MD06G1232300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002856
Physical Location & Seq
Reverse (-)
36318638 .. 36319501
864 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1232300.v1.1.491

Sequence Viewer

Length: 405 bp
ATGCTCAAAACCTCTGGCTTTGCTTATGCCTACGTTAACTTACACTCCATCATCCAGCTGCCCCCATATTCACATATATATCCAATTAGAGTCACGACCTTCACACACATAGGAAGCAAACTCATTAAGTCAGCGGAAGAGGAAGTTGCAATGGTGGTGTTAGGGCTAAACAAGCGCGTTTTTGCGTTCGTGGTTTTGATCTTCATCATCTCAATCGTCGTTGTTGATGGAGTTATAGGGGTCGACGAAAAAAGTGCAGATCTTATTCAAGACGAAGCCGCCAAGACCATTCATCCAGATTCATTCAAAACAAAACGAGAGAAAAATCTCATAACTGGACAAGATTTGGAGTTCCTGGTCATGGATTACACCCCAGCTAGAAGGAGGACCCCCATTCATAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

15.09

Weight (kDa)

8.0

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GLV1-2 PF21529 90 - 134 1.6e-06 Gloven 1/2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 243
AccII CGCG 1 cut(s) 177
AciI CCGC 2 cut(s) 134, 279
AfiI CCNNNNNNNGG 1 cut(s) 361
AgsI TTSAA 2 cut(s) 269, 307
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 2 cut(s) 58, 377
AluI AGCT 2 cut(s) 58, 377
ApeKI GCWGC 1 cut(s) 58
AspLEI GCGC 1 cut(s) 177
AspS9I GGNCC 1 cut(s) 387
AvaII GGWCC 1 cut(s) 387
BbvI GCAGC 1 cut(s) 45
BccI CCATC 2 cut(s) 56, 221
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BciT130I CCWGG 1 cut(s) 356
BfaI CTAG 1 cut(s) 378
BglII AGATCT 1 cut(s) 259
BisI GCNGC 2 cut(s) 59, 279
BlsI GCNGC 2 cut(s) 60, 280
Bme1390I CCNGG 1 cut(s) 356
Bme18I GGWCC 1 cut(s) 387
BmgT120I GGNCC 1 cut(s) 387
BmiI GGNNCC 1 cut(s) 389
BmrFI CCNGG 1 cut(s) 356
BsaBI GATNNNNATC 1 cut(s) 203
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 1 cut(s) 361
Bse1I ACTGG 1 cut(s) 340
Bse3DI GCAATG 1 cut(s) 156
Bse8I GATNNNNATC 1 cut(s) 203
BseBI CCWGG 1 cut(s) 356
BseGI GGATG 2 cut(s) 51, 292
BseJI GATNNNNATC 1 cut(s) 203
BseLI CCNNNNNNNGG 1 cut(s) 361
BseMI GCAATG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 340
BseXI GCAGC 1 cut(s) 45
BseYI CCCAGC 1 cut(s) 373
BsgI GTGCAG 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 177
BslI CCNNNNNNNGG 1 cut(s) 361
Bsp143I GATC 2 cut(s) 198, 259
BspACI CCGC 2 cut(s) 134, 279
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 156
BsrI ACTGG 1 cut(s) 340
BssMI GATC 2 cut(s) 198, 259
Bst2UI CCWGG 1 cut(s) 356
Bst6I CTCTTC 1 cut(s) 132
BstF5I GGATG 2 cut(s) 51, 292
BstFNI CGCG 1 cut(s) 177
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 2 cut(s) 201, 262
BstMBI GATC 2 cut(s) 198, 259
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 1 cut(s) 354
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
BtsCI GGATG 2 cut(s) 51, 292
CfoI GCGC 1 cut(s) 177
Cfr13I GGNCC 1 cut(s) 387
CviAII CATG 1 cut(s) 361
CviJI RGCY 5 cut(s) 18, 58, 166, 278, 377
CviKI_1 RGCY 5 cut(s) 18, 58, 166, 278, 377
DpnI GATC 2 cut(s) 200, 261
DpnII GATC 2 cut(s) 198, 259
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 387
EcoO109I RGGNCCY 1 cut(s) 387
EcoRII CCWGG 1 cut(s) 354
FaeI CATG 1 cut(s) 364
FatI CATG 1 cut(s) 360
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 2 cut(s) 59, 279
FokI GGATG 2 cut(s) 38, 279
Fsp4HI GCNGC 2 cut(s) 59, 279
FspBI CTAG 1 cut(s) 378
GlaI GCGC 1 cut(s) 176
GluI GCNGC 2 cut(s) 59, 279
GsaI CCCAGC 1 cut(s) 377
HhaI GCGC 1 cut(s) 177
Hin1II CATG 1 cut(s) 364
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HincII GTYRAC 2 cut(s) 37, 244
HindII GTYRAC 2 cut(s) 37, 244
HinfI GANTC 2 cut(s) 90, 299
HpaI GTTAAC 1 cut(s) 37
Hpy166II GTNNAC 2 cut(s) 37, 244
Hpy188III TCNNGA 3 cut(s) 94, 269, 296
Hpy8I GTNNAC 2 cut(s) 37, 244
Hpy99I CGWCG 2 cut(s) 221, 248
HpyAV CCTTC 2 cut(s) 109, 375
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 2 cut(s) 149, 257
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpySE526I ACGT 1 cut(s) 33
Hsp92II CATG 1 cut(s) 364
HspAI GCGC 1 cut(s) 175
KspAI GTTAAC 1 cut(s) 37
Kzo9I GATC 2 cut(s) 198, 259
LpnPI CCDG 6 cut(s) 68, 309, 321, 341, 368, 387
Lsp1109I GCAGC 1 cut(s) 45
MaeI CTAG 1 cut(s) 378
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 1 cut(s) 91
MalI GATC 2 cut(s) 200, 261
MboI GATC 2 cut(s) 198, 259
MboII GAAGA 2 cut(s) 149, 193
MflI RGATCY 1 cut(s) 259
MluCI AATT 1 cut(s) 84
MlyI GAGTC 1 cut(s) 99
MnlI CCTC 3 cut(s) 22, 133, 378
MseI TTAA 2 cut(s) 36, 126
MspA1I CMGCKG 2 cut(s) 58, 134
MspR9I CCNGG 1 cut(s) 356
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 1 cut(s) 172
NdeII GATC 2 cut(s) 198, 259
NlaIII CATG 1 cut(s) 364
NlaIV GGNNCC 1 cut(s) 389
NmuCI GTSAC 1 cut(s) 91
PfeI GAWTC 1 cut(s) 299
PkrI GCNGC 2 cut(s) 60, 280
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
PpuMI RGGWCCY 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 373
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 389
PspPI GGNCC 1 cut(s) 387
PspPPI RGGWCCY 1 cut(s) 387
PsuI RGATCY 1 cut(s) 259
PvuII CAGCTG 1 cut(s) 58
SalI GTCGAC 1 cut(s) 242
SaqAI TTAA 2 cut(s) 36, 126
SatI GCNGC 2 cut(s) 59, 279
Sau3AI GATC 2 cut(s) 198, 259
Sau96I GGNCC 1 cut(s) 387
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 1 cut(s) 356
SetI ASST 5 cut(s) 14, 36, 60, 101, 379
SinI GGWCC 1 cut(s) 387
Sse9I AATT 1 cut(s) 84
SsiI CCGC 2 cut(s) 134, 279
SspMI CTAG 1 cut(s) 378
StyD4I CCNGG 1 cut(s) 354
TaiI ACGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 243
TasI AATT 1 cut(s) 84
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 2 cut(s) 36, 126
Tru9I TTAA 2 cut(s) 36, 126
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 1 cut(s) 58
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 4 cut(s) 193, 281, 291, 386
VpaK11BI GGWCC 1 cut(s) 387
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.