Rorug02G0215000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002856
Physical Location & Seq
Forward (+)
20553695 .. 20557181
3487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0215000.1

Sequence Viewer

Length: 1266 bp
ATGAGTGTAGGCAAGCCGTCGTCGGCACCGCCGTCATCGCCGATGTTCTCCTTGCGAGGATCTCTCATCACTCTCGCCGTCTTCACCTTACTTTCCCTCGCCTACTTCTCCCTCAACTTCTCCGTCTCCTCCCCTCCCCCTCCCTCCGTCACCGCCACCGTCGACCGCCCCGTCGTCAGGCAGGAGGAGACTTCCGACTCCAATCCGTCACCCGAGGAGGAGGAGGAGGAGGAGTTTTCGGACATTTACCACTCGCCGGAGGTGTTCCGGTTGAACTACGCCGAAATGGAGCGGAAATTCAAGGTGTATATTTACCCCGACGGCGATCCGAACACATTTTACCAGACGCCGAGGAAGCTCACCGGGAAATATTCTAGTGAGGGATATTTCTTTCAGAATATTAGAAAGAGAAGGTTTCGGACCGAGGATCCGGATCAGGCTCAGCTCTTCTTCATCCCTATCTCCTGCCACAAGATGCGAGGCAAGGGTACTTCATATGAGAATATGACCATAATTGTTCAGAACTATGTGGAGAGCTTGATAGCCAAGTATCCGTACTGGAACAGGACTTTGGGTGCAGATCACTTCTTTGTGACTTGTCACGATGTTGGTGTGAGGGCAACTGAAGGACTTCCGCTTCTTGTAAAGAATTCTATTCGAGTTGTCTGCTCCCCTAGCTATGATGTTGGCTTCATTCCACATAAAGACGTCGCTCTCCCTCAAGTACTGCAACCATTTGCTCTTCCAGCTGGAGGAAACGATGTGGAGAACAGGACAACACTTGGTTTTTGGGCTGGTCATCGGAATTCAAAGATTAGAGTTATACTGGCACGAGAATGGGAGAATGATACAGAACTTTACATCTTGAACAACAGAATAAATAGAGCTGAAGGGAACTTGTTATATCAAAAGAAGTTTTATACTACCAAGTTCTGCATATGCCCAGGTGGTTCTCAGGTCAACAGTGCTCGCCCAATGGACTCAATCCATTATGGCTGTATTCCTGTAATATTATCAAATTACTATGACCTGCCGTTCAATGACATTCTTGACTGGCGAAAATTTTCTGTCATACTTACGGAGAAGGATGTGTATCAGCTTAAACAAATACTCAAGGCCATACCCTATTCAGAGTTTCTGACACTTCACAAAAACTTGGTGAAGGTCCAGAAGCACTTTCAGTGGAATTCACCGCCCATCAAATACGATGCATTCCACATGGTGATGTATGATCTTTGGTTGCGCCACCATGTTATTAAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

421

Amino Acids

48.64

Weight (kDa)

8.34

Isoelectric Point (pI)

54.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 97 - 373 3e-58 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 170
AatII GACGTC 1 cut(s) 711
Acc36I ACCTGC 1 cut(s) 1038
AccB1I GGYRCC 1 cut(s) 25
AccBSI CCGCTC 1 cut(s) 292
AccI GTMKAC 1 cut(s) 162
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 6 cut(s) 29, 153, 166, 292, 635, 1193
AclWI GGATC 5 cut(s) 67, 320, 422, 435, 441
AcsI RAATTY 5 cut(s) 296, 649, 805, 1061, 1186
AcuI CTGAAG 2 cut(s) 645, 909
AcyI GRCGYC 2 cut(s) 347, 708
AdeI CACNNNGTG 1 cut(s) 1222
AfaI GTAC 3 cut(s) 490, 557, 726
AfiI CCNNNNNNNGG 3 cut(s) 177, 256, 752
AgsI TTSAA 5 cut(s) 274, 301, 810, 868, 1039
AjnI CCWGG 1 cut(s) 943
AjuI GAANNNNNNNTTGG 2 cut(s) 554, 586
AluBI AGCT 7 cut(s) 358, 445, 537, 678, 749, 887, 1099
AluI AGCT 7 cut(s) 358, 445, 537, 678, 749, 887, 1099
Alw21I GWGCWC 1 cut(s) 970
Alw26I GTCTC 2 cut(s) 130, 182
AlwI GGATC 5 cut(s) 67, 320, 422, 435, 441
Ama87I CYCGRG 1 cut(s) 212
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 1 cut(s) 1116
ApoI RAATTY 5 cut(s) 296, 649, 805, 1061, 1186
Asp700I GAANNNNTTC 2 cut(s) 630, 1063
AspLEI GCGC 1 cut(s) 1245
AspS9I GGNCC 2 cut(s) 420, 1165
AsuC2I CCSGG 1 cut(s) 364
AsuHPI GGTGA 7 cut(s) 76, 142, 201, 352, 1171, 1182, 1234
AvaI CYCGRG 1 cut(s) 212
AvaII GGWCC 2 cut(s) 420, 1165
BamHI GGATCC 1 cut(s) 427
BanI GGYRCC 1 cut(s) 25
BauI CACGAG 1 cut(s) 831
BbsI GAAGAC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 970
BccI CCATC 1 cut(s) 1205
BceAI ACGGC 4 cut(s) 16, 62, 337, 1018
BcgI CGANNNNNNTGC 2 cut(s) 648, 682
BciT130I CCWGG 1 cut(s) 945
BciVI GTATCC 1 cut(s) 561
BcnI CCSGG 1 cut(s) 364
BcoDI GTCTC 2 cut(s) 130, 182
BfaI CTAG 2 cut(s) 375, 675
BfuAI ACCTGC 1 cut(s) 1038
BfuI GTATCC 1 cut(s) 561
BlpI GCTNAGC 1 cut(s) 441
BmcAI AGTACT 1 cut(s) 726
Bme1390I CCNGG 2 cut(s) 364, 945
Bme18I GGWCC 2 cut(s) 420, 1165
BmeT110I CYCGRG 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 420, 1165
BmiI GGNNCC 2 cut(s) 27, 429
BmrFI CCNGG 2 cut(s) 364, 945
BmsI GCATC 2 cut(s) 465, 1198
BpiI GAAGAC 1 cut(s) 73
BplI GAGNNNNNCTC 2 cut(s) 48, 80
BpmI CTGGAG 1 cut(s) 771
Bpu1102I GCTNAGC 1 cut(s) 441
BpuEI CTTGAG 2 cut(s) 705, 1097
BpuMI CCSGG 1 cut(s) 364
BsaBI GATNNNNATC 2 cut(s) 432, 1092
BsaHI GRCGYC 2 cut(s) 347, 708
BsaJI CCNNGG 4 cut(s) 213, 350, 423, 943
BsaWI WCCGGW 2 cut(s) 267, 430
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 256, 752
Bse1I ACTGG 3 cut(s) 563, 831, 1058
Bse8I GATNNNNATC 2 cut(s) 432, 1092
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 1 cut(s) 945
BseDI CCNNGG 4 cut(s) 213, 350, 423, 943
BseGI GGATG 2 cut(s) 453, 1093
BseJI GATNNNNATC 2 cut(s) 432, 1092
BseLI CCNNNNNNNGG 3 cut(s) 177, 256, 752
BseMII CTCAG 2 cut(s) 455, 968
BseNI ACTGG 3 cut(s) 563, 831, 1058
BseRI GAGGAG 8 cut(s) 118, 200, 230, 233, 236, 239, 242, 245
BsgI GTGCAG 1 cut(s) 597
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 1 cut(s) 1118
BshNI GGYRCC 1 cut(s) 25
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 970
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 4 cut(s) 257, 268, 363, 431
BslI CCNNNNNNNGG 3 cut(s) 177, 256, 752
BsmAI GTCTC 2 cut(s) 130, 182
BsmBI CGTCTC 1 cut(s) 130
BsmI GAATGC 1 cut(s) 1211
BsnI GGCC 1 cut(s) 1118
BsoBI CYCGRG 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 970
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 6 cut(s) 59, 325, 427, 433, 580, 1231
Bsp1720I GCTNAGC 1 cut(s) 441
BspACI CCGC 6 cut(s) 29, 153, 166, 292, 635, 1193
BspANI GGCC 1 cut(s) 1118
BspCNI CTCAG 2 cut(s) 454, 967
BspEI TCCGGA 1 cut(s) 430
BspLI GGNNCC 2 cut(s) 27, 429
BspMI ACCTGC 1 cut(s) 1038
BspPI GGATC 5 cut(s) 67, 320, 422, 435, 441
BspQI GCTCTTC 2 cut(s) 452, 747
BspT107I GGYRCC 1 cut(s) 25
BsrBI CCGCTC 1 cut(s) 292
BsrI ACTGG 3 cut(s) 563, 831, 1058
BssECI CCNNGG 4 cut(s) 213, 350, 423, 943
BssMI GATC 6 cut(s) 59, 325, 427, 433, 580, 1231
BssNI GRCGYC 2 cut(s) 347, 708
BssSI CACGAG 1 cut(s) 831
Bst2BI CACGAG 1 cut(s) 831
Bst2UI CCWGG 1 cut(s) 945
Bst4CI ACNGT 2 cut(s) 160, 965
Bst6I CTCTTC 2 cut(s) 452, 747
BstACI GRCGYC 2 cut(s) 347, 708
BstC8I GCNNGC 2 cut(s) 14, 970
BstDEI CTNAG 2 cut(s) 441, 954
BstF5I GGATG 2 cut(s) 453, 1093
BstHHI GCGC 1 cut(s) 1245
BstKTI GATC 6 cut(s) 62, 328, 430, 436, 583, 1234
BstMAI GTCTC 2 cut(s) 130, 182
BstMBI GATC 6 cut(s) 59, 325, 427, 433, 580, 1231
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 4 cut(s) 37, 355, 675, 746
BstNI CCWGG 1 cut(s) 945
BstSCI CCNGG 2 cut(s) 362, 943
BstV2I GAAGAC 1 cut(s) 73
BstX2I RGATCY 2 cut(s) 59, 427
BstYI RGATCY 2 cut(s) 59, 427
BsuI GTATCC 1 cut(s) 561
BsuRI GGCC 1 cut(s) 1118
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 2 cut(s) 453, 1093
BtsIMutI CAGTG 2 cut(s) 970, 1187
BveI ACCTGC 1 cut(s) 1038
Cac8I GCNNGC 2 cut(s) 14, 970
CfoI GCGC 1 cut(s) 1245
Cfr13I GGNCC 2 cut(s) 420, 1165
CpoI CGGWCCG 1 cut(s) 420
CseI GACGC 1 cut(s) 355
Csp6I GTAC 3 cut(s) 489, 556, 725
CspI CGGWCCG 1 cut(s) 420
CviAII CATG 2 cut(s) 1219, 1250
CviQI GTAC 3 cut(s) 489, 556, 725
DdeI CTNAG 2 cut(s) 441, 954
DpnI GATC 6 cut(s) 61, 327, 429, 435, 582, 1233
DpnII GATC 6 cut(s) 59, 325, 427, 433, 580, 1231
DraIII CACNNNGTG 1 cut(s) 1222
DrdI GACNNNNNNGTC 1 cut(s) 170
DseDI GACNNNNNNGTC 1 cut(s) 170
Eam1104I CTCTTC 2 cut(s) 452, 747
EarI CTCTTC 2 cut(s) 452, 747
Eco47I GGWCC 2 cut(s) 420, 1165
Eco57I CTGAAG 2 cut(s) 645, 909
Eco88I CYCGRG 1 cut(s) 212
EcoRI GAATTC 3 cut(s) 649, 805, 1186
EcoRII CCWGG 1 cut(s) 943
EcoT22I ATGCAT 1 cut(s) 1213
Esp3I CGTCTC 1 cut(s) 130
FaeI CATG 2 cut(s) 1222, 1253
FatI CATG 2 cut(s) 1218, 1249
FauNDI CATATG 2 cut(s) 496, 938
FblI GTMKAC 1 cut(s) 162
FokI GGATG 2 cut(s) 440, 1100
FspBI CTAG 2 cut(s) 375, 675
GlaI GCGC 1 cut(s) 1244
GsuI CTGGAG 1 cut(s) 771
HaeIII GGCC 1 cut(s) 1118
HapII CCGG 4 cut(s) 257, 268, 363, 431
HgaI GACGC 1 cut(s) 355
HhaI GCGC 1 cut(s) 1245
Hin1I GRCGYC 2 cut(s) 347, 708
Hin1II CATG 2 cut(s) 1222, 1253
Hin6I GCGC 1 cut(s) 1243
HinP1I GCGC 1 cut(s) 1243
HincII GTYRAC 2 cut(s) 163, 961
HindII GTYRAC 2 cut(s) 163, 961
HinfI GANTC 2 cut(s) 197, 980
HpaII CCGG 4 cut(s) 257, 268, 363, 431
HphI GGTGA 7 cut(s) 76, 142, 201, 352, 1171, 1182, 1234
Hpy166II GTNNAC 2 cut(s) 163, 961
Hpy188I TCNGA 9 cut(s) 196, 241, 330, 396, 420, 522, 804, 1132, 1140
Hpy188III TCNNGA 5 cut(s) 431, 602, 865, 1049, 1168
Hpy8I GTNNAC 2 cut(s) 163, 961
Hpy99I CGWCG 6 cut(s) 22, 25, 164, 176, 323, 713
HpyAV CCTTC 5 cut(s) 405, 620, 884, 1078, 1156
HpyCH4III ACNGT 2 cut(s) 160, 965
HpyCH4IV ACGT 1 cut(s) 708
HpyCH4V TGCA 4 cut(s) 578, 730, 936, 1211
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 355, 675, 746
HpyF3I CTNAG 2 cut(s) 441, 954
HpySE526I ACGT 1 cut(s) 708
Hsp92I GRCGYC 2 cut(s) 347, 708
Hsp92II CATG 2 cut(s) 1222, 1253
HspAI GCGC 1 cut(s) 1243
Kpn2I TCCGGA 1 cut(s) 430
Kzo9I GATC 6 cut(s) 59, 325, 427, 433, 580, 1231
LguI GCTCTTC 2 cut(s) 452, 747
LmnI GCTCC 2 cut(s) 289, 674
LweI GCATC 2 cut(s) 465, 1198
MaeI CTAG 2 cut(s) 375, 675
MaeII ACGT 1 cut(s) 708
MaeIII GTNAC 4 cut(s) 148, 207, 592, 599
MalI GATC 6 cut(s) 61, 327, 429, 435, 582, 1233
MbiI CCGCTC 1 cut(s) 292
MboI GATC 6 cut(s) 59, 325, 427, 433, 580, 1231
MboII GAAGA 4 cut(s) 73, 439, 442, 734
MflI RGATCY 2 cut(s) 59, 427
MhlI GDGCHC 1 cut(s) 970
MluCI AATT 7 cut(s) 296, 513, 649, 805, 1018, 1061, 1186
MlyI GAGTC 2 cut(s) 191, 974
MmeI TCCRAC 1 cut(s) 219
Mph1103I ATGCAT 1 cut(s) 1213
MroI TCCGGA 1 cut(s) 430
MroXI GAANNNNTTC 2 cut(s) 630, 1063
MseI TTAA 2 cut(s) 1101, 1257
MslI CAYNNNNRTG 2 cut(s) 835, 1223
MspA1I CMGCKG 1 cut(s) 749
MspI CCGG 4 cut(s) 257, 268, 363, 431
MspR9I CCNGG 2 cut(s) 364, 945
Mva1269I GAATGC 1 cut(s) 1211
MvaI CCWGG 1 cut(s) 945
MwoI GCNNNNNNNGC 4 cut(s) 37, 355, 675, 746
NciI CCSGG 1 cut(s) 364
NdeI CATATG 2 cut(s) 496, 938
NdeII GATC 6 cut(s) 59, 325, 427, 433, 580, 1231
NlaIII CATG 2 cut(s) 1222, 1253
NlaIV GGNNCC 2 cut(s) 27, 429
NmeAIII GCCGAG 1 cut(s) 375
NmuCI GTSAC 4 cut(s) 148, 207, 592, 599
NsiI ATGCAT 1 cut(s) 1213
PciSI GCTCTTC 2 cut(s) 452, 747
PcsI WCGNNNNNNNCGW 2 cut(s) 29, 168
PctI GAATGC 1 cut(s) 1211
PdmI GAANNNNTTC 2 cut(s) 630, 1063
PleI GAGTC 2 cut(s) 191, 974
PpsI GAGTC 2 cut(s) 191, 974
Psp6I CCWGG 1 cut(s) 943
PspGI CCWGG 1 cut(s) 943
PspN4I GGNNCC 2 cut(s) 27, 429
PspPI GGNCC 2 cut(s) 420, 1165
PsrI GAACNNNNNNTAC 2 cut(s) 323, 355
PsuI RGATCY 2 cut(s) 59, 427
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 3 cut(s) 490, 557, 726
RsaNI GTAC 3 cut(s) 489, 556, 725
RseI CAYNNNNRTG 2 cut(s) 835, 1223
Rsr2I CGGWCCG 1 cut(s) 420
RsrII CGGWCCG 1 cut(s) 420
SalI GTCGAC 1 cut(s) 161
SapI GCTCTTC 2 cut(s) 452, 747
SaqAI TTAA 2 cut(s) 1101, 1257
Sau3AI GATC 6 cut(s) 59, 325, 427, 433, 580, 1231
Sau96I GGNCC 2 cut(s) 420, 1165
ScaI AGTACT 1 cut(s) 726
SchI GAGTC 2 cut(s) 191, 974
ScrFI CCNGG 2 cut(s) 364, 945
SduI GDGCHC 1 cut(s) 970
SfaNI GCATC 2 cut(s) 465, 1198
SinI GGWCC 2 cut(s) 420, 1165
SmiMI CAYNNNNRTG 2 cut(s) 835, 1223
SmlI CTYRAG 2 cut(s) 720, 1112
SmoI CTYRAG 2 cut(s) 720, 1112
Sse9I AATT 7 cut(s) 296, 513, 649, 805, 1018, 1061, 1186
SsiI CCGC 6 cut(s) 29, 153, 166, 292, 635, 1193
SspI AATATT 3 cut(s) 371, 400, 1011
SspMI CTAG 2 cut(s) 375, 675
StyD4I CCNGG 2 cut(s) 362, 943
TaaI ACNGT 2 cut(s) 160, 965
TaiI ACGT 1 cut(s) 711
TaqI TCGA 2 cut(s) 162, 658
TaqII GACCGA 1 cut(s) 437
TasI AATT 7 cut(s) 296, 513, 649, 805, 1018, 1061, 1186
TatI WGTACW 1 cut(s) 724
Tru1I TTAA 2 cut(s) 1101, 1257
Tru9I TTAA 2 cut(s) 1101, 1257
TscAI CASTG 2 cut(s) 970, 1187
TseFI GTSAC 4 cut(s) 148, 207, 592, 599
Tsp45I GTSAC 4 cut(s) 148, 207, 592, 599
TspDTI ATGAA 3 cut(s) 442, 483, 682
TspGWI ACGGA 5 cut(s) 112, 136, 195, 543, 1094
TspRI CASTG 2 cut(s) 970, 1187
VpaK11BI GGWCC 2 cut(s) 420, 1165
XapI RAATTY 5 cut(s) 296, 649, 805, 1061, 1186
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 2 cut(s) 630, 1063
XspI CTAG 2 cut(s) 375, 675
ZraI GACGTC 1 cut(s) 709
ZrmI AGTACT 1 cut(s) 726
Zsp2I ATGCAT 1 cut(s) 1213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.