Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0001900

Physical Location & Seq

Genomic Coordinates Reverse (-)

52505015 .. 52507477

Gene Structure 2463 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G026360.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGGCCGATCACAACACTACCAGCATTGATCCCAACAGCGGCTTCTGCTCATACACCAAAACATTCCACAGTCTCAGGCCGAAAGCCCCGCTACCCTCCCAAACGACGCCGCTTTCGATCACTCACTACATTTTCTCCCACCTCCAAGCCTCCCCTCCTCCGCCCTCCACCCCGGCTCTCCTCGACCCCGCCACGCGCCGCCACATTCTCTACCCCGAGTTCACGCGCCGCGTCAAAACCCTAGCAGCCGCGCTAGAATCGCAACTCAACCTCTCCAATGGCCACACCGCCTTCGTCATCTCCCCCAATTCTCTCGACCTCCCCATCCTCTACTTCTCTCTCTTTTCCCTCGGCGTAACCGTCTCTCCGTCAAACCCGGCCAGCACCACTCCCGAGATTTCCCGCCAAATCCACCTCAGCAGACCCGTCATCGCCTTCGCCACCTCCGCCACCGCTCACAAACTCCCCAACTCCCTCCAATACGGCGTTGTACTCCTCGACTCCCCGGAATTCCAGTCCATGATGACGTGTGCTCCGGCGCCGGAGCTTCCACGGGTCCGCTTCAGCCAGTCCCACACCGCCGCGGTCCTCTACTCGTCGGGGACCACCGGGGCCGTCAAAGGCGTGGAGTTGACCCACCGTAACTGGATTTCCGTGCTGGCGTCGGCGTTCGCGGTTCGGAATCCGACGGCTCCGCCAGCTGTCATGCTCTGCGCGGTGCCGTTCTTCCACGTGTACGGCTTCGGCGTCTGCATGCGGGTGTTGGGGTTCGGCGAGACGCTGGCGTCGATTTCGGGGAGGTTTGATCTGAGAGCGACGCTGAGGGCCGTCGAGGAGTTCAGGATCAGCCACGTATCTTGGGCCCCGCCGGTGGTGGTGGCGGTGGTGAAGCTCGGGAGCGAGATCGACGGCTACGATCTGAGCTCGCTGCAGGTCGTCGCCTGCGGCGGTGCTCCGCTTGCGAAGAGTGTCATCGATAAGCTGAAGAAACGCCTTCCTAACGTGCAAGTCGCACAGGGATATGGGCTGACAGAGACAACGGGTCGAGTGTTTGGCACGGTAGGCCCAAATGAGACCCGAGTAGAAGGAGCTACTGGAAAGCTGATGTCAAACTTTGAAGCCAAGATTGTTGACCCAGAGACCGGCAGTGCCTTGCCTCCTCTAATGACTGGAGAGCTTTGGCTTAGGGGACCAATTATAATGAAAGGTTACATTGGTGATGAAGAAGCAAATGCTATAACTATGGACACTGAAGGATGGTTTAAAACTGGAGACCTTTGCTATATCGACAAAGAAGGTTACCTATTTTTCGTTGATCGGATCAAGGAACTGATCAAGTACAAAGGCTACCAGGTTGCCCCGGCAGAGTTGGAGCATCTGCTTCAATCCCATCCCGATATTGCTGATGCAGCTGTGGTTCCGTATCCTGATGAGGAGGCAGGTCAAGTACCAATGGCCTTTGTGGTCAGGCGCCTAGGAAGTGTCGTCGATGAATCGCAGGTGAAGGATTTTATTGCCAAACAGGTCGCACCGTATAAGAAAATACGACGAGTAGCATTCATCAATGAAGTACCAAAGAGCGCACAAGGTAAATTAATGAGGAAGGAATTAGTCAAACTTGCATTATCCAAGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004321 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0008150 GO:0008152 GO:0009058 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0016053 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0032787 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

545

Amino Acids

59.29

Weight (kDa)

8.7

Isoelectric Point (pI)

43.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	55 - 404	2.7e-70	AMP-binding enzyme
AMP-binding_C	PF13193	456 - 531	1.1e-17	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63380
fragaria_vesca	FvH4_4g24170 FvH4_5g07750 FvH4_5g07750 FvH4_5g07750
malus_domestica	MD06G1147300.v1.1 MD06G1147500.v1.1 MD13G1113700.v1.1 MD14G1161200.v1.1 MD14G1161300.v1.1 MD16G1112900.v1.1 MD16G1113000.v1.1
prunus_persica	Prupe.1G232500_v2.0.a1 Prupe.5G154000_v2.0.a1 Prupe.5G154100_v2.0.a1
pyrus_communis	pycom06g13680 pycom06g13690 pycom13g09900 pycom14g13410 pycom16g09570 pycom16g09580
rosa_chinensis	RchiOBHm_Chr4g0430951 RchiOBHm_Chr7g0190671 RchiOBHm_Chr7g0190681
rosa_laevigata	RLG00000004504 RLG00000006935
rosa_multiflora	Rmu_co8020444.1_g000001 Rmu_sc0006263.1_g000015 Rmu_sc0006709.1_g000003 Rmu_sc0013571.1_g000005
rosa_roxburghii	Rroxscaffold_3G00264410 Rroxscaffold_5G00372640
rosa_rugosa	Rorug04G0247400 Rorug06G0506300 Rorug06G0506400
rosa_samantha	Rh4AG303200 Rh4AG304100 Rh4BG311400 Rh4CG327700 Rh4DG307500 Rh7AG112900 Rh7BG115400 Rh7CG117900 Rh7DG116800
rosa_wichuraiana	Rw4G026360 Rw7G009850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1199
AarI	CACCTGC	1 cut(s)	1489
AasI	GACNNNNNNGTC	1 cut(s)	784
Acc36I	ACCTGC	3 cut(s)	922, 1430, 1489
AccB1I	GGYRCC	3 cut(s)	538, 718, 1470
AccBSI	CCGCTC	1 cut(s)	455
AccII	CGCG	7 cut(s)	196, 226, 231, 251, 584, 674, 716
AclWI	GGATC	3 cut(s)	23, 851, 1328
AcoI	YGGCCR	3 cut(s)	3, 280, 378
AcsI	RAATTY	1 cut(s)	509
AcuI	CTGAAG	3 cut(s)	547, 1004, 1272
AcvI	CACGTG	1 cut(s)	733
AcyI	GRCGYC	6 cut(s)	107, 539, 662, 747, 785, 1471
AfaI	GTAC	5 cut(s)	492, 737, 1340, 1449, 1572
AfiI	CCNNNNNNNGG	3 cut(s)	38, 871, 1142
AflIII	ACRYGT	2 cut(s)	527, 732
AgsI	TTSAA	2 cut(s)	1118, 1385
AhdI	GACNNNNNGTC	1 cut(s)	1041
AjiI	CACGTC	1 cut(s)	528
AjnI	CCWGG	1 cut(s)	1350
AjuI	GAANNNNNNNTTGG	2 cut(s)	26, 58
AluBI	AGCT	9 cut(s)	547, 701, 892, 924, 982, 1091, 1102, 1177, 1412
AluI	AGCT	9 cut(s)	547, 701, 892, 924, 982, 1091, 1102, 1177, 1412
Alw21I	GWGCWC	3 cut(s)	535, 926, 955
Alw26I	GTCTC	7 cut(s)	77, 367, 770, 1028, 1067, 1133, 1266
AlwI	GGATC	3 cut(s)	23, 851, 1328
Ama87I	CYCGRG	4 cut(s)	215, 392, 893, 1077
AoxI	GGCC	9 cut(s)	3, 77, 280, 378, 612, 825, 861, 1063, 1455
ApaI	GGGCCC	1 cut(s)	865
ApeKI	GCWGC	3 cut(s)	245, 928, 1409
ApoI	RAATTY	1 cut(s)	509
ArsI	GACNNNNNNTTYG	2 cut(s)	97, 129
AseI	ATTAAT	1 cut(s)	1595
AspA2I	CCTAGG	1 cut(s)	1474
AspLEI	GCGC	7 cut(s)	198, 228, 253, 541, 716, 1473, 1583
AspS9I	GGNCC	9 cut(s)	556, 586, 603, 612, 825, 861, 862, 1064, 1190
AsuC2I	CCSGG	5 cut(s)	173, 377, 506, 610, 1361
AsuHPI	GGTGA	3 cut(s)	898, 1229, 1513
AvaI	CYCGRG	4 cut(s)	215, 392, 893, 1077
AvaII	GGWCC	4 cut(s)	556, 586, 603, 1190
AvrII	CCTAGG	1 cut(s)	1474
BaeGI	GKGCMC	1 cut(s)	865
BalI	TGGCCA	1 cut(s)	282
BanI	GGYRCC	3 cut(s)	538, 718, 1470
BanII	GRGCYC	2 cut(s)	865, 926
BbrPI	CACGTG	1 cut(s)	733
Bbv12I	GWGCWC	3 cut(s)	535, 926, 955
BbvCI	CCTCAGC	2 cut(s)	416, 821
BbvI	GCAGC	3 cut(s)	257, 915, 1421
BccI	CCATC	3 cut(s)	332, 1251, 1398
BceAI	ACGGC	7 cut(s)	499, 599, 705, 706, 754, 812, 925
BciT130I	CCWGG	1 cut(s)	1352
BciVI	GTATCC	1 cut(s)	1434
BclI	TGATCA	1 cut(s)	1332
BcnI	CCSGG	5 cut(s)	173, 377, 506, 610, 1361
BcoDI	GTCTC	7 cut(s)	77, 367, 770, 1028, 1067, 1133, 1266
BfaI	CTAG	3 cut(s)	242, 254, 1475
BfmI	CTRYAG	1 cut(s)	929
BfoI	RGCGCY	2 cut(s)	542, 1474
BfuAI	ACCTGC	3 cut(s)	922, 1430, 1489
BfuI	GTATCC	1 cut(s)	1434
BlnI	CCTAGG	1 cut(s)	1474
Bme1390I	CCNGG	6 cut(s)	173, 377, 506, 610, 1352, 1361
Bme18I	GGWCC	4 cut(s)	556, 586, 603, 1190
BmeRI	GACNNNNNGTC	1 cut(s)	1041
BmeT110I	CYCGRG	4 cut(s)	215, 392, 893, 1077
BmgBI	CACGTC	1 cut(s)	528
BmgT120I	GGNCC	9 cut(s)	556, 586, 603, 612, 825, 861, 862, 1064, 1190
BmrFI	CCNGG	6 cut(s)	173, 377, 506, 610, 1352, 1361
BmsI	GCATC	2 cut(s)	1384, 1396
BpmI	CTGGAG	2 cut(s)	1191, 1290
Bpu10I	CCTNAGC	3 cut(s)	416, 821, 1184
BpuMI	CCSGG	5 cut(s)	173, 377, 506, 610, 1361
Bsa29I	ATCGAT	1 cut(s)	975
BsaAI	YACGTR	2 cut(s)	733, 853
BsaHI	GRCGYC	6 cut(s)	107, 539, 662, 747, 785, 1471
BsaI	GGTCTC	3 cut(s)	1067, 1133, 1266
BsaJI	CCNNGG	8 cut(s)	171, 349, 504, 551, 582, 609, 1359, 1474
BsaXI	ACNNNNNCTCC	6 cut(s)	119, 149, 349, 379, 517, 547
Bsc4I	CCNNNNNNNGG	3 cut(s)	38, 871, 1142
Bse118I	RCCGGY	2 cut(s)	868, 1142
Bse1I	ACTGG	6 cut(s)	514, 568, 650, 1099, 1174, 1273
BseBI	CCWGG	1 cut(s)	1352
BseCI	ATCGAT	1 cut(s)	975
BseDI	CCNNGG	8 cut(s)	171, 349, 504, 551, 582, 609, 1359, 1474
BseGI	GGATG	3 cut(s)	324, 1262, 1390
BseLI	CCNNNNNNNGG	3 cut(s)	38, 871, 1142
BseMII	CTCAG	5 cut(s)	88, 430, 800, 812, 911
BseNI	ACTGG	6 cut(s)	514, 568, 650, 1099, 1174, 1273
BseRI	GAGGAG	6 cut(s)	147, 170, 485, 848, 1149, 1448
BseSI	GKGCMC	1 cut(s)	865
BseXI	GCAGC	3 cut(s)	257, 915, 1421
Bsh1236I	CGCG	7 cut(s)	196, 226, 231, 251, 584, 674, 716
BshFI	GGCC	9 cut(s)	5, 79, 282, 380, 614, 827, 863, 1065, 1457
BshNI	GGYRCC	3 cut(s)	538, 718, 1470
BshVI	ATCGAT	1 cut(s)	975
BsiHKAI	GWGCWC	3 cut(s)	535, 926, 955
BsiHKCI	CYCGRG	4 cut(s)	215, 392, 893, 1077
BsiSI	CCGG	9 cut(s)	173, 377, 506, 536, 542, 609, 869, 1143, 1361
BslFI	GGGAC	3 cut(s)	556, 616, 1203
BslI	CCNNNNNNNGG	3 cut(s)	38, 871, 1142
BsmAI	GTCTC	7 cut(s)	77, 367, 770, 1028, 1067, 1133, 1266
BsmBI	CGTCTC	2 cut(s)	367, 770
BsmFI	GGGAC	3 cut(s)	556, 616, 1203
BsmI	GAATGC	1 cut(s)	1556
BsnI	GGCC	9 cut(s)	5, 79, 282, 380, 614, 827, 863, 1065, 1457
Bso31I	GGTCTC	3 cut(s)	1067, 1133, 1266
BsoBI	CYCGRG	4 cut(s)	215, 392, 893, 1077
Bsp120I	GGGCCC	1 cut(s)	861
Bsp1286I	GDGCHC	4 cut(s)	535, 865, 926, 955
BspANI	GGCC	9 cut(s)	5, 79, 282, 380, 614, 827, 863, 1065, 1457
BspCNI	CTCAG	5 cut(s)	87, 429, 801, 813, 912
BspDI	ATCGAT	1 cut(s)	975
BspFNI	CGCG	7 cut(s)	196, 226, 231, 251, 584, 674, 716
BspMAI	CTGCAG	1 cut(s)	933
BspMI	ACCTGC	3 cut(s)	922, 1430, 1489
BspPI	GGATC	3 cut(s)	23, 851, 1328
BspT107I	GGYRCC	3 cut(s)	538, 718, 1470
BspTNI	GGTCTC	3 cut(s)	1067, 1133, 1266
BsrBI	CCGCTC	1 cut(s)	455
BsrFI	RCCGGY	2 cut(s)	868, 1142
BsrI	ACTGG	6 cut(s)	514, 568, 650, 1099, 1174, 1273
BssAI	RCCGGY	2 cut(s)	868, 1142
BssECI	CCNNGG	8 cut(s)	171, 349, 504, 551, 582, 609, 1359, 1474
BssNI	GRCGYC	6 cut(s)	107, 539, 662, 747, 785, 1471
BssT1I	CCWWGG	1 cut(s)	1474
Bst2UI	CCWGG	1 cut(s)	1352
Bst4CI	ACNGT	5 cut(s)	71, 361, 641, 1060, 1533
Bst6I	CTCTTC	1 cut(s)	959
BstACI	GRCGYC	6 cut(s)	107, 539, 662, 747, 785, 1471
BstBAI	YACGTR	2 cut(s)	733, 853
BstC8I	GCNNGC	8 cut(s)	382, 660, 699, 755, 783, 926, 943, 960
BstDEI	CTNAG	6 cut(s)	74, 416, 809, 821, 920, 1184
BstDSI	CCRYGG	2 cut(s)	551, 582
BstEII	GGTNACC	1 cut(s)	1298
BstF5I	GGATG	3 cut(s)	324, 1262, 1390
BstFNI	CGCG	7 cut(s)	196, 226, 231, 251, 584, 674, 716
BstH2I	RGCGCY	2 cut(s)	542, 1474
BstHHI	GCGC	7 cut(s)	198, 228, 253, 541, 716, 1473, 1583
BstMAI	GTCTC	7 cut(s)	77, 367, 770, 1028, 1067, 1133, 1266
BstMWI	GCNNNNNNNGC	7 cut(s)	45, 259, 446, 698, 959, 1062, 1409
BstNI	CCWGG	1 cut(s)	1352
BstNSI	RCATGY	1 cut(s)	757
BstPI	GGTNACC	1 cut(s)	1298
BstSCI	CCNGG	6 cut(s)	171, 375, 504, 608, 1350, 1359
BstSFI	CTRYAG	1 cut(s)	929
BstSLI	GKGCMC	1 cut(s)	865
BstUI	CGCG	7 cut(s)	196, 226, 231, 251, 584, 674, 716
BstV1I	GCAGC	3 cut(s)	257, 915, 1421
Bsu15I	ATCGAT	1 cut(s)	975
BsuI	GTATCC	1 cut(s)	1434
BsuRI	GGCC	9 cut(s)	5, 79, 282, 380, 614, 827, 863, 1065, 1457
BsuTUI	ATCGAT	1 cut(s)	975
BtgI	CCRYGG	2 cut(s)	551, 582
BtgZI	GCGATG	1 cut(s)	415
BtrI	CACGTC	1 cut(s)	528
BtsCI	GGATG	3 cut(s)	324, 1262, 1390
BtsI	GCAGTG	1 cut(s)	1153
BtsIMutI	CAGTG	2 cut(s)	1153, 1248
BveI	ACCTGC	3 cut(s)	922, 1430, 1489
Cac8I	GCNNGC	8 cut(s)	382, 660, 699, 755, 783, 926, 943, 960
CfoI	GCGC	7 cut(s)	198, 228, 253, 541, 716, 1473, 1583
Cfr10I	RCCGGY	2 cut(s)	868, 1142
Cfr13I	GGNCC	9 cut(s)	556, 586, 603, 612, 825, 861, 862, 1064, 1190
Cfr42I	CCGCGG	1 cut(s)	585
ClaI	ATCGAT	1 cut(s)	975
CseI	GACGC	7 cut(s)	115, 220, 651, 736, 774, 787, 826
CsiI	ACCWGGT	1 cut(s)	1350
Csp6I	GTAC	5 cut(s)	491, 736, 1339, 1448, 1571
CspCI	CAANNNNNGTGG	2 cut(s)	839, 874
CviAII	CATG	3 cut(s)	520, 706, 754
CviQI	GTAC	5 cut(s)	491, 736, 1339, 1448, 1571
DdeI	CTNAG	6 cut(s)	74, 416, 809, 821, 920, 1184
DinI	GGCGCC	2 cut(s)	540, 1472
DraI	TTTAAA	1 cut(s)	1264
DrdI	GACNNNNNNGTC	1 cut(s)	784
DriI	GACNNNNNGTC	1 cut(s)	1041
DseDI	GACNNNNNNGTC	1 cut(s)	784
EaeI	YGGCCR	3 cut(s)	3, 280, 378
Eam1104I	CTCTTC	1 cut(s)	959
Eam1105I	GACNNNNNGTC	1 cut(s)	1041
EarI	CTCTTC	1 cut(s)	959
EciI	GGCGGA	3 cut(s)	150, 436, 684
Ecl136II	GAGCTC	1 cut(s)	924
Eco130I	CCWWGG	1 cut(s)	1474
Eco24I	GRGCYC	2 cut(s)	865, 926
Eco31I	GGTCTC	3 cut(s)	1067, 1133, 1266
Eco47I	GGWCC	4 cut(s)	556, 586, 603, 1190
Eco53kI	GAGCTC	1 cut(s)	924
Eco57I	CTGAAG	3 cut(s)	547, 1004, 1272
Eco72I	CACGTG	1 cut(s)	733
Eco88I	CYCGRG	4 cut(s)	215, 392, 893, 1077
Eco91I	GGTNACC	1 cut(s)	1298
EcoICRI	GAGCTC	1 cut(s)	924
EcoO109I	RGGNCCY	1 cut(s)	862
EcoO65I	GGTNACC	1 cut(s)	1298
EcoRI	GAATTC	1 cut(s)	509
EcoRII	CCWGG	1 cut(s)	1350
EcoT14I	CCWWGG	1 cut(s)	1474
EcoT38I	GRGCYC	2 cut(s)	865, 926
EgeI	GGCGCC	2 cut(s)	540, 1472
EheI	GGCGCC	2 cut(s)	540, 1472
ErhI	CCWWGG	1 cut(s)	1474
Esp3I	CGTCTC	2 cut(s)	367, 770
FaeI	CATG	3 cut(s)	523, 709, 757
FaqI	GGGAC	3 cut(s)	556, 616, 1203
FatI	CATG	3 cut(s)	519, 705, 753
FauI	CCCGC	5 cut(s)	96, 196, 410, 750, 873
FbaI	TGATCA	1 cut(s)	1332
FokI	GGATG	3 cut(s)	311, 1269, 1377
FriOI	GRGCYC	2 cut(s)	865, 926
FspBI	CTAG	3 cut(s)	242, 254, 1475
GlaI	GCGC	7 cut(s)	197, 227, 252, 540, 715, 1472, 1582
GsuI	CTGGAG	2 cut(s)	1191, 1290
HaeII	RGCGCY	2 cut(s)	542, 1474
HaeIII	GGCC	9 cut(s)	5, 79, 282, 380, 614, 827, 863, 1065, 1457
HapII	CCGG	9 cut(s)	173, 377, 506, 536, 542, 609, 869, 1143, 1361
HgaI	GACGC	7 cut(s)	115, 220, 651, 736, 774, 787, 826
HhaI	GCGC	7 cut(s)	198, 228, 253, 541, 716, 1473, 1583
Hin1I	GRCGYC	6 cut(s)	107, 539, 662, 747, 785, 1471
Hin1II	CATG	3 cut(s)	523, 709, 757
Hin6I	GCGC	7 cut(s)	196, 226, 251, 539, 714, 1471, 1581
HinP1I	GCGC	7 cut(s)	196, 226, 251, 539, 714, 1471, 1581
HincII	GTYRAC	2 cut(s)	633, 1132
HindII	GTYRAC	2 cut(s)	633, 1132
HinfI	GANTC	4 cut(s)	257, 500, 682, 1493
HpaII	CCGG	9 cut(s)	173, 377, 506, 536, 542, 609, 869, 1143, 1361
HphI	GGTGA	3 cut(s)	898, 1229, 1513
Hpy166II	GTNNAC	4 cut(s)	222, 633, 736, 1132
Hpy188I	TCNGA	5 cut(s)	681, 687, 810, 921, 1320
Hpy188III	TCNNGA	6 cut(s)	314, 392, 841, 895, 1394, 1427
Hpy8I	GTNNAC	4 cut(s)	222, 633, 736, 1132
HpyAV	CCTTC	8 cut(s)	301, 445, 1004, 1079, 1247, 1289, 1498, 1597
HpyCH4III	ACNGT	5 cut(s)	71, 361, 641, 1060, 1533
HpyCH4IV	ACGT	4 cut(s)	527, 732, 852, 1002
HpyCH4V	TGCA	5 cut(s)	753, 931, 1006, 1409, 1622
HpyF10VI	GCNNNNNNNGC	7 cut(s)	45, 259, 446, 698, 959, 1062, 1409
HpyF3I	CTNAG	6 cut(s)	74, 416, 809, 821, 920, 1184
HpySE526I	ACGT	4 cut(s)	527, 732, 852, 1002
Hsp92I	GRCGYC	6 cut(s)	107, 539, 662, 747, 785, 1471
Hsp92II	CATG	3 cut(s)	523, 709, 757
HspAI	GCGC	7 cut(s)	196, 226, 251, 539, 714, 1471, 1581
KasI	GGCGCC	2 cut(s)	538, 1470
Ksp22I	TGATCA	1 cut(s)	1332
KspI	CCGCGG	1 cut(s)	585
LmnI	GCTCC	7 cut(s)	538, 544, 697, 897, 958, 1088, 1372
Lsp1109I	GCAGC	3 cut(s)	257, 915, 1421
LweI	GCATC	2 cut(s)	1384, 1396
MabI	ACCWGGT	1 cut(s)	1350
MaeI	CTAG	3 cut(s)	242, 254, 1475
MaeII	ACGT	4 cut(s)	527, 732, 852, 1002
MaeIII	GTNAC	4 cut(s)	355, 641, 1208, 1298
MbiI	CCGCTC	1 cut(s)	455
MboII	GAAGA	4 cut(s)	718, 976, 997, 1235
MhlI	GDGCHC	4 cut(s)	535, 865, 926, 955
MlsI	TGGCCA	1 cut(s)	282
MluCI	AATT	5 cut(s)	307, 509, 1194, 1592, 1607
MluNI	TGGCCA	1 cut(s)	282
Mly113I	GGCGCC	2 cut(s)	539, 1471
MlyI	GAGTC	1 cut(s)	494
MmeI	TCCRAC	2 cut(s)	710, 1350
Mox20I	TGGCCA	1 cut(s)	282
MscI	TGGCCA	1 cut(s)	282
MseI	TTAA	2 cut(s)	1263, 1595
MslI	CAYNNNNRTG	1 cut(s)	758
Msp20I	TGGCCA	1 cut(s)	282
MspA1I	CMGCKG	4 cut(s)	39, 584, 701, 1412
MspI	CCGG	9 cut(s)	173, 377, 506, 536, 542, 609, 869, 1143, 1361
MspR9I	CCNGG	6 cut(s)	173, 377, 506, 610, 1352, 1361
Mva1269I	GAATGC	1 cut(s)	1556
MvaI	CCWGG	1 cut(s)	1352
MvnI	CGCG	7 cut(s)	196, 226, 231, 251, 584, 674, 716
MwoI	GCNNNNNNNGC	7 cut(s)	45, 259, 446, 698, 959, 1062, 1409
NarI	GGCGCC	2 cut(s)	539, 1471
NciI	CCSGG	5 cut(s)	173, 377, 506, 610, 1361
NlaIII	CATG	3 cut(s)	523, 709, 757
NmeAIII	GCCGAG	1 cut(s)	330
NspI	RCATGY	1 cut(s)	757
PaeI	GCATGC	1 cut(s)	757
PaqCI	CACCTGC	1 cut(s)	1489
PcsI	WCGNNNNNNNCGW	5 cut(s)	113, 357, 744, 785, 912
PctI	GAATGC	1 cut(s)	1556
PfeI	GAWTC	3 cut(s)	257, 682, 1493
PleI	GAGTC	1 cut(s)	494
PluTI	GGCGCC	2 cut(s)	542, 1474
PmaCI	CACGTG	1 cut(s)	733
PmlI	CACGTG	1 cut(s)	733
PpsI	GAGTC	1 cut(s)	494
Ppu21I	YACGTR	2 cut(s)	733, 853
PshBI	ATTAAT	1 cut(s)	1595
PsiI	TTATAA	1 cut(s)	1199
Psp124BI	GAGCTC	1 cut(s)	926
Psp6I	CCWGG	1 cut(s)	1350
PspCI	CACGTG	1 cut(s)	733
PspEI	GGTNACC	1 cut(s)	1298
PspGI	CCWGG	1 cut(s)	1350
PspOMI	GGGCCC	1 cut(s)	861
PspPI	GGNCC	9 cut(s)	556, 586, 603, 612, 825, 861, 862, 1064, 1190
PstI	CTGCAG	1 cut(s)	933
PvuII	CAGCTG	2 cut(s)	701, 1412
RsaI	GTAC	5 cut(s)	492, 737, 1340, 1449, 1572
RsaNI	GTAC	5 cut(s)	491, 736, 1339, 1448, 1571
RseI	CAYNNNNRTG	1 cut(s)	758
SacI	GAGCTC	1 cut(s)	926
SacII	CCGCGG	1 cut(s)	585
SaqAI	TTAA	2 cut(s)	1263, 1595
Sau96I	GGNCC	9 cut(s)	556, 586, 603, 612, 825, 861, 862, 1064, 1190
SchI	GAGTC	1 cut(s)	494
ScrFI	CCNGG	6 cut(s)	173, 377, 506, 610, 1352, 1361
SduI	GDGCHC	4 cut(s)	535, 865, 926, 955
SexAI	ACCWGGT	1 cut(s)	1350
SfaNI	GCATC	2 cut(s)	1384, 1396
SfcI	CTRYAG	1 cut(s)	929
SfoI	GGCGCC	2 cut(s)	540, 1472
Sfr303I	CCGCGG	1 cut(s)	585
SgrAI	CRCCGGYG	1 cut(s)	868
SgrBI	CCGCGG	1 cut(s)	585
SinI	GGWCC	4 cut(s)	556, 586, 603, 1190
SmiMI	CAYNNNNRTG	1 cut(s)	758
SphI	GCATGC	1 cut(s)	757
Sse9I	AATT	5 cut(s)	307, 509, 1194, 1592, 1607
SspDI	GGCGCC	2 cut(s)	538, 1470
SspMI	CTAG	3 cut(s)	242, 254, 1475
SstI	GAGCTC	1 cut(s)	926
StyD4I	CCNGG	6 cut(s)	171, 375, 504, 608, 1350, 1359
StyI	CCWWGG	1 cut(s)	1474
TaaI	ACNGT	5 cut(s)	71, 361, 641, 1060, 1533
TaiI	ACGT	4 cut(s)	530, 735, 855, 1005
TasI	AATT	5 cut(s)	307, 509, 1194, 1592, 1607
TatI	WGTACW	2 cut(s)	490, 1338
TauI	GCSGC	7 cut(s)	42, 112, 201, 231, 251, 584, 948
TfiI	GAWTC	3 cut(s)	257, 682, 1493
Tru1I	TTAA	2 cut(s)	1263, 1595
Tru9I	TTAA	2 cut(s)	1263, 1595
TscAI	CASTG	2 cut(s)	1153, 1255
TseI	GCWGC	3 cut(s)	245, 928, 1409
TspDTI	ATGAA	5 cut(s)	1217, 1236, 1506, 1549, 1581
TspGWI	ACGGA	3 cut(s)	357, 643, 1410
TspRI	CASTG	2 cut(s)	1153, 1255
VpaK11BI	GGWCC	4 cut(s)	556, 586, 603, 1190
VspI	ATTAAT	1 cut(s)	1595
XapI	RAATTY	1 cut(s)	509
XceI	RCATGY	1 cut(s)	757
XmaJI	CCTAGG	1 cut(s)	1474
XspI	CTAG	3 cut(s)	242, 254, 1475

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.