MD07G1243600.v1.1

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003601
Physical Location & Seq
Reverse (-)
31383411 .. 31392344
8934 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1243600.v1.1.491

Sequence Viewer

Length: 2898 bp
ATGTCCAAGGAAAACGTTCAGGAATACGGCGGAGGGCTCACGGCGGAACAAAGAAACCGCATCTCTCGGAATTTCAGAGCCGCCAAGGCCCTCATTGATCGCAAACGCCCCCGCAACGACGCCGTTTCACCTGATCATCACCTCCCTCATACCAAAATCTGTGGCGGAAATGGAATCCAAACGCCAACTCAGCTCAATAGAGTTCAAAGAACACCGCCTCCGATTTCCGCAAGCCGGTACAATTCAACGCGTGGTGAATATGGCAGTGCTTCGTGTTCCGGAAGCTTAAGCGTTAGCGGGACCGAATCAAGCGTGTCAAAATCGGATTCTTTCACAACTCCAATCAGACAACCGCAGTTTCTGACTTCATTTGATTCTTTCTCTGCATCTGGAATTCTTAACGATGATGATTTCGATGAGAGTATTTTGCAGGAAATTGACGCTTTAATCGAGCAGAAACCGAGTGTGGACATTCAGCGTGATGATATTGGGAGTTGTGGTAGTGACGTTAGTGCCGCATCAGCGCCGCCTTTGAGTAGTGAAAACCAAACAGATTCTGTTAACCATGGATGGCCTAACATGCCGGATGAGTATTTGAAGTATTTGCTTTCTCTCAACGAAAGGCAGCGCGAGGCAGCTTGCAGCGATATTTCAGTACCTTTGATGATTGTTGCCGGCCCTGGAAGTGGAAAGACTTCAACGATAGTTGGGCGCGTTCTAATGCTGCTCAATGAGGGCATTAGCACGTCGAACATTCTAGCAATGACTTTCACTACAGCAGCAGCTTCCGAGATGAGAGATCGAATTGGATCTGTGACTGGGAAAGCAACGGCAAAACTACTAACAATCAGTACATTTCATTCATTTTCATTGCAACTTTGCCGATCACATGCTGAGAAACTCGAGCGCAGACCAGAATTTTTGATATATGGACATGGGCAACAGAGAAGAGCAATCATTGAAGCTGTGCGGTTATCAGAAGATGAAAAGAGTAAAACGAATCTTAATGTTGCTATAGATGGGGAAGAGTCTAACGGCGTAACATCTCCACATCATTTTAAGGATGCATCAAAGAAATGGCTGAAGTTTGTGACACAGGCAAAAGCTTCTGGAAAGACTCCCGCTGAATGCCGAAGAATGGGTGATGTGACAGGAGGTGAAATTCTTAGGAATTACAATGATATCTTGAAATCGTGCAACGCTTTGGACTACCACGACTTAATTAGCTGCTCTGTGAAGCTGCTAAGTGATTTTCCTGAAGTGTTGAAGGAGTGCCAGGAGTCATGGAAAGCTATTGTAATAGACGAGTTTCAAGACACAAGTGCTATGCAATATAGTCTTTTGCGGATTCTCGCCTCTCATAACCACATAACCATTGTTGGCGATGATGATCAGTCCATTTTCAGTTTCAATGGGGCGGACATTTCTGGATTTGATTATTTTCGTAAAGATTTTTCAAATTACAAAAAGATTAGGCTTAATAAAAACTTTCGGTCCACACGCTATATTGTGGAAGCTGCATCTTCTGTTATAAAAAACAATAAGAAGCGATGCCAACTAAAGAATGTTGACACGGACAATTCTTCTGGGTCTAAGGTAATTATCAAGGAAAGTCACAATGAGGATGCACAATGTGCCTTTGTTGTAGTTAGAGCCATTGTTGCTATGATTAAAACAACTTTGCCTGGTTGTGATGATGGATCATATAGGCAAGTCTTCAAGGCTCTACTTGCTTTTGAGAAAGAGGAAAGAAGAAGAAAATGCAGCTTCATATCAGCTGCTTGTGACATCTTTAGTGCAAAAATTTCTGGTACCTTGAAGAGGACTCAGCTTACCCAAGGACGCAAGGTGCTGTTAACACTAGAGATGATTTCAAAACTTGTTCTCAGGGAACAATCGATTTCAGCTGTCATAACCTCAGTTGCGAATATGGTACCACAGTACCGTCTTGAGCAACGTGCAGTCGTTGATGTAGATGGAGGAAAACTGCTGAATGAAGACAATGACATGAGATCTGTTCTTCAGTACCTACTAGACGACGTATCTGATTTCTTATCAACTCATTTTGTTGTGGGAAAAGGGGAGAGAGAAGTCGTAGCAGAAGAAAAAGGCTGCGTTAATTTACTCAAAGCTTTCATCGACTACATATCTGAGCGGGAGAGTGAAAATTTTCACTCCCGAAGACATGACAACCAAAGTTCTGTCACTTTGACTACTATTCATCAGTCAAAAGGTTTAGAGTGGGATATGGTTTTCATAATTAAGATGCTTCAACCTTCGCGATTTCTGAAAGAAATTCCAAATCATCTACGAGAAGTCCAGGCTGACGTAAGTACAGAAGGCTTGCAATCAAAGCATCATGACACTTCAAAAGAAACTACTCCGTCAACAGATGATCTCAAACCAAATCTCCTACCTTCTGAAGTTGTTATGGTGCAAAATGATCTCCATCATAGTAAATTTGACGTTGCCTCTGCAGAATTGATGGAATTTGGGGAGGAAAACCATGGAAATGGCTTCTTAAAAAGATTCAGTGTGGATGAGAGATCGATTATCTCGCATGTATTTAATGACTGGGCTAAGAAGCAAGCATTTCAAGATCCTAAGAGATTACTTGACAAGGACGGTTTGCGCTCACTGAAATCTTGCTTGAAAGATGAAGATGCATTTCATTATGCCCAATATGTTTTGAGATGGCAGAAAATACCTGCAGATCAACGCGCTCATTTGATGAGGGAAAAACAGGAGCATTTCCAGAAGTTAAGGATCGAGAACTCAATGGGTTCATCCGAACCAACACCGAAACAGATTTCTTATCTGCAAAGTTTGGGCTGCACCGTGGCTCCTACGTCGTGCCTCCATGCGTCTCGTTTGATCGAGCAGTACAAGTCATTATGA

Protein Analysis

966

Amino Acids

108.04

Weight (kDa)

6.86

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UvrD-helicase PF00580 205 - 485 5.6e-68 UvrD/REP helicase N-terminal domain
AAA_19 PF13245 211 - 470 3.4e-22 AAA domain
UvrD_C PF13361 491 - 550 9.4e-07 UvrD-like helicase C-terminal domain
UvrD_C PF13361 702 - 756 1.6e-07 UvrD-like helicase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1532
Acc36I ACCTGC 1 cut(s) 2716
Acc65I GGTACC 2 cut(s) 1811, 1933
AccB1I GGYRCC 2 cut(s) 1811, 1933
AccBSI CCGCTC 1 cut(s) 2155
AccII CGCG 5 cut(s) 250, 630, 714, 2281, 2721
AccIII TCCGGA 1 cut(s) 278
AclI AACGTT 1 cut(s) 15
AclWI GGATC 4 cut(s) 817, 1708, 2594, 2774
AcsI RAATTY 9 cut(s) 70, 393, 917, 1161, 1803, 2167, 2295, 2459, 2489
AcuI CTGAAG 4 cut(s) 1103, 1278, 2006, 2442
AcyI GRCGYC 1 cut(s) 120
AdeI CACNNNGTG 1 cut(s) 1634
AfaI GTAC 9 cut(s) 239, 657, 853, 1813, 1935, 1943, 2027, 2335, 2885
AfiI CCNNNNNNNGG 4 cut(s) 234, 686, 1138, 1821
AflII CTTAAG 1 cut(s) 286
AflIII ACRYGT 1 cut(s) 248
AjiI CACGTC 1 cut(s) 747
AjnI CCWGG 4 cut(s) 679, 1275, 1684, 2319
AloI GAACNNNNNNTCC 2 cut(s) 202, 234
Alw26I GTCTC 1 cut(s) 2871
AlwI GGATC 4 cut(s) 817, 1708, 2594, 2774
AlwNI CAGNNNCTG 2 cut(s) 361, 557
Ama87I CYCGRG 1 cut(s) 902
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 3 cut(s) 87, 572, 676
ApoI RAATTY 9 cut(s) 70, 393, 917, 1161, 1803, 2167, 2295, 2459, 2489
Asp700I GAANNNNTTC 3 cut(s) 15, 694, 2169
Asp718I GGTACC 2 cut(s) 1811, 1933
AspLEI GCGC 6 cut(s) 526, 630, 714, 909, 2634, 2723
AspS9I GGNCC 4 cut(s) 88, 300, 677, 1494
AsuHPI GGTGA 5 cut(s) 120, 131, 266, 1154, 1169
AvaI CYCGRG 1 cut(s) 902
AvaII GGWCC 2 cut(s) 300, 1494
BaeI ACNNNNGTAYC 4 cut(s) 1925, 1925, 1958, 1958
BanI GGYRCC 2 cut(s) 1811, 1933
BanII GRGCYC 1 cut(s) 39
BbsI GAAGAC 3 cut(s) 1708, 2004, 2188
BccI CCATC 7 cut(s) 564, 1013, 1691, 1970, 2457, 2479, 2688
BceAI ACGGC 5 cut(s) 43, 57, 107, 846, 1051
BciT130I CCWGG 4 cut(s) 681, 1277, 1686, 2321
BclI TGATCA 2 cut(s) 133, 1390
BcoDI GTCTC 1 cut(s) 2871
BfaI CTAG 3 cut(s) 758, 1862, 2033
BfmI CTRYAG 4 cut(s) 774, 1014, 2475, 2709
BfoI RGCGCY 1 cut(s) 527
BfrI CTTAAG 1 cut(s) 286
BfuAI ACCTGC 1 cut(s) 2716
BglI GCCNNNNNGGC 1 cut(s) 86
BglII AGATCT 1 cut(s) 2012
Bme1390I CCNGG 4 cut(s) 681, 1277, 1686, 2321
Bme18I GGWCC 2 cut(s) 300, 1494
BmeT110I CYCGRG 1 cut(s) 902
BmgBI CACGTC 1 cut(s) 747
BmgT120I GGNCC 4 cut(s) 88, 300, 677, 1494
BmiI GGNNCC 4 cut(s) 301, 1813, 1935, 2844
BmrFI CCNGG 4 cut(s) 681, 1277, 1686, 2321
BmrI ACTGGG 2 cut(s) 828, 2584
BmuI ACTGGG 2 cut(s) 828, 2584
BpiI GAAGAC 3 cut(s) 1708, 2004, 2188
BpuEI CTTGAG 1 cut(s) 1970
Bsa29I ATCGAT 2 cut(s) 1898, 2549
BsaBI GATNNNNATC 2 cut(s) 1389, 2448
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 7 cut(s) 6, 84, 565, 679, 1837, 2506, 2838
BsaWI WCCGGW 1 cut(s) 278
BsaXI ACNNNNNCTCC 8 cut(s) 202, 232, 484, 514, 2394, 2424, 2827, 2857
Bsc4I CCNNNNNNNGG 4 cut(s) 234, 686, 1138, 1821
Bse118I RCCGGY 2 cut(s) 234, 674
Bse1I ACTGG 2 cut(s) 823, 2579
Bse3DI GCAATG 2 cut(s) 768, 869
Bse8I GATNNNNATC 2 cut(s) 1389, 2448
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 4 cut(s) 681, 1277, 1686, 2321
BseCI ATCGAT 2 cut(s) 1898, 2549
BseDI CCNNGG 7 cut(s) 6, 84, 565, 679, 1837, 2506, 2838
BseGI GGATG 6 cut(s) 575, 592, 1069, 1630, 2545, 2786
BseJI GATNNNNATC 2 cut(s) 1389, 2448
BseLI CCNNNNNNNGG 4 cut(s) 234, 686, 1138, 1821
BseMI GCAATG 2 cut(s) 768, 869
BseMII CTCAG 6 cut(s) 203, 885, 1841, 1900, 1932, 2142
BseNI ACTGG 2 cut(s) 823, 2579
BsgI GTGCAG 2 cut(s) 1980, 2818
Bsh1236I CGCG 5 cut(s) 250, 630, 714, 2281, 2721
BshFI GGCC 3 cut(s) 89, 574, 678
BshNI GGYRCC 2 cut(s) 1811, 1933
BshVI ATCGAT 2 cut(s) 1898, 2549
BsiHKCI CYCGRG 1 cut(s) 902
BsiSI CCGG 4 cut(s) 235, 279, 584, 675
BslFI GGGAC 1 cut(s) 313
BslI CCNNNNNNNGG 4 cut(s) 234, 686, 1138, 1821
BsmAI GTCTC 1 cut(s) 2871
BsmBI CGTCTC 1 cut(s) 2871
BsmFI GGGAC 1 cut(s) 313
BsmI GAATGC 1 cut(s) 1133
BsnI GGCC 3 cut(s) 89, 574, 678
BsoBI CYCGRG 1 cut(s) 902
Bsp1286I GDGCHC 1 cut(s) 39
Bsp13I TCCGGA 1 cut(s) 278
Bsp19I CCATGG 2 cut(s) 565, 2506
Bsp68I TCGCGA 1 cut(s) 2281
BspANI GGCC 3 cut(s) 89, 574, 678
BspCNI CTCAG 6 cut(s) 202, 886, 1840, 1899, 1931, 2143
BspDI ATCGAT 2 cut(s) 1898, 2549
BspEI TCCGGA 1 cut(s) 278
BspFNI CGCG 5 cut(s) 250, 630, 714, 2281, 2721
BspHI TCATGA 1 cut(s) 2359
BspLI GGNNCC 4 cut(s) 301, 1813, 1935, 2844
BspMAI CTGCAG 2 cut(s) 2479, 2713
BspMI ACCTGC 1 cut(s) 2716
BspPI GGATC 4 cut(s) 817, 1708, 2594, 2774
BspQI GCTCTTC 1 cut(s) 943
BspT107I GGYRCC 2 cut(s) 1811, 1933
BspTI CTTAAG 1 cut(s) 286
BsrBI CCGCTC 1 cut(s) 2155
BsrDI GCAATG 2 cut(s) 768, 869
BsrFI RCCGGY 2 cut(s) 234, 674
BsrI ACTGG 2 cut(s) 823, 2579
BssAI RCCGGY 2 cut(s) 234, 674
BssECI CCNNGG 7 cut(s) 6, 84, 565, 679, 1837, 2506, 2838
BssNI GRCGYC 1 cut(s) 120
BssT1I CCWWGG 5 cut(s) 6, 84, 565, 1837, 2506
Bst2UI CCWGG 4 cut(s) 681, 1277, 1686, 2321
Bst4CI ACNGT 4 cut(s) 1941, 1946, 2627, 2839
Bst6I CTCTTC 3 cut(s) 943, 1020, 1814
BstACI GRCGYC 1 cut(s) 120
BstAFI CTTAAG 1 cut(s) 286
BstAPI GCANNNNNTGC 1 cut(s) 1634
BstC8I GCNNGC 5 cut(s) 232, 640, 676, 2345, 2589
BstDSI CCRYGG 3 cut(s) 565, 2506, 2838
BstENI CCTNNNNNAGG 1 cut(s) 1819
BstF5I GGATG 6 cut(s) 575, 592, 1069, 1630, 2545, 2786
BstFNI CGCG 5 cut(s) 250, 630, 714, 2281, 2721
BstH2I RGCGCY 1 cut(s) 527
BstHHI GCGC 6 cut(s) 526, 630, 714, 909, 2634, 2723
BstMAI GTCTC 1 cut(s) 2871
BstMWI GCNNNNNNNGC 8 cut(s) 86, 190, 521, 580, 1634, 1661, 1730, 2353
BstNI CCWGG 4 cut(s) 681, 1277, 1686, 2321
BstNSI RCATGY 3 cut(s) 583, 893, 2564
BstSCI CCNGG 4 cut(s) 679, 1275, 1684, 2319
BstSFI CTRYAG 4 cut(s) 774, 1014, 2475, 2709
BstUI CGCG 5 cut(s) 250, 630, 714, 2281, 2721
BstV2I GAAGAC 3 cut(s) 1708, 2004, 2188
BstX2I RGATCY 3 cut(s) 809, 2012, 2599
BstXI CCANNNNNNTGG 1 cut(s) 2513
BstYI RGATCY 3 cut(s) 809, 2012, 2599
Bsu15I ATCGAT 2 cut(s) 1898, 2549
BsuRI GGCC 3 cut(s) 89, 574, 678
BsuTUI ATCGAT 2 cut(s) 1898, 2549
BtgI CCRYGG 3 cut(s) 565, 2506, 2838
BtgZI GCGATG 2 cut(s) 1398, 1564
BtrI CACGTC 1 cut(s) 747
BtsCI GGATG 6 cut(s) 575, 592, 1069, 1630, 2545, 2786
BtsI GCAGTG 1 cut(s) 271
BtsIMutI CAGTG 3 cut(s) 271, 2539, 2636
BtuMI TCGCGA 1 cut(s) 2281
BveI ACCTGC 1 cut(s) 2716
Cac8I GCNNGC 5 cut(s) 232, 640, 676, 2345, 2589
CaiI CAGNNNCTG 2 cut(s) 361, 557
CciI TCATGA 1 cut(s) 2359
CfoI GCGC 6 cut(s) 526, 630, 714, 909, 2634, 2723
Cfr10I RCCGGY 2 cut(s) 234, 674
Cfr13I GGNCC 4 cut(s) 88, 300, 677, 1494
ClaI ATCGAT 2 cut(s) 1898, 2549
CseI GACGC 4 cut(s) 128, 449, 1851, 2853
Csp6I GTAC 9 cut(s) 238, 656, 852, 1812, 1934, 1942, 2026, 2334, 2884
CspCI CAANNNNNGTGG 2 cut(s) 142, 177
CviQI GTAC 9 cut(s) 238, 656, 852, 1812, 1934, 1942, 2026, 2334, 2884
DraIII CACNNNGTG 1 cut(s) 1634
Eam1104I CTCTTC 3 cut(s) 943, 1020, 1814
EarI CTCTTC 3 cut(s) 943, 1020, 1814
EciI GGCGGA 4 cut(s) 45, 59, 180, 1433
Eco130I CCWWGG 5 cut(s) 6, 84, 565, 1837, 2506
Eco24I GRGCYC 1 cut(s) 39
Eco32I GATATC 1 cut(s) 1183
Eco47I GGWCC 2 cut(s) 300, 1494
Eco57I CTGAAG 4 cut(s) 1103, 1278, 2006, 2442
Eco88I CYCGRG 1 cut(s) 902
EcoNI CCTNNNNNAGG 1 cut(s) 1819
EcoO109I RGGNCCY 1 cut(s) 88
EcoRI GAATTC 1 cut(s) 393
EcoRII CCWGG 4 cut(s) 679, 1275, 1684, 2319
EcoRV GATATC 1 cut(s) 1183
EcoT14I CCWWGG 5 cut(s) 6, 84, 565, 1837, 2506
EcoT22I ATGCAT 2 cut(s) 1069, 2668
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 5 cut(s) 6, 84, 565, 1837, 2506
Esp3I CGTCTC 1 cut(s) 2871
FaqI GGGAC 1 cut(s) 313
FauI CCCGC 4 cut(s) 119, 290, 1129, 2148
FbaI TGATCA 2 cut(s) 133, 1390
FokI GGATG 6 cut(s) 582, 599, 1076, 1637, 2552, 2773
FriOI GRGCYC 1 cut(s) 39
FspBI CTAG 3 cut(s) 758, 1862, 2033
GlaI GCGC 6 cut(s) 525, 629, 713, 908, 2633, 2722
HaeII RGCGCY 1 cut(s) 527
HaeIII GGCC 3 cut(s) 89, 574, 678
HapII CCGG 4 cut(s) 235, 279, 584, 675
HgaI GACGC 4 cut(s) 128, 449, 1851, 2853
HhaI GCGC 6 cut(s) 526, 630, 714, 909, 2634, 2723
Hin1I GRCGYC 1 cut(s) 120
Hin6I GCGC 6 cut(s) 524, 628, 712, 907, 2632, 2721
HinP1I GCGC 6 cut(s) 524, 628, 712, 907, 2632, 2721
HincII GTYRAC 4 cut(s) 562, 1570, 1857, 2388
HindII GTYRAC 4 cut(s) 562, 1570, 1857, 2388
HindIII AAGCTT 3 cut(s) 283, 1104, 2130
HpaI GTTAAC 2 cut(s) 562, 1857
HpaII CCGG 4 cut(s) 235, 279, 584, 675
HphI GGTGA 5 cut(s) 120, 131, 266, 1154, 1169
Hpy166II GTNNAC 6 cut(s) 469, 562, 1497, 1570, 1857, 2388
Hpy8I GTNNAC 6 cut(s) 469, 562, 1497, 1570, 1857, 2388
Hpy99I CGWCG 4 cut(s) 122, 751, 2042, 2854
HpyAV CCTTC 4 cut(s) 1261, 2286, 2333, 2427
HpyCH4III ACNGT 4 cut(s) 1941, 1946, 2627, 2839
HpyCH4IV ACGT 8 cut(s) 15, 507, 746, 1957, 2040, 2328, 2466, 2849
HpyF10VI GCNNNNNNNGC 8 cut(s) 86, 190, 521, 580, 1634, 1661, 1730, 2353
HpySE526I ACGT 8 cut(s) 15, 507, 746, 1957, 2040, 2328, 2466, 2849
Hsp92I GRCGYC 1 cut(s) 120
HspAI GCGC 6 cut(s) 524, 628, 712, 907, 2632, 2721
Kpn2I TCCGGA 1 cut(s) 278
KpnI GGTACC 2 cut(s) 1815, 1937
KroI GCCGGC 1 cut(s) 674
KroNI GCCGGC 1 cut(s) 676
Ksp22I TGATCA 2 cut(s) 133, 1390
KspAI GTTAAC 2 cut(s) 562, 1857
LguI GCTCTTC 1 cut(s) 943
LmnI GCTCC 2 cut(s) 2746, 2848
MaeI CTAG 3 cut(s) 758, 1862, 2033
MaeII ACGT 8 cut(s) 15, 507, 746, 1957, 2040, 2328, 2466, 2849
MaeIII GTNAC 8 cut(s) 503, 814, 1039, 1090, 1147, 1613, 1784, 2203
MbiI CCGCTC 1 cut(s) 2155
MflI RGATCY 3 cut(s) 809, 2012, 2599
MhlI GDGCHC 1 cut(s) 39
MluI ACGCGT 1 cut(s) 248
MlyI GAGTC 4 cut(s) 1037, 1111, 1289, 1819
Mph1103I ATGCAT 2 cut(s) 1069, 2668
MroI TCCGGA 1 cut(s) 278
MroNI GCCGGC 1 cut(s) 674
MroXI GAANNNNTTC 3 cut(s) 15, 694, 2169
MslI CAYNNNNRTG 1 cut(s) 2511
MspA1I CMGCKG 3 cut(s) 1124, 1778, 1907
MspCI CTTAAG 1 cut(s) 286
MspI CCGG 4 cut(s) 235, 279, 584, 675
MspR9I CCNGG 4 cut(s) 681, 1277, 1686, 2321
Mva1269I GAATGC 1 cut(s) 1133
MvaI CCWGG 4 cut(s) 681, 1277, 1686, 2321
MvnI CGCG 5 cut(s) 250, 630, 714, 2281, 2721
MwoI GCNNNNNNNGC 8 cut(s) 86, 190, 521, 580, 1634, 1661, 1730, 2353
NaeI GCCGGC 1 cut(s) 676
NcoI CCATGG 2 cut(s) 565, 2506
NgoMIV GCCGGC 1 cut(s) 674
NlaIV GGNNCC 4 cut(s) 301, 1813, 1935, 2844
NmuCI GTSAC 7 cut(s) 503, 814, 1090, 1147, 1613, 1784, 2203
NruI TCGCGA 1 cut(s) 2281
NsiI ATGCAT 2 cut(s) 1069, 2668
NspI RCATGY 3 cut(s) 583, 893, 2564
PaeR7I CTCGAG 1 cut(s) 902
PagI TCATGA 1 cut(s) 2359
PciSI GCTCTTC 1 cut(s) 943
PcsI WCGNNNNNNNCGW 1 cut(s) 447
PctI GAATGC 1 cut(s) 1133
PdiI GCCGGC 1 cut(s) 676
PdmI GAANNNNTTC 3 cut(s) 15, 694, 2169
PfeI GAWTC 8 cut(s) 174, 305, 326, 374, 554, 1000, 1348, 2529
PleI GAGTC 4 cut(s) 1036, 1111, 1288, 1819
PpsI GAGTC 4 cut(s) 1036, 1111, 1288, 1819
PsiI TTATAA 1 cut(s) 1532
Psp1406I AACGTT 1 cut(s) 15
Psp6I CCWGG 4 cut(s) 679, 1275, 1684, 2319
PspGI CCWGG 4 cut(s) 679, 1275, 1684, 2319
PspN4I GGNNCC 4 cut(s) 301, 1813, 1935, 2844
PspPI GGNCC 4 cut(s) 88, 300, 677, 1494
PspXI VCTCGAGB 1 cut(s) 902
PstI CTGCAG 2 cut(s) 2479, 2713
PstNI CAGNNNCTG 2 cut(s) 361, 557
PsuI RGATCY 3 cut(s) 809, 2012, 2599
PvuII CAGCTG 2 cut(s) 1778, 1907
RruI TCGCGA 1 cut(s) 2281
RsaI GTAC 9 cut(s) 239, 657, 853, 1813, 1935, 1943, 2027, 2335, 2885
RsaNI GTAC 9 cut(s) 238, 656, 852, 1812, 1934, 1942, 2026, 2334, 2884
RseI CAYNNNNRTG 1 cut(s) 2511
SapI GCTCTTC 1 cut(s) 943
Sau96I GGNCC 4 cut(s) 88, 300, 677, 1494
SchI GAGTC 4 cut(s) 1037, 1111, 1289, 1819
ScrFI CCNGG 4 cut(s) 681, 1277, 1686, 2321
SduI GDGCHC 1 cut(s) 39
SfcI CTRYAG 4 cut(s) 774, 1014, 2475, 2709
Sfr274I CTCGAG 1 cut(s) 902
SinI GGWCC 2 cut(s) 300, 1494
SlaI CTCGAG 1 cut(s) 902
SmiMI CAYNNNNRTG 1 cut(s) 2511
SmlI CTYRAG 3 cut(s) 286, 902, 1949
SmoI CTYRAG 3 cut(s) 286, 902, 1949
SspMI CTAG 3 cut(s) 758, 1862, 2033
StyD4I CCNGG 4 cut(s) 679, 1275, 1684, 2319
StyI CCWWGG 5 cut(s) 6, 84, 565, 1837, 2506
TaaI ACNGT 4 cut(s) 1941, 1946, 2627, 2839
TaiI ACGT 8 cut(s) 18, 510, 749, 1960, 2043, 2331, 2469, 2852
TaqII GACCGA 2 cut(s) 317, 1482
TatI WGTACW 3 cut(s) 851, 2333, 2883
TauI GCSGC 3 cut(s) 83, 518, 529
TfiI GAWTC 8 cut(s) 174, 305, 326, 374, 554, 1000, 1348, 2529
TscAI CASTG 3 cut(s) 271, 2539, 2643
TseFI GTSAC 7 cut(s) 503, 814, 1090, 1147, 1613, 1784, 2203
Tsp45I GTSAC 7 cut(s) 503, 814, 1090, 1147, 1613, 1784, 2203
TspGWI ACGGA 2 cut(s) 1589, 2373
TspRI CASTG 3 cut(s) 271, 2539, 2643
Vha464I CTTAAG 1 cut(s) 286
VpaK11BI GGWCC 2 cut(s) 300, 1494
XagI CCTNNNNNAGG 1 cut(s) 1819
XapI RAATTY 9 cut(s) 70, 393, 917, 1161, 1803, 2167, 2295, 2459, 2489
XceI RCATGY 3 cut(s) 583, 893, 2564
XhoI CTCGAG 1 cut(s) 902
XmnI GAANNNNTTC 3 cut(s) 15, 694, 2169
XspI CTAG 3 cut(s) 758, 1862, 2033
Zsp2I ATGCAT 2 cut(s) 1069, 2668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.