cut(s)	32, 76, 855, 1062, 1123, 1160, 1185
AflIII	ACRYGT	1 cut(s)	756
AgeI	ACCGGT	1 cut(s)	1286
AgsI	TTSAA	3 cut(s)	1025, 1282, 1342
AjiI	CACGTC	2 cut(s)	316, 441
AjnI	CCWGG	1 cut(s)	402
AluBI	AGCT	8 cut(s)	460, 674, 919, 942, 1195, 1382, 1387, 1627
AluI	AGCT	8 cut(s)	460, 674, 919, 942, 1195, 1382, 1387, 1627
Alw26I	GTCTC	4 cut(s)	794, 1046, 1085, 1250
Aor13HI	TCCGGA	1 cut(s)	1444
AoxI	GGCC	6 cut(s)	71, 150, 220, 622, 1114, 1473
ApeKI	GCWGC	3 cut(s)	742, 1201, 1427
ApoI	RAATTY	1 cut(s)	512
ArsI	GACNNNNNNTTYG	2 cut(s)	793, 825
AsiGI	ACCGGT	1 cut(s)	1286
Asp718I	GGTACC	1 cut(s)	1372
AspLEI	GCGC	2 cut(s)	848, 973
AspS9I	GGNCC	7 cut(s)	79, 381, 406, 706, 853, 1114, 1208
AsuC2I	CCSGG	5 cut(s)	533, 590, 704, 1179, 1494
AsuHPI	GGTGA	9 cut(s)	106, 115, 641, 687, 809, 1202, 1301, 1498, 1539
AsuII	TTCGAA	1 cut(s)	831
AvaII	GGWCC	6 cut(s)	79, 381, 406, 706, 853, 1208
AxyI	CCTNAGG	3 cut(s)	75, 346, 563
BanI	GGYRCC	2 cut(s)	614, 1372
BanII	GRGCYC	1 cut(s)	681
BbrPI	CACGTG	2 cut(s)	283, 757
BbsI	GAAGAC	1 cut(s)	1289
BbvI	GCAGC	3 cut(s)	729, 1188, 1414
BccI	CCATC	3 cut(s)	808, 1219, 1416
BceAI	ACGGC	4 cut(s)	165, 650, 833, 1580
BcgI	CGANNNNNNTGC	2 cut(s)	643, 677
BciT130I	CCWGG	1 cut(s)	404
BciVI	GTATCC	1 cut(s)	1452
BcnI	CCSGG	5 cut(s)	533, 590, 704, 1179, 1494
BcoDI	GTCTC	4 cut(s)	794, 1046, 1085, 1250
BfaI	CTAG	3 cut(s)	1517, 1628, 1647
BfoI	RGCGCY	1 cut(s)	849
BfuI	GTATCC	1 cut(s)	1452
BglII	AGATCT	1 cut(s)	1611
BisI	GCNGC	9 cut(s)	34, 183, 743, 887, 890, 974, 1117, 1202, 1428
BlpI	GCTNAGC	2 cut(s)	842, 1383
BlsI	GCNGC	9 cut(s)	35, 184, 744, 888, 891, 975, 1118, 1203, 1429
Bme1390I	CCNGG	6 cut(s)	404, 533, 590, 704, 1179, 1494
Bme18I	GGWCC	6 cut(s)	79, 381, 406, 706, 853, 1208
BmgBI	CACGTC	2 cut(s)	316, 441
BmgT120I	GGNCC	7 cut(s)	79, 381, 406, 706, 853, 1114, 1208
BmiI	GGNNCC	6 cut(s)	81, 382, 616, 680, 1319, 1374
BmrFI	CCNGG	6 cut(s)	404, 533, 590, 704, 1179, 1494
BmrI	ACTGGG	1 cut(s)	112
BmsI	GCATC	2 cut(s)	1414, 1444
BmuI	ACTGGG	1 cut(s)	112
BpiI	GAAGAC	1 cut(s)	1289
BpmI	CTGGAG	2 cut(s)	1362, 1584
Bpu1102I	GCTNAGC	2 cut(s)	842, 1383
Bpu14I	TTCGAA	1 cut(s)	831
BpuEI	CTTGAG	1 cut(s)	1636
BpuMI	CCSGG	5 cut(s)	533, 590, 704, 1179, 1494
BsaAI	YACGTR	2 cut(s)	283, 757
BsaHI	GRCGYC	2 cut(s)	180, 351
BsaI	GGTCTC	3 cut(s)	794, 1046, 1250
BsaJI	CCNNGG	7 cut(s)	217, 465, 804, 849, 1260, 1470, 1509
BsaWI	WCCGGW	3 cut(s)	1055, 1286, 1444
BsaXI	ACNNNNNCTCC	2 cut(s)	170, 200
Bsc4I	CCNNNNNNNGG	7 cut(s)	32, 76, 855, 1062, 1123, 1160, 1185
Bse118I	RCCGGY	4 cut(s)	620, 855, 877, 1286
Bse1I	ACTGG	2 cut(s)	118, 422
Bse21I	CCTNAGG	3 cut(s)	75, 346, 563
Bse3DI	GCAATG	4 cut(s)	798, 909, 1169, 1416
BseAI	TCCGGA	1 cut(s)	1444
BseBI	CCWGG	1 cut(s)	404
BseDI	CCNNGG	7 cut(s)	217, 465, 804, 849, 1260, 1470, 1509
BseGI	GGATG	4 cut(s)	542, 1080, 1408, 1453
BseLI	CCNNNNNNNGG	7 cut(s)	32, 76, 855, 1062, 1123, 1160, 1185
BseMI	GCAATG	4 cut(s)	798, 909, 1169, 1416
BseMII	CTCAG	7 cut(s)	89, 467, 577, 833, 952, 1245, 1374
BseNI	ACTGG	2 cut(s)	118, 422
BseRI	GAGGAG	5 cut(s)	125, 329, 488, 598, 1633
BseX3I	CGGCCG	1 cut(s)	622
BseXI	GCAGC	3 cut(s)	729, 1188, 1414
BseYI	CCCAGC	2 cut(s)	674, 976
Bsh1285I	CGRYCG	2 cut(s)	193, 625
BshFI	GGCC	6 cut(s)	73, 152, 222, 624, 1116, 1475
BshNI	GGYRCC	2 cut(s)	614, 1372
BshTI	ACCGGT	1 cut(s)	1286
BsiEI	CGRYCG	2 cut(s)	193, 625
BslFI	GGGAC	2 cut(s)	65, 302
BslI	CCNNNNNNNGG	7 cut(s)	32, 76, 855, 1062, 1123, 1160, 1185
BsmAI	GTCTC	4 cut(s)	794, 1046, 1085, 1250
BsmFI	GGGAC	2 cut(s)	65, 302
BsmI	GAATGC	1 cut(s)	287
BsnI	GGCC	6 cut(s)	73, 152, 222, 624, 1116, 1475
Bso31I	GGTCTC	3 cut(s)	794, 1046, 1250
Bsp119I	TTCGAA	1 cut(s)	831
Bsp1286I	GDGCHC	1 cut(s)	681
Bsp13I	TCCGGA	1 cut(s)	1444
Bsp1407I	TGTACA	1 cut(s)	1030
Bsp143I	GATC	4 cut(s)	393, 934, 1291, 1611
Bsp1720I	GCTNAGC	2 cut(s)	842, 1383
Bsp19I	CCATGG	1 cut(s)	1470
BspANI	GGCC	6 cut(s)	73, 152, 222, 624, 1116, 1475
BspCNI	CTCAG	7 cut(s)	88, 466, 576, 834, 951, 1246, 1375
BspEI	TCCGGA	1 cut(s)	1444
BspHI	TCATGA	1 cut(s)	1218
BspLI	GGNNCC	6 cut(s)	81, 382, 616, 680, 1319, 1374
BspT104I	TTCGAA	1 cut(s)	831
BspT107I	GGYRCC	2 cut(s)	614, 1372
BspTNI	GGTCTC	3 cut(s)	794, 1046, 1250
BsrBI	CCGCTC	2 cut(s)	33, 452
BsrDI	GCAATG	4 cut(s)	798, 909, 1169, 1416
BsrFI	RCCGGY	4 cut(s)	620, 855, 877, 1286
BsrGI	TGTACA	1 cut(s)	1030
BsrI	ACTGG	2 cut(s)	118, 422
BssAI	RCCGGY	4 cut(s)	620, 855, 877, 1286
BssECI	CCNNGG	7 cut(s)	217, 465, 804, 849, 1260, 1470, 1509
BssMI	GATC	4 cut(s)	393, 934, 1291, 1611
BssNI	GRCGYC	2 cut(s)	180, 351
BssT1I	CCWWGG	4 cut(s)	465, 804, 1260, 1470
Bst2UI	CCWGG	1 cut(s)	404
Bst4CI	ACNGT	3 cut(s)	574, 1021, 1551
Bst6I	CTCTTC	2 cut(s)	608, 1083
BstACI	GRCGYC	2 cut(s)	180, 351
BstAUI	TGTACA	1 cut(s)	1030
BstBAI	YACGTR	2 cut(s)	283, 757
BstBI	TTCGAA	1 cut(s)	831
BstC8I	GCNNGC	2 cut(s)	944, 1104
BstDEI	CTNAG	9 cut(s)	75, 346, 453, 563, 842, 938, 1095, 1254, 1383
BstDSI	CCRYGG	2 cut(s)	849, 1470
BstENI	CCTNNNNNAGG	1 cut(s)	1158
BstF5I	GGATG	4 cut(s)	542, 1080, 1408, 1453
BstH2I	RGCGCY	1 cut(s)	849
BstHHI	GCGC	2 cut(s)	848, 973
BstKTI	GATC	4 cut(s)	396, 937, 1294, 1614
BstMAI	GTCTC	4 cut(s)	794, 1046, 1085, 1250
BstMBI	GATC	4 cut(s)	393, 934, 1291, 1611
BstMCI	CGRYCG	2 cut(s)	193, 625
BstMWI	GCNNNNNNNGC	8 cut(s)	39, 443, 621, 671, 739, 883, 1201, 1427
BstNI	CCWGG	1 cut(s)	404
BstNSI	RCATGY	2 cut(s)	877, 1106
BstSCI	CCNGG	6 cut(s)	402, 531, 588, 702, 1177, 1492
BstV1I	GCAGC	3 cut(s)	729, 1188, 1414
BstV2I	GAAGAC	1 cut(s)	1289
BstX2I	RGATCY	1 cut(s)	1611
BstYI	RGATCY	1 cut(s)	1611
BstZI	CGGCCG	1 cut(s)	622
Bsu36I	CCTNAGG	3 cut(s)	75, 346, 563
BsuI	GTATCC	1 cut(s)	1452
BsuRI	GGCC	6 cut(s)	73, 152, 222, 624, 1116, 1475
BtgI	CCRYGG	2 cut(s)	849, 1470
BtgZI	GCGATG	2 cut(s)	412, 646
BtrI	CACGTC	2 cut(s)	316, 441
BtsCI	GGATG	4 cut(s)	542, 1080, 1408, 1453
BtsIMutI	CAGTG	1 cut(s)	1017
Cac8I	GCNNGC	2 cut(s)	944, 1104
CciI	TCATGA	1 cut(s)	1218
CfoI	GCGC	2 cut(s)	848, 973
Cfr10I	RCCGGY	4 cut(s)	620, 855, 877, 1286
Cfr13I	GGNCC	7 cut(s)	79, 381, 406, 706, 853, 1114, 1208
CseI	GACGC	2 cut(s)	188, 340
CsiI	ACCWGGT	1 cut(s)	402
Csp6I	GTAC	5 cut(s)	871, 1031, 1357, 1373, 1552
CspAI	ACCGGT	1 cut(s)	1286
CviAII	CATG	8 cut(s)	197, 523, 772, 874, 1103, 1219, 1471, 1637
CviQI	GTAC	5 cut(s)	871, 1031, 1357, 1373, 1552
DdeI	CTNAG	9 cut(s)	75, 346, 453, 563, 842, 938, 1095, 1254, 1383
DpnI	GATC	4 cut(s)	395, 936, 1293, 1613
DpnII	GATC	4 cut(s)	393, 934, 1291, 1611
EaeI	YGGCCR	2 cut(s)	220, 622
EagI	CGGCCG	1 cut(s)	622
Eam1104I	CTCTTC	2 cut(s)	608, 1083
EarI	CTCTTC	2 cut(s)	608, 1083
EciI	GGCGGA	1 cut(s)	20
EclXI	CGGCCG	1 cut(s)	622
Eco130I	CCWWGG	4 cut(s)	465, 804, 1260, 1470
Eco24I	GRGCYC	1 cut(s)	681
Eco31I	GGTCTC	3 cut(s)	794, 1046, 1250
Eco47I	GGWCC	6 cut(s)	79, 381, 406, 706, 853, 1208
Eco52I	CGGCCG	1 cut(s)	622
Eco72I	CACGTG	2 cut(s)	283, 757
Eco81I	CCTNAGG	3 cut(s)	75, 346, 563
EcoNI	CCTNNNNNAGG	1 cut(s)	1158
EcoO109I	RGGNCCY	1 cut(s)	79
EcoRI	GAATTC	1 cut(s)	512
EcoRII	CCWGG	1 cut(s)	402
EcoT14I	CCWWGG	4 cut(s)	465, 804, 1260, 1470
EcoT38I	GRGCYC	1 cut(s)	681
ErhI	CCWWGG	4 cut(s)	465, 804, 1260, 1470
FaeI	CATG	8 cut(s)	200, 526, 775, 877, 1106, 1222, 1474, 1640
FaqI	GGGAC	2 cut(s)	65, 302
FatI	CATG	8 cut(s)	196, 522, 771, 873, 1102, 1218, 1470, 1636
FauI	CCCGC	2 cut(s)	96, 1560
FblI	GTMKAC	1 cut(s)	1149
Fnu4HI	GCNGC	9 cut(s)	34, 183, 743, 887, 890, 974, 1117, 1202, 1428
FokI	GGATG	4 cut(s)	529, 1087, 1395, 1460
FriOI	GRGCYC	1 cut(s)	681
Fsp4HI	GCNGC	9 cut(s)	34, 183, 743, 887, 890, 974, 1117, 1202, 1428
FspBI	CTAG	3 cut(s)	1517, 1628, 1647
GlaI	GCGC	2 cut(s)	847, 972
GluI	GCNGC	9 cut(s)	34, 183, 743, 887, 890, 974, 1117, 1202, 1428
GsaI	CCCAGC	2 cut(s)	678, 980
GsuI	CTGGAG	2 cut(s)	1362, 1584
HaeII	RGCGCY	1 cut(s)	849
HaeIII	GGCC	6 cut(s)	73, 152, 222, 624, 1116, 1475
HgaI	GACGC	2 cut(s)	188, 340
HhaI	GCGC	2 cut(s)	848, 973
Hin1I	GRCGYC	2 cut(s)	180, 351
Hin1II	CATG	8 cut(s)	200, 526, 775, 877, 1106, 1222, 1474, 1640
Hin6I	GCGC	2 cut(s)	846, 971
HinP1I	GCGC	2 cut(s)	846, 971
HincII	GTYRAC	4 cut(s)	577, 994, 1150, 1333
HindII	GTYRAC	4 cut(s)	577, 994, 1150, 1333
HphI	GGTGA	9 cut(s)	106, 115, 641, 687, 809, 1202, 1301, 1498, 1539
Hpy166II	GTNNAC	8 cut(s)	123, 570, 577, 738, 853, 994, 1150, 1333
Hpy188I	TCNGA	8 cut(s)	190, 385, 391, 456, 547, 916, 1087, 1126
Hpy188III	TCNNGA	4 cut(s)	1219, 1412, 1445, 1615
Hpy8I	GTNNAC	8 cut(s)	123, 570, 577, 738, 853, 994, 1150, 1333
Hpy99I	CGWCG	4 cut(s)	182, 356, 359, 1072
HpyAV	CCTTC	6 cut(s)	67, 315, 442, 698, 1237, 1336
HpyCH4III	ACNGT	3 cut(s)	574, 1021, 1551
HpyCH4IV	ACGT	6 cut(s)	282, 315, 440, 489, 756, 987
HpyCH4V	TGCA	3 cut(s)	902, 1457, 1535
HpyF10VI	GCNNNNNNNGC	8 cut(s)	39, 443, 621, 671, 739, 883, 1201, 1427
HpyF3I	CTNAG	9 cut(s)	75, 346, 453, 563, 842, 938, 1095, 1254, 1383
HpySE526I	ACGT	6 cut(s)	282, 315, 440, 489, 756, 987
Hsp92I	GRCGYC	2 cut(s)	180, 351
Hsp92II	CATG	8 cut(s)	200, 526, 775, 877, 1106, 1222, 1474, 1640
HspAI	GCGC	2 cut(s)	846, 971
Kpn2I	TCCGGA	1 cut(s)	1444
KpnI	GGTACC	1 cut(s)	1376
Kzo9I	GATC	4 cut(s)	393, 934, 1291, 1611
LmnI	GCTCC	5 cut(s)	465, 684, 916, 1058, 1624
Lsp1109I	GCAGC	3 cut(s)	729, 1188, 1414
LweI	GCATC	2 cut(s)	1414, 1444
MabI	ACCWGGT	1 cut(s)	402
MaeI	CTAG	3 cut(s)	1517, 1628, 1647
MaeII	ACGT	6 cut(s)	282, 315, 440, 489, 756, 987
MaeIII	GTNAC	2 cut(s)	278, 470
MalI	GATC	4 cut(s)	395, 936, 1293, 1613
MbiI	CCGCTC	2 cut(s)	33, 452
MboI	GATC	4 cut(s)	393, 934, 1291, 1611
MboII	GAAGA	7 cut(s)	595, 760, 1100, 1294, 1463, 1570, 1621
MflI	RGATCY	1 cut(s)	1611
MhlI	GDGCHC	1 cut(s)	681
MluCI	AATT	4 cut(s)	512, 1026, 1346, 1661
MlyI	GAGTC	4 cut(s)	395, 497, 636, 1076
MmeI	TCCRAC	2 cut(s)	213, 894
MroI	TCCGGA	1 cut(s)	1444
MseI	TTAA	1 cut(s)	924
MspA1I	CMGCKG	4 cut(s)	892, 960, 976, 1382
MspR9I	CCNGG	6 cut(s)	404, 533, 590, 704, 1179, 1494
Mva1269I	GAATGC	1 cut(s)	287
MvaI	CCWGG	1 cut(s)	404
MwoI	GCNNNNNNNGC	8 cut(s)	39, 443, 621, 671, 739, 883, 1201, 1427
NciI	CCSGG	5 cut(s)	533, 590, 704, 1179, 1494
NcoI	CCATGG	1 cut(s)	1470
NdeII	GATC	4 cut(s)	393, 934, 1291, 1611
NlaIII	CATG	8 cut(s)	200, 526, 775, 877, 1106, 1222, 1474, 1640
NlaIV	GGNNCC	6 cut(s)	81, 382, 616, 680, 1319, 1374
NmeAIII	GCCGAG	2 cut(s)	198, 650
NmuCI	GTSAC	1 cut(s)	278
NspI	RCATGY	2 cut(s)	877, 1106
NspV	TTCGAA	1 cut(s)	831
PaeI	GCATGC	1 cut(s)	1106
PagI	TCATGA	1 cut(s)	1218
PcsI	WCGNNNNNNNCGW	4 cut(s)	186, 348, 507, 543
PctI	GAATGC	1 cut(s)	287
PfeI	GAWTC	6 cut(s)	11, 598, 833, 999, 1435, 1465
PflFI	GACNNNGTC	1 cut(s)	1096
PinAI	ACCGGT	1 cut(s)	1286
PkrI	GCNGC	9 cut(s)	35, 184, 744, 888, 891, 975, 1118, 1203, 1429
PleI	GAGTC	4 cut(s)	394, 497, 635, 1076
PmaCI	CACGTG	2 cut(s)	283, 757
PmlI	CACGTG	2 cut(s)	283, 757
PpsI	GAGTC	4 cut(s)	394, 497, 635, 1076
Ppu21I	YACGTR	2 cut(s)	283, 757
PpuMI	RGGWCCY	1 cut(s)	79
Psp1406I	AACGTT	1 cut(s)	489
Psp5II	RGGWCCY	1 cut(s)	79
Psp6I	CCWGG	1 cut(s)	402
PspCI	CACGTG	2 cut(s)	283, 757
PspFI	CCCAGC	2 cut(s)	674, 976
PspGI	CCWGG	1 cut(s)	402
PspN4I	GGNNCC	6 cut(s)	81, 382, 616, 680, 1319, 1374
PspPI	GGNCC	7 cut(s)	79, 381, 406, 706, 853, 1114, 1208
PspPPI	RGGWCCY	1 cut(s)	79
PsuI	RGATCY	1 cut(s)	1611
PsyI	GACNNNGTC	1 cut(s)	1096
PvuII	CAGCTG	1 cut(s)	1382
RsaI	GTAC	5 cut(s)	872, 1032, 1358, 1374, 1553
RsaNI	GTAC	5 cut(s)	871, 1031, 1357, 1373, 1552
SalI	GTCGAC	1 cut(s)	1148
SaqAI	TTAA	1 cut(s)	924
SatI	GCNGC	9 cut(s)	34, 183, 743, 887, 890, 974, 1117, 1202, 1428
Sau3AI	GATC	4 cut(s)	393, 934, 1291, 1611
Sau96I	GGNCC	7 cut(s)	79, 381, 406, 706, 853, 1114, 1208
SchI	GAGTC	4 cut(s)	395, 497, 636, 1076
ScrFI	CCNGG	6 cut(s)	404, 533, 590, 704, 1179, 1494
SduI	GDGCHC	1 cut(s)	681
SexAI	ACCWGGT	1 cut(s)	402
SfaNI	GCATC	2 cut(s)	1414, 1444
SfuI	TTCGAA	1 cut(s)	831
SinI	GGWCC	6 cut(s)	79, 381, 406, 706, 853, 1208
SmlI	CTYRAG	1 cut(s)	1615
SmoI	CTYRAG	1 cut(s)	1615
SphI	GCATGC	1 cut(s)	1106
Sse9I	AATT	4 cut(s)	512, 1026, 1346, 1661
SspMI	CTAG	3 cut(s)	1517, 1628, 1647
StyD4I	CCNGG	6 cut(s)	402, 531, 588, 702, 1177, 1492
StyI	CCWWGG	4 cut(s)	465, 804, 1260, 1470
TaaI	ACNGT	3 cut(s)	574, 1021, 1551
TaiI	ACGT	6 cut(s)	285, 318, 443, 492, 759, 990
TaqI	TCGA	6 cut(s)	177, 501, 825, 831, 1149, 1305
TaqII	GACCGA	2 cut(s)	599, 1204
TasI	AATT	4 cut(s)	512, 1026, 1346, 1661
TatI	WGTACW	2 cut(s)	1030, 1356
TauI	GCSGC	6 cut(s)	36, 185, 889, 892, 976, 1119
TfiI	GAWTC	6 cut(s)	11, 598, 833, 999, 1435, 1465
Tru1I	TTAA	1 cut(s)	924
Tru9I	TTAA	1 cut(s)	924
TscAI	CASTG	1 cut(s)	1024
TseFI	GTSAC	1 cut(s)	278
TseI	GCWGC	3 cut(s)	742, 1201, 1427
Tsp45I	GTSAC	1 cut(s)	278
TspDTI	ATGAA	7 cut(s)	280, 531, 649, 760, 1235, 1464, 1644
TspGWI	ACGGA	4 cut(s)	157, 992, 1064, 1428
TspRI	CASTG	1 cut(s)	1024
Tth111I	GACNNNGTC	1 cut(s)	1096
VpaK11BI	GGWCC	6 cut(s)	79, 381, 406, 706, 853, 1208
XagI	CCTNNNNNAGG	1 cut(s)	1158
XapI	RAATTY	1 cut(s)	512
XceI	RCATGY	2 cut(s)	877, 1106
XcmI	CCANNNNNNNNNTGG	2 cut(s)	280, 1477
XmiI	GTMKAC	1 cut(s)	1149
XspI	CTAG	3 cut(s)	1517, 1628, 1647

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.