MD06G1185700.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005135
Physical Location & Seq
Reverse (-)
32354553 .. 32355997
1445 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1185700.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGCTCTCCATTCCACCACCTCCATTCCTCCTCCCCCTCCTCCTCCTCCTCCAACTCACCGCCACTCTCCTCCTCCTCCTCTTCAATTTCAAACACTGCACCACCCTCCTCTCCAATTGCTTTCGAGGTTTTTCGCGTTCCCGGCTCTCGGATTGGTTCCGATTGCGTTTTGGGATACGCAAAATGCCCCTTAAGGGCGCCACCCTCCAGGCCTGCGCCGCGTGCAAATACCAGCGAAGGAAGTGCACGGCGGAGTGCCCCTTGGCGCCCTATTTCCCGGCGGACCAACCCAAGATGTACCAAAACGCACATAAGCTATTCGGAGTCTGCAACATTTTGAAAATACTTAAAAATCTCAACCCTCGCCAGAAGGTGGAGGCCATGAGGTCCATAATATACCAAGCTAACTTGCGCGACAAGTTTCCGGTTTACGGGTGCTGGGAGGTGATCAGGCAGCTCCAGTACCAAATCCTGGTGGCGGAGGAAGAGCTCCAAGCTGTCCACCAACAGCTGGCAATGTACAGACAGCACCGCCACCACCAGATTTCGTCCATGCCGGAATATGTGACGTCACAGTTGGAGCTAGGGATGGCTCCGCCCAATAACCCCCTCGCACTTTTCGACTACAATCTTCCCCCTTATATTCTCACTAATACCAACAATGCCACCGCAGTGGCTCTTGTAAATCAACACCAACAGCAATCTTACTCTAGCAGCAGTGATGCTGCTTACAGCTCTGCTGCTTATAACAGTGACTCCAAAGACAACGTCAACAACAGCAACAACAATGCTGGAAATCCGTTGTGGAGTTCAAATCCTTTTATGACTAACAACAGCAACAACAACAACAATAATAACTCCGAGGCGATTGAATCTGAACTCGGAGCTCTTCAGCAGCTCTCTATCCAACCTGACGTTGTTCAAGATTACGATGAGTTGCATCCGTTTTTTGACACCACCGATGATAGACAGTCATACATTGATTCCAAAGAGGCTTATGATTCAAGCTCGGAAGAATCGTTCAAGGACACGACACAATCGATGGAGCATGCAGAGAATGCATTGAAGAATGATGCAGCATGCTTTAGTCTTACCAGTGTCAACTGA

Protein Analysis

369

Amino Acids

41.74

Weight (kDa)

6.14

Isoelectric Point (pI)

61.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 71 - 168 1.9e-35 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 747
AatII GACGTC 1 cut(s) 572
AccB1I GGYRCC 2 cut(s) 197, 265
AccB7I CCANNNNNTGG 3 cut(s) 373, 472, 511
AccII CGCG 3 cut(s) 136, 221, 414
AciI CCGC 8 cut(s) 60, 219, 251, 281, 479, 532, 596, 669
AcuI CTGAAG 1 cut(s) 875
AcyI GRCGYC 3 cut(s) 198, 266, 569
AfaI GTAC 3 cut(s) 299, 464, 521
AfiI CCNNNNNNNGG 7 cut(s) 148, 194, 373, 431, 472, 478, 511
AflII CTTAAG 1 cut(s) 191
AgsI TTSAA 9 cut(s) 85, 91, 340, 813, 872, 923, 1005, 1024, 1066
AjnI CCWGG 2 cut(s) 207, 471
AleI CACNNNNGTG 1 cut(s) 671
AloI GAACNNNNNNTCC 2 cut(s) 1004, 1036
Alw21I GWGCWC 3 cut(s) 248, 492, 889
Alw44I GTGCAC 1 cut(s) 244
AoxI GGCC 2 cut(s) 210, 378
ApaLI GTGCAC 1 cut(s) 244
ApeKI GCWGC 6 cut(s) 454, 714, 725, 740, 895, 1076
ArsI GACNNNNNNTTYG 2 cut(s) 753, 785
AspLEI GCGC 4 cut(s) 200, 218, 268, 414
AspS9I GGNCC 2 cut(s) 283, 387
AsuC2I CCSGG 2 cut(s) 142, 278
AsuHPI GGTGA 2 cut(s) 49, 457
AvaII GGWCC 2 cut(s) 283, 387
BaeGI GKGCMC 2 cut(s) 248, 260
BanI GGYRCC 2 cut(s) 197, 265
BanII GRGCYC 2 cut(s) 492, 889
Bbv12I GWGCWC 3 cut(s) 248, 492, 889
BbvI GCAGC 6 cut(s) 466, 712, 726, 727, 907, 1088
BccI CCATC 2 cut(s) 583, 1036
BceAI ACGGC 1 cut(s) 264
BcgI CGANNNNNNTGC 2 cut(s) 225, 259
BciT130I CCWGG 2 cut(s) 209, 473
BciVI GTATCC 1 cut(s) 168
BclI TGATCA 1 cut(s) 447
BcnI CCSGG 2 cut(s) 142, 278
BfaI CTAG 2 cut(s) 584, 711
BfoI RGCGCY 2 cut(s) 201, 269
BfrI CTTAAG 1 cut(s) 191
BfuI GTATCC 1 cut(s) 168
BisI GCNGC 7 cut(s) 219, 455, 715, 726, 741, 896, 1077
BlsI GCNGC 7 cut(s) 220, 456, 716, 727, 742, 897, 1078
Bme1390I CCNGG 4 cut(s) 142, 209, 278, 473
Bme18I GGWCC 2 cut(s) 283, 387
BmgT120I GGNCC 2 cut(s) 283, 387
BmiI GGNNCC 4 cut(s) 158, 199, 267, 594
BmrFI CCNGG 4 cut(s) 142, 209, 278, 473
BmsI GCATC 3 cut(s) 712, 949, 1063
BpmI CTGGAG 2 cut(s) 191, 443
BpuMI CCSGG 2 cut(s) 142, 278
Bsa29I ATCGAT 1 cut(s) 1040
BsaHI GRCGYC 3 cut(s) 198, 266, 569
BsaJI CCNNGG 2 cut(s) 261, 861
BsaWI WCCGGW 1 cut(s) 424
BsaXI ACNNNNNCTCC 2 cut(s) 740, 770
Bsc4I CCNNNNNNNGG 7 cut(s) 148, 194, 373, 431, 472, 478, 511
Bse1I ACTGG 2 cut(s) 460, 1095
Bse3DI GCAATG 1 cut(s) 522
BseBI CCWGG 2 cut(s) 209, 473
BseCI ATCGAT 1 cut(s) 1040
BseDI CCNNGG 2 cut(s) 261, 861
BseGI GGATG 2 cut(s) 594, 940
BseLI CCNNNNNNNGG 7 cut(s) 148, 194, 373, 431, 472, 478, 511
BseMI GCAATG 1 cut(s) 522
BseNI ACTGG 2 cut(s) 460, 1095
BseSI GKGCMC 2 cut(s) 248, 260
BseXI GCAGC 6 cut(s) 466, 712, 726, 727, 907, 1088
BseYI CCCAGC 1 cut(s) 438
BsgI GTGCAG 1 cut(s) 82
Bsh1236I CGCG 3 cut(s) 136, 221, 414
BshFI GGCC 2 cut(s) 212, 380
BshNI GGYRCC 2 cut(s) 197, 265
BshVI ATCGAT 1 cut(s) 1040
BsiHKAI GWGCWC 3 cut(s) 248, 492, 889
BsiSI CCGG 4 cut(s) 142, 278, 425, 557
BslI CCNNNNNNNGG 7 cut(s) 148, 194, 373, 431, 472, 478, 511
BsmI GAATGC 1 cut(s) 1063
BsnI GGCC 2 cut(s) 212, 380
Bsp1286I GDGCHC 4 cut(s) 248, 260, 492, 889
Bsp1407I TGTACA 1 cut(s) 519
Bsp143I GATC 1 cut(s) 447
BspACI CCGC 8 cut(s) 60, 219, 251, 281, 479, 532, 596, 669
BspANI GGCC 2 cut(s) 212, 380
BspDI ATCGAT 1 cut(s) 1040
BspFNI CGCG 3 cut(s) 136, 221, 414
BspLI GGNNCC 4 cut(s) 158, 199, 267, 594
BspQI GCTCTTC 2 cut(s) 480, 894
BspT107I GGYRCC 2 cut(s) 197, 265
BspTI CTTAAG 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 522
BsrGI TGTACA 1 cut(s) 519
BsrI ACTGG 2 cut(s) 460, 1095
BssECI CCNNGG 2 cut(s) 261, 861
BssMI GATC 1 cut(s) 447
BssNI GRCGYC 3 cut(s) 198, 266, 569
BssT1I CCWWGG 1 cut(s) 261
Bst2UI CCWGG 2 cut(s) 209, 473
Bst4CI ACNGT 3 cut(s) 576, 752, 972
Bst6I CTCTTC 3 cut(s) 86, 480, 894
BstACI GRCGYC 3 cut(s) 198, 266, 569
BstAFI CTTAAG 1 cut(s) 191
BstAPI GCANNNNNTGC 1 cut(s) 1058
BstAUI TGTACA 1 cut(s) 519
BstC8I GCNNGC 5 cut(s) 214, 223, 513, 1050, 1081
BstF5I GGATG 2 cut(s) 594, 940
BstFNI CGCG 3 cut(s) 136, 221, 414
BstH2I RGCGCY 2 cut(s) 201, 269
BstHHI GCGC 4 cut(s) 200, 218, 268, 414
BstKTI GATC 1 cut(s) 450
BstMBI GATC 1 cut(s) 447
BstMWI GCNNNNNNNGC 4 cut(s) 142, 218, 222, 1058
BstNI CCWGG 2 cut(s) 209, 473
BstNSI RCATGY 2 cut(s) 1052, 1083
BstSCI CCNGG 4 cut(s) 140, 207, 276, 471
BstSLI GKGCMC 2 cut(s) 248, 260
BstUI CGCG 3 cut(s) 136, 221, 414
BstV1I GCAGC 6 cut(s) 466, 712, 726, 727, 907, 1088
BstXI CCANNNNNNTGG 1 cut(s) 673
Bsu15I ATCGAT 1 cut(s) 1040
BsuI GTATCC 1 cut(s) 168
BsuRI GGCC 2 cut(s) 212, 380
BsuTUI ATCGAT 1 cut(s) 1040
BtsCI GGATG 2 cut(s) 594, 940
BtsI GCAGTG 3 cut(s) 94, 678, 724
BtsIMutI CAGTG 5 cut(s) 94, 678, 724, 757, 1102
Cac8I GCNNGC 5 cut(s) 214, 223, 513, 1050, 1081
CfoI GCGC 4 cut(s) 200, 218, 268, 414
Cfr13I GGNCC 2 cut(s) 283, 387
ClaI ATCGAT 1 cut(s) 1040
Csp6I GTAC 3 cut(s) 298, 463, 520
CviAII CATG 4 cut(s) 382, 553, 1049, 1080
CviQI GTAC 3 cut(s) 298, 463, 520
DinI GGCGCC 2 cut(s) 199, 267
DpnI GATC 1 cut(s) 449
DpnII GATC 1 cut(s) 447
Eam1104I CTCTTC 3 cut(s) 86, 480, 894
EarI CTCTTC 3 cut(s) 86, 480, 894
EciI GGCGGA 4 cut(s) 266, 296, 494, 585
Ecl136II GAGCTC 2 cut(s) 490, 887
Eco130I CCWWGG 1 cut(s) 261
Eco147I AGGCCT 1 cut(s) 212
Eco24I GRGCYC 2 cut(s) 492, 889
Eco47I GGWCC 2 cut(s) 283, 387
Eco53kI GAGCTC 2 cut(s) 490, 887
Eco57I CTGAAG 1 cut(s) 875
EcoICRI GAGCTC 2 cut(s) 490, 887
EcoRII CCWGG 2 cut(s) 207, 471
EcoT14I CCWWGG 1 cut(s) 261
EcoT22I ATGCAT 1 cut(s) 1063
EcoT38I GRGCYC 2 cut(s) 492, 889
EgeI GGCGCC 2 cut(s) 199, 267
EheI GGCGCC 2 cut(s) 199, 267
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 4 cut(s) 385, 556, 1052, 1083
FatI CATG 4 cut(s) 381, 552, 1048, 1079
FbaI TGATCA 1 cut(s) 447
Fnu4HI GCNGC 7 cut(s) 219, 455, 715, 726, 741, 896, 1077
FokI GGATG 2 cut(s) 601, 927
FriOI GRGCYC 2 cut(s) 492, 889
Fsp4HI GCNGC 7 cut(s) 219, 455, 715, 726, 741, 896, 1077
FspBI CTAG 2 cut(s) 584, 711
GlaI GCGC 4 cut(s) 199, 217, 267, 413
GluI GCNGC 7 cut(s) 219, 455, 715, 726, 741, 896, 1077
GsaI CCCAGC 1 cut(s) 442
GsuI CTGGAG 2 cut(s) 191, 443
HaeII RGCGCY 2 cut(s) 201, 269
HaeIII GGCC 2 cut(s) 212, 380
HapII CCGG 4 cut(s) 142, 278, 425, 557
HhaI GCGC 4 cut(s) 200, 218, 268, 414
Hin1I GRCGYC 3 cut(s) 198, 266, 569
Hin1II CATG 4 cut(s) 385, 556, 1052, 1083
Hin6I GCGC 4 cut(s) 198, 216, 266, 412
HinP1I GCGC 4 cut(s) 198, 216, 266, 412
HincII GTYRAC 2 cut(s) 772, 1102
HindII GTYRAC 2 cut(s) 772, 1102
HinfI GANTC 6 cut(s) 324, 755, 872, 983, 1001, 1016
HpaII CCGG 4 cut(s) 142, 278, 425, 557
HphI GGTGA 2 cut(s) 49, 457
Hpy166II GTNNAC 5 cut(s) 246, 430, 502, 772, 1102
Hpy188I TCNGA 7 cut(s) 151, 161, 323, 862, 877, 884, 1012
Hpy188III TCNNGA 1 cut(s) 923
Hpy8I GTNNAC 5 cut(s) 246, 430, 502, 772, 1102
HpyAV CCTTC 2 cut(s) 231, 364
HpyCH4III ACNGT 3 cut(s) 576, 752, 972
HpyCH4IV ACGT 3 cut(s) 569, 768, 915
HpyCH4V TGCA 8 cut(s) 99, 225, 246, 330, 940, 1052, 1061, 1076
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 218, 222, 1058
HpySE526I ACGT 3 cut(s) 569, 768, 915
Hsp92I GRCGYC 3 cut(s) 198, 266, 569
Hsp92II CATG 4 cut(s) 385, 556, 1052, 1083
HspAI GCGC 4 cut(s) 198, 216, 266, 412
KasI GGCGCC 2 cut(s) 197, 265
Ksp22I TGATCA 1 cut(s) 447
Kzo9I GATC 1 cut(s) 447
LguI GCTCTTC 2 cut(s) 480, 894
LmnI GCTCC 6 cut(s) 462, 495, 580, 598, 884, 1045
Lsp1109I GCAGC 6 cut(s) 466, 712, 726, 727, 907, 1088
LweI GCATC 3 cut(s) 712, 949, 1063
MaeI CTAG 2 cut(s) 584, 711
MaeII ACGT 3 cut(s) 569, 768, 915
MaeIII GTNAC 3 cut(s) 565, 570, 752
MalI GATC 1 cut(s) 449
MboI GATC 1 cut(s) 447
MboII GAAGA 6 cut(s) 73, 497, 623, 881, 1025, 1078
MfeI CAATTG 1 cut(s) 115
MhlI GDGCHC 4 cut(s) 248, 260, 492, 889
MluCI AATT 2 cut(s) 85, 115
Mly113I GGCGCC 2 cut(s) 198, 266
MlyI GAGTC 2 cut(s) 333, 749
MmeI TCCRAC 3 cut(s) 76, 558, 931
Mph1103I ATGCAT 1 cut(s) 1063
MseI TTAA 2 cut(s) 192, 348
MslI CAYNNNNRTG 1 cut(s) 671
MspA1I CMGCKG 1 cut(s) 511
MspCI CTTAAG 1 cut(s) 191
MspI CCGG 4 cut(s) 142, 278, 425, 557
MspR9I CCNGG 4 cut(s) 142, 209, 278, 473
MunI CAATTG 1 cut(s) 115
Mva1269I GAATGC 1 cut(s) 1063
MvaI CCWGG 2 cut(s) 209, 473
MvnI CGCG 3 cut(s) 136, 221, 414
MwoI GCNNNNNNNGC 4 cut(s) 142, 218, 222, 1058
NarI GGCGCC 2 cut(s) 198, 266
NciI CCSGG 2 cut(s) 142, 278
NdeII GATC 1 cut(s) 447
NlaIII CATG 4 cut(s) 385, 556, 1052, 1083
NlaIV GGNNCC 4 cut(s) 158, 199, 267, 594
NmuCI GTSAC 3 cut(s) 565, 570, 752
NsiI ATGCAT 1 cut(s) 1063
NspI RCATGY 2 cut(s) 1052, 1083
OliI CACNNNNGTG 1 cut(s) 671
PaeI GCATGC 2 cut(s) 1052, 1083
PceI AGGCCT 1 cut(s) 212
PciSI GCTCTTC 2 cut(s) 480, 894
PcsI WCGNNNNNNNCGW 3 cut(s) 618, 1016, 1037
PctI GAATGC 1 cut(s) 1063
PfeI GAWTC 4 cut(s) 872, 983, 1001, 1016
PflFI GACNNNGTC 1 cut(s) 767
PflMI CCANNNNNTGG 3 cut(s) 373, 472, 511
PkrI GCNGC 7 cut(s) 220, 456, 716, 727, 742, 897, 1078
PleI GAGTC 2 cut(s) 332, 749
PluTI GGCGCC 2 cut(s) 201, 269
PpsI GAGTC 2 cut(s) 332, 749
PsiI TTATAA 1 cut(s) 747
Psp124BI GAGCTC 2 cut(s) 492, 889
Psp6I CCWGG 2 cut(s) 207, 471
PspFI CCCAGC 1 cut(s) 438
PspGI CCWGG 2 cut(s) 207, 471
PspN4I GGNNCC 4 cut(s) 158, 199, 267, 594
PspPI GGNCC 2 cut(s) 283, 387
PsyI GACNNNGTC 1 cut(s) 767
PvuII CAGCTG 1 cut(s) 511
RsaI GTAC 3 cut(s) 299, 464, 521
RsaNI GTAC 3 cut(s) 298, 463, 520
RseI CAYNNNNRTG 1 cut(s) 671
SacI GAGCTC 2 cut(s) 492, 889
SapI GCTCTTC 2 cut(s) 480, 894
SaqAI TTAA 2 cut(s) 192, 348
SatI GCNGC 7 cut(s) 219, 455, 715, 726, 741, 896, 1077
Sau3AI GATC 1 cut(s) 447
Sau96I GGNCC 2 cut(s) 283, 387
SchI GAGTC 2 cut(s) 333, 749
ScrFI CCNGG 4 cut(s) 142, 209, 278, 473
SduI GDGCHC 4 cut(s) 248, 260, 492, 889
SfaNI GCATC 3 cut(s) 712, 949, 1063
SfoI GGCGCC 2 cut(s) 199, 267
SinI GGWCC 2 cut(s) 283, 387
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
SphI GCATGC 2 cut(s) 1052, 1083
Sse9I AATT 2 cut(s) 85, 115
SseBI AGGCCT 1 cut(s) 212
SsiI CCGC 8 cut(s) 60, 219, 251, 281, 479, 532, 596, 669
SspDI GGCGCC 2 cut(s) 197, 265
SspMI CTAG 2 cut(s) 584, 711
SstI GAGCTC 2 cut(s) 492, 889
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 4 cut(s) 140, 207, 276, 471
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 3 cut(s) 576, 752, 972
TaiI ACGT 3 cut(s) 572, 771, 918
TaqI TCGA 3 cut(s) 124, 621, 1040
TasI AATT 2 cut(s) 85, 115
TatI WGTACW 1 cut(s) 519
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 4 cut(s) 872, 983, 1001, 1016
Tru1I TTAA 2 cut(s) 192, 348
Tru9I TTAA 2 cut(s) 192, 348
TscAI CASTG 5 cut(s) 101, 678, 724, 757, 1102
TseFI GTSAC 3 cut(s) 565, 570, 752
TseI GCWGC 6 cut(s) 454, 714, 725, 740, 895, 1076
Tsp45I GTSAC 3 cut(s) 565, 570, 752
TspGWI ACGGA 2 cut(s) 789, 933
TspRI CASTG 5 cut(s) 101, 678, 724, 757, 1102
Tth111I GACNNNGTC 1 cut(s) 767
Van91I CCANNNNNTGG 3 cut(s) 373, 472, 511
Vha464I CTTAAG 1 cut(s) 191
VneI GTGCAC 1 cut(s) 244
VpaK11BI GGWCC 2 cut(s) 283, 387
XceI RCATGY 2 cut(s) 1052, 1083
XspI CTAG 2 cut(s) 584, 711
ZraI GACGTC 1 cut(s) 570
Zsp2I ATGCAT 1 cut(s) 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.