Rorug02G0539400

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005135
Physical Location & Seq
Reverse (-)
66902537 .. 66903271
735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0539400.1

Sequence Viewer

Length: 669 bp
ATGAACCCTCCCGCCGTACCCAGCCAACCCTCCTCCCCTCTCTTCCCGCTCTCGCCCAGTCCCCACCGCAAGGTCGCGATCGCCGTCGATTTAAGCGACGAGAGCGCCTACGCCGTCCGGTGGGCGGCCGAGCAGTACCTCCGACCGGGCGACGCCGTCGTCCTCCTCCACGTGCGCCCCACCAGCGTCCTCTACGGCGCCGACTGGGGCTGGGTCCCCGTCGACTACACCCTCCCCGTCGCCGGCGACAAGAAATCGGAGGACGACCTCGACGCGTTGACCACGGCCAAGACGACGGAGCTGGCGCGGCCGCTCGTGGAGGCCGGAATCGAGTTCAAAATCCACATAGTGAAGGATCGCGACATGAAGGAGAGGCTGTGCTTGGAGGTGGAGAGGCTCGGGCTCAGCGCCGTCATTATGGGCAGCCGAGGGTTCGGCGCCGCCGCCTCCAAGAGGAGCCTCAAGATGGCGAGGCTCGGAAGCGTGTCGGATTACTGTGTGCAGCACTGTGTGTGTCCCGTCGTGGTGGTGAGGTGTCCTGATGACGTGGAGCGGAGGTTTGGCGGCGATGGCGGTGGCAGTGAAGCGGCGGAGGACGTTCTGCTTCCGGTGCCGGAGGAGGACAATGAGGACGGTGAGCCTCGAGGTTTGGCTCTCTCTCTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

222

Amino Acids

23.95

Weight (kDa)

4.91

Isoelectric Point (pI)

56.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 23 - 178 3.1e-26 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AbsI CCTCGAGG 1 cut(s) 642
AccB1I GGYRCC 3 cut(s) 197, 437, 610
AccBSI CCGCTC 3 cut(s) 49, 313, 553
AccI GTMKAC 1 cut(s) 222
AccII CGCG 4 cut(s) 77, 275, 307, 360
AclWI GGATC 1 cut(s) 363
AcoI YGGCCR 3 cut(s) 126, 285, 308
AcvI CACGTG 1 cut(s) 172
AcyI GRCGYC 3 cut(s) 153, 198, 438
AdeI CACNNNGTG 2 cut(s) 349, 511
AfaI GTAC 2 cut(s) 18, 137
AfiI CCNNNNNNNGG 7 cut(s) 70, 120, 124, 145, 242, 453, 466
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 1 cut(s) 337
AjiI CACGTC 1 cut(s) 547
AluBI AGCT 1 cut(s) 301
AluI AGCT 1 cut(s) 301
AlwI GGATC 1 cut(s) 363
Ama87I CYCGRG 2 cut(s) 398, 642
AoxI GGCC 4 cut(s) 126, 285, 308, 321
ApeKI GCWGC 2 cut(s) 423, 502
AsiSI GCGATCGC 1 cut(s) 81
AspLEI GCGC 6 cut(s) 107, 177, 200, 307, 410, 440
AspS9I GGNCC 1 cut(s) 214
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 2 cut(s) 541, 647
AvaI CYCGRG 2 cut(s) 398, 642
AvaII GGWCC 1 cut(s) 214
BanI GGYRCC 3 cut(s) 197, 437, 610
BanII GRGCYC 1 cut(s) 405
BauI CACGAG 1 cut(s) 314
BbrPI CACGTG 1 cut(s) 172
BbvI GCAGC 2 cut(s) 435, 514
BccI CCATC 2 cut(s) 460, 563
BceAI ACGGC 6 cut(s) 68, 98, 140, 211, 300, 395
BcnI CCSGG 1 cut(s) 147
BfoI RGCGCY 4 cut(s) 108, 201, 411, 441
BisI GCNGC 9 cut(s) 126, 308, 311, 424, 441, 444, 503, 565, 588
BlpI GCTNAGC 1 cut(s) 404
BlsI GCNGC 9 cut(s) 127, 309, 312, 425, 442, 445, 504, 566, 589
Bme1390I CCNGG 1 cut(s) 147
Bme18I GGWCC 1 cut(s) 214
BmeT110I CYCGRG 2 cut(s) 398, 642
BmgBI CACGTC 1 cut(s) 547
BmgT120I GGNCC 1 cut(s) 214
BmiI GGNNCC 6 cut(s) 199, 215, 216, 439, 458, 612
BmrFI CCNGG 1 cut(s) 147
BmrI ACTGGG 2 cut(s) 51, 214
BmuI ACTGGG 2 cut(s) 51, 214
Bpu1102I GCTNAGC 1 cut(s) 404
BpuEI CTTGAG 1 cut(s) 446
BpuMI CCSGG 1 cut(s) 147
BsaAI YACGTR 1 cut(s) 172
BsaHI GRCGYC 3 cut(s) 153, 198, 438
BsaJI CCNNGG 2 cut(s) 282, 427
BsaWI WCCGGW 2 cut(s) 117, 607
BsaXI ACNNNNNCTCC 4 cut(s) 362, 392, 542, 572
Bsc4I CCNNNNNNNGG 7 cut(s) 70, 120, 124, 145, 242, 453, 466
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 2 cut(s) 57, 209
BseDI CCNNGG 2 cut(s) 282, 427
BseLI CCNNNNNNNGG 7 cut(s) 70, 120, 124, 145, 242, 453, 466
BseMII CTCAG 1 cut(s) 418
BseNI ACTGG 2 cut(s) 57, 209
BseRI GAGGAG 4 cut(s) 22, 155, 469, 632
BseX3I CGGCCG 2 cut(s) 126, 308
BseXI GCAGC 2 cut(s) 435, 514
BseYI CCCAGC 2 cut(s) 20, 210
BsgI GTGCAG 1 cut(s) 521
Bsh1236I CGCG 4 cut(s) 77, 275, 307, 360
Bsh1285I CGRYCG 4 cut(s) 81, 129, 146, 311
BshFI GGCC 4 cut(s) 128, 287, 310, 323
BshNI GGYRCC 3 cut(s) 197, 437, 610
BsiEI CGRYCG 4 cut(s) 81, 129, 146, 311
BsiHKCI CYCGRG 2 cut(s) 398, 642
BsiSI CCGG 6 cut(s) 118, 146, 243, 324, 608, 614
BslFI GGGAC 3 cut(s) 45, 200, 501
BslI CCNNNNNNNGG 7 cut(s) 70, 120, 124, 145, 242, 453, 466
BsmFI GGGAC 3 cut(s) 45, 200, 501
BsnI GGCC 4 cut(s) 128, 287, 310, 323
BsoBI CYCGRG 2 cut(s) 398, 642
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 2 cut(s) 78, 355
Bsp1720I GCTNAGC 1 cut(s) 404
Bsp68I TCGCGA 2 cut(s) 77, 360
BspANI GGCC 4 cut(s) 128, 287, 310, 323
BspCNI CTCAG 1 cut(s) 417
BspFNI CGCG 4 cut(s) 77, 275, 307, 360
BspLI GGNNCC 6 cut(s) 199, 215, 216, 439, 458, 612
BspPI GGATC 1 cut(s) 363
BspT107I GGYRCC 3 cut(s) 197, 437, 610
BsrBI CCGCTC 3 cut(s) 49, 313, 553
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 2 cut(s) 57, 209
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 2 cut(s) 282, 427
BssMI GATC 2 cut(s) 78, 355
BssNI GRCGYC 3 cut(s) 153, 198, 438
BssSI CACGAG 1 cut(s) 314
Bst2BI CACGAG 1 cut(s) 314
Bst4CI ACNGT 3 cut(s) 497, 509, 635
Bst6I CTCTTC 1 cut(s) 47
BstACI GRCGYC 3 cut(s) 153, 198, 438
BstBAI YACGTR 1 cut(s) 172
BstC8I GCNNGC 2 cut(s) 244, 303
BstDEI CTNAG 1 cut(s) 404
BstDSI CCRYGG 1 cut(s) 282
BstENI CCTNNNNNAGG 1 cut(s) 451
BstFNI CGCG 4 cut(s) 77, 275, 307, 360
BstH2I RGCGCY 4 cut(s) 108, 201, 411, 441
BstHHI GCGC 6 cut(s) 107, 177, 200, 307, 410, 440
BstKTI GATC 2 cut(s) 81, 358
BstMBI GATC 2 cut(s) 78, 355
BstMCI CGRYCG 4 cut(s) 81, 129, 146, 311
BstMWI GCNNNNNNNGC 5 cut(s) 102, 183, 307, 570, 610
BstSCI CCNGG 1 cut(s) 145
BstUI CGCG 4 cut(s) 77, 275, 307, 360
BstV1I GCAGC 2 cut(s) 435, 514
BstZI CGGCCG 2 cut(s) 126, 308
BsuRI GGCC 4 cut(s) 128, 287, 310, 323
BtgI CCRYGG 1 cut(s) 282
BtgZI GCGATG 1 cut(s) 582
BtrI CACGTC 1 cut(s) 547
BtsI GCAGTG 1 cut(s) 586
BtsIMutI CAGTG 2 cut(s) 505, 586
BtuMI TCGCGA 2 cut(s) 77, 360
Cac8I GCNNGC 2 cut(s) 244, 303
CciNI GCGGCCGC 1 cut(s) 308
CfoI GCGC 6 cut(s) 107, 177, 200, 307, 410, 440
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 1 cut(s) 214
CseI GACGC 3 cut(s) 161, 175, 281
Csp6I GTAC 2 cut(s) 17, 136
CviAII CATG 1 cut(s) 364
CviQI GTAC 2 cut(s) 17, 136
DdeI CTNAG 1 cut(s) 404
DinI GGCGCC 2 cut(s) 199, 439
DpnI GATC 2 cut(s) 80, 357
DpnII GATC 2 cut(s) 78, 355
DraIII CACNNNGTG 2 cut(s) 349, 511
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 3 cut(s) 126, 285, 308
EagI CGGCCG 2 cut(s) 126, 308
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
EciI GGCGGA 1 cut(s) 605
EclXI CGGCCG 2 cut(s) 126, 308
Eco24I GRGCYC 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 214
Eco52I CGGCCG 2 cut(s) 126, 308
Eco72I CACGTG 1 cut(s) 172
Eco88I CYCGRG 2 cut(s) 398, 642
EcoNI CCTNNNNNAGG 1 cut(s) 451
EcoO109I RGGNCCY 1 cut(s) 214
EcoT38I GRGCYC 1 cut(s) 405
EgeI GGCGCC 2 cut(s) 199, 439
EheI GGCGCC 2 cut(s) 199, 439
FaeI CATG 1 cut(s) 367
FaiI YATR 3 cut(s) 347, 365, 419
FaqI GGGAC 3 cut(s) 45, 200, 501
FatI CATG 1 cut(s) 363
FauI CCCGC 2 cut(s) 19, 54
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 9 cut(s) 126, 308, 311, 424, 441, 444, 503, 565, 588
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 9 cut(s) 126, 308, 311, 424, 441, 444, 503, 565, 588
GlaI GCGC 6 cut(s) 106, 176, 199, 306, 409, 439
GluI GCNGC 9 cut(s) 126, 308, 311, 424, 441, 444, 503, 565, 588
GsaI CCCAGC 2 cut(s) 24, 214
HaeII RGCGCY 4 cut(s) 108, 201, 411, 441
HaeIII GGCC 4 cut(s) 128, 287, 310, 323
HapII CCGG 6 cut(s) 118, 146, 243, 324, 608, 614
HgaI GACGC 3 cut(s) 161, 175, 281
HhaI GCGC 6 cut(s) 107, 177, 200, 307, 410, 440
Hin1I GRCGYC 3 cut(s) 153, 198, 438
Hin1II CATG 1 cut(s) 367
Hin6I GCGC 6 cut(s) 105, 175, 198, 305, 408, 438
HinP1I GCGC 6 cut(s) 105, 175, 198, 305, 408, 438
HincII GTYRAC 2 cut(s) 223, 279
HindII GTYRAC 2 cut(s) 223, 279
HinfI GANTC 1 cut(s) 327
HpaII CCGG 6 cut(s) 118, 146, 243, 324, 608, 614
HphI GGTGA 2 cut(s) 541, 647
Hpy166II GTNNAC 2 cut(s) 223, 279
Hpy188I TCNGA 5 cut(s) 143, 259, 479, 490, 663
Hpy188III TCNNGA 4 cut(s) 76, 359, 463, 539
Hpy8I GTNNAC 2 cut(s) 223, 279
Hpy99I CGWCG 9 cut(s) 89, 101, 155, 161, 224, 242, 275, 298, 524
HpyAV CCTTC 2 cut(s) 346, 361
HpyCH4III ACNGT 3 cut(s) 497, 509, 635
HpyCH4IV ACGT 3 cut(s) 171, 546, 597
HpyCH4V TGCA 1 cut(s) 502
HpyF10VI GCNNNNNNNGC 5 cut(s) 102, 183, 307, 570, 610
HpyF3I CTNAG 1 cut(s) 404
HpySE526I ACGT 3 cut(s) 171, 546, 597
Hsp92I GRCGYC 3 cut(s) 153, 198, 438
Hsp92II CATG 1 cut(s) 367
HspAI GCGC 6 cut(s) 105, 175, 198, 305, 408, 438
KasI GGCGCC 2 cut(s) 197, 437
KflI GGGWCCC 1 cut(s) 214
KroI GCCGGC 1 cut(s) 242
KroNI GCCGGC 1 cut(s) 244
Kzo9I GATC 2 cut(s) 78, 355
LmnI GCTCC 3 cut(s) 298, 456, 550
Lsp1109I GCAGC 2 cut(s) 435, 514
MaeII ACGT 3 cut(s) 171, 546, 597
MalI GATC 2 cut(s) 80, 357
MbiI CCGCTC 3 cut(s) 49, 313, 553
MboI GATC 2 cut(s) 78, 355
MboII GAAGA 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 405
MluI ACGCGT 1 cut(s) 273
Mly113I GGCGCC 2 cut(s) 198, 438
MmeI TCCRAC 2 cut(s) 166, 468
MreI CGCCGGCG 1 cut(s) 242
MroNI GCCGGC 1 cut(s) 242
MseI TTAA 2 cut(s) 92, 667
MspI CCGG 6 cut(s) 118, 146, 243, 324, 608, 614
MspR9I CCNGG 1 cut(s) 147
MvnI CGCG 4 cut(s) 77, 275, 307, 360
MwoI GCNNNNNNNGC 5 cut(s) 102, 183, 307, 570, 610
NaeI GCCGGC 1 cut(s) 244
NarI GGCGCC 2 cut(s) 198, 438
NciI CCSGG 1 cut(s) 147
NdeII GATC 2 cut(s) 78, 355
NgoMIV GCCGGC 1 cut(s) 242
NlaIII CATG 1 cut(s) 367
NlaIV GGNNCC 6 cut(s) 199, 215, 216, 439, 458, 612
NmeAIII GCCGAG 2 cut(s) 154, 452
NotI GCGGCCGC 1 cut(s) 308
NruI TCGCGA 2 cut(s) 77, 360
PaeR7I CTCGAG 1 cut(s) 642
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 93, 290
PdiI GCCGGC 1 cut(s) 244
PfeI GAWTC 1 cut(s) 327
PflFI GACNNNGTC 1 cut(s) 155
PkrI GCNGC 9 cut(s) 127, 309, 312, 425, 442, 445, 504, 566, 589
Ple19I CGATCG 1 cut(s) 81
PluTI GGCGCC 2 cut(s) 201, 441
PmaCI CACGTG 1 cut(s) 172
PmlI CACGTG 1 cut(s) 172
Ppu21I YACGTR 1 cut(s) 172
PpuMI RGGWCCY 1 cut(s) 214
Psp5II RGGWCCY 1 cut(s) 214
PspCI CACGTG 1 cut(s) 172
PspFI CCCAGC 2 cut(s) 20, 210
PspN4I GGNNCC 6 cut(s) 199, 215, 216, 439, 458, 612
PspPI GGNCC 1 cut(s) 214
PspPPI RGGWCCY 1 cut(s) 214
PspXI VCTCGAGB 1 cut(s) 642
PsyI GACNNNGTC 1 cut(s) 155
PvuI CGATCG 1 cut(s) 81
RgaI GCGATCGC 1 cut(s) 81
RruI TCGCGA 2 cut(s) 77, 360
RsaI GTAC 2 cut(s) 18, 137
RsaNI GTAC 2 cut(s) 17, 136
SalI GTCGAC 1 cut(s) 221
SaqAI TTAA 2 cut(s) 92, 667
SatI GCNGC 9 cut(s) 126, 308, 311, 424, 441, 444, 503, 565, 588
Sau3AI GATC 2 cut(s) 78, 355
Sau96I GGNCC 1 cut(s) 214
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 1 cut(s) 405
SfaAI GCGATCGC 1 cut(s) 81
SfoI GGCGCC 2 cut(s) 199, 439
Sfr274I CTCGAG 1 cut(s) 642
SgfI GCGATCGC 1 cut(s) 81
SgrAI CRCCGGYG 1 cut(s) 242
SinI GGWCC 1 cut(s) 214
SlaI CTCGAG 1 cut(s) 642
SmlI CTYRAG 2 cut(s) 461, 642
SmoI CTYRAG 2 cut(s) 461, 642
SspDI GGCGCC 2 cut(s) 197, 437
StyD4I CCNGG 1 cut(s) 145
TaaI ACNGT 3 cut(s) 497, 509, 635
TaiI ACGT 3 cut(s) 174, 549, 600
TaqI TCGA 5 cut(s) 87, 222, 270, 330, 643
TauI GCSGC 7 cut(s) 128, 310, 313, 443, 446, 567, 590
TfiI GAWTC 1 cut(s) 327
Tru1I TTAA 2 cut(s) 92, 667
Tru9I TTAA 2 cut(s) 92, 667
TscAI CASTG 2 cut(s) 512, 586
TseI GCWGC 2 cut(s) 423, 502
TspDTI ATGAA 2 cut(s) 17, 380
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 2 cut(s) 512, 586
Tth111I GACNNNGTC 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 214
XagI CCTNNNNNAGG 1 cut(s) 451
XhoI CTCGAG 1 cut(s) 642
XmiI GTMKAC 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.