MD09G1045500.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005135
Physical Location & Seq
Reverse (-)
2960398 .. 2961501
1104 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1045500.v1.1.491

Sequence Viewer

Length: 924 bp
ATGATGATCACCAAAAAGGTTGATGAATGTACATTCTACCCTACAATGACGATAAAAGGAGGCACAAGCCAAGCCTGTGCTGCGTGCAAATACCAAAGACGAAAGTGTGCTAAGGACTGCGCTCTTGCTCCACACTTTCCGCCCGATCAACCGAAGCTGTTTCAAAACGCCCACCGATTGTACGGCGTTGCGAACATAATGAAGATTCTAAAGCAAGTTCATCCTGAGAAAAGGGAGGAGGCTATGAGATCGATCATATATGAATCCAATATGTGTGCCAAATTTCCAGTCACCGGATGTATGGGCATCATAAACCAATTGACTTTCCAGTTACAGCAAGCCCAAGAAGAGCTTCGATATGTGAGAACGCATCTTGCAATTTGCAAGGATCAATGTCAATATCGAATGCCTTCTTCCCCGCAGCACTACTCGTGTCCTTCATCGCAGTTGCAGCTAGGTATACCTACGAACCCCGATGTGGCGGCTCTGACACTTTATCAACAACATCAATATCCGTATCAATATGCTTCTGGTGGCAGTGGTGGAACGCCCTGGGTGGCTAATGAGTATCTTGCAAATGGACTTAATGGGGTTTATATTGACGAAAATGATAATATGGTAAAACCTCTTAGGTTTCAACATCCCTATTATGACGATAACAATGTCGAACAACTTATGGCTATTCAGTCCAATTTGGTTCCTTCACAAGCCTTTCCTATTCACCAAGAAATGGATGTTCCCCATGATTATGATGACATTCCATTTGATACAATTGCTGATGATCGACAATCGTATATCGAATCTAAAGAAGCCTGTGAGTCTAGTGCTGAATCATCGTTCAAGGGCACGCAATCGATCGAACATGTCTCACAGAATGATCTCAAGAGTGCGGCAGCATGCTTCAGTCTAACAAGTGTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

308

Amino Acids

34.66

Weight (kDa)

5.92

Isoelectric Point (pI)

59.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 25 - 121 1.8e-36 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 730
AccI GTMKAC 1 cut(s) 460
AciI CCGC 4 cut(s) 140, 419, 482, 890
AclWI GGATC 1 cut(s) 396
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 1 cut(s) 886
AfaI GTAC 2 cut(s) 31, 182
AfiI CCNNNNNNNGG 3 cut(s) 293, 478, 730
AflIII ACRYGT 1 cut(s) 862
AgsI TTSAA 3 cut(s) 164, 638, 841
AjnI CCWGG 1 cut(s) 551
AjuI GAANNNNNNNTTGG 2 cut(s) 336, 368
AluBI AGCT 3 cut(s) 157, 352, 454
AluI AGCT 3 cut(s) 157, 352, 454
Alw26I GTCTC 1 cut(s) 871
AlwI GGATC 1 cut(s) 396
ApeKI GCWGC 4 cut(s) 80, 421, 451, 893
ApoI RAATTY 1 cut(s) 281
ArsI GACNNNNNNTTYG 4 cut(s) 273, 305, 746, 778
Asp700I GAANNNNTTC 2 cut(s) 351, 409
AspLEI GCGC 1 cut(s) 122
AsuHPI GGTGA 2 cut(s) 283, 713
BaeGI GKGCMC 1 cut(s) 848
BauI CACGAG 1 cut(s) 430
BbvI GCAGC 4 cut(s) 67, 433, 463, 905
BceAI ACGGC 1 cut(s) 199
BciT130I CCWGG 1 cut(s) 553
BclI TGATCA 1 cut(s) 6
BcoDI GTCTC 1 cut(s) 871
BfaI CTAG 2 cut(s) 455, 822
BisI GCNGC 6 cut(s) 81, 422, 452, 483, 891, 894
BlsI GCNGC 6 cut(s) 82, 423, 453, 484, 892, 895
Bme1390I CCNGG 1 cut(s) 553
BmiI GGNNCC 1 cut(s) 699
BmrFI CCNGG 1 cut(s) 553
BmsI GCATC 2 cut(s) 315, 379
Bpu10I CCTNAGC 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 866
Bsa29I ATCGAT 2 cut(s) 251, 854
BsaJI CCNNGG 2 cut(s) 551, 552
BsaWI WCCGGW 1 cut(s) 293
Bsc4I CCNNNNNNNGG 3 cut(s) 293, 478, 730
Bse1I ACTGG 2 cut(s) 287, 328
BseBI CCWGG 1 cut(s) 553
BseCI ATCGAT 2 cut(s) 251, 854
BseDI CCNNGG 2 cut(s) 551, 552
BseGI GGATG 4 cut(s) 220, 302, 640, 739
BseLI CCNNNNNNNGG 3 cut(s) 293, 478, 730
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 2 cut(s) 287, 328
BseRI GAGGAG 1 cut(s) 251
BseSI GKGCMC 1 cut(s) 848
BseXI GCAGC 4 cut(s) 67, 433, 463, 905
Bsh1285I CGRYCG 1 cut(s) 858
BshVI ATCGAT 2 cut(s) 251, 854
BsiEI CGRYCG 1 cut(s) 858
BsiSI CCGG 1 cut(s) 294
BslI CCNNNNNNNGG 3 cut(s) 293, 478, 730
BsmAI GTCTC 1 cut(s) 871
BsmI GAATGC 1 cut(s) 411
Bsp1286I GDGCHC 1 cut(s) 848
Bsp1407I TGTACA 1 cut(s) 29
Bsp143I GATC 8 cut(s) 6, 145, 248, 252, 388, 781, 855, 877
BspACI CCGC 4 cut(s) 140, 419, 482, 890
BspCNI CTCAG 1 cut(s) 217
BspDI ATCGAT 2 cut(s) 251, 854
BspLI GGNNCC 1 cut(s) 699
BspPI GGATC 1 cut(s) 396
BspQI GCTCTTC 1 cut(s) 342
BsrGI TGTACA 1 cut(s) 29
BsrI ACTGG 2 cut(s) 287, 328
BssECI CCNNGG 2 cut(s) 551, 552
BssMI GATC 8 cut(s) 6, 145, 248, 252, 388, 781, 855, 877
BssNAI GTATAC 1 cut(s) 461
BssSI CACGAG 1 cut(s) 430
Bst1107I GTATAC 1 cut(s) 461
Bst2BI CACGAG 1 cut(s) 430
Bst2UI CCWGG 1 cut(s) 553
Bst6I CTCTTC 1 cut(s) 342
BstAUI TGTACA 1 cut(s) 29
BstC8I GCNNGC 4 cut(s) 85, 339, 848, 898
BstDEI CTNAG 3 cut(s) 111, 225, 629
BstF5I GGATG 4 cut(s) 220, 302, 640, 739
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 8 cut(s) 9, 148, 251, 255, 391, 784, 858, 880
BstMAI GTCTC 1 cut(s) 871
BstMBI GATC 8 cut(s) 6, 145, 248, 252, 388, 781, 855, 877
BstMCI CGRYCG 1 cut(s) 858
BstMWI GCNNNNNNNGC 2 cut(s) 80, 451
BstNI CCWGG 1 cut(s) 553
BstNSI RCATGY 2 cut(s) 866, 900
BstSCI CCNGG 1 cut(s) 551
BstSLI GKGCMC 1 cut(s) 848
BstV1I GCAGC 4 cut(s) 67, 433, 463, 905
BstZ17I GTATAC 1 cut(s) 461
Bsu15I ATCGAT 2 cut(s) 251, 854
BsuTUI ATCGAT 2 cut(s) 251, 854
BtgZI GCGATG 1 cut(s) 426
BtsCI GGATG 4 cut(s) 220, 302, 640, 739
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 1 cut(s) 544
Cac8I GCNNGC 4 cut(s) 85, 339, 848, 898
CfoI GCGC 1 cut(s) 122
ClaI ATCGAT 2 cut(s) 251, 854
Csp6I GTAC 2 cut(s) 30, 181
CviAII CATG 3 cut(s) 743, 863, 897
CviQI GTAC 2 cut(s) 30, 181
DdeI CTNAG 3 cut(s) 111, 225, 629
DpnI GATC 8 cut(s) 8, 147, 250, 254, 390, 783, 857, 879
DpnII GATC 8 cut(s) 6, 145, 248, 252, 388, 781, 855, 877
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
EciI GGCGGA 1 cut(s) 129
Eco57I CTGAAG 1 cut(s) 886
EcoRII CCWGG 1 cut(s) 551
FaeI CATG 3 cut(s) 746, 866, 900
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FatI CATG 3 cut(s) 742, 862, 896
FauI CCCGC 1 cut(s) 426
FbaI TGATCA 1 cut(s) 6
FblI GTMKAC 1 cut(s) 460
Fnu4HI GCNGC 6 cut(s) 81, 422, 452, 483, 891, 894
FokI GGATG 4 cut(s) 207, 309, 627, 746
Fsp4HI GCNGC 6 cut(s) 81, 422, 452, 483, 891, 894
FspBI CTAG 2 cut(s) 455, 822
GlaI GCGC 1 cut(s) 121
GluI GCNGC 6 cut(s) 81, 422, 452, 483, 891, 894
HapII CCGG 1 cut(s) 294
HhaI GCGC 1 cut(s) 122
Hin1II CATG 3 cut(s) 746, 866, 900
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HinfI GANTC 5 cut(s) 205, 263, 800, 818, 830
HpaII CCGG 1 cut(s) 294
HphI GGTGA 2 cut(s) 283, 713
Hpy166II GTNNAC 1 cut(s) 461
Hpy188I TCNGA 1 cut(s) 489
Hpy188III TCNNGA 2 cut(s) 224, 883
Hpy8I GTNNAC 1 cut(s) 461
HpyAV CCTTC 3 cut(s) 420, 447, 711
HpyCH4V TGCA 5 cut(s) 87, 377, 384, 451, 575
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 451
HpyF3I CTNAG 3 cut(s) 111, 225, 629
Hsp92II CATG 3 cut(s) 746, 866, 900
HspAI GCGC 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 6
Kzo9I GATC 8 cut(s) 6, 145, 248, 252, 388, 781, 855, 877
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 1 cut(s) 133
LpnPI CCDG 9 cut(s) 88, 237, 300, 307, 341, 516, 538, 565, 826
Lsp1109I GCAGC 4 cut(s) 67, 433, 463, 905
LweI GCATC 2 cut(s) 315, 379
MaeI CTAG 2 cut(s) 455, 822
MaeIII GTNAC 2 cut(s) 289, 330
MalI GATC 8 cut(s) 8, 147, 250, 254, 390, 783, 857, 879
MboI GATC 8 cut(s) 6, 145, 248, 252, 388, 781, 855, 877
MboII GAAGA 3 cut(s) 214, 359, 405
MfeI CAATTG 2 cut(s) 317, 771
MhlI GDGCHC 1 cut(s) 848
MluCI AATT 5 cut(s) 281, 317, 378, 691, 771
MlyI GAGTC 1 cut(s) 827
MnlI CCTC 4 cut(s) 53, 229, 232, 636
MroXI GAANNNNTTC 2 cut(s) 351, 409
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 1 cut(s) 747
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 553
MunI CAATTG 2 cut(s) 317, 771
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 1 cut(s) 553
MwoI GCNNNNNNNGC 2 cut(s) 80, 451
NdeII GATC 8 cut(s) 6, 145, 248, 252, 388, 781, 855, 877
NlaIII CATG 3 cut(s) 746, 866, 900
NlaIV GGNNCC 1 cut(s) 699
NmuCI GTSAC 1 cut(s) 289
NspI RCATGY 2 cut(s) 866, 900
PaeI GCATGC 1 cut(s) 900
PasI CCCWGGG 1 cut(s) 552
PciI ACATGT 1 cut(s) 862
PciSI GCTCTTC 1 cut(s) 342
PctI GAATGC 1 cut(s) 411
PdmI GAANNNNTTC 2 cut(s) 351, 409
PfeI GAWTC 4 cut(s) 205, 263, 800, 830
PflMI CCANNNNNTGG 1 cut(s) 730
PkrI GCNGC 6 cut(s) 82, 423, 453, 484, 892, 895
Ple19I CGATCG 1 cut(s) 858
PleI GAGTC 1 cut(s) 826
PpsI GAGTC 1 cut(s) 826
PscI ACATGT 1 cut(s) 862
Psp6I CCWGG 1 cut(s) 551
PspGI CCWGG 1 cut(s) 551
PspN4I GGNNCC 1 cut(s) 699
PvuI CGATCG 1 cut(s) 858
RsaI GTAC 2 cut(s) 31, 182
RsaNI GTAC 2 cut(s) 30, 181
RseI CAYNNNNRTG 1 cut(s) 747
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 6 cut(s) 81, 422, 452, 483, 891, 894
Sau3AI GATC 8 cut(s) 6, 145, 248, 252, 388, 781, 855, 877
SchI GAGTC 1 cut(s) 827
ScrFI CCNGG 1 cut(s) 553
SduI GDGCHC 1 cut(s) 848
SetI ASST 8 cut(s) 21, 159, 354, 456, 460, 466, 628, 635
SfaNI GCATC 2 cut(s) 315, 379
SmiMI CAYNNNNRTG 1 cut(s) 747
SmlI CTYRAG 1 cut(s) 881
SmoI CTYRAG 1 cut(s) 881
SphI GCATGC 1 cut(s) 900
Sse9I AATT 5 cut(s) 281, 317, 378, 691, 771
SsiI CCGC 4 cut(s) 140, 419, 482, 890
SspMI CTAG 2 cut(s) 455, 822
StyD4I CCNGG 1 cut(s) 551
TaqI TCGA 8 cut(s) 251, 355, 403, 666, 784, 798, 854, 858
TasI AATT 5 cut(s) 281, 317, 378, 691, 771
TatI WGTACW 1 cut(s) 29
TauI GCSGC 2 cut(s) 485, 893
TfiI GAWTC 4 cut(s) 205, 263, 800, 830
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 1 cut(s) 544
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 4 cut(s) 80, 421, 451, 893
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 5 cut(s) 39, 209, 215, 276, 429
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 1 cut(s) 544
Van91I CCANNNNNTGG 1 cut(s) 730
XapI RAATTY 1 cut(s) 281
XceI RCATGY 2 cut(s) 866, 900
XmiI GTMKAC 1 cut(s) 460
XmnI GAANNNNTTC 2 cut(s) 351, 409
XspI CTAG 2 cut(s) 455, 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.