MD14G1191900.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005135
Physical Location & Seq
Reverse (-)
28286244 .. 28287840
1597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1191900.v1.1.491

Sequence Viewer

Length: 1131 bp
ATGTTCCCCATATCACCACTTCCATTTCTCCTCCCCCTCCTCCTCCTCCAACTCACCGCCACTCTCCTCCTCCTCCTCCTCCTCATCCTCTTCAATCTCAACCACTGCATCACCCTCCTCTCCAACTGCTTTCGAGGTTTTTCGCCTTCCCGGCTCTCTGATTGGTTCCAATCTCATTTTGGTATCCAAAAAATGACCCTGAAGGGCACCACCAGCCAGGCCTGCGCCGCGTGCAAGTACCAGCGAAGGAAGTGCAGGGCGGAGTGCCCCTTGGCTCCCTATTTCCCGCCGGACCAACCCAAAATGTTCCAAAACGCACACAAGCTTTTCGGAGTCAGCAACATTTTGAAAATACTTAAAAATCTCGAACCTCGCCAGAAGCTGGAGGCCATGAGGTCCATCATATACCAAGCTAACGTGCGCGACAAGTTTCCGGTCTACGGGTGCTGGGAGGTGATCTGGCAGCTCCAGTACCAAATCCTTGTGGCGGAGGAAGAGCTCCAAGCTGTTCATCAACAGCTGGCAATGTACAGACAGCACCACCAGCAACAGCACCAGATTTCGTCCATGCCGGAATACGTGACGTCACAGTTAGAGCTAGGGATGGCTCCGCCCAACAATCCCCTCCCACTTTTCATTCACAATCCTCCCCCTTATAATCATAATAATAACAACAATGCCACCGCGGTGGCTCCAGTAAATCAATACCAACAGCAATCTTACTCTAACAGCAGTGATGCTGCTTACAGCTCTGCTGCTTATAATATTGACTCCAAAGACATCATCAACACCAACACCAATAACAATAATAATGATGCTGGAAATCCGATGTGGACTTCGAATCCTTTTATGACTAACAACAGCAACAACAACAATAATAATTCTGAGGCGATTCAATCGGAACTCGGAGTTCTTCAGCCGCTCTCTATCCAACCTGAAGTTGTTCAAGATTATGATGAGTTGCATCCGTTCTTTGACACCATCGATGATAGACAGTCATACATTGATTCCAAAGAGGCTTATGATTCAAGCTCGGAAGAATCGTTCAAGGACACAACACGATCCATGGAGCATGCAGAGAATGCATTGAAGAATGCTGCAGCATGCTTTAGTCTAACCAGTGTCAACTGA

Protein Analysis

377

Amino Acids

42.76

Weight (kDa)

5.75

Isoelectric Point (pI)

59.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 74 - 171 3.5e-37 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 657, 762
AatII GACGTC 1 cut(s) 587
AccB1I GGYRCC 1 cut(s) 206
AccB7I CCANNNNNTGG 1 cut(s) 382
AccBSI CCGCTC 1 cut(s) 922
AccI GTMKAC 1 cut(s) 438
AccII CGCG 3 cut(s) 230, 423, 686
AciI CCGC 9 cut(s) 57, 228, 260, 287, 488, 611, 684, 686, 920
AclWI GGATC 1 cut(s) 1056
AcuI CTGAAG 3 cut(s) 221, 899, 957
AcyI GRCGYC 1 cut(s) 584
AfaI GTAC 3 cut(s) 239, 473, 530
AfiI CCNNNNNNNGG 3 cut(s) 382, 440, 487
AgsI TTSAA 7 cut(s) 94, 349, 896, 947, 1029, 1048, 1090
AjnI CCWGG 1 cut(s) 216
AleI CACNNNNGTG 1 cut(s) 686
AloI GAACNNNNNNTCC 2 cut(s) 1028, 1060
Alw21I GWGCWC 1 cut(s) 501
AlwI GGATC 1 cut(s) 1056
AlwNI CAGNNNCTG 1 cut(s) 382
AoxI GGCC 2 cut(s) 219, 387
ApeKI GCWGC 5 cut(s) 463, 740, 755, 1097, 1100
Asp700I GAANNNNTTC 1 cut(s) 942
AspLEI GCGC 2 cut(s) 227, 423
AspS9I GGNCC 2 cut(s) 292, 396
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 4 cut(s) 6, 46, 103, 466
AsuII TTCGAA 1 cut(s) 839
AvaII GGWCC 2 cut(s) 292, 396
BaeGI GKGCMC 2 cut(s) 209, 269
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 1 cut(s) 501
Bbv12I GWGCWC 1 cut(s) 501
BbvI GCAGC 5 cut(s) 475, 727, 742, 1084, 1112
BccI CCATC 3 cut(s) 407, 598, 989
BcgI CGANNNNNNTGC 2 cut(s) 234, 268
BciT130I CCWGG 1 cut(s) 218
BciVI GTATCC 1 cut(s) 194
BcnI CCSGG 1 cut(s) 151
BfaI CTAG 1 cut(s) 599
BfmI CTRYAG 1 cut(s) 1098
BfuI GTATCC 1 cut(s) 194
BglI GCCNNNNNGGC 1 cut(s) 151
BisI GCNGC 7 cut(s) 228, 464, 741, 756, 920, 1098, 1101
BlsI GCNGC 7 cut(s) 229, 465, 742, 757, 921, 1099, 1102
Bme1390I CCNGG 2 cut(s) 151, 218
Bme18I GGWCC 2 cut(s) 292, 396
BmgT120I GGNCC 2 cut(s) 292, 396
BmiI GGNNCC 5 cut(s) 167, 208, 276, 609, 693
BmrFI CCNGG 2 cut(s) 151, 218
BmsI GCATC 4 cut(s) 117, 727, 805, 973
BpmI CTGGAG 3 cut(s) 404, 452, 678
Bpu14I TTCGAA 1 cut(s) 839
BpuMI CCSGG 1 cut(s) 151
Bsa29I ATCGAT 1 cut(s) 984
BsaAI YACGTR 1 cut(s) 580
BsaHI GRCGYC 1 cut(s) 584
BsaJI CCNNGG 3 cut(s) 270, 684, 1065
BsaWI WCCGGW 1 cut(s) 433
Bsc4I CCNNNNNNNGG 3 cut(s) 382, 440, 487
Bse1I ACTGG 3 cut(s) 469, 695, 1119
Bse3DI GCAATG 1 cut(s) 531
BseBI CCWGG 1 cut(s) 218
BseCI ATCGAT 1 cut(s) 984
BseDI CCNNGG 3 cut(s) 270, 684, 1065
BseGI GGATG 3 cut(s) 84, 609, 964
BseLI CCNNNNNNNGG 3 cut(s) 382, 440, 487
BseMI GCAATG 1 cut(s) 531
BseMII CTCAG 1 cut(s) 876
BseNI ACTGG 3 cut(s) 469, 695, 1119
BseSI GKGCMC 2 cut(s) 209, 269
BseXI GCAGC 5 cut(s) 475, 727, 742, 1084, 1112
BseYI CCCAGC 1 cut(s) 447
BsgI GTGCAG 1 cut(s) 274
Bsh1236I CGCG 3 cut(s) 230, 423, 686
BshFI GGCC 2 cut(s) 221, 389
BshNI GGYRCC 1 cut(s) 206
BshVI ATCGAT 1 cut(s) 984
BsiHKAI GWGCWC 1 cut(s) 501
BsiSI CCGG 4 cut(s) 151, 290, 434, 572
BslI CCNNNNNNNGG 3 cut(s) 382, 440, 487
BsmI GAATGC 2 cut(s) 1087, 1099
BsnI GGCC 2 cut(s) 221, 389
Bsp119I TTCGAA 1 cut(s) 839
Bsp1286I GDGCHC 3 cut(s) 209, 269, 501
Bsp1407I TGTACA 1 cut(s) 528
Bsp143I GATC 2 cut(s) 456, 1061
Bsp19I CCATGG 1 cut(s) 1065
BspACI CCGC 9 cut(s) 57, 228, 260, 287, 488, 611, 684, 686, 920
BspANI GGCC 2 cut(s) 221, 389
BspCNI CTCAG 1 cut(s) 877
BspDI ATCGAT 1 cut(s) 984
BspFNI CGCG 3 cut(s) 230, 423, 686
BspLI GGNNCC 5 cut(s) 167, 208, 276, 609, 693
BspMAI CTGCAG 1 cut(s) 1102
BspPI GGATC 1 cut(s) 1056
BspQI GCTCTTC 1 cut(s) 489
BspT104I TTCGAA 1 cut(s) 839
BspT107I GGYRCC 1 cut(s) 206
BsrBI CCGCTC 1 cut(s) 922
BsrDI GCAATG 1 cut(s) 531
BsrGI TGTACA 1 cut(s) 528
BsrI ACTGG 3 cut(s) 469, 695, 1119
BssECI CCNNGG 3 cut(s) 270, 684, 1065
BssMI GATC 2 cut(s) 456, 1061
BssNI GRCGYC 1 cut(s) 584
BssT1I CCWWGG 2 cut(s) 270, 1065
Bst2UI CCWGG 1 cut(s) 218
Bst4CI ACNGT 2 cut(s) 591, 996
Bst6I CTCTTC 2 cut(s) 95, 489
BstACI GRCGYC 1 cut(s) 584
BstAPI GCANNNNNTGC 1 cut(s) 1082
BstAUI TGTACA 1 cut(s) 528
BstBAI YACGTR 1 cut(s) 580
BstBI TTCGAA 1 cut(s) 839
BstC8I GCNNGC 5 cut(s) 223, 232, 522, 1074, 1105
BstDEI CTNAG 1 cut(s) 885
BstDSI CCRYGG 2 cut(s) 684, 1065
BstF5I GGATG 3 cut(s) 84, 609, 964
BstFNI CGCG 3 cut(s) 230, 423, 686
BstHHI GCGC 2 cut(s) 227, 423
BstKTI GATC 2 cut(s) 459, 1064
BstMBI GATC 2 cut(s) 456, 1061
BstMWI GCNNNNNNNGC 7 cut(s) 151, 213, 222, 227, 231, 544, 1082
BstNI CCWGG 1 cut(s) 218
BstNSI RCATGY 2 cut(s) 1076, 1107
BstSCI CCNGG 2 cut(s) 149, 216
BstSFI CTRYAG 1 cut(s) 1098
BstSLI GKGCMC 2 cut(s) 209, 269
BstUI CGCG 3 cut(s) 230, 423, 686
BstV1I GCAGC 5 cut(s) 475, 727, 742, 1084, 1112
BstXI CCANNNNNNTGG 1 cut(s) 688
Bsu15I ATCGAT 1 cut(s) 984
BsuI GTATCC 1 cut(s) 194
BsuRI GGCC 2 cut(s) 221, 389
BsuTUI ATCGAT 1 cut(s) 984
BtgI CCRYGG 2 cut(s) 684, 1065
BtsCI GGATG 3 cut(s) 84, 609, 964
BtsI GCAGTG 2 cut(s) 103, 739
BtsIMutI CAGTG 3 cut(s) 103, 739, 1126
Cac8I GCNNGC 5 cut(s) 223, 232, 522, 1074, 1105
CaiI CAGNNNCTG 1 cut(s) 382
CfoI GCGC 2 cut(s) 227, 423
Cfr13I GGNCC 2 cut(s) 292, 396
Cfr42I CCGCGG 1 cut(s) 687
ClaI ATCGAT 1 cut(s) 984
Csp6I GTAC 3 cut(s) 238, 472, 529
CviAII CATG 5 cut(s) 391, 568, 1066, 1073, 1104
CviQI GTAC 3 cut(s) 238, 472, 529
DdeI CTNAG 1 cut(s) 885
DpnI GATC 2 cut(s) 458, 1063
DpnII GATC 2 cut(s) 456, 1061
Eam1104I CTCTTC 2 cut(s) 95, 489
EarI CTCTTC 2 cut(s) 95, 489
EciI GGCGGA 3 cut(s) 275, 503, 600
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 2 cut(s) 270, 1065
Eco147I AGGCCT 1 cut(s) 221
Eco24I GRGCYC 1 cut(s) 501
Eco47I GGWCC 2 cut(s) 292, 396
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 3 cut(s) 221, 899, 957
EcoICRI GAGCTC 1 cut(s) 499
EcoRII CCWGG 1 cut(s) 216
EcoT14I CCWWGG 2 cut(s) 270, 1065
EcoT22I ATGCAT 1 cut(s) 1087
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 2 cut(s) 270, 1065
FaeI CATG 5 cut(s) 394, 571, 1069, 1076, 1107
FatI CATG 5 cut(s) 390, 567, 1065, 1072, 1103
FauI CCCGC 1 cut(s) 294
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 7 cut(s) 228, 464, 741, 756, 920, 1098, 1101
FokI GGATG 3 cut(s) 71, 616, 951
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 7 cut(s) 228, 464, 741, 756, 920, 1098, 1101
FspBI CTAG 1 cut(s) 599
GlaI GCGC 2 cut(s) 226, 422
GluI GCNGC 7 cut(s) 228, 464, 741, 756, 920, 1098, 1101
GsaI CCCAGC 1 cut(s) 451
GsuI CTGGAG 3 cut(s) 404, 452, 678
HaeIII GGCC 2 cut(s) 221, 389
HapII CCGG 4 cut(s) 151, 290, 434, 572
HhaI GCGC 2 cut(s) 227, 423
Hin1I GRCGYC 1 cut(s) 584
Hin1II CATG 5 cut(s) 394, 571, 1069, 1076, 1107
Hin6I GCGC 2 cut(s) 225, 421
HinP1I GCGC 2 cut(s) 225, 421
HincII GTYRAC 1 cut(s) 1126
HindII GTYRAC 1 cut(s) 1126
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 7 cut(s) 333, 770, 841, 892, 1007, 1025, 1040
HpaII CCGG 4 cut(s) 151, 290, 434, 572
HphI GGTGA 4 cut(s) 6, 46, 103, 466
Hpy166II GTNNAC 3 cut(s) 439, 834, 1126
Hpy188I TCNGA 7 cut(s) 160, 332, 828, 886, 901, 908, 1036
Hpy188III TCNNGA 2 cut(s) 365, 947
Hpy8I GTNNAC 3 cut(s) 439, 834, 1126
HpyAV CCTTC 3 cut(s) 156, 196, 240
HpyCH4III ACNGT 2 cut(s) 591, 996
HpyCH4IV ACGT 3 cut(s) 417, 579, 584
HpyCH4V TGCA 7 cut(s) 108, 234, 255, 964, 1076, 1085, 1100
HpyF10VI GCNNNNNNNGC 7 cut(s) 151, 213, 222, 227, 231, 544, 1082
HpyF3I CTNAG 1 cut(s) 885
HpySE526I ACGT 3 cut(s) 417, 579, 584
Hsp92I GRCGYC 1 cut(s) 584
Hsp92II CATG 5 cut(s) 394, 571, 1069, 1076, 1107
HspAI GCGC 2 cut(s) 225, 421
KspI CCGCGG 1 cut(s) 687
Kzo9I GATC 2 cut(s) 456, 1061
LguI GCTCTTC 1 cut(s) 489
LmnI GCTCC 6 cut(s) 280, 471, 504, 613, 697, 1069
Lsp1109I GCAGC 5 cut(s) 475, 727, 742, 1084, 1112
LweI GCATC 4 cut(s) 117, 727, 805, 973
MaeI CTAG 1 cut(s) 599
MaeII ACGT 3 cut(s) 417, 579, 584
MaeIII GTNAC 2 cut(s) 580, 585
MalI GATC 2 cut(s) 458, 1063
MbiI CCGCTC 1 cut(s) 922
MboI GATC 2 cut(s) 456, 1061
MboII GAAGA 5 cut(s) 82, 506, 905, 1049, 1102
MhlI GDGCHC 3 cut(s) 209, 269, 501
MluCI AATT 1 cut(s) 880
MlyI GAGTC 2 cut(s) 342, 764
MmeI TCCRAC 3 cut(s) 73, 147, 955
Mph1103I ATGCAT 1 cut(s) 1087
MroXI GAANNNNTTC 1 cut(s) 942
MseI TTAA 1 cut(s) 357
MslI CAYNNNNRTG 1 cut(s) 686
MspA1I CMGCKG 2 cut(s) 520, 686
MspI CCGG 4 cut(s) 151, 290, 434, 572
MspR9I CCNGG 2 cut(s) 151, 218
Mva1269I GAATGC 2 cut(s) 1087, 1099
MvaI CCWGG 1 cut(s) 218
MvnI CGCG 3 cut(s) 230, 423, 686
MwoI GCNNNNNNNGC 7 cut(s) 151, 213, 222, 227, 231, 544, 1082
NciI CCSGG 1 cut(s) 151
NcoI CCATGG 1 cut(s) 1065
NdeII GATC 2 cut(s) 456, 1061
NlaIII CATG 5 cut(s) 394, 571, 1069, 1076, 1107
NlaIV GGNNCC 5 cut(s) 167, 208, 276, 609, 693
NmuCI GTSAC 2 cut(s) 580, 585
NsiI ATGCAT 1 cut(s) 1087
NspI RCATGY 2 cut(s) 1076, 1107
NspV TTCGAA 1 cut(s) 839
OliI CACNNNNGTG 1 cut(s) 686
PaeI GCATGC 2 cut(s) 1076, 1107
PceI AGGCCT 1 cut(s) 221
PciSI GCTCTTC 1 cut(s) 489
PcsI WCGNNNNNNNCGW 1 cut(s) 1040
PctI GAATGC 2 cut(s) 1087, 1099
PdmI GAANNNNTTC 1 cut(s) 942
PfeI GAWTC 5 cut(s) 841, 892, 1007, 1025, 1040
PflMI CCANNNNNTGG 1 cut(s) 382
PkrI GCNGC 7 cut(s) 229, 465, 742, 757, 921, 1099, 1102
PleI GAGTC 2 cut(s) 341, 764
PpsI GAGTC 2 cut(s) 341, 764
Ppu21I YACGTR 1 cut(s) 580
PsiI TTATAA 2 cut(s) 657, 762
Psp124BI GAGCTC 1 cut(s) 501
Psp6I CCWGG 1 cut(s) 216
PspFI CCCAGC 1 cut(s) 447
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 5 cut(s) 167, 208, 276, 609, 693
PspPI GGNCC 2 cut(s) 292, 396
PstI CTGCAG 1 cut(s) 1102
PstNI CAGNNNCTG 1 cut(s) 382
PvuII CAGCTG 1 cut(s) 520
RsaI GTAC 3 cut(s) 239, 473, 530
RsaNI GTAC 3 cut(s) 238, 472, 529
RseI CAYNNNNRTG 1 cut(s) 686
SacI GAGCTC 1 cut(s) 501
SacII CCGCGG 1 cut(s) 687
SapI GCTCTTC 1 cut(s) 489
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 7 cut(s) 228, 464, 741, 756, 920, 1098, 1101
Sau3AI GATC 2 cut(s) 456, 1061
Sau96I GGNCC 2 cut(s) 292, 396
SchI GAGTC 2 cut(s) 342, 764
ScrFI CCNGG 2 cut(s) 151, 218
SduI GDGCHC 3 cut(s) 209, 269, 501
SfaNI GCATC 4 cut(s) 117, 727, 805, 973
SfcI CTRYAG 1 cut(s) 1098
Sfr303I CCGCGG 1 cut(s) 687
SfuI TTCGAA 1 cut(s) 839
SgrBI CCGCGG 1 cut(s) 687
SinI GGWCC 2 cut(s) 292, 396
SmiMI CAYNNNNRTG 1 cut(s) 686
SphI GCATGC 2 cut(s) 1076, 1107
Sse9I AATT 1 cut(s) 880
SseBI AGGCCT 1 cut(s) 221
SsiI CCGC 9 cut(s) 57, 228, 260, 287, 488, 611, 684, 686, 920
SspI AATATT 1 cut(s) 766
SspMI CTAG 1 cut(s) 599
SstI GAGCTC 1 cut(s) 501
StuI AGGCCT 1 cut(s) 221
StyD4I CCNGG 2 cut(s) 149, 216
StyI CCWWGG 2 cut(s) 270, 1065
TaaI ACNGT 2 cut(s) 591, 996
TaiI ACGT 3 cut(s) 420, 582, 587
TaqI TCGA 4 cut(s) 133, 366, 839, 984
TasI AATT 1 cut(s) 880
TatI WGTACW 1 cut(s) 528
TauI GCSGC 2 cut(s) 230, 922
TfiI GAWTC 5 cut(s) 841, 892, 1007, 1025, 1040
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 3 cut(s) 110, 739, 1126
TseFI GTSAC 2 cut(s) 580, 585
TseI GCWGC 5 cut(s) 463, 740, 755, 1097, 1100
Tsp45I GTSAC 2 cut(s) 580, 585
TspDTI ATGAA 2 cut(s) 500, 625
TspGWI ACGGA 1 cut(s) 957
TspRI CASTG 3 cut(s) 110, 739, 1126
Van91I CCANNNNNTGG 1 cut(s) 382
VpaK11BI GGWCC 2 cut(s) 292, 396
XceI RCATGY 2 cut(s) 1076, 1107
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 438
XmnI GAANNNNTTC 1 cut(s) 942
XspI CTAG 1 cut(s) 599
ZraI GACGTC 1 cut(s) 585
Zsp2I ATGCAT 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.