Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001900

Physical Location & Seq

Genomic Coordinates Forward (+)

7897278 .. 7900124

Gene Structure 2847 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1112900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1647 bp

ATGGCGACTCAACAGAAGGGCAGTAGCATTGATCCAAAGAGTGGGTTTTGCTCAAAGACCAAGACTTTCCACAGCCTGAGACCCAAATCCCAACTCCCTCCCCAAACCACGCCTCTTTCCCTCACCCACTACCTTCTTTCCCACCTCCACCATTCCCCTCCCCTGCCTTCCACTCCCGCCCTCCTCGACGCCGCCACCGGCCACCGTATTCTCTACCCCGAGTTCATTTTTCGAGTCCGAACCCTCGCCGCCTCGCTTCAATCCCAGCTCGGCCTCTCCCACGGCCAATGTGCCTTGGTTCTTTCCCCCAACTCCCTCCACCTCCCCATCCTCCACCTCTCTCTCCTCTCCCTCGGTGTCATCGTCTCCCCTTCCAACCCCGCCAGCTCCAGTCCCGAAATTTCCCGCCAAATCCGCATATGCAAGCCCGCCGTCGCATTCGCCACCTCCGCCACCGCTCAAAAAATTCCCAACTCCCTCCGCCTCGGGACCATCCTCCTTGACTCGGCCGAGTTCGAGTCCATGATGATGACATGTCCGAGTTCGACCGAATCGCTTCGAGTTGAAGTGAAGCAATCGGACACCGCCACGATTCTCTACTCATCCGGGACCACCGGGAGGGTCAAAGGTGTGGAGTTGACTCACCGGAACTGGATATCGATGATGGCCGGCATCCACGCGGTTCGAAACCGGTCGGCTCCACACGCTGTGTGTCTCTGTACGGTGCCATTTTTCCACGTGTACGGGTTCGCGTACTGCCTGAGAGTTTTGGTGACGGGGGACACACTGGCGTGGATGGGAAGGTTCGATTTAAAGGTGATGATGAGATCCATCGAGCGGTTCCGAATCGCCCACATGGCCTGTGCCCCGCCGGCGGTCGTGGCCTTGGTGAAGCACGGAGACATAAACGAGATGGACGGCTACGATTTGAGCTCCCTGCAAGTGATCGGCTGCGGCGGCGCTCCGCTGTCGAAGAGTGTGGTCGAGAAGCTGAGGAAGCGGCTGCCCAACGTACAAGTCTCACAGGGATATGGGATGACGGAGACGACGGCCCGAGTGTTTGGCGCGCTGGGCCCGGAAGAAACCCGAGTGGAAGGAGCTAATGGAAGGCTGATGTCAGATATTGAGGCCAAGATTGTGGAGACTGAGACTGGCAGTGCCCTGCCTCCTTTGATGCATGGGGAAATTTGGGTTAGAGGACCATACGTTATGAAAGGTTACACTGGTGACGAAGAAGCAACTGCTGCAATTTTCGACTCAGAAGGATGGTTAAAAACTGGAGACATTTGCTACATAGACAATGAAGGTTTTCTGTATTTCGTCGACCGAATCAAGGAATTGATCAAGTACAAAGGCTACCAGGTTGCCCCGGCGGAGTTGGAGGATTTACTTCATTCCCATCCAGACATTGTCGATGCAGCTGTGATTCCGTACCCAGATGAGGAAGCAGGTCAAGTACCCATGGCCTTCGTCGTGAGGAGCCTTGGAAGTGCCATTGATGAATCACAGATCACGGATTTCATTGCAAAACAGGTTGCGCCATATAAGAAGATTCGACGCGTAGCATTCATCAGTGCAATACCAAAGAATGTGCAGGGAAAAGTGTTGAGGAAGGAATTAATCAAACTTGCACTATCCAAGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004321 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0008150 GO:0008152 GO:0009058 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0016053 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0032787 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

549

Amino Acids

59.84

Weight (kDa)

8.66

Isoelectric Point (pI)

44.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	56 - 407	1.5e-77	AMP-binding enzyme
AMP-binding_C	PF13193	459 - 534	2e-16	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63380
fragaria_vesca	FvH4_4g24170 FvH4_5g07750 FvH4_5g07750 FvH4_5g07750
malus_domestica	MD06G1147300.v1.1 MD06G1147500.v1.1 MD13G1113700.v1.1 MD14G1161200.v1.1 MD14G1161300.v1.1 MD16G1112900.v1.1 MD16G1113000.v1.1
prunus_persica	Prupe.1G232500_v2.0.a1 Prupe.5G154000_v2.0.a1 Prupe.5G154100_v2.0.a1
pyrus_communis	pycom06g13680 pycom06g13690 pycom13g09900 pycom14g13410 pycom16g09570 pycom16g09580
rosa_chinensis	RchiOBHm_Chr4g0430951 RchiOBHm_Chr7g0190671 RchiOBHm_Chr7g0190681
rosa_laevigata	RLG00000004504 RLG00000006935
rosa_multiflora	Rmu_co8020444.1_g000001 Rmu_sc0006263.1_g000015 Rmu_sc0006709.1_g000003 Rmu_sc0013571.1_g000005
rosa_roxburghii	Rroxscaffold_3G00264410 Rroxscaffold_5G00372640
rosa_rugosa	Rorug04G0247400 Rorug06G0506300 Rorug06G0506400
rosa_samantha	Rh4AG303200 Rh4AG304100 Rh4BG311400 Rh4CG327700 Rh4DG307500 Rh7AG112900 Rh7BG115400 Rh7CG117900 Rh7DG116800
rosa_wichuraiana	Rw4G026360 Rw7G009850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1439
AccB1I	GGYRCC	1 cut(s)	724
AccB7I	CCANNNNNTGG	1 cut(s)	41
AccBSI	CCGCTC	2 cut(s)	458, 838
AccI	GTMKAC	1 cut(s)	1323
AccII	CGCG	4 cut(s)	680, 752, 1067, 1560
AclWI	GGATC	2 cut(s)	26, 822
AcoI	YGGCCR	4 cut(s)	199, 283, 507, 666
AcsI	RAATTY	3 cut(s)	399, 465, 1185
AcvI	CACGTG	1 cut(s)	739
AcyI	GRCGYC	1 cut(s)	189
AdeI	CACNNNGTG	1 cut(s)	709
AfaI	GTAC	7 cut(s)	721, 743, 755, 1014, 1349, 1433, 1458
AfiI	CCNNNNNNNGG	7 cut(s)	41, 505, 618, 837, 874, 1333, 1441
AflIII	ACRYGT	3 cut(s)	533, 738, 1558
AgeI	ACCGGT	1 cut(s)	690
AgsI	TTSAA	2 cut(s)	260, 566
AjnI	CCWGG	1 cut(s)	1359
AleI	CACNNNNGTG	1 cut(s)	790
AluBI	AGCT	6 cut(s)	268, 387, 933, 991, 1100, 1421
AluI	AGCT	6 cut(s)	268, 387, 933, 991, 1100, 1421
Alw21I	GWGCWC	1 cut(s)	935
Alw26I	GTCTC	9 cut(s)	73, 370, 719, 894, 1024, 1037, 1136, 1142, 1275
AlwI	GGATC	2 cut(s)	26, 822
Ama87I	CYCGRG	4 cut(s)	218, 485, 1053, 1086
ApaI	GGGCCC	1 cut(s)	1076
ApeKI	GCWGC	4 cut(s)	951, 1003, 1244, 1418
ApoI	RAATTY	3 cut(s)	399, 465, 1185
AseI	ATTAAT	1 cut(s)	1619
AsiGI	ACCGGT	1 cut(s)	690
Asp700I	GAANNNNTTC	2 cut(s)	555, 1308
AspLEI	GCGC	4 cut(s)	962, 1067, 1069, 1540
AspS9I	GGNCC	6 cut(s)	489, 609, 1051, 1072, 1073, 1199
AsuC2I	CCSGG	4 cut(s)	607, 616, 1076, 1370
AsuHPI	GGTGA	6 cut(s)	115, 635, 784, 829, 901, 1238
AsuII	TTCGAA	1 cut(s)	685
AvaI	CYCGRG	4 cut(s)	218, 485, 1053, 1086
AvaII	GGWCC	3 cut(s)	489, 609, 1199
BaeGI	GKGCMC	3 cut(s)	868, 1076, 1162
BanI	GGYRCC	1 cut(s)	724
BanII	GRGCYC	2 cut(s)	935, 1076
BbrPI	CACGTG	1 cut(s)	739
Bbv12I	GWGCWC	1 cut(s)	935
BbvCI	CCTCAGC	1 cut(s)	992
BbvI	GCAGC	4 cut(s)	938, 990, 1231, 1430
BccI	CCATC	8 cut(s)	335, 500, 658, 790, 839, 907, 1260, 1407
BceAI	ACGGC	4 cut(s)	298, 416, 934, 1065
BciT130I	CCWGG	1 cut(s)	1361
BclI	TGATCA	1 cut(s)	1341
BcnI	CCSGG	4 cut(s)	607, 616, 1076, 1370
BcoDI	GTCTC	9 cut(s)	73, 370, 719, 894, 1024, 1037, 1136, 1142, 1275
BfoI	RGCGCY	1 cut(s)	963
BfuAI	ACCTGC	1 cut(s)	1439
BglI	GCCNNNNNGGC	2 cut(s)	857, 872
BisI	GCNGC	9 cut(s)	192, 249, 952, 955, 958, 1001, 1004, 1245, 1419
BlsI	GCNGC	9 cut(s)	193, 250, 953, 956, 959, 1002, 1005, 1246, 1420
Bme1390I	CCNGG	5 cut(s)	607, 616, 1076, 1361, 1370
Bme18I	GGWCC	3 cut(s)	489, 609, 1199
BmeT110I	CYCGRG	4 cut(s)	218, 485, 1053, 1086
BmgT120I	GGNCC	6 cut(s)	489, 609, 1051, 1072, 1073, 1199
BmiI	GGNNCC	7 cut(s)	490, 610, 699, 726, 842, 1074, 1481
BmrFI	CCNGG	5 cut(s)	607, 616, 1076, 1361, 1370
BmsI	GCATC	3 cut(s)	681, 1164, 1405
BpmI	CTGGAG	2 cut(s)	373, 1299
Bpu10I	CCTNAGC	1 cut(s)	992
Bpu14I	TTCGAA	1 cut(s)	685
BpuMI	CCSGG	4 cut(s)	607, 616, 1076, 1370
Bsa29I	ATCGAT	1 cut(s)	659
BsaAI	YACGTR	1 cut(s)	739
BsaHI	GRCGYC	1 cut(s)	189
BsaI	GGTCTC	1 cut(s)	73
BsaJI	CCNNGG	8 cut(s)	280, 294, 352, 484, 885, 1368, 1461, 1483
BsaWI	WCCGGW	2 cut(s)	645, 690
Bsc4I	CCNNNNNNNGG	7 cut(s)	41, 505, 618, 837, 874, 1333, 1441
Bse118I	RCCGGY	4 cut(s)	197, 668, 690, 871
Bse1I	ACTGG	6 cut(s)	390, 656, 792, 1156, 1228, 1282
Bse3DI	GCAATG	1 cut(s)	1521
BseBI	CCWGG	1 cut(s)	1361
BseCI	ATCGAT	1 cut(s)	659
BseDI	CCNNGG	8 cut(s)	280, 294, 352, 484, 885, 1368, 1461, 1483
BseGI	GGATG	8 cut(s)	327, 492, 602, 672, 801, 1041, 1271, 1399
BseLI	CCNNNNNNNGG	7 cut(s)	41, 505, 618, 837, 874, 1333, 1441
BseMI	GCAATG	1 cut(s)	1521
BseMII	CTCAG	5 cut(s)	68, 752, 983, 1137, 1272
BseNI	ACTGG	6 cut(s)	390, 656, 792, 1156, 1228, 1282
BsePI	GCGCGC	1 cut(s)	1065
BseRI	GAGGAG	3 cut(s)	173, 335, 1492
BseSI	GKGCMC	3 cut(s)	868, 1076, 1162
BseX3I	CGGCCG	1 cut(s)	507
BseXI	GCAGC	4 cut(s)	938, 990, 1231, 1430
BseYI	CCCAGC	2 cut(s)	264, 1069
BsgI	GTGCAG	1 cut(s)	1613
Bsh1236I	CGCG	4 cut(s)	680, 752, 1067, 1560
Bsh1285I	CGRYCG	5 cut(s)	510, 549, 695, 879, 1327
BshNI	GGYRCC	1 cut(s)	724
BshTI	ACCGGT	1 cut(s)	690
BshVI	ATCGAT	1 cut(s)	659
BsiEI	CGRYCG	5 cut(s)	510, 549, 695, 879, 1327
BsiHKAI	GWGCWC	1 cut(s)	935
BsiHKCI	CYCGRG	4 cut(s)	218, 485, 1053, 1086
BsiSI	CCGG	9 cut(s)	198, 606, 615, 646, 669, 691, 872, 1076, 1370
BslFI	GGGAC	4 cut(s)	378, 502, 622, 794
BslI	CCNNNNNNNGG	7 cut(s)	41, 505, 618, 837, 874, 1333, 1441
BsmAI	GTCTC	9 cut(s)	73, 370, 719, 894, 1024, 1037, 1136, 1142, 1275
BsmBI	CGTCTC	2 cut(s)	370, 1037
BsmFI	GGGAC	4 cut(s)	378, 502, 622, 794
BsmI	GAATGC	2 cut(s)	437, 1565
Bso31I	GGTCTC	1 cut(s)	73
BsoBI	CYCGRG	4 cut(s)	218, 485, 1053, 1086
Bsp119I	TTCGAA	1 cut(s)	685
Bsp120I	GGGCCC	1 cut(s)	1072
Bsp1286I	GDGCHC	4 cut(s)	868, 935, 1076, 1162
Bsp143I	GATC	5 cut(s)	31, 827, 945, 1341, 1509
Bsp19I	CCATGG	1 cut(s)	1461
BspCNI	CTCAG	5 cut(s)	69, 753, 984, 1138, 1271
BspDI	ATCGAT	1 cut(s)	659
BspFNI	CGCG	4 cut(s)	680, 752, 1067, 1560
BspLI	GGNNCC	7 cut(s)	490, 610, 699, 726, 842, 1074, 1481
BspMI	ACCTGC	1 cut(s)	1439
BspPI	GGATC	2 cut(s)	26, 822
BspT104I	TTCGAA	1 cut(s)	685
BspT107I	GGYRCC	1 cut(s)	724
BspTNI	GGTCTC	1 cut(s)	73
BsrBI	CCGCTC	2 cut(s)	458, 838
BsrDI	GCAATG	1 cut(s)	1521
BsrFI	RCCGGY	4 cut(s)	197, 668, 690, 871
BsrI	ACTGG	6 cut(s)	390, 656, 792, 1156, 1228, 1282
BssAI	RCCGGY	4 cut(s)	197, 668, 690, 871
BssECI	CCNNGG	8 cut(s)	280, 294, 352, 484, 885, 1368, 1461, 1483
BssHII	GCGCGC	1 cut(s)	1065
BssMI	GATC	5 cut(s)	31, 827, 945, 1341, 1509
BssNI	GRCGYC	1 cut(s)	189
BssT1I	CCWWGG	4 cut(s)	294, 885, 1461, 1483
Bst2UI	CCWGG	1 cut(s)	1361
Bst4CI	ACNGT	2 cut(s)	206, 724
Bst6I	CTCTTC	1 cut(s)	968
BstACI	GRCGYC	1 cut(s)	189
BstAPI	GCANNNNNTGC	1 cut(s)	1244
BstBAI	YACGTR	1 cut(s)	739
BstBI	TTCGAA	1 cut(s)	685
BstC8I	GCNNGC	6 cut(s)	385, 425, 429, 670, 873, 1067
BstDEI	CTNAG	5 cut(s)	77, 761, 992, 1146, 1258
BstDSI	CCRYGG	2 cut(s)	280, 1461
BstF5I	GGATG	8 cut(s)	327, 492, 602, 672, 801, 1041, 1271, 1399
BstFNI	CGCG	4 cut(s)	680, 752, 1067, 1560
BstH2I	RGCGCY	1 cut(s)	963
BstHHI	GCGC	4 cut(s)	962, 1067, 1069, 1540
BstKTI	GATC	5 cut(s)	34, 830, 948, 1344, 1512
BstMAI	GTCTC	9 cut(s)	73, 370, 719, 894, 1024, 1037, 1136, 1142, 1275
BstMBI	GATC	5 cut(s)	31, 827, 945, 1341, 1509
BstMCI	CGRYCG	5 cut(s)	510, 549, 695, 879, 1327
BstNI	CCWGG	1 cut(s)	1361
BstNSI	RCATGY	1 cut(s)	537
BstSCI	CCNGG	5 cut(s)	605, 614, 1074, 1359, 1368
BstSLI	GKGCMC	3 cut(s)	868, 1076, 1162
BstUI	CGCG	4 cut(s)	680, 752, 1067, 1560
BstV1I	GCAGC	4 cut(s)	938, 990, 1231, 1430
BstX2I	RGATCY	1 cut(s)	827
BstXI	CCANNNNNNTGG	1 cut(s)	1138
BstYI	RGATCY	1 cut(s)	827
BstZI	CGGCCG	1 cut(s)	507
Bsu15I	ATCGAT	1 cut(s)	659
BsuTUI	ATCGAT	1 cut(s)	659
BtgI	CCRYGG	2 cut(s)	280, 1461
BtsCI	GGATG	8 cut(s)	327, 492, 602, 672, 801, 1041, 1271, 1399
BtsI	GCAGTG	1 cut(s)	1162
BtsIMutI	CAGTG	4 cut(s)	785, 1162, 1221, 1579
BveI	ACCTGC	1 cut(s)	1439
Cac8I	GCNNGC	6 cut(s)	385, 425, 429, 670, 873, 1067
CfoI	GCGC	4 cut(s)	962, 1067, 1069, 1540
Cfr10I	RCCGGY	4 cut(s)	197, 668, 690, 871
Cfr13I	GGNCC	6 cut(s)	489, 609, 1051, 1072, 1073, 1199
ClaI	ATCGAT	1 cut(s)	659
CseI	GACGC	2 cut(s)	197, 1566
CsiI	ACCWGGT	1 cut(s)	1359
Csp6I	GTAC	7 cut(s)	720, 742, 754, 1013, 1348, 1432, 1457
CspAI	ACCGGT	1 cut(s)	690
CviAII	CATG	5 cut(s)	523, 534, 856, 1178, 1462
CviQI	GTAC	7 cut(s)	720, 742, 754, 1013, 1348, 1432, 1457
DdeI	CTNAG	5 cut(s)	77, 761, 992, 1146, 1258
DpnI	GATC	5 cut(s)	33, 829, 947, 1343, 1511
DpnII	GATC	5 cut(s)	31, 827, 945, 1341, 1509
DraI	TTTAAA	1 cut(s)	813
DraIII	CACNNNGTG	1 cut(s)	709
EaeI	YGGCCR	4 cut(s)	199, 283, 507, 666
EagI	CGGCCG	1 cut(s)	507
Eam1104I	CTCTTC	1 cut(s)	968
EarI	CTCTTC	1 cut(s)	968
EciI	GGCGGA	3 cut(s)	439, 470, 1388
Ecl136II	GAGCTC	1 cut(s)	933
EclXI	CGGCCG	1 cut(s)	507
Eco130I	CCWWGG	4 cut(s)	294, 885, 1461, 1483
Eco24I	GRGCYC	2 cut(s)	935, 1076
Eco31I	GGTCTC	1 cut(s)	73
Eco32I	GATATC	1 cut(s)	657
Eco47I	GGWCC	3 cut(s)	489, 609, 1199
Eco52I	CGGCCG	1 cut(s)	507
Eco53kI	GAGCTC	1 cut(s)	933
Eco72I	CACGTG	1 cut(s)	739
Eco88I	CYCGRG	4 cut(s)	218, 485, 1053, 1086
EcoICRI	GAGCTC	1 cut(s)	933
EcoRII	CCWGG	1 cut(s)	1359
EcoRV	GATATC	1 cut(s)	657
EcoT14I	CCWWGG	4 cut(s)	294, 885, 1461, 1483
EcoT22I	ATGCAT	1 cut(s)	1179
EcoT38I	GRGCYC	2 cut(s)	935, 1076
ErhI	CCWWGG	4 cut(s)	294, 885, 1461, 1483
Esp3I	CGTCTC	2 cut(s)	370, 1037
FaeI	CATG	5 cut(s)	526, 537, 859, 1181, 1465
FaqI	GGGAC	4 cut(s)	378, 502, 622, 794
FatI	CATG	5 cut(s)	522, 533, 855, 1177, 1461
FauI	CCCGC	5 cut(s)	184, 388, 413, 436, 876
FauNDI	CATATG	1 cut(s)	419
FbaI	TGATCA	1 cut(s)	1341
FblI	GTMKAC	1 cut(s)	1323
Fnu4HI	GCNGC	9 cut(s)	192, 249, 952, 955, 958, 1001, 1004, 1245, 1419
FokI	GGATG	8 cut(s)	314, 479, 589, 659, 808, 1048, 1278, 1386
FriOI	GRGCYC	2 cut(s)	935, 1076
Fsp4HI	GCNGC	9 cut(s)	192, 249, 952, 955, 958, 1001, 1004, 1245, 1419
GlaI	GCGC	4 cut(s)	961, 1066, 1068, 1539
GluI	GCNGC	9 cut(s)	192, 249, 952, 955, 958, 1001, 1004, 1245, 1419
GsaI	CCCAGC	2 cut(s)	268, 1073
GsuI	CTGGAG	2 cut(s)	373, 1299
HaeII	RGCGCY	1 cut(s)	963
HapII	CCGG	9 cut(s)	198, 606, 615, 646, 669, 691, 872, 1076, 1370
HgaI	GACGC	2 cut(s)	197, 1566
HhaI	GCGC	4 cut(s)	962, 1067, 1069, 1540
Hin1I	GRCGYC	1 cut(s)	189
Hin1II	CATG	5 cut(s)	526, 537, 859, 1181, 1465
Hin6I	GCGC	4 cut(s)	960, 1065, 1067, 1538
HinP1I	GCGC	4 cut(s)	960, 1065, 1067, 1538
HincII	GTYRAC	2 cut(s)	639, 1324
HindII	GTYRAC	2 cut(s)	639, 1324
HpaII	CCGG	9 cut(s)	198, 606, 615, 646, 669, 691, 872, 1076, 1370
HphI	GGTGA	6 cut(s)	115, 635, 784, 829, 901, 1238
Hpy166II	GTNNAC	3 cut(s)	639, 742, 1324
Hpy188I	TCNGA	6 cut(s)	239, 540, 580, 845, 1120, 1261
Hpy188III	TCNNGA	5 cut(s)	395, 487, 985, 1403, 1474
Hpy8I	GTNNAC	3 cut(s)	639, 742, 1324
Hpy99I	CGWCG	6 cut(s)	191, 437, 1051, 1325, 1475, 1560
HpyCH4III	ACNGT	2 cut(s)	206, 724
HpyCH4IV	ACGT	3 cut(s)	738, 1011, 1206
HpyCH4V	TGCA	9 cut(s)	423, 940, 1177, 1247, 1418, 1526, 1577, 1594, 1631
HpyF3I	CTNAG	5 cut(s)	77, 761, 992, 1146, 1258
HpySE526I	ACGT	3 cut(s)	738, 1011, 1206
Hsp92I	GRCGYC	1 cut(s)	189
Hsp92II	CATG	5 cut(s)	526, 537, 859, 1181, 1465
HspAI	GCGC	4 cut(s)	960, 1065, 1067, 1538
KroI	GCCGGC	2 cut(s)	668, 871
KroNI	GCCGGC	2 cut(s)	670, 873
Ksp22I	TGATCA	1 cut(s)	1341
Kzo9I	GATC	5 cut(s)	31, 827, 945, 1341, 1509
LmnI	GCTCC	6 cut(s)	392, 703, 938, 967, 1097, 1479
Lsp1109I	GCAGC	4 cut(s)	938, 990, 1231, 1430
LweI	GCATC	3 cut(s)	681, 1164, 1405
MabI	ACCWGGT	1 cut(s)	1359
MaeII	ACGT	3 cut(s)	738, 1011, 1206
MaeIII	GTNAC	3 cut(s)	772, 1217, 1226
MalI	GATC	5 cut(s)	33, 829, 947, 1343, 1511
MbiI	CCGCTC	2 cut(s)	458, 838
MboI	GATC	5 cut(s)	31, 827, 945, 1341, 1509
MboII	GAAGA	4 cut(s)	985, 1091, 1244, 1561
MflI	RGATCY	1 cut(s)	827
MhlI	GDGCHC	4 cut(s)	868, 935, 1076, 1162
MluCI	AATT	6 cut(s)	399, 465, 1185, 1248, 1337, 1616
MluI	ACGCGT	1 cut(s)	1558
MlyI	GAGTC	5 cut(s)	243, 497, 527, 634, 1250
MmeI	TCCRAC	2 cut(s)	399, 1359
Mph1103I	ATGCAT	1 cut(s)	1179
MreI	CGCCGGCG	1 cut(s)	871
MroNI	GCCGGC	2 cut(s)	668, 871
MroXI	GAANNNNTTC	2 cut(s)	555, 1308
MseI	TTAA	3 cut(s)	812, 1271, 1619
MslI	CAYNNNNRTG	2 cut(s)	527, 790
MspA1I	CMGCKG	2 cut(s)	967, 1421
MspI	CCGG	9 cut(s)	198, 606, 615, 646, 669, 691, 872, 1076, 1370
MspR9I	CCNGG	5 cut(s)	607, 616, 1076, 1361, 1370
Mva1269I	GAATGC	2 cut(s)	437, 1565
MvaI	CCWGG	1 cut(s)	1361
MvnI	CGCG	4 cut(s)	680, 752, 1067, 1560
NaeI	GCCGGC	2 cut(s)	670, 873
NciI	CCSGG	4 cut(s)	607, 616, 1076, 1370
NcoI	CCATGG	1 cut(s)	1461
NdeI	CATATG	1 cut(s)	419
NdeII	GATC	5 cut(s)	31, 827, 945, 1341, 1509
NgoMIV	GCCGGC	2 cut(s)	668, 871
NlaIII	CATG	5 cut(s)	526, 537, 859, 1181, 1465
NlaIV	GGNNCC	7 cut(s)	490, 610, 699, 726, 842, 1074, 1481
NmeAIII	GCCGAG	3 cut(s)	249, 485, 535
NmuCI	GTSAC	2 cut(s)	772, 1226
NsiI	ATGCAT	1 cut(s)	1179
NspI	RCATGY	1 cut(s)	537
NspV	TTCGAA	1 cut(s)	685
OliI	CACNNNNGTG	1 cut(s)	790
PauI	GCGCGC	1 cut(s)	1065
PciI	ACATGT	1 cut(s)	533
PcsI	WCGNNNNNNNCGW	1 cut(s)	360
PctI	GAATGC	2 cut(s)	437, 1565
PdiI	GCCGGC	2 cut(s)	670, 873
PdmI	GAANNNNTTC	2 cut(s)	555, 1308
PfeI	GAWTC	7 cut(s)	551, 592, 846, 1329, 1426, 1502, 1552
PflFI	GACNNNGTC	1 cut(s)	1409
PflMI	CCANNNNNTGG	1 cut(s)	41
PfoI	TCCNGGA	1 cut(s)	605
PinAI	ACCGGT	1 cut(s)	690
PkrI	GCNGC	9 cut(s)	193, 250, 953, 956, 959, 1002, 1005, 1246, 1420
PleI	GAGTC	5 cut(s)	242, 497, 526, 634, 1250
PmaCI	CACGTG	1 cut(s)	739
PmlI	CACGTG	1 cut(s)	739
PpsI	GAGTC	5 cut(s)	242, 497, 526, 634, 1250
Ppu21I	YACGTR	1 cut(s)	739
PscI	ACATGT	1 cut(s)	533
PshBI	ATTAAT	1 cut(s)	1619
Psp124BI	GAGCTC	1 cut(s)	935
Psp6I	CCWGG	1 cut(s)	1359
PspCI	CACGTG	1 cut(s)	739
PspFI	CCCAGC	2 cut(s)	264, 1069
PspGI	CCWGG	1 cut(s)	1359
PspN4I	GGNNCC	7 cut(s)	490, 610, 699, 726, 842, 1074, 1481
PspOMI	GGGCCC	1 cut(s)	1072
PspPI	GGNCC	6 cut(s)	489, 609, 1051, 1072, 1073, 1199
PsuI	RGATCY	1 cut(s)	827
PsyI	GACNNNGTC	1 cut(s)	1409
PteI	GCGCGC	1 cut(s)	1065
PvuII	CAGCTG	1 cut(s)	1421
RsaI	GTAC	7 cut(s)	721, 743, 755, 1014, 1349, 1433, 1458
RsaNI	GTAC	7 cut(s)	720, 742, 754, 1013, 1348, 1432, 1457
RseI	CAYNNNNRTG	2 cut(s)	527, 790
SacI	GAGCTC	1 cut(s)	935
SalI	GTCGAC	1 cut(s)	1322
SaqAI	TTAA	3 cut(s)	812, 1271, 1619
SatI	GCNGC	9 cut(s)	192, 249, 952, 955, 958, 1001, 1004, 1245, 1419
Sau3AI	GATC	5 cut(s)	31, 827, 945, 1341, 1509
Sau96I	GGNCC	6 cut(s)	489, 609, 1051, 1072, 1073, 1199
SchI	GAGTC	5 cut(s)	243, 497, 527, 634, 1250
ScrFI	CCNGG	5 cut(s)	607, 616, 1076, 1361, 1370
SduI	GDGCHC	4 cut(s)	868, 935, 1076, 1162
SexAI	ACCWGGT	1 cut(s)	1359
SfaNI	GCATC	3 cut(s)	681, 1164, 1405
SfuI	TTCGAA	1 cut(s)	685
SgrAI	CRCCGGYG	1 cut(s)	871
SinI	GGWCC	3 cut(s)	489, 609, 1199
SmiMI	CAYNNNNRTG	2 cut(s)	527, 790
Sse9I	AATT	6 cut(s)	399, 465, 1185, 1248, 1337, 1616
SstI	GAGCTC	1 cut(s)	935
StyD4I	CCNGG	5 cut(s)	605, 614, 1074, 1359, 1368
StyI	CCWWGG	4 cut(s)	294, 885, 1461, 1483
TaaI	ACNGT	2 cut(s)	206, 724
TaiI	ACGT	3 cut(s)	741, 1014, 1209
TaqII	GACCGA	2 cut(s)	563, 1341
TasI	AATT	6 cut(s)	399, 465, 1185, 1248, 1337, 1616
TatI	WGTACW	1 cut(s)	1347
TauI	GCSGC	5 cut(s)	194, 251, 957, 960, 1003
TfiI	GAWTC	7 cut(s)	551, 592, 846, 1329, 1426, 1502, 1552
Tru1I	TTAA	3 cut(s)	812, 1271, 1619
Tru9I	TTAA	3 cut(s)	812, 1271, 1619
TscAI	CASTG	4 cut(s)	792, 1162, 1228, 1579
TseFI	GTSAC	2 cut(s)	772, 1226
TseI	GCWGC	4 cut(s)	951, 1003, 1244, 1418
Tsp45I	GTSAC	2 cut(s)	772, 1226
TspDTI	ATGAA	7 cut(s)	214, 1226, 1317, 1382, 1510, 1515, 1558
TspGWI	ACGGA	4 cut(s)	912, 1055, 1419, 1529
TspRI	CASTG	4 cut(s)	792, 1162, 1228, 1579
Tth111I	GACNNNGTC	1 cut(s)	1409
Van91I	CCANNNNNTGG	1 cut(s)	41
VpaK11BI	GGWCC	3 cut(s)	489, 609, 1199
VspI	ATTAAT	1 cut(s)	1619
XapI	RAATTY	3 cut(s)	399, 465, 1185
XceI	RCATGY	1 cut(s)	537
XmiI	GTMKAC	1 cut(s)	1323
XmnI	GAANNNNTTC	2 cut(s)	555, 1308
Zsp2I	ATGCAT	1 cut(s)	1179

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.