Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001900

Physical Location & Seq

Genomic Coordinates Forward (+)

7910314 .. 7912825

Gene Structure 2512 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1113000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1647 bp

ATGGCGACTCAACAGAAGAGCAGCAGCATTGATCCAAAGAGTGGATTTTGCTCAAAGACCAAGACTTTCCACAGCCTGAGACCCAGATCCCAACCCCCTCCCCAAACCACGCCTCTTTCCCTCACCGACTATCTCCTCTCCCGCCTCCACCACTCCCCTCCCCTGCCTTCCACTCCCGCCCTCCTCGACGCCGCCACCGGCCACCATATTCTCTACCCCGAGTTCATTTTTCGAGTCCGAACCCTTGCCGCCTCGCTTCAATGCCAGCTCGGCCTCTCCCACGGCCAATGCGCCTTCGTTCTCTCCCCCAACTCCCTCCACCTCCCCATCCTCCACCTCTCTCTCCTCTCCCTCGGCGTTATCGTTTCCCCTTCCAACCCCGCCAGCTCCAATCCCGAAGTTTCCCACCAAATCAGCATATGCAGACCCACCGTCGCATTCGCCACCACTGCCACCGCTCACAAAATCCCCAACTCCCTCCGCCTCGGGACCATCCTCCTCGACTCGGCCGAGTTCGAGTCCATGATGATGACCTATCCGAGTTCGGCCGAATCGCTTCGAACTGAAGTGAGCCAATCAGACACCGCCACGATTCTCTACTCATCGGGGACCACCGGGAGGGTCAAAGGCGTGGAGTTTACTCACCGGAACTGGATATGGATGATGGCGGGCATCCGCGCGGTTCGAAACGCGTCCGCTCCACACGCTGTATGTCTCTGTACCGTGCCGTTTTTCCACTCGTACGGGTTCGCGTACTGCTTCATAGTTCTGTTGACGGGGGACACGCTAGCGTTCACGGGAAGGTTCGATTTGAAGGTGATGATGAGATCCATCGAGCGGTTCCGAATCACCGAGGTGGCCTGCGCCCCGCCTGTGGTCGTGGCCTTGGTGAAGCACGGGGACATAAACGTGATGGACGGTTACGATTTGAGCTCCCTACAAGTGATCGGCTGCGGCGCCGCTCCGCTGGCGAAGAGCGTGATCGAGAAGCTGAGGAAGCGGCTGCCCCACGTACAAATCTCACAGGGATATGGGATGACGGAGATGACGGGCCGAGTATGTGGCCCGGTGGGCCCGGAAGAAACCCGAGTGGAAGGAGCTAATGGAAGGCTTACGTCAGATATTGAGGTCAAGATTGTGGAGCCTGAGACTGGCACTGCCCTGCTTCCTTTGATGCATGGGGAAATTTGGGTTAGAGGACCATACGTTATGAAAGGTTACGTTGGTGATGAAGATGCAACTGCCACAACTTTGGACTCGGAAGGATGGTTAAAAACTGGAGACATTTGCTACATAGACAATGAAGGTTTTCTATATTTCGTCGACCGAATCAAGGAATTGATCAAGTACAAAGGCTACCAGGTTGCCCCGGTGGAGTTGGAGGATCTGCTTCACTCCCATCCAGACATTGTTGATGCAGCTGTGATTCCGTACCCAGATGAGGAAGCAGGTCAAGTACCCATGGCCTTCGTCGTGAGGAGCCATGGAAGCGCCATTGATGAATCACAGATCAAGGATTTTATTGCAAAACAGGTTGCGCCATATAAGAAGATACGACGCGTAGCATTCATCAATGCAATACCAAAGAATGTGCAGGGAAAAGTGTTGAGGAAGGAACTAATCAAACTTGCATTATCCAAGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004321 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0008150 GO:0008152 GO:0009058 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0016053 GO:0016408 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0032787 GO:0042445 GO:0042446 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

549

Amino Acids

60.02

Weight (kDa)

7.96

Isoelectric Point (pI)

40.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	56 - 407	3.8e-74	AMP-binding enzyme
AMP-binding_C	PF13193	459 - 534	3.6e-17	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001900)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63380
fragaria_vesca	FvH4_4g24170 FvH4_5g07750 FvH4_5g07750 FvH4_5g07750
malus_domestica	MD06G1147300.v1.1 MD06G1147500.v1.1 MD13G1113700.v1.1 MD14G1161200.v1.1 MD14G1161300.v1.1 MD16G1112900.v1.1 MD16G1113000.v1.1
prunus_persica	Prupe.1G232500_v2.0.a1 Prupe.5G154000_v2.0.a1 Prupe.5G154100_v2.0.a1
pyrus_communis	pycom06g13680 pycom06g13690 pycom13g09900 pycom14g13410 pycom16g09570 pycom16g09580
rosa_chinensis	RchiOBHm_Chr4g0430951 RchiOBHm_Chr7g0190671 RchiOBHm_Chr7g0190681
rosa_laevigata	RLG00000004504 RLG00000006935
rosa_multiflora	Rmu_co8020444.1_g000001 Rmu_sc0006263.1_g000015 Rmu_sc0006709.1_g000003 Rmu_sc0013571.1_g000005
rosa_roxburghii	Rroxscaffold_3G00264410 Rroxscaffold_5G00372640
rosa_rugosa	Rorug04G0247400 Rorug06G0506300 Rorug06G0506400
rosa_samantha	Rh4AG303200 Rh4AG304100 Rh4BG311400 Rh4CG327700 Rh4DG307500 Rh7AG112900 Rh7BG115400 Rh7CG117900 Rh7DG116800
rosa_wichuraiana	Rw4G026360 Rw7G009850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1439
AccB1I	GGYRCC	1 cut(s)	956
AccB7I	CCANNNNNTGG	1 cut(s)	41
AccBSI	CCGCTC	4 cut(s)	458, 698, 838, 962
AccI	GTMKAC	1 cut(s)	1323
AccII	CGCG	5 cut(s)	678, 680, 692, 752, 1560
AclWI	GGATC	4 cut(s)	26, 81, 822, 1392
AcoI	YGGCCR	4 cut(s)	199, 283, 507, 546
AcsI	RAATTY	1 cut(s)	1185
AcuI	CTGAAG	1 cut(s)	585
AcyI	GRCGYC	2 cut(s)	189, 957
AfaI	GTAC	7 cut(s)	721, 743, 755, 1014, 1349, 1433, 1458
AfiI	CCNNNNNNNGG	8 cut(s)	41, 505, 618, 837, 874, 1151, 1333, 1441
AflIII	ACRYGT	2 cut(s)	690, 1558
AgsI	TTSAA	2 cut(s)	260, 814
AhdI	GACNNNNNGTC	1 cut(s)	431
AjnI	CCWGG	1 cut(s)	1359
AleI	CACNNNNGTG	1 cut(s)	854
AluBI	AGCT	6 cut(s)	268, 387, 933, 991, 1100, 1421
AluI	AGCT	6 cut(s)	268, 387, 933, 991, 1100, 1421
Alw21I	GWGCWC	1 cut(s)	935
Alw26I	GTCTC	4 cut(s)	73, 719, 1142, 1275
AlwI	GGATC	4 cut(s)	26, 81, 822, 1392
Ama87I	CYCGRG	3 cut(s)	218, 485, 1086
ApaI	GGGCCC	1 cut(s)	1076
ApeKI	GCWGC	5 cut(s)	21, 24, 951, 1003, 1418
ApoI	RAATTY	1 cut(s)	1185
Asp700I	GAANNNNTTC	2 cut(s)	555, 1308
AspLEI	GCGC	6 cut(s)	293, 680, 866, 959, 1493, 1540
AspS9I	GGNCC	7 cut(s)	489, 609, 1051, 1064, 1072, 1073, 1199
AsuC2I	CCSGG	4 cut(s)	616, 1067, 1076, 1370
AsuHPI	GGTGA	6 cut(s)	115, 635, 829, 841, 901, 1238
AsuII	TTCGAA	2 cut(s)	559, 685
AsuNHI	GCTAGC	1 cut(s)	787
AvaI	CYCGRG	3 cut(s)	218, 485, 1086
AvaII	GGWCC	3 cut(s)	489, 609, 1199
BaeGI	GKGCMC	1 cut(s)	1076
BanI	GGYRCC	1 cut(s)	956
BanII	GRGCYC	2 cut(s)	935, 1076
Bbv12I	GWGCWC	1 cut(s)	935
BbvCI	CCTCAGC	1 cut(s)	992
BbvI	GCAGC	5 cut(s)	33, 36, 938, 990, 1430
BccI	CCATC	7 cut(s)	335, 500, 658, 839, 907, 1260, 1407
BceAI	ACGGC	2 cut(s)	298, 712
BciT130I	CCWGG	1 cut(s)	1361
BclI	TGATCA	1 cut(s)	1341
BcnI	CCSGG	4 cut(s)	616, 1067, 1076, 1370
BcoDI	GTCTC	4 cut(s)	73, 719, 1142, 1275
BfaI	CTAG	1 cut(s)	788
BfoI	RGCGCY	2 cut(s)	960, 1494
BfuAI	ACCTGC	1 cut(s)	1439
BglI	GCCNNNNNGGC	2 cut(s)	270, 1071
Bme1390I	CCNGG	5 cut(s)	616, 1067, 1076, 1361, 1370
Bme18I	GGWCC	3 cut(s)	489, 609, 1199
BmeRI	GACNNNNNGTC	1 cut(s)	431
BmeT110I	CYCGRG	3 cut(s)	218, 485, 1086
BmgT120I	GGNCC	7 cut(s)	489, 609, 1051, 1064, 1072, 1073, 1199
BmiI	GGNNCC	7 cut(s)	490, 610, 842, 958, 1074, 1143, 1481
BmrFI	CCNGG	5 cut(s)	616, 1067, 1076, 1361, 1370
BmsI	GCATC	4 cut(s)	681, 1164, 1225, 1405
BmtI	GCTAGC	1 cut(s)	791
BpmI	CTGGAG	1 cut(s)	1299
Bpu10I	CCTNAGC	1 cut(s)	992
Bpu14I	TTCGAA	2 cut(s)	559, 685
BpuMI	CCSGG	4 cut(s)	616, 1067, 1076, 1370
BsaAI	YACGTR	1 cut(s)	1012
BsaHI	GRCGYC	2 cut(s)	189, 957
BsaI	GGTCTC	1 cut(s)	73
BsaJI	CCNNGG	8 cut(s)	280, 352, 484, 852, 885, 1368, 1461, 1483
BsaWI	WCCGGW	1 cut(s)	645
Bsc4I	CCNNNNNNNGG	8 cut(s)	41, 505, 618, 837, 874, 1151, 1333, 1441
Bse118I	RCCGGY	1 cut(s)	197
Bse1I	ACTGG	3 cut(s)	656, 1156, 1282
BseBI	CCWGG	1 cut(s)	1361
BseDI	CCNNGG	8 cut(s)	280, 352, 484, 852, 885, 1368, 1461, 1483
BseGI	GGATG	7 cut(s)	327, 492, 666, 672, 1041, 1271, 1399
BseLI	CCNNNNNNNGG	8 cut(s)	41, 505, 618, 837, 874, 1151, 1333, 1441
BseMII	CTCAG	3 cut(s)	68, 983, 1137
BseNI	ACTGG	3 cut(s)	656, 1156, 1282
BseRI	GAGGAG	5 cut(s)	125, 173, 335, 488, 1492
BseSI	GKGCMC	1 cut(s)	1076
BseX3I	CGGCCG	2 cut(s)	507, 546
BseXI	GCAGC	5 cut(s)	33, 36, 938, 990, 1430
BsgI	GTGCAG	1 cut(s)	1613
Bsh1236I	CGCG	5 cut(s)	678, 680, 692, 752, 1560
Bsh1285I	CGRYCG	3 cut(s)	510, 549, 1327
BshNI	GGYRCC	1 cut(s)	956
BsiEI	CGRYCG	3 cut(s)	510, 549, 1327
BsiHKAI	GWGCWC	1 cut(s)	935
BsiHKCI	CYCGRG	3 cut(s)	218, 485, 1086
BsiSI	CCGG	6 cut(s)	198, 615, 646, 1067, 1076, 1370
BsiWI	CGTACG	1 cut(s)	741
BslFI	GGGAC	4 cut(s)	502, 622, 794, 914
BslI	CCNNNNNNNGG	8 cut(s)	41, 505, 618, 837, 874, 1151, 1333, 1441
BsmAI	GTCTC	4 cut(s)	73, 719, 1142, 1275
BsmFI	GGGAC	4 cut(s)	502, 622, 794, 914
BsmI	GAATGC	2 cut(s)	437, 1565
Bso31I	GGTCTC	1 cut(s)	73
BsoBI	CYCGRG	3 cut(s)	218, 485, 1086
Bsp119I	TTCGAA	2 cut(s)	559, 685
Bsp120I	GGGCCC	1 cut(s)	1072
Bsp1286I	GDGCHC	2 cut(s)	935, 1076
Bsp143I	GATC	8 cut(s)	31, 86, 827, 945, 981, 1341, 1384, 1509
Bsp19I	CCATGG	2 cut(s)	1461, 1483
BspCNI	CTCAG	3 cut(s)	69, 984, 1138
BspFNI	CGCG	5 cut(s)	678, 680, 692, 752, 1560
BspLI	GGNNCC	7 cut(s)	490, 610, 842, 958, 1074, 1143, 1481
BspMI	ACCTGC	1 cut(s)	1439
BspOI	GCTAGC	1 cut(s)	791
BspPI	GGATC	4 cut(s)	26, 81, 822, 1392
BspQI	GCTCTTC	2 cut(s)	11, 968
BspT104I	TTCGAA	2 cut(s)	559, 685
BspT107I	GGYRCC	1 cut(s)	956
BspTNI	GGTCTC	1 cut(s)	73
BsrBI	CCGCTC	4 cut(s)	458, 698, 838, 962
BsrFI	RCCGGY	1 cut(s)	197
BsrI	ACTGG	3 cut(s)	656, 1156, 1282
BssAI	RCCGGY	1 cut(s)	197
BssECI	CCNNGG	8 cut(s)	280, 352, 484, 852, 885, 1368, 1461, 1483
BssMI	GATC	8 cut(s)	31, 86, 827, 945, 981, 1341, 1384, 1509
BssNI	GRCGYC	2 cut(s)	189, 957
BssT1I	CCWWGG	3 cut(s)	885, 1461, 1483
Bst2UI	CCWGG	1 cut(s)	1361
Bst4CI	ACNGT	3 cut(s)	433, 724, 920
Bst6I	CTCTTC	2 cut(s)	11, 968
BstACI	GRCGYC	2 cut(s)	189, 957
BstBAI	YACGTR	1 cut(s)	1012
BstBI	TTCGAA	2 cut(s)	559, 685
BstC8I	GCNNGC	6 cut(s)	266, 385, 670, 789, 862, 969
BstDEI	CTNAG	3 cut(s)	77, 992, 1146
BstDSI	CCRYGG	3 cut(s)	280, 1461, 1483
BstF5I	GGATG	7 cut(s)	327, 492, 666, 672, 1041, 1271, 1399
BstFNI	CGCG	5 cut(s)	678, 680, 692, 752, 1560
BstH2I	RGCGCY	2 cut(s)	960, 1494
BstHHI	GCGC	6 cut(s)	293, 680, 866, 959, 1493, 1540
BstKTI	GATC	8 cut(s)	34, 89, 830, 948, 984, 1344, 1387, 1512
BstMAI	GTCTC	4 cut(s)	73, 719, 1142, 1275
BstMBI	GATC	8 cut(s)	31, 86, 827, 945, 981, 1341, 1384, 1509
BstMCI	CGRYCG	3 cut(s)	510, 549, 1327
BstMWI	GCNNNNNNNGC	7 cut(s)	270, 449, 704, 968, 997, 1071, 1488
BstNI	CCWGG	1 cut(s)	1361
BstSCI	CCNGG	5 cut(s)	614, 1065, 1074, 1359, 1368
BstSLI	GKGCMC	1 cut(s)	1076
BstUI	CGCG	5 cut(s)	678, 680, 692, 752, 1560
BstV1I	GCAGC	5 cut(s)	33, 36, 938, 990, 1430
BstX2I	RGATCY	3 cut(s)	86, 827, 1384
BstXI	CCANNNNNNTGG	1 cut(s)	1252
BstYI	RGATCY	3 cut(s)	86, 827, 1384
BstZI	CGGCCG	2 cut(s)	507, 546
BtgI	CCRYGG	3 cut(s)	280, 1461, 1483
BtsCI	GGATG	7 cut(s)	327, 492, 666, 672, 1041, 1271, 1399
BtsI	GCAGTG	2 cut(s)	447, 1155
BtsIMutI	CAGTG	2 cut(s)	447, 1155
BveI	ACCTGC	1 cut(s)	1439
Cac8I	GCNNGC	6 cut(s)	266, 385, 670, 789, 862, 969
CfoI	GCGC	6 cut(s)	293, 680, 866, 959, 1493, 1540
Cfr10I	RCCGGY	1 cut(s)	197
Cfr13I	GGNCC	7 cut(s)	489, 609, 1051, 1064, 1072, 1073, 1199
CseI	GACGC	3 cut(s)	197, 681, 1566
CsiI	ACCWGGT	1 cut(s)	1359
Csp6I	GTAC	7 cut(s)	720, 742, 754, 1013, 1348, 1432, 1457
CviAII	CATG	4 cut(s)	523, 1178, 1462, 1484
CviQI	GTAC	7 cut(s)	720, 742, 754, 1013, 1348, 1432, 1457
DdeI	CTNAG	3 cut(s)	77, 992, 1146
DinI	GGCGCC	1 cut(s)	958
DpnI	GATC	8 cut(s)	33, 88, 829, 947, 983, 1343, 1386, 1511
DpnII	GATC	8 cut(s)	31, 86, 827, 945, 981, 1341, 1384, 1509
DriI	GACNNNNNGTC	1 cut(s)	431
EaeI	YGGCCR	4 cut(s)	199, 283, 507, 546
EagI	CGGCCG	2 cut(s)	507, 546
Eam1104I	CTCTTC	2 cut(s)	11, 968
Eam1105I	GACNNNNNGTC	1 cut(s)	431
EarI	CTCTTC	2 cut(s)	11, 968
EciI	GGCGGA	1 cut(s)	470
Ecl136II	GAGCTC	1 cut(s)	933
EclXI	CGGCCG	2 cut(s)	507, 546
Eco130I	CCWWGG	3 cut(s)	885, 1461, 1483
Eco24I	GRGCYC	2 cut(s)	935, 1076
Eco31I	GGTCTC	1 cut(s)	73
Eco47I	GGWCC	3 cut(s)	489, 609, 1199
Eco52I	CGGCCG	2 cut(s)	507, 546
Eco53kI	GAGCTC	1 cut(s)	933
Eco57I	CTGAAG	1 cut(s)	585
Eco88I	CYCGRG	3 cut(s)	218, 485, 1086
EcoICRI	GAGCTC	1 cut(s)	933
EcoRII	CCWGG	1 cut(s)	1359
EcoT14I	CCWWGG	3 cut(s)	885, 1461, 1483
EcoT22I	ATGCAT	1 cut(s)	1179
EcoT38I	GRGCYC	2 cut(s)	935, 1076
EgeI	GGCGCC	1 cut(s)	958
EheI	GGCGCC	1 cut(s)	958
ErhI	CCWWGG	3 cut(s)	885, 1461, 1483
FaeI	CATG	4 cut(s)	526, 1181, 1465, 1487
FaqI	GGGAC	4 cut(s)	502, 622, 794, 914
FatI	CATG	4 cut(s)	522, 1177, 1461, 1483
FauI	CCCGC	5 cut(s)	149, 184, 388, 661, 876
FauNDI	CATATG	1 cut(s)	419
FbaI	TGATCA	1 cut(s)	1341
FblI	GTMKAC	1 cut(s)	1323
FokI	GGATG	7 cut(s)	314, 479, 659, 673, 1048, 1278, 1386
FriOI	GRGCYC	2 cut(s)	935, 1076
FspBI	CTAG	1 cut(s)	788
GlaI	GCGC	6 cut(s)	292, 679, 865, 958, 1492, 1539
GsuI	CTGGAG	1 cut(s)	1299
HaeII	RGCGCY	2 cut(s)	960, 1494
HapII	CCGG	6 cut(s)	198, 615, 646, 1067, 1076, 1370
HgaI	GACGC	3 cut(s)	197, 681, 1566
HhaI	GCGC	6 cut(s)	293, 680, 866, 959, 1493, 1540
Hin1I	GRCGYC	2 cut(s)	189, 957
Hin1II	CATG	4 cut(s)	526, 1181, 1465, 1487
Hin6I	GCGC	6 cut(s)	291, 678, 864, 957, 1491, 1538
HinP1I	GCGC	6 cut(s)	291, 678, 864, 957, 1491, 1538
HincII	GTYRAC	2 cut(s)	774, 1324
HindII	GTYRAC	2 cut(s)	774, 1324
HpaII	CCGG	6 cut(s)	198, 615, 646, 1067, 1076, 1370
HphI	GGTGA	6 cut(s)	115, 635, 829, 841, 901, 1238
Hpy166II	GTNNAC	4 cut(s)	639, 774, 795, 1324
Hpy188I	TCNGA	6 cut(s)	239, 540, 580, 845, 1120, 1261
Hpy188III	TCNNGA	6 cut(s)	395, 487, 985, 1132, 1403, 1474
Hpy8I	GTNNAC	4 cut(s)	639, 774, 795, 1324
Hpy99I	CGWCG	5 cut(s)	191, 437, 1325, 1475, 1560
HpyCH4III	ACNGT	3 cut(s)	433, 724, 920
HpyCH4IV	ACGT	5 cut(s)	909, 1011, 1115, 1206, 1221
HpyCH4V	TGCA	8 cut(s)	423, 1177, 1238, 1418, 1526, 1577, 1594, 1631
HpyF10VI	GCNNNNNNNGC	7 cut(s)	270, 449, 704, 968, 997, 1071, 1488
HpyF3I	CTNAG	3 cut(s)	77, 992, 1146
HpySE526I	ACGT	5 cut(s)	909, 1011, 1115, 1206, 1221
Hsp92I	GRCGYC	2 cut(s)	189, 957
Hsp92II	CATG	4 cut(s)	526, 1181, 1465, 1487
HspAI	GCGC	6 cut(s)	291, 678, 864, 957, 1491, 1538
KasI	GGCGCC	1 cut(s)	956
Ksp22I	TGATCA	1 cut(s)	1341
Kzo9I	GATC	8 cut(s)	31, 86, 827, 945, 981, 1341, 1384, 1509
LguI	GCTCTTC	2 cut(s)	11, 968
LmnI	GCTCC	7 cut(s)	392, 703, 938, 967, 1097, 1141, 1479
Lsp1109I	GCAGC	5 cut(s)	33, 36, 938, 990, 1430
LweI	GCATC	4 cut(s)	681, 1164, 1225, 1405
MabI	ACCWGGT	1 cut(s)	1359
MaeI	CTAG	1 cut(s)	788
MaeII	ACGT	5 cut(s)	909, 1011, 1115, 1206, 1221
MaeIII	GTNAC	2 cut(s)	920, 1217
MalI	GATC	8 cut(s)	33, 88, 829, 947, 983, 1343, 1386, 1511
MbiI	CCGCTC	4 cut(s)	458, 698, 838, 962
MboI	GATC	8 cut(s)	31, 86, 827, 945, 981, 1341, 1384, 1509
MboII	GAAGA	5 cut(s)	28, 985, 1091, 1244, 1561
MflI	RGATCY	3 cut(s)	86, 827, 1384
MhlI	GDGCHC	2 cut(s)	935, 1076
MluCI	AATT	2 cut(s)	1185, 1337
MluI	ACGCGT	2 cut(s)	690, 1558
Mly113I	GGCGCC	1 cut(s)	957
MlyI	GAGTC	4 cut(s)	243, 497, 527, 1250
MmeI	TCCRAC	2 cut(s)	399, 1359
Mph1103I	ATGCAT	1 cut(s)	1179
MroXI	GAANNNNTTC	2 cut(s)	555, 1308
MseI	TTAA	1 cut(s)	1271
MslI	CAYNNNNRTG	3 cut(s)	527, 854, 908
MspA1I	CMGCKG	2 cut(s)	967, 1421
MspI	CCGG	6 cut(s)	198, 615, 646, 1067, 1076, 1370
MspR9I	CCNGG	5 cut(s)	616, 1067, 1076, 1361, 1370
Mva1269I	GAATGC	2 cut(s)	437, 1565
MvaI	CCWGG	1 cut(s)	1361
MvnI	CGCG	5 cut(s)	678, 680, 692, 752, 1560
MwoI	GCNNNNNNNGC	7 cut(s)	270, 449, 704, 968, 997, 1071, 1488
NarI	GGCGCC	1 cut(s)	957
NciI	CCSGG	4 cut(s)	616, 1067, 1076, 1370
NcoI	CCATGG	2 cut(s)	1461, 1483
NdeI	CATATG	1 cut(s)	419
NdeII	GATC	8 cut(s)	31, 86, 827, 945, 981, 1341, 1384, 1509
NheI	GCTAGC	1 cut(s)	787
NlaIII	CATG	4 cut(s)	526, 1181, 1465, 1487
NlaIV	GGNNCC	7 cut(s)	490, 610, 842, 958, 1074, 1143, 1481
NmeAIII	GCCGAG	5 cut(s)	249, 333, 485, 535, 1079
NsiI	ATGCAT	1 cut(s)	1179
NspV	TTCGAA	2 cut(s)	559, 685
OliI	CACNNNNGTG	1 cut(s)	854
PciSI	GCTCTTC	2 cut(s)	11, 968
PcsI	WCGNNNNNNNCGW	2 cut(s)	360, 507
PctI	GAATGC	2 cut(s)	437, 1565
PdmI	GAANNNNTTC	2 cut(s)	555, 1308
PfeI	GAWTC	6 cut(s)	551, 592, 846, 1329, 1426, 1502
Pfl23II	CGTACG	1 cut(s)	741
PflMI	CCANNNNNTGG	1 cut(s)	41
PleI	GAGTC	4 cut(s)	242, 497, 526, 1250
PluTI	GGCGCC	1 cut(s)	960
PpsI	GAGTC	4 cut(s)	242, 497, 526, 1250
Ppu21I	YACGTR	1 cut(s)	1012
Psp124BI	GAGCTC	1 cut(s)	935
Psp6I	CCWGG	1 cut(s)	1359
PspGI	CCWGG	1 cut(s)	1359
PspLI	CGTACG	1 cut(s)	741
PspN4I	GGNNCC	7 cut(s)	490, 610, 842, 958, 1074, 1143, 1481
PspOMI	GGGCCC	1 cut(s)	1072
PspPI	GGNCC	7 cut(s)	489, 609, 1051, 1064, 1072, 1073, 1199
PsuI	RGATCY	3 cut(s)	86, 827, 1384
PvuII	CAGCTG	1 cut(s)	1421
RsaI	GTAC	7 cut(s)	721, 743, 755, 1014, 1349, 1433, 1458
RsaNI	GTAC	7 cut(s)	720, 742, 754, 1013, 1348, 1432, 1457
RseI	CAYNNNNRTG	3 cut(s)	527, 854, 908
SacI	GAGCTC	1 cut(s)	935
SalI	GTCGAC	1 cut(s)	1322
SapI	GCTCTTC	2 cut(s)	11, 968
SaqAI	TTAA	1 cut(s)	1271
Sau3AI	GATC	8 cut(s)	31, 86, 827, 945, 981, 1341, 1384, 1509
Sau96I	GGNCC	7 cut(s)	489, 609, 1051, 1064, 1072, 1073, 1199
SchI	GAGTC	4 cut(s)	243, 497, 527, 1250
ScrFI	CCNGG	5 cut(s)	616, 1067, 1076, 1361, 1370
SduI	GDGCHC	2 cut(s)	935, 1076
SexAI	ACCWGGT	1 cut(s)	1359
SfaNI	GCATC	4 cut(s)	681, 1164, 1225, 1405
SfiI	GGCCNNNNNGGCC	1 cut(s)	1071
SfoI	GGCGCC	1 cut(s)	958
SfuI	TTCGAA	2 cut(s)	559, 685
SinI	GGWCC	3 cut(s)	489, 609, 1199
SmiMI	CAYNNNNRTG	3 cut(s)	527, 854, 908
Sse9I	AATT	2 cut(s)	1185, 1337
SspDI	GGCGCC	1 cut(s)	956
SspMI	CTAG	1 cut(s)	788
SstI	GAGCTC	1 cut(s)	935
StyD4I	CCNGG	5 cut(s)	614, 1065, 1074, 1359, 1368
StyI	CCWWGG	3 cut(s)	885, 1461, 1483
TaaI	ACNGT	3 cut(s)	433, 724, 920
TaiI	ACGT	5 cut(s)	912, 1014, 1118, 1209, 1224
TaqII	GACCGA	1 cut(s)	1341
TasI	AATT	2 cut(s)	1185, 1337
TatI	WGTACW	1 cut(s)	1347
TauI	GCSGC	5 cut(s)	194, 251, 957, 962, 1003
TfiI	GAWTC	6 cut(s)	551, 592, 846, 1329, 1426, 1502
Tru1I	TTAA	1 cut(s)	1271
Tru9I	TTAA	1 cut(s)	1271
TscAI	CASTG	2 cut(s)	454, 1162
TseI	GCWGC	5 cut(s)	21, 24, 951, 1003, 1418
TspDTI	ATGAA	7 cut(s)	214, 751, 1226, 1245, 1317, 1515, 1558
TspGWI	ACGGA	2 cut(s)	1055, 1419
TspRI	CASTG	2 cut(s)	454, 1162
Van91I	CCANNNNNTGG	1 cut(s)	41
VpaK11BI	GGWCC	3 cut(s)	489, 609, 1199
XapI	RAATTY	1 cut(s)	1185
XmiI	GTMKAC	1 cut(s)	1323
XmnI	GAANNNNTTC	2 cut(s)	555, 1308
XspI	CTAG	1 cut(s)	788
Zsp2I	ATGCAT	1 cut(s)	1179

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.