pycom14g15960

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005135
Physical Location & Seq
Reverse (-)
18405752 .. 18407000
1249 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g15960.1

Sequence Viewer

Length: 1119 bp
ATGTTCCCCATATCACCACTTCCATTTCTCCTCCCCCTCCTCCTCCTCCAACTCACCGCCACTCTCCTCCTCATCCTCTTCAATCTCAACCACTGCATCACTCTCCTCTCCAACTGCTTTCGAGGTTTTTCGCCTTTCCGGCTCTCTGATTGGTTCCAATCTCATTTTGGGATCGAAAAAATGACCCTGAAGGGCACCACCAGCCAGGCCTGCGCCGCGTGCAAGTACCAGCGAAGGAAGTGCAGGGCGGAGTGCCCCTTGGCTCCCTATTTCCCGCCGGACCAACCCAAAATGTTCCAAAACGCACACAAGCTTTTCGGAGTCAGCAACATTTTGAAAATACTTAAAAATCTCGAACCTCGCCAGAAGCTAGAGGCCATGAGGTCCATCATATACCAAGCTAACGTGCGCGACAAGTTTCCGGTCTACGGGTGCTGGGAGGTGATCTGGCAGATCCAGTACCAAATCCTCGTGGCGGAGGAAGAGCTCCAAGCCGTTCATCAACAGCTGGCAGTGTACAGACAGCACCACCAGCACCAGCACCAGATTTTGTCCATGCCGGAATACGTGACGTCACACTTAGAGCTAGGGATGGCTCCGCCCAACAATCCCCTCCCACTTTTCAATCACAATCCTCCCCCTTATAATCACAATAATAACAACAATGCCACCGCGGTGGCTCCTATAGATCAGCACCAACAGCAGTCTTACTCTAACAGCAGTGATGCTGCCTACAGCTCTGCTGCTTATAATATTGACTCCAAAGACAACATCATCAACAACGACACCAACAACAATAATGATGCTGGAAATCAGATGTGGATTTCGAATCCTTTTATGACTAACAACAGCAACAACAACAATAATAATTCCGAGGCGATTCAATCCGAACTCGGAGTTCTTCAGCCGCTCTCTATCCAACCTGAAGTTGTTCAAGATTACGATGAGTTGCATCCGTTCTTTGACACCATCGATGATAGACAGTCATACATTGATTCCAAAGAGGCTTATGATTCTAGCTCGGAAGAATCGTTCAAGGACACAACACGGTCCATGGAGCATGCAGAGAATGCATTGAAGAACGCTGCAGCATGCTTTAGTCTAACCAGTGTCAACTGA

Protein Analysis

373

Amino Acids

42.42

Weight (kDa)

5.65

Isoelectric Point (pI)

59.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 70 - 167 8.9e-37 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 645, 750
AatII GACGTC 1 cut(s) 575
AccB1I GGYRCC 1 cut(s) 194
AccBSI CCGCTC 1 cut(s) 910
AccI GTMKAC 1 cut(s) 426
AccII CGCG 3 cut(s) 218, 411, 674
AciI CCGC 9 cut(s) 57, 216, 248, 275, 476, 599, 672, 674, 908
AclWI GGATC 2 cut(s) 179, 448
AcuI CTGAAG 3 cut(s) 209, 887, 945
AcyI GRCGYC 1 cut(s) 572
AfaI GTAC 3 cut(s) 227, 461, 518
AfiI CCNNNNNNNGG 2 cut(s) 428, 475
AgsI TTSAA 7 cut(s) 82, 337, 625, 884, 935, 1036, 1078
AjnI CCWGG 1 cut(s) 204
AleI CACNNNNGTG 1 cut(s) 674
AloI GAACNNNNNNTCC 2 cut(s) 1016, 1048
AluBI AGCT 8 cut(s) 313, 370, 401, 487, 508, 586, 738, 1020
AluI AGCT 8 cut(s) 313, 370, 401, 487, 508, 586, 738, 1020
Alw21I GWGCWC 1 cut(s) 489
AlwI GGATC 2 cut(s) 179, 448
AoxI GGCC 2 cut(s) 207, 375
ApeKI GCWGC 4 cut(s) 728, 743, 1085, 1088
Asp700I GAANNNNTTC 1 cut(s) 930
AspLEI GCGC 2 cut(s) 215, 411
AspS9I GGNCC 3 cut(s) 280, 384, 1050
AsuHPI GGTGA 3 cut(s) 6, 46, 454
AsuII TTCGAA 1 cut(s) 827
AvaII GGWCC 3 cut(s) 280, 384, 1050
BaeGI GKGCMC 2 cut(s) 197, 257
BanI GGYRCC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 489
BauI CACGAG 1 cut(s) 470
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 4 cut(s) 715, 730, 1072, 1100
BccI CCATC 3 cut(s) 395, 586, 977
BceAI ACGGC 1 cut(s) 479
BcgI CGANNNNNNTGC 2 cut(s) 222, 256
BciT130I CCWGG 1 cut(s) 206
BfaI CTAG 3 cut(s) 371, 587, 1017
BfmI CTRYAG 3 cut(s) 684, 733, 1086
BglI GCCNNNNNGGC 1 cut(s) 139
BisI GCNGC 6 cut(s) 216, 729, 744, 908, 1086, 1089
BlsI GCNGC 6 cut(s) 217, 730, 745, 909, 1087, 1090
Bme1390I CCNGG 1 cut(s) 206
Bme18I GGWCC 3 cut(s) 280, 384, 1050
BmgT120I GGNCC 3 cut(s) 280, 384, 1050
BmiI GGNNCC 5 cut(s) 155, 196, 264, 597, 681
BmrFI CCNGG 1 cut(s) 206
BmsI GCATC 4 cut(s) 105, 715, 793, 961
Bpu14I TTCGAA 1 cut(s) 827
Bsa29I ATCGAT 1 cut(s) 972
BsaAI YACGTR 1 cut(s) 568
BsaHI GRCGYC 1 cut(s) 572
BsaJI CCNNGG 4 cut(s) 258, 672, 873, 1053
BsaWI WCCGGW 1 cut(s) 421
Bsc4I CCNNNNNNNGG 2 cut(s) 428, 475
Bse1I ACTGG 2 cut(s) 457, 1107
BseBI CCWGG 1 cut(s) 206
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 4 cut(s) 258, 672, 873, 1053
BseGI GGATG 3 cut(s) 72, 597, 952
BseLI CCNNNNNNNGG 2 cut(s) 428, 475
BseNI ACTGG 2 cut(s) 457, 1107
BseRI GAGGAG 7 cut(s) 20, 29, 32, 35, 56, 59, 95
BseSI GKGCMC 2 cut(s) 197, 257
BseXI GCAGC 4 cut(s) 715, 730, 1072, 1100
BseYI CCCAGC 1 cut(s) 435
BsgI GTGCAG 1 cut(s) 262
Bsh1236I CGCG 3 cut(s) 218, 411, 674
BshFI GGCC 2 cut(s) 209, 377
BshNI GGYRCC 1 cut(s) 194
BshVI ATCGAT 1 cut(s) 972
BsiHKAI GWGCWC 1 cut(s) 489
BsiSI CCGG 4 cut(s) 139, 278, 422, 560
BslI CCNNNNNNNGG 2 cut(s) 428, 475
BsmI GAATGC 1 cut(s) 1075
BsnI GGCC 2 cut(s) 209, 377
Bsp119I TTCGAA 1 cut(s) 827
Bsp1286I GDGCHC 3 cut(s) 197, 257, 489
Bsp1407I TGTACA 1 cut(s) 516
Bsp143I GATC 4 cut(s) 171, 444, 453, 688
Bsp19I CCATGG 1 cut(s) 1053
BspACI CCGC 9 cut(s) 57, 216, 248, 275, 476, 599, 672, 674, 908
BspANI GGCC 2 cut(s) 209, 377
BspDI ATCGAT 1 cut(s) 972
BspFNI CGCG 3 cut(s) 218, 411, 674
BspLI GGNNCC 5 cut(s) 155, 196, 264, 597, 681
BspMAI CTGCAG 1 cut(s) 1090
BspPI GGATC 2 cut(s) 179, 448
BspQI GCTCTTC 1 cut(s) 477
BspT104I TTCGAA 1 cut(s) 827
BspT107I GGYRCC 1 cut(s) 194
BsrBI CCGCTC 1 cut(s) 910
BsrGI TGTACA 1 cut(s) 516
BsrI ACTGG 2 cut(s) 457, 1107
BssECI CCNNGG 4 cut(s) 258, 672, 873, 1053
BssMI GATC 4 cut(s) 171, 444, 453, 688
BssNI GRCGYC 1 cut(s) 572
BssSI CACGAG 1 cut(s) 470
BssT1I CCWWGG 2 cut(s) 258, 1053
Bst2BI CACGAG 1 cut(s) 470
Bst2UI CCWGG 1 cut(s) 206
Bst4CI ACNGT 2 cut(s) 984, 1050
Bst6I CTCTTC 2 cut(s) 83, 477
BstACI GRCGYC 1 cut(s) 572
BstAPI GCANNNNNTGC 1 cut(s) 1070
BstAUI TGTACA 1 cut(s) 516
BstBAI YACGTR 1 cut(s) 568
BstBI TTCGAA 1 cut(s) 827
BstC8I GCNNGC 5 cut(s) 211, 220, 510, 1062, 1093
BstDEI CTNAG 1 cut(s) 580
BstDSI CCRYGG 2 cut(s) 672, 1053
BstF5I GGATG 3 cut(s) 72, 597, 952
BstFNI CGCG 3 cut(s) 218, 411, 674
BstHHI GCGC 2 cut(s) 215, 411
BstKTI GATC 4 cut(s) 174, 447, 456, 691
BstMBI GATC 4 cut(s) 171, 444, 453, 688
BstMWI GCNNNNNNNGC 8 cut(s) 139, 201, 210, 215, 219, 532, 700, 1070
BstNI CCWGG 1 cut(s) 206
BstNSI RCATGY 2 cut(s) 1064, 1095
BstSCI CCNGG 1 cut(s) 204
BstSFI CTRYAG 3 cut(s) 684, 733, 1086
BstSLI GKGCMC 2 cut(s) 197, 257
BstUI CGCG 3 cut(s) 218, 411, 674
BstV1I GCAGC 4 cut(s) 715, 730, 1072, 1100
BstX2I RGATCY 1 cut(s) 453
BstXI CCANNNNNNTGG 1 cut(s) 676
BstYI RGATCY 1 cut(s) 453
Bsu15I ATCGAT 1 cut(s) 972
BsuRI GGCC 2 cut(s) 209, 377
BsuTUI ATCGAT 1 cut(s) 972
BtgI CCRYGG 2 cut(s) 672, 1053
BtsCI GGATG 3 cut(s) 72, 597, 952
BtsI GCAGTG 3 cut(s) 91, 519, 727
BtsIMutI CAGTG 4 cut(s) 91, 519, 727, 1114
Cac8I GCNNGC 5 cut(s) 211, 220, 510, 1062, 1093
CfoI GCGC 2 cut(s) 215, 411
Cfr13I GGNCC 3 cut(s) 280, 384, 1050
Cfr42I CCGCGG 1 cut(s) 675
ClaI ATCGAT 1 cut(s) 972
Csp6I GTAC 3 cut(s) 226, 460, 517
CviAII CATG 5 cut(s) 379, 556, 1054, 1061, 1092
CviQI GTAC 3 cut(s) 226, 460, 517
DdeI CTNAG 1 cut(s) 580
DpnI GATC 4 cut(s) 173, 446, 455, 690
DpnII GATC 4 cut(s) 171, 444, 453, 688
Eam1104I CTCTTC 2 cut(s) 83, 477
EarI CTCTTC 2 cut(s) 83, 477
EciI GGCGGA 3 cut(s) 263, 491, 588
Ecl136II GAGCTC 1 cut(s) 487
Eco130I CCWWGG 2 cut(s) 258, 1053
Eco147I AGGCCT 1 cut(s) 209
Eco24I GRGCYC 1 cut(s) 489
Eco47I GGWCC 3 cut(s) 280, 384, 1050
Eco53kI GAGCTC 1 cut(s) 487
Eco57I CTGAAG 3 cut(s) 209, 887, 945
EcoICRI GAGCTC 1 cut(s) 487
EcoRII CCWGG 1 cut(s) 204
EcoT14I CCWWGG 2 cut(s) 258, 1053
EcoT22I ATGCAT 1 cut(s) 1075
EcoT38I GRGCYC 1 cut(s) 489
ErhI CCWWGG 2 cut(s) 258, 1053
FaeI CATG 5 cut(s) 382, 559, 1057, 1064, 1095
FatI CATG 5 cut(s) 378, 555, 1053, 1060, 1091
FauI CCCGC 1 cut(s) 282
FblI GTMKAC 1 cut(s) 426
Fnu4HI GCNGC 6 cut(s) 216, 729, 744, 908, 1086, 1089
FokI GGATG 3 cut(s) 59, 604, 939
FriOI GRGCYC 1 cut(s) 489
Fsp4HI GCNGC 6 cut(s) 216, 729, 744, 908, 1086, 1089
FspBI CTAG 3 cut(s) 371, 587, 1017
GlaI GCGC 2 cut(s) 214, 410
GluI GCNGC 6 cut(s) 216, 729, 744, 908, 1086, 1089
GsaI CCCAGC 1 cut(s) 439
HaeIII GGCC 2 cut(s) 209, 377
HapII CCGG 4 cut(s) 139, 278, 422, 560
HhaI GCGC 2 cut(s) 215, 411
Hin1I GRCGYC 1 cut(s) 572
Hin1II CATG 5 cut(s) 382, 559, 1057, 1064, 1095
Hin6I GCGC 2 cut(s) 213, 409
HinP1I GCGC 2 cut(s) 213, 409
HincII GTYRAC 1 cut(s) 1114
HindII GTYRAC 1 cut(s) 1114
HindIII AAGCTT 1 cut(s) 311
HinfI GANTC 7 cut(s) 321, 758, 829, 880, 995, 1013, 1028
HpaII CCGG 4 cut(s) 139, 278, 422, 560
HphI GGTGA 3 cut(s) 6, 46, 454
Hpy166II GTNNAC 3 cut(s) 427, 517, 1114
Hpy188I TCNGA 7 cut(s) 148, 320, 816, 874, 889, 896, 1024
Hpy188III TCNNGA 2 cut(s) 353, 935
Hpy8I GTNNAC 3 cut(s) 427, 517, 1114
HpyAV CCTTC 2 cut(s) 184, 228
HpyCH4III ACNGT 2 cut(s) 984, 1050
HpyCH4IV ACGT 3 cut(s) 405, 567, 572
HpyCH4V TGCA 7 cut(s) 96, 222, 243, 952, 1064, 1073, 1088
HpyF10VI GCNNNNNNNGC 8 cut(s) 139, 201, 210, 215, 219, 532, 700, 1070
HpyF3I CTNAG 1 cut(s) 580
HpySE526I ACGT 3 cut(s) 405, 567, 572
Hsp92I GRCGYC 1 cut(s) 572
Hsp92II CATG 5 cut(s) 382, 559, 1057, 1064, 1095
HspAI GCGC 2 cut(s) 213, 409
KspI CCGCGG 1 cut(s) 675
Kzo9I GATC 4 cut(s) 171, 444, 453, 688
LguI GCTCTTC 1 cut(s) 477
LmnI GCTCC 5 cut(s) 268, 492, 601, 685, 1057
Lsp1109I GCAGC 4 cut(s) 715, 730, 1072, 1100
LweI GCATC 4 cut(s) 105, 715, 793, 961
MaeI CTAG 3 cut(s) 371, 587, 1017
MaeII ACGT 3 cut(s) 405, 567, 572
MaeIII GTNAC 2 cut(s) 568, 573
MalI GATC 4 cut(s) 173, 446, 455, 690
MbiI CCGCTC 1 cut(s) 910
MboI GATC 4 cut(s) 171, 444, 453, 688
MboII GAAGA 5 cut(s) 70, 494, 893, 1037, 1090
MflI RGATCY 1 cut(s) 453
MhlI GDGCHC 3 cut(s) 197, 257, 489
MluCI AATT 1 cut(s) 868
MlyI GAGTC 2 cut(s) 330, 752
MmeI TCCRAC 3 cut(s) 73, 135, 943
Mph1103I ATGCAT 1 cut(s) 1075
MroXI GAANNNNTTC 1 cut(s) 930
MseI TTAA 1 cut(s) 345
MslI CAYNNNNRTG 1 cut(s) 674
MspA1I CMGCKG 2 cut(s) 508, 674
MspI CCGG 4 cut(s) 139, 278, 422, 560
MspR9I CCNGG 1 cut(s) 206
Mva1269I GAATGC 1 cut(s) 1075
MvaI CCWGG 1 cut(s) 206
MvnI CGCG 3 cut(s) 218, 411, 674
MwoI GCNNNNNNNGC 8 cut(s) 139, 201, 210, 215, 219, 532, 700, 1070
NcoI CCATGG 1 cut(s) 1053
NdeII GATC 4 cut(s) 171, 444, 453, 688
NlaIII CATG 5 cut(s) 382, 559, 1057, 1064, 1095
NlaIV GGNNCC 5 cut(s) 155, 196, 264, 597, 681
NmuCI GTSAC 2 cut(s) 568, 573
NsiI ATGCAT 1 cut(s) 1075
NspI RCATGY 2 cut(s) 1064, 1095
NspV TTCGAA 1 cut(s) 827
OliI CACNNNNGTG 1 cut(s) 674
PaeI GCATGC 2 cut(s) 1064, 1095
PceI AGGCCT 1 cut(s) 209
PciSI GCTCTTC 1 cut(s) 477
PcsI WCGNNNNNNNCGW 1 cut(s) 1028
PctI GAATGC 1 cut(s) 1075
PdmI GAANNNNTTC 1 cut(s) 930
PfeI GAWTC 5 cut(s) 829, 880, 995, 1013, 1028
PkrI GCNGC 6 cut(s) 217, 730, 745, 909, 1087, 1090
PleI GAGTC 2 cut(s) 329, 752
PpsI GAGTC 2 cut(s) 329, 752
Ppu21I YACGTR 1 cut(s) 568
PsiI TTATAA 2 cut(s) 645, 750
Psp124BI GAGCTC 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 204
PspFI CCCAGC 1 cut(s) 435
PspGI CCWGG 1 cut(s) 204
PspN4I GGNNCC 5 cut(s) 155, 196, 264, 597, 681
PspPI GGNCC 3 cut(s) 280, 384, 1050
PstI CTGCAG 1 cut(s) 1090
PsuI RGATCY 1 cut(s) 453
PvuII CAGCTG 1 cut(s) 508
RsaI GTAC 3 cut(s) 227, 461, 518
RsaNI GTAC 3 cut(s) 226, 460, 517
RseI CAYNNNNRTG 1 cut(s) 674
SacI GAGCTC 1 cut(s) 489
SacII CCGCGG 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 477
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 6 cut(s) 216, 729, 744, 908, 1086, 1089
Sau3AI GATC 4 cut(s) 171, 444, 453, 688
Sau96I GGNCC 3 cut(s) 280, 384, 1050
SchI GAGTC 2 cut(s) 330, 752
ScrFI CCNGG 1 cut(s) 206
SduI GDGCHC 3 cut(s) 197, 257, 489
SfaNI GCATC 4 cut(s) 105, 715, 793, 961
SfcI CTRYAG 3 cut(s) 684, 733, 1086
Sfr303I CCGCGG 1 cut(s) 675
SfuI TTCGAA 1 cut(s) 827
SgrBI CCGCGG 1 cut(s) 675
SinI GGWCC 3 cut(s) 280, 384, 1050
SmiMI CAYNNNNRTG 1 cut(s) 674
SphI GCATGC 2 cut(s) 1064, 1095
Sse9I AATT 1 cut(s) 868
SseBI AGGCCT 1 cut(s) 209
SsiI CCGC 9 cut(s) 57, 216, 248, 275, 476, 599, 672, 674, 908
SspI AATATT 1 cut(s) 754
SspMI CTAG 3 cut(s) 371, 587, 1017
SstI GAGCTC 1 cut(s) 489
StuI AGGCCT 1 cut(s) 209
StyD4I CCNGG 1 cut(s) 204
StyI CCWWGG 2 cut(s) 258, 1053
TaaI ACNGT 2 cut(s) 984, 1050
TaiI ACGT 3 cut(s) 408, 570, 575
TaqI TCGA 5 cut(s) 121, 174, 354, 827, 972
TasI AATT 1 cut(s) 868
TatI WGTACW 1 cut(s) 516
TauI GCSGC 2 cut(s) 218, 910
TfiI GAWTC 5 cut(s) 829, 880, 995, 1013, 1028
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 4 cut(s) 98, 519, 727, 1114
TseFI GTSAC 2 cut(s) 568, 573
TseI GCWGC 4 cut(s) 728, 743, 1085, 1088
Tsp45I GTSAC 2 cut(s) 568, 573
TspDTI ATGAA 1 cut(s) 488
TspGWI ACGGA 1 cut(s) 945
TspRI CASTG 4 cut(s) 98, 519, 727, 1114
VpaK11BI GGWCC 3 cut(s) 280, 384, 1050
XceI RCATGY 2 cut(s) 1064, 1095
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
XmiI GTMKAC 1 cut(s) 426
XmnI GAANNNNTTC 1 cut(s) 930
XspI CTAG 3 cut(s) 371, 587, 1017
ZraI GACGTC 1 cut(s) 573
Zsp2I ATGCAT 1 cut(s) 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.