Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0003601

Physical Location & Seq

Genomic Coordinates Reverse (-)

31336858 .. 31344211

Gene Structure 7354 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1243100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2703 bp

ATGCCGGATGAGTATTTGAAGTATTTGCTTTCTCTCAACGAAAGGCAGCGTGAGGCAGCTTGCAGCTATATTTCAGTACCTTTGATGATTGTTGCCGGCCCTGGAAGTGGAAAGACTTCAACGATAGTTGGGCGCGTTCTAATGCTGCTCAATGAGGGCATTAGCACGTCGAACATTCTAGCAATGACTTTCACTACAGCAGCAGCTTCCGAGATGAGAGATCGAATTGGATCTGTGACTGGGAAAGCAACGGCAAAACTACTAACAATCAGTACATTTCATTCATTTTCATTGCAACTTTGCCGATCACATGCTGAGAAACTCAAGCGCAGACCAGAATTTTTGATATATGGACATGGGCAACAGAGAAGAGCAATCATTGAAGCTGTGCGGTTATCAGAAGATGAAAAGAGTAAAACGAATCTTAATGTTGCTATAGATGGGGAAGAGTCTGACGGCGTAACATCTCCACATCATTTTAAGGATGCATCAAAGAAATGGCTGAAGTTTGTGACACAGGCAAAAGCTTCTGGAAAGACTCCCGCTGAATGCCGAAGAATGGGTGATGTGACAGGAGGTGAAATTCTTAGGAATTACAATGATATCTTGAAATCGTGCAACGCTTTGGACTACCACGACTTAATTAGCTGCTCTGTGAAGCTGCTAAGTGATTTTCCTGAAGTGTTGAAGGAGTGCCAGGAGTCATGGAAAGCTATTGTAATAGACGAGTTTCAAGACACAAGTGCTATGCAATATAGTCTTTTGCGGATTCTCGCCTCTCATAACCACATAACCATTGTTGGTGATGATGATCAGTCCATTTTCAGTTTCAATGGGGCGGACATTTCTGGATTTGATTCTTTTCGTAAAGATTTTTCAAATTACAAAGAGATTAGGCTTAATAAAAACTTTAGGTCCACACGCTATATTGTGGAAGCTGCATCTTCTGTTATAAAAAACAATAAGAAGCGATGCCAACTAAAGAATGTTGACACGGACAATTCTTCTGGGTCTAAGGTAATTATCAAGGAAAGTCACAATGAGGATGCACAATGCGCCTTTGTTGTCGACAAGATCCTGGAGACTGCATCGAAACGGTCAGAAGCAGGGTGCTCCTATGGAAATATAGCAATCCTTTACCGGAGGCAGGTCAAGCTTACTTGCCTTAGTGCTAATGTGAATGAGGTATCAGGAAAAGTCTTCCAGACAGCCTTGCGTGAAAGGAAAATACCATTTAATGTTCATGGAGTGGCAGTTTATAGGAAGAAGGTAGTTAGAGCCATTGTTGCTATGATTAAAACAACTTTGCCTGGTTGTGATGATGGATCATATAGGCAAGTCTTCAAGGCTCTACTTGCTTTTGAGAAAGAGGAAAAGAAGAAGGTGATCGATTATATTGACAAAATTTCAACTCACAGAAAATGCAGCTTCATATCAGCTGCTTGTGACATCTTTAGTGCAAAAATTTCTGGTACCTTGAAGAGGACTCAGCTTACCCAAGGACGCAAGGTGCTGTTAACACTAGAGATGATTTCAAAACTTGTTCTCAGGGAACAATCGATTTCAGCTGTCATAACCTCAGTTGCGAATATGGTACCACAGTACCGTCTTGAGCAACGTGCAGTCGTTGATGTAGATGGAGGAAAACTGCTGAATGAAGACAATGACGTGAGATCTGTTCTTCAGTACCTACTAGACGACAGAGAAGTCGTAGCAGAAGAAAAAGGCTGCGTTAATTTACTCAAAGCTTTCATCGACTACATATCTGAGCAGGAGAGTGAAAATTTTCACTCCCGAAGACATGACAACCAAAGTTCTGTCACTTTGACTACTATTCATCAGTCAAAAGGTTTAGAGTGGGATATAGTTTTCATAATTAAGGTAAATGAATCTGAGATCCCGTTGTTGCATGAATTCAATGGGGTTGCAAAAGAAAATGGGACCTCCATTGAGGAGGAGAGACGCCTATTATATGTTGCAATGACTCGTGCTCGAAAAAAGCTTTTCATTCTTTATGTTACGATGGATTCCAGTTGGCAGATGCTTCAACCTTCGCGATTTCTGAAAGAAATTCCAAATCATCTACGAGAAGTCCAGGCTGACGTAAGTACAGAAGGCTTGCAATCAAAGCATCATGACACTTCAAAAGAAACTACTCCGTCAACAGATGATCTGAAACCAAATCTCCTACCTTCTGAAGTTATTACAGTGCAAAATGATCTCCATCATAGTAAATTTGACGTTGCCTCTGCAGAATTGATGGAATTTGGGGAGGAAAACCATGGAAATGGCTTCTTAAAAAGATTCAGTGTGGATGAGAGATCAATTTTCTCGCATGTATTTAATGACTGGGCTAAGAAGGAAGCATTTCAAGATCCTAAGAGATTACTTGACAAGATTGGTTTTGTAATAGATGAACGCATACGAGCCAAGAACTACAAACACAAGGACGGTTTGCGCTCACTCAAATCTTGCTTGAAAGATGAAGATGCATTTCATTATGCCCAATATGTTTTGAGATGGCAGAAAATACCTGCAGATCAACGCGCTCATTTGATGAGGGAAAAACAGGTTAGGTGTAAGACGGTTCCCTTTGTCACACTTTGGTTGTTTTCGGTAGCAAACTATTGCTTTGTGTTTGAGAACGACAGTATCGATTGCGGTTGGCAAATATGCCGACTTATAGAACAAACACCATTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003678 GO:0003824 GO:0004386 GO:0006139 GO:0006259 GO:0006310 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0032392 GO:0032508 GO:0034641 GO:0036292 GO:0043138 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0046483 GO:0051276 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UvrD-helicase UvrD_C

Protein Analysis

901

Amino Acids

102.21

Weight (kDa)

8.54

Isoelectric Point (pI)

39.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UvrD-helicase	PF00580	12 - 292	1.8e-68	UvrD/REP helicase N-terminal domain
AAA_11	PF13086	12 - 272	7.8e-06	AAA domain
AAA_19	PF13245	16 - 277	2.1e-22	AAA domain
UvrD_C	PF13361	298 - 673	3.9e-40	UvrD-like helicase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003601)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25120
fragaria_vesca	FvH4_7g26710 FvH4_7g26710 FvH4_7g26710 FvH4_7g26710
malus_domestica	MD07G1241600.v1.1 MD07G1241700.v1.1 MD07G1243100.v1.1 MD07G1243600.v1.1 MD07G1244100.v1.1 MD07G1244200.v1.1 MD07G1244300.v1.1 MD07G1244800.v1.1 MD07G1245200.v1.1 MD07G1245700.v1.1 MD07G1245800.v1.1
prunus_persica	Prupe.2G270600_v2.0.a1 Prupe.2G270600_v2.0.a1
pyrus_communis	pycom07g21810 pycom07g21980
rosa_chinensis	RchiOBHm_Chr1g0373301
rosa_laevigata	RLG00000001620 RLG00000006922 RLG00000035899
rosa_multiflora	Rmu_ssc0000435.1_g000004
rosa_roxburghii	Rroxscaffold_4G00284280
rosa_rugosa	Rorug01G0376900
rosa_samantha	Rh1AG385900 Rh1BG350100 Rh1CG363200 Rh1DG380900 Rh3CG316100 Rh5CG053300 Rh5DG386500
rosa_wichuraiana	Rw1G034900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	953, 2701
AasI	GACNNNNNNGTC	1 cut(s)	1706
Acc36I	ACCTGC	2 cut(s)	1138, 2542
Acc65I	GGTACC	2 cut(s)	1472, 1594
AccB1I	GGYRCC	2 cut(s)	1472, 1594
AccI	GTMKAC	1 cut(s)	1068
AccII	CGCG	3 cut(s)	135, 2056, 2547
AciI	CCGC	5 cut(s)	391, 543, 766, 839, 2661
AclWI	GGATC	5 cut(s)	238, 1069, 1333, 1891, 2369
AcsI	RAATTY	9 cut(s)	338, 582, 1404, 1464, 1783, 1913, 2070, 2234, 2264
AcuI	CTGAAG	4 cut(s)	524, 699, 1667, 2217
AcyI	GRCGYC	1 cut(s)	1963
AfaI	GTAC	7 cut(s)	78, 274, 1474, 1596, 1604, 1688, 2110
AfiI	CCNNNNNNNGG	4 cut(s)	107, 559, 1147, 1482
AjiI	CACGTC	2 cut(s)	168, 1669
AjnI	CCWGG	5 cut(s)	100, 696, 1077, 1309, 2094
Alw21I	GWGCWC	2 cut(s)	1115, 1993
Alw26I	GTCTC	2 cut(s)	1076, 1954
AlwI	GGATC	5 cut(s)	238, 1069, 1333, 1891, 2369
AoxI	GGCC	1 cut(s)	97
ApoI	RAATTY	9 cut(s)	338, 582, 1404, 1464, 1783, 1913, 2070, 2234, 2264
Asp700I	GAANNNNTTC	3 cut(s)	115, 1785, 2367
Asp718I	GGTACC	2 cut(s)	1472, 1594
AspLEI	GCGC	5 cut(s)	135, 330, 1058, 2460, 2549
AspS9I	GGNCC	3 cut(s)	98, 915, 1941
AsuHPI	GGTGA	4 cut(s)	575, 590, 815, 1396
AvaII	GGWCC	2 cut(s)	915, 1941
BaeI	ACNNNNGTAYC	6 cut(s)	1586, 1586, 1619, 1619, 2635, 2668
BanI	GGYRCC	2 cut(s)	1472, 1594
BauI	CACGAG	1 cut(s)	1986
BbsI	GAAGAC	4 cut(s)	1192, 1333, 1665, 1804
Bbv12I	GWGCWC	2 cut(s)	1115, 1993
BccI	CCATC	7 cut(s)	434, 1316, 1631, 2017, 2232, 2254, 2514
BceAI	ACGGC	2 cut(s)	267, 472
BciT130I	CCWGG	5 cut(s)	102, 698, 1079, 1311, 2096
BclI	TGATCA	1 cut(s)	811
BcoDI	GTCTC	2 cut(s)	1076, 1954
BfaI	CTAG	3 cut(s)	179, 1523, 1694
BfmI	CTRYAG	4 cut(s)	195, 435, 2250, 2535
BfuAI	ACCTGC	2 cut(s)	1138, 2542
BglII	AGATCT	1 cut(s)	1673
Bme1390I	CCNGG	5 cut(s)	102, 698, 1079, 1311, 2096
Bme18I	GGWCC	2 cut(s)	915, 1941
BmgBI	CACGTC	2 cut(s)	168, 1669
BmgT120I	GGNCC	3 cut(s)	98, 915, 1941
BmiI	GGNNCC	4 cut(s)	1474, 1596, 1942, 2589
BmrFI	CCNGG	5 cut(s)	102, 698, 1079, 1311, 2096
BmrI	ACTGGG	2 cut(s)	249, 2359
BmsI	GCATC	9 cut(s)	475, 497, 950, 962, 1036, 1097, 2031, 2140, 2479
BmuI	ACTGGG	2 cut(s)	249, 2359
BpiI	GAAGAC	4 cut(s)	1192, 1333, 1665, 1804
BpmI	CTGGAG	1 cut(s)	1100
BpuEI	CTTGAG	2 cut(s)	308, 1631
Bsa29I	ATCGAT	3 cut(s)	1389, 1559, 2655
BsaBI	GATNNNNATC	2 cut(s)	810, 2223
BsaHI	GRCGYC	1 cut(s)	1963
BsaJI	CCNNGG	3 cut(s)	100, 1498, 2281
BsaWI	WCCGGW	1 cut(s)	1140
BsaXI	ACNNNNNCTCC	4 cut(s)	1239, 1269, 2169, 2199
Bsc4I	CCNNNNNNNGG	4 cut(s)	107, 559, 1147, 1482
Bse118I	RCCGGY	1 cut(s)	95
Bse1I	ACTGG	3 cut(s)	244, 2031, 2354
Bse3DI	GCAATG	3 cut(s)	189, 290, 1986
Bse8I	GATNNNNATC	2 cut(s)	810, 2223
BseBI	CCWGG	5 cut(s)	102, 698, 1079, 1311, 2096
BseCI	ATCGAT	3 cut(s)	1389, 1559, 2655
BseDI	CCNNGG	3 cut(s)	100, 1498, 2281
BseGI	GGATG	4 cut(s)	13, 490, 1051, 2320
BseJI	GATNNNNATC	2 cut(s)	810, 2223
BseLI	CCNNNNNNNGG	4 cut(s)	107, 559, 1147, 1482
BseMI	GCAATG	3 cut(s)	189, 290, 1986
BseMII	CTCAG	6 cut(s)	306, 1502, 1561, 1593, 1758, 1884
BseNI	ACTGG	3 cut(s)	244, 2031, 2354
BseRI	GAGGAG	2 cut(s)	1967, 1970
BsgI	GTGCAG	1 cut(s)	1641
Bsh1236I	CGCG	3 cut(s)	135, 2056, 2547
BshFI	GGCC	1 cut(s)	99
BshNI	GGYRCC	2 cut(s)	1472, 1594
BshVI	ATCGAT	3 cut(s)	1389, 1559, 2655
BsiHKAI	GWGCWC	2 cut(s)	1115, 1993
BsiSI	CCGG	3 cut(s)	5, 96, 1141
BslFI	GGGAC	1 cut(s)	1954
BslI	CCNNNNNNNGG	4 cut(s)	107, 559, 1147, 1482
BsmAI	GTCTC	2 cut(s)	1076, 1954
BsmBI	CGTCTC	1 cut(s)	1954
BsmFI	GGGAC	1 cut(s)	1954
BsmI	GAATGC	1 cut(s)	554
BsnI	GGCC	1 cut(s)	99
Bsp1286I	GDGCHC	2 cut(s)	1115, 1993
Bsp19I	CCATGG	1 cut(s)	2281
Bsp68I	TCGCGA	1 cut(s)	2056
BspACI	CCGC	5 cut(s)	391, 543, 766, 839, 2661
BspANI	GGCC	1 cut(s)	99
BspCNI	CTCAG	6 cut(s)	307, 1501, 1560, 1592, 1759, 1885
BspDI	ATCGAT	3 cut(s)	1389, 1559, 2655
BspFNI	CGCG	3 cut(s)	135, 2056, 2547
BspHI	TCATGA	1 cut(s)	2134
BspLI	GGNNCC	4 cut(s)	1474, 1596, 1942, 2589
BspMAI	CTGCAG	2 cut(s)	2254, 2539
BspMI	ACCTGC	2 cut(s)	1138, 2542
BspPI	GGATC	5 cut(s)	238, 1069, 1333, 1891, 2369
BspQI	GCTCTTC	1 cut(s)	364
BspT107I	GGYRCC	2 cut(s)	1472, 1594
BsrDI	GCAATG	3 cut(s)	189, 290, 1986
BsrFI	RCCGGY	1 cut(s)	95
BsrI	ACTGG	3 cut(s)	244, 2031, 2354
BssAI	RCCGGY	1 cut(s)	95
BssECI	CCNNGG	3 cut(s)	100, 1498, 2281
BssNI	GRCGYC	1 cut(s)	1963
BssSI	CACGAG	1 cut(s)	1986
BssT1I	CCWWGG	2 cut(s)	1498, 2281
Bst2BI	CACGAG	1 cut(s)	1986
Bst2UI	CCWGG	5 cut(s)	102, 698, 1079, 1311, 2096
Bst4CI	ACNGT	7 cut(s)	1098, 1602, 1607, 2209, 2453, 2587, 2651
Bst6I	CTCTTC	3 cut(s)	364, 441, 1475
BstACI	GRCGYC	1 cut(s)	1963
BstC8I	GCNNGC	3 cut(s)	61, 97, 2120
BstDSI	CCRYGG	1 cut(s)	2281
BstENI	CCTNNNNNAGG	1 cut(s)	1480
BstF5I	GGATG	4 cut(s)	13, 490, 1051, 2320
BstFNI	CGCG	3 cut(s)	135, 2056, 2547
BstHHI	GCGC	5 cut(s)	135, 330, 1058, 2460, 2549
BstMAI	GTCTC	2 cut(s)	1076, 1954
BstMWI	GCNNNNNNNGC	5 cut(s)	1055, 1153, 1286, 1355, 2128
BstNI	CCWGG	5 cut(s)	102, 698, 1079, 1311, 2096
BstNSI	RCATGY	2 cut(s)	314, 2339
BstSCI	CCNGG	5 cut(s)	100, 696, 1077, 1309, 2094
BstSFI	CTRYAG	4 cut(s)	195, 435, 2250, 2535
BstUI	CGCG	3 cut(s)	135, 2056, 2547
BstV2I	GAAGAC	4 cut(s)	1192, 1333, 1665, 1804
BstX2I	RGATCY	5 cut(s)	230, 1074, 1673, 1896, 2374
BstXI	CCANNNNNNTGG	1 cut(s)	2288
BstYI	RGATCY	5 cut(s)	230, 1074, 1673, 1896, 2374
Bsu15I	ATCGAT	3 cut(s)	1389, 1559, 2655
BsuRI	GGCC	1 cut(s)	99
BsuTUI	ATCGAT	3 cut(s)	1389, 1559, 2655
BtgI	CCRYGG	1 cut(s)	2281
BtgZI	GCGATG	1 cut(s)	985
BtrI	CACGTC	2 cut(s)	168, 1669
BtsCI	GGATG	4 cut(s)	13, 490, 1051, 2320
BtsIMutI	CAGTG	2 cut(s)	2214, 2314
BtuMI	TCGCGA	1 cut(s)	2056
BveI	ACCTGC	2 cut(s)	1138, 2542
Cac8I	GCNNGC	3 cut(s)	61, 97, 2120
CciI	TCATGA	1 cut(s)	2134
CfoI	GCGC	5 cut(s)	135, 330, 1058, 2460, 2549
Cfr10I	RCCGGY	1 cut(s)	95
Cfr13I	GGNCC	3 cut(s)	98, 915, 1941
ClaI	ATCGAT	3 cut(s)	1389, 1559, 2655
CseI	GACGC	2 cut(s)	1512, 1971
Csp6I	GTAC	7 cut(s)	77, 273, 1473, 1595, 1603, 1687, 2109
CviAII	CATG	9 cut(s)	311, 356, 705, 1244, 1802, 1910, 2135, 2282, 2336
CviQI	GTAC	7 cut(s)	77, 273, 1473, 1595, 1603, 1687, 2109
DrdI	GACNNNNNNGTC	1 cut(s)	1706
DseDI	GACNNNNNNGTC	1 cut(s)	1706
Eam1104I	CTCTTC	3 cut(s)	364, 441, 1475
EarI	CTCTTC	3 cut(s)	364, 441, 1475
EciI	GGCGGA	1 cut(s)	854
Eco130I	CCWWGG	2 cut(s)	1498, 2281
Eco32I	GATATC	1 cut(s)	604
Eco47I	GGWCC	2 cut(s)	915, 1941
Eco57I	CTGAAG	4 cut(s)	524, 699, 1667, 2217
EcoNI	CCTNNNNNAGG	1 cut(s)	1480
EcoO109I	RGGNCCY	1 cut(s)	1941
EcoRI	GAATTC	1 cut(s)	1913
EcoRII	CCWGG	5 cut(s)	100, 696, 1077, 1309, 2094
EcoRV	GATATC	1 cut(s)	604
EcoT14I	CCWWGG	2 cut(s)	1498, 2281
EcoT22I	ATGCAT	2 cut(s)	490, 2494
ErhI	CCWWGG	2 cut(s)	1498, 2281
Esp3I	CGTCTC	1 cut(s)	1954
FaeI	CATG	9 cut(s)	314, 359, 708, 1247, 1805, 1913, 2138, 2285, 2339
FaqI	GGGAC	1 cut(s)	1954
FatI	CATG	9 cut(s)	310, 355, 704, 1243, 1801, 1909, 2134, 2281, 2335
FauI	CCCGC	1 cut(s)	550
FbaI	TGATCA	1 cut(s)	811
FblI	GTMKAC	1 cut(s)	1068
FokI	GGATG	4 cut(s)	20, 497, 1058, 2327
FspBI	CTAG	3 cut(s)	179, 1523, 1694
GlaI	GCGC	5 cut(s)	134, 329, 1057, 2459, 2548
GsuI	CTGGAG	1 cut(s)	1100
HaeIII	GGCC	1 cut(s)	99
HapII	CCGG	3 cut(s)	5, 96, 1141
HgaI	GACGC	2 cut(s)	1512, 1971
HhaI	GCGC	5 cut(s)	135, 330, 1058, 2460, 2549
Hin1I	GRCGYC	1 cut(s)	1963
Hin1II	CATG	9 cut(s)	314, 359, 708, 1247, 1805, 1913, 2138, 2285, 2339
Hin6I	GCGC	5 cut(s)	133, 328, 1056, 2458, 2547
HinP1I	GCGC	5 cut(s)	133, 328, 1056, 2458, 2547
HincII	GTYRAC	4 cut(s)	991, 1069, 1518, 2163
HindII	GTYRAC	4 cut(s)	991, 1069, 1518, 2163
HindIII	AAGCTT	4 cut(s)	525, 1154, 1746, 2000
HpaI	GTTAAC	1 cut(s)	1518
HpaII	CCGG	3 cut(s)	5, 96, 1141
HphI	GGTGA	4 cut(s)	575, 590, 815, 1396
Hpy166II	GTNNAC	5 cut(s)	918, 991, 1069, 1518, 2163
Hpy188I	TCNGA	9 cut(s)	211, 400, 454, 1102, 1768, 1894, 2064, 2175, 2197
Hpy8I	GTNNAC	5 cut(s)	918, 991, 1069, 1518, 2163
Hpy99I	CGWCG	1 cut(s)	172
HpyAV	CCTTC	7 cut(s)	682, 1261, 1375, 2061, 2108, 2202, 2353
HpyCH4III	ACNGT	7 cut(s)	1098, 1602, 1607, 2209, 2453, 2587, 2651
HpyCH4IV	ACGT	5 cut(s)	167, 1618, 1668, 2103, 2241
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1055, 1153, 1286, 1355, 2128
HpySE526I	ACGT	5 cut(s)	167, 1618, 1668, 2103, 2241
Hsp92I	GRCGYC	1 cut(s)	1963
Hsp92II	CATG	9 cut(s)	314, 359, 708, 1247, 1805, 1913, 2138, 2285, 2339
HspAI	GCGC	5 cut(s)	133, 328, 1056, 2458, 2547
KpnI	GGTACC	2 cut(s)	1476, 1598
KroI	GCCGGC	1 cut(s)	95
KroNI	GCCGGC	1 cut(s)	97
Ksp22I	TGATCA	1 cut(s)	811
KspAI	GTTAAC	1 cut(s)	1518
LguI	GCTCTTC	1 cut(s)	364
LmnI	GCTCC	1 cut(s)	1118
LweI	GCATC	9 cut(s)	475, 497, 950, 962, 1036, 1097, 2031, 2140, 2479
MaeI	CTAG	3 cut(s)	179, 1523, 1694
MaeII	ACGT	5 cut(s)	167, 1618, 1668, 2103, 2241
MaeIII	GTNAC	9 cut(s)	235, 460, 511, 568, 1034, 1445, 1819, 2017, 2596
MflI	RGATCY	5 cut(s)	230, 1074, 1673, 1896, 2374
MhlI	GDGCHC	2 cut(s)	1115, 1993
MlyI	GAGTC	5 cut(s)	458, 532, 710, 1480, 1978
Mph1103I	ATGCAT	2 cut(s)	490, 2494
MroNI	GCCGGC	1 cut(s)	95
MroXI	GAANNNNTTC	3 cut(s)	115, 1785, 2367
MslI	CAYNNNNRTG	1 cut(s)	2286
MspA1I	CMGCKG	3 cut(s)	545, 1439, 1568
MspI	CCGG	3 cut(s)	5, 96, 1141
MspR9I	CCNGG	5 cut(s)	102, 698, 1079, 1311, 2096
Mva1269I	GAATGC	1 cut(s)	554
MvaI	CCWGG	5 cut(s)	102, 698, 1079, 1311, 2096
MvnI	CGCG	3 cut(s)	135, 2056, 2547
MwoI	GCNNNNNNNGC	5 cut(s)	1055, 1153, 1286, 1355, 2128
NaeI	GCCGGC	1 cut(s)	97
NcoI	CCATGG	1 cut(s)	2281
NgoMIV	GCCGGC	1 cut(s)	95
NlaIII	CATG	9 cut(s)	314, 359, 708, 1247, 1805, 1913, 2138, 2285, 2339
NlaIV	GGNNCC	4 cut(s)	1474, 1596, 1942, 2589
NmuCI	GTSAC	7 cut(s)	235, 511, 568, 1034, 1445, 1819, 2596
NruI	TCGCGA	1 cut(s)	2056
NsiI	ATGCAT	2 cut(s)	490, 2494
NspI	RCATGY	2 cut(s)	314, 2339
PagI	TCATGA	1 cut(s)	2134
PciSI	GCTCTTC	1 cut(s)	364
PcsI	WCGNNNNNNNCGW	1 cut(s)	2652
PctI	GAATGC	1 cut(s)	554
PdiI	GCCGGC	1 cut(s)	97
PdmI	GAANNNNTTC	3 cut(s)	115, 1785, 2367
PfeI	GAWTC	6 cut(s)	421, 769, 857, 1889, 2027, 2304
PfoI	TCCNGGA	1 cut(s)	1077
PleI	GAGTC	5 cut(s)	457, 532, 709, 1480, 1978
PpsI	GAGTC	5 cut(s)	457, 532, 709, 1480, 1978
PpuMI	RGGWCCY	1 cut(s)	1941
PsiI	TTATAA	2 cut(s)	953, 2701
Psp5II	RGGWCCY	1 cut(s)	1941
Psp6I	CCWGG	5 cut(s)	100, 696, 1077, 1309, 2094
PspGI	CCWGG	5 cut(s)	100, 696, 1077, 1309, 2094
PspN4I	GGNNCC	4 cut(s)	1474, 1596, 1942, 2589
PspPI	GGNCC	3 cut(s)	98, 915, 1941
PspPPI	RGGWCCY	1 cut(s)	1941
PstI	CTGCAG	2 cut(s)	2254, 2539
PsuI	RGATCY	5 cut(s)	230, 1074, 1673, 1896, 2374
PvuII	CAGCTG	2 cut(s)	1439, 1568
RruI	TCGCGA	1 cut(s)	2056
RsaI	GTAC	7 cut(s)	78, 274, 1474, 1596, 1604, 1688, 2110
RsaNI	GTAC	7 cut(s)	77, 273, 1473, 1595, 1603, 1687, 2109
RseI	CAYNNNNRTG	1 cut(s)	2286
SalI	GTCGAC	1 cut(s)	1067
SapI	GCTCTTC	1 cut(s)	364
Sau96I	GGNCC	3 cut(s)	98, 915, 1941
SchI	GAGTC	5 cut(s)	458, 532, 710, 1480, 1978
ScrFI	CCNGG	5 cut(s)	102, 698, 1079, 1311, 2096
SduI	GDGCHC	2 cut(s)	1115, 1993
SfaNI	GCATC	9 cut(s)	475, 497, 950, 962, 1036, 1097, 2031, 2140, 2479
SfcI	CTRYAG	4 cut(s)	195, 435, 2250, 2535
SinI	GGWCC	2 cut(s)	915, 1941
SmiMI	CAYNNNNRTG	1 cut(s)	2286
SmlI	CTYRAG	2 cut(s)	323, 1610
SmoI	CTYRAG	2 cut(s)	323, 1610
SsiI	CCGC	5 cut(s)	391, 543, 766, 839, 2661
SspMI	CTAG	3 cut(s)	179, 1523, 1694
StyD4I	CCNGG	5 cut(s)	100, 696, 1077, 1309, 2094
StyI	CCWWGG	2 cut(s)	1498, 2281
TaaI	ACNGT	7 cut(s)	1098, 1602, 1607, 2209, 2453, 2587, 2651
TaiI	ACGT	5 cut(s)	170, 1621, 1671, 2106, 2244
TaqI	TCGA	9 cut(s)	170, 223, 1068, 1091, 1389, 1559, 1755, 1993, 2655
TatI	WGTACW	2 cut(s)	272, 2108
TfiI	GAWTC	6 cut(s)	421, 769, 857, 1889, 2027, 2304
TscAI	CASTG	2 cut(s)	2214, 2314
TseFI	GTSAC	7 cut(s)	235, 511, 568, 1034, 1445, 1819, 2596
Tsp45I	GTSAC	7 cut(s)	235, 511, 568, 1034, 1445, 1819, 2596
TspGWI	ACGGA	2 cut(s)	1010, 2148
TspRI	CASTG	2 cut(s)	2214, 2314
VpaK11BI	GGWCC	2 cut(s)	915, 1941
XagI	CCTNNNNNAGG	1 cut(s)	1480
XapI	RAATTY	9 cut(s)	338, 582, 1404, 1464, 1783, 1913, 2070, 2234, 2264
XceI	RCATGY	2 cut(s)	314, 2339
XmiI	GTMKAC	1 cut(s)	1068
XmnI	GAANNNNTTC	3 cut(s)	115, 1785, 2367
XspI	CTAG	3 cut(s)	179, 1523, 1694
Zsp2I	ATGCAT	2 cut(s)	490, 2494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.