Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001900

Physical Location & Seq

Genomic Coordinates Reverse (-)

25558629 .. 25561940

Gene Structure 3312 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1161200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1680 bp

ATGGCGGAGGAATCAGCAGCAAATCTCAGACGTTGGATCGATCCGAAGAGCGGCTACTGCCCACGTACCAAGACCTTCCACTCTCTCCGGCCTCCGGTCCCACTCCCGCCGCTCTCGCAACCCCTCTCCCTCACCCAATTCACCCTCTCCCTCCTCCGTTCCTCCATGGCCAAGCCCACCACGTCCGCCCTCATCGACGCCGCCTCCGACCGCCACGTCTCGTACCAAACCTTCCTCAGCCAAATCCACTCCCTCACTCTCTCCCTCCAATCCCTCGCGCTGTTCAAAGGCCACGTGGCCTTCATTCTCTCCCCTCCTTCGCTCCACGTCCCCGTCCTCTATTTCTCCCTCCTCGCCTTCGGCGTCGTCGTTTGTCCCGCCAATCCGATCGGGTCCGAGTCCGAGATCGCCCACCAGGTCCGGCTAACCCGACCCGCCATCGCCTTCGCCACGTCAGCAACCGCCAACAAGCTCCCCAAAGGGGATGATAAAATCCGCACCGTAATCCTCGACTCGCCCGAATTCCTCTCCATGCTCGACCGGGACGGGTCGTCCGTAACCCGATATGAGAGCTCAGTTGAAGTCAACCAAACCGACCCGGCTGCGATTCTTTTCTCCTCCGGCACCACCGGCCGTGTCAAAGGCGTTATGTTGACTCACCGCAATTTCATCGCCCTGTTGGCCGGGCTCCACACCCTTCGGCGCGAACCCGACCCTACCCTACTCGACGGGCAACCCGTATCGCTGTTCACGCTGCCTCTGTTCCACGTGTTCGGGTTCTTCATGGTGGTTCGGGCGGTAGCGTTGGGCGAGACCTTGGTTTTGATGGAGAAGTTCCAGTTCGAAGCGATGCTGAGGGCCGTGGAGAGGCACAAAGTGTGGTACATGCCAGTGTCGCCCCCGTTGATCGTGGCTCTGTCGAAGTCGGAGTTGGCTCAGAAGTACGATCTCAGCTCGCTTCGGCTTCTCGGCTGTGGCGGCGCGCCGCTGGGCAAGGAGGCTGCGGACAGATTTGGTGAGAGATTTCCCAATGTGGAAGTTGTACAGGGATATGGTTTAACCGAGACCGGAGGAGGGGCTACGAGGATGATTGACCCTGAAGAATGTGCGCGTCATGCTTCTGTGGGCCGCCTGGCCGAAAATATGGAAGCCAAGATTGTTGACCCTGAAACCGGAGAGGCCTTGCCTCCTGGCAAGAGGGGTGAGCTATGGCTGCGAGGTCCAACAATCATGAAGGGTTATGTTAGAGATGACAAGGCAACTACTGAGACCTTGGATGAAGATGGGTGGTTGAAGACCGGTGATCTTTGTTACTTTGATGACGAGGGGTTCCTCTACATTGTTGACAGGTTAAAGGAATTGATAAAGTACAAGGCATATCAGGTACCCCCAGCTGAGTTGGAACATATACTTCAATCCCATCCCGACGTTGAGGATGCTGCTGTGATTCCATATCCGGATGAAGACGCGGGGCAGATTCCCATGGCATATGTGGTTAGAAAGCCCGGAAGTAATATCACTGAGGCTCTAGTTATGGATTTCGTTGCAAAACAAGTTGCTCCGTACAAGAAGATACGACGTGTAGCGTTTATCAACTCCATTCCAAAATCTCCAGCAGGAAAGATCTTGAGGAGGGAGCTTGTAACTCATGCTTTATCCACTGGTTCATCAAAATTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004321 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0008150 GO:0008152 GO:0009058 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0016053 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0032787 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

560

Amino Acids

61.46

Weight (kDa)

6.94

Isoelectric Point (pI)

47.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	57 - 414	4.3e-81	AMP-binding enzyme
AMP-binding_C	PF13193	466 - 541	3e-18	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63380
fragaria_vesca	FvH4_4g24170 FvH4_5g07750 FvH4_5g07750 FvH4_5g07750
malus_domestica	MD06G1147300.v1.1 MD06G1147500.v1.1 MD13G1113700.v1.1 MD14G1161200.v1.1 MD14G1161300.v1.1 MD16G1112900.v1.1 MD16G1113000.v1.1
prunus_persica	Prupe.1G232500_v2.0.a1 Prupe.5G154000_v2.0.a1 Prupe.5G154100_v2.0.a1
pyrus_communis	pycom06g13680 pycom06g13690 pycom13g09900 pycom14g13410 pycom16g09570 pycom16g09580
rosa_chinensis	RchiOBHm_Chr4g0430951 RchiOBHm_Chr7g0190671 RchiOBHm_Chr7g0190681
rosa_laevigata	RLG00000004504 RLG00000006935
rosa_multiflora	Rmu_co8020444.1_g000001 Rmu_sc0006263.1_g000015 Rmu_sc0006709.1_g000003 Rmu_sc0013571.1_g000005
rosa_roxburghii	Rroxscaffold_3G00264410 Rroxscaffold_5G00372640
rosa_rugosa	Rorug04G0247400 Rorug06G0506300 Rorug06G0506400
rosa_samantha	Rh4AG303200 Rh4AG304100 Rh4BG311400 Rh4CG327700 Rh4DG307500 Rh7AG112900 Rh7BG115400 Rh7CG117900 Rh7DG116800
rosa_wichuraiana	Rw4G026360 Rw7G009850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	215
Acc65I	GGTACC	1 cut(s)	1384
AccB1I	GGYRCC	2 cut(s)	623, 1384
AccBSI	CCGCTC	2 cut(s)	51, 112
AccII	CGCG	5 cut(s)	278, 705, 983, 1111, 1469
AccIII	TCCGGA	1 cut(s)	1456
AclWI	GGATC	2 cut(s)	35, 44
AcoI	YGGCCR	4 cut(s)	168, 631, 681, 1134
AcsI	RAATTY	1 cut(s)	521
AcuI	CTGAAG	1 cut(s)	1119
AcvI	CACGTG	2 cut(s)	295, 769
AcyI	GRCGYC	2 cut(s)	198, 363
AdeI	CACNNNGTG	1 cut(s)	877
AfaI	GTAC	8 cut(s)	67, 224, 884, 944, 1044, 1370, 1386, 1565
AfiI	CCNNNNNNNGG	6 cut(s)	50, 94, 481, 867, 1074, 1172
AflIII	ACRYGT	2 cut(s)	768, 1579
AgeI	ACCGGT	1 cut(s)	1298
AgsI	TTSAA	4 cut(s)	286, 581, 1294, 1415
AhdI	GACNNNNNGTC	1 cut(s)	550
AjiI	CACGTC	5 cut(s)	183, 217, 328, 453, 1580
AjnI	CCWGG	3 cut(s)	414, 1131, 1189
AjuI	GAANNNNNNNTTGG	2 cut(s)	914, 946
AluBI	AGCT	6 cut(s)	472, 573, 954, 1207, 1394, 1639
AluI	AGCT	6 cut(s)	472, 573, 954, 1207, 1394, 1639
Alw21I	GWGCWC	1 cut(s)	575
Alw26I	GTCTC	4 cut(s)	223, 806, 1058, 1262
AlwI	GGATC	2 cut(s)	35, 44
Aor13HI	TCCGGA	1 cut(s)	1456
ApeKI	GCWGC	6 cut(s)	17, 602, 754, 1001, 1213, 1439
ApoI	RAATTY	1 cut(s)	521
ArsI	GACNNNNNNTTYG	2 cut(s)	805, 837
AscI	GGCGCGCC	1 cut(s)	981
AsiGI	ACCGGT	1 cut(s)	1298
Asp718I	GGTACC	1 cut(s)	1384
AspLEI	GCGC	5 cut(s)	280, 705, 983, 985, 1111
AspS9I	GGNCC	6 cut(s)	97, 393, 418, 858, 1126, 1220
AsuC2I	CCSGG	4 cut(s)	542, 599, 685, 1506
AsuHPI	GGTGA	6 cut(s)	124, 133, 650, 1028, 1214, 1313
AsuII	TTCGAA	1 cut(s)	843
AvaII	GGWCC	4 cut(s)	97, 393, 418, 1220
BalI	TGGCCA	1 cut(s)	170
BanI	GGYRCC	2 cut(s)	623, 1384
BanII	GRGCYC	2 cut(s)	575, 690
BarI	GAAGNNNNNNTAC	4 cut(s)	38, 70, 215, 247
BbrPI	CACGTG	2 cut(s)	295, 769
BbsI	GAAGAC	2 cut(s)	1301, 1470
Bbv12I	GWGCWC	1 cut(s)	575
BbvCI	CCTCAGC	2 cut(s)	236, 854
BbvI	GCAGC	6 cut(s)	29, 589, 741, 988, 1200, 1426
BccI	CCATC	4 cut(s)	446, 820, 1277, 1428
BceAI	ACGGC	2 cut(s)	618, 845
BcgI	CGANNNNNNTGC	4 cut(s)	584, 618, 652, 686
BciT130I	CCWGG	3 cut(s)	416, 1133, 1191
BcnI	CCSGG	4 cut(s)	542, 599, 685, 1506
BcoDI	GTCTC	4 cut(s)	223, 806, 1058, 1262
BfaI	CTAG	1 cut(s)	1529
BglI	GCCNNNNNGGC	1 cut(s)	680
BglII	AGATCT	1 cut(s)	1623
Bme1390I	CCNGG	7 cut(s)	416, 542, 599, 685, 1133, 1191, 1506
Bme18I	GGWCC	4 cut(s)	97, 393, 418, 1220
BmeRI	GACNNNNNGTC	1 cut(s)	550
BmgBI	CACGTC	5 cut(s)	183, 217, 328, 453, 1580
BmgT120I	GGNCC	6 cut(s)	97, 393, 418, 858, 1126, 1220
BmiI	GGNNCC	6 cut(s)	99, 394, 625, 689, 1331, 1386
BmrFI	CCNGG	7 cut(s)	416, 542, 599, 685, 1133, 1191, 1506
BmsI	GCATC	2 cut(s)	840, 1426
BpiI	GAAGAC	2 cut(s)	1301, 1470
BpmI	CTGGAG	1 cut(s)	1596
Bpu10I	CCTNAGC	2 cut(s)	236, 854
Bpu14I	TTCGAA	1 cut(s)	843
BpuEI	CTTGAG	1 cut(s)	1648
BpuMI	CCSGG	4 cut(s)	542, 599, 685, 1506
Bsa29I	ATCGAT	1 cut(s)	39
BsaAI	YACGTR	3 cut(s)	65, 295, 769
BsaHI	GRCGYC	2 cut(s)	198, 363
BsaI	GGTCTC	3 cut(s)	806, 1058, 1262
BsaJI	CCNNGG	5 cut(s)	165, 816, 861, 1272, 1482
BsaWI	WCCGGW	5 cut(s)	94, 1067, 1172, 1298, 1456
BsaXI	ACNNNNNCTCC	2 cut(s)	188, 218
Bsc4I	CCNNNNNNNGG	6 cut(s)	50, 94, 481, 867, 1074, 1172
Bse118I	RCCGGY	2 cut(s)	629, 1298
Bse1I	ACTGG	3 cut(s)	838, 890, 1666
BseAI	TCCGGA	1 cut(s)	1456
BseBI	CCWGG	3 cut(s)	416, 1133, 1191
BseCI	ATCGAT	1 cut(s)	39
BseDI	CCNNGG	5 cut(s)	165, 816, 861, 1272, 1482
BseGI	GGATG	6 cut(s)	490, 1092, 1282, 1420, 1441, 1465
BseLI	CCNNNNNNNGG	6 cut(s)	50, 94, 481, 867, 1074, 1172
BseMII	CTCAG	9 cut(s)	40, 250, 588, 845, 950, 964, 1257, 1386, 1512
BseNI	ACTGG	3 cut(s)	838, 890, 1666
BsePI	GCGCGC	1 cut(s)	981
BseRI	GAGGAG	5 cut(s)	143, 341, 607, 1086, 1645
BseX3I	CGGCCG	1 cut(s)	631
BseXI	GCAGC	6 cut(s)	29, 589, 741, 988, 1200, 1426
BseYI	CCCAGC	2 cut(s)	988, 1390
Bsh1236I	CGCG	5 cut(s)	278, 705, 983, 1111, 1469
Bsh1285I	CGRYCG	4 cut(s)	211, 390, 541, 634
BshNI	GGYRCC	2 cut(s)	623, 1384
BshTI	ACCGGT	1 cut(s)	1298
BshVI	ATCGAT	1 cut(s)	39
BsiEI	CGRYCG	4 cut(s)	211, 390, 541, 634
BsiHKAI	GWGCWC	1 cut(s)	575
BslFI	GGGAC	4 cut(s)	83, 314, 360, 557
BslI	CCNNNNNNNGG	6 cut(s)	50, 94, 481, 867, 1074, 1172
BsmAI	GTCTC	4 cut(s)	223, 806, 1058, 1262
BsmBI	CGTCTC	1 cut(s)	223
BsmFI	GGGAC	4 cut(s)	83, 314, 360, 557
Bso31I	GGTCTC	3 cut(s)	806, 1058, 1262
Bsp119I	TTCGAA	1 cut(s)	843
Bsp1286I	GDGCHC	2 cut(s)	575, 690
Bsp13I	TCCGGA	1 cut(s)	1456
Bsp1407I	TGTACA	1 cut(s)	1042
Bsp143I	GATC	8 cut(s)	36, 40, 387, 405, 906, 946, 1303, 1623
Bsp19I	CCATGG	2 cut(s)	165, 1482
BspCNI	CTCAG	9 cut(s)	39, 249, 587, 846, 949, 963, 1258, 1387, 1513
BspDI	ATCGAT	1 cut(s)	39
BspEI	TCCGGA	1 cut(s)	1456
BspFNI	CGCG	5 cut(s)	278, 705, 983, 1111, 1469
BspHI	TCATGA	1 cut(s)	1230
BspLI	GGNNCC	6 cut(s)	99, 394, 625, 689, 1331, 1386
BspPI	GGATC	2 cut(s)	35, 44
BspQI	GCTCTTC	1 cut(s)	41
BspT104I	TTCGAA	1 cut(s)	843
BspT107I	GGYRCC	2 cut(s)	623, 1384
BspTNI	GGTCTC	3 cut(s)	806, 1058, 1262
BsrBI	CCGCTC	2 cut(s)	51, 112
BsrFI	RCCGGY	2 cut(s)	629, 1298
BsrGI	TGTACA	1 cut(s)	1042
BsrI	ACTGG	3 cut(s)	838, 890, 1666
BssAI	RCCGGY	2 cut(s)	629, 1298
BssECI	CCNNGG	5 cut(s)	165, 816, 861, 1272, 1482
BssHII	GCGCGC	1 cut(s)	981
BssMI	GATC	8 cut(s)	36, 40, 387, 405, 906, 946, 1303, 1623
BssNI	GRCGYC	2 cut(s)	198, 363
BssT1I	CCWWGG	4 cut(s)	165, 816, 1272, 1482
Bst2UI	CCWGG	3 cut(s)	416, 1133, 1191
Bst4CI	ACNGT	1 cut(s)	502
Bst6I	CTCTTC	1 cut(s)	41
BstACI	GRCGYC	2 cut(s)	198, 363
BstAUI	TGTACA	1 cut(s)	1042
BstBAI	YACGTR	3 cut(s)	65, 295, 769
BstBI	TTCGAA	1 cut(s)	843
BstC8I	GCNNGC	2 cut(s)	956, 983
BstDEI	CTNAG	9 cut(s)	26, 236, 574, 854, 936, 950, 1266, 1395, 1521
BstDSI	CCRYGG	3 cut(s)	165, 861, 1482
BstF5I	GGATG	6 cut(s)	490, 1092, 1282, 1420, 1441, 1465
BstFNI	CGCG	5 cut(s)	278, 705, 983, 1111, 1469
BstHHI	GCGC	5 cut(s)	280, 705, 983, 985, 1111
BstKTI	GATC	8 cut(s)	39, 43, 390, 408, 909, 949, 1306, 1626
BstMAI	GTCTC	4 cut(s)	223, 806, 1058, 1262
BstMBI	GATC	8 cut(s)	36, 40, 387, 405, 906, 946, 1303, 1623
BstMCI	CGRYCG	4 cut(s)	211, 390, 541, 634
BstNI	CCWGG	3 cut(s)	416, 1133, 1191
BstNSI	RCATGY	1 cut(s)	889
BstSCI	CCNGG	7 cut(s)	414, 540, 597, 683, 1131, 1189, 1504
BstUI	CGCG	5 cut(s)	278, 705, 983, 1111, 1469
BstV1I	GCAGC	6 cut(s)	29, 589, 741, 988, 1200, 1426
BstV2I	GAAGAC	2 cut(s)	1301, 1470
BstX2I	RGATCY	1 cut(s)	1623
BstYI	RGATCY	1 cut(s)	1623
BstZI	CGGCCG	1 cut(s)	631
Bsu15I	ATCGAT	1 cut(s)	39
BsuTUI	ATCGAT	1 cut(s)	39
BtgI	CCRYGG	3 cut(s)	165, 861, 1482
BtgZI	GCGATG	3 cut(s)	424, 655, 863
BtrI	CACGTC	5 cut(s)	183, 217, 328, 453, 1580
BtsCI	GGATG	6 cut(s)	490, 1092, 1282, 1420, 1441, 1465
BtsIMutI	CAGTG	3 cut(s)	897, 1518, 1659
Cac8I	GCNNGC	2 cut(s)	956, 983
CciI	TCATGA	1 cut(s)	1230
CfoI	GCGC	5 cut(s)	280, 705, 983, 985, 1111
Cfr10I	RCCGGY	2 cut(s)	629, 1298
Cfr13I	GGNCC	6 cut(s)	97, 393, 418, 858, 1126, 1220
ClaI	ATCGAT	1 cut(s)	39
CseI	GACGC	4 cut(s)	206, 352, 1100, 1475
CsiI	ACCWGGT	1 cut(s)	414
Csp6I	GTAC	8 cut(s)	66, 223, 883, 943, 1043, 1369, 1385, 1564
CspAI	ACCGGT	1 cut(s)	1298
CviAII	CATG	8 cut(s)	166, 532, 784, 886, 1115, 1231, 1483, 1649
CviQI	GTAC	8 cut(s)	66, 223, 883, 943, 1043, 1369, 1385, 1564
DdeI	CTNAG	9 cut(s)	26, 236, 574, 854, 936, 950, 1266, 1395, 1521
DpnI	GATC	8 cut(s)	38, 42, 389, 407, 908, 948, 1305, 1625
DpnII	GATC	8 cut(s)	36, 40, 387, 405, 906, 946, 1303, 1623
DraIII	CACNNNGTG	1 cut(s)	877
DrdI	GACNNNNNNGTC	1 cut(s)	215
DriI	GACNNNNNGTC	1 cut(s)	550
DseDI	GACNNNNNNGTC	1 cut(s)	215
EaeI	YGGCCR	4 cut(s)	168, 631, 681, 1134
EagI	CGGCCG	1 cut(s)	631
Eam1104I	CTCTTC	1 cut(s)	41
Eam1105I	GACNNNNNGTC	1 cut(s)	550
EarI	CTCTTC	1 cut(s)	41
EciI	GGCGGA	2 cut(s)	20, 175
Ecl136II	GAGCTC	1 cut(s)	573
EclXI	CGGCCG	1 cut(s)	631
Eco130I	CCWWGG	4 cut(s)	165, 816, 1272, 1482
Eco147I	AGGCCT	1 cut(s)	1181
Eco24I	GRGCYC	2 cut(s)	575, 690
Eco31I	GGTCTC	3 cut(s)	806, 1058, 1262
Eco47I	GGWCC	4 cut(s)	97, 393, 418, 1220
Eco52I	CGGCCG	1 cut(s)	631
Eco53kI	GAGCTC	1 cut(s)	573
Eco57I	CTGAAG	1 cut(s)	1119
Eco72I	CACGTG	2 cut(s)	295, 769
EcoICRI	GAGCTC	1 cut(s)	573
EcoRI	GAATTC	1 cut(s)	521
EcoRII	CCWGG	3 cut(s)	414, 1131, 1189
EcoT14I	CCWWGG	4 cut(s)	165, 816, 1272, 1482
EcoT38I	GRGCYC	2 cut(s)	575, 690
ErhI	CCWWGG	4 cut(s)	165, 816, 1272, 1482
Esp3I	CGTCTC	1 cut(s)	223
FaeI	CATG	8 cut(s)	169, 535, 787, 889, 1118, 1234, 1486, 1652
FaqI	GGGAC	4 cut(s)	83, 314, 360, 557
FatI	CATG	8 cut(s)	165, 531, 783, 885, 1114, 1230, 1482, 1648
FauI	CCCGC	4 cut(s)	114, 385, 442, 1462
FauNDI	CATATG	1 cut(s)	1489
FokI	GGATG	6 cut(s)	497, 1099, 1289, 1407, 1448, 1472
FriOI	GRGCYC	2 cut(s)	575, 690
FspBI	CTAG	1 cut(s)	1529
GlaI	GCGC	5 cut(s)	279, 704, 982, 984, 1110
GsaI	CCCAGC	2 cut(s)	992, 1394
GsuI	CTGGAG	1 cut(s)	1596
HgaI	GACGC	4 cut(s)	206, 352, 1100, 1475
HhaI	GCGC	5 cut(s)	280, 705, 983, 985, 1111
Hin1I	GRCGYC	2 cut(s)	198, 363
Hin1II	CATG	8 cut(s)	169, 535, 787, 889, 1118, 1234, 1486, 1652
Hin6I	GCGC	5 cut(s)	278, 703, 981, 983, 1109
HinP1I	GCGC	5 cut(s)	278, 703, 981, 983, 1109
HincII	GTYRAC	4 cut(s)	586, 654, 1162, 1345
HindII	GTYRAC	4 cut(s)	586, 654, 1162, 1345
HinfI	GANTC	7 cut(s)	11, 398, 512, 607, 655, 1447, 1477
HphI	GGTGA	6 cut(s)	124, 133, 650, 1028, 1214, 1313
Hpy166II	GTNNAC	5 cut(s)	586, 654, 750, 1162, 1345
Hpy188I	TCNGA	8 cut(s)	29, 45, 208, 387, 397, 403, 928, 939
Hpy188III	TCNNGA	4 cut(s)	1231, 1424, 1457, 1627
Hpy8I	GTNNAC	5 cut(s)	586, 654, 750, 1162, 1345
Hpy99I	CGWCG	6 cut(s)	200, 368, 371, 731, 1430, 1581
HpyAV	CCTTC	8 cut(s)	85, 241, 310, 327, 367, 454, 707, 1228
HpyCH4III	ACNGT	1 cut(s)	502
HpyCH4V	TGCA	1 cut(s)	1547
HpyF3I	CTNAG	9 cut(s)	26, 236, 574, 854, 936, 950, 1266, 1395, 1521
Hsp92I	GRCGYC	2 cut(s)	198, 363
Hsp92II	CATG	8 cut(s)	169, 535, 787, 889, 1118, 1234, 1486, 1652
HspAI	GCGC	5 cut(s)	278, 703, 981, 983, 1109
Kpn2I	TCCGGA	1 cut(s)	1456
KpnI	GGTACC	1 cut(s)	1388
Kzo9I	GATC	8 cut(s)	36, 40, 387, 405, 906, 946, 1303, 1623
LguI	GCTCTTC	1 cut(s)	41
LmnI	GCTCC	5 cut(s)	327, 477, 693, 1564, 1636
Lsp1109I	GCAGC	6 cut(s)	29, 589, 741, 988, 1200, 1426
LweI	GCATC	2 cut(s)	840, 1426
MabI	ACCWGGT	1 cut(s)	414
MaeI	CTAG	1 cut(s)	1529
MaeIII	GTNAC	3 cut(s)	556, 1310, 1642
MalI	GATC	8 cut(s)	38, 42, 389, 407, 908, 948, 1305, 1625
MbiI	CCGCTC	2 cut(s)	51, 112
MboI	GATC	8 cut(s)	36, 40, 387, 405, 906, 946, 1303, 1623
MboII	GAAGA	7 cut(s)	58, 772, 1112, 1292, 1306, 1475, 1582
MflI	RGATCY	1 cut(s)	1623
MhlI	GDGCHC	2 cut(s)	575, 690
MlsI	TGGCCA	1 cut(s)	170
MluCI	AATT	5 cut(s)	137, 521, 664, 1358, 1673
MluNI	TGGCCA	1 cut(s)	170
MlyI	GAGTC	3 cut(s)	407, 506, 649
MmeI	TCCRAC	5 cut(s)	14, 231, 906, 1247, 1380
Mox20I	TGGCCA	1 cut(s)	170
MroI	TCCGGA	1 cut(s)	1456
MscI	TGGCCA	1 cut(s)	170
MseI	TTAA	2 cut(s)	1058, 1352
MslI	CAYNNNNRTG	1 cut(s)	890
Msp20I	TGGCCA	1 cut(s)	170
MspA1I	CMGCKG	2 cut(s)	988, 1394
MspR9I	CCNGG	7 cut(s)	416, 542, 599, 685, 1133, 1191, 1506
MvaI	CCWGG	3 cut(s)	416, 1133, 1191
MvnI	CGCG	5 cut(s)	278, 705, 983, 1111, 1469
NciI	CCSGG	4 cut(s)	542, 599, 685, 1506
NcoI	CCATGG	2 cut(s)	165, 1482
NdeI	CATATG	1 cut(s)	1489
NdeII	GATC	8 cut(s)	36, 40, 387, 405, 906, 946, 1303, 1623
NlaIII	CATG	8 cut(s)	169, 535, 787, 889, 1118, 1234, 1486, 1652
NlaIV	GGNNCC	6 cut(s)	99, 394, 625, 689, 1331, 1386
NmeAIII	GCCGAG	1 cut(s)	948
NspI	RCATGY	1 cut(s)	889
NspV	TTCGAA	1 cut(s)	843
PagI	TCATGA	1 cut(s)	1230
PalAI	GGCGCGCC	1 cut(s)	981
PauI	GCGCGC	1 cut(s)	981
PceI	AGGCCT	1 cut(s)	1181
PciSI	GCTCTTC	1 cut(s)	41
PcsI	WCGNNNNNNNCGW	6 cut(s)	204, 360, 366, 516, 552, 735
PfeI	GAWTC	4 cut(s)	11, 607, 1447, 1477
PinAI	ACCGGT	1 cut(s)	1298
Ple19I	CGATCG	1 cut(s)	390
PleI	GAGTC	3 cut(s)	406, 506, 649
PmaCI	CACGTG	2 cut(s)	295, 769
PmlI	CACGTG	2 cut(s)	295, 769
PpsI	GAGTC	3 cut(s)	406, 506, 649
Ppu21I	YACGTR	3 cut(s)	65, 295, 769
Psp124BI	GAGCTC	1 cut(s)	575
Psp6I	CCWGG	3 cut(s)	414, 1131, 1189
PspCI	CACGTG	2 cut(s)	295, 769
PspFI	CCCAGC	2 cut(s)	988, 1390
PspGI	CCWGG	3 cut(s)	414, 1131, 1189
PspN4I	GGNNCC	6 cut(s)	99, 394, 625, 689, 1331, 1386
PspPI	GGNCC	6 cut(s)	97, 393, 418, 858, 1126, 1220
PsuI	RGATCY	1 cut(s)	1623
PteI	GCGCGC	1 cut(s)	981
PvuI	CGATCG	1 cut(s)	390
PvuII	CAGCTG	1 cut(s)	1394
RsaI	GTAC	8 cut(s)	67, 224, 884, 944, 1044, 1370, 1386, 1565
RsaNI	GTAC	8 cut(s)	66, 223, 883, 943, 1043, 1369, 1385, 1564
RseI	CAYNNNNRTG	1 cut(s)	890
SacI	GAGCTC	1 cut(s)	575
SapI	GCTCTTC	1 cut(s)	41
SaqAI	TTAA	2 cut(s)	1058, 1352
Sau3AI	GATC	8 cut(s)	36, 40, 387, 405, 906, 946, 1303, 1623
Sau96I	GGNCC	6 cut(s)	97, 393, 418, 858, 1126, 1220
SchI	GAGTC	3 cut(s)	407, 506, 649
ScrFI	CCNGG	7 cut(s)	416, 542, 599, 685, 1133, 1191, 1506
SduI	GDGCHC	2 cut(s)	575, 690
SexAI	ACCWGGT	1 cut(s)	414
SfaNI	GCATC	2 cut(s)	840, 1426
SfuI	TTCGAA	1 cut(s)	843
SgsI	GGCGCGCC	1 cut(s)	981
SinI	GGWCC	4 cut(s)	97, 393, 418, 1220
SmiMI	CAYNNNNRTG	1 cut(s)	890
SmlI	CTYRAG	1 cut(s)	1627
SmoI	CTYRAG	1 cut(s)	1627
Sse9I	AATT	5 cut(s)	137, 521, 664, 1358, 1673
SseBI	AGGCCT	1 cut(s)	1181
SspMI	CTAG	1 cut(s)	1529
SstI	GAGCTC	1 cut(s)	575
StuI	AGGCCT	1 cut(s)	1181
StyD4I	CCNGG	7 cut(s)	414, 540, 597, 683, 1131, 1189, 1504
StyI	CCWWGG	4 cut(s)	165, 816, 1272, 1482
TaaI	ACNGT	1 cut(s)	502
TaqI	TCGA	7 cut(s)	39, 195, 510, 537, 726, 843, 920
TasI	AATT	5 cut(s)	137, 521, 664, 1358, 1673
TatI	WGTACW	2 cut(s)	1042, 1368
TauI	GCSGC	6 cut(s)	54, 112, 203, 981, 988, 1131
TfiI	GAWTC	4 cut(s)	11, 607, 1447, 1477
Tru1I	TTAA	2 cut(s)	1058, 1352
Tru9I	TTAA	2 cut(s)	1058, 1352
TscAI	CASTG	3 cut(s)	897, 1525, 1666
TseI	GCWGC	6 cut(s)	17, 602, 754, 1001, 1213, 1439
TspDTI	ATGAA	7 cut(s)	292, 658, 772, 1247, 1293, 1476, 1656
TspGWI	ACGGA	3 cut(s)	146, 544, 1551
TspRI	CASTG	3 cut(s)	897, 1525, 1666
VpaK11BI	GGWCC	4 cut(s)	97, 393, 418, 1220
XapI	RAATTY	1 cut(s)	521
XceI	RCATGY	1 cut(s)	889
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1489
XspI	CTAG	1 cut(s)	1529

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.