Rw7G002750

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0005135
Physical Location & Seq
Reverse (-)
2251661 .. 2252704
1044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002750.1

Sequence Viewer

Length: 924 bp
ATGACCCTCAAGGGTGCCACCAGCCAGGCCTGTGCTGCATGCAAATACCAAAGAAGAAAGTGCACGCCAGAGTGCCCTTTAGCTCCTTATTTCCCTCCAGACCAACCAAAAATGTTTCAAAATGCACACAAGCTTTTTGGGGTCTGCAACATCTTGAAGATACTTAAAAGTGTCGACCCTTGTCTCAAGTTTGAGGCCATGAAGTCCATCATCTACCAAGCCAACATTCGAGACAAGTTTCCGGTCTACGGCTGTTGGGAGGTGATCTGGCAATTACAGTACCAGATTATGGTTGCAGAGCAGGAGCTTCAAAATGTTCATAAACAGCTTGCATTGTACAGACAACATCACTACCAGCAGCAGCAGCAGATTTCAGCTATGCCAGATTATGTGACCTCACAATTAGAATTGGGCATGGCACCACCAAATAATAATAATAATACCACCTCACTTTTACATCATAGTACTCCACAATCTTATGATAACAAAAATACTGCCATACCTCATGCTTTGCCTGTAACACAACAACAACAACAGCAGCAGCAGCAGCCGCCTTACTCTCATCGAAGCGACGATGCTTACACTTCTGCTTATGACATTGACTCCAAGGAAATATTACCGGGGAGAATCAGTATCAATGAGAACAACGATAATGCAGAGACAGTTCAATCCCAGTTCGGAGTTTCGCAGACACTAGCTATTGAATCAGAAGATGTTCAAGATTACGATGAGCTGCACCCATTTTTCGATACCATAGATGATAGACAGTCATACATTGATTCCAAAGAGGCTTATGATTCAAGCAGTTCGGAAGAATCTTTCAAGGGCACAACACAATTATCGATGGATCATGCGGCGGAGAACACATTGAAGAGTGCTGCAGCATGCTTCAGTCTTACCAGTGTCAAGTTATCAGTAAGCTAG

Protein Analysis

307

Amino Acids

34.82

Weight (kDa)

5.33

Isoelectric Point (pI)

66.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 10 - 107 3e-36 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 14, 418
AccB7I CCANNNNNTGG 1 cut(s) 289
AccI GTMKAC 2 cut(s) 174, 246
AciI CCGC 3 cut(s) 551, 854, 857
AclWI GGATC 1 cut(s) 855
AcuI CTGAAG 1 cut(s) 874
AfaI GTAC 3 cut(s) 281, 338, 466
AfiI CCNNNNNNNGG 2 cut(s) 248, 289
AgsI TTSAA 9 cut(s) 119, 157, 311, 668, 704, 719, 801, 823, 871
AjnI CCWGG 1 cut(s) 24
AjuI GAANNNNNNNTTGG 2 cut(s) 210, 242
AluBI AGCT 8 cut(s) 83, 133, 307, 328, 377, 698, 733, 921
AluI AGCT 8 cut(s) 83, 133, 307, 328, 377, 698, 733, 921
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 3 cut(s) 188, 225, 653
Alw44I GTGCAC 1 cut(s) 61
AlwI GGATC 1 cut(s) 855
AoxI GGCC 2 cut(s) 27, 195
ApaLI GTGCAC 1 cut(s) 61
Asp700I GAANNNNTTC 1 cut(s) 714
AsuC2I CCSGG 1 cut(s) 621
AsuHPI GGTGA 1 cut(s) 274
BaeGI GKGCMC 3 cut(s) 65, 77, 830
BanI GGYRCC 2 cut(s) 14, 418
Bbv12I GWGCWC 1 cut(s) 65
BccI CCATC 2 cut(s) 215, 838
BceAI ACGGC 1 cut(s) 265
BciT130I CCWGG 1 cut(s) 26
BcnI CCSGG 1 cut(s) 621
BcoDI GTCTC 3 cut(s) 188, 225, 653
BfaI CTAG 2 cut(s) 695, 922
BfmI CTRYAG 1 cut(s) 879
BmcAI AGTACT 1 cut(s) 466
Bme1390I CCNGG 2 cut(s) 26, 621
BmiI GGNNCC 2 cut(s) 16, 420
BmrFI CCNGG 2 cut(s) 26, 621
BmrI ACTGGG 1 cut(s) 667
BmsI GCATC 1 cut(s) 565
BmuI ACTGGG 1 cut(s) 667
BoxI GACNNNNGTC 1 cut(s) 180
BpmI CTGGAG 1 cut(s) 81
BpuEI CTTGAG 1 cut(s) 170
BpuMI CCSGG 1 cut(s) 621
Bsa29I ATCGAT 1 cut(s) 842
BsaJI CCNNGG 2 cut(s) 606, 620
BsaWI WCCGGW 1 cut(s) 241
BsaXI ACNNNNNCTCC 4 cut(s) 587, 616, 617, 646
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 289
Bse1I ACTGG 2 cut(s) 673, 900
BseBI CCWGG 1 cut(s) 26
BseCI ATCGAT 1 cut(s) 842
BseDI CCNNGG 2 cut(s) 606, 620
BseLI CCNNNNNNNGG 2 cut(s) 248, 289
BseNI ACTGG 2 cut(s) 673, 900
BseSI GKGCMC 3 cut(s) 65, 77, 830
BsgI GTGCAG 1 cut(s) 719
BshFI GGCC 2 cut(s) 29, 197
BshNI GGYRCC 2 cut(s) 14, 418
BshVI ATCGAT 1 cut(s) 842
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 2 cut(s) 242, 620
BslI CCNNNNNNNGG 2 cut(s) 248, 289
BsmAI GTCTC 3 cut(s) 188, 225, 653
BsnI GGCC 2 cut(s) 29, 197
Bsp1286I GDGCHC 3 cut(s) 65, 77, 830
Bsp1407I TGTACA 1 cut(s) 336
Bsp143I GATC 2 cut(s) 264, 847
BspACI CCGC 3 cut(s) 551, 854, 857
BspANI GGCC 2 cut(s) 29, 197
BspDI ATCGAT 1 cut(s) 842
BspLI GGNNCC 2 cut(s) 16, 420
BspMAI CTGCAG 1 cut(s) 883
BspPI GGATC 1 cut(s) 855
BspT107I GGYRCC 2 cut(s) 14, 418
BsrGI TGTACA 1 cut(s) 336
BsrI ACTGG 2 cut(s) 673, 900
BssECI CCNNGG 2 cut(s) 606, 620
BssMI GATC 2 cut(s) 264, 847
BssT1I CCWWGG 1 cut(s) 606
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 3 cut(s) 279, 664, 768
Bst6I CTCTTC 1 cut(s) 866
BstAUI TGTACA 1 cut(s) 336
BstC8I GCNNGC 4 cut(s) 40, 65, 330, 886
BstKTI GATC 2 cut(s) 267, 850
BstMAI GTCTC 3 cut(s) 188, 225, 653
BstMBI GATC 2 cut(s) 264, 847
BstMWI GCNNNNNNNGC 5 cut(s) 35, 364, 544, 547, 550
BstNI CCWGG 1 cut(s) 26
BstNSI RCATGY 2 cut(s) 42, 888
BstPAI GACNNNNGTC 1 cut(s) 180
BstSCI CCNGG 2 cut(s) 24, 619
BstSFI CTRYAG 1 cut(s) 879
BstSLI GKGCMC 3 cut(s) 65, 77, 830
Bsu15I ATCGAT 1 cut(s) 842
BsuRI GGCC 2 cut(s) 29, 197
BsuTUI ATCGAT 1 cut(s) 842
BtsIMutI CAGTG 1 cut(s) 907
Cac8I GCNNGC 4 cut(s) 40, 65, 330, 886
ClaI ATCGAT 1 cut(s) 842
Csp6I GTAC 3 cut(s) 280, 337, 465
CviAII CATG 6 cut(s) 39, 199, 415, 506, 851, 885
CviQI GTAC 3 cut(s) 280, 337, 465
DpnI GATC 2 cut(s) 266, 849
DpnII GATC 2 cut(s) 264, 847
Eam1104I CTCTTC 1 cut(s) 866
EarI CTCTTC 1 cut(s) 866
EciI GGCGGA 1 cut(s) 872
Eco130I CCWWGG 1 cut(s) 606
Eco147I AGGCCT 1 cut(s) 29
Eco57I CTGAAG 1 cut(s) 874
EcoRII CCWGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 606
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 6 cut(s) 42, 202, 418, 509, 854, 888
FatI CATG 6 cut(s) 38, 198, 414, 505, 850, 884
FblI GTMKAC 2 cut(s) 174, 246
FspBI CTAG 2 cut(s) 695, 922
GsuI CTGGAG 1 cut(s) 81
HaeIII GGCC 2 cut(s) 29, 197
HapII CCGG 2 cut(s) 242, 620
Hin1II CATG 6 cut(s) 42, 202, 418, 509, 854, 888
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 6 cut(s) 602, 627, 704, 779, 797, 815
HpaII CCGG 2 cut(s) 242, 620
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 3 cut(s) 63, 175, 247
Hpy188I TCNGA 3 cut(s) 680, 709, 811
Hpy188III TCNNGA 4 cut(s) 98, 154, 230, 719
Hpy8I GTNNAC 3 cut(s) 63, 175, 247
Hpy99I CGWCG 1 cut(s) 575
HpyCH4III ACNGT 3 cut(s) 279, 664, 768
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 364, 544, 547, 550
Hsp92II CATG 6 cut(s) 42, 202, 418, 509, 854, 888
Kzo9I GATC 2 cut(s) 264, 847
LmnI GCTCC 2 cut(s) 88, 304
LweI GCATC 1 cut(s) 565
MaeI CTAG 2 cut(s) 695, 922
MaeIII GTNAC 2 cut(s) 391, 517
MalI GATC 2 cut(s) 266, 849
MboI GATC 2 cut(s) 264, 847
MboII GAAGA 5 cut(s) 66, 169, 722, 824, 883
MhlI GDGCHC 3 cut(s) 65, 77, 830
MluCI AATT 4 cut(s) 272, 401, 407, 836
MlyI GAGTC 1 cut(s) 596
MnlI CCTC 8 cut(s) 17, 105, 187, 253, 406, 457, 513, 781
MroXI GAANNNNTTC 1 cut(s) 714
MseI TTAA 1 cut(s) 165
MspI CCGG 2 cut(s) 242, 620
MspR9I CCNGG 2 cut(s) 26, 621
MvaI CCWGG 1 cut(s) 26
MwoI GCNNNNNNNGC 5 cut(s) 35, 364, 544, 547, 550
NciI CCSGG 1 cut(s) 621
NdeII GATC 2 cut(s) 264, 847
NlaIII CATG 6 cut(s) 42, 202, 418, 509, 854, 888
NlaIV GGNNCC 2 cut(s) 16, 420
NmuCI GTSAC 1 cut(s) 391
NspI RCATGY 2 cut(s) 42, 888
PaeI GCATGC 2 cut(s) 42, 888
PceI AGGCCT 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 714
PfeI GAWTC 5 cut(s) 627, 704, 779, 797, 815
PflMI CCANNNNNTGG 1 cut(s) 289
PleI GAGTC 1 cut(s) 596
PpsI GAGTC 1 cut(s) 596
PshAI GACNNNNGTC 1 cut(s) 180
Psp6I CCWGG 1 cut(s) 24
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 2 cut(s) 16, 420
PstI CTGCAG 1 cut(s) 883
RsaI GTAC 3 cut(s) 281, 338, 466
RsaNI GTAC 3 cut(s) 280, 337, 465
SalI GTCGAC 1 cut(s) 173
SaqAI TTAA 1 cut(s) 165
Sau3AI GATC 2 cut(s) 264, 847
ScaI AGTACT 1 cut(s) 466
SchI GAGTC 1 cut(s) 596
ScrFI CCNGG 2 cut(s) 26, 621
SduI GDGCHC 3 cut(s) 65, 77, 830
SfaNI GCATC 1 cut(s) 565
SfcI CTRYAG 1 cut(s) 879
SmlI CTYRAG 2 cut(s) 8, 185
SmoI CTYRAG 2 cut(s) 8, 185
SphI GCATGC 2 cut(s) 42, 888
Sse9I AATT 4 cut(s) 272, 401, 407, 836
SseBI AGGCCT 1 cut(s) 29
SsiI CCGC 3 cut(s) 551, 854, 857
SspI AATATT 1 cut(s) 615
SspMI CTAG 2 cut(s) 695, 922
StuI AGGCCT 1 cut(s) 29
StyD4I CCNGG 2 cut(s) 24, 619
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 3 cut(s) 279, 664, 768
TaqI TCGA 5 cut(s) 174, 229, 565, 747, 842
TasI AATT 4 cut(s) 272, 401, 407, 836
TatI WGTACW 2 cut(s) 336, 464
TauI GCSGC 2 cut(s) 553, 857
TfiI GAWTC 5 cut(s) 627, 704, 779, 797, 815
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 1 cut(s) 907
TseFI GTSAC 1 cut(s) 391
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 2 cut(s) 215, 308
TspRI CASTG 1 cut(s) 907
Van91I CCANNNNNTGG 1 cut(s) 289
VneI GTGCAC 1 cut(s) 61
XceI RCATGY 2 cut(s) 42, 888
XmiI GTMKAC 2 cut(s) 174, 246
XmnI GAANNNNTTC 1 cut(s) 714
XspI CTAG 2 cut(s) 695, 922
ZrmI AGTACT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.